On 11-13 June 2024, the Quadripartite One Health Seminar for the European Region took place in Vienna, Austria. The seminar aimed to discuss and identify opportunities and best practices for operationalizing the One Health approach to address health threats originating at the human-animal-environment interface in Europe and Central Asia, leveraging the One Health Joint Plan of Action and its Implementation Guide. The Seminar was attended by over 150 participants representing Member States, partner organizations and others with an interest in promoting the One Health approach. The Seminar included a meeting between the regional quadripartite and partners. The meeting discussed criteria for selecting priority countries in need of support, modalities and collaborative efforts to enhance coordination of support to Member States, and next steps for enhanced cooperation and coordination of country support activities among the Quadripartite and partners.