A national One Health Joint Plan of Action (OH JPA) workshop was organized and facilitated by the Asia Pacific Quadripartite One Health team from 27-29 February 2024 in Dhaka. The objective of the workshop was to support the ongoing One Health initiatives of Bangladesh, led by the One Health Secretariat, and update the current One Health Strategy Framework 2017-2021 for 2024-2030, aligning and harmonizing it with the global OH JPA and incorporating lessons learned from the COVID-19 pandemic. While Bangladesh has strong institutionalized One Health governance structures and has implemented two series of OH strategic plans. Nonetheless, officials appreciated the value of expanding OH scope beyond zoonoses. Since it was also the first country to be piloted in the region, the lessons learned from this workshop will be used to refine the agenda and methodology for other countries in the pipeline.