The IHR-PVS NBW (global One Health flagship program), jointly organized by the One Health Secretariat of Nepal, FAO, WHO, and WOAH was held from 19-21 March 2024 at the Gokarna Forest Resort of Kathmandu. The workshop was graced by Mr Upendra Yadav, Deputy Prime Minister and Minister for Health and Population (MoHP). Also in attendance were the Minister of Agriculture and Livestock Development (MOALD), Secretaries and Director Generals, Representatives of FAO, WHO and WOAH. The event marked the Federal One Health Steering Committee and the Technical Coordination Committee chairpersonship transition from the MOHP to the MOALD, demonstrating the high-level political commitment to One Health in Nepal. During the workshop, the interactive NBW methodology engaged 134 participants from key One Health national institutions of all three administrative levels, as well as participants from FAO, WHO, WOAH, United Nations Children’s Fund, and United States Agency for International Development (USAID). The workshop enhanced the importance of the One Health approach to address emerging infectious diseases, zoonoses, AMR, food safety, and environmental health. The critical gaps identified during the workshop form the basis for institutionalizing and strengthening the implementation of the One Health approach at national level.