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WTS Database
The subject of interest is:
waterpipe, water-pipe, nargile, narghile, arguileh, arguile, shisha, sheesha, chichi, hubble bubble, hubbly bubbly, goza, hookah, bong
| Title | Pub Year Sort ascending | Author | SearchLink |
|---|---|---|---|
| Antifungal susceptibility of Candida isolates at one institution | 2014 | National Cerebral and Cardiovascular Center. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Medical mycology journal
Periodical, Abbrev.
Med.Mycol.J.
Pub Date Free Form
Volume
55
Issue
1
Start Page
E1
Other Pages
7
Notes
JID: 101562838; 0 (Antifungal Agents); 0 (Echinocandins); 0 (Lipopeptides); 304NUG5GF4 (Itraconazole); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); D83282DT06 (Flucytosine); JFU09I87TR (Voriconazole); R10H71BSWG (micafungin); ppublish
Place of Publication
Japan
ISSN/ISBN
1882-0476
Accession Number
PMID: 24682093
Language
eng
SubFile
Journal Article; IM
DOI
DN/JST.JSTAGE/mmj/55.E1 [pii]
Output Language
Unknown(0)
PMID
24682093
Abstract
Species distribution and antifungal susceptibility of Candida isolates at one institution were evaluated. Detection rates of fungi were examined for 5 years between 2007 and 2011. Sensitivities of fungi to amphotericin B, flucytosine, fluconazole, micafungin, itraconazole, and voriconazole were evaluated in blood culture-positive patients. A total of 3,832 fungal isolates were detected, including Candida albicans 66.5%, Candida glabrata 20.3%, Candida parapsilosis 6.2%, Candida tropicalis 5.5%, and others 1.5%. Candidemia was diagnosed in 131 patients, and C. albicans, C. parapsilosis, C. glabrata, C. tropicalis, and others were present in 42.0%, 27.5%, 16.0%, 8.4%, and 6.1% of these patients, respectively. Voriconazole had the lowest MIC90s against C. albicans and C. parapsilosis (0.015 and 0.25). Micafungin had a low MIC90 against C. glabrata and C. tropicalis. C. albicans was the most common fungus in patients with candidemia. Voriconazole and micafungin were effective against C. albicans. Amphotericin B was effective for C. parapsilosis, and micafungin showed good efficacy against C. glabrata and C. tropicalis.
Descriptors
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Book Title
Database
Publisher
Data Source
Authors
Katsuragi,S., Sata,M., Kobayashi,Y., Miyoshi,T., Yamashita,Y., Neki,R., Horiuchi,C., Yamanaka,K., Kamiya,C., Iwanaga,N., Tanaka,H., Ikeda,T., Yoshimatsu,J.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
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| Update: Ebola virus disease outbreak--West Africa, October 2014 | 2014 | Read more... | |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
MMWR.Morbidity and mortality weekly report
Periodical, Abbrev.
MMWR Morb.Mortal.Wkly.Rep.
Pub Date Free Form
31-Oct
Volume
63
Issue
43
Start Page
978
Other Pages
981
Notes
JID: 7802429; ppublish
Place of Publication
United States
ISSN/ISBN
1545-861X; 0149-2195
Accession Number
PMID: 25356606
Language
eng
SubFile
Journal Article; IM
DOI
mm6343a3 [pii]
Output Language
Unknown(0)
PMID
25356606
Abstract
CDC is assisting ministries of health and working with other organizations to control and end the ongoing outbreak of Ebola virus disease (Ebola) in West Africa. The updated data in this report were compiled from situation reports from the Guinea Interministerial Committee for Response Against the Ebola Virus and the World Health Organization, the Liberia Ministry of Health and Social Welfare, and the Sierra Leone Ministry of Health and Sanitation. Total case counts include all suspected, probable, and confirmed cases as defined by each country. These data reflect reported cases, which make up an unknown proportion of all actual cases and reporting delays that vary from country to country.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Incident Management System Ebola Epidemiology Team, CDC, Guinea Interministerial Committee for Response Against the Ebola Virus, CDC Guinea Response Team, Liberia Ministry of Health and Social Welfare, CDC Liberia Response Team, Sierra Leone Ministry of Health and Sanitation, CDC Sierra Leone Response Team, Viral Special Pathogens Branch, National Center for Emerging and Zoonotic Infectious Diseases, CDC, Centers for Disease Control and Prevention (CDC)
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
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| Relationships among factual and perceived knowledge of harms of waterpipe tobacco, perceived risk, and desire to quit among college users | 2014 | Duke University School of Nursing, USA Isaac.lipkus@duke.edu.; Virginia Commonwealth University, USA.; Duke University Medical Center, USA.; University of Texas, USA.; Duke University School of Nursing, USA. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of health psychology
Periodical, Abbrev.
J.Health.Psychol.
Pub Date Free Form
Dec
Volume
19
Issue
12
Start Page
1525
Other Pages
1535
Notes
LR: 20150601; CI: (c) The Author(s) 2013; GR: P30 DA023026/DA/NIDA NIH HHS/United States; GR: P30 DA023026/DA/NIDA NIH HHS/United States; GR: R01 CA114389/CA/NCI NIH HHS/United States; GR: R01 CA114389/CA/NCI NIH HHS/United States; JID: 9703616; NIHMS6639
Place of Publication
England
ISSN/ISBN
1461-7277; 1359-1053
Accession Number
PMID: 23928987
Language
eng
SubFile
Journal Article; Research Support, N.I.H., Extramural; IM
DOI
10.1177/1359105313494926 [doi]
Output Language
Unknown(0)
PMID
23928987
Abstract
Waterpipe tobacco smoking is increasing in the United States among college students. Through a web-based survey, we explored associations among factual and perceived knowledge, perceived risks and worry about harm and addiction, and desire to quit among 316 college waterpipe tobacco smoking users. Overall, factual knowledge of the harm of waterpipe tobacco smoking was poor, factual and perceived knowledge was weakly correlated, both forms of knowledge were related inconsistently to perceived risks and worry, and neither form of knowledge was associated with the desire to quit. Findings provide preliminary insights as to why knowledge gaps may not predict cessation among waterpipe users.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Lipkus,I.M., Eissenberg,T., Schwartz-Bloom,R.D., Prokhorov,A.V., Levy,J.
Original/Translated Title
URL
Date of Electronic
20130808
PMCID
PMC4358735
Editors
|
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| Quit history, intentions to quit, and reasons for considering quitting among tobacco users in India: findings from the Tobacco Control Policy Evaluation India Wave 1 Survey | 2014 | Healis Sekhsaria Institute for Public Health, Navi Mumbai, Maharashtra, India. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Indian journal of cancer
Periodical, Abbrev.
Indian J.Cancer
Pub Date Free Form
Dec
Volume
51 Suppl 1
Issue
Start Page
S39
Other Pages
45
Notes
LR: 20151028; GR: 115216/Canadian Institutes of Health Research/Canada; GR: 79551/Canadian Institutes of Health Research/Canada; GR: P01 CA138389/CA/NCI NIH HHS/United States; GR: P01-CA138389/CA/NCI NIH HHS/United States; JID: 0112040; NIHMS708516; OID:
Place of Publication
India
ISSN/ISBN
1998-4774; 0019-509X
Accession Number
PMID: 25526247
Language
eng
SubFile
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; IM
DOI
10.4103/0019-509X.147467 [doi]
Output Language
Unknown(0)
PMID
25526247
Abstract
BACKGROUND: Global Adult Tobacco Survey India 2009-2010 revealed that more than one-third (35%) of adults in India use tobacco in some form: 21% use smokeless tobacco, 9% smoke, and 5% are mixed users (they smoke and use smokeless tobacco), and the quit rate is very low. In an effort to decrease prevalence of tobacco use, it is thus important to understand the factors that are related to intention to quit among Indian tobacco users. Research has shown consistently that intention to quit is a strong predictor of future quitting. The present study reports the factors encouraging quitting tobacco products in India. SUBJECTS AND METHODS: Cross-sectional data from Wave 1 of the International Tobacco Control Policy Evaluation India Survey conducted in four cities and surrounding rural areas (i.e. Mumbai [Maharashtra], Patna [Bihar], Indore [Madhya Pradesh], and Kolkata [West Bengal]) between August 2010 and December 2011 were analyzed. A total of 8051 tobacco users (15+ years) were randomly sampled from 8586 households: 1255 smokers, 5991 smokeless users, and 805 mixed (smoke and smokeless) users. Validated, standardized questions were asked about current tobacco use, intention to quit, and factors encouraging quitting. RESULTS: Overall, 19.6% of tobacco users intended to quit. Smokers had less intention to quit as compared to smokeless tobacco users whereas mixed users had more intention to quit (odds ratio [OR] =1.48, 95% confidence interval [CI] =1.12-1.97) compared to smokeless tobacco users. Highly educated people were more likely to report intention to quit (OR = 1.82, 95% CI = 1.09-3.02) compared to less educated. Advice by doctors to quit tobacco had a strong impact on intention to quit (OR = 1.68, CI = 1.29-2.15). Tobacco users who were exposed to antitobacco messages at work places (OR = 1.74, CI = 1.23-2.46), at restaurants (OR = 1.65, CI = 1.12-2.43), bars (OR = 1.81, CI = 1.07-3.06), on public transportation (OR = 2.14, CI = 1.49-3.08) and on tobacco packages (OR = 1.77, CI = 1.29-2.14) also expressed greater intention to quit tobacco use. CONCLUSION: Around one-fifth of tobacco users in India intended to quit tobacco use. Higher education, doctor's advice, and antitobacco messages were positively associated with users' intention to quit tobacco.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Dhumal,G.G., Pednekar,M.S., Gupta,P.C., Sansone,G.C., Quah,A.C., Bansal-Travers,M., Fong,G.T.
Original/Translated Title
URL
Date of Electronic
PMCID
PMC4527990
Editors
|
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| Depression in smoking pregnant women: impact on motivation to quit smoking | 2014 | Service de maternite, groupe hospitalier mutualiste de Grenoble, 38000 Grenoble, France.; Institut Rhone-Alpes Auvergne de tabacologie (IRAAT), hopital de la Croix-Rousse, 103, grande rue de la Croix-Rousse, 69004 Lyon, France; Dispensaire Emile-Roux, 11, | Read more... |
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Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal de gynecologie, obstetrique et biologie de la reproduction
Periodical, Abbrev.
J.Gynecol.Obstet.Biol.Reprod.(Paris)
Pub Date Free Form
Nov
Volume
43
Issue
9
Start Page
691
Other Pages
697
Notes
CI: Copyright (c) 2013; JID: 0322206; OTO: NOTNLM; 2013/07/01 [received]; 2013/09/09 [revised]; 2013/09/13 [accepted]; 2013/10/14 [aheadofprint]; ppublish
Place of Publication
France
ISSN/ISBN
1773-0430; 0150-9918
Accession Number
PMID: 24135016
Language
fre
SubFile
English Abstract; Journal Article; Multicenter Study; IM
DOI
10.1016/j.jgyn.2013.09.005 [doi]
Output Language
Unknown(0)
PMID
24135016
Abstract
Despite the organization of smoking cessation program, the percentage of pregnant smokers remains too high in France. The knowledge of the factors limiting success of the attempt can help the smoking cessation. AIM: To evaluate the prevalence of depressive disorders in pregnant smokers compared to nonsmokers; assess their impact on motivation stopping smoking; verify their identification can be performed by midwives. MATERIALS AND METHODS: Prospective multicenter survey on 792 women (435 smokers and 357 non-smokers), the assessment of the current depression is made by the Hospital Anxiety Depression scale, and motivation to quit smoking by the Richmond test. RESULTS: Pregnant smokers compared to non-smokers, have more frequent depressive disorders (current: OR=2.4; history: OR=2). These problems do not decrease the motivation to quit, they are associated with a high nicotine dependence and low socioeconomic levels. Their testing can be done by midwives. CONCLUSION: Systematic screening for depression in pregnant women smoking could facilitate smoking cessation.
Descriptors
Links
Book Title
Database
Publisher
Elsevier Masson SAS
Data Source
Authors
Dupre,F., Perriot,J., Defay,I., Lavessiere,C., Defay,F., Guillon,C., Mathern,G., Berland,M.
Original/Translated Title
Depression chez la femme enceinte fumeuse : impact sur la motivation a l'arret du tabac
URL
Date of Electronic
20131014
PMCID
Editors
|
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| Secondhand smoke exposure at home: assessment by biomarkers and airborne markers | 2014 | Tobacco Control Unit, Cancer Prevention and Control Program, Institut Catala d'Oncologia, Av. Granvia de l'Hospitalet, 08908 L'Hospitalet de Llobregat, 199-203 Barcelona, Spain; Cancer Prevention and Control Group, Institut d'Investigacio Biomedica de Bel | Read more... |
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Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Environmental research
Periodical, Abbrev.
Environ.Res.
Pub Date Free Form
Aug
Volume
133
Issue
Start Page
111
Other Pages
116
Notes
LR: 20151119; CI: Copyright (c) 2014; JID: 0147621; 0 (Biomarkers); 0 (Tobacco Smoke Pollution); 6M3C89ZY6R (Nicotine); J64922108F (Benzene); K5161X06LL (Cotinine); OTO: NOTNLM; 2014/03/22 [received]; 2014/05/05 [revised]; 2014/05/07 [accepted]; 2014/06/0
Place of Publication
United States
ISSN/ISBN
1096-0953; 0013-9351
Accession Number
PMID: 24912142
Language
eng
SubFile
Journal Article; Observational Study; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.envres.2014.05.013 [doi]
Output Language
Unknown(0)
PMID
24912142
Abstract
OBJECTIVE: We assessed and characterized the relationship among biomarkers of secondhand smoke (SHS) exposure in non-smokers according to their exposure at home as measured by airborne markers. METHODS: We conducted an observational study on exposure to SHS at home using airborne markers (nicotine and benzene) and biomarkers from the non-smokers living in these homes. We selected 49 non-smoking volunteers from different homes: 25 non-smokers living with at least one smoker and 24 non-smokers living in smoke-free homes. We installed two passive devices to measure nicotine and benzene concentrations in the main room of the house (i.e., the living room). One week later, the researcher returned to the volunteer's home to collect the two devices, obtain saliva and urine samples, and administer a SHS questionnaire. RESULTS: Salivary and urinary cotinine concentrations highly correlated with air nicotine concentrations measured at the volunteers'homes (rsp=0.738 and rsp=0.679, respectively). The concentrations of airborne markers of SHS and biomarkers in non-smokers increased with increasing self-reported intensity and duration of SHS exposure at home during the previous week (p
Descriptors
Links
Book Title
Database
Publisher
Elsevier Inc
Data Source
Authors
Martinez-Sanchez,J.M., Sureda,X., Fu,M., Perez-Ortuno,R., Ballbe,M., Lopez,M.J., Salto,E., Pascual,J.A., Fernandez,E.
Original/Translated Title
URL
Date of Electronic
20140607
PMCID
Editors
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| Candida species distribution and antifungal susceptibility testing according to European Committee on Antimicrobial Susceptibility Testing and new vs. old Clinical and Laboratory Standards Institute clinical breakpoints: a 6-year prospective candidaemia s | 2014 | Infectious Diseases Service, Department of Medicine, Lausanne University Hospital, Lausanne, Switzerland. | Read more... |
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Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
Periodical, Abbrev.
Clin.Microbiol.Infect.
Pub Date Free Form
Jul
Volume
20
Issue
7
Start Page
698
Other Pages
705
Notes
CI: (c) 2013 The Authors Clinical Microbiology and Infection (c) 2013; JID: 9516420; 0 (Antifungal Agents); 0 (Echinocandins); 8VZV102JFY (Fluconazole); F0XDI6ZL63 (caspofungin); JFU09I87TR (Voriconazole); OTO: NOTNLM; 2013/07/18 [received]; 2013/10/28 [r
Place of Publication
France
ISSN/ISBN
1469-0691; 1198-743X
Accession Number
PMID: 24188136
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1111/1469-0691.12440 [doi]
Output Language
Unknown(0)
PMID
24188136
Abstract
We analyzed the species distribution of Candida blood isolates (CBIs), prospectively collected between 2004 and 2009 within FUNGINOS, and compared their antifungal susceptibility according to clinical breakpoints defined by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) in 2013, and the Clinical and Laboratory Standards Institute (CLSI) in 2008 (old CLSI breakpoints) and 2012 (new CLSI breakpoints). CBIs were tested for susceptiblity to fluconazole, voriconazole and caspofungin by microtitre broth dilution (Sensititre(R) YeastOne test panel). Of 1090 CBIs, 675 (61.9%) were C. albicans, 191 (17.5%) C. glabrata, 64 (5.9%) C. tropicalis, 59 (5.4%) C. parapsilosis, 33 (3%) C. dubliniensis, 22 (2%) C. krusei and 46 (4.2%) rare Candida species. Independently of the breakpoints applied, C. albicans was almost uniformly (>98%) susceptible to all three antifungal agents. In contrast, the proportions of fluconazole- and voriconazole-susceptible C. tropicalis and F-susceptible C. parapsilosis were lower according to EUCAST/new CLSI breakpoints than to the old CLSI breakpoints. For caspofungin, non-susceptibility occurred mainly in C. krusei (63.3%) and C. glabrata (9.4%). Nine isolates (five C. tropicalis, three C. albicans and one C. parapsilosis) were cross-resistant to azoles according to EUCAST breakpoints, compared with three isolates (two C. albicans and one C. tropicalis) according to new and two (2 C. albicans) according to old CLSI breakpoints. Four species (C. albicans, C. glabrata, C. tropicalis and C. parapsilosis) represented >90% of all CBIs. In vitro resistance to fluconazole, voriconazole and caspofungin was rare among C. albicans, but an increase of non-susceptibile isolates was observed among C. tropicalis/C. parapsilosis for the azoles and C. glabrata/C. krusei for caspofungin according to EUCAST and new CLSI breakpoints compared with old CLSI breakpoints.
Descriptors
Links
Book Title
Database
Publisher
European Society of Clinical Microbiology and Infectious Diseases
Data Source
Authors
Orasch,C., Marchetti,O., Garbino,J., Schrenzel,J., Zimmerli,S., Muhlethaler,K., Pfyffer,G., Ruef,C., Fehr,J., Zbinden,R., Calandra,T., Bille,J.
Original/Translated Title
URL
Date of Electronic
20131212
PMCID
Editors
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| The relationship of decongestant use and risk of decompression sickness; a case-control study of Hawaiian scuba divers | 2014 | Hyperbaric Treatment Center, University of Hawai'i, John A. Burns School of Medicine, Honolulu, HI. | Read more... |
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Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Hawai'i journal of medicine & public health : a journal of Asia Pacific Medicine & Public Health
Periodical, Abbrev.
Hawaii.J.Med.Public.Health.
Pub Date Free Form
Feb
Volume
73
Issue
2
Start Page
61
Other Pages
65
Notes
LR: 20150515; JID: 101579076; OID: NLM: PMC3931412; OTO: NOTNLM; ppublish
Place of Publication
United States
ISSN/ISBN
2165-8242; 2165-8242
Accession Number
PMID: 24567870
Language
eng
SubFile
Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
24567870
Abstract
Exposure to cold, dehydration, and aging are known to contribute to the development of decompression sickness (DCS) in divers. Hypertension and nicotine usage have also been suggested as risk factors. Vasoconstriction is an underlying mechanism associated with all of these risk factors. Vasoconstriction increases the degree of bubble formation which is believed to be the cause of DCS. Formed bubbles interfere with the production of nitric oxide which modulates vascular tone resulting in vasoconstriction. Divers commonly use sympathomimetic decongestants which induce vasoconstriction to prevent barotrauma of the ears and sinuses while diving and thus theoretically may contribute to the risk for developing DCS. The purpose of this case-control study was to explore the association between decongestant usage and development of DCS in 400 divers treated/evaluated at the University of Hawai'i, John A. Burns School of Medicine between 1983 and 2010. Bivariate and logistic regression analyses were employed to evaluate differences between cases and controls. In addition to the variable of interest, other co-variables known to have significant influence in the development of DCS were appropriately controlled for during the analyses. In this study population, dehydration (OR = 2.7; 95% CI: 1.1, 7.4), repetitive diving (OR = 2.8; 95% CI: 1.8, 4.4), and violation of dive profiles (OR = 4.9; 95% CI: 3.1, 7.9) contributed independently and significantly to the development of DCS. The co-variables of cold, gender, obesity, and rapid ascents were not significant contributors to developing DCS in this study. There was a small but statistically insignificant risk associated with decongestant use (OR = 1.4; 95% CI: 0.8-2.6; P = .22). The inherent limitations associated with records-based studies may have underestimated this risk. It is important therefore that future research be undertaken to help clarify this concern.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Smerz,R.W.
Original/Translated Title
URL
Date of Electronic
PMCID
PMC3931412
Editors
|
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| Diving medicine | 2014 | Cardiology Section, Temple University School of Medicine, Philadelphia, Pennsylvania. | Read more... |
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Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
American journal of respiratory and critical care medicine
Periodical, Abbrev.
Am.J.Respir.Crit.Care Med.
Pub Date Free Form
15-Jun
Volume
189
Issue
12
Start Page
1479
Other Pages
1486
Notes
JID: 9421642; N762921K75 (Nitrogen); S88TT14065 (Oxygen); OTO: NOTNLM; ppublish
Place of Publication
United States
ISSN/ISBN
1535-4970; 1073-449X
Accession Number
PMID: 24869752
Language
eng
SubFile
Journal Article; Review; AIM; IM
DOI
10.1164/rccm.201309-1662CI [doi]
Output Language
Unknown(0)
PMID
24869752
Abstract
Exposure to the undersea environment has unique effects on normal physiology and can result in unique disorders that require an understanding of the effects of pressure and inert gas supersaturation on organ function and knowledge of the appropriate therapies, which can include recompression in a hyperbaric chamber. The effects of Boyle's law result in changes in volume of gas-containing spaces when exposed to the increased pressure underwater. These effects can cause middle ear and sinus injury and lung barotrauma due to lung overexpansion during ascent from depth. Disorders related to diving have unique presentations, and an understanding of the high-pressure environment is needed to properly diagnose and manage these disorders. Breathing compressed air underwater results in increased dissolved inert gas in tissues and organs. On ascent after a diving exposure, the dissolved gas can achieve a supersaturated state and can form gas bubbles in blood and tissues, with resulting tissue and organ damage. Decompression sickness can involve the musculoskeletal system, skin, inner ear, brain, and spinal cord, with characteristic signs and symptoms. Usual therapy is recompression in a hyperbaric chamber following well-established protocols. Many recreational diving candidates seek medical clearance for diving, and healthcare providers must be knowledgeable of the environmental exposure and its effects on physiologic function to properly assess individuals for fitness to dive. This review provides a basis for understanding the diving environment and its accompanying disorders and provides a basis for assessment of fitness for diving.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Bove,A.A.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
|
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| Saccharomyces cerevisiae biofilm tolerance towards systemic antifungals depends on growth phase | 2014 | Department of Systems Biology, Technical University of Denmark, Kgs, Lyngby, Denmark. rakb@vet.dtu.dk. | Read more... |
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Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
BMC microbiology
Periodical, Abbrev.
BMC Microbiol.
Pub Date Free Form
4-Dec
Volume
14
Issue
Start Page
305
Other Pages
014-0305-4
Notes
LR: 20151028; JID: 100966981; 0 (Antifungal Agents); OID: NLM: PMC4258017; 2014/07/25 [received]; 2014/11/20 [accepted]; 2014/12/04 [aheadofprint]; epublish
Place of Publication
England
ISSN/ISBN
1471-2180; 1471-2180
Accession Number
PMID: 25472667
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1186/s12866-014-0305-4 [doi]
Output Language
Unknown(0)
PMID
25472667
Abstract
BACKGROUND: Biofilm-forming Candida species cause infections that can be difficult to eradicate, possibly because of antifungal drug tolerance mechanisms specific to biofilms. In spite of decades of research, the connection between biofilm and drug tolerance is not fully understood. RESULTS: We used Saccharomyces cerevisiae as a model for drug susceptibility of yeast biofilms. Confocal laser scanning microscopy showed that S. cerevisiae and C. glabrata form similarly structured biofilms and that the viable cell numbers were significantly reduced by treatment of mature biofilms with amphotericin B but not voriconazole, flucytosine, or caspofungin. We showed that metabolic activity in yeast biofilm cells decreased with time, as visualized by FUN-1 staining, and mature, 48-hour biofilms contained cells with slow metabolism and limited growth. Time-kill studies showed that in exponentially growing planktonic cells, voriconazole had limited antifungal activity, flucytosine was fungistatic, caspofungin and amphotericin B were fungicidal. In growth-arrested cells, only amphotericin B had antifungal activity. Confocal microscopy and colony count viability assays revealed that the response of growing biofilms to antifungal drugs was similar to the response of exponentially growing planktonic cells. The response in mature biofilm was similar to that of non-growing planktonic cells. These results confirmed the importance of growth phase on drug efficacy. CONCLUSIONS: We showed that in vitro susceptibility to antifungal drugs was independent of biofilm or planktonic growth mode. Instead, drug tolerance was a consequence of growth arrest achievable by both planktonic and biofilm populations. Our results suggest that efficient strategies for treatment of yeast biofilm might be developed by targeting of non-dividing cells.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Bojsen,R., Regenberg,B., Folkesson,A.
Original/Translated Title
URL
Date of Electronic
20141204
PMCID
PMC4258017
Editors
|
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