WTS database

Title	Pub Year Sort ascending	Author	SearchLink
Enhanced motivational interviewing versus brief advice for adolescent smoking cessation: results from a randomized clinical trial	2012	Center for Alcohol and Addiction Studies, Brown University, Box G-S121, Providence, RI 02912, USA. Suzanne_Colby@brown.edu	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Addictive Behaviors Periodical, Abbrev. Addict.Behav. Pub Date Free Form Jul Volume 37 Issue 7 Start Page 817 Other Pages 823 Notes LR: 20150225; CI: Copyright (c) 2012; GR: 1R01 AA016000/AA/NIAAA NIH HHS/United States; GR: 1R01 DA11204/DA/NIDA NIH HHS/United States; GR: 1T32 DA016184/DA/NIDA NIH HHS/United States; GR: K05 AA019681/AA/NIAAA NIH HHS/United States; GR: R01 AA016000/AA/N Place of Publication England ISSN/ISBN 1873-6327; 0306-4603 Accession Number PMID: 22472523 Language eng SubFile Journal Article; Randomized Controlled Trial; Research Support, N.I.H., Extramural; IM DOI 10.1016/j.addbeh.2012.03.011 [doi] Output Language Unknown(0) PMID 22472523 Abstract BACKGROUND: Motivational interviewing (MI) is widely used for adolescent smoking cessation but empirical support for this approach is mixed. METHODS: Adolescent cigarette smokers 14-18 years old (N=162) were recruited from medical, school, and community settings and randomly assigned to enhanced MI or brief advice (BA) for smoking cessation. MI comprised an in-person individual session, a telephone booster session one week later, and a brief telephone-based parent intervention. BA consisted of standardized brief advice to quit smoking. Assessments occurred at baseline, post-treatment and at 1-, 3-, and 6-month follow ups. RESULTS: Biochemically-confirmed 7-day point prevalence abstinence rates were low (e.g., 4.5% for MI; 1.4% for BA at 1 month) and did not differ significantly by group at any follow up. Only those in MI reported significant decreases in cigarettes smoked per day (CPD) from baseline to 1 month. At 3 and 6 months, smokers in both groups reported significantly reduced CPD with no differences between groups. MI reduced perceived norms regarding peer and adult smoking rates, while BA had no effect on normative perceptions. No group differences emerged for self-reported motivation or self-efficacy to quit smoking. CONCLUSIONS: Findings support the efficacy of MI for addressing normative misperceptions regarding peer and adult smoking and for modestly reducing CPD in the short-term; however, these effects did not translate to greater smoking abstinence. MI may have more promise as a prelude to more intensive smoking intervention with adolescents than as a stand-alone intervention. Descriptors Links Book Title Database Publisher Elsevier Ltd Data Source Authors Colby,S.M., Nargiso,J., Tevyaw,T.O., Barnett,N.P., Metrik,J., Lewander,W., Woolard,R.H., Rohsenow,D.J., Monti,P.M. Original/Translated Title URL Date of Electronic 20120316 PMCID PMC3356495 Editors
Evaluation of fast volatile analysis for detection of Botrytis cinerea infections in strawberry	2012	BIOSYST-MeBioS, Katholieke Universiteit Leuven, Willem de Croylaan 42, 3001 Leuven, Belgium.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Food Microbiology Periodical, Abbrev. Food Microbiol. Pub Date Free Form Dec Volume 32 Issue 2 Start Page 406 Other Pages 414 Notes CI: Copyright (c) 2012; JID: 8601127; 0 (Volatile Organic Compounds); 2011/10/21 [received]; 2012/07/04 [revised]; 2012/08/06 [accepted]; 2012/08/11 [aheadofprint]; ppublish Place of Publication England ISSN/ISBN 1095-9998; 0740-0020 Accession Number PMID: 22986207 Language eng SubFile Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1016/j.fm.2012.08.002 [doi] Output Language Unknown(0) PMID 22986207 Abstract Grey mold (Botrytis cinerea) is one of the major phytopathogens causing serious losses during strawberry postharvest and storage. B. cinerea-host interaction affect emissions of volatile compounds during infection resulting in a characteristic earthy, mushroom odor. Therefore, the objective of this study was to evaluate two analytical techniques based on fast volatile analysis on their performance for monitoring evolution and early detection of B. cinerea infections in strawberry. In a first experiment headspace multi-capillary column-ion mobility spectrometry (HS MCC-IMS) has been successfully used to evaluate development of strawberry aroma during shelflife. In a second experiment the same technique has been used to detect the degree of B. cinerea infection through changes in the volatile profile. Additionally, these samples were analyzed with headspace solid-phase-microextraction fast GC-MS (HS SPME fast GC-MS). Both HS MCC-IMS and HS SPME fast GC-MS could determine the changes in volatile composition as a function of the degree of B. cinerea infection as determined by an enzyme-linked immunosorbent assay (ELISA) and could be used to follow the evolution of infection. According to the ELISA data, some fruit were infected even without any symptoms and volatiles produced by the fungus may be overshadowed by the fruit volatiles. Therefore, both analytical techniques could not be used for early detection of B. cinerea infections. After identification of the volatile compounds and multivariate data analysis, potential biomarkers specific for B. cinerea were highlighted, being 3-methylbutanal, cis-4-decenal, 2-methyl-1-butanol, 2-methyl-1-propanol, 1-octen-3-one and 1-octen-3-ol. Descriptors Links Book Title Database Publisher Elsevier Ltd Data Source Authors Vandendriessche,T., Keulemans,J., Geeraerd,A., Nicolai,B.M., Hertog,M.L. Original/Translated Title URL Date of Electronic 20120811 PMCID Editors
Legionella on board trains: effectiveness of environmental surveillance and decontamination	2012	Institute of Hygiene, Universita Cattolica del Sacro Cuore, Lgo F, Vito 1, 00168 Rome, Italy.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full BMC public health Periodical, Abbrev. BMC Public Health Pub Date Free Form 7-Aug Volume 12 Issue Start Page 618 Other Pages 2458-12-618 Notes LR: 20150224; JID: 100968562; OID: NLM: PMC3519711; 2012/01/20 [received]; 2012/07/30 [accepted]; 2012/08/07 [aheadofprint]; epublish Place of Publication England ISSN/ISBN 1471-2458; 1471-2458 Accession Number PMID: 22870945 Language eng SubFile Evaluation Studies; Journal Article; IM DOI 10.1186/1471-2458-12-618 [doi] Output Language Unknown(0) PMID 22870945 Abstract BACKGROUND: Legionella pneumophila is increasingly recognised as a significant cause of sporadic and epidemic community-acquired and nosocomial pneumonia. Many studies describe the frequency and severity of Legionella spp. contamination in spa pools, natural pools, hotels and ships, but there is no study analysing the environmental monitoring of Legionella on board trains. The aims of the present study were to conduct periodic and precise environmental surveillance of Legionella spp. in water systems and water tanks that supply the toilet systems on trains, to assess the degree of contamination of such structures and to determine the effectiveness of decontamination. METHODS: A comparative pre-post ecological study was conducted from September 2006 to January 2011. A total of 1,245 water samples were collected from plumbing and toilet water tanks on passenger trains. The prevalence proportion of all positive samples was calculated. The unpaired t-test was performed to evaluate statistically significant differences between the mean load values before and after the decontamination procedures; statistical significance was set at p = 0.05. RESULTS: In the pre-decontamination period, 58% of the water samples were positive for Legionella. Only Legionella pneumophila was identified: 55.84% were serogroup 1, 19.03% were serogroups 2-14 and 25.13% contained both serogroups. The mean bacterial load value was 2.14 x 10(3) CFU/L. During the post-decontamination period, 42.75% of water samples were positive for Legionella spp.; 98.76% were positive for Legionella pneumophila: 74.06% contained serogroup 1, 16.32% contained serogroups 2-14 and 9.62% contained both. The mean bacterial load in the post-decontamination period was 1.72 x 10(3) CFU/L. According to the t-test, there was a statistically significant decrease in total bacterial load until approximately one and a half year after beginning the decontamination programme (p = 0.0097). CONCLUSIONS: This study indicates that systematic environmental surveillance could be a useful approach for assessing the risk of exposure to Legionella bacteria, which still represents a public health threat. According to the study results, an environmental surveillance programme, followed by decontamination procedures where necessary, would decrease the total bacterial count, protecting the health of travellers and workers. Descriptors Links Book Title Database Publisher Data Source Authors Quaranta,G., Vincenti,S., Ferriero,A.M., Boninti,F., Sezzatini,R., Turnaturi,C., Gliubizzi,M.D., Munafo,E., Ceccarelli,G., Causarano,C., Accorsi,M., Del Nord,P., Ricciardi,W., Laurenti,P. Original/Translated Title URL Date of Electronic 20120807 PMCID PMC3519711 Editors
Prevalence and characterization of extended-spectrum beta-lactamase (ESBL)- and CMY-2-producing Escherichia coli isolates from healthy food-producing animals in Tunisia	2012	Laboratoire Microorganismes et Biomolecules Actives, Faculte des Sciences de Tunis, Universite Tunis-El Manar, Tunis, Tunisia.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Foodborne pathogens and disease Periodical, Abbrev. Foodborne Pathog.Dis. Pub Date Free Form Dec Volume 9 Issue 12 Start Page 1137 Other Pages 1142 Notes JID: 101120121; 0 (Anti-Bacterial Agents); EC 3.5.2.6 (beta-Lactamases); 2012/11/29 [aheadofprint]; ppublish Place of Publication United States ISSN/ISBN 1556-7125; 1535-3141 Accession Number PMID: 23194332 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1089/fpd.2012.1267 [doi] Output Language Unknown(0) PMID 23194332 Abstract The prevalence of extended-spectrum beta-lactamase (ESBL)- and plasmidic AmpC-beta-lactamase (pAmpC-BL)-producing Escherichia coli isolates has been studied in food-producing animals at the farm level in Tunisia, and recovered isolates were characterized for the presence of other resistance genes and integrons. Eighty fecal samples of food-producing animals (23 sheep, 22 chickens, 22 cattle, six horses, five rabbits, and two dromedaries) were obtained from 35 different farms in Tunisia in 2011. Samples were inoculated onto MacConkey agar plates supplemented with cefotaxime (2 mg/L) for cefotaxime-resistant (CTX(R)) E. coli recovery. CTX(R) E. coli isolates were detected in 11 out of 80 samples (13.8%), and one isolate per sample was further characterized (10 from chickens and one from a dromedary). The 11 CTX(R) isolates were distributed into phylogroups: B1 (five isolates), A (two isolates), D (three isolates), and B2 (one isolate). The following beta-lactamase genes were detected: bla(CTX-M-1) (seven isolates), bla(CTX-M-1)+bla(TEM-135) (one isolate), bla(CTX-M-1)+bla(TEM-1b) (one isolate), and bla(CMY-2) (two isolates). All ESBL- and pAmpC-BL-producing E. coli strains showed unrelated pulsed-field gel electrophoresis patterns. Seven isolates contained class 1 integrons with four gene cassette arrangements: dfrA17-aadA5 (three isolates), dfrA1-aadA1 (two isolates), dfrA15-aadA1 (one isolate), and aadA1 (one isolate). All isolates showed tetracycline resistance and contained the tet(A) +/- tet(B) genes. Virulence genes detected were as follows (number of isolates in parentheses): fimA (10); aer (eight); papC (two); and papGIII, hly, cnf, and bfp (none). Chicken farms constitute a reservoir of ESBL- and pAmpC-BL-producing E. coli isolates of the CTX-M-1 and CMY-2 types that potentially could be transmitted to humans via the food chain or by direct contact. Descriptors Links Book Title Database Publisher Data Source Authors Ben Sallem,R., Ben Slama,K., Saenz,Y., Rojo-Bezares,B., Estepa,V., Jouini,A., Gharsa,H., Klibi,N., Boudabous,A., Torres,C. Original/Translated Title URL Date of Electronic 20121129 PMCID Editors
Genetic analysis of strawberry fruit aroma and identification of O-methyltransferase FaOMT as the locus controlling natural variation in mesifurane content	2012	Instituto Andaluz de Investigacion y Formacion Agraria y Pesquera, Centro de Churriana, 29140 Malaga, Spain.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Plant Physiology Periodical, Abbrev. Plant Physiol. Pub Date Free Form Jun Volume 159 Issue 2 Start Page 851 Other Pages 870 Notes LR: 20150225; GENBANK/JQ322651; GENBANK/JQ322652; GENBANK/JQ322653; GENBANK/JQ322654; GENBANK/JQ322655; GENBANK/JQ322656; GENBANK/JQ322657; GENBANK/JQ322658; GENBANK/JQ322659; JID: 0401224; 0 (Flavoring Agents); 0 (Furans); 0 (Volatile Organic Compounds); Place of Publication United States ISSN/ISBN 1532-2548; 0032-0889 Accession Number PMID: 22474217 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1104/pp.111.188318 [doi] Output Language Unknown(0) PMID 22474217 Abstract Improvement of strawberry (Fragaria x ananassa) fruit flavor is an important goal in breeding programs. To investigate genetic factors controlling this complex trait, a strawberry mapping population derived from genotype '1392', selected for its superior flavor, and '232' was profiled for volatile compounds over 4 years by headspace solid phase microextraction coupled to gas chromatography and mass spectrometry. More than 300 volatile compounds were detected, of which 87 were identified by comparison of mass spectrum and retention time to those of pure standards. Parental line '1392' displayed higher volatile levels than '232', and these and many other compounds with similar levels in both parents segregated in the progeny. Cluster analysis grouped the volatiles into distinct chemically related families and revealed a complex metabolic network underlying volatile production in strawberry fruit. Quantitative trait loci (QTL) detection was carried out over 3 years based on a double pseudo-testcross strategy. Seventy QTLs covering 48 different volatiles were detected, with several of them being stable over time and mapped as major QTLs. Loci controlling gamma-decalactone and mesifurane content were mapped as qualitative traits. Using a candidate gene approach we have assigned genes that are likely responsible for several of the QTLs. As a proof of concept we show that one homoeolog of the O-methyltransferase gene (FaOMT) is the locus responsible for the natural variation of mesifurane content. Sequence analysis identified 30 bp in the promoter of this FaOMT homoeolog containing putative binding sites for basic/helix-loop-helix, MYB, and BZIP transcription factors. This polymorphism fully cosegregates with both the presence of mesifurane and the high expression of FaOMT during ripening. Descriptors Links Book Title Database Publisher Data Source Authors Zorrilla-Fontanesi,Y., Rambla,J.L., Cabeza,A., Medina,J.J., Sanchez-Sevilla,J.F., Valpuesta,V., Botella,M.A., Granell,A., Amaya,I. Original/Translated Title URL Date of Electronic 20120403 PMCID PMC3375946 Editors
Impact of cigarette packages warning labels in relation to tobacco-smoking dependence and motivation to quit	2012	Sezione igiene, Dipartimento di sanita pubblica e malattie infettive, Universita Sapienza di Roma, Italy. alice.mannocci@uniroma1.it	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Epidemiologia e prevenzione Periodical, Abbrev. Epidemiol.Prev. Pub Date Free Form Mar-Apr Volume 36 Issue 2 Start Page 100 Other Pages 107 Notes LR: 20151119; JID: 8902507; ppublish Place of Publication Italy ISSN/ISBN 1120-9763; 1120-9763 Accession Number PMID: 22706360 Language ita SubFile English Abstract; Journal Article; Multicenter Study; IM DOI 1306 [pii] Output Language Unknown(0) PMID 22706360 Abstract OBJECTIVE: the principal aim was to assess the impact of health warnings on cigarette packages in Italy, the reduction of daily number of cigarette smoked, in relationship to the tobacco-smoking dependence and motivation to quit. The second aim was to compare the impact of text warnings versus graphi depictions. DESIGN: cross-sectional study (survey). SETTING AND PARTICIPANTS: the study was conducted through interviews to an opportunistic sample of smokers.The subject enrolled were adult smokers (years >/= 18), living in the province of Rome. Data were collected in two outpatient clinics located in Morlupo and Rome. Interviews were administered in the waiting rooms, to patients or to their relatives/ helpers. The survey was conducted in June-September 2010. The sample size (266 participants) was computed using a power of 80%, a confidence level of 95%, an expected frequency of smokers with a low motivation to quit who reduced number of cigarettes due to warnings of 15%, and a frequency of smokers with a higher motivation to quit who reduced number of cigarettes due to warnings of 30%. MAIN OUTCOME MEASURES: the effect of the health warnings used in Italy on smoking reduction was measured with the following self-reported items: "Are you or have you been influenced by the health warnings on cigarettes packages (in relation to the daily number of cigarettes smoked)?"; "Have you changed your smoking habits due to the warnings (for example: don't smoking after a coffee.)?"; "Have you ever stopped smoking due to the warnings?" The effect of labels that used shock images on cigarette boxes was measured using followed self-reported questions: "If shocking images were used on cigarette boxes, would they have greater effect than simple warning text currently used?"; "If your favourite cigarettes brand decide to change the look of its cigarette boxes with shocking images on smoking health damages, would you be driven to change it?" RESULTS: thanks to the health warnings, 95% of the 270 participants were informed on smoking damages, 14% (34 smokers) reduced the number cigarettes per day, and 5% (12 smokers) attempted to quit. Forty-five percent of smokers did not know the real impact of light cigarettes on their health, and 49% decided to smoke light cigarettes after having read the warnings. Women were more susceptible to immediate consequences of smoking (appearance of wrinkles; p/= 45 years (OR=2.54; 95%CI 1.05-6.17), more motivated to quit (OR=2.92; 95%CI 1.17-7.30), those who reported they do not like the smoking smell on their own clothes (OR=3.6; 95%CI 1.4-9.0), those who reported warning messages are important (OR=4.93; 95%CI 1.55-15.71), those who changed their own smoking behavior due the warnings (OR=3.31; 95%CI 1.10-9.99) were more likely to reduce daily number of cigarettes due the health warnings. Forty-seven percent thought that health warnings with text and images could have a higher impact in comparison to text-only warnings. Moreover, women were more motivated to change cigarette brand if a brand should introduce pictorial warnings (OR=2.54; 95%CI 1.41-4.56). CONCLUSION: our study showed some positive effects of the introduction of health warnings on cigarette packages. In fact, almost all were informed on tobacco effects, 14% of them reduced the amount of daily smoking, and 5% attempted to quit. Many smokers still believe that switching to lighter cigarettes (e.g. those with less nicotine and tar contents) reduces smoking-related diseases. More than 50% of smokers recognized the importance of health warnings in communicating health risks of smoking, while women were more impressed than men by shocking pictorial warnings. Descriptors Links Book Title Database Publisher Data Source Authors Mannocci,A., Antici,D., Boccia,A., La Torre,G. Original/Translated Title Impatto delle avvertenze riportate sui pacchetti di sigarette in funzione della dipendenza dal fumo di tabacco e del desiderio di smettere in un campione di fumatori URL Date of Electronic PMCID Editors
A pilot study of StopAdvisor: a theory-based interactive internet-based smoking cessation intervention aimed across the social spectrum	2012	Cancer Research UK Health Behaviour Research Centre, Department of Epidemiology and Public Health, University College London, London, UK. jamie.brown@ucl.ac.uk	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Addictive Behaviors Periodical, Abbrev. Addict.Behav. Pub Date Free Form Dec Volume 37 Issue 12 Start Page 1365 Other Pages 1370 Notes LR: 20141007; CI: Copyright (c) 2012; GR: 14135/Cancer Research UK/United Kingdom; GR: G0802035/Medical Research Council/United Kingdom; JID: 7603486; 2012/03/20 [received]; 2012/05/29 [revised]; 2012/05/30 [accepted]; 2012/06/09 [aheadofprint]; ppublish Place of Publication England ISSN/ISBN 1873-6327; 0306-4603 Accession Number PMID: 22795643 Language eng SubFile Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1016/j.addbeh.2012.05.016 [doi] Output Language Unknown(0) PMID 22795643 Abstract BACKGROUND: This article reports a pilot study of a new smoking cessation website ('StopAdvisor'), which has been developed on the basis of PRIME theory, evidence, web-design expertise and user-testing. The aims were to i) evaluate whether cessation, website usage and satisfaction were sufficiently high to warrant a randomised controlled trial (RCT) and ii) assess whether outcomes were affected by socio-economic status. METHODS: This was an uncontrolled pilot study. Two hundred and four adult daily smokers willing to make a serious quit attempt were included. All participants received support from 'StopAdvisor', which recommends a structured quit plan and a variety of evidence-based behaviour change techniques for smoking cessation. A series of tunnelled sessions and a variety of interactive menus provide tailored support for up to a month before quitting through until one-month post-quit (http://www.lifeguideonline.org/player/play/stopadvisordemonstration). The primary outcome was self-report of at least 1month of continuous abstinence collected at 2months post-enrolment and verified by saliva cotinine or anabasine. Usage was indexed by log-ins and page views. Satisfaction was assessed by dichotomous ratings of helpfulness, personal relevance, likelihood of recommendation and future use, which were collected using an online questionnaire at 2months post-enrolment. Outcomes according to socio-economic status were assessed. RESULTS: At 8weeks post-enrolment, 19.6% (40/204) of participants were abstinent according to the primary outcome criteria (95% C.I.=14.1% to 25.1%). Participants viewed a mean of 133.5 pages (median=71.5) during 6.4 log-ins (median=3). A majority of respondents rated the website positively on each of the four satisfaction `ratings (range=66.7% to 75.3%). There was no evidence of an effect of socio-economic status on abstinence (OR=1.01, C.I.=0.50-2.07), usage (page-views, t(202)=0.11, p=.91; log-ins, t(202)=0.21, p=.83), or satisfaction (helpfulness, OR=1.09, C.I.=0.41-2.88; personal relevance, OR=0.55, C.I.=0.20-1.56; recommendation, OR=0.98, C.I.=0.34-2.81; use in future, OR=1.45, C.I.=0.49-4.27). CONCLUSIONS: The systematic application of theory, evidence, web-design expertise, and user-testing has resulted in a website that shows sufficiently promising efficacy and usability to warrant evaluation in a RCT. The website appears to be similarly effective and acceptable to users across the social spectrum. Descriptors Links Book Title Database Publisher Elsevier Ltd Data Source Authors Brown,J., Michie,S., Geraghty,A.W., Miller,S., Yardley,L., Gardner,B., Shahab,L., Stapleton,J.A., West,R. Original/Translated Title URL Date of Electronic 20120609 PMCID Editors
Prevalence of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in food-producing animals	2012	Department of Microbiology, Shizuoka Institute of Environment and Hygiene, 4-27-2 Kitaando, Aoi, Shizuoka 420-8637, Japan. midori1_hiroi@pref.shizuoka.lg.jp	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full The Journal of veterinary medical science / the Japanese Society of Veterinary Science Periodical, Abbrev. J.Vet.Med.Sci. Pub Date Free Form Feb Volume 74 Issue 2 Start Page 189 Other Pages 195 Notes JID: 9105360; 0 (DNA, Bacterial); EC 3.5.2.- (beta-lactamase CTX-M-14); EC 3.5.2.6 (beta-Lactamases); 2011/10/07 [aheadofprint]; ppublish Place of Publication Japan ISSN/ISBN 1347-7439; 0916-7250 Accession Number PMID: 21979457 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI JST.JSTAGE/jvms/11-0372 [pii] Output Language Unknown(0) PMID 21979457 Abstract To evaluate the diversity of extended-spectrum beta-lactamases (ESBL) genes among food-producing animals, 48 isolates of ESBL-producing Escherichia coli isolates were obtained from rectal samples of broilers, layers, beef cattle and pigs, at the slaughterhouse level. ESBL-carrying E. coli were isolated from 60.0% of individual broiler rectal samples, 5.9% of layers, 12.5% of beef cattle and 3% of pigs. One ESBL-producing Klebsiella pneumoniae was isolated from a broiler. The ESBL-positive E. coli isolates from broilers harbored various ESBL genes: bla (SHV-12), bla(CTX-M-2), bla(CTX-M-14), bla(CTX-M-15) and bla(CTX-M-44). The plasmid DNAs were analyzed by restriction patterns. Homogeneous band patterns were yielded in those of K. pneumoniae and E. coli isolates harboring the bla(CTX-M-2) gene from different farms. No genetic relation between the 2 CTX-M-14 ESBL-producing strains was found by pulsed-field gel electrophoresis, although 2 plasmids in these strains, obtained from different broiler farms, were similar to each other. This study provides evidence that the proliferation of CTX-M-producing E. coli is due to the growth of indigenous CTX-M-producing strains and the possible emergence of strains that acquired CTX-M genes by horizontal transfer in different broiler farms. CTX-M-producing coliforms in broilers should be controlled due to the critical importance of cephalosporins and the zoonotic potential of ESBL-producing bacteria. Descriptors Links Book Title Database Publisher Data Source Authors Hiroi,M., Yamazaki,F., Harada,T., Takahashi,N., Iida,N., Noda,Y., Yagi,M., Nishio,T., Kanda,T., Kawamori,F., Sugiyama,K., Masuda,T., Hara-Kudo,Y., Ohashi,N. Original/Translated Title URL Date of Electronic 20111007 PMCID Editors
Is effective contraceptive use conceived prenatally in Florida? The association between prenatal contraceptive counseling and postpartum contraceptive use	2012	Maternal and Child Heath Practice and Analysis Unit, Division of Family Health Services, Florida Department of Health, Tallahassee, FL, USA. Leticia_hernandez@doh.state.fl.us	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Maternal and child health journal Periodical, Abbrev. Matern.Child Health J. Pub Date Free Form Feb Volume 16 Issue 2 Start Page 423 Other Pages 429 Notes LR: 20151119; GR: 5UR6DP000541-05/DP/NCCDPHP CDC HHS/United States; JID: 9715672; ppublish Place of Publication United States ISSN/ISBN 1573-6628; 1092-7875 Accession Number PMID: 21197562 Language eng SubFile Journal Article; Research Support, U.S. Gov't, P.H.S.; IM DOI 10.1007/s10995-010-0738-9 [doi] Output Language Unknown(0) PMID 21197562 Abstract The objective of this study is to describe the prevalence of postpartum contraceptive use among women experiencing a live birth in Florida in 2004-2005. To examine the association between family planning counseling during prenatal care visits and the use of effective contraceptive methods among postpartum women. Matched data from Florida PRAMS and Vital Statistics were used (n = 3,962). Weighted logistic regression was used to determine the association between prenatal family planning counseling and effective postpartum contraceptive use. Odds ratios were adjusted for use of contraception prior to pregnancy, well-baby checkup, race, ethnicity, nativity, education, marital status, poverty level, stress before or during pregnancy, and parity. Women who reported discussing family planning with their prenatal care provider were more likely to use effective contraception postpartum (AOR: 1.5, 1.1-2.0) compared to women who did not report a discussion. Women who used contraception prior to pregnancy (AOR: 2.3, 1.7-3.2) and women who experienced no stress before or during pregnancy (AOR: 2.0, 1.2-3.4) were also more likely to use contraception in the postpartum period. A significant interaction was identified between family planning counseling during prenatal visits and maternal education. Stratum specific odds ratios for women who received prenatal family planning counseling were significant for women with less than high school education (AOR: 2.5, 1.3-5.1) and for women with high school education (AOR: 2.0, 1.2-3.4). Women reporting family planning counseling during prenatal care were more likely to use effective contraception postpartum. While women with high school or less than high school education levels benefited from prenatal family planning counseling, the greatest benefit was observed for women with less than high school education. Integrating family planning counseling into prenatal care may increase the use of effective contraceptive methods among postpartum women. Descriptors Links Book Title Database Publisher Data Source Authors Hernandez,L.E., Sappenfield,W.M., Goodman,D., Pooler,J. Original/Translated Title URL Date of Electronic PMCID Editors
Culture-based and denaturing gradient gel electrophoresis analysis of the bacterial community from Chungkookjang, a traditional Korean fermented soybean food	2012	Division of Biological Science and Technology, Yonsei University, Wonju, Gangwon 220-710, Republic of Korea.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Journal of Food Science Periodical, Abbrev. J.Food Sci. Pub Date Free Form Oct Volume 77 Issue 10 Start Page M572 Other Pages 8 Notes LR: 20151119; CI: (c) 2012; JID: 0014052; 0 (DNA, Bacterial); 0 (Isoflavones); 0 (RNA, Ribosomal, 16S); 0 (Soybean Proteins); 0 (chungkookjang); 2012/09/05 [aheadofprint]; ppublish Place of Publication United States ISSN/ISBN 1750-3841; 0022-1147 Accession Number PMID: 22950549 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1111/j.1750-3841.2012.02901.x [doi] Output Language Unknown(0) PMID 22950549 Abstract The bacterial community of Chungkookjang and raw rice-straw collected from various areas in South Korea was investigated using both culture-dependent and culture-independent methods. Pure cultures were isolated from Chungkookjang and raw rice-straw on tryptic soy agar plates with 72 to 121 colonies and identified by 16S rDNA gene sequence analysis, respectively. The traditional culture-based method and denaturing gradient gel electrophoresis analysis of PCR-amplified 16S rDNA confirmed that Pantoea agglomerans and B. subtilis were identified as predominant in the raw rice-straw and Chungkookjang, respectively, from Iljuk district of Gyeonggi province, P. ananatis and B. licheniformis were identified as predominant in the raw rice-straw and Chungkookjang from Wonju district of Gangwon province, and Microbacterium sp. and B. licheniformis were identified as predominant in the raw rice-straw and Chungkookjang from Sunchang district of Jeolla province. Other strains, such as Bacillus, Enterococcus, Pseudomonas, Rhodococcus, and uncultured bacteria were also present in raw rice-straw and Chungkookjang. PRACTICAL APPLICATION: A comprehensive analysis of these microorganisms would provide a more detailed understanding of the biologically active components of Chungkookjang and help improve its quality. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis can be successfully applied to a fermented food to detect unculturable or more species than the culture-dependent method. This technique is an effective and convenient culture-independent method for studying the bacterial community in Chungkookjang. In this study, the bacterial community of Chungkookjang collected from various areas in South Korea was investigated using both culture-dependent and culture-independent methods. Descriptors Links Book Title Database Publisher Institute of Food Technologists(R) Data Source Authors Hong,S.W., Choi,J.Y., Chung,K.S. Original/Translated Title URL Date of Electronic 20120905 PMCID Editors

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