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Effect of heavy metals and benzene on porphyrin synthesis of human epithelial larynx carcinoma cells (HEp-2) 2001 Herbarth, O., UFZ - Umweltforsch. Ztrm. L.-H. GmbH, Sekt. Expositionsforsch./Epidemiol., Medizinische Fak. der Univ. Leipzig, D-04318 Leipzig, Germany
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Umweltmedizin in Forschung und Praxis
Periodical, Abbrev.
Umweltmed.Forsch.Prax.
Pub Date Free Form
2001/
Volume
6
Issue
4
Start Page
221
Other Pages
230
Notes
Place of Publication
ISSN/ISBN
1430-8681
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
Aim of the investigation was to prepare an environmental epidemiological test to detect the effects of pollutants by a pattern of porphyrin excretion in the urine. The task was to test whether lead, mercury, copper, zinc and benzene in environmentally relevant concentrations influence porphyrin synthesis of cells. The model used was the permanent epithelial larynx carcinoma cell line HEp-2, which was stimulated to synthesize porphyrins by adding δ-amino-levulinic acid (δ-ALA) in excess. 8- to 4-carboxyporphyrins and protoporphyrin IX were measured both in the cells and in the culture medium by means of high performance liquid cromatography (HPLC). The following pollutant effects were found: lead: extracellular increase of hexacarboxyporphyrin and intracellular decrease of heptacarboxy- and copro-porphyrin; mercury: extracellular decrease of heptacarboxyporphyrin and intracellular increase of coproporphyrin; copper: extracellular decrease of uro-, coproporphyrin and protoporphyrin IX, extracellular increase of heptacarboxyporphyrin and intracellular decrease of uro-, heptacarboxy- and coproporphyrin; zinc: extracellular increase of coproporphyrin; benzene: a tendency to extracellular increase of hexacarboxyporphyrin. The main result is the decrease in the concentrations of porphyrins caused by copper. This result is possibly linked to gastro-intestinal diseases among children due to drinking water from copper water pipes.
Descriptors
aminolevulinic acid, benzene, copper, heavy metal, lead, mercury, porphyrin, protoporphyrin, zinc, article, carcinoma cell, cell culture, culture medium, drinking, gastrointestinal disease, heme synthesis, high performance liquid chromatography, human, larynx carcinoma, pollutant, urinary excretion, water contamination
Links
Book Title
Wirkung von schwermetallen und benzol auf die porphyrinsynthese von humanen epithelialen larynxkarzinomzellen (HEp-2)
Database
Embase
Publisher
Data Source
Embase
Authors
Tumat,C., Kath,H. -G, Perlewitz,J., Herbarth,O., Manuwald,O.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Chlorohydrins of bisphenol A diglycidyl ether (BADGE) and of bisphenol F diglycidyl ether (BFDGE) in canned foods and ready-to-drink coffees from the Japanese market 2001 Tokyo Metropolitan Research Laboratory of Public Health, Japan. uematsu@tokyo-eiken.go.jp
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Food additives and contaminants
Periodical, Abbrev.
Food Addit.Contam.
Pub Date Free Form
Feb
Volume
18
Issue
2
Start Page
177
Other Pages
185
Notes
LR: 20131121; JID: 8500474; 0 (Benzhydryl Compounds); 0 (Carcinogens); 0 (Chlorohydrins); 0 (Coffee); 0 (Epoxy Compounds); 0 (bisphenol F diglycidyl ether); F3XRM1NX4H (2,2-bis(4-glycidyloxyphenyl)propane); ppublish
Place of Publication
England
ISSN/ISBN
0265-203X; 0265-203X
Accession Number
PMID: 11288915
Language
eng
SubFile
Journal Article; IM
DOI
10.1080/02652030010005501 [doi]
Output Language
Unknown(0)
PMID
11288915
Abstract
BADGE.2HCl and BFDGE.2HCl were determined in 28 samples of ready-to-drink canned coffee and 18 samples of canned vegetables (10 corn, 5 tomatoes and 3 others), all from the Japanese market. HPLC was used as the principal analytical method and GC-MS for confirmation of relevant LC fractions. BADGE.2HCl was found to be present in one canned coffee and five samples of corn, BFDGE.2HCl in four samples of canned tomatoes and in one canned corn. No sample was found which exceeded the 1 mg/kg limit of the EU for the BADGE chlorohydrins. However the highest concentration was found for the sum of BFDGE.2HCl anti BFDGE.HCl.H2O at a level of 1.5 mg/kg. A Beilstein test confirmed that all cans containing foods contaminated with BADGE.2HCl or BFDGE.2HCl had at lest one part coated with a PVC organosol.
Descriptors
Benzhydryl Compounds, Carcinogens/analysis, Chlorohydrins/analysis/chemistry, Chromatography, High Pressure Liquid, Coffee/chemistry, Epoxy Compounds/analysis, Food Contamination, Food Packaging, Gas Chromatography-Mass Spectrometry, Humans, Japan, Vegetables/chemistry
Links
Book Title
Database
Publisher
Data Source
Authors
Uematsu,Y., Hirata,K., Suzuki,K., Iida,K., Saito,K.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Smoking affects the subgingival microflora in periodontitis 2001 Department of Oral Biology, Section of Clinical Periodontal Microbiology, Academic Centre for Dentistry, Amsterdam, The Netherlands.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of periodontology
Periodical, Abbrev.
J.Periodontol.
Pub Date Free Form
May
Volume
72
Issue
5
Start Page
666
Other Pages
671
Notes
LR: 20131121; JID: 8000345; ppublish
Place of Publication
United States
ISSN/ISBN
0022-3492; 0022-3492
Accession Number
PMID: 11394403
Language
eng
SubFile
Comparative Study; Journal Article; D; IM
DOI
10.1902/jop.2001.72.5.666 [doi]
Output Language
Unknown(0)
PMID
11394403
Abstract
BACKGROUND: Tobacco smoking has been identified as one major risk factor for destructive periodontal disease. Scaling and root planing have been shown to be less effective in smokers with periodontitis. The aim of the present study was to compare the subgingival microbial flora of treated and untreated smokers and non-smokers. METHODS: Four independent adult patient groups with periodontitis were included in this investigation: 88 untreated smokers (U-S); 90 untreated non-smokers (U-NS); 119 treated non-smokers (T-NS); and 171 treated smokers (T-S). Clinical variables included cumulative plaque index (CPI), probing depth (PD), clinical attachment level (CAL), cumulative bleeding index (CBI), and cumulative suppuration index (CSI). Paper point samples from the deepest bleeding pocket in each quadrant of the dentition were analyzed for the presence and levels of 6 periodontal bacterial pathogens using anaerobic culture techniques. RESULTS: U-S showed a higher mean cumulative plaque index than U-NS (3.5 versus 2.7). Mean PD and mean CAL were higher in the T-S in comparison to the T-NS group (7.0 versus 6.6 mm and 5.6 versus 4.7 mm, respectively). Microbiological characteristics of U-S were a higher prevalence of Prevotella intermedia/nigrescens and higher mean levels of Peptostreptococcus micros (Pm) and Fusobacterium nucleatum (Fn). T-S patients were characterized by higher prevalence of Bacteroides forsythus (Bf), Pm, and Campylobacter rectus (Cr) and higher mean levels of Pm and Fn. The mean percentage of B. forsythus tended to be higher in the T-S group than in the T-NS group (6.9% versus 5.6%). The relative risk to be infected with Bf, Pm, and Cr was statistically higher in smokers (odds ratios: 1.9, 1.9, and 1.6, respectively). The chance to find > or =10% of Bf, Pm, and/or Fn was 3.3 higher in smokers when A. actinomycetemcomitans and P gingivalis were absent. Detection of > or =20% Pm/Fn in treated patients was strongly associated with smoking (odds ratio 13.8, P= 0.002). CONCLUSIONS: Smoking is a determining factor for the composition of the subgingival microflora in adult patients with periodontitis and may select for a specific cluster of periodontal pathogens, notably Bf, Pm, Fn, and Cr. On the basis of these observations, smoking, among other criteria, may be one parameter to use in deciding to treat refractory periodontitis in smokers with a systemic antibiotic therapy directed against smoking-associated periodontal bacteria.
Descriptors
Adult, Aggregatibacter actinomycetemcomitans/classification, Bacteroides/classification, Campylobacter/classification, Chi-Square Distribution, Dental Plaque Index, Fusobacterium nucleatum/classification, Gingiva/microbiology, Gingival Hemorrhage/classification/microbiology, Gram-Negative Bacteria/classification, Humans, Logistic Models, Middle Aged, Odds Ratio, Peptostreptococcus/classification, Periodontal Attachment Loss/classification/microbiology, Periodontal Index, Periodontal Pocket/classification/microbiology, Periodontitis/microbiology/therapy, Porphyromonas gingivalis/classification, Prevotella/classification, Prevotella intermedia/classification, Risk Factors, Smoking/physiopathology, Statistics, Nonparametric
Links
Book Title
Database
Publisher
Data Source
Authors
van Winkelhoff,A. J., Bosch-Tijhof,C. J., Winkel,E. G., van der Reijden,W. A.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis 2001 Institute of Food Technology, University of Hohenheim, Stuttgart, Germany.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Applied and Environmental Microbiology
Periodical, Abbrev.
Appl.Environ.Microbiol.
Pub Date Free Form
Jun
Volume
67
Issue
6
Start Page
2578
Other Pages
2585
Notes
LR: 20140613; JID: 7605801; 0 (DNA Primers); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); OID: NLM: PMC92910; ppublish
Place of Publication
United States
ISSN/ISBN
0099-2240; 0099-2240
Accession Number
PMID: 11375166
Language
eng
SubFile
Journal Article; IM
DOI
10.1128/AEM.67.6.2578-2585.2001 [doi]
Output Language
Unknown(0)
PMID
11375166
Abstract
Denaturing gradient gel electrophoresis (DGGE) of DNA fragments generated by PCR with 16S ribosomal DNA-targeted group-specific primers was used to detect lactic acid bacteria (LAB) of the genera Lactobacillus, Pediococcus, Leuconostoc, and Weissella in human feces. Analysis of fecal samples of four subjects revealed individual profiles of DNA fragments originating not only from species that have been described as intestinal inhabitants but also from characteristically food-associated bacteria such as Lactobacillus sakei, Lactobacillus curvatus, Leuconostoc mesenteroides, and Pediococcus pentosaceus. Comparison of PCR-DGGE results with those of bacteriological culture showed that the food-associated species could not be cultured from the fecal samples by plating on Rogosa agar. On the other hand, all of the LAB species cultured from feces were detected in the DGGE profile. We also detected changes in the types of LAB present in human feces during consumption of a milk product containing the probiotic strain Lactobacillus rhamnosus DR20. The analysis of fecal samples from two subjects taken before, during, and after administration of the probiotic revealed that L. rhamnosus was detectable by PCR-DGGE during the test period in the feces of both subjects, whereas it was detectable by culture in only one of the subjects.
Descriptors
Adult, Clinical Trials as Topic, DNA Primers, DNA, Ribosomal/genetics/isolation & purification, Electrophoresis/methods, Feces/microbiology, Female, Humans, Lactobacillaceae/genetics/isolation & purification, Lactobacillus/isolation & purification, Leuconostoc/isolation & purification, Male, Nucleic Acid Denaturation, Pediococcus/isolation & purification, Polymerase Chain Reaction/methods, Probiotics/pharmacology, RNA, Ribosomal, 16S/genetics/isolation & purification, Streptococcaceae/genetics/isolation & purification
Links
Book Title
Database
Publisher
Data Source
Authors
Walter,J., Hertel,C., Tannock,G. W., Lis,C. M., Munro,K., Hammes,W. P.
Original/Translated Title
URL
Date of Electronic
PMCID
PMC92910
Editors
Comparison of practical treatment methods to eradicate pinworm (Dentostomella translucida) infections from Mongolian gerbils (Meroines unguiculatus). 2001 Wilkerson, J.D., Laboratory Animal Resource Center, University of California, Medical Research Building II, Box 0564, San Francisco, California 94143-0564, USA.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Contemporary topics in laboratory animal science / American Association for Laboratory Animal Science
Periodical, Abbrev.
Contemp.Top.Lab.Anim.Sci.
Pub Date Free Form
/
Volume
40
Issue
5
Start Page
31
Other Pages
36
Notes
Place of Publication
ISSN/ISBN
1060-0558
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
This study evaluates the efficacy of various treatment methods to eradicate Dentostomella translucida from Mongolian gerbil colonies. The following five treatment methods were instituted in naturally infected groups of 10 gerbils each: topical ivermectin misting, ivermectin-medicated drinking water, piperazine citrate-medicated drinking water, fenbendazole-medicated feed, and a combination of ivermectin-medicated drinking water and fenbendazole-medicated feed. Treatment success was assessed by using weekly fecal flotations, with necropsy examinations performed on fecal-negative gerbils (except those in the misted group) at 5 weeks after the last treatment. Topical ivermectin misting left 40% of gerbils fecal-positive. With piperazine citrate-medicated drinking water, 60% of the gerbils were fecal-positive; the remaining 40% had adult worms in their digestive tract at necropsy. Ivermectin-medicated drinking water caused 80% of the gerbils to be negative on fecal flotation. On necropsy, however, all but one of these gerbils harbored adult pinworms. Treatments with fenbendazole-supplemented feed alone or in combination with ivermectin-treated water resulted in no fecal shedding or evidence of adult pinworms on necropsy examination. Of the five treatments evaluated, only those using fenbendazole-medicated feed (150 ppm) provided a practical and reliable treatment method to eradicate pinworm infections in Mongolian gerbil colonies.
Descriptors
antinematodal agent, fenbendazole, ivermectin, piperazine citrate, piperazine derivative, animal, animal disease, animal food, article, comparative study, drinking, drug combination, enterobiasis, experimental animal, feces, female, gerbil, male, parasitology, rodent disease, topical drug administration
Links
Book Title
Database
MEDLINE
Publisher
Data Source
Embase
Authors
Wilkerson,J. D., Brooks,D. L., Derby,M., Griffey,S. M.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Relationship between right-to-left shunts and cutaneous decompression illness 2001 The Royal Shrewsbury Hospital, Shrewsbury SY3 8XQ, UK.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Clinical science (London, England : 1979)
Periodical, Abbrev.
Clin.Sci.(Lond)
Pub Date Free Form
May
Volume
100
Issue
5
Start Page
539
Other Pages
542
Notes
LR: 20041117; JID: 7905731; ppublish
Place of Publication
England
ISSN/ISBN
0143-5221; 0143-5221
Accession Number
PMID: 11294694
Language
eng
SubFile
Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
11294694
Abstract
The presence of a large right-to-left shunt is associated with neurological decompression illness after non-provocative dives, as a result of paradoxical gas embolism. A small number of observations suggest that cutaneous decompression illness is also associated with a right-to-left shunt, although an embolic aetiology of a diffuse rash is more difficult to explain. We performed a retrospective case--control comparison of the prevalence and sizes of right-to-left shunts determined by contrast echocardiography performed blind to history in 60 divers and one caisson worker with a history of cutaneous decompression illness, and 123 historical control divers. We found that 47 (77.0%) of the 61 cases with cutaneous decompression illness had a shunt, compared with 34 (27.6%) of 123 control divers (P<0.001). The size of the shunts in the divers with cutaneous decompression illness was significantly greater than in the controls. Thus 30 (49.2%) of the 61 cases with cutaneous decompression illness had a large shunt at rest, compared with six (4.9%) of the 123 controls (P<0.001). During closure procedures in 17 divers who had cutaneous decompression illness, the mean diameter of the foramen ovale was 10.9 mm. Cutaneous decompression illness occurred after dives that were provocative or deep in subjects without shunts, but after shallower and non-provocative dives in those with shunts. The latter individuals are at increased risk of neurological decompression illness. We conclude that cutaneous decompression illness has two pathophysiological mechanisms. It is usually associated with a large right-to-left shunt, when the mechanism is likely to be paradoxical gas embolism with peripheral amplification when bubble emboli invade tissues supersaturated with nitrogen. Cutaneous decompression illness can also occur in individuals without a shunt. In these subjects, the mechanism might be bubble emboli passing through an 'overloaded' lung filter or autochthonous bubble formation.
Descriptors
Case-Control Studies, Decompression Sickness/etiology, Female, Heart Septal Defects, Atrial/complications/surgery/ultrasonography, Humans, Male, Retrospective Studies, Skin Diseases/etiology, Valsalva Maneuver/physiology
Links
Book Title
Database
Publisher
Data Source
Authors
Wilmshurst,P. T., Pearson,M. J., Walsh,K. P., Morrison,W. L., Bryson,P.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Youth tobacco surveillance--United States, 2000 2001
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
MMWR.CDC surveillance summaries : Morbidity and mortality weekly report.CDC surveillance summaries / Centers for Disease Control
Periodical, Abbrev.
MMWR CDC Surveill.Summ.
Pub Date Free Form
2-Nov
Volume
50
Issue
4
Start Page
1
Other Pages
84
Notes
LR: 20120329; JID: 8407977; 0 (Tobacco Smoke Pollution); EIN: MMWR CDC Surveill Summ 2001 Nov 23;50(46):1036; ppublish
Place of Publication
United States
ISSN/ISBN
Accession Number
PMID: 11902401
Language
eng
SubFile
Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
11902401
Abstract
PROBLEM/CONDITION: Tobacco use is the single leading preventable cause of death in the United States, accounting for approximately 430,000 deaths each year. The prevalence of cigarette smoking nationwide among high school students increased during the 1990s, peaking during 1996-1997, then began a gradual decline. Approximately 80% of tobacco users initiate use before age 18 years. If the trend in early initiation of cigarette smoking continues, approximately 5 million children aged <18 years who are living today will die prematurely because they began to smoke cigarettes during adolescence. The economic costs associated with tobacco use ranges from $53 billion to $73 billion per year in medical expenses and $47 billion in lost productivity. Because of these health and economic consequences, CDC has recommended that states establish and maintain comprehensive tobacco-control programs to reduce tobacco use among youth. REPORTING PERIOD: January 2000 through December 2000. DESCRIPTION OF THE SYSTEM: To assist states in developing and maintaining their state-based comprehensive tobacco prevention and control programs, CDC developed the Youth Tobacco Surveillance and Evaluation System, which includes international, national, and state school-based surveys of middle school and high school students. Two components of this system are discussed in this report--the National Youth Tobacco Survey and the state Youth Tobacco Surveys. The national survey is representative of students in the 50 states and the District of Columbia; 35,828 students in 324 schools completed questionnaires in the spring of 2000. Twenty-nine state surveys were conducted in the spring and fall of 2000; state sample sizes ranged from 583 to 33,586 students. This report summarizes data from the 2000 national survey and state surveys. RESULTS AND INTERPRETATION: Findings from the National Youth Tobacco Survey indicate that current tobacco use ranges from 15.1% among middle school students to 34.5% among high school students. Cigarette smoking is the most prevalent form of tobacco use, followed by cigar smoking and smokeless tobacco use. Approximately one half of current cigarette smokers in middle school and high school report that they usually smoke Marlboro cigarettes. Black students are more likely to smoke Newport cigarettes than any other brand. More than one half of current cigarette smokers in middle school and high school report that they want to stop smoking. Nearly one fourth of middle school and high school students who have never smoked cigarettes are susceptible to initiating cigarette smoking in the next year. Exposure to secondhand smoke (e.g., environmental tobacco smoke) is substantially higher among both middle school and high school students. During the week before the survey, approximately 9 out of 10 current cigarette smokers and one half of never cigarette smokers were in the same room with someone who was smoking cigarettes; and 8 out of 10 current cigarette smokers and 3 out of 10 never cigarette smokers rode in a car with someone who was smoking cigarettes. Approximately 70% of middle school and 57% of high school students who currently smoke cigarettes live in a home where someone smokes cigarettes. Among never cigarette smokers, approximately 3 out of 10 live in a home where someone smokes cigarettes. Approximately 69% of middle school and 58% of high school students aged <18 years who currently smoke cigarettes were not asked to show proof of age when they bought or tried to buy cigarettes. Approximately 8 out of 10 middle school and high school students have seen antismoking commercials. Eight out of 10 middle school students report having seen actors using tobacco on television or in the movies, and approximately 11% of middle school and 16% of high school students who had never used tobacco would wear or use something with a tobacco company name or picture on it. This rate increases to nearly 60% for current tobacco users. PUBLIC
Descriptors
Adolescent, Advertising as Topic, Attitude, Child, Humans, Population Surveillance, Prevalence, Schools, Smoking/epidemiology/prevention & control/psychology, Smoking Cessation, Tobacco Smoke Pollution, United States/epidemiology
Links
Book Title
Database
Publisher
Data Source
Authors
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Hemoglobinuria producida por una fístula en la sutura perivalvular post reemplazo con prótesis valvular mitral 2001
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Periodical, Abbrev.
Acta med.peru
Pub Date Free Form
Volume
18
Issue
1
Start Page
31
Other Pages
34
Notes
ID: 506730
Place of Publication
ISSN/ISBN
Accession Number
Language
es
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
This is a case of 65 year old male with past medical history significant for coronary artery disease and severe mitral valve prolapse for which he underwent simultaneous coronary artery bypass graft using the saphenous vein, mitral valve replacement with a St. Jude prosthetic valve. There were no postoperative complications and after anticoagulation with warfarin the patient wa discharged home. Three months postoperatively the patient developed apparent "hematuria". Physical examination an extensive laboratory analysis of hemoglobinuria. Transthoracic echocardiogram revealed a perivalvular leakage with a normally functioning St. Jude mitral valve replacement. Clinical course was uneventful and the hemoglobinuria resolved spontaneously. This case illustrates the importances in determinig the etiology and pathophysiology of hemoglobinuria with transthoracic and transesophageal echocardiography. (AU)
Descriptors
Humans, Male, Aged, Hemoglobinuria, Heart Valve Prosthesis
Links
http://sisbib.unmsm.edu.pe/BVRevistas/acta_medica/2001_n1/pdf/a06.pdf
Book Title
Database
LILACS; http://www.globalhealthlibrary.net/
Publisher
Data Source
Authors
Agusti,Ré, Yuen,Alberto, Parodi,José
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Longitudinal evaluation of GCF MMP-3 and TIMP-1 levels as prognostic factors for progression of periodontitis 2001 University of the Pacific School of Dentistry, San Francisco, CA 94115, USA. talpagot@uop-edu
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of clinical periodontology
Periodical, Abbrev.
J.Clin.Periodontol.
Pub Date Free Form
Apr
Volume
28
Issue
4
Start Page
353
Other Pages
359
Notes
LR: 20151119; JID: 0425123; 0 (Biomarkers); 0 (Protease Inhibitors); 0 (Tissue Inhibitor of Metalloproteinase-1); EC 3.4.24.17 (Matrix Metalloproteinase 3); ppublish
Place of Publication
Denmark
ISSN/ISBN
0303-6979; 0303-6979
Accession Number
PMID: 11314892
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; D; IM
DOI
Output Language
Unknown(0)
PMID
11314892
Abstract
BACKGROUND: To determine whether matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in gingival crevicular fluid (GCF) could serve as prognostic factors for the progression of periodontitis, we monitored GCF MMP-3 and TIMP-1 and periodontal status of selected sites in 40 medically healthy subjects over a 6-month period. METHOD: Clinical measurements including gingival index (GI), plaque index, bleeding on probing, suppuration, probing depth (PD), attachment loss (AL), and GCF samples were taken from 2 healthy sites (including sites with gingival recession, GI=0 PD or =1; PD > or =5 mm; AL > or =3 mm) of each patient at baseline, 3-month and 6-month visits by means of sterile paper strips. GCF levels of MMP-3 and TIMP-1 were determined by sandwich ELISA assays. RESULTS: The mean amounts of MMP-3 and TIMP-1 in diseased sites were significantly higher than in healthy sites (p<0.0001). Significantly higher GCF levels of MMP-3 and TIMP-1 were found at progressing sites than in nonprogressing periodontitis sites (0.001
or =2 mm loss of attachment during 6- month study period. GCF levels of MMP-3 were highly correlated with clinical measurements taken at baseline, 3-month and 6-month visits (p<0.001). TIMP-1 levels were only moderately correlated with probing depth and attachment level (p<0.01). Step-wise multiple regression analysis was performed to construct models for the prediction of probing depth and attachment loss increases. The most parsimonious regression models which had the best R2 values included the following variables and accounted for the indicated % of variability. The regression model for the prediction of probing depth increase included MMP-3, smoking pack-years, TIMP-1 and accounted for 53% of the variability. The best model for the prediction of attachment loss increase included MMP-3, smoking pack-years, age, TIMP-1 and explained 59% of the variability. CONCLUSION: These data indicate that sites with high GCF levels of MMP-3 and TIMP-1 are at significantly greater risk for progression of periodontitis.
Descriptors
Adult, Age Factors, Analysis of Variance, Biomarkers/analysis, Dental Plaque Index, Disease Progression, Female, Follow-Up Studies, Forecasting, Gingival Crevicular Fluid/enzymology, Gingival Hemorrhage/classification/enzymology, Gingival Recession/enzymology, Gingivitis/enzymology, Humans, Longitudinal Studies, Male, Matrix Metalloproteinase 3/analysis, Middle Aged, Periodontal Attachment Loss/classification/enzymology, Periodontal Index, Periodontal Pocket/classification/enzymology, Periodontitis/enzymology/physiopathology, Prognosis, Protease Inhibitors/analysis, Regression Analysis, Risk Factors, Smoking/physiopathology, Suppuration, Tissue Inhibitor of Metalloproteinase-1/analysis
Links
Book Title
Database
Publisher
Data Source
Authors
Alpagot,T., Bell,C., Lundergan,W., Chambers,D. W., Rudin,R.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Metabolites of a tobacco-specific lung carcinogen in nonsmoking women exposed to environmental tobacco smoke 2001 Division of Epidemiology, University of Minnesota, Minneapolis 55454, USA. anderson_k@epi.umn.edu
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of the National Cancer Institute
Periodical, Abbrev.
J.Natl.Cancer Inst.
Pub Date Free Form
7-Mar
Volume
93
Issue
5
Start Page
378
Other Pages
381
Notes
LR: 20151119; GR: CA81301/CA/NCI NIH HHS/United States; JID: 7503089; 0 (4-((methylnitrosoamino)-1-(3-pyridyl)but-1-yl)beta-omega-glucosiduronic acid); 0 (Carcinogens); 0 (Glucuronates); 0 (Nitrosamines); 0 (Tobacco Smoke Pollution); 6M3C89ZY6R (Nicotine)
Place of Publication
United States
ISSN/ISBN
0027-8874; 0027-8874
Accession Number
PMID: 11238699
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; IM
DOI
Output Language
Unknown(0)
PMID
11238699
Abstract
BACKGROUND: Environmental tobacco smoke (ETS) is associated with lung cancer in nonsmokers. Most epidemiologic studies find a higher risk for lung cancer in nonsmoking women married to smokers than in those married to nonsmokers. We measured metabolites of a tobacco-specific lung carcinogen in urine from healthy, nonsmoking women exposed to ETS. METHODS: We recruited women and their partners through advertisements. Couples completed questionnaires on smoking history and demographics, and both partners provided 100 mL of urine; 23 women had male partners who smoked in the home (i.e., exposed women), and 22 women had male partners who did not smoke (i.e., unexposed women). Urine samples were analyzed for nicotine, for cotinine, for 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and its glucuronide (NNAL-Gluc), as well as for creatinine. NNAL and NNAL-Gluc are metabolites of the tobacco-specific lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Unpaired Student's t tests were conducted on log-transformed values. All statistical tests are two-sided. RESULTS: Urinary levels of nicotine, cotinine, NNAL, and NNAL-Gluc were statistically significantly higher in exposed women than in unexposed women. Geometric means for these compounds in exposed versus unexposed women, respectively, were as follows: nicotine, 0.050 nmol/mg of creatinine (95% confidence interval [CI] = 0.033 to 0.076) versus 0.008 nmol/mg of creatinine (95% CI = 0.004 to 0.014); cotinine, 0.037 nmol/mg of creatinine (95% CI = 0.022 to 0.061) versus 0.007 nmol/mg of creatinine (95% CI = 0.004 to 0.011); NNAL, 0.013 pmol/mg of creatinine (95% CI = 0.007 to 0.024) versus 0.004 pmol/mg of creatinine (95% CI = 0.002 to 0.007); and NNAL-Gluc, 0.027 pmol/mg of creatinine (95% CI = 0.016 to 0.045) versus 0.004 pmol/mg of creatinine (95% CI = 0.003 to 0.006). CONCLUSIONS: Nonsmoking women exposed to ETS take up and metabolize the tobacco-specific lung carcinogen NNK, which could increase their risk of lung cancer. Within couples, the NNAL plus NNAL-Gluc level in exposed women compared with that of their smoking partners averaged 5.6%. Notably, epidemiologic studies have estimated the excess risk for lung cancer in nonsmoking women exposed to ETS as 1%-2% of that in smokers.
Descriptors
Adult, Aged, Carcinogens/adverse effects/metabolism, Cotinine/urine, Creatinine/urine, Female, Glucuronates/urine, Humans, Lung Neoplasms/etiology/metabolism/urine, Middle Aged, Nicotine/urine, Nitrosamines/urine, Risk, Risk Factors, Smoking/adverse effects, Surveys and Questionnaires, Tobacco Smoke Pollution/adverse effects
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Anderson,K. E., Carmella,S. G., Ye,M., Bliss,R. L., Le,C., Murphy,L., Hecht,S. S.
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