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The potential of a polyphasic PCR-dGGE approach in evaluating microbial diversity of natural whey cultures for water-buffalo Mozzarella cheese production: bias of culture-dependent and culture-independent analyses 2001 Dipartimento di Scienza degli Alimenti, Alimentare e Ambientale e di Igiene, Stazione di Microbiologia Industriale, Universita degli Studi di Napoli Federico II, Portici, Italy.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Systematic and applied microbiology
Periodical, Abbrev.
Syst.Appl.Microbiol.
Pub Date Free Form
Dec
Volume
24
Issue
4
Start Page
610
Other Pages
617
Notes
LR: 20061115; JID: 8306133; 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); ppublish
Place of Publication
Germany
ISSN/ISBN
0723-2020; 0723-2020
Accession Number
PMID: 11876368
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
S0723-2020(04)70075-2 [pii]
Output Language
Unknown(0)
PMID
11876368
Abstract
A polyphasic PCR-DGGE approach was used to describe the microbial population occurring in natural whey cultures (NWCs) for water-buffalo Mozzarella cheese production. Total microbial community was assessed without cultivation by analyzing DNA directly extracted from the original samples of NWC. In addition, DNA extracted from bulks of cells formed by harvesting colonies from the serial dilution agar plates of a variety of culture media was used to profile the "cultivable" community. The 16S rDNA V3 region was amplified using DNA from NWC as well as DNA from bulks as templates and the amplicons were separated by DGGE. The microbial entities occurring in NWCs were identified by partial 16S rDNA sequencing of DGGE bands: four lactic acid bacteria (LAB) closest relative of Streptococcus thermophilus, Lactococcus lactis, Lactobacillus delbrueckii and Lactobacillus crispatus were revealed by the analysis of DNA directly extracted from NWC while two other LAB, Lactobacillus fermentum and Enterococcus faecalis, were identified by analyzing DNA from the cultivable community. The developed PCR-DGGE analysis of the "cultivable" community showed good potential in evaluating microbial diversity of a dairy environment: it usefully highlighted the bias introduced by selective amplification when compared to the analysis of the total community from NWC and allowed suitability of media and growth conditions to be evaluated. Moreover, it could be used to complete the culture independent study of microbial diversity to give information on concentration ratios among species occurring in a particular environment and can be proposed for rapid identification of dominant microorganisms in alternative to traditional tools.
Descriptors
Animals, Base Sequence, Buffaloes, Cheese/microbiology, Colony Count, Microbial, DNA, Bacterial/chemistry/genetics, Electrophoresis, Polyacrylamide Gel, Enterococcus faecalis/genetics/isolation & purification, Food Microbiology, Italy, Lactobacillus/genetics/isolation & purification, Molecular Sequence Data, Polymerase Chain Reaction, RNA, Ribosomal, 16S/chemistry/genetics, Sequence Analysis, DNA, Streptococcus/genetics/isolation & purification
Links
Book Title
Database
Publisher
Data Source
Authors
Ercolini,D., Moschetti,G., Blaiotta,G., Coppola,S.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Long-term monitoring of atmospheric deposition and the implications of ionic inputs for the sustainability of a coniferous forest ecosystem 2001 Farrell, E.P., Forest Ecosystem Research Group, Faculty of Agriculture, University College Dublin, Dublin 4, Ireland
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Water, air, and soil pollution
Periodical, Abbrev.
Water Air Soil Pollut.
Pub Date Free Form
2001/
Volume
130
Issue
1-4 III
Start Page
1055
Other Pages
1060
Notes
Place of Publication
ISSN/ISBN
0049-6979
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
Ionic fluxes in a semi-mature stand of Sitka spruce (Picea sitchensis (Bong.) Carr.), on a spodosol in eastern Ireland, were monitored over an eight-year period, 1991-1998. The paper focuses on the long-term viability of forests in this region. Input-output balances, proton budgets and critical loads suggest that the long-term sustainability of forests in the region is threatened unless atmospheric emissions of anthropogenic substances can be controlled.
Descriptors
ion, proton, article, atmospheric transport, controlled study, ecosystem, environmental management, environmental monitoring, environmental protection, forest, ion transport, Ireland, nonhuman, plant
Links
Book Title
Database
Embase
Publisher
Data Source
Embase
Authors
Farrell,E. P., Aherne,J., Boyle,G. M., Nunan,N.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Problems of release of solid asphalt particles into drinking water 2001 Fiedorová, M., Okresni Hygienicka Stanice, 734 01 Karvina, Czech Republic
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Hygiena
Periodical, Abbrev.
Hygiena
Pub Date Free Form
2001/
Volume
46
Issue
1
Start Page
33
Other Pages
36
Notes
Place of Publication
ISSN/ISBN
1210-7840
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
The authors draw attention to the release of parts of anti-corrosive linings of water pipes with an asphalt base into drinking water. Fragments in the water pipe system are gradually crushed and the smallest particles penetrate as far as the consumer. Polyaromatic hydrocarbons are extracted. On an actual example the authors describe in detail the procedure used by the district hygiene officer in solving this problem.
Descriptors
asphalt, drinking water, polycyclic aromatic hydrocarbon, article, corrosion, hygiene, particulate matter, pipeline, problem solving, water quality
Links
Book Title
Problematika uvolňování pevných částic asfaltové povahy do pitné vody
Database
Embase
Publisher
Data Source
Embase
Authors
Fiedorová,M., Anděl,K.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Quantitative ultrasound and bone mineral density are equally strongly associated with risk factors for osteoporosis 2001 Osteoporosis Screening and Research Unit, Guy's Hospital, London, United Kingdom.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Periodical, Abbrev.
J.Bone Miner.Res.
Pub Date Free Form
Feb
Volume
16
Issue
2
Start Page
406
Other Pages
416
Notes
LR: 20071115; JID: 8610640; ppublish
Place of Publication
United States
ISSN/ISBN
0884-0431; 0884-0431
Accession Number
PMID: 11204441
Language
eng
SubFile
Journal Article; IM
DOI
10.1359/jbmr.2001.16.2.406 [doi]
Output Language
Unknown(0)
PMID
11204441
Abstract
Because resources do not allow all women to be screened for osteoporosis, clinical risk factors are often used to identify those individuals at increased risk of fracture who are then assessed by bone densitometry. The aim of this study was to compare calcaneal quantitative ultrasound (QUS) and axial bone mineral density (BMD) T and Z scores in a large group of women, some with no clinical risk factors and others with one or more risk factors for osteoporosis. The study population consisted of 1115 pre- and postmenopausal women. A subgroup of 530 women was used to construct reference data for calculating T and Z scores. A total of 786 women was found to have one or more of the following risk factors: (i) atraumatic fracture since the age of 25 years, (ii) report of X-ray osteopenia, (iii) predisposing medical condition or use of therapy known to affect bone metabolism, (iv) premature menopause before the age of 45 years or a history of amenorrhea of longer than 6 months duration, (v) family history of osteoporosis, (vi) body mass index (BMI) <20 kg/m2, and (vii) current smoking habit. Calcaneal broadband ultrasound attenuation (BUA) and speed of sound (SOS) measurements were performed on a Hologic Sahara and a DTUone and BMD was measured at the spine and hip using dual-energy X-ray absorptiometry (DXA). The Z score decrements associated with the seven risk factors calculated using multivariate regression analysis were similar for QUS and BMD measurements. Z score decrements (mean of BMD and QUS measurements combined) associated with a history of atraumatic fracture (-0.67), X-ray osteopenia (-0.36), a family history of osteoporosis (-0.23), and a low BMI (-0.53) were all statistically significant compared with women with no risk factors. Z score decrements associated with a medical condition or use of therapy known to affect bone metabolism, a premature menopause or prolonged amenorrhea, or those who were current smokers were not significantly different from zero. As the number of risk factors present in each individual increased, the mean Z score decrements became more negative, increasing from -0.28 for women with one risk factor to -1.19 for those with four or more risk factors. QUS and BMD measurements yielded similar mean Z scores for women with one, two, three, or more than four risk factors. Using the World Health Organization (WHO) criteria to diagnose osteoporosis for BMD measurements and revised diagnostic criteria for QUS, approximately one-third of postmenopausal women aged 50+ years with clinical risk factors were classified as osteoporotic compared with only 12% of women without clinical risk factors. Over two-thirds of postmenopausal women with risk factors were classified as osteopenic or osteoporotic and approximately 28% were classified as normal. The proportion of women classified into each diagnostic category was similar for BMD and QUS. In conclusion, clinical risk factors for osteoporosis affected calcaneal BUA and SOS Z score measurements to the same extent as axial BMD Z score measurements. Provided revised diagnostic criteria are adopted for QUS, similar proportions of postmenopausal women are identified as osteopenic or osteoporotic as with BMD.
Descriptors
Absorptiometry, Photon, Adult, Bone Density, Humans, Male, Middle Aged, Osteoporosis/physiopathology/ultrasonography, Prevalence, Risk Factors
Links
Book Title
Database
Publisher
Data Source
Authors
Frost,M. L., Blake,G. M., Fogelman,I.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Effects of maternal smoking during pregnancy and environmental tobacco smoke on asthma and wheezing in children 2001 Keck School of Medicine, Department of Preventive Medicine, University of Southern California, Los Angeles, California 90033, USA. gillilland@hsc.usc.edu
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
American journal of respiratory and critical care medicine
Periodical, Abbrev.
Am.J.Respir.Crit.Care Med.
Pub Date Free Form
Feb
Volume
163
Issue
2
Start Page
429
Other Pages
436
Notes
LR: 20071114; GR: 1R01 HL61768-01/HL/NHLBI NIH HHS/United States; GR: 5P01 ES09581/ES/NIEHS NIH HHS/United States; GR: 5P30 ES07048-02/ES/NIEHS NIH HHS/United States; GR: R826708-01/PHS HHS/United States; JID: 9421642; 0 (Tobacco Smoke Pollution); ppublis
Place of Publication
United States
ISSN/ISBN
1073-449X; 1073-449X
Accession Number
PMID: 11179118
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; AIM; IM
DOI
10.1164/ajrccm.163.2.2006009 [doi]
Output Language
Unknown(0)
PMID
11179118
Abstract
The effects of maternal smoking during pregnancy and childhood environmental tobacco smoke (ETS) exposure on asthma and wheezing were investigated in 5,762 school-aged children residing in 12 Southern California communities. Responses to a self- administered questionnaire completed by parents of 4th, 7th, and 10th grade students were used to ascertain children with wheezing or physician-diagnosed asthma. Lifetime household exposures to tobacco smoke were assessed using responses about past and current smoking histories of household members and any history of maternal smoking during pregnancy. Logistic regression models were fitted to cross-sectional data to estimate the effects of in utero exposure to maternal smoking and previous and current ETS exposure on the prevalence of wheezing and physician-diagnosed asthma. In utero exposure to maternal smoking without subsequent postnatal ETS exposure was associated with increased prevalence of physician-diagnosed asthma (OR, 1.8; 95% CI, 1.1 to 2.9), asthma with current symptoms (OR, 2.3; 95% CI, 1.3 to 4.0), asthma requiring medication use in the previous 12 mo (OR, 2.1; 95% CI, 1.2 to 3.6), lifetime history of wheezing (OR, 1.8; 95% CI, 1.2 to 2.6), current wheezing with colds (OR, 2.1; 95% CI, 1.3 to 3.4) and without colds (OR, 2.5; 95% CI, 1.4 to 4.4), persistent wheezing (OR, 3.1; 95% CI, 1.6 to 6.1), wheezing with exercise (OR, 2.4; 95% CI; 1.3 to 4.3), attacks of wheezing causing shortness of breath (OR, 2.4; 95% CI, 1.3 to 4.4) or awakening at night in the previous 12 mo (OR, 3.2; 95% CI, 1.7 to 5.8), and wheezing requiring medication (OR, 2.1; 95% CI, 1.2 to 3.7) or emergency room visits during the previous year (OR, 3.4; 95% CI, 1.4 to 7.8). In contrast, current and previous ETS exposure was not associated with asthma prevalence, but was consistently associated with subcategories of wheezing. Current ETS exposure was associated with lifetime wheezing (OR, 1.3; 95% CI, 1.1 to 1.5), current wheezing with colds (OR, 1.6; 95% CI, 1.3 to 2.0) and without colds (OR, 1.5; 95% CI, 1.1 to 1.9), wheezing with exercise (OR, 1.7; 95% CI, 1.3 to 2.2), attacks of wheezing causing shortness of breath (OR, 1.6; 95% CI, 1.2 to 2.1) or awakening at night (OR, 1.5; 95% CI, 1.1 to 2.0), and wheezing requiring medication (OR, 1.4; 95% CI, 1.1 to 1.8) or emergency room visits within the previous year (OR, 1.9; 95% CI, 1.2 to 3.0). The effects of current ETS exposure on subcategories of wheezing were most pronounced among children exposed to two or more smokers and remained significant after adjusting for maternal smoking during pregnancy. We conclude that maternal smoking during pregnancy increases the occurrence of physician-diagnosed asthma and wheezing during childhood. In contrast, current ETS exposure is associated with wheezing, but not physician-diagnosed asthma. Taken together, our findings support the hypothesis that ETS operates as a cofactor with other insults such as intercurrent infections as a trigger of wheezing attacks, rather than as a factor that induces asthma, whereas in utero exposure acts to increase physician-diagnosed asthma
Descriptors
Adolescent, Asthma/etiology, California, Child, Child, Preschool, Female, Humans, Infant, Infant, Newborn, Male, Pregnancy, Respiratory Sounds/etiology, Risk Factors, Smoking/adverse effects, Tobacco Smoke Pollution/adverse effects
Links
Book Title
Database
Publisher
Data Source
Authors
Gilliland,F. D., Li,Y. F., Peters,J. M.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Cement-based materials used in drinking water pipe systems 2001 Soukatchoff, P., Saint-Gobain PAM, 54076 Nancy Cedex, France
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Water Science and Technology: Water Supply
Periodical, Abbrev.
Water Sc.Technol.Water Supply
Pub Date Free Form
2001/
Volume
1
Issue
4
Start Page
227
Other Pages
236
Notes
Place of Publication
ISSN/ISBN
1606-9749
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
Ductile iron pipes with cement mortar linings and concrete pipes are used with satisfaction for the transport and distribution of drinking water. The cement-based materials involved in these pipes are very well accepted, being considered as "traditional" and well proven for this application. An interaction with water however exists and two types of questions can be raised: the durability of the material over long periods (more than 50 years) and the preservation of water quality. These two aspects have been deeply investigated in the last 10 years by several European laboratories co-operating with organizations like CEN (European Standardization Committee), CEOCOR, AGHTM, etc. This paper gives an overview of the work which has been done focusing especially on a survey of the behaviour of mortars, made from different type of cements, exposed for more that 15 years to a very soft acidic water the models for calculating the calco-carbonic equilibrium and the main results of a European co-normative research programme carried out to provide information to allow the development of test methods to assess the migration of mineral and organic substances from cement-based materials in contact with drinking water.
Descriptors
carbon, cement, drinking water, analytic method, conference paper, materials handling, tube, water quality, water supply, water transport
Links
Book Title
Database
Embase
Publisher
Data Source
Embase
Authors
Soukatchoff,P., Baron,J., Lessirard,L.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Epidemiology and laboratory diagnostics of legionellae 2001 Szénási, Z., Szegedi Tudományegyetem, Szent-Györgyi Albert Orvos- és Gyógyszerésztudományi Centrum, Altalános Orvostudományi Kar, Klinikai Mikrobiológiai Diagnosztikai Intézet.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Orvosi hetilap
Periodical, Abbrev.
Orv.Hetil.
Pub Date Free Form
/
Volume
142
Issue
20
Start Page
1035
Other Pages
1043
Notes
Place of Publication
ISSN/ISBN
0030-6002
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
The severe pulmonary disease caused by the inhalation of the different Legionella species is called Legionella pneumonia, while the name of the pulmonary disease caused by the most common Legionella (L. pneumophila) is Legionnaires' disease. Another type of disease caused by legionellae is Pontiac fever with influenza-like symptoms. Legionella spp. are facultative intracellular parasites. They survive within both monocytes in the human organism and amebae in the environment. To prevent and control the occurrence of legionelloses, legionellae should be surveyed and detected in the environmental (water pipes, air-conditioning systems, cooling towers, respiratory equipments, etc.) and clinical (blood, bronchoalveolar lavage, sputum, abscess, etc.) samples. Laboratory diagnosis is complicated by the limitations of the available assays. Thus, it is proposed that the microbiological laboratory diagnosis should be based on the simultaneous application of at least three methods (culturing [on BCYE medium], followed by biochemical assays, serology, molecular biologic methods, such as polymerase chain reaction [PCR], direct demonstration [immunofluorescence microscopy], antigen determination are the most important ones) and on the simultaneous demonstration from three different samples (e.g. lower respiratory tract secretions, sputum, urine, blood culture, serum, moreover, water samples from all potential infectious sources, sediment of hot water tanks, as well as swab samples of faucets and shower heads). The advantage of PCR is that is gives reliable results in one day, in contrast to conventional culturing. However, its sensitivity can not be improved by increasing the sample volume, and neither can it give quantitative results nor can it produce strains for epidemiologic studies, contrary to the method of culturing. It is concluded that PCR and culturing do complement, but do not substitute each other.
Descriptors
bacterial antigen, bacterial DNA, cell culture, diagnostic procedure, genetics, human, immunology, isolation and purification, Legionella, Legionella pneumophila, legionnaire disease, microbiology, polymerase chain reaction, review, standard
Links
Book Title
A legionellák epidemiológiája és laboratóriumi diagnosztikája.
Database
MEDLINE
Publisher
Data Source
Embase
Authors
Szénási,Z., Endo,T., Yagita,K., Veréb,I., Nagy,E.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Early invasive pulmonary aspergillosis in a leukemia patient linked to aspergillus contaminated marijuana smoking 2001 Lahav, M., Medicine E Sapir Medical Center, Meir Hospital, Tel-Aviv University, Kfar Saba, Israel
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Leukemia and Lymphoma
Periodical, Abbrev.
Leuk.Lymphoma
Pub Date Free Form
2001/
Volume
42
Issue
6
Start Page
1433
Other Pages
1437
Notes
Place of Publication
ISSN/ISBN
1042-8194
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
46-year-old patient with acute myeloid leukemia (AML) whose disease manifested as fever, chills and dry cough is reported here. Despite broad antibiotic coverage he remained acutely ill with spiking fever, shaking chills, and hypoxemia. His initial chest radiograph was normal but chest computed tomography (CT) scan disclosed bilateral focal infiltrates. Hypoxemia and severe thrombocytopenia precluded invasive diagnostic procedures. A thorough epidemiological investigation revealed that before becoming acutely ill the patient smoked daily tobacco mixed with marijuana from a "hookah bottle". While waiting for tobacco and "hookah water" cultures, we started antifungal therapy. Resolution of fever and hypoxemia ensued after 72 hours. Tobacco cultures yielded heavy growth of Aspergillus species. We suggest that habitual smoking of Aspergillus-infested tobacco and marijuana caused airway colonization with Aspergillus. Leukemia rendered the patient immunocompromised, and allowed Aspergillus to infest the lung parenchyma with early occurrence of invasive pulmonary aspergillosis. Physicians should be aware of this potentially lethal complication of "hookah" and marijuana smoking in immunocompromised hosts.
Descriptors
amphotericin B, antibiotic agent, antifungal agent, cytarabine, etoposide, idarubicin, acute disease, acute myeloblastic leukemia, adult, antibiotic therapy, article, Aspergillus, cannabis addiction, case report, chill, computer assisted tomography, coughing, diagnostic procedure, disease severity, drug megadose, fever, fungal contamination, fungus culture, human, hypoxemia, immune deficiency, lung aspergillosis, lung infiltrate, lung parenchyma, male, priority journal, respiratory tract infection, smoking, thorax radiography, thrombocytopenia, tobacco, treatment failure
Links
Book Title
Database
Embase; MEDLINE
Publisher
Data Source
Embase
Authors
Szyper-Kravitz,M., Lang,R., Manor,Y., Lahav,M.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Reduced spinal bone mineral density in adolescents of an Ultra-Orthodox Jewish community in Brooklyn 2001 Division of Pediatric Endocrinology, Maimonides Medical Center, Brooklyn, New York 11219, USA.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Pediatrics
Periodical, Abbrev.
Pediatrics
Pub Date Free Form
May
Volume
107
Issue
5
Start Page
E79
Other Pages
Notes
LR: 20071115; JID: 0376422; ppublish
Place of Publication
United States
ISSN/ISBN
1098-4275; 0031-4005
Accession Number
PMID: 11331729
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; AIM; IM
DOI
Output Language
Unknown(0)
PMID
11331729
Abstract
OBJECTIVES: Bone mass increases throughout childhood, with maximal bone mass accrual rate occurring in early to mid-puberty and slowing in late puberty. Prevention of osteoporosis and its morbidities depends primarily on the establishment of adequate peak bone mass. Physical activity, calcium intake, and vitamin D stores (from sunlight conversion of precursors of vitamin D and to a lesser degree from dietary intake) are vital determinants of bone mineral density (BMD). BMD is further controlled by genetic and environmental factors that are poorly understood. Observance of ultra-Orthodox Jewish customs may have a negative effect on the factors that promote bone health, and there have been anecdotal reports of higher fracture rates in this population. The ultra-Orthodox Jewish lifestyle encourages scholarly activity in preference to physical activity. Additionally, modest dress codes and inner-city dwelling reduce sunlight exposure. Orthodox Jews do not consume milk products for 6 hours after meat ingestion, leading to potentially fewer opportunities to consume calcium. Foods from the milk group are some of the best sources of dietary calcium. Our aims are to examine BMD in a group of healthy ultra-Orthodox Jewish adolescents in an urban community and to attempt to correlate it to physical activity and dietary factors. DESIGN AND METHODS: We recruited 50 healthy, ultra-Orthodox Jews, ages 15 to 19 years (30 males and 20 females). None were taking corticosteroids or had evidence of malabsorption. All girls were postmenarchal and nulliparous. Pubic hair Tanner stage for boys and breast Tanner stage for girls were determined. Weight and height standard deviation scores were calculated. Calcium, phosphorus, protein, vitamin D, and calorie intake were assessed using a comprehensive food questionnaire referring to what has been eaten over the last year. Hours per week of weight-bearing exercise and walking were determined. Serum levels of calcium, intact parathyroid hormone (PTH), 25 hydroxyvitamin D (25[OH]D) and 1,25 dihydroxyvitamin D (1,25[OH](2)D) were measured. Lumbar spine (L) BMD was assessed by dual energy radiograph absorptiometry. The pediatric software supplied by Lunar Radiation Corporation, which contains gender- and age-specific norms, provided a z score for the lumbar BMD for each participant. L2 to L4 bone mineral apparent density (BMAD) was calculated from L2 to L4 BMD. RESULTS: BMD of L2 to L4 was significantly decreased compared with age/sex-matched normative data: mean z score was -1.25 +/- 1.25 (n = 50). The mean L2 to L4 BMD z score +/- standard deviation was -1.71 +/- 1.18 for boys and -0.58 +/- 1.04 for girls. Eight boys (27%) had L2 to L4 BMD z scores <-2.5, which defines osteoporosis in adulthood. Twenty-seven adolescents (54%), 16 boys and 11 girls, had Tanner stage V. Two participants (4%) had delayed development of Tanner stage V. Mean consumption of calcium by participants under 19 years old was 908 +/- 506 mg/day (n = 46), which is lower than the adequate intake of 1300 mg/day for this age. The consumption of phosphorus was 1329 +/- 606 mg/day, and the consumption of vitamin D was 286 +/- 173 IU/day (n = 50). The mean serum 25(OH)D level was 18.4 +/- 7.6 ng/mL, and the mean serum 1,25(OH)(2)D level was 71.1 +/- 15.7 pg/mL (n = 50). Boys had significantly higher serum levels of 1,25(OH)(2)D than did girls (74.9 +/- 16.46 pg/mL vs 65.25 +/- 12.8 pg/mL, respectively). The serum levels of PTH, calcium, and protein were (mean +/- standard deviation): 33 +/- 16 pg/mL, 9.5 +/- 0.69 mg/dL, and 7.8 +/- 0.6 g/dL, respectively (n = 50). L2 to L4 BMD z score had positive correlation with walking hours (r = 0.4). L2 to L4 BMD z score had negative correlation with serum level of 1,25(OH)(2)D )r = -0.33; n = 50). We could not find significant correlation between L2 to L4 BMD z scores for the entire cohort and any of calcium, vitamin D, phosphorus, or protein intake. However, the L2 to L4 BMD z scores of boys had po
Descriptors
Absorptiometry, Photon, Adolescent, Bone Density, Cross-Sectional Studies, Diet, Exercise, Female, Humans, Jews, Judaism, Lumbar Vertebrae/radiography, Male, New York, Regression Analysis, Sunlight
Links
Book Title
Database
Publisher
Data Source
Authors
Taha,W., Chin,D., Silverberg,A. I., Lashiker,L., Khateeb,N., Anhalt,H.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Untreated periodontal disease in Indonesian adolescents. Subgingival microbiota in relation to experienced progression of periodontitis 2001 Department of Periodontology, Academic Centre for Dentistry Amsterdam, ACTA, The Netherlands. M.Timmerman@acta.nl
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of clinical periodontology
Periodical, Abbrev.
J.Clin.Periodontol.
Pub Date Free Form
Jul
Volume
28
Issue
7
Start Page
617
Other Pages
627
Notes
LR: 20131121; JID: 0425123; ppublish
Place of Publication
Denmark
ISSN/ISBN
0303-6979; 0303-6979
Accession Number
PMID: 11422582
Language
eng
SubFile
Journal Article; D; IM
DOI
cpe280703 [pii]
Output Language
Unknown(0)
PMID
11422582
Abstract
BACKGROUND/AIMS: In an Indonesian population deprived of regular dental care, the experienced progression of disease between baseline (1987) and follow-up (1994) was investigated in relation to the composition of the subgingival microbiota at follow-up. At baseline the age ranged from 15 to 25 years. Clinical and microbiological evaluation was completed in 158 of the 167 subjects available at follow-up. METHODS: Plaque index (PI), pocket depth (PD), bleeding on probing (BOP), and attachment loss (AL) were scored at the approximal surfaces of all teeth and subgingival calculus on the approximal surfaces of the Ramfjord teeth only (number of sites with subgingival calculus: NSC). A pooled sample of the deepest pocket in each quadrant was evaluated using microbiological culture techniques. RESULTS: At baseline the mean values of the clinical parameters were AL=0.35 mm, PI=1.01, BOP=0.80 PD=3.25 mm and NSC=6.04 and at follow-up AL=0.75 mm, PI=1.16, BOP=1.19, PD=3.34 mm and NSC=5.85. All parameters except PD and NSC showed a statistically significant increase. At follow-up the prevalence of Actinobacillus actinomycetemcomitans was 40%, of Porphyromonas gingivalis 67%, of Prevotella intermedia 66%, of Fusobacterium nucleatum 79%, of Bacteroides forsythus 16%, of Campylobacter rectus 4%, and of P. micros 6%. No differences in clinical parameters were found between groups with or without these micro-organisms. In 129 subjects AL of > or =2 mm at > or =1 site was found. Logistic regression showed three significant odds-ratio's for experienced progressive periodontitis: Plaque index (12.2), gender (3.4) and Actinobacillus actinomycetemcomitans (2.9). CONCLUSIONS: The results of this retrospective study suggest that plaque is the most important parameter related to experienced disease progression, and that the presence of A. actinomycetemcomitans may be associated with increased chance of disease progression.
Descriptors
Adolescent, Adult, Aggregatibacter actinomycetemcomitans/growth & development, Bacteria/classification, Bacteroides/growth & development, Campylobacter/growth & development, Confidence Intervals, Dental Calculus/classification, Dental Plaque Index, Disease Progression, Female, Follow-Up Studies, Fusobacterium nucleatum/growth & development, Gingiva/microbiology, Gingival Hemorrhage/classification/microbiology, Humans, Indonesia, Logistic Models, Male, Odds Ratio, Peptostreptococcus/growth & development, Periodontal Attachment Loss/classification/microbiology, Periodontal Diseases/microbiology/physiopathology, Periodontal Index, Periodontal Pocket/classification/microbiology, Periodontitis/microbiology/physiopathology, Porphyromonas gingivalis/growth & development, Prevotella intermedia/growth & development, Retrospective Studies, Sex Factors
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Timmerman,M. F., Van der Weijden,G. A., Arief,E. M., Armand,S., Abbas,F., Winkel,E. G., Van Winkelhoff,A. J., Van der Velden,U.
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