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Consumption of tobacco, alcohol and drugs among adolescents in Germany. Results of the German Health Interview and Examination Survey for Children and Adolescents (KiGGS) 2007 Robert Koch-Institut, Berlin, BRD. t.lampert@rki.de
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz
Periodical, Abbrev.
Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz
Pub Date Free Form
May-Jun
Volume
50
Issue
6-May
Start Page
600
Other Pages
608
Notes
LR: 20160707; JID: 101181368; 0 (Tobacco Smoke Pollution); ppublish
Place of Publication
Germany
ISSN/ISBN
1436-9990; 1436-9990
Accession Number
PMID: 17514444
Language
ger
SubFile
English Abstract; Journal Article; IM
DOI
10.1007/s00103-007-0221-y [doi]
Output Language
Unknown(0)
PMID
17514444
Abstract
Due to its long-lasting effects, the consumption of tobacco, alcohol and drugs is one of the central topics of prevention and health promotion in childhood and adolescence. The data from the German Health Interview and Examination Survey for Children and Adolescents (KiGGS) show that in Germany today 20.5 % of 11-17-year-old boys and 20.3 % of girls the same age smoke. More than one quarter of adolescents who do not smoke themselves are exposed to cigarette smoke several times a week; around one fifth are even exposed to it almost every day. In the case of alcohol, 64.8 % of boys and 63.8 % of girls have drunk it before. Around one third of boys and one quarter of girls indicated that they currently consumed alcohol at least once a week. In the last 12 months before the survey 9.2 % of the boys and 6.2 % of the girls had taken hashish or marijuana. Other drugs such as Ecstasy, amphetamines or speed had been consumed by less than 1 % of the adolescents. The use of psychoactive substances rises markedly as children get older and is thus the most widespread among 16-17-year-olds. Adolescents of low social status smoke more frequently; in the case of alcohol and drug consumption, however, no significant status-specific differences are observed. There is also a raised prevalence of smoking among boys and girls who attend a secondary school and live in the states of the former GDR. The results emphasise the need for an addiction prevention programme which should include intervention to prevent children taking up substance use, as well as withdrawal treatment.
Descriptors
Adolescent, Age Factors, Alcohol Drinking/epidemiology, Child, Cross-Sectional Studies, Female, Germany, Health Surveys, Humans, Male, Marijuana Abuse/epidemiology, Sex Factors, Smoking/epidemiology, Social Environment, Substance-Related Disorders/epidemiology, Tobacco Smoke Pollution/statistics & numerical data
Links
Book Title
Database
Publisher
Data Source
Authors
Lampert,T., Thamm,M.
Original/Translated Title
Tabak-, Alkohol- und Drogenkonsum von Jugendlichen in Deutschland. Ergebnisse des Kinder- und Jugendgesundheitssurveys (KiGGS)
URL
Date of Electronic
PMCID
Editors
Snuffing out cigarette sales and the smoking deaths epidemic 2007 SmokeLess New Zealand Trust, Lyttelton, Christchurch. laugesen@healthnz.co.nz
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
The New Zealand medical journal
Periodical, Abbrev.
N.Z.Med.J.
Pub Date Free Form
15-Jun
Volume
120
Issue
1256
Start Page
U2587
Other Pages
Notes
JID: 0401067; CIN: N Z Med J. 2007;120(1256):U2585. PMID: 17589553; RF: 24; epublish
Place of Publication
New Zealand
ISSN/ISBN
1175-8716; 0028-8446
Accession Number
PMID: 17589555
Language
eng
SubFile
Journal Article; Review; IM
DOI
Output Language
Unknown(0)
PMID
17589555
Abstract
Smokers need new products and policies to escape smoking's risks. And the next generation needs policies that will better protect them from becoming smokers. Low-nitrosamine tobacco snuff (hereafter termed 'snuff') is 20 times less dangerous than cigarette smoking. Its sale as nasal snuff raises the question as to how long cigarettes, including cigars and pipe tobacco, should continue to be sold and allowed to hasten the deaths of 4000 New Zealanders annually. Oral snuff has helped to reduce smoking to unusually low levels in Swedish men, is much less dangerous than smoking, and does not cause lung or mouth cancer. Moreover, smokeless tobacco (which includes snuff) could reduce smoking-caused health inequity for Maori. Snuff can improve population health, and more so if more smokers switch to it. Continued bans on snuff are now regarded by some experts as unsound public policy. Added to the mountain of evidence against cigarettes, sufficient evidence now exists for Government to use snuff to create safer tobacco choices for smokers, end cigarette sales altogether, and thus end the cigarette smoking deaths epidemic--in which 200,000 New Zealanders have died so far. The New Zealand Government can: Fund media campaigns to inform smokers of their new choices, and to urge them to quit smoking. (The 2007 Budget commits an extra $11 million per year for 4 years, an excellent start.) Regulate for warnings on snuff cans stating that snuff is "addictive but much safer than smoking", and regulate imports to only permit reduced-risk low-nitrosamine products. Tax each class of tobacco products proportionate to the respective risks of each. (Tax cigarettes at 20 times the snuff rate, instead of at the same rate.) Legislate, to expand the Smoke-free Environments Act's aims to include ending the sale of cigarettes and ending smoking deaths--i.e: Allow oral snuff to compete with cigarettes for market share (and for the smoker's nicotine receptors). Reduce addiction to smoking, by decreasing the nicotine content of cigarettes by 5% every 6 months. (Below 20% of current levels, most smokers will quit or switch to snuff.), Allot cigarette supply quotas to manufacturers and importers, decreasing by 5% every 6 months, on the grounds that cigarette smoke is irremediably toxic. The summed effects of these changes could end cigarette sales within 10 years, and prevent 90% of cigarette deaths within 22 years thereafter.
Descriptors
Cause of Death, Female, Harm Reduction, Humans, Incidence, Male, New Zealand/epidemiology, Policy Making, Risk Factors, Smoking/mortality/prevention & control, Survival Analysis, Tobacco Industry/legislation & jurisprudence, Tobacco Use Cessation/methods, Tobacco, Smokeless/adverse effects
Links
Book Title
Database
Publisher
Data Source
Authors
Laugesen,M.
Original/Translated Title
URL
Date of Electronic
20070615
PMCID
Editors
Tensile bond strength of Er,Cr:YSGG laser-irradiated human dentin and analysis of dentin-resin interface 2007 Graduate Institute of Clinical Dentistry, College of Medicine, National Taiwan University and National Taiwan University Hospital, Taipei, Taiwan.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Dental materials : official publication of the Academy of Dental Materials
Periodical, Abbrev.
Dent.Mater.
Pub Date Free Form
May
Volume
23
Issue
5
Start Page
570
Other Pages
578
Notes
LR: 20131121; JID: 8508040; 0 (Composite Resins); 0 (Dentin-Bonding Agents); 0 (Methacrylates); 0 (One Coat Bond); 0 (Resin Cements); 0 (palfique estelite); 059QF0KO0R (Water); 2005/07/05 [received]; 2006/03/06 [revised]; 2006/03/09 [accepted]; 2006/07/03
Place of Publication
England
ISSN/ISBN
0109-5641; 0109-5641
Accession Number
PMID: 16820200
Language
eng
SubFile
Journal Article; D
DOI
S0109-5641(06)00104-7 [pii]
Output Language
Unknown(0)
PMID
16820200
Abstract
OBJECTIVES: As the bond strength of composite resin to Er,Cr:YSGG laser-irradiated dentin has not yet been evaluated, the objectives of this study were to investigate the tensile bond strength and to analyze the resin-dentin interface among bur-cut/acid-etched, Er,Cr:YSGG laser-ablated/acid-etched and Er,Cr:YSGG laser-ablated human dentin. METHODS: Crown dentin disks prepared from extracted human third permanent molars were used for the observation of surface morphological changes by scanning electron microscope (SEM). The laser energy parameters were 3.5 W and 20 Hz with water spray (air pressure level, 80%; water pressure level, maximum level). Another group of crown dentin disks were prepared for composite resin restoration and observation of resin-dentin bond interface after demineralization in 6N hydrochloric acid (HCl) for 1 min and deproteinization in 1% sodium hypochlorite solution (NaOCl) for 10 min. The tensile bond strengths of the three groups were measured by a universal testing machine at a crosshead speed of 0.5 mm/min. Fracture types at the dentin-resin interface were analyzed using the digital stereoscopic microscope and fractured surfaces of the debonded specimens were examined by SEM. RESULTS: All three groups showed that the treated surfaces were free of dentin debris and smear layer. The peritubular dentin protruded from the surrounding intertubular dentin after laser irradiation. The dentin-resin interface treated with Er,Cr:YSGG laser irradiation and acid etching demonstrated numerous resin tags converging into a bulge and then diverging again. The length of resin tags was greater than 100 microm. The tensile bond strengths of bur-cut/acid-etched, laser-ablated/acid-etched and laser-ablated human dentin were 5.37+/-1.51, 5.17+/-1.41 and 3.29+/-0.86 MPa, respectively. No statistical significance was found between the bur-cut/acid-etched and laser-ablated/acid-etched groups. The predominant fracture modes of bur-cut/acid-etched, laser-ablated/acid-etched and laser-ablated human dentin were Type 1 (50%), Type 2 (70%) and Type 1 (70%), respectively. SIGNIFICANCE: Er,Cr:YSGG laser irradiation affects adversely adhesion of resin to dentin but acid etching following laser irradiation could increase the tensile bond strength as high as that of bur-cut/acid-etched human dentin.
Descriptors
Acid Etching, Dental, Adolescent, Adult, Composite Resins/chemistry, Dental Bonding, Dental Cavity Preparation/instrumentation/methods, Dentin/ultrastructure, Dentin-Bonding Agents/chemistry, Humans, Lasers, Materials Testing, Methacrylates/chemistry, Microscopy, Electron, Scanning, Resin Cements/chemistry, Smear Layer, Stress, Mechanical, Surface Properties, Tensile Strength, Water/chemistry
Links
Book Title
Database
Publisher
Data Source
Authors
Lee,B. S., Lin,P. Y., Chen,M. H., Hsieh,T. T., Lin,C. P., Lai,J. Y., Lan,W. H.
Original/Translated Title
URL
Date of Electronic
20060703
PMCID
Editors
Immediate impact of smoke-free laws on indoor air quality 2007 College of Public Health, University of Kentucky, 121 Washington Avenue, Lexington, KY 40536-0003, USA. kiyoung@uky.edu
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Southern medical journal
Periodical, Abbrev.
South.Med.J.
Pub Date Free Form
Sep
Volume
100
Issue
9
Start Page
885
Other Pages
889
Notes
JID: 0404522; 0 (Air Pollutants); 0 (Smoke); 0 (Tobacco Smoke Pollution); ppublish
Place of Publication
United States
ISSN/ISBN
0038-4348; 0038-4348
Accession Number
PMID: 17902288
Language
eng
SubFile
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; AIM; IM
DOI
10.1097/SMJ.0b013e31813c6a37 [doi]
Output Language
Unknown(0)
PMID
17902288
Abstract
Smoke-free laws significantly impact indoor air quality. However, the temporal effects of these laws on indoor air pollution have not been determined. This paper assesses the temporal impact of one smoke-free law on indoor air quality. This quasi-experimental study compared the indoor air quality of nine hospitality venues and one bingo hall in Georgetown, Kentucky, before and after implementation of a 100% smoke-free workplace law. We made real-time measurements of particulate matter with 2.5 microm aerodynamic diameter or smaller (PM2.5). Among the nine Georgetown hospitality venues, the average indoor PM2.5 concentration was 84 microg/m3 before the law took effect. The average indoor PM2.5 concentrations in nine compliant venues significantly decreased to 18 microg/m3 one week after the law took effect. Three venues having 82 microg/m3 before the law had significantly lower levels from the first day the law was implemented, and the low level was maintained. Compliance with the law is critical to achieving clean indoor air. Indoor air pollution in the bingo hall was not reduced until the establishment decided to comply with the law. The smoke-free law showed immediate impact on indoor air quality.
Descriptors
Air Pollutants/analysis, Air Pollution, Indoor/analysis/prevention & control, Humans, Kentucky, Recreation, Restaurants, Smoke/analysis, Smoking/legislation & jurisprudence, Smoking Cessation/legislation & jurisprudence, Time Factors, Tobacco Smoke Pollution/analysis/prevention & control
Links
Book Title
Database
Publisher
Data Source
Authors
Lee,K., Hahn,E. J., Riker,C., Head,S., Seithers,P.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Secondhand smoke exposure in a rural high school 2007 Department of Preventive Medicine and Environmental Health, College of Public Health, University of Kentucky, Lexington, KY, USA.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
The Journal of school nursing : the official publication of the National Association of School Nurses
Periodical, Abbrev.
J.Sch.Nurs.
Pub Date Free Form
Aug
Volume
23
Issue
4
Start Page
222
Other Pages
228
Notes
JID: 9206498; 0 (Tobacco Smoke Pollution); ppublish
Place of Publication
United States
ISSN/ISBN
1059-8405; 1059-8405
Accession Number
PMID: 17676970
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; N
DOI
1059-8405-23-4-222 [pii]
Output Language
Unknown(0)
PMID
17676970
Abstract
Although federal law requires all public schools to be smoke free, lack of compliance with the smoke-free policy is commonly reported. The aims of this study were to describe the indoor fine-particle (PM(2.5)) air pollution in a rural high school and surrounding public venues. This cross-sectional, nonexperimental study was conducted in Monroe County, Kentucky (population of 11,756). Fine-particle concentrations were measured in the high school and 5 public venues using spectrometers. Because of illegal student smoking, PM(2.5) concentrations were 19 times higher in the boys' student restroom than the National Ambient Air Quality Standard for outdoor air (670 vs. 35 microg/m(3)). The staff restrooms adjacent to the student restroom where staff did not smoke also showed high PM(2.5) levels. Average indoor air pollution in the public venues was 158 microg/m(3). Strict enforcement of smoke-free school policy and cessation resources are needed to reduce secondhand smoke exposure. Collaborative school-community campaigns involving parents, students, mass media, and community organizations may be effective in reducing the harm caused by tobacco. Implications for school nurses are discussed.
Descriptors
Adolescent, Air Pollution, Indoor/analysis/legislation & jurisprudence/prevention & control, Consumer Participation, Cooperative Behavior, Cross-Sectional Studies, Environmental Monitoring/methods, Female, Health Services Needs and Demand, Humans, Interinstitutional Relations, Kentucky, Male, Mass Media, Nurse's Role, Public Facilities/legislation & jurisprudence/statistics & numerical data, Restaurants/legislation & jurisprudence/statistics & numerical data, Rural Health/statistics & numerical data, School Health Services/legislation & jurisprudence/statistics & numerical data, School Nursing, Schools/organization & administration, Smoking Cessation, Time Factors, Tobacco Smoke Pollution/analysis/legislation & jurisprudence/prevention & control, Toilet Facilities
Links
Book Title
Database
Publisher
Data Source
Authors
Lee,K., Hahn,E. J., Riker,C. A., Hoehne,A., White,A., Greenwell,D., Thompson,D.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Determination of benzene, toluene, ethylbenzene, xylenes in water at sub-ng l-1 levels by solid-phase microextraction coupled to cryo-trap gas chromatography-mass spectrometry 2007 Department of Chemistry, National Chung Hsing University, Taichung 40227, Taiwan, ROC. mrlee@drgon.nchu.edu.tw
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Chemosphere
Periodical, Abbrev.
Chemosphere
Pub Date Free Form
Nov
Volume
69
Issue
9
Start Page
1381
Other Pages
1387
Notes
LR: 20131121; JID: 0320657; 0 (Benzene Derivatives); 0 (Water Pollutants, Chemical); 0 (Xylenes); 3FPU23BG52 (Toluene); J64922108F (Benzene); L5I45M5G0O (ethylbenzene); 2006/12/26 [received]; 2007/05/01 [revised]; 2007/05/03 [accepted]; 2007/06/19 [aheado
Place of Publication
England
ISSN/ISBN
0045-6535; 0045-6535
Accession Number
PMID: 17582460
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
S0045-6535(07)00615-7 [pii]
Output Language
Unknown(0)
PMID
17582460
Abstract
A trace analytical method of benzene, toluene, ethylbenzene and xylenes (BTEX) in water has been developed by using headspace solid-phase microextraction (HS-SPME) coupled to cryo-trap gas chromatography-mass spectrometry (GC-MS). The chromatographic peak shape for BTEX was improved by using cryo-trap equipment. The HS-SPME experimental procedures to extract BTEX from water were optimized with a 75 microm carboxen/polydimethylsiloxane (CAR/PDMS)-coated fiber at a sodium chloride concentration of 267 g l(-1), extraction for 15 min at 25 degrees C and desorption at 290 degrees C for 2 min. Good linearity was verified in a range of 0.0001-50 microg l(-1) for each analyte (r(2)=0.996-0.999). The limits of detection (LODs) of BTEX in water reached at sub-ng l(-1) levels. LODs of benzene, toluene, ethylbenzene, m/p-xylene and o-xylene were 0.04, 0.02, 0.05, 0.01 and 0.02 ng l(-1), respectively. The proposed analytical method was successfully used for the quantification of trace BTEX in ground water. The results indicate that HS-SPME coupled to cryo-trap GC-MS is an effective tool for analysis of BTEX in water samples at the sub-ng l(-1) level.
Descriptors
Benzene/analysis, Benzene Derivatives/analysis, Environmental Monitoring/methods, Freezing, Gas Chromatography-Mass Spectrometry/methods, Reproducibility of Results, Sensitivity and Specificity, Solid Phase Microextraction, Toluene/analysis, Water Pollutants, Chemical/analysis, Xylenes/analysis
Links
Book Title
Database
Publisher
Data Source
Authors
Lee,M. R., Chang,C. M., Dou,J.
Original/Translated Title
URL
Date of Electronic
20070619
PMCID
Editors
Smoking, but not smokers: identity among college students who smoke cigarettes 2007 University of Colorado at Denver and Health Sciences Center, Denver, CO, USA. arnold.levinson@uchsc.edu
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco
Periodical, Abbrev.
Nicotine Tob.Res.
Pub Date Free Form
Aug
Volume
9
Issue
8
Start Page
845
Other Pages
852
Notes
LR: 20151119; JID: 9815751; ppublish
Place of Publication
England
ISSN/ISBN
1462-2203; 1462-2203
Accession Number
PMID: 17654297
Language
eng
SubFile
Journal Article; Research Support, U.S. Gov't, P.H.S.; IM
DOI
780759829 [pii]
Output Language
Unknown(0)
PMID
17654297
Abstract
Cigarette smoking in college is often described as social smoking, but the term lacks definition and implicitly discounts dependence. We report on college students' use of the terms social smoker and smoker. Students who currently smoked cigarettes were asked whether they considered themselves smokers, and whether they smoked because they were social smokers. The survey was conducted during 1999-2004 at eight colleges; analysis was limited to 1,401 students aged 18-24 years. More than half of students (56.3%) denied being smokers ("deniers") despite current smoking behavior. Half of deniers, and fewer than half of admitters, called themselves social smokers. Deniers were highly likely to smoke infrequently, to say they were not addicted to cigarettes, to have mostly nonsmokers as close friends, to prefer dating nonsmokers, and to smoke for reasons other than stress relief. In contrast, social-smoker identity was associated only weakly with any attitude, behavior, or belief. Smoker and social-smoker identities were not significantly correlated with each other. Regardless of identity, more than half of the respondents wanted to quit smoking by graduation. Results suggest that denying being a smoker may be a widespread dissonance among college students who smoke. The possibility should be evaluated using population-level research, because it has potentially undermining implications for smoking cessation campaigns. Campus health centers should avoid using "smoker" self-assessment items on pre-exam questionnaires. Further research is needed to explore the psychosocial mechanisms involved with denier identity, to clarify the implications for public health communications, and to develop appropriate intervention strategies.
Descriptors
Adult, Attitude to Health, Female, Humans, Male, Peer Group, Retrospective Studies, Smoking/epidemiology/psychology, Smoking Cessation/psychology, Social Perception, Students/statistics & numerical data, Surveys and Questionnaires, United States/epidemiology, Universities
Links
Book Title
Database
Publisher
Data Source
Authors
Levinson,A. H., Campo,S., Gascoigne,J., Jolly,O., Zakharyan,A., Tran,Z. V.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Solid-phase extraction with C30 bonded silica for analysis of polycyclic aromatic hydrocarbons in airborne particulate matters by gas chromatography-mass spectrometry 2007 Department of Chemistry, Tsinghua University, Beijing 100084, China.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of chromatography.A
Periodical, Abbrev.
J.Chromatogr.A
Pub Date Free Form
22-Jun
Volume
1154
Issue
2-Jan
Start Page
74
Other Pages
80
Notes
LR: 20090115; JID: 9318488; 0 (Air Pollutants); 0 (Polycyclic Hydrocarbons, Aromatic); 7631-86-9 (Silicon Dioxide); 2007/02/15 [received]; 2007/03/29 [revised]; 2007/03/30 [accepted]; 2007/04/06 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
0021-9673; 0021-9673
Accession Number
PMID: 17459395
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
S0021-9673(07)00657-7 [pii]
Output Language
Unknown(0)
PMID
17459395
Abstract
A solid-phase extraction (SPE) method using triacontyl bonded silica (C30) as sorbent was developed for the determination of 16 US Environmental Protection Agency polycyclic aromatic hydrocarbons (PAHs) in airborne particulate matters quantitatively by gas chromatography-mass spectrometry (GC-MS). Optimization experiments were conducted using spiked standard aqueous solution of PAHs and real airborne particulates samples aiming to obtain highest SPE recoveries and extraction efficiency. Factors were studied in SPE procedures including the concentration of organic modifier, flow rate of sample loading and elution solvents. The ultrasonication time and solvents were also investigated. Recoveries were in the range of 68-107% for standard PAHs aqueous solution and 61-116% for real spiked sample. Limits of detection (LODs) and limits of quantification (LOQs) with standard solution were in the range of 0.0070-0.21 microgL(-1) and 0.022-0.67 microgL(-1), respectively. The optimized method was successfully applied to the determination of 16 PAHs in real airborne particulate matters.
Descriptors
Air Pollutants/analysis, Gas Chromatography-Mass Spectrometry/methods, Polycyclic Hydrocarbons, Aromatic/analysis, Silicon Dioxide, Solid Phase Extraction/methods
Links
Book Title
Database
Publisher
Data Source
Authors
Li,K., Li,H., Liu,L., Hashi,Y., Maeda,T., Lin,J. M.
Original/Translated Title
URL
Date of Electronic
20070406
PMCID
Editors
Detection and characterisation of CTX-M and CMY-2 beta-lactamases among Escherichia coli isolates from farm animals in Guangdong Province of China 2007 College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International journal of antimicrobial agents
Periodical, Abbrev.
Int.J.Antimicrob.Agents
Pub Date Free Form
May
Volume
29
Issue
5
Start Page
576
Other Pages
581
Notes
JID: 9111860; 0 (Anti-Bacterial Agents); EC 3.5.2.6 (beta-Lactamases); 2006/09/20 [received]; 2006/12/22 [revised]; 2006/12/22 [accepted]; 2007/02/20 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
0924-8579; 0924-8579
Accession Number
PMID: 17314033
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
S0924-8579(07)00038-6 [pii]
Output Language
Unknown(0)
PMID
17314033
Abstract
The objective of this study was to characterise the beta-lactamase genes of cephalosporin-resistant Escherichia coli isolated from farm animals in Guangdong Province of China. Of 592 E. coli isolates recovered from farm animals from 2003-2005, 50 (8.4%) showed cephalosporin resistance. Polymerase chain reaction and sequencing analysis showed that 14 isolates (2.4%) from chickens, ducks, pigs and partridges were positive for the bla(CTX-M) gene (10 for bla(CTX-M-14) and 4 for bla(CTX-M-27)). CMY-2 was detected for the first time in mainland China in six E. coli isolates (1.0%) from chickens and goose. Except for one isolate, bla(CTX-M)- and bla(CMY-2)-containing isolates also harboured the bla(TEM-1b) gene. Conjugation experiments demonstrated that the bla(CTX-M) and bla(TEM) genes could be transferred to E. coli DH5alpha. Pulsed-field gel electrophoresis (PFGE) showed that the 14 CTX-M-producing isolates belonged to 12 different types. Two isolates (one from a chicken, the other from a pig) containing CTX-M-14 showed indistinguishable PFGE patterns, indicating clonal dissemination of this strain among animals from different farms. This study describes for the first time the emergence of CTX-M- and CMY-2-producing E. coli among farm animals in China, with the CTX-M-9 group being the predominant extended-spectrum beta-lactamase detected.
Descriptors
Animals, Animals, Domestic/microbiology, Anti-Bacterial Agents/pharmacology, Cephalosporin Resistance, Chickens, China, Cloning, Molecular, Drug Resistance, Bacterial, Ducks, Electrophoresis, Gel, Pulsed-Field, Escherichia coli/drug effects/enzymology, Feces/microbiology, Microbial Sensitivity Tests, Swine, beta-Lactamases/chemistry/genetics/isolation & purification
Links
Book Title
Database
Publisher
Data Source
Authors
Liu,J. H., Wei,S. Y., Ma,J. Y., Zeng,Z. L., Lu,D. H., Yang,G. X., Chen,Z. L.
Original/Translated Title
URL
Date of Electronic
20070220
PMCID
Editors
Oxidized multiwalled carbon nanotubes as a novel solid-phase microextraction fiber for determination of phenols in aqueous samples 2007 College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of chromatography.A
Periodical, Abbrev.
J.Chromatogr.A
Pub Date Free Form
21-Sep
Volume
1165
Issue
2-Jan
Start Page
10
Other Pages
17
Notes
LR: 20131121; JID: 9318488; 0 (Nanotubes, Carbon); 0 (Phenols); 059QF0KO0R (Water); 2007/05/10 [received]; 2007/07/07 [revised]; 2007/07/10 [accepted]; 2007/07/31 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
0021-9673; 0021-9673
Accession Number
PMID: 17707386
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
S0021-9673(07)01251-4 [pii]
Output Language
Unknown(0)
PMID
17707386
Abstract
A simple and environmentally friendly method for determination of seven phenols using solid-phase microextraction (SPME) coupled to high-performance liquid chromatography (HPLC) has been developed. Several materials were used as stationary phase of SPME fibers and an oxidized multiwalled carbon nanotubes material was found to be effective in carrying out simultaneous extraction of phenols in aqueous samples. Compared with the widely used commercially available SPME fibers, this proposed fiber had much lower cost, longer lifetime (over 150 times), shorter analysis time (30 min of extraction and 3 min of desorption time) and comparable or superior extraction efficiency for the investigated analytes. The extraction and desorption conditions were evaluated and the calibration curves of seven phenols were linear (R(2)> or =0.9908) in the range from 10.2 to 1585 ng mL(-1). The limits of detection at a signal-to-noise (S/N) ratio of 3 were 0.25-3.67 ng mL(-1), and the limits of quantification calculated at S/N=10 were 0.83-12.25 ng mL(-1) for these compounds. The possibility of applying the proposed method to environmental water samples analysis was validated.
Descriptors
Chromatography, High Pressure Liquid/methods, Nanotubes, Carbon/ultrastructure, Oxidation-Reduction, Phenols/isolation & purification, Reproducibility of Results, Solid Phase Microextraction/methods, Water/chemistry
Links
Book Title
Database
Publisher
Data Source
Authors
Liu,X., Ji,Y., Zhang,Y., Zhang,H., Liu,M.
Original/Translated Title
URL
Date of Electronic
20070731
PMCID
Editors