Image
WTS Database
The subject of interest is:
waterpipe, water-pipe, nargile, narghile, arguileh, arguile, shisha, sheesha, chichi, hubble bubble, hubbly bubbly, goza, hookah, bong
| Title | Pub Year Sort ascending | Author | SearchLink |
|---|---|---|---|
| Shear bond strength of orthodontic brackets bonded with different self-etching adhesives | 2009 | Professor, Department of Orthodontics, School of Dentistry, Dental Research Center (CIEAO), Autonomous University of the State of Mexico (UAEM), Toluca, Mexico. rogelio_scougall@hotmail.com | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, its Constituent Societies, and the American Board of Orthodontics
Periodical, Abbrev.
Am.J.Orthod.Dentofacial Orthop.
Pub Date Free Form
Sep
Volume
136
Issue
3
Start Page
425
Other Pages
430
Notes
LR: 20131121; JID: 8610224; 0 (Acrylic Resins); 0 (AdheSE cement); 0 (Beauty Ortho Bond); 0 (Clearfil Bonding Agent); 0 (Dental Alloys); 0 (Kurasper F); 0 (Phosphoric Acids); 0 (Resin Cements); 0 (Transbond Plus Self-Etching Primer); 0 (Transbond XT); 059
Place of Publication
United States
ISSN/ISBN
1097-6752; 0889-5406
Accession Number
PMID: 19732677
Language
eng
SubFile
Comparative Study; Journal Article; Randomized Controlled Trial; D; IM
DOI
10.1016/j.ajodo.2007.08.024 [doi]
Output Language
Unknown(0)
PMID
19732677
Abstract
INTRODUCTION: The purpose of this study was to compare the shear bond strength (SBS) of orthodontic brackets bonded with 4 self-etching adhesives. METHODS: A total of 175 extracted premolars were randomly divided into 5 groups (n = 35). Group I was the control, in which the enamel was etched with 37% phosphoric acid, and stainless steel brackets were bonded with Transbond XT (3M Unitek, Monrovia, Calif). In the remaining 4 groups, the enamel was conditioned with the following self-etching primers and adhesives: group II, Transbond Plus and Transbond XT (3M Unitek); group III, Clearfil Mega Bond FA and Kurasper F (Kuraray Medical, Tokyo, Japan); group IV, Primers A and B, and BeautyOrtho Bond (Shofu, Kyoto, Japan); and group V, AdheSE and Heliosit Orthodontic (Ivoclar Vivadent AG, Liechtenstein). The teeth were stored in distilled water at 37 degrees C for 24 hours and debonded with a universal testing machine. The adhesive remnant index (ARI) including enamel fracture score was also evaluated. Additionally, the conditioned enamel surfaces were observed under a scanning electron microscope. RESULTS: The SBS values of groups I (19.0 +/- 6.7 MPa) and II (16.6 +/- 7.3 MPa) were significantly higher than those of groups III (11.0 +/- 3.9 MPa), IV (10.1 +/- 3.7 MPa), and V (11.8 +/- 3.5 MPa). Fluoride-releasing adhesives (Kurasper F and BeautyOrtho Bond) showed clinically acceptable SBS values. Significant differences were found in the ARI and enamel fracture scores between groups I and II. CONCLUSIONS: The 4 self-etching adhesives yielded SBS values higher than the bond strength (5.9 to 7.8 MPa) suggested for routine clinical treatment, indicating that orthodontic brackets can be successfully bonded with any of these self-etching adhesives.
Descriptors
Acid Etching, Dental, Acrylic Resins/chemistry, Adhesiveness, Dental Alloys/chemistry, Dental Bonding, Dental Enamel/injuries/ultrastructure, Dental Stress Analysis/instrumentation, Humans, Materials Testing, Microscopy, Electron, Scanning, Orthodontic Brackets, Phosphoric Acids/chemistry, Resin Cements/chemistry, Shear Strength, Stainless Steel/chemistry, Stress, Mechanical, Surface Properties, Temperature, Time Factors, Water/chemistry
Links
Book Title
Database
Publisher
Data Source
Authors
Scougall Vilchis,R. J., Yamamoto,S., Kitai,N., Yamamoto,K.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
|
|||
| Identification of lactic acid bacteria isolated from Tarhana, a traditional Turkish fermented food | 2009 | Department of Food Engineering, Food Microbiology Section, Faculty of Engineering, University of Ege, Turkey. ilkin.sengun@ege.edu.tr | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International journal of food microbiology
Periodical, Abbrev.
Int.J.Food Microbiol.
Pub Date Free Form
31-Oct
Volume
135
Issue
2
Start Page
105
Other Pages
111
Notes
JID: 8412849; 0 (Carbohydrates); 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); 2008/11/04 [received]; 2009/07/22 [revised]; 2009/07/23 [accepted]; 2009/08/04 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
1879-3460; 0168-1605
Accession Number
PMID: 19703719
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.ijfoodmicro.2009.07.033 [doi]
Output Language
Unknown(0)
PMID
19703719
Abstract
Tarhana is a traditional fermented product produced from a mixture of spontaneously fermented yogurt and wheat flour in Turkey. The aims of the present study were to enumerate and identify for the first time by molecular biology-based methods predominant lactic acid bacteria (LAB) isolated during processing of Tarhana. Samples were collected from eight different regions of Turkey. In order to explore the relationship between raw material and the microbiology of Tarhana, yogurt and wheat flour were also analyzed. A total of 226 Gram-positive and catalase-negative isolates were obtained from MRS, M17 and SBM (Slanetz and Bartley Medium). The isolates were grouped and identified using a combination of pheno- and genotypic methods including rep-PCR fingerprinting [(GTG)(5) primer], multiplex PCR, 16S rRNA gene sequencing and carbohydrate assimilation profiling. Pediococcus acidilactici were found to constitute 27% of the isolates, 19% were identified as Streptococcus thermophilus, 19% as Lactobacillus fermentum, 12% as Enterococcus faecium, 7% as Pediococcus pentosaceus, 5% as Leuconostoc pseudomesenteroides, 4% as Weissella cibaria, 2% as Lactobacillus plantarum, 2% as Lactobacillus delbrueckii spp. bulgaricus, 2% as Leuconostoc citreum, 1% as Lactobacillus paraplantarum and 0.5% as Lactobacillus casei. The different production sites investigated all had individual LAB profiles, but with P. acidilactici and S. thermophilus being isolated from the majority of samples. The main source of P. acidilactici and S. thermophilus was found to be the yogurt.
Descriptors
Bacterial Typing Techniques, Biodiversity, Carbohydrates, Colony Count, Microbial, DNA, Bacterial, DNA, Ribosomal, Fermentation, Flour/microbiology, Food Handling, Food Microbiology, Genotype, Humans, Lactobacillaceae/classification/genetics/isolation & purification, Phenotype, Polymerase Chain Reaction, RNA, Ribosomal, 16S, Sequence Analysis, RNA, Triticum/microbiology, Turkey, Yogurt/microbiology
Links
Book Title
Database
Publisher
Data Source
Authors
Sengun,I. Y., Nielsen,D. S., Karapinar,M., Jakobsen,M.
Original/Translated Title
URL
Date of Electronic
20090804
PMCID
Editors
|
|||
| N'-[(E)-(4-Bromo-2-thien-yl)methyl-idene]benzohydrazide 0.06-hydrate | 2009 | Read more... | |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Acta crystallographica.Section E, Structure reports online
Periodical, Abbrev.
Acta Crystallogr.Sect.E.Struct.Rep.Online
Pub Date Free Form
19-Sep
Volume
65
Issue
Pt 10
Start Page
o2501
Other Pages
Notes
LR: 20111209; JID: 101089178; OID: NLM: PMC2970472; 2009/09/12 [received]; 2009/09/15 [accepted]; 2009/09/19 [epublish]; epublish
Place of Publication
England
ISSN/ISBN
1600-5368; 1600-5368
Accession Number
PMID: 21577950
Language
eng
SubFile
Journal Article
DOI
10.1107/S1600536809037350 [doi]
Output Language
Unknown(0)
PMID
21577950
Abstract
The title compound, C(12)H(9)BrN(2)OS.0.06H(2)O, is a hydrated Schiff base derived from benzoic hydrazide and 4-bromo-thio-phene-2-carboxaldehide. The two Schiff base mol-ecules in the asymmetric unit differ crystallographically: in one mol-ecule the dihedral angle between the benzene ring and thio-phene ring is 49.88 (11) degrees , whereas the other mol-ecule the rings are almost coplanar with an r.m.s. deviation for the non-H atoms of 0.025 A. In the crystal, mol-ecules form polymeric sheets linked by N-Hcdots, three dots, centeredO and C-Hcdots, three dots, centeredO hydrogen bonds. The water mol-ecule of crystallization is partially occupied and its H atoms could not be located.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Shafiq,Z., Yaqub,M., Tahir,M. N., Hussain,A., Iqbal,M. S.
Original/Translated Title
URL
Date of Electronic
20090919
PMCID
PMC2970472
Editors
|
|||
| Tobacco smoking pattern: cigarette vs. hookah | 2009 | Read more... | |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Tanaffos
Periodical, Abbrev.
Pub Date Free Form
Volume
8
Issue
1
Start Page
62
Other Pages
67
Notes
ID: 102869
Place of Publication
ISSN/ISBN
Accession Number
Language
english
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
Smoking causes 5.2 million deaths annually in the world of which 70% occur in developing countries. Hookah smoking is increasing around the world especially in the Eastern Mediterranean Region including Iran. This study was carried out to evaluate the pattern of tobacco smoking in both forms of cigarette and hookah smoking. A cross- sectional study was conducted among a random population in the main squares of Tehran in 2006. The sample size consisted of 2053 people in the age range of 10 to 80 years. Non-Probability Sampling method was used. Questionnaires designed and adapted according to WHO and IUATLD questionnaires given to these people. Forty-six percent of the sample had experienced hookah smoking. The prevalence of occasional hookah smoking in the previous year was 45%, while 10% of the participants used hookah at least once a week, 17.9% at least once a month and 17.1% at least once a year;47.2% of participants had experienced cigarette smoking. Prevalence of daily cigarette smoking was 22.7%; 22.7% of current smokers and 25.01% of non-smokers consumed hookah at least once a week. Prevalence of hookah smoking is very similar among cigarette smokers and non-smokers. In this study the prevalence of cigarette smokers was more than national data and the rate of cigarette and hookah smoking among women was higher than that of other studies in this realm. These issues need to be further investigated and more serious studies are required in this regard
Descriptors
Humans, Male, Female, Abdomen, Acute - 13 YEARS, 13 - 19 YEARS, ABO Blood-Group System - 45 YEARS, Absorption - 64 YEARS, OVER 64 YEARS, Comparative Study, Smoking/epidemiology, Tobacco, Tobacco Smoke Pollution, Cross-Sectional Studies, Questionnaires
Links
http://www.nritld.ac.ir/portals/tanaffosjournal/2009/29/dr%20masjedi.pdf
Book Title
Database
GHL; http://www.globalhealthlibrary.net/
Publisher
Data Source
Authors
Sharifi,Hooman, Heydari,Gholam Reza, Emami,Habib, Kashani,Babak Sharif, Tafti,Saeed Fallah, Masjedi,Mohammad Reza
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
|
|||
| Association between risky drinking behaviors and risk groups of substance abuse: a study in Japanese college freshmen | 2009 | Department of Drug Dependence Research, National Institute of Mental Health, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashicho, Kodaira, Tokyo 187-8533, Japan. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Nihon Arukoru Yakubutsu Igakkai zasshi = Japanese journal of alcohol studies & drug dependence
Periodical, Abbrev.
Nihon Arukoru Yakubutsu Igakkai Zasshi
Pub Date Free Form
Dec
Volume
44
Issue
6
Start Page
649
Other Pages
658
Notes
LR: 20151119; JID: 9612304; ppublish
Place of Publication
Japan
ISSN/ISBN
1341-8963; 1341-8963
Accession Number
PMID: 20077759
Language
jpn
SubFile
English Abstract; Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
Output Language
Unknown(0)
PMID
20077759
Abstract
This study examines the prevalence of risky drinking and the association between risky drinking behaviors and risk groups of substance abuse among college freshmen. A total of 376 college freshmen (126 boys and 248 girls) in a Japanese university participated in the study. The subjects were asked to complete self-administered, anonymous questionnaires during their class. The number of participants who had used drugs was small. The following 2 items for substance abuse were included in the questionnaires: (1) those who had drug using peers who used drugs and (2) those who had been persuaded to use drugs by their peers. On the basis of the responses, the participants were classified into 3 groups: (1) high-risk group (HRG), which accounted for 1.4% of the subjects and comprised those who answered "yes" to both the above-mentioned two items; (2) risk group (RG), which accounted for 7.4% and comprised participants who answered "yes" to one of the two items; and (3) control group (CG), which accounted for 91.2% and comprised those who did not answer "yes" to either of the two items. Bivariate analyses were performed to evaluate the association between risky drinking behaviors and risk groups of substance abuse. The results of our study indicated that 87.0% of the participants reported lifetime alcohol use, and 69.4% reported the consumption of alcohol during the past 30 days. Of the former group, 21.4% had engaged in binge drinking, 8.6% had experienced alcohol-related harassment, 9.5% had experienced alcohol-induced blackouts, and 82.0% had experienced drinking alcohol with peers without adults. There were significant associations between risk groups of substance abuse and risky drinking such as binge drinking (p = 0.001), alcohol-induced blackouts (p = 0.020), alcohol-related harassment (p = 0.012), alcohol consumption during the past 30 days (p = 0.047). However, lifetime alcohol use (p = 0.264) and experience of drinking alcohol with peers without adults (p = 0.103) did not differ significantly. These findings indicated that risky drinking behavior such as binge drinking or alcohol-induced blackouts are associated with substance abuse among college freshmen. Substance abuse prevention programs for college students should address the health effects of risky drinking behaviors and train students how to avoid submission to peer pressure.
Descriptors
Adolescent, Adult, Alcohol Drinking/epidemiology/psychology, Female, Humans, Japan/epidemiology, Male, Prevalence, Risk, Students/psychology, Substance-Related Disorders/epidemiology/psychology, Surveys and Questionnaires, Universities, Young Adult
Links
Book Title
Database
Publisher
Data Source
Authors
Shimane,T., Wada,K., Mishima,K., Fujiwara,M.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
|
|||
| Nursing interventions in tobacco-dependent patients with cardiovascular diseases | 2009 | Read more... | |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Annu Rev Nurs Res
Periodical, Abbrev.
Annu.Rev.Nurs.Res.
Pub Date Free Form
Volume
27
Issue
Start Page
221
Other Pages
42
Notes
ID: 20192106
Place of Publication
ISSN/ISBN
Accession Number
Language
en
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
Smoking is a well-established risk factor for cardiovascular disease (CVD). This chapter provides an overview of a program of nursing research relevant to tobacco use in patients with CVD. The Women's Initiative for Nonsmoking (WINS) provides a rich demonstration of a key randomized clinical trial (RCT) on the efficacy of smoking cessation in women. The National Institutes of Health priority for data mining of existing RCTs is demonstrated in the numerous presentations of findings from secondary papers from WINS that answer additional research questions relevant to smoking cessation, including the influence of depression on smoking, myths about and underuse of nicotine replacement therapy. The methodological and logistical challenges inherent in tobacco intervention studies are presented, including a discussion of research needed in the measurement of withdrawal symptoms. Additionally, the role and contributions of nurses serving on the federal guideline development process are highlighted. International research activities of the coauthors from Jordan and Korea are also presented, including a discussion of the need for research in waterpipe use.
Descriptors
Cardiovascular Diseases/nursing, Smoking Cessation/methods, Tobacco Use Disorder/nursing, Cardiovascular Diseases/epidemiology, Cardiovascular Diseases/rehabilitation, Clinical Trials as Topic, Female, Humans, Jordan/epidemiology, Korea/epidemiology, Male, Nursing Research, Practice Guidelines as Topic, Tobacco Use Disorder/epidemiology, Tobacco Use Disorder/rehabilitation, United States/epidemiology, Women&apos, s Health
Links
http://pesquisa.bvsalud.org/ghl/resource/en/mdl-20192106
Book Title
Database
MEDLINE; http://www.globalhealthlibrary.net/
Publisher
Data Source
Authors
Shishani,Kawkab, Sohn,Min, Okada,Ayako, Froelicher,Erika Sivarajan
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
|
|||
| Medical students' attitudes towards tobacco smoking at the first and sixth year of their studies 2002-2008 | 2009 | Klinika Alergologii Gdanskiego Uniwersytetu Medycznego, Gdansk. asieminska@amg.gda.pl | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Pneumonologia i alergologia polska
Periodical, Abbrev.
Pneumonol.Alergol.Pol.
Pub Date Free Form
Volume
77
Issue
4
Start Page
371
Other Pages
379
Notes
LR: 20151119; JID: 9302892; CIN: Pneumonol Alergol Pol. 2010;78(2):176-81; author reply 182-5. PMID: 20306430; ppublish
Place of Publication
Poland
ISSN/ISBN
0867-7077; 0867-7077
Accession Number
PMID: 19722142
Language
pol
SubFile
English Abstract; Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
19722142
Abstract
INTRODUCTION: Smoking habit among medical students indicates that studying of medicine does not sufficiently protect from tobacco use. The aim of the study was an analysis of medical students' attitudes towards smoking during at the first and sixth year of their studies. MATERIAL AND METHODS: A questionnaire on tobacco smoking was distributed among medical students of the study year 2002-2008 at the first and sixth year of their studies. The questionnaire used on sixth year included additional questions which enabled to assess changes in students' attitudes towards smoking during studies, to know respondents opinion on teaching of diagnostics and treatment of tobacco dependence (TD), and to know how they evaluated their knowledge on this issue. The numbers of students who participated at two points of the study were 287 and 175 respectively. RESULTS: Students of VI year significantly less frequently smoked cigarettes regularly than at the beginning of the medical education (13% v. 21%; p=0.022). However, 20% of smokers started smoking during studies. The rate of smokers declaring not to be embarrassed by their smoking habit was significantly lower among sixth-year students in comparison to population of first-year students (31% v. 70%; p=0.0006), as well as the rates of those who declared the will to quit smoking (91% v. 61%), and those who wished to undergo treatment for TD (54% v. 22%) were significantly higher (p=0.013 and p=0.001, respectively). Over a half (57%) of sixth-year students claimed that they had no knowledge on the diagnostics and treatment of TD or their knowledge on this issue was poor/very poor, and in opinion of 43% of students medical curriculum was not a good source of knowledge on TD. CONCLUSIONS: Medical studies induce positive students' attitudes towards smoking. However, a proportion of individuals start smoking during studies, what may suggest dominance of genetic influences on smoking initiation in this period of life. In sixth-year students' opinion medical studies are not a sufficient source of knowledge on TD.
Descriptors
Adult, Attitude to Health, Chi-Square Distribution, Female, Health Behavior, Humans, Male, Poland/epidemiology, Prevalence, Sex Distribution, Smoking/epidemiology/prevention & control/psychology, Socioeconomic Factors, Students, Medical/psychology/statistics & numerical data, Surveys and Questionnaires, Time Factors, Tobacco Use Disorder/epidemiology/prevention & control/psychology, Universities, Young Adult
Links
Book Title
Database
Publisher
Data Source
Authors
Sieminska,A., Jassem-Bobowicz,J. M., Uherek,M., Wilanowski,T., Nowak,R., Jassem,E.
Original/Translated Title
Postawy wobec palenia tytoniu wsrod studentow I i VI roku medycyny z rocznika studiow 2002-2008
URL
Date of Electronic
PMCID
Editors
|
|||
| Taxonomic structure and monitoring of the dominant population of lactic acid bacteria during wheat flour sourdough type I propagation using Lactobacillus sanfranciscensis starters | 2009 | Dipartimento di Protezione delle Piante e Microbiologia Applicata, Universita degli Studi di Bari, Bari, Italy. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Applied and Environmental Microbiology
Periodical, Abbrev.
Appl.Environ.Microbiol.
Pub Date Free Form
Feb
Volume
75
Issue
4
Start Page
1099
Other Pages
1109
Notes
LR: 20140901; JID: 7605801; 0 (Amino Acids); 0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); EC 2.7.7.- (Rec A Recombinases); EC 2.7.7.6 (DNA-Directed RNA Polymerases); EC 2.7.7.6 (RNA polymerase beta subunit); OID
Place of Publication
United States
ISSN/ISBN
1098-5336; 0099-2240
Accession Number
PMID: 19088320
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1128/AEM.01524-08 [doi]
Output Language
Unknown(0)
PMID
19088320
Abstract
The structure and stability of the dominant lactic acid bacterium population were assessed during wheat flour sourdough type I propagation by using singly nine strains of Lactobacillus sanfranciscensis. Under back-slopping propagation with wheat flour type 0 F114, cell numbers of presumptive lactic acid bacteria varied slightly between and within starters. As determined by randomly amplified polymorphic DNA-PCR and restriction endonuclease analysis-pulsed-field gel electrophoresis analyses, only three (LS8, LS14, and LS44) starters dominated throughout 10 days of propagation. The others progressively decreased to less than 3 log CFU g(-1). Partial sequence analysis of the 16S rRNA and recA genes and PCR-denaturating gradient gel electrophoresis analysis using the rpoB gene allowed identification of Weissella confusa, Lactobacillus sanfranciscensis, Lactobacillus plantarum, Lactobacillus rossiae, Lactobacillus brevis, Lactococcus lactis subsp. lactis, Pediococcus pentosaceus, and Lactobacillus spp. as the dominant species of the raw wheat flour. At the end of propagation, one autochthonous strain of L. sanfranciscensis was found in all the sourdoughs. Except for L. brevis, strains of the above species were variously found in the mature sourdoughs. Persistent starters were found in association with other biotypes of L. sanfranciscensis and with W. confusa or L. plantarum. Sourdoughs were characterized for acidification, quotient of fermentation, free amino acids, and community-level catabolic profiles by USING Biolog 96-well Eco microplates. In particular, catabolic profiles of sourdoughs containing persistent starters behaved similarly and were clearly differentiated from the others. The three persistent starters were further used for the production of sourdoughs and propagated by using another wheat flour whose lactic acid bacterium population in part differed from the previous one. Also, in this case all three starter strains persisted during propagation.
Descriptors
Amino Acids/analysis, Bacterial Proteins/genetics, Colony Count, Microbial, DNA Fingerprinting, DNA, Bacterial/genetics, DNA-Directed RNA Polymerases/genetics, Electrophoresis/methods, Electrophoresis, Gel, Pulsed-Field, Flour/microbiology, Food Analysis, Food Microbiology, Gram-Positive Bacteria/classification/genetics/growth & development/isolation & purification, Hydrogen-Ion Concentration, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Random Amplified Polymorphic DNA Technique, Rec A Recombinases/genetics, Triticum/microbiology
Links
Book Title
Database
Publisher
Data Source
Authors
Siragusa,S., Di Cagno,R., Ercolini,D., Minervini,F., Gobbetti,M., De Angelis,M.
Original/Translated Title
URL
Date of Electronic
20081216
PMCID
PMC2643576
Editors
|
|||
| Development of a PCR assay for the strain-specific identification of probiotic strain Lactobacillus paracasei IMPC2.1 | 2009 | Institute of Sciences of Food Production, National Research Council, Bari, Italy. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International journal of food microbiology
Periodical, Abbrev.
Int.J.Food Microbiol.
Pub Date Free Form
30-Nov
Volume
136
Issue
1
Start Page
59
Other Pages
65
Notes
GENBANK/EU187503; JID: 8412849; 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 2009/05/18 [received]; 2009/09/15 [revised]; 2009/09/17 [accepted]; 2009/09/26 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
1879-3460; 0168-1605
Accession Number
PMID: 19833402
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.ijfoodmicro.2009.09.017 [doi]
Output Language
Unknown(0)
PMID
19833402
Abstract
Recent investigations clearly indicate that the probiotic bacterium Lactobacillus paracasei IMPC2.1 can be incorporated into vegetables to obtain innovative probiotic foods whose marketing has been authorized by the Italian Ministry of Health. In this study, strain IMPC2.1 was characterized at a molecular level in order to define its taxonomic position and to develop a PCR test for strain-specific identification. Molecular methods, such as 16S rRNA gene sequencing and multiplex PCR, have provided evidence that strain IMPC2.1 indeed belongs to the L. paracasei species. In addition, a cluster analysis of fluorescent amplified fragment length polymorphism (f-AFLP) data strongly indicated that strain IMPC2.1 and nine other L. paracasei strains (including strain ATCC 334) belong to the same species and are definitely differentiated from the type strain L. casei ATCC 393. The f-AFLP technique was also used to identify a strain-specific DNA fragment of L. paracasei IMPC2.1 - encoding an amino acid sequence similar to a glycosyltransferase of probiotic strain Lactobacillus rhamnosus HN001 - which enabled us to develop a rapid PCR test for strain-specific identification. The strain-specificity of the PCR test was assessed by comparison with a total of 73 bacterial strains mainly isolated from vegetable products that did not produce any amplified fragment. These strains belonged to the L. paracasei species, to 6 additional species of Lactobacillus and to Weissella cibaria, W. confusa, Lactococcus lactis, Leuconostoc mesenteroides and Pediococcus pentosaceus. A method similar to the one used in this study can be adopted to develop easy, rapid detection techniques for monitoring other bacteria in complex microbiota.
Descriptors
Amplified Fragment Length Polymorphism Analysis, Bacterial Typing Techniques/methods, Biodiversity, DNA, Bacterial/genetics, Food Microbiology, Lactobacillus/classification/genetics/growth & development/isolation & purification, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction/methods, Probiotics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity
Links
Book Title
Database
Publisher
Data Source
Authors
Sisto,A., De Bellis,P., Visconti,A., Morelli,L., Lavermicocca,P.
Original/Translated Title
URL
Date of Electronic
20090926
PMCID
Editors
|
|||
| Tobacco use among students aged 13-15 years--Baghdad, Iraq, 2008 | 2009 | Read more... | |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
MMWR Morb Mortal Wkly Rep
Periodical, Abbrev.
MMWR Morb.Mortal.Wkly.Rep.
Pub Date Free Form
Volume
58
Issue
12
Start Page
305
Other Pages
8
Notes
ID: 19343010
Place of Publication
ISSN/ISBN
Accession Number
Language
en
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
In 2008, Iraq's parliament ratified the World Health Organization Framework Convention on Tobacco Control (WHO FCTC), which obligates participants to establish tobacco use monitoring, surveillance, and evaluation systems. Lack of data on adolescent tobacco use in Iraq led the Ministry of Health (MOH) to conduct the Global Youth Tobacco Survey (GYTS) in Baghdad in 2008. GYTS is a school-based survey of students aged 13--15 years that is self-administered in classes in selected schools. As in most Middle East countries, tobacco use in Iraq takes the form of cigarettes and shisha. Based on GYTS results, 7.4% of students aged 13--15 years reported having ever smoked cigarettes, 12.9% had ever smoked shisha, 3.2% currently smoked cigarettes, and 6.3% currently smoked shisha. Among never smokers aged 13--15 years, 13.0% reported they were likely to initiate cigarette smoking in the next year. Future declines in adolescent tobacco use in Iraq (and Baghdad) could be enhanced by expanding existing tobacco control programs to include prevention and cessation of the use of cigarettes and shisha, implementing measures that discourage adolescents who have never smoked from initiating tobacco use, expanding legislation to ban exposure to secondhand smoke in all indoor workplaces, and enacting legislation banning pro-tobacco advertising and sponsorship.
Descriptors
Smoking/epidemiology, Students, Adolescent, Advertising as Topic, Data Collection, Female, Humans, Iraq/epidemiology, Male, Public Policy, Tobacco Smoke Pollution/statistics & numerical data
Links
http://pesquisa.bvsalud.org/ghl/resource/en/mdl-19343010
Book Title
Database
MEDLINE; http://www.globalhealthlibrary.net/
Publisher
Data Source
Authors
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
|
|||