WTS database

Title	Pub Year	Author	SearchLink Sort descending
Crystal structure of 4-chloro-N-{[1-(4-chloro-benzo-yl)piperidin-4-yl]meth-yl}benzamide monohydrate	2014	PG and Research Department of Physics, Queen Mary's College, Chennai-4, Tamilnadu, India.; Department of Chemistry, Madras Christian College, Chennai-59, India.; PG and Research Department of Physics, Queen Mary's College, Chennai-4, Tamilnadu, India.; PG	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Acta crystallographica.Section E, Structure reports online Periodical, Abbrev. Acta Crystallogr.Sect.E.Struct.Rep.Online Pub Date Free Form 3-Sep Volume 70 Issue Pt 10 Start Page o1080 Other Pages Notes LR: 20141215; JID: 101089178; OID: NLM: PMC4257193; OTO: NOTNLM; 2014/10/01 [ecollection]; 2014/07/24 [received]; 2014/08/14 [accepted]; 2014/09/03 [epublish]; epublish Place of Publication United States ISSN/ISBN 1600-5368; 1600-5368 Accession Number PMID: 25484682 Language eng SubFile Journal Article DOI 10.1107/S1600536814018522 [doi] Output Language Unknown(0) PMID 25484682 Abstract In the title compound, C20H20Cl2N2O2.H2O, the piperidine ring adopts a chair conformation with the two substituent benzene rings inclined to one another [dihedral angle 84.63 (9) degrees ]. In the crystal, the components are linked by Ow-Hcdots, three dots, centeredO, N-Hcdots, three dots, centeredOw (w = water) and C-Hcdots, three dots, centeredO hydrogen bonds, generating a sheet structure lying parallel to (101). Descriptors Links Book Title Database Publisher Data Source Authors Prathebha,K., Reuben Jonathan,D., Sathya,S., Jovita,J., Usha,G. Original/Translated Title URL Date of Electronic 20140903 PMCID PMC4257193 Editors
Crystal structure of bis-[(5-amino-1H-1,2,4-triazol-3-yl-kappaN (4))acetato-kappaO]di-aqua-nickel(II) dihydrate	2014	South-Russia State Technical University Prosveschenya, 132, Novocherkassk, Rostov Region, 346428, Russian Federation.; South-Russia State Technical University Prosveschenya, 132, Novocherkassk, Rostov Region, 346428, Russian Federation.; South-Russia Stat	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Acta crystallographica.Section E, Structure reports online Periodical, Abbrev. Acta Crystallogr.Sect.E.Struct.Rep.Online Pub Date Free Form 4-Oct Volume 70 Issue Pt 11 Start Page 286 Other Pages 289 Notes LR: 20141215; JID: 101089178; OID: NLM: PMC4257300; OTO: NOTNLM; 2014/11/01 [ecollection]; 2014/09/19 [received]; 2014/09/26 [accepted]; 2014/10/04 [epublish]; epublish Place of Publication United States ISSN/ISBN 1600-5368; 1600-5368 Accession Number PMID: 25484724 Language eng SubFile Journal Article DOI 10.1107/S1600536814021436 [doi] Output Language Unknown(0) PMID 25484724 Abstract The title compound, [Ni(C4H5N4O2)2(H2O)2].2H2O, represents the first transition metal complex of the novel chelating triazole ligand, 2-(5-amino-1H-1,2,4-triazol-3-yl)acetic acid (ATAA), to be structurally characterized. In the mol-ecule of the title complex, the nickel(II) cation is located on an inversion centre and is coordinated by two water mol-ecules in axial positions and two O and two N atoms from two trans-oriented chelating anions of the deprotonated ATAA ligand, forming a slightly distorted octa-hedron. The trans angles of the octa-hedron are all 180 degrees due to the inversion symmetry of the mol-ecule. The cis-angles are in the range 87.25 (8)-92.75 (8) degrees . The six-membered chelate ring adopts a slightly twisted boat conformation with puckering parameters Q = 0.542 (2) A, Theta = 88.5 (2) and varphi = 15.4 (3) degrees . The mol-ecular conformation is stabilized by intra-molecular N-Hcdots, three dots, centeredO hydrogen bonds between the amino group and the chelating carboxyl-ate O atom of two trans-oriented ligands. In the crystal, the complex mol-ecules and lattice water mol-ecules are linked into a three-dimensional framework by an extensive network of N-Hcdots, three dots, centeredO, O-Hcdots, three dots, centeredO and O-Hcdots, three dots, centeredN hydrogen bonds. Descriptors Links Book Title Database Publisher Data Source Authors Chernyshev,V.M., Chernysheva,A.V., Abagyan,R.S., Rybakov,V.B. Original/Translated Title URL Date of Electronic 20141004 PMCID PMC4257300 Editors
Associations between initial water pipe tobacco smoking and snus use and subsequent cigarette smoking: results from a longitudinal study of US adolescents and young adults	2015	Norris Cotton Cancer Center, Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire2Dartmouth Institute for Health Policy and Clinical Practice, Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire.; Norris Cotton Cancer Center, Geisel S	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full JAMA pediatrics Periodical, Abbrev. JAMA Pediatr. Pub Date Free Form Feb Volume 169 Issue 2 Start Page 129 Other Pages 136 Notes LR: 20151028; GR: KL2 TR001088/TR/NCATS NIH HHS/United States; GR: KL2TR001088/TR/NCATS NIH HHS/United States; GR: P30 CA023108/CA/NCI NIH HHS/United States; GR: R01 CA077026/CA/NCI NIH HHS/United States; GR: R01 CA140150/CA/NCI NIH HHS/United States; GR: Place of Publication United States ISSN/ISBN 2168-6211; 2168-6203 Accession Number PMID: 25485959 Language eng SubFile Journal Article; Research Support, N.I.H., Extramural; AIM; IM DOI 10.1001/jamapediatrics.2014.2697 [doi] Output Language Unknown(0) PMID 25485959 Abstract IMPORTANCE: Many adolescents and young adults use alternative tobacco products, such as water pipes and snus, instead of cigarettes. OBJECTIVE: To assess whether prior water pipe tobacco smoking and snus use among never smokers are risk factors for subsequent cigarette smoking. DESIGN, SETTING, AND PARTICIPANTS: We conducted a 2-wave national longitudinal study in the United States among 2541 individuals aged 15 to 23 years old. At baseline (October 25, 2010, through June 11, 2011), we ascertained whether respondents had smoked cigarettes, smoked water pipe tobacco, or used snus. At the 2-year follow-up (October 27, 2012, through March 31, 2013), we determined whether baseline non-cigarette smokers had subsequently tried cigarette smoking, were current (past 30 days) cigarette smokers, or were high-intensity cigarette smokers. We fit multivariable logistic regression models among baseline non-cigarette smokers to assess whether baseline water pipe tobacco smoking and baseline snus use were associated with subsequent cigarette smoking initiation and current cigarette smoking, accounting for established sociodemographic and behavioral risk factors. We fit similarly specified multivariable ordinal logistic regression models to assess whether baseline water pipe tobacco smoking and baseline snus use were associated with high-intensity cigarette smoking at follow-up. EXPOSURES: Water pipe tobacco smoking and the use of snus at baseline. MAIN OUTCOMES AND MEASURES: Among baseline non-cigarette smokers, cigarette smoking initiation, current (past 30 days) cigarette smoking at follow-up, and the intensity of cigarette smoking at follow-up. RESULTS: Among 1596 respondents, 1048 had never smoked cigarettes at baseline, of whom 71 had smoked water pipe tobacco and 20 had used snus at baseline. At follow-up, accounting for behavioral and sociodemographic risk factors, baseline water pipe tobacco smoking and snus use were independently associated with cigarette smoking initiation (adjusted odds ratios: 2.56; 95% CI, 1.46-4.47 and 3.73; 95% CI, 1.43-9.76, respectively), current cigarette smoking (adjusted odds ratios: 2.48; 95% CI, 1.01-6.06 and 6.19; 95% CI, 1.86-20.56, respectively), and higher intensity of cigarette smoking (adjusted proportional odds ratios: 2.55; 95% CI, 1.48-4.38 and 4.45; 95% CI, 1.75-11.27, respectively). CONCLUSIONS AND RELEVANCE: Water pipe tobacco smoking and the use of snus independently predicted the onset of cigarette smoking and current cigarette smoking at follow-up. Comprehensive Food and Drug Administration regulation of these tobacco products may limit their appeal to youth and curb the onset of cigarette smoking. Descriptors Links Book Title Database Publisher Data Source Authors Soneji,S., Sargent,J.D., Tanski,S.E., Primack,B.A. Original/Translated Title URL Date of Electronic PMCID PMC4388043 Editors
Effectiveness of different Web-based interventions to prepare co-smokers of cigarettes and cannabis for double cessation: a three-arm randomized controlled trial	2014	Swiss Research Institute for Public Health and Addiction ISGF, University of Zurich, Zurich, Switzerland. julia.becker@isgf.uzh.ch.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Journal of medical Internet research Periodical, Abbrev. J.Med.Internet Res. Pub Date Free Form 5-Dec Volume 16 Issue 12 Start Page e273 Other Pages Notes LR: 20151028; ISRCTN/ISRCTN56326375; JID: 100959882; OID: NLM: PMC4275498; OTO: NOTNLM; 2014/01/13 [received]; 2014/10/25 [accepted]; 2014/09/30 [revised]; epublish Place of Publication Canada ISSN/ISBN 1438-8871; 1438-8871 Accession Number PMID: 25486674 Language eng SubFile Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't; IM DOI 10.2196/jmir.3246 [doi] Output Language Unknown(0) PMID 25486674 Abstract BACKGROUND: The relationship between tobacco and cannabis use is strong. When co-smokers try to quit only one substance, this relationship often leads to a substitution effect, that is, the increased use of the remaining substance. Stopping the use of both substances simultaneously is therefore a reasonable strategy, but co-smokers rarely report feeling ready for simultaneous cessation. Thus, the question of how co-smokers can be motivated to attempt a simultaneous cessation has arisen. To reach as many co-smokers as possible, we developed brief Web-based interventions aimed at enhancing the readiness to simultaneously quit tobacco and cannabis use. OBJECTIVE: Our aim was to analyze the efficacy of three different Web-based interventions designed to enhance co-smokers' readiness to stop tobacco and cannabis use simultaneously. METHODS: Within a randomized trial, three brief Web-based and fully automated interventions were compared. The first intervention combined the assessment of cigarette dependence and problematic cannabis use with personalized, normative feedback. The second intervention was based on principles of motivational interviewing. As an active psychoeducational control group, the third intervention merely provided information on tobacco, cannabis, and the co-use of the two substances. The readiness to quit tobacco and cannabis simultaneously was measured before and after the intervention (both online) and 8 weeks later (online or over the phone). Secondary outcomes included the frequency of cigarette and cannabis use, as measured at baseline and after 8 weeks. RESULTS: A total of 2467 website users were assessed for eligibility based on their self-reported tobacco and cannabis co-use, and 325 participants were ultimately randomized and analyzed. For the post-intervention assessment, generalized estimating equations revealed a significant increase in the readiness to quit tobacco and cannabis in the total sample (B=.33, 95% CI 0.10-0.56, P=.006). However, this effect was not significant for the comparison between baseline and the 8-week follow-up assessment (P=.69). Furthermore, no differential effects between the interventions were found, nor were any significant intervention or time effects found on the frequency of tobacco or cannabis use. CONCLUSIONS: In the new field of dual interventions for co-smokers of tobacco and cannabis, Web-based interventions can increase the short-term readiness to quit tobacco and cannabis simultaneously. The studied personalized techniques were no more effective than was psychoeducation. The analyzed brief interventions did not change the secondary outcomes, that is the frequency of tobacco and cannabis use. TRIAL REGISTRATION: International Standard Randomized Controlled Trial Number (ISRCTN): 56326375; http://www.isrctn.com/ISRCTN56326375 (Archived by WebCite at http://www.webcitation.org/6UUWBh8u0). Descriptors Links Book Title Database Publisher Data Source Authors Becker,J., Haug,S., Sullivan,R., Schaub,M.P. Original/Translated Title URL Date of Electronic 20141205 PMCID PMC4275498 Editors
Effects of respiratory muscle and endurance training using an individualized training device on the pulmonary function and exercise capacity in stroke patients	2014	Department of Physical Therapy, Sahmyook University, Seoul, Korea.; Department of Physical Therapy, Sahmyook University, Seoul, Korea.; Department of Physical Therapy, Sahmyook University, Seoul, Korea.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Medical science monitor : international medical journal of experimental and clinical research Periodical, Abbrev. Med.Sci.Monit. Pub Date Free Form 5-Dec Volume 20 Issue Start Page 2543 Other Pages 2549 Notes LR: 20151028; JID: 9609063; OID: NLM: PMC4266259; epublish Place of Publication United States ISSN/ISBN 1643-3750; 1234-1010 Accession Number PMID: 25488849 Language eng SubFile Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't; IM DOI 10.12659/MSM.891112 [doi] Output Language Unknown(0) PMID 25488849 Abstract BACKGROUND: Because respiratory muscle function plays a strong role in exercise capacity and cardiopulmonary response to exercise, systematic training and measurement of respiratory muscle function should be considered in stroke patients. The purpose of this study was to determine whether an individualized respiratory muscle training device combined with conventional physical therapy exercise can improve the pulmonary function and exercise capacity of stroke patients. MATERIAL AND METHODS: Twenty stroke patients were randomly assigned to an exercise group (n=10) or a control group (n=10). Over 4 weeks, each group participated in exercise training interventions 3 times per week. In each session, the control group received basic exercise treatments for 30 min, followed by an automated full-body workout for 20 min. The exercise group performed the same exercise regimen as the control group, as well as an additional respiratory muscle training regimen using a respiratory exercise device for 20 min. RESULTS: Pulmonary function of forced vital capacity (FVC), forced expiratory volume at 1 s (FEV1), FEV1/FVC, and peak expiratory flow (PEF) and exercise capacity of a 6-min walking test and Shortness of Breath Modified Borg Dyspnea Scale (SBMBDS) scores were assessed before and after the training. A significant intergroup difference was observed in the FVC, FEV1, PEF, 6MWT, and SBMBDS scores (p Descriptors Links Book Title Database Publisher Data Source Authors Kim,J., Park,J.H., Yim,J. Original/Translated Title URL Date of Electronic 20141205 PMCID PMC4266259 Editors
Role of Cigarette Smoke-Induced Aggresome Formation in Chronic Obstructive Pulmonary Disease-Emphysema Pathogenesis	2015	Departments of 1 Pediatric Respiratory Science and.; 2 Biomedical Engineering, the Johns Hopkins University School of Medicine, Baltimore, Maryland; and.; Departments of 1 Pediatric Respiratory Science and.; 2 Biomedical Engineering, the Johns Hopkins Uni	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full American journal of respiratory cell and molecular biology Periodical, Abbrev. Am.J.Respir.Cell Mol.Biol. Pub Date Free Form Aug Volume 53 Issue 2 Start Page 159 Other Pages 173 Notes GR: R01HL59410-04/HL/NHLBI NIH HHS/United States; GR: U54CA141868/CA/NCI NIH HHS/United States; JID: 8917225; 0 (Cell Cycle Proteins); 0 (P62 protein, human); 0 (RNA-Binding Proteins); 0 (Ubiquitinated Proteins); EC 3.6.1.- (Adenosine Triphosphatases); EC Place of Publication United States ISSN/ISBN 1535-4989; 1044-1549 Accession Number PMID: 25490051 Language eng SubFile Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; IM DOI 10.1165/rcmb.2014-0107OC [doi] Output Language Unknown(0) PMID 25490051 Abstract Cigarette smoke (CS) exposure is known to induce proteostasis imbalance that can initiate accumulation of ubiquitinated proteins. Therefore, the primary goal of this study was to determine if first- and secondhand CS induces localization of ubiquitinated proteins in perinuclear spaces as aggresome bodies. Furthermore, we sought to determine the mechanism by which smoke-induced aggresome formation contributes to chronic obstructive pulmonary disease (COPD)-emphysema pathogenesis. Hence, Beas2b cells were treated with CS extract (CSE) for in vitro experimental analysis of CS-induced aggresome formation by immunoblotting, microscopy, and reporter assays, whereas chronic CS-exposed murine model and human COPD-emphysema lung tissues were used for validation. In preliminary analysis, we observed a significant (P Descriptors Links Book Title Database Publisher Data Source Authors Tran,I., Ji,C., Ni,I., Min,T., Tang,D., Vij,N. Original/Translated Title URL Date of Electronic PMCID Editors
Hookah pipes are associated with young people starting smoking, study finds	2014	London.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full BMJ (Clinical research ed.) Periodical, Abbrev. BMJ Pub Date Free Form 8-Dec Volume 349 Issue Start Page g7546 Other Pages Notes JID: 8900488; epublish Place of Publication England ISSN/ISBN 1756-1833; 0959-535X Accession Number PMID: 25491109 Language eng SubFile News; AIM; IM DOI 10.1136/bmj.g7546 [doi] Output Language Unknown(0) PMID 25491109 Abstract Descriptors Links Book Title Database Publisher Data Source Authors Wise,J. Original/Translated Title URL Date of Electronic 20141208 PMCID Editors
Waterpipe tobacco smoking: what is the evidence that it supports nicotine/tobacco dependence?	2015	Center for Clinical and Translational Research and Center for the Study of Tobacco Products, Virginia Commonwealth University, Richmond, Virginia, USA.; Department of Psychology, Center for the Study of Tobacco Products, Virginia Commonwealth University,	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Tobacco control Periodical, Abbrev. Tob.Control Pub Date Free Form Mar Volume 24 Suppl 1 Issue Start Page i44 Other Pages i53 Notes LR: 20150618; CI: Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.; GR: P50 DA036105/DA/NIDA NIH HHS/United States; GR: P Place of Publication England ISSN/ISBN 1468-3318; 0964-4563 Accession Number PMID: 25492935 Language eng SubFile Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review; IM DOI 10.1136/tobaccocontrol-2014-051910 [doi] Output Language Unknown(0) PMID 25492935 Abstract OBJECTIVE: Waterpipe tobacco smoking (WTS) involves passing tobacco smoke through water prior to inhalation, and has spread worldwide. This spread becomes a public health concern if it is associated with tobacco-caused disease and if WTS supports tobacco/nicotine dependence. A growing literature demonstrates that WTS is associated with disability, disease and death. This narrative review examines if WTS supports nicotine/tobacco dependence, and is intended to help guide tobacco control efforts worldwide. DATA SOURCES: PUBMED search using: (("waterpipe" or "narghile" or "arghile" or "shisha" or "goza" or "narkeela" or "hookah" or "hubble bubble")) AND ("dependence" or "addiction"). STUDY SELECTION: Excluded were articles not in English, without original data, and that were not topic-related. Thirty-two articles were included with others identified by inspecting reference lists and other sources. DATA SYNTHESIS: WTS and the delivery of the dependence-producing drug nicotine were examined, and then the extent to which the articles addressed WTS-induced nicotine/dependence explicitly, as well as implicitly with reference to criteria for dependence outlined by the WHO. CONCLUSIONS: WTS supports nicotine/tobacco dependence because it is associated with nicotine delivery, and because some smokers experience withdrawal when they abstain from waterpipe, alter their behaviour in order to access a waterpipe and have difficulty quitting, even when motivated to do so. There is a strong need to support research investigating measurement of WTS-induced tobacco dependence, to inform the public of the risks of WTS, which include dependence, disability, disease and death, and to include WTS in the same public health policies that address tobacco cigarettes. Descriptors Links Book Title Database Publisher Data Source Authors Aboaziza,E., Eissenberg,T. Original/Translated Title URL Date of Electronic 20141209 PMCID PMC4345797 Editors
Clearing the air: improving smoke-free policy compliance at the national oncology hospital in Armenia	2014	School of Public Health, American University of Armenia, Yerevan, Armenia. nmovsesi@aua.am.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full BMC cancer Periodical, Abbrev. BMC Cancer Pub Date Free Form 13-Dec Volume 14 Issue Start Page 943 Other Pages 2407-14-943 Notes LR: 20151028; JID: 100967800; 0 (Tobacco Smoke Pollution); OID: NLM: PMC4320561; 2014/08/04 [received]; 2014/12/08 [accepted]; 2014/12/13 [aheadofprint]; epublish Place of Publication England ISSN/ISBN 1471-2407; 1471-2407 Accession Number PMID: 25495431 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1186/1471-2407-14-943 [doi] Output Language Unknown(0) PMID 25495431 Abstract BACKGROUND: Smoke-free policies shown to reduce population exposure to secondhand smoke (SHS) are the norm in hospitals in many countries around the world. Armenia, a transition economy in the South Caucasus, has one of the highest male smoking rates in the European region. Although smoking in healthcare facilities has been banned since 2005, compliance with this ban has been poor due to lack of implementation and enforcement mechanisms and social acceptability of smoking. The study aimed to develop and test a model intervention to address the lack of compliance with the de jure smoking ban. The national oncology hospital was chosen as the intervention site. METHODS: This study used employee surveys and objective measurements of respirable particles (PM2.5) and air nicotine as markers of indoor air pollution before and after the intervention. The intervention developed in partnership with the hospital staff included an awareness campaign on SHS hazards, creation of no-smoking environment and building institutional capacity through training of nursing personnel on basics of tobacco control. The survey analysis included paired t-test and McNemar's test. The log-transformed air nicotine and PM2.5 data were analyzed using paired t-test. RESULTS: The survey showed significant improvement in the perceived quality of indoor air, reduced worksite exposure to SHS and increased employees' awareness of the smoke-free policy. The number of employees reporting compliance with the hospital smoke-free policy increased from 36.0% to 71.9% (p Descriptors Links Book Title Database Publisher Data Source Authors Movsisyan,N.K., Petrosyan,V., Harutyunyan,A., Petrosyan,D., Stillman,F. Original/Translated Title URL Date of Electronic 20141213 PMCID PMC4320561 Editors
MicroRNA Expression can be a Promising Strategy for the Detection of Barrett's Esophagus: A Pilot Study	2014	1] Division of Gastroenterology and Hepatology, Veterans Affairs Medical Center, Kansas City, Missouri, USA [2] University of Kansas Medical Center, Kansas City, Kansas, USA [3] Kansas Cancer Institute, Kansas City, Kansas, USA.; Department of Molecular a	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Clinical and translational gastroenterology Periodical, Abbrev. Clin.Transl.Gastroenterol. Pub Date Free Form 11-Dec Volume 5 Issue Start Page e65 Other Pages Notes LR: 20141231; JID: 101532142; OID: NLM: PMC4274369; 2014/08/15 [received]; 2014/11/10 [accepted]; epublish Place of Publication United States ISSN/ISBN 2155-384X Accession Number PMID: 25502391 Language eng SubFile Journal Article DOI 10.1038/ctg.2014.17 [doi] Output Language Unknown(0) PMID 25502391 Abstract OBJECTIVES: Patient outcomes for esophageal adenocarcinoma (EAC) have not improved despite huge advances in endoscopic therapy because cancers are being diagnosed late. Barrett's esophagus (BE) is the primary precursor lesion for EAC, and thus the non-endoscopic molecular diagnosis of BE can be an important approach to improve EAC outcomes if robust biomarkers for timely diagnosis are identified. MicroRNAs (miRNAs) are tissue-specific novel biomarkers that regulate gene expression and may satisfy this requirement. METHODS: Patients with gastroesophageal reflux disease (GERD) and BE were selected from an ongoing tissue and serum repository. BE was defined by the presence of intestinal metaplasia. Previously published miRNA sequencing profiles of GERD and BE patients allowed us to select three miRNAs, miR-192-5p, -215-5p, and -194-5p, for further testing in a discovery cohort and an independent validation cohort. Receiver operating curves were generated to calculate the diagnostic accuracy of these miRNAs for BE diagnosis. To test specificity, the miRNA signature was compared with those of the gastric cardia epithelium and the non-intestinal-type columnar epithelium (another definition of BE). In addition, to gain insights into BE origin (intestinal vs non-intestinal), global BE miRNA profiles were compared with the published miRNA profiles of other columnar epithelia in the gastrointestinal tract, that is, normal stomach and small and large intestine. RESULTS: The discovery cohort included 67 white male patients (40 with GERD and 27 with BE). The validation cohort included 28 patients (19 with GERD and 11 with BE). In the discovery cohort, the sensitivity, specificity and area under the curve (AUC) of the three mRNAs for BE diagnosis were 92-100%, 94-95%, and 0.96-0.97, respectively. During validation, the sensitivity and specificity of miRNAs for BE diagnosis were as follows: miR-192-5p, 92% and 94%, AUC 0.94 (0.80-0.99, P=0.0004); miR-215-5p, 100% and 94%, AUC 0.98 (0.84-1, P=0.0004); and miR-194-5p, 91% and 94%, AUC 0.96 (0.80-0.99, P=0.0001), respectively. The tested miRNAs identified all BE patients in both the discovery and the validation cohorts. When compared with non intestinal-type columnar and gastric cardia epithelia, the miRNA signature was specific to the intestinal-type columnar epithelium. Comparisons of BE miRNA sequencing data to published data sets for the normal stomach, small intestine and large intestine confirmed that two of the three miRNAs (miR-215-5p and -194-5p) were specific to the intestinal-type epithelium. CONCLUSIONS: MicroRNAs are highly accurate for detecting intestinal-type BE epithelia and should be tested further for the non-endoscopic molecular diagnosis of BE. Descriptors Links Book Title Database Publisher Data Source Authors Bansal,A., Hong,X., Lee,I.H., Krishnadath,K.K., Mathur,S.C., Gunewardena,S., Rastogi,A., Sharma,P., Christenson,L.K. Original/Translated Title URL Date of Electronic 20141211 PMCID PMC4274369 Editors

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