WTS database

Title	Pub Year	Author	SearchLink Sort descending
Innovative sol-gel coatings for solid-phase microextraction. Development of fibers for the determination of polycyclic aromatic hydrocarbons at trace level in water	2008	Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Universita degli Studi di Parma, Viale Usberti 17/A, Parma, Italy. federica.bianchi@unipr.it	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Journal of chromatography.A Periodical, Abbrev. J.Chromatogr.A Pub Date Free Form 4-Jul Volume 1196-1197 Issue Start Page 15 Other Pages 22 Notes LR: 20090115; JID: 9318488; 0 (Polycyclic Hydrocarbons, Aromatic); 0 (Water Pollutants, Chemical); 2007/12/10 [received]; 2008/01/14 [revised]; 2008/01/15 [accepted]; 2008/01/20 [aheadofprint]; ppublish Place of Publication Netherlands ISSN/ISBN 0021-9673; 0021-9673 Accession Number PMID: 18258246 Language eng SubFile Journal Article; IM DOI 10.1016/j.chroma.2008.01.036 [doi] Output Language Unknown(0) PMID 18258246 Abstract Innovative solid-phase microextraction coatings synthesized by sol-gel technology were developed for the determination of environmental pollutants, i.e. polycyclic aromatic hydrocarbons at trace level in water. The fibers obtained, based on the use of (3-aminopropyl)triethoxysilane and diethoxydiphenylsilane were characterised in terms of film thickness, porosity, thermal stability and pH resistance. An excellent thermal stability was obtained in the case of diethoxydiphenylsilane-based coating, with a negligible weight loss until 400 degrees C. A very good fiber-to-fiber and batch-to-batch repeatability was also obtained with RSD lower than 6% using a mixture of aromatic hydrocarbons in the low ng/l range. Finally, the capabilities of the developed coating for sampling polycyclic aromatic hydrocarbons in water at trace levels were proved obtaining limits of detection about twofold lower than those achieved by using the commercially available polydimethylsiloxane 7 microm fiber. Descriptors Gas Chromatography-Mass Spectrometry, Microscopy, Electron, Scanning, Molecular Structure, Polycyclic Hydrocarbons, Aromatic/analysis/chemistry, Reproducibility of Results, Solid Phase Microextraction/methods, Water Pollutants, Chemical/analysis/chemistry Links Book Title Database Publisher Data Source Authors Bianchi,F., Bisceglie,F., Careri,M., Di Berardino,S., Mangia,A., Musci,M. Original/Translated Title URL Date of Electronic 20080120 PMCID Editors
Effects of phosphoric acid pretreatment and substitution of bonding resin on bonding effectiveness of self-etching systems to enamel	2007	Department of Dental Materials and Operative Dentistry, University of Oeste de Santa Catarina, Joacaba, SC, Brazil.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full The journal of adhesive dentistry Periodical, Abbrev. J.Adhes.Dent. Pub Date Free Form Dec Volume 9 Issue 6 Start Page 537 Other Pages 545 Notes LR: 20121115; JID: 100888552; 0 (Clearfil SE Bond); 0 (Composite Resins); 0 (Dentin-Bonding Agents); 0 (Filtek Z250); 0 (Phosphoric Acids); 0 (Prompt L-Pop); 0 (Resin Cements); 0 (Scotchbond Multi-Purpose); 0 (Tyrian SPE); E4GA8884NN (phosphoric acid); pp Place of Publication England ISSN/ISBN 1461-5185; 1461-5185 Accession Number PMID: 18265711 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; D DOI Output Language Unknown(0) PMID 18265711 Abstract PURPOSE: To examine the etching efficacy of three self-etching systems to unconditioned enamel and to phosphoric-acid conditioned enamel using scanning electron microscopy (SEM), and to investigate the microtensile bond strength (muTBS) of three self-etching adhesives to unconditioned enamel used according to the manufacturers' instructions, on phosphoric-acid conditioned enamel, and when their different adhesives were replaced with a control resin after etching. MATERIALS AND METHODS: Clearfil SE Bond (CSE), Tyrian SPE/One-Step Plus (TY), and Adper Prompt L-Pop (AD) were employed. Scotchbond Multi-Purpose Plus (SBMP), an etch-and-rinse adhesive, was used as control. For muTBS evaluation, the buccal or lingual mid-coronal enamel of 30 molars was used. In the first group, self-etching adhesives were applied conventionally, according to manufacturers' directions (group C). A second group of specimens was etched with 35% phosphoric acid before application of the self-etching adhesives (group PA), and the third group of specimens was etched with the self-etching adhesives, rinsed, but bonded using a control bonding resin (group CR). Following restoration with Z250, they were sectioned into beams (0.8 mm2), and stressed to failure. Data were subjected to two-way ANOVA and Tukey's test. The etching efficacy of the self-etching adhesives, phosphoric acid, and prior phosphoric acid + SE application was evaluated under SEM. RESULTS: All self-etching adhesives applied as recommended by the manufacturers showed lower muTBS values than those obtained in PA and CR groups, although this difference was only significant for TY and AD. The means of all self-etching adhesives under PA and CR groups were similar to SBMP. The enamel conditioned with self-etching adhesive showed a less distinct pattern. CSE exhibited the mildest etching pattern. All self-etching adhesives applied after phosphoric acid exhibited a more retentive etching pattern. CONCLUSION: The bond strength values of low-pH self-etching adhesives can be improved by the adjunctive use of phosphoric acid or replacement of the bonding resin. Descriptors Acid Etching, Dental, Composite Resins/chemistry, Dental Bonding, Dental Enamel/ultrastructure, Dentin-Bonding Agents/chemistry, Humans, Materials Testing, Microscopy, Electron, Scanning, Phosphoric Acids/chemistry, Resin Cements/chemistry, Stress, Mechanical, Surface Properties, Tensile Strength Links Book Title Database Publisher Data Source Authors Rotta,M., Bresciani,P., Moura,S. K., Grande,R. H., Hilgert,L. A., Baratieri,L. N., Loguercio,A. D., Reis,A. Original/Translated Title URL Date of Electronic PMCID Editors
Socio-economic variations in tobacco consumption, intention to quit and self-efficacy to quit among male smokers in Thailand and Malaysia: results from the International Tobacco Control-South-East Asia (ITC-SEA) survey	2008	Department of Health Promotion, Social and Behavioral Health, College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska, USA. msiahpush@unmc.edu	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Addiction (Abingdon, England) Periodical, Abbrev. Addiction Pub Date Free Form Mar Volume 103 Issue 3 Start Page 502 Other Pages 508 Notes LR: 20150421; GR: C312/A3726/Cancer Research UK/United Kingdom; GR: P50 CA111236/CA/NCI NIH HHS/United States; GR: P50 CA111236/CA/NCI NIH HHS/United States; GR: R01 CA 100362/CA/NCI NIH HHS/United States; GR: R01 CA100362/CA/NCI NIH HHS/United States; JI Place of Publication England ISSN/ISBN 0965-2140; 0965-2140 Accession Number PMID: 18269370 Language eng SubFile Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; IM DOI 10.1111/j.1360-0443.2007.02113.x [doi] Output Language Unknown(0) PMID 18269370 Abstract Aim To examine the association of socio-economic position (education, income and employment status) with cigarette consumption, intention to quit and self-efficacy to quit among male smokers in Thailand and Malaysia. Design and setting The data were based on a survey of adult smokers conducted in early 2005 in Thailand and Malaysia as part of the International Tobacco Control-South-East Asia (ITC-SEA) project. Participants A total of 1846 men in Thailand and 1906 men in Malaysia. Measurement Participants were asked questions on daily cigarette consumption, intention to quit and self-efficacy to quit in face-to-face interviews. Findings Analyses were based on multivariate regression models that adjusted for all three socio-economic indicators. In Thailand, higher level of education was associated strongly with not having self-efficacy, associated weakly with having an intention to quit and was not associated with cigarette consumption. Higher income was associated strongly with having self-efficacy, associated weakly with high cigarette consumption and was not associated with having an intention to quit. Being employed was associated strongly with having an intention to quit and was not associated with cigarette consumption or self-efficacy. In Malaysia, higher level of education was not associated with any of the outcomes. Higher income was associated strongly with having self-efficacy, and was not associated with the other outcomes. Being employed was associated moderately with higher cigarette consumption and was not associated with the other outcomes. Conclusion Socio-economic and cultural conditions, as well as tobacco control policies and tobacco industry activities, shape the determinants of smoking behaviour and beliefs. Existing knowledge from high-income countries about disparities in smoking should not be generalized readily to other countries. Descriptors Adolescent, Adult, Aged, Health Surveys, Humans, Intention, Malaysia/epidemiology, Male, Middle Aged, Self Efficacy, Smoking/epidemiology/psychology, Smoking Cessation/psychology, Socioeconomic Factors, Thailand/epidemiology Links Book Title Database Publisher Data Source Authors Siahpush,M., Borland,R., Yong,H. H., Kin,F., Sirirassamee,B. Original/Translated Title URL Date of Electronic PMCID Editors
Concentrations of bisphenol a, bisphenol a diglycidyl ether, and their derivatives in canned foods in Japanese markets	2008	Nihon Waters KK, Katokichi Shin-Osaka Building, Osaka, Japan.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Journal of Agricultural and Food Chemistry Periodical, Abbrev. J.Agric.Food Chem. Pub Date Free Form 26-Mar Volume 56 Issue 6 Start Page 2041 Other Pages 2047 Notes LR: 20131121; JID: 0374755; 0 (Benzhydryl Compounds); 0 (Epoxy Compounds); 0 (Phenols); F3XRM1NX4H (2,2-bis(4-glycidyloxyphenyl)propane); MLT3645I99 (bisphenol A); 2008/02/20 [aheadofprint]; ppublish Place of Publication United States ISSN/ISBN 0021-8561; 0021-8561 Accession Number PMID: 18284199 Language eng SubFile Journal Article; IM DOI 10.1021/jf073106n [doi] Output Language Unknown(0) PMID 18284199 Abstract Bisphenol A (BPA), bisphenol A diglycidyl ether (BADGE), and their derivatives in 38 canned foods sold in Japan were measured using high-performance liquid chromatography-mass spectrometry (LC-MS) and LC-tandem mass spectrometry (LC-MS/MS). BPA, BADGE, BADGE.2H 2O, BADGE.HCl.H2O, BADGE.HCl, and BADGE.2HCl were 0-235.4, 0-3.4, 0-247.2, 0.2-196.4, 0-3.0, and 0-25.7 ng/g, respectively, which did not exceed acceptable daily intake for BPA and specific migration limit for BADGEs. BADGE was degraded by 58, 100, 46, and 58% in water (pH 7), 0.01 N HCl (pH 2), 0.01 N NaCl (pH 6.8), and 0.01 N NaCl with acetic acid (pH 2.5), respectively, when it was allowed to stand at 120 degrees C for 30 min. The prominent derivatives formed were BADGE.2H 2O and BADGE.HCl.H2O, which was formed not only in BADGE with added HCl but also in that with NaCl. Acetic acid accelerated the formation of both BADGE.2H2O and BADGE.HCl.H2O in NaCl. No BPA was detected in any simulation samples started from BADGE. The results suggest that BPA and BADGE are independently leached into canned foods and that BADGE is easily changed to more stable compounds such as BADGE.2H2O and BADGE.HCl.H2O by sterilization. Descriptors Benzhydryl Compounds, Chromatography, High Pressure Liquid, Epoxy Compounds/analysis, Food Handling/methods, Food Packaging, Food Preservation, Hot Temperature, Japan, Mass Spectrometry, Phenols/analysis, Sterilization Links Book Title Database Publisher Data Source Authors Yonekubo,J., Hayakawa,K., Sajiki,J. Original/Translated Title URL Date of Electronic 20080220 PMCID Editors
Absence of amphotericin B-tolerant persister cells in biofilms of some Candida species	2008	Division of Infection and Immunity, Faculty of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, United Kingdom.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Antimicrobial Agents and Chemotherapy Periodical, Abbrev. Antimicrob.Agents Chemother. Pub Date Free Form May Volume 52 Issue 5 Start Page 1884 Other Pages 1887 Notes LR: 20140904; JID: 0315061; 0 (Antifungal Agents); 7XU7A7DROE (Amphotericin B); OID: NLM: PMC2346666; 2008/02/19 [aheadofprint]; ppublish Place of Publication United States ISSN/ISBN 1098-6596; 0066-4804 Accession Number PMID: 18285487 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1128/AAC.01473-07 [doi] Output Language Unknown(0) PMID 18285487 Abstract Biofilms and planktonic cells of five Candida species were surveyed for the presence of persister (drug-tolerant) cell populations after exposure to amphotericin B. None of the planktonic cultures (exponential or stationary phase) contained persister cells. However, persisters were found in biofilms of one of two strains of Candida albicans tested and in biofilms of Candida krusei and Candida parapsilosis, but not in biofilms of Candida glabrata or Candida tropicalis. These results suggest that persister cells cannot solely account for drug resistance in Candida biofilms. Descriptors Amphotericin B/pharmacology, Antifungal Agents/pharmacology, Biofilms/drug effects/growth & development, Candida/cytology/drug effects/growth & development, Drug Resistance, Fungal, Species Specificity Links Book Title Database Publisher Data Source Authors Al-Dhaheri,R. S., Douglas,L. J. Original/Translated Title URL Date of Electronic 20080219 PMCID PMC2346666 Editors
Revealing source signatures in ambient BTEX concentrations	2008	Environmental, Water and Agricultural Engineering Department, Faculty of Civil and Environmental Engineering, Israel Institute of Technology, Technion, Haifa 32000, Israel.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Environmental pollution (Barking, Essex : 1987) Periodical, Abbrev. Environ.Pollut. Pub Date Free Form Nov Volume 156 Issue 2 Start Page 553 Other Pages 562 Notes LR: 20131121; JID: 8804476; 0 (Air Pollutants); 0 (Benzene Derivatives); 0 (Industrial Waste); 0 (Oxidants, Photochemical); 0 (Vehicle Emissions); 0 (Xylenes); 3FPU23BG52 (Toluene); J64922108F (Benzene); L5I45M5G0O (ethylbenzene); S7G510RUBH (Nitrogen Dio Place of Publication England ISSN/ISBN 1873-6424; 0269-7491 Accession Number PMID: 18289752 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1016/j.envpol.2008.01.016 [doi] Output Language Unknown(0) PMID 18289752 Abstract Management of ambient concentrations of Volatile Organic Compounds (VOCs) is essential for maintaining low ozone levels in urban areas where its formation is under a VOC-limited regime. The significant decrease in traffic-induced VOC emissions in many developed countries resulted in relatively comparable shares of traffic and non-traffic VOC emissions in urban airsheds. A key step for urban air quality management is allocating ambient VOC concentrations to their pertinent sources. This study presents an approach that can aid in identifying sources that contribute to observed BTEX concentrations in areas characterized by low BTEX concentrations, where traditional source apportionment techniques are not useful. Analysis of seasonal and diurnal variations of ambient BTEX concentrations from two monitoring stations located in distinct areas reveal the possibility to identify source categories. Specifically, the varying oxidation rates of airborne BTEX compounds are used to allocate contributions of traffic emissions and evaporative sources to observed BTEX concentrations. Descriptors Air Pollutants/analysis, Benzene/analysis, Benzene Derivatives/analysis, Environmental Monitoring/methods, Industrial Waste, Israel, Nitrogen Dioxide/analysis, Oxidants, Photochemical/analysis, Particle Size, Seasons, Time, Toluene/analysis, Vehicle Emissions, Xylenes/analysis Links Book Title Database Publisher Data Source Authors Zalel,A., Yuval, Broday,D. M. Original/Translated Title URL Date of Electronic 20080304 PMCID Editors
Migration of BADGE (bisphenol A diglycidyl-ether) and BFDGE (bisphenol F diglycidyl-ether) in canned seafood	2008	ANFACO-CECOPESCA, Col Univ 16, Vigo, Spain. agcabado@anfaco.es	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association Periodical, Abbrev. Food Chem.Toxicol. Pub Date Free Form May Volume 46 Issue 5 Start Page 1674 Other Pages 1680 Notes LR: 20131121; JID: 8207483; 0 (Benzhydryl Compounds); 0 (Carcinogens); 0 (Epoxy Compounds); 0 (Indicators and Reagents); 0 (Lipids); 0 (bisphenol F diglycidyl ether); F3XRM1NX4H (2,2-bis(4-glycidyloxyphenyl)propane); 2007/01/11 [received]; 2007/08/29 [rev Place of Publication England ISSN/ISBN 0278-6915; 0278-6915 Accession Number PMID: 18289761 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1016/j.fct.2008.01.006 [doi] Output Language Unknown(0) PMID 18289761 Abstract Migration of potentially toxic materials used for the lining of commercial can goods remains an important issue, especially with respect to certain types of processed foods. Seafood is one type where more information is needed with respect to other ingredients used for adding value to fishery products. Most cans are internally coated with starters of resins such as bisphenol A diglycidyl-ether (BADGE) and bisphenol F diglycidyl-ether (BFDGE), both considered as toxic compounds. Several seafood products, sardines, tuna fish, mackerel, mussels, cod and mackerel eggs, were manufactured in different conditions changing covering sauce, time and temperature of storage and heat-treated for sterilization in cans. Migration kinetics of BADGE and BFDGE from varnish into canned products were evaluated by HPLC in 70 samples after 6, 12 or 18 months of storage. Results showed that there is no migration of BADGE in tuna fish, sardines, mussels or cod. However, migration of BFDGE occurs in all species, in a storage time-dependent way and content of fat, although migration of these compounds is not affected by sterilization conditions. All samples analyzed presented values lower than 9 mg BADGE/kg net product without exceeding European limits. However, concerning BFDGE migration, European legislation does not allow the use and/or the presence of BFDGE. Main migration takes place in mackerel reaching the highest values, 0.74 mg BFDGE/kg and 0.34 mg BADGE/kg net product, in red pepper sauce. Descriptors Animals, Benzhydryl Compounds, Bivalvia, Carcinogens/analysis, Chromatography, High Pressure Liquid, Epoxy Compounds/analysis, Food Analysis, Food Preservation, Gadus morhua, Indicators and Reagents, Lipids/analysis, Perciformes, Reproducibility of Results, Seafood/analysis, Sterilization, Temperature, Time Factors, Tuna Links Book Title Database Publisher Data Source Authors Cabado,A. G., Aldea,S., Porro,C., Ojea,G., Lago,J., Sobrado,C., Vieites,J. M. Original/Translated Title URL Date of Electronic 20080112 PMCID Editors
A 32-country comparison of tobacco smoke derived particle levels in indoor public places	2008	Roswell Park Cancer Institute, Department of Health Behavior, Elm and Carlton Streets, Buffalo, NY 14263, USA. andrew.hyland@roswellpark.org	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Tobacco control Periodical, Abbrev. Tob.Control Pub Date Free Form Jun Volume 17 Issue 3 Start Page 159 Other Pages 165 Notes LR: 20141120; GR: P50 CA111236/CA/NCI NIH HHS/United States; JID: 9209612; 0 (Tobacco Smoke Pollution); 2008/02/26 [aheadofprint]; ppublish Place of Publication England ISSN/ISBN 1468-3318; 0964-4563 Accession Number PMID: 18303089 Language eng SubFile Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; IM DOI 10.1136/tc.2007.020479 [doi] Output Language Unknown(0) PMID 18303089 Abstract OBJECTIVE: To compare tobacco smoke-derived particulate levels in transportation and hospitality venues with and without smoking in 32 countries using a standardised measurement protocol. METHODS: The TSI SidePak AM510 Personal Aerosol Monitor was used to measure the concentration of particulate matter less than 2.5 microns in diameter (PM(2.5)) in 1822 bars, restaurants, retail outlets, airports and other workplaces in 32 geographically dispersed countries between 2003 and 2007. RESULTS: Geometric mean PM(2.5) levels were highest in Syria (372 microg/m(3)), Romania (366 microg/m(3)) and Lebanon (346 microg/m(3)), while they were lowest in the three countries that have nationwide laws prohibiting smoking in indoor public places (Ireland at 22 microg/m(3), Uruguay at 18 microg/m(3) and New Zealand at 8 microg/m(3)). On average, the PM(2.5) levels in places where smoking was observed was 8.9 times greater (95% CI 8.0 to 10) than levels in places where smoking was not observed. CONCLUSIONS: Levels of indoor fine particle air pollution in places where smoking is observed are typically greater than levels that the World Health Organization and US Environmental Protection Agency have concluded are harmful to human health. Descriptors Air Pollution, Indoor/analysis, Global Health, Humans, Public Facilities/statistics & numerical data, Smoking/epidemiology/legislation & jurisprudence/prevention & control, Tobacco Smoke Pollution/adverse effects/analysis, World Health Organization Links Book Title Database Publisher Data Source Authors Hyland,A., Travers,M. J., Dresler,C., Higbee,C., Cummings,K. M. Original/Translated Title URL Date of Electronic 20080226 PMCID Editors
Passive versus active parental permission: implications for the ability of school-based depression screening to reach youth at risk	2008	Psychology, PGSP-Stanford Consortium, 247 San Carlos Ave, Redwood City, CA 94061, USA. mpcpsyd@stanford.edu	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full The Journal of school health Periodical, Abbrev. J.Sch.Health Pub Date Free Form Mar Volume 78 Issue 3 Start Page 157 Other Pages 64; quiz 184-6 Notes LR: 20151119; GR: R01 MH079402/MH/NIMH NIH HHS/United States; GR: R01 MH079402-01A2/MH/NIMH NIH HHS/United States; GR: R24 HD042828/HD/NICHD NIH HHS/United States; GR: R24 HD042828-10/HD/NICHD NIH HHS/United States; JID: 0376370; NIHMS99261; OID: NLM: NIH Place of Publication United States ISSN/ISBN 0022-4391; 0022-4391 Accession Number PMID: 18307611 Language eng SubFile Comparative Study; Journal Article; IM; N DOI 10.1111/j.1746-1561.2007.00278.x [doi] Output Language Unknown(0) PMID 18307611 Abstract BACKGROUND: Depression is prevalent among children and adolescents and often goes untreated with adverse effects on academic success and healthy development. Depression screening can facilitate early identification and timely referral to prevention and treatment programs. Conducting school-based emotional health screening, however, raises the controversial issue of how to obtain informed parental permission. METHODS: During implementation of a depression screening program in an urban school district in the Pacific Northwest, the district's parental permission protocol changed from passive (information provided to parents via a school mailer with parents having the option to actively decline their child's participation) to active (information provided to parents via a school mailer requiring the written permission of the parents for their child's participation). This change provided an opportunity to examine differences in participation under these 2 conditions. RESULTS: A total of 1533 students were enrolled in this program across both years. Compared to conditions of passive permission, participation was dramatically reduced when children were required to have written parental permission, dropping from 85% to 66% of eligible children. Furthermore, under conditions of active parental permission, participation decreased differentially among student subgroups with increased risk for depression. CONCLUSIONS: Successful implementation of school-based emotional health screening programs requires careful consideration of how to inform and obtain permission from parents. Descriptors Adult, Child, Child Behavior/psychology, Correspondence as Topic, Depression/diagnosis, Education, Continuing, Ethnic Groups, Female, Humans, Male, Mass Screening, Middle Aged, Parental Consent/psychology, Parental Notification, Parents/psychology, Patient Compliance/statistics & numerical data, School Health Services, Schools, Students/psychology, Surveys and Questionnaires, Urban Population, Washington Links Book Title Database Publisher Data Source Authors Chartier,M., Stoep,A. V., McCauley,E., Herting,J. R., Tracy,M., Lymp,J. Original/Translated Title URL Date of Electronic PMCID PMC2713664 Editors
Taxonomic structure and stability of the bacterial community in belgian sourdough ecosystems as assessed by culture and population fingerprinting	2008	Laboratory of Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium. ilse.scheirlinck@ugent.be	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Applied and Environmental Microbiology Periodical, Abbrev. Appl.Environ.Microbiol. Pub Date Free Form Apr Volume 74 Issue 8 Start Page 2414 Other Pages 2423 Notes LR: 20140904; GENBANK/AM901454; GENBANK/AM901455; GENBANK/AM901456; GENBANK/AM901457; GENBANK/AM901458; GENBANK/AM901459; GENBANK/AM901460; GENBANK/AM901461; GENBANK/AM901462; GENBANK/AM901463; GENBANK/AM901464; GENBANK/AM901465; GENBANK/AM901466; GENBANK Place of Publication United States ISSN/ISBN 1098-5336; 0099-2240 Accession Number PMID: 18310426 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1128/AEM.02771-07 [doi] Output Language Unknown(0) PMID 18310426 Abstract A total of 39 traditional sourdoughs were sampled at 11 bakeries located throughout Belgium which were visited twice with a 1-year interval. The taxonomic structure and stability of the bacterial communities occurring in these traditional sourdoughs were assessed using both culture-dependent and culture-independent methods. A total of 1,194 potential lactic acid bacterium (LAB) isolates were tentatively grouped and identified by repetitive element sequence-based PCR, followed by sequence-based identification using 16S rRNA and pheS genes from a selection of genotypically unique LAB isolates. In parallel, all samples were analyzed by denaturing gradient gel electrophoresis (DGGE) of V3-16S rRNA gene amplicons. In addition, extensive metabolite target analysis of more than 100 different compounds was performed. Both culturing and DGGE analysis showed that the species Lactobacillus sanfranciscensis, Lactobacillus paralimentarius, Lactobacillus plantarum, and Lactobacillus pontis dominated the LAB population of Belgian type I sourdoughs. In addition, DGGE band sequence analysis demonstrated the presence of Acetobacter sp. and a member of the Erwinia/Enterobacter/Pantoea group in some samples. Overall, the culture-dependent and culture-independent approaches each exhibited intrinsic limitations in assessing bacterial LAB diversity in Belgian sourdoughs. Irrespective of the LAB biodiversity, a large majority of the sugar and amino acid metabolites were detected in all sourdough samples. Principal component-based analysis of biodiversity and metabolic data revealed only little variation among the two samples of the sourdoughs produced at the same bakery. The rare cases of instability observed could generally be linked with variations in technological parameters or differences in detection capacity between culture-dependent and culture-independent approaches. Within a sampling interval of 1 year, this study reinforces previous observations that the bakery environment rather than the type or batch of flour largely determines the development of a stable LAB population in sourdoughs. Descriptors Acetobacter/chemistry/classification/genetics/isolation & purification, Amino Acids/analysis, Belgium, Biodiversity, Carbohydrates/analysis, DNA Fingerprinting, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Ecosystem, Electrophoresis, Polyacrylamide Gel, Enterobacteriaceae/chemistry/classification/genetics/isolation & purification, Flour/microbiology, Food Microbiology, Interspersed Repetitive Sequences, Lactobacillus/chemistry/classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Denaturation, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA Links Book Title Database Publisher Data Source Authors Scheirlinck,I., Van der Meulen,R., Van Schoor,A., Vancanneyt,M., De Vuyst,L., Vandamme,P., Huys,G. Original/Translated Title URL Date of Electronic 20080229 PMCID PMC2293155 Editors

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