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Wheeze, eczema and rhinitis in 6-7 year old Irish schoolchildren 2003 Department of Paediatrics, Our Lady of Lourdes Hospital, Drogheda, Co. Louth.
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Journal Article
Periodical, Full
Irish medical journal
Periodical, Abbrev.
Ir.Med.J.
Pub Date Free Form
Apr
Volume
96
Issue
4
Start Page
102
Other Pages
104
Notes
LR: 20151119; JID: 0430275; 0 (Tobacco Smoke Pollution); ppublish
Place of Publication
Ireland
ISSN/ISBN
0332-3102; 0332-3102
Accession Number
PMID: 12793469
Language
eng
SubFile
Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
12793469
Abstract
The aim of this study was to determine the prevalence of wheeze, eczema and rhinitis in 6-7 year old schoolchildren in Louth and Meath and assess their risk factors. The ISAAC (International Study for Asthma and Allergies in Childhood) questionnaire was used. The response rate was 64.1% (n = 1899). The prevalence rates for wheeze, eczema and rhinitis were 17.4%, 11.2% and 20.2% respectively, with 2.4% of children reported to be suffering from all 3 conditions. The main risk factors for wheeze were male sex and smoking in the home. Ninety-five (28.7%) children with wheeze had no diagnosis of asthma. Of these children 36 (37.9%) had wheeze with exercise, 55 (59.9%) had a dry cough not associated with a cold or flu and 13 (13.7%) reported more than 4 attacks of wheeze in the last year. This study suggests underdiagnosis and undertreatment of atopic illness in this age group. It also suggests decreasing exposure to passive smoking and early recognition and treatment will improve quality of life for many children.
Descriptors
Child, Eczema/epidemiology, Female, Humans, Ireland/epidemiology, Male, Prevalence, Respiratory Sounds, Rhinitis/epidemiology, Risk Factors, Sex Factors, Surveys and Questionnaires, Tobacco Smoke Pollution/adverse effects
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Data Source
Authors
Harty,S. B., Sheridan,A., Howell,F., Nicholson,A.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Central nervous system involvement in patients with decompression illness 2003 Department of Neurosurgery, Division of Hyperbaric Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
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Print(0)
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Journal Article
Periodical, Full
Sangyo eiseigaku zasshi = Journal of occupational health
Periodical, Abbrev.
Sangyo Eiseigaku Zasshi
Pub Date Free Form
May
Volume
45
Issue
3
Start Page
97
Other Pages
104
Notes
LR: 20061115; JID: 9507473; RF: 54; ppublish
Place of Publication
Japan
ISSN/ISBN
1341-0725; 1341-0725
Accession Number
PMID: 12833851
Language
jpn
SubFile
English Abstract; Journal Article; Review; IM
DOI
Output Language
Unknown(0)
PMID
12833851
Abstract
Dysbarism or decompression illness (DCI), a general term applied to all pathological changes secondary to altered environmental pressure, has two forms decompression sickness (DCS) and arterial gas embolism (AGE) after pulmonary barotrauma. Cerebral and spinal disorders have been symptomatically categorized as AGE and DCS, respectively. Magnetic resonance images (MRIs) of divers with DCI showed multiple cerebral infarction in the terminal and border zones of the brain arteries. In addition, there were no differences between MRI findings for compressed air and breath-hold divers. Although the pathogenesis of the brain is not well understood, we propose that arterialized bubbles passing through the lungs and heart involved the brain. From the mechanisms of bubble formation, however, this disorder has been classified as DCS. We propose that there is a difference between clinical and mechanical diagnoses in the criteria of brain DCI. In contrast to brain injury, the spinal cord is involved only in compressed air divers, and is caused by disturbed venous circulation due to bubbles in the epidural space. The best approach to prevent diving accidents is to make known the problems for professional and amateur divers.
Descriptors
Central Nervous System/pathology, Decompression Sickness/diagnosis/etiology/pathology/therapy, Diving/adverse effects, Embolism, Air/diagnosis/etiology/pathology/therapy, Humans, Hyperbaric Oxygenation, Magnetic Resonance Imaging, Occupational Exposure/adverse effects
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Database
Publisher
Data Source
Authors
Kohshi,K., Katoh,T., Abe,H., Wong,R. M.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Cigarette smoking, snuff use and alcohol drinking: coexisting risk behaviours for oral health in young males 2003 Centre for Tobacco Prevention, Stockholm Centre of Public Health, Stockholm County Council, Sweden. seppo.wickholm@smd.sll.se
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Print(0)
Ref Type
Journal Article
Periodical, Full
Community dentistry and oral epidemiology
Periodical, Abbrev.
Community Dent.Oral Epidemiol.
Pub Date Free Form
Aug
Volume
31
Issue
4
Start Page
269
Other Pages
274
Notes
LR: 20090129; JID: 0410263; ppublish
Place of Publication
Denmark
ISSN/ISBN
0301-5661; 0301-5661
Accession Number
PMID: 12846849
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; D; IM
DOI
46 [pii]
Output Language
Unknown(0)
PMID
12846849
Abstract
OBJECTIVES: The use of oral moist snuff (snus) is widespread among Swedish men, but little is known about the use in adolescents. The aim of this study was to describe patterns of snuff dipping, smoking and alcohol drinking in a sample (n = 6287) of 9th grade male students participating in a census survey in the Stockholm region. RESULTS: About 20% of the sample reported use of snus, and more than two-thirds of snus users were also cigarette smokers. Among current nonusers of tobacco (66% of the sample), 14% reported frequent binge drinking, in contrast to 49% among current exclusive cigarette smokers, 60% among exclusive snus users and 69% among users of both cigarettes and snus. The estimated mean annual consumption of alcohol was 5-10 times higher among tobacco users than among nonusers, with users of snus consuming more alcohol than smokers. Compared to non- or minimal drinkers, heavy alcohol drinkers had a disproportionately higher risk to report snus use, after adjustment for smoking behaviour (OR = 16.7, 95% CI 12.9-21.7). When the analysis was restricted to users of only one type of tobacco, heavy drinkers were twice as likely to report snus, rather than cigarette, use. CONCLUSIONS: Both tobacco use and alcohol drinking have been independently associated with a variety of pathological oral conditions in adults. These behaviours coexist in early adolescence. Their effect on oral health need to be investigated in prospective studies and should be of concern to the dental professionals.
Descriptors
Adolescent, Adolescent Behavior, Alcohol Drinking/epidemiology, Analysis of Variance, Codependency (Psychology), Humans, Male, Odds Ratio, Regression Analysis, Smoking/epidemiology, Sweden/epidemiology, Tobacco Use Disorder/epidemiology, Tobacco, Smokeless
Links
Book Title
Database
Publisher
Data Source
Authors
Wickholm,S., Galanti,M. R., Soder,B., Gilljam,H.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Tensile bond strengths of five luting agents to two CAD-CAM restorative materials and enamel 2003 Department of Dental Public Health and Dental Hygiene, Houston Dental Branch, University of Texas, Houston, Texas, USA. Jeffrey.C.Chang@uth.tmc.edu
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Print(0)
Ref Type
Journal Article
Periodical, Full
The Journal of prosthetic dentistry
Periodical, Abbrev.
J.Prosthet.Dent.
Pub Date Free Form
Jul
Volume
90
Issue
1
Start Page
18
Other Pages
23
Notes
LR: 20151119; JID: 0376364; 0 (Boron Compounds); 0 (Composite Resins); 0 (Dental Cements); 0 (Dicor ceramic); 0 (EnForce-Sure Cure); 0 (FujiPlus); 0 (Glass Ionomer Cements); 0 (Methacrylates); 0 (Methylmethacrylates); 0 (Panavia TPN-S); 0 (Phosphates); 0
Place of Publication
United States
ISSN/ISBN
0022-3913; 0022-3913
Accession Number
PMID: 12869970
Language
eng
SubFile
Journal Article; D; IM
DOI
10.1016/S0022391303002178 [doi]
Output Language
Unknown(0)
PMID
12869970
Abstract
STATEMENT OF PROBLEM: CAD-CAM restorative materials are popular because they have high esthetic value and short fabrication time. Unfortunately these materials are brittle and tend to fracture under heavy occlusal load. PURPOSE: The purpose of this in vitro study was to investigate the tensile bond strengths of 5 luting agents to 2 CAD-CAM restorative materials and enamel. Material and methods One hundred truncated cones, with a 3-mm diameter bonding surface and 5-mm diameter base, were fabricated with Dicor MGC (n=50) or Cerec Vitablocs Mark II (n=50) with the Cerec system. Enamel surfaces of the crowns of 100 freshly extracted noncarious, anterior teeth, stored in 0.9% saline solution with 0.25% sodium azide (NaN(3)) as disinfectant, were prepared with sandpaper disks (320-grit size) on the Buehler Variable Speed Grinder-Polisher. The bonding surfaces of the Dicor MGC or Cerec Vitablocs Mark II truncated cones from each of the 2 tests systems were airborne particle abraded, chemically etched, and cemented on the prepared enamel surface of the tooth specimens with 5 luting agents: Vita Cerec Duo Cement, EnForce, Panavia 21, C&B Metabond, and Fuji Duet. All specimens were thermocycled 1000 times, in 5 degrees C and 55 degrees C water with a dwell time of 30 seconds in each temperature. Tensile force (MPa) was applied perpendicular to the bonding surfaces of the specimens with a universal testing machine until fracture. Fracture patterns were examined with a microscope at original magnification x10. The differences in the tensile bond strengths were analyzed with the Tukey-Kramer test (alpha=.05). RESULTS: The mean tensile bond strengths of Dicor MGC bonding to enamel with luting agents in descending order in MPa were EnForce (10.12 +/- 2.25), Panavia 21 (7.28 +/- 1.77), Cerec Duo (5.32 +/- 1.65), Fuji Duet (3.73 +/- 1.78), and C&B Metabond (3.11 +/- 1.11). EnForce had a significantly higher bond strength than C&B Metabond, Fuji Duet, Cerec Duo, and Panavia 21 (P=.05). The mean tensile bond strengths of Cerec Vitablocs Mark II bonding to enamel with luting agents in descending order in MPa were as follow: Fuji Duet (11.51 +/- 2.79), EnForce (9.44 +/- 2.03), C&B Metabond (8.98 +/- 3.29), Cerec Duo (8.66 +/- 4.71), and Panavia 21 (5.10 +/- 2.49). Within this group, there was no significant difference between Fuji Duet and EnForce, but the bond strength of Fuji Duet was significantly higher than that of C&B Metabond, Panavia 21, and Cerec Duo (P=.05). The mean tensile strength of all the luting agents bonded to Cerec Vitablocs Mark II and enamel (8.74 MPa) was higher than Dicor MGC and enamel (5.91 MPa). CONCLUSION: In this in vitro study; within the Dicor MGC group, the mean bond strength of Panavia 21 with enamel was significantly higher than C&B Metabond, Panavia 21, Cerec Duo, and Fuji Duet. Within the Cerec Vitablocs Mark II group, the mean bond strength of Fuji Duet was significantly higher than C&B Metabond, Panavia 21, and Cerec Duo but was not significantly higher than EnForce.
Descriptors
Acid Etching, Dental, Bisphenol A-Glycidyl Methacrylate/chemistry, Boron Compounds/chemistry, Cementation, Ceramics/chemistry, Composite Resins/chemistry, Computer-Aided Design, Dental Bonding, Dental Cements/chemistry, Dental Enamel/ultrastructure, Dental Porcelain/chemistry, Dental Restoration, Permanent, Glass Ionomer Cements, Humans, Materials Testing, Methacrylates/chemistry, Methylmethacrylates/chemistry, Phosphates/chemistry, Resin Cements/chemistry, Stress, Mechanical, Surface Properties, Tensile Strength
Links
Book Title
Database
Publisher
Data Source
Authors
Chang,J. C., Hart,D. A., Estey,A. W., Chan,J. T.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Bond strengths of porcelain laminate veneers to tooth surfaces prepared with acid and Er,Cr:YSGG laser etching 2003 Department of Prosthodontics, Selcuk University, Konya, Turkey. asli_u@hotmail.com
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
The Journal of prosthetic dentistry
Periodical, Abbrev.
J.Prosthet.Dent.
Pub Date Free Form
Jul
Volume
90
Issue
1
Start Page
24
Other Pages
30
Notes
LR: 20121115; JID: 0376364; 0 (Aluminum Silicates); 0 (IPS-Empress ceramic); 0 (Maleates); 0 (Phosphoric Acids); 0 (Resin Cements); 12001-21-7 (Dental Porcelain); 179240-22-3 (Variolink); 91XW058U2C (maleic acid); ppublish
Place of Publication
United States
ISSN/ISBN
0022-3913; 0022-3913
Accession Number
PMID: 12869971
Language
eng
SubFile
Journal Article; D; IM
DOI
10.1016/S002239130300235X [doi]
Output Language
Unknown(0)
PMID
12869971
Abstract
STATEMENT OF PROBLEM: The erbium, chromium: yttrium, scandium, gallium, garnet (Er,Cr:YSGG) hydrokinetic laser system has been successful in the ablation of dental tissues. It has been reported that this system is also useful for preparing tooth surfaces for adhesion, but results to date have been controversial. PURPOSE: This in vitro study evaluated the bond strengths of porcelain laminate veneers to tooth surfaces after etching with acid and Er,Cr:YSGG laser conditioning. Material and method Forty extracted caries- and restoration-free human maxillary central incisors were used. The teeth were sectioned 2 mm below the cementoenamel junction. The crowns were embedded in autopolymerizing acrylic resin with the labial surfaces facing up. The labial surfaces were prepared with.05 mm reduction to receive porcelain veneers. The teeth were divided into 4 groups of 10 specimens. Thirty specimens received 1 of the following surface treatments before the bonding of IPS Empress 2 laminate veneers: (1) laser radiation from an Er,Cr:YSGG laser unit; (2) 37% orthophosphoric acid; and (3) 10% maleic acid. Ten specimens received no surface treatment and served as the control group. The veneers were bonded with dual-polymerizing resin, Variolink II. One microtensile specimen from each of the cervical and incisal thirds measuring 1.2 x 1.2 mm was prepared with a slow-speed diamond saw sectioning machine with a diamond-rim blade. These specimens were attached to opposing arms of the microtensile testing device with cyanoacrylate adhesive and fractured under tension at a crosshead speed of 1 mm/min, and the maximum load at fracture (Kg) was recorded. The data were analyzed with a 2-way analysis of variance and Tukey HSD tests (alpha=.05). RESULTS: No statistically significant differences were found among the bond strengths of veneers bonded to tooth surfaces etched with Er,Cr:YSGG laser (12.1 +/- 4.4 MPa), 37% orthophosphoric acid (13 +/- 6.5 MPa), and 10% maleic acid (10.6 +/- 5.6 MPa). The control group demonstrated the lowest bond strength values in all test groups. Statistically significant differences were found between the bond strengths of cervical and incisal sections (P<.001). CONCLUSION: In vitro microtensile bond strengths of porcelain laminate veneers bonded to tooth surfaces that were laser-etched showed results similar to orthophosphoric acid or maleic acid etched tooth surfaces.
Descriptors
Acid Etching, Dental, Aluminum Silicates/chemistry, Analysis of Variance, Dental Bonding, Dental Enamel/ultrastructure, Dental Porcelain/chemistry, Dental Veneers, Humans, Lasers, Maleates/chemistry, Materials Testing, Microscopy, Electron, Scanning, Phosphoric Acids/chemistry, Resin Cements/chemistry, Stress, Mechanical, Surface Properties, Tensile Strength, Tooth Preparation/methods
Links
Book Title
Database
Publisher
Data Source
Authors
Usumez,A., Aykent,F.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Most smokeless tobacco use is not a causal gateway to cigarettes: using order of product use to evaluate causation in a national US sample 2003 Department of Biobehavioral Health, Pennsylvania State University, PA, USA. ltk1@psu.edu
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Addiction (Abingdon, England)
Periodical, Abbrev.
Addiction
Pub Date Free Form
Aug
Volume
98
Issue
8
Start Page
1077
Other Pages
1085
Notes
LR: 20041117; JID: 9304118; CIN: Addiction. 2004 Feb;99(2):260-2; author reply 262-4. PMID: 14756720; ppublish
Place of Publication
England
ISSN/ISBN
0965-2140; 0965-2140
Accession Number
PMID: 12873242
Language
eng
SubFile
Journal Article; IM
DOI
460 [pii]
Output Language
Unknown(0)
PMID
12873242
Abstract
AIMS: To evaluate non-causal and causal patterns of smokeless tobacco (SLT) and cigarette use; to assess the prevalence of 'non-gateway' and possible 'gateway' patterns of SLT use. DESIGN AND SETTING: Data from the Cancer Control Supplement to the 1987 National Health Interview Survey, a representative survey of non-institutionalized adults in the United States. From reported age at first use, participants were categorized by type and sequence of tobacco product use. SUDAAN 8.0.1 was used for statistical analyses. PARTICIPANTS: Males aged 18-34 (n = 3454), weighted to provide estimates of the US population. A subsample of males aged 23-34 (n = 2614) was analyzed to minimize the possibility of future product switching. MEASUREMENTS: Smoking status, smokeless tobacco (snuff, chewing tobacco, both) use status, age at regular use of cigarettes, age at first use of smokeless tobacco. FINDINGS: Of those 23-34-year-olds who had ever used SLT with or without cigarettes, 77.2% (95% CI: 71.3, 83.3) were classifiable as non-gateway users in that 35.0% (95% CI: 29.9, 40.1) had only used SLT and 42.2% (95% CI: 36.8, 47.7) had used cigarettes first. Cigarette use in younger cohorts was less common, despite increased SLT use. Those who used cigarettes before moist snuff were 2.1 times more likely to have quit smoking (95% CI 1.21,6.39) than cigarette-only users. CONCLUSIONS: The large majority of SLT users are non-gateway users. Causal gateway effects should be of minor concern for policy. SLT may be more likely to prevent smoking than cause it.
Descriptors
Adolescent, Adult, Cohort Studies, Humans, Male, Smoking/psychology, Smoking Cessation/psychology, Sweden, Tobacco Use Disorder/etiology, Tobacco, Smokeless, United States
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Book Title
Database
Publisher
Data Source
Authors
Kozlowski,L. T., O'Connor,R. J., Edwards,B. Q., Flaherty,B. P.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Embolic inner ear decompression illness: correlation with a right-to-left shunt 2003 Department of Otolarynology-Head and Neck Surgery, University of Heidelberg, Germany. christpoh_klingmann@med.uni-heidekberg.de
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
The Laryngoscope
Periodical, Abbrev.
Laryngoscope
Pub Date Free Form
Aug
Volume
113
Issue
8
Start Page
1356
Other Pages
1361
Notes
LR: 20041117; JID: 8607378; CIN: Laryngoscope. 2004 Aug;114(8):1510; author reply 1510-1. PMID: 15280736; ppublish
Place of Publication
United States
ISSN/ISBN
0023-852X; 0023-852X
Accession Number
PMID: 12897559
Language
eng
SubFile
Journal Article; IM
DOI
10.1097/00005537-200308000-00017 [doi]
Output Language
Unknown(0)
PMID
12897559
Abstract
OBJECTIVES/HYPOTHESIS: Inner ear decompression illness is thought to be a rare phenomenon in recreational divers, isolated signs and symptoms of inner ear dysfunction usually being attributed to inner ear barotrauma. STUDY DESIGN: We present 11 cases of inner ear dysfunction in nine divers with inner ear decompression illness. RESULTS: All nine divers had significant right-to-left shunt as diagnosed by transcranial Doppler sonography. CONCLUSIONS: The authors thought that mechanism of causation in these cases may have been intravascular bubble emboli and that inner ear decompression illness may be more common among recreational divers than currently recognized. Failure to treat inner ear decompression illness with recompression therapy can result in permanent disability. Because the differential diagnosis between inner ear barotrauma and inner ear decompression illness can be impossible, the authors suggested that divers who present with inner ear symptoms following a dive should have recompression immediately after having undergone bilateral paracentesis.
Descriptors
Decompression Sickness/diagnosis/physiopathology/therapy, Diving/injuries, Embolism, Air/etiology/physiopathology/therapy, Heart Septal Defects, Atrial/complications/diagnosis/physiopathology, Humans, Hyperbaric Oxygenation, Labyrinth Diseases/diagnosis/etiology/physiopathology/therapy
Links
Book Title
Database
Publisher
Data Source
Authors
Klingmann,C., Benton,P. J., Ringleb,P. A., Knauth,M.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Microbial and physiological characterization of weakly amylolytic but fast-growing lactic acid bacteria: a functional role in supporting microbial diversity in pozol, a Mexican fermented maize beverage 2003 Departamento de Alimentos y Biotecnologia, Facultad de Quimica, UNAM, 04510 Mexico D.F., Mexico.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Applied and Environmental Microbiology
Periodical, Abbrev.
Appl.Environ.Microbiol.
Pub Date Free Form
Aug
Volume
69
Issue
8
Start Page
4367
Other Pages
4374
Notes
LR: 20140611; JID: 7605801; 0 (RNA, Ribosomal, 16S); 9005-25-8 (Starch); OID: NLM: PMC169132; ppublish
Place of Publication
United States
ISSN/ISBN
0099-2240; 0099-2240
Accession Number
PMID: 12902217
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
Output Language
Unknown(0)
PMID
12902217
Abstract
Pozol is an acid beverage obtained from the natural fermentation of nixtamal (heat- and alkali-treated maize) dough. The concentration of mono- and disaccharides from maize is reduced during nixtamalization, so that starch is the main carbohydrate available for lactic acid fermentation. In order to provide some basis to understand the role of amylolytic lactic acid bacteria (ALAB) in this fermented food, their diversity and physiological characteristics were determined. Forty amylolytic strains were characterized by phenotypic and molecular taxonomic methods. Four different biotypes were distinguished via ribotyping; Streptococcus bovis strains were found to be predominant. Streptococcus macedonicus, Lactococcus lactis, and Enterococcus sulfureus strains were also identified. S. bovis strain 25124 showed extremely low amylase yield relative to biomass (139 U g [cell dry weight](-1)) and specific rate of amylase production (130.7 U g [cell dry weight](-1) h(-1)). In contrast, it showed a high specific growth rate (0.94 h(-1)) and an efficient energy conversion yield to bacterial cell biomass (0.31 g of biomass g of substrate(-1)). These would confer on the strain a competitive advantage and are the possible reasons for its dominance. Transient accumulation of maltooligosaccharides during fermentation could presumably serve as energy sources for nonamylolytic species in pozol fermentation. This would explain the observed diversity and the dominance of nonamylolytic lactic acid bacteria at the end of fermentation. These results are the first step to understanding the importance of ALAB during pozol fermentation.
Descriptors
Beverages, Colony Count, Microbial, Fermentation, Lactobacillus/metabolism, Lactococcus lactis/metabolism, RNA, Ribosomal, 16S/genetics, Ribotyping, Starch/metabolism, Streptococcus/metabolism, Zea mays/microbiology
Links
Book Title
Database
Publisher
Data Source
Authors
Diaz-Ruiz,G., Guyot,J. P., Ruiz-Teran,F., Morlon-Guyot,J., Wacher,C.
Original/Translated Title
URL
Date of Electronic
PMCID
PMC169132
Editors
Architecture, influencing factors, and sensitivity to antifungal agents of Candida biofilm 2002 Department of Dermatology, PUMC Hospital, CAMS, PUMC, Beijing 100730, China. sunqnzhy@yahoo.com
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Zhongguo yi xue ke xue yuan xue bao.Acta Academiae Medicinae Sinicae
Periodical, Abbrev.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
Pub Date Free Form
Aug
Volume
24
Issue
4
Start Page
385
Other Pages
388
Notes
LR: 20131121; JID: 8006230; 0 (Antifungal Agents); 304NUG5GF4 (Itraconazole); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); ppublish
Place of Publication
China
ISSN/ISBN
1000-503X; 1000-503X
Accession Number
PMID: 12905659
Language
chi
SubFile
English Abstract; Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
12905659
Abstract
OBJECTIVE: To establish a model of Candida biofilm and to explore its characteristics, ultrastructure, influences by saliva and serum, and sensitivity to antifungal agents. METHODS: Evaluations of the in vitro growth kinetics, influences by saliva and serum, and sensitivity to antifungal agents of Candida biofilm were performed with the abated tetrazolium salt XTT method on a 96-well microtire petri dish. The ultrastructure of Candida biofilm was observed under Confocal Laser Scanning Microscope (CLSM). RESULTS: The bioactivity of Candida biofilm increased with culturing time and serum could obviously increase the action of biofilm. The Candida biofilm was significantly resistant to routine antifungal agents. CONCLUSION: The Candida cells adhered in biofilms are significantly different in morphology from those in suspension and are resistant to routine antifungal agents such as Amphotericine B, Fluconazole and Itraconazole.
Descriptors
Amphotericin B/pharmacology, Antifungal Agents/pharmacology, Biofilms/drug effects, Candida/drug effects/ultrastructure, Drug Resistance, Fungal, Fluconazole/pharmacology, Itraconazole/pharmacology, Microbial Sensitivity Tests, Microscopy, Confocal
Links
Book Title
Database
Publisher
Data Source
Authors
Sun,Q. N., Fang,K.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Formation of natural biofilms during chlorine dioxide and u.v. disinfection in a public drinking water distribution system 2003 Department of Environmental Microbiology, Water Technology and Geotechnology Division, Institute for Technical Chemistry, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany. thomas.schwartz@itc-wgt.fzk.de
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of applied microbiology
Periodical, Abbrev.
J.Appl.Microbiol.
Pub Date Free Form
Volume
95
Issue
3
Start Page
591
Other Pages
601
Notes
LR: 20121115; JID: 9706280; 0 (Chlorine Compounds); 0 (Disinfectants); 0 (Oxides); 8061YMS4RM (chlorine dioxide); ppublish
Place of Publication
England
ISSN/ISBN
1364-5072; 1364-5072
Accession Number
PMID: 12911708
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
2019 [pii]
Output Language
Unknown(0)
PMID
12911708
Abstract
AIMS: The influence of two disinfection techniques on natural biofilm development during drinking water treatment and subsequent distribution is compared with regard to the supply of a high-quality drinking water. METHODS AND RESULTS: The growth of biofilms was studied using the biofilm device technique in a real public technical drinking water asset. Different pipe materials which are commonly used in drinking water facilities (hardened polyethylene, polyvinyl chloride, steel and copper) were used as substrates for biofilm formation. Apart from young biofilms, several months old biofilms were compared in terms of material dependence, biomass and physiological state. Vital staining of biofilms with 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) and the DNA-specific 4',6-diamidino-2-phenylindole (DAPI) staining resulted in a significant difference in physiological behaviour of biofilm populations depending on the disinfection technique. Compared with chlorine dioxide disinfection (0.12-0.16 mg l-1), the respiratory activities of the micro-organisms were increased on all materials during u.v. disinfection (u.v.254; 400 J m-2). The biofilm biocoenosis was analysed by in situ hybridization with labelled oligonucleotides specific for some subclasses of Proteobacteria. Using PCR and additional hybridization techniques, the biofilms were also tested for the presence of Legionella spp., atypical mycobacteria and enterococci. The results of the molecular-biological experiments in combination with cultivation tests showed that enterococci were able to pass the u.v. disinfection barrier and persist in biofilms of the distribution system, but not after chlorine dioxide disinfection. CONCLUSIONS: The results indicated that bacteria are able to regenerate and proliferate more effectively after u.v. irradiation at the waterworks, and chlorine dioxide disinfection appears to be more applicative to maintain a biological stable drinking water. SIGNIFICANCE AND IMPACT OF THE STUDY: As far as the application of u.v. disinfection is used for conditioning of critical water sources for drinking water, the efficiency of u.v. irradiation in natural systems should reach a high standard to avoid adverse impacts on human health.
Descriptors
Biofilms/drug effects/growth & development/radiation effects, Chlorine Compounds/pharmacology, Colony Count, Microbial, Disinfectants/pharmacology, Disinfection/methods, Enterococcus/drug effects/growth & development/radiation effects, Humans, Oxides/pharmacology, Water Microbiology, Water Purification/methods, Water Supply
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Schwartz,T., Hoffmann,S., Obst,U.
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