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Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals 2011 Departamento de Analises Clinicas, Faculdade de Ciencias Farmaceuticas, Universidade Estadual Paulista, Araraquara, SP, Brasil. rehepi@gmail.com
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Print(0)
Ref Type
Journal Article
Periodical, Full
Memorias do Instituto Oswaldo Cruz
Periodical, Abbrev.
Mem.Inst.Oswaldo Cruz
Pub Date Free Form
Sep
Volume
106
Issue
6
Start Page
646
Other Pages
654
Notes
LR: 20131121; JID: 7502619; 0 (Antifungal Agents); 0 (Hemodialysis Solutions); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); 2011/01/09 [received]; 2011/08/16 [accepted]; ppublish
Place of Publication
Brazil
ISSN/ISBN
1678-8060; 0074-0276
Accession Number
PMID: 22012217
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
S0074-02762011000600002 [pii]
Output Language
Unknown(0)
PMID
22012217
Abstract
Candida parapsilosis, currently divided into three distinct species, proliferates in glucose-rich solutions and has been associated with infections resulting from the use of medical devices made of plastic, an environment common in dialysis centres. The aims of this study were (i) to screen for Candida orthopsilosis and Candida metapsilosis (100 environmental isolates previously identified as C. parapsilosis), (ii) to test the ability of these isolates to form biofilm and (iii) to investigate the in vitro susceptibility of Candida spp biofilms to the antifungal agents, fluconazole (FLC) and amphotericin B (AMB). Isolates were obtained from a hydraulic circuit collected from a haemodialysis unit. Based on molecular criteria, 47 strains were re-identified as C. orthopsilosis and 53 as C. parapsilosis. Analyses using a formazan salt reduction assay and total viable count, together with microscopy studies, revealed that 72 strains were able to form biofilm that was structurally similar, but with minor differences in morphology. A microtitre-based colorimetric assay used to test the susceptibility of fungal biofilms to AMB and FLC demonstrated that the C. parapsilosis complex displayed an increased resistance to these antifungal agents. The results from these analyses may provide a basis for implementing quality controls and monitoring to ensure the microbiological purity of dialysis water, including the presence of yeast.
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Book Title
Database
Publisher
Data Source
Authors
Pires,R.H., Santos,J.M., Zaia,J.E., Martins,C.H., Mendes-Giannini,M.J.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
The Paraty artisanal fishery (southeastern Brazilian coast): ethnoecology and management of a social-ecological system (SES) 2012 UNICAMP: CAPESCA, LEPAC (Paraty) and CMU, CP 6023 Campinas, Brazil. alpinab@uol.com.br
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of ethnobiology and ethnomedicine
Periodical, Abbrev.
J.Ethnobiol Ethnomed
Pub Date Free Form
27-Jun
Volume
8
Issue
Start Page
22
Other Pages
8/22/4269
Notes
LR: 20150224; JID: 101245794; OID: NLM: PMC3476967; 2012/02/16 [received]; 2012/05/28 [accepted]; 2012/06/27 [aheadofprint]; epublish
Place of Publication
England
ISSN/ISBN
1746-4269; 1746-4269
Accession Number
PMID: 22738073
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1186/1746-4269-8-22 [doi]
Output Language
Unknown(0)
PMID
22738073
Abstract
This study intends to give recommendations to the management of Paraty fishery in Brazil through an interplay of local and scientific knowledge. In particular, the objectives are the following: 1) to describe the Paraty fishery; 2) to compare the fishermen's local ecological knowledge with recorded fish landings and previous studies in Paraty; 3) to combine the data on local fishing and on local/Caicara livelihoods with the SES (social-ecological systems) Model. The methods include a systematic survey of fishing in Tarituba and Praia Grande, which are located in the northern end and the central part of the Paraty municipality, respectively. For four days each month, systematic data on catches at landing points were collected, as well as macroscopic gonad analysis data for the fishes Centropomus parallelus and C. undecimalis (snook, robalo), Epinephelus marginatus (grouper, garoupa), Scomberomorus cavalla (King mackerel, cavala), and Lutjanus synagris (Lane snapper, vermelho). Spring and summer are important seasons during which some species reproduce, and the integration of fishing periods for some target species could assist in fishing management through the use of closed seasons. Fishermen could obtain complementary earnings from tourism and from the "defeso system" (closed season including a salary payment) to conserve fishing stocks. The SES model facilitates an understanding of the historical context of fishing, its economic importance for local livelihoods, the constraints from conservation measures that affect fishermen, and the management processes that already exist, such as the defeso. If used to integrate fishing with complementary activities (tourism), such a system could improve the responsibility of fishermen regarding the conservation of fish stocks.
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Book Title
Database
Publisher
Data Source
Authors
Begossi,A., Salyvonchyk,S., Nora,V., Lopes,P.F., Silvano,R.A.
Original/Translated Title
URL
Date of Electronic
20120627
PMCID
PMC3476967
Editors
Ethyl 2-[4-(morpholin-4-yl)phen-yl]-1-[3-(2-oxopyrrolidin-1-yl)prop-yl]-1H-1,3-benzimid azole-5-carboxyl-ate monohydrate 2012 Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia.
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Print(0)
Ref Type
Journal Article
Periodical, Full
Acta crystallographica.Section E, Structure reports online
Periodical, Abbrev.
Acta Crystallogr.Sect.E.Struct.Rep.Online
Pub Date Free Form
1-Oct
Volume
68
Issue
Pt 10
Start Page
o2967
Other Pages
8
Notes
LR: 20130304; JID: 101089178; OID: NLM: PMC3470336; 2012/08/29 [received]; 2012/09/14 [accepted]; 2012/09/22 [epublish]; ppublish
Place of Publication
United States
ISSN/ISBN
1600-5368; 1600-5368
Accession Number
PMID: 23125749
Language
eng
SubFile
Journal Article
DOI
10.1107/S1600536812039268 [doi]
Output Language
Unknown(0)
PMID
23125749
Abstract
The asymmetric unit of the title compound, C(27)H(32)N(4)O(4).H(2)O, contains two independent benzimidazole-5-carboxyl-ate mol-ecules and two water mol-ecules. In both main mol-ecules, the pyrrolidine rings are in an envelope conformation with a methyl-ene C atom as the flap. The morpholine rings adopt chair conformations. Both benzimidazole rings are essentially planar, with maximum deviations of 0.008 (1) A, and form dihedral angles of 37.65 (6) and 45.44 (6) degrees with the benzene rings. In one mol-ecule, an intra-molecular C-Hcdots, three dots, centeredO hydrogen bond forms an S(7) ring motif. In the crystal, O-Hcdots, three dots, centeredO and O-Hcdots, three dots, centeredN hydrogen bonds connect pairs of main mol-ecules and pairs of water mol-ecules into two independent centrosymmetric four-compoment aggregates. These aggregates are connect by C-Hcdots, three dots, centeredO hydrogen bonds leading to the formation of a three-dimensional network, which is stabilized by C-Hcdots, three dots, centeredpi interactions.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Yoon,Y.K., Ali,M.A., Choon,T.S., Arshad,S., Razak,I.A.
Original/Translated Title
URL
Date of Electronic
20120922
PMCID
PMC3470336
Editors
Effect of disinfectant, water age, and pipe material on occurrence and persistence of Legionella, mycobacteria, Pseudomonas aeruginosa, and two amoebas 2012 Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, Virginia 24061, USA.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Environmental science & technology
Periodical, Abbrev.
Environ.Sci.Technol.
Pub Date Free Form
6-Nov
Volume
46
Issue
21
Start Page
11566
Other Pages
11574
Notes
LR: 20131121; JID: 0213155; 0 (Chloramines); 0 (Disinfectants); 0 (Drinking Water); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); 0 (Water Pollutants); 10599-90-3 (chloramine); 4R7X1O2820 (Chlorine); 9002-86-2 (Polyvinyl Chloride); E1UOL152H7 (Iron); 2012/
Place of Publication
United States
ISSN/ISBN
1520-5851; 0013-936X
Accession Number
PMID: 23046164
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; IM
DOI
10.1021/es303212a [doi]
Output Language
Unknown(0)
PMID
23046164
Abstract
Opportunistic pathogens represent a unique challenge because they establish and grow within drinking water systems, yet the factors stimulating their proliferation are largely unknown. The purpose of this study was to examine the influence of pipe materials, disinfectant type, and water age on occurrence and persistence of three opportunistic pathogens (Legionella pneumophila, Mycobacterium avium, and Pseudomonas aeruginosa), broader genera (Legionella and mycobacteria), and two amoeba hosts (Acanthamoeba spp. and Hartmanella vermiformis). Triplicate simulated distribution systems (SDSs) compared iron, cement, and PVC pipe materials fed either chlorinated or chloraminated tap water and were sampled at water ages ranging from 1 day to 5.7 days. Quantitative polymerase chain reaction quantified gene copies of target microorganisms in both biofilm and bulk water. Legionella, mycobacteria, P. aeruginosa, and both amoebas naturally colonized the six SDSs, but L. pneumophila and M. avium were not detected. Disinfectant type and dose was observed to have the strongest influence on the microbiota. Disinfectant decay was noted with water age, particularly in chloraminated SDSs (due to nitrification), generally resulting in increased microbial detection frequencies and densities with water age. The influence of pipe material became apparent at water ages corresponding to low disinfectant residual. Each target microbe appeared to display a distinct response to disinfectant type, pipe materials, water age, and their interactions. Differences between the first and the second samplings (e.g., appearance of Legionella, reduction in P. aeruginosa and Acanthamoeba) suggest a temporally dynamic drinking water microbial community.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Wang,H., Masters,S., Hong,Y., Stallings,J., Falkinham,J.O.,3rd, Edwards,M.A., Pruden,A.
Original/Translated Title
URL
Date of Electronic
20121025
PMCID
Editors
Molecular and phenotypic characterisation of extended spectrum beta-lactamase CTX-M Escherichia coli from farm animals in Great Britain 2012 Animal Health and Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, United Kingdom.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Research in veterinary science
Periodical, Abbrev.
Res.Vet.Sci.
Pub Date Free Form
Dec
Volume
93
Issue
3
Start Page
1142
Other Pages
1150
Notes
CI: Crown Copyright (c) 2012; JID: 0401300; 0 (Anti-Bacterial Agents); EC 3.5.2.6 (beta-Lactamases); 2011/12/07 [received]; 2012/04/30 [revised]; 2012/05/07 [accepted]; 2012/06/15 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
1532-2661; 0034-5288
Accession Number
PMID: 22704719
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.rvsc.2012.05.001 [doi]
Output Language
Unknown(0)
PMID
22704719
Abstract
The aim of this study was to characterise CTX-M Escherichia coli isolates from cattle, chickens and turkeys in Great Britain with respect to CTX-M sequence type, replicon type, ability to transfer plasmids, and for the presence of antibiotic resistance, fitness and virulence genes as determined by micro-arrays. The main CTX-M enzymes identified in E. coli from cattle, chicken and turkeys were 14 and 15, 1 and 15, and 1 and 14 respectively. Most isolates from different animal species transferred their plasmids with similar frequencies. The plasmid replicon type I1-lambda was most common and seen in 23%, 95% and 50% of the isolates tested from cattle, chickens and turkeys respectively, whilst types F, FIA, FIB and K were common to isolates from cattle and turkeys only. Thirty-eight different antibiotic resistance genes were detected by micro-array including aad genes, blaCTX-M, blaTEM, cat genes dfrA, floR, strA, strB, sul, sul2 tetA and tetB. Thirty-nine different fitness and virulence genes were also detected by-micro-array, including espP, ireA, lpfA, mchF, prfB and tsh. Fisher exact test and hierarchical clustering of the antibiotic resistance and virulence gene results showed some genes were more commonly associated with isolates from chickens or cattle. This study provides a baseline of the characteristics of CTX-M E. coli isolates from animals in Great Britain and suggests that chicken and cattle CTX-M E. coli represent different populations.
Descriptors
Links
Book Title
Database
Publisher
. Published by Elsevier India Pvt Ltd. All rights reserved
Data Source
Authors
Toszeghy,M., Phillips,N., Reeves,H., Wu,G., Teale,C., Coldham,N., Randall,L.
Original/Translated Title
URL
Date of Electronic
20120615
PMCID
Editors
A review of multicomponent interventions to prevent and control tobacco use among college students 2012 Department of Health Education, Teachers College, Columbia University, New York, NY, USA. Kirsten.rodgers@emory.edu
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of American college health : J of ACH
Periodical, Abbrev.
J.Am.Coll.Health
Pub Date Free Form
Volume
60
Issue
3
Start Page
257
Other Pages
261
Notes
JID: 8214119; ppublish
Place of Publication
United States
ISSN/ISBN
1940-3208; 0744-8481
Accession Number
PMID: 22420704
Language
eng
SubFile
Journal Article; Review; IM
DOI
10.1080/07448481.2011.587486 [doi]
Output Language
Unknown(0)
PMID
22420704
Abstract
OBJECTIVE: Multicomponent tobacco control programs have been implemented at the state and community levels and have led to a reduction in tobacco use. The purpose was to review the public health research literature on tobacco prevention and control programs on college campuses and derive evidence-based implications for comprehensive program implementation. METHODS: MEDLINE, PsycINFO, ERIC, and PubMed databases were used to search the research literature concerning tobacco prevention and control programs conducted on college campuses published between 2000 and 2009. RESULTS: No studies were found that implemented all 5 recommended components of a comprehensive program. Tobacco control programs containing policy and prevention education were used the most and promotion of tobacco-free environments and banning sales of tobacco products were used the least. CONCLUSION: The review suggests that despite the recommendation of comprehensive tobacco control programs to reduce tobacco use on college campuses, few institutions have implemented and evaluated programs consisting of multiple components.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Rodgers,K.C.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Occurrence and characteristics of extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae in food producing animals, minced meat and raw milk 2012 Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, Switzerland.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
BMC veterinary research
Periodical, Abbrev.
BMC Vet.Res.
Pub Date Free Form
7-Mar
Volume
8
Issue
Start Page
21
Other Pages
8/21/6148
Notes
LR: 20150225; JID: 101249759; EC 3.5.2.6 (beta-Lactamases); OID: NLM: PMC3319423; 2011/09/21 [received]; 2012/03/07 [accepted]; 2012/03/07 [aheadofprint]; epublish
Place of Publication
England
ISSN/ISBN
1746-6148; 1746-6148
Accession Number
PMID: 22397509
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1186/1746-6148-8-21 [doi]
Output Language
Unknown(0)
PMID
22397509
Abstract
BACKGROUND: The impact of food animals as a possible reservoir for extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae, and the dissemination of such strains into the food production chain need to be assessed. In this study 334 fecal samples from pigs, cattle, chicken and sheep were investigated at slaughter. Additionally, 100 raw milk samples, representing bulk tank milk of 100 different dairy farms, 104 minced meat (pork and beef) samples and 67 E. coli isolates from cattle E. coli mastitis were analyzed. RESULTS: As many as 15.3% of the porcine, 13.7% of the bovine, 8.6% of the sheep and 63.4% of the chicken fecal samples yielded ESBL producers after an enrichment step. In contrast, none of the minced meat, none of the bulk tank milk samples and only one of the mastitis milk samples contained ESBL producing strains. Of the total of 91 isolates, 89 were E. coli, one was Citrobacter youngae and one was Enterobacter cloacae. PCR analysis revealed that 78 isolates (85.7%) produced CTX-M group 1 ESBLs while six isolates (6.6%) produced CTX-M group 9 enzymes. Five detected ESBLs (5.5%) belonged to the SHV group and 2 isolates (2.2%) contained a TEM-type enzyme. A total of 27 CTX-M producers were additionally PCR-positive for TEM-beta-lactamase. The ESBL-encoding genes of 53 isolates were sequenced of which 34 produced CTX-M-1, 6 produced CTX-M-14, 5 produced CTX-M-15 and also 5 produced SHV-12. Two isolates produced TEM-52 and one isolate expressed a novel CTX-M group 1 ESBL, CTX-M-117. One isolate--aside from a CTX-M ESBL-- contained an additional novel TEM-type broad-spectrum beta-lactamase, TEM-186. CONCLUSIONS: The relatively high rates of ESBL producers in food animals and the high genetic diversity among these isolates are worrisome and indicate an established reservoir in farm animals.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Geser,N., Stephan,R., Hachler,H.
Original/Translated Title
URL
Date of Electronic
20120307
PMCID
PMC3319423
Editors
Antifungal susceptibility to amphotericin B, fluconazole, voriconazole, and flucytosine in Candida bloodstream isolates from 15 tertiary hospitals in Korea 2012 Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Annals of laboratory medicine
Periodical, Abbrev.
Ann.Lab.Med.
Pub Date Free Form
Nov
Volume
32
Issue
6
Start Page
426
Other Pages
428
Notes
LR: 20150222; JID: 101571172; 0 (Antifungal Agents); 0 (Pyrimidines); 0 (Triazoles); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); D83282DT06 (Flucytosine); JFU09I87TR (Voriconazole); OID: NLM: PMC3486937; OTO: NOTNLM; 2012/06/07 [received]; 2012
Place of Publication
Korea (South)
ISSN/ISBN
2234-3814; 2234-3806
Accession Number
PMID: 23130342
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.3343/alm.2012.32.6.426 [doi]
Output Language
Unknown(0)
PMID
23130342
Abstract
The in vitro antifungal susceptibility of 636 Candida bloodstream isolates collected from 15 tertiary hospitals in Korea was determined using the Vitek-2 yeast susceptibility system (bioMerieux, France). Overall susceptibility rates were 98.1%, 95.9%, 99.1%, and 97.3% for amphotericin B, fluconazole, voriconazole, and flucytosine, respectively. The results show that the rates of resistance to 4 antifungal drugs remain low among Candida bloodstream isolates in Korea.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Jung,S.I., Shin,J.H., Choi,H.J., Ju,M.Y., Kim,S.H., Lee,W.G., Park,Y.J., Lee,K., Korean Study Group for Candidemia
Original/Translated Title
URL
Date of Electronic
20121017
PMCID
PMC3486937
Editors
N-[4-(4-Bromo-phen-yl)thia-zol-2-yl]-4-(piperidin-1-yl)butanamide 2012
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Acta crystallographica.Section E, Structure reports online
Periodical, Abbrev.
Acta Crystallogr.Sect.E.Struct.Rep.Online
Pub Date Free Form
1-Jun
Volume
68
Issue
Pt 6
Start Page
o1665
Other Pages
Notes
JID: 101089178; OID: NLM: PMC3379262; 2012/04/28 [received]; 2012/04/29 [accepted]; 2012/05/12 [epublish]; ppublish
Place of Publication
United States
ISSN/ISBN
1600-5368; 1600-5368
Accession Number
PMID: 22719460
Language
eng
SubFile
Journal Article
DOI
10.1107/S1600536812019204 [doi]
Output Language
Unknown(0)
PMID
22719460
Abstract
In the title compound, C(18)H(22)BrN(3)OS, the piperidine ring adopts a chair conformation. The mean plane of the thia-zole ring forms dihedral angles of 23.97 (10) and 75.82 (10) degrees with the mean planes of its adjacent benzene and piperidine rings, respectively. An intra-molecular N-Hcdots, three dots, centeredN hydrogen bond generates an S(7) ring motif in the mol-ecule. In the crystal, no significant inter-moelcular hydrogen bonds are observed, but a weak pi-pi inter-action with a centroid-centroid distance of 3.8855 (13) A occurs.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Ghabbour,H.A., Kadi,A.A., El-Subbagh,H.I., Chia,T.S., Fun,H.K.
Original/Translated Title
URL
Date of Electronic
20120512
PMCID
PMC3379262
Editors
N-(4-Fluoro-benzo-yl)-N',N''-diisopropyl-phospho-ric triamide 2012 Department of Chemistry, Ferdowsi University of Mashhad, Mashhad, Iran.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Acta crystallographica.Section E, Structure reports online
Periodical, Abbrev.
Acta Crystallogr.Sect.E.Struct.Rep.Online
Pub Date Free Form
1-Dec
Volume
68
Issue
Pt 12
Start Page
o3406
Other Pages
7
Notes
LR: 20130418; JID: 101089178; OID: NLM: PMC3588995; 2012/11/06 [received]; 2012/11/09 [accepted]; 2012/11/24 [epublish]; ppublish
Place of Publication
United States
ISSN/ISBN
1600-5368; 1600-5368
Accession Number
PMID: 23476231
Language
eng
SubFile
Journal Article
DOI
10.1107/S1600536812046326 [doi]
Output Language
Unknown(0)
PMID
23476231
Abstract
The asymmetric unit of the title phospho-ric triamide, C13H21FN3O2P, consists of two independent mol-ecules. In each mol-ecule, the P=O group and the N-H unit belonging to the C(O)NHP(O) fragment are in a syn conformation with respect to each other. An intra-molecular N-Hcdots, three dots, centeredO hydrogen bond occurs in each mol-ecule. The P atom adopts a distorted tetra-hedral environment. The methyl groups of an isopropyl fragment are disordered over two sets of sites with refined occupancies of 0.458 (5) and 0.542 (5). In the crystal, mol-ecules are linked through N-Hcdots, three dots, centeredO(=P) and N-Hcdots, three dots, centeredO(=C) hydrogen bonds into chains along [001].
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Pourayoubi,M., Tarahhomi,A., Rheingold,A.L., Golen,J.A.
Original/Translated Title
URL
Date of Electronic
20121124
PMCID
PMC3588995
Editors