Image
WTS Database
The subject of interest is:
waterpipe, water-pipe, nargile, narghile, arguileh, arguile, shisha, sheesha, chichi, hubble bubble, hubbly bubbly, goza, hookah, bong
| Title | Pub Year | Author Sort descending | SearchLink |
|---|---|---|---|
| Biodiversity of lactic acid bacteria in Moroccan soft white cheese (Jben) | 2005 | Laboratoire de Microbiologie et Biologie Moleculaire, Centre National pour la Recherche Scientifique et Technique, Laboratory of microbiology and Molecular Biology (LMBM), 52. bd Omar Ibn Khattab, BP 8027-10102 Agdal, Rabat, Morocco. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
FEMS microbiology letters
Periodical, Abbrev.
FEMS Microbiol.Lett.
Pub Date Free Form
15-Oct
Volume
251
Issue
2
Start Page
267
Other Pages
271
Notes
LR: 20061115; JID: 7705721; 0 (DNA, Bacterial); 2005/04/14 [received]; 2005/06/09 [revised]; 2005/08/09 [accepted]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
0378-1097; 0378-1097
Accession Number
PMID: 16168579
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
S0378-1097(05)00559-8 [pii]
Output Language
Unknown(0)
PMID
16168579
Abstract
The bacterial diversity occurring in traditional Moroccan soft white cheese, produced in eight different regions in Morocco, was studied. A total of 164 lactic acid bacteria were isolated, purified and identified by whole-cell protein fingerprinting and rep-PCR genomic fingerprinting. The majority of the strains belonged to the genera Lactobacillus, Lactococcus, Leuconostoc and Enterococcus. Sixteen species were identified: Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus brevis, Lactobacillus buchneri, Lactococcus lactis, Lactococcus garvieae, Lactococcus raffinolactis, Leuconostoc pseudomesenteroides, Leuconostoc mesenteroides, Leuconostoc citreum, Eterococcus durans, Enterococcus faecalis, Enterococcus faecium, Enterococcus saccharominimus and Streptococcus sp.
Descriptors
Biodiversity, Cheese/microbiology, DNA Fingerprinting, DNA, Bacterial/analysis, Food Microbiology, Lactobacillus/genetics/isolation & purification, Lactococcus/genetics/isolation & purification, Leuconostoc/genetics/isolation & purification, Morocco, Polymerase Chain Reaction, Sequence Analysis, DNA
Links
Book Title
Database
Publisher
Data Source
Authors
Ouadghiri,M., Amar,M., Vancanneyt,M., Swings,J.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
|
|||
| Molecular epidemiology of extended-spectrum beta-lactamase-producing Escherichia coli in Tunisia and characterization of their virulence factors and plasmid addiction systems | 2013 | Laboratoire de Microbiologie, Hopital Habib Bourguiba, Sfax, Tunisie. basma_mnif@yahoo.fr | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
BMC microbiology
Periodical, Abbrev.
BMC Microbiol.
Pub Date Free Form
25-Jun
Volume
13
Issue
Start Page
147
Other Pages
2180-13-147
Notes
LR: 20150423; JID: 100966981; 0 (Virulence Factors); EC 3.5.2.6 (beta-Lactamases); OID: NLM: PMC3701463; 2013/03/28 [received]; 2013/05/27 [accepted]; 2013/06/25 [aheadofprint]; epublish
Place of Publication
England
ISSN/ISBN
1471-2180; 1471-2180
Accession Number
PMID: 23800277
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1186/1471-2180-13-147 [doi]
Output Language
Unknown(0)
PMID
23800277
Abstract
BACKGROUND: Extended-spectrum beta-lactamases (ESBLs), particularly CTX-M- type ESBLs, are among the most important resistance determinants spreading worldwide in Enterobacteriaceae. The aim of this study was to characterize a collection of 163 ESBL-producing Escherichia coli collected in Tunisia, their ESBL-encoding plasmids and plasmid associated addiction systems. RESULTS: The collection comprised 163 ESBL producers collected from two university hospitals of Sfax between 1989 and 2009. 118 isolates harbored blaCTX-M gene (101 blaCTX-M-15 gene and 17 blaCTX-M-14 gene). 49 isolates carried blaSHV-12 gene, 9 blaSHV-2a gene and only 3 blaTEM-26 gene. 16 isolates produced both CTX-M and SHV-12. The 101 CTX-M-15-producing isolates were significantly associated to phylogroup B2 and exhibiting a high number of virulence factors. 24 (23.7%) of the group B2 isolates belonged to clonal complex ST131. Pulsed-field gel electrophoresis (PFGE) typing revealed a genetic diversity of the isolates. 144 ESBL determinants were transferable mostly by conjugation. The majority of plasmid carrying blaCTX-M-15 genes (72/88) were assigned to various single replicon or multireplicon IncF types and had significantly a higher frequency of addiction systems, notably the VagCD module. CONCLUSION: This study demonstrates that the dissemination of CTX-M-15 producing E. coli in our setting was due to the spread of various IncF-type plasmids harboring multiple addiction systems, into related clones with high frequency of virulence determinants.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Mnif,B., Harhour,H., Jdidi,J., Mahjoubi,F., Genel,N., Arlet,G., Hammami,A.
Original/Translated Title
URL
Date of Electronic
20130625
PMCID
PMC3701463
Editors
|
|||
| Anti-metabolic activity of caspofungin against Candida albicans and Candida parapsilosis biofilms | 2005 | Laboratoire de Parasitologie et Mycologie Medicales, Centre Hospitalier Universitaire La Miletrie, BP 577, 86021 Poitiers Cedex, France. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
The Journal of antimicrobial chemotherapy
Periodical, Abbrev.
J.Antimicrob.Chemother.
Pub Date Free Form
Sep
Volume
56
Issue
3
Start Page
507
Other Pages
512
Notes
LR: 20131121; JID: 7513617; 0 (Antifungal Agents); 0 (Antimetabolites); 0 (Echinocandins); 0 (Peptides, Cyclic); 8VZV102JFY (Fluconazole); F0XDI6ZL63 (caspofungin); 2005/07/22 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
0305-7453; 0305-7453
Accession Number
PMID: 16040622
Language
eng
SubFile
Journal Article; IM
DOI
dki269 [pii]
Output Language
Unknown(0)
PMID
16040622
Abstract
OBJECTIVES: Candidiasis can be associated with the formation of biofilms on bioprosthetic surfaces and the intrinsic resistance of Candida albicans biofilms to the most commonly used antifungal agents has been demonstrated. In this study, we report on the antifungal activity of caspofungin at two different concentrations, on C. albicans and Candida parapsilosis biofilms with different ages of maturation. METHODS: Fifteen strains of C. albicans (10 strains susceptible to fluconazole in vitro and five strains resistant to this antifungal agent) and six strains of C. parapsilosis (all were susceptible to fluconazole in vitro) were studied. The antifungal activity of caspofungin was assessed by looking for a significant inhibition of the metabolic activity of yeasts within biofilms. Biofilms of Candida were produced in vitro, on silicone catheters. RESULTS: Caspofungin used at MIC did not modify the metabolic activity of C. albicans, whatever the maturation age of the biofilms. The same concentration of caspofungin significantly reduced the metabolism (P<or=0.001) of 25% (biofilms of 48 h) to 50% (biofilms of 2 h) of the C. parapsilosis yeasts. The use of a therapeutic concentration of caspofungin (2 mg/L) significantly decreased (P<or=0.001) the metabolism of all the strains of C. albicans and C. parapsilosis tested, independently of the biofilm maturation age. This potent antifungal activity of caspofungin on C. albicans biofilms was observed independently of the yeast susceptibility to fluconazole. CONCLUSIONS: This study demonstrated that caspofungin used at MIC was not sufficient to reduce C. albicans biofilms, but it suggested an activity on C. parapsilosis biofilms depending on their maturation age. This study also indicated that caspofungin used at 2 mg/L could be a good candidate in the prevention of candidiasis associated with silicone medical devices. Our results also suggested that fluconazole resistance of yeasts did not affect caspofungin activity.
Descriptors
Antifungal Agents/pharmacology, Antimetabolites/pharmacology, Biofilms/drug effects/growth & development, Candida/drug effects/physiology, Candida albicans, Dose-Response Relationship, Drug, Drug Resistance, Multiple, Fungal, Echinocandins, Fluconazole/pharmacology, Microbial Sensitivity Tests, Peptides, Cyclic/pharmacology
Links
Book Title
Database
Publisher
Data Source
Authors
Cocuaud,C., Rodier,M. H., Daniault,G., Imbert,C.
Original/Translated Title
URL
Date of Electronic
20050722
PMCID
Editors
|
|||
| Determination of benzene, toluene, ethylbenzene and xylenes in air by solid phase micro-extraction/gas chromatography/mass spectrometry | 2004 | Laboratoire de Radiochimie, Sciences Analytiques et Environnement, Faculte des Sciences, Universite de Nice-Sophia Antipolis, Parc Valrose, 06108 Nice, France. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Analytical and bioanalytical chemistry
Periodical, Abbrev.
Anal.Bioanal Chem.
Pub Date Free Form
Nov
Volume
380
Issue
6-May
Start Page
824
Other Pages
830
Notes
LR: 20160512; JID: 101134327; 0 (Air Pollutants); 0 (Benzene Derivatives); 0 (Xylenes); 3FPU23BG52 (Toluene); J64922108F (Benzene); L5I45M5G0O (ethylbenzene); ppublish
Place of Publication
Germany
ISSN/ISBN
1618-2642; 1618-2642
Accession Number
PMID: 15517200
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1007/s00216-004-2837-1 [doi]
Output Language
Unknown(0)
PMID
15517200
Abstract
The aim of the study was to analyse BTEX compounds (benzene, toluene, ethylbenzene, xylenes) in air by solid phase micro-extraction/gas chromatography/mass spectrometry (SPME/GC/MS), and this article presents the features of the calibration method proposed. Examples of real-world air analysis are given. Standard gaseous mixtures of BTEX in air were generated by dynamic dilution. SPME sampling was carried out under non-equilibrium conditions using a Carboxen/PDMS fibre exposed for 30 min to standard gas mixtures or to ambient air. The behaviour of the analytical response was studied from 0 to 65 microg/m3 by adding increasing amounts of BTEX to the air matrix. Detection limits range from 0.05 to 0.1 microg/m3 for benzene, depending on the fibre. Inter-fibre relative standard deviations (reproducibility) are larger than 18%, although the repeatability for an individual fibre is better than 10%. Therefore, each fibre should be considered to be a particular sampling device, and characterised individually depending on the required accuracy. Sampling indoor and outdoor air by SPME appears to be a suitable short-delay diagnostic method for volatile organic compounds, taking advantage of short sampling time and simplicity.
Descriptors
Air Pollutants/analysis, Benzene/analysis, Benzene Derivatives/analysis, Calibration, Environmental Monitoring, Gas Chromatography-Mass Spectrometry/methods, Time Factors, Toluene/analysis, Xylenes/analysis
Links
Book Title
Database
Publisher
Data Source
Authors
Tumbiolo,S., Gal,J. F., Maria,P. C., Zerbinati,O.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
|
|||
| Substance use among Lebanese university students: prevalence and associated factors | 2015 | Laboratoire de recherche clinique et epidemiologique, Faculte de Pharmacie, Universite Libanaise, Beyrouth (Liban); Ecole doctorale des Sciences et de Technologie, Universite Libanaise, Beyrouth (Liban); Faculte de Sante publique, Universite Libanaise, Be | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit
Periodical, Abbrev.
East.Mediterr.Health J.
Pub Date Free Form
27-Aug
Volume
21
Issue
5
Start Page
332
Other Pages
341
Notes
JID: 9608387; 2013/02/14 [received]; 2013/12/08 [accepted]; epublish
Place of Publication
Egypt
ISSN/ISBN
1020-3397; 1020-3397
Accession Number
PMID: 26343122
Language
fre
SubFile
English Abstract; Journal Article; Observational Study; IM
DOI
Output Language
Unknown(0)
PMID
26343122
Abstract
Scientific research on use and misuse of substances in Lebanon is scarce. This study aimed to evaluate the rate of use and abuse of substances among Lebanese youth and identify the determinants and risk factors behind these behaviours. An observational survey was conducted on 1945 university students selected from the different faculties of the Lebanese University and other private universities. A self-administered questionnaire based on ASSIST (Alcohol, Smoking and Substance Involvement Screening Test) was administered. The prevalence of ever consuming alcohol was 20.9%. Cannabis (12.3%) and tranquilizers (11%) had the highest rates of ever use among the drugs, whereas cocaine (3.3%) and hallucinogens (3.6%) had the lowest rates. Smoking cigarettes and waterpipes, going out at night, peer pressure and having no specific leisure time activity were associated with problematic substance use, while a better relationship with parents, reading and working were inversely associated with use. There is a high prevalence of substance use among university students in Lebanon. Multidisciplinary support for addicted students is needed to meet their diverse needs.; Publisher: Abstract available from the publisher.; Publisher: Abstract available from the publisher.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Salameh,P., Rachidi,S., Al-Hajje,A., Awada,S., Chouaib,K., Saleh,N., Bawab,W.
Original/Translated Title
Consommation de substances psychoactives des etudiants universitaries libanais: prevalence et facteurs associes
URL
Date of Electronic
20150827
PMCID
Editors
|
|||
| Characterization of CTX-M and SHV extended-spectrum beta-lactamases and associated resistance genes in Escherichia coli strains of food samples in Tunisia | 2007 | Laboratoire MBA, Departement de Biologie, Faculte de Sciences de Tunis, Campus Universitaire, 2092 Tunis, Tunisia. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
The Journal of antimicrobial chemotherapy
Periodical, Abbrev.
J.Antimicrob.Chemother.
Pub Date Free Form
Nov
Volume
60
Issue
5
Start Page
1137
Other Pages
1141
Notes
JID: 7513617; 0 (Escherichia coli Proteins); EC 3.5.2.6 (beta-Lactamases); 2007/09/13 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
0305-7453; 0305-7453
Accession Number
PMID: 17855726
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
dkm316 [pii]
Output Language
Unknown(0)
PMID
17855726
Abstract
OBJECTIVES: To assess the occurrence of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli isolates of faecal samples of animals (n = 40) and food samples (n = 38) obtained in Tunisia in 2006, and to characterize the type of ESBLs, their genetic environments and the associated resistance genes. METHODS: Samples were inoculated in supplemented media (2 mg/L cefotaxime) for isolation of broad-spectrum cephalosporin-resistant E. coli isolates (one isolate/sample). ESBLs and their genetic environments as well as integrons and their gene cassette composition were characterized by PCR and sequencing. RESULTS: ESBL-producing E. coli isolates were detected in 10 of the 38 food samples analysed (26%) and in none of the tested animal faecal samples. Genes found were as follows (number of isolates): bla(CTX-M-1) (5), bla(CTX-M-1) + bla(TEM-1b) (1), bla(CTX-M-14) + bla(TEM-1b) (2), bla(CTX-M-8) (1) and bla(SHV-5) (1). All ESBL-positive isolates showed unrelated PFGE patterns. ISEcp1 and IS903 were detected surrounding bla(CTX-M-14), and ISEcp1/IS26 and orf477 surrounding some of the bla(CTX-M-1) genes. Four of the ESBL-positive strains harboured class 1 integrons including different gene cassette combinations. CONCLUSIONS: ESBLs, mainly of the CTX-M class, are detected in E. coli of food origin in Tunisia, being the first time that this mechanism has been detected in food E. coli strains in Africa.
Descriptors
Animals, Cattle, Chickens/microbiology, Drug Resistance, Multiple, Bacterial/genetics, Escherichia coli/drug effects/enzymology/genetics, Escherichia coli Proteins/genetics, Feces/microbiology, Fishes/microbiology, Food Microbiology, Horses/microbiology, Humans, Meat/microbiology, Sheep/microbiology, Tunisia, Turkeys/microbiology, beta-Lactamases/genetics
Links
Book Title
Database
Publisher
Data Source
Authors
Jouini,A., Vinue,L., Slama,K. B., Saenz,Y., Klibi,N., Hammami,S., Boudabous,A., Torres,C.
Original/Translated Title
URL
Date of Electronic
20070913
PMCID
Editors
|
|||
| Prevalence and characterization of extended-spectrum beta-lactamase (ESBL)- and CMY-2-producing Escherichia coli isolates from healthy food-producing animals in Tunisia | 2012 | Laboratoire Microorganismes et Biomolecules Actives, Faculte des Sciences de Tunis, Universite Tunis-El Manar, Tunis, Tunisia. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Foodborne pathogens and disease
Periodical, Abbrev.
Foodborne Pathog.Dis.
Pub Date Free Form
Dec
Volume
9
Issue
12
Start Page
1137
Other Pages
1142
Notes
JID: 101120121; 0 (Anti-Bacterial Agents); EC 3.5.2.6 (beta-Lactamases); 2012/11/29 [aheadofprint]; ppublish
Place of Publication
United States
ISSN/ISBN
1556-7125; 1535-3141
Accession Number
PMID: 23194332
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1089/fpd.2012.1267 [doi]
Output Language
Unknown(0)
PMID
23194332
Abstract
The prevalence of extended-spectrum beta-lactamase (ESBL)- and plasmidic AmpC-beta-lactamase (pAmpC-BL)-producing Escherichia coli isolates has been studied in food-producing animals at the farm level in Tunisia, and recovered isolates were characterized for the presence of other resistance genes and integrons. Eighty fecal samples of food-producing animals (23 sheep, 22 chickens, 22 cattle, six horses, five rabbits, and two dromedaries) were obtained from 35 different farms in Tunisia in 2011. Samples were inoculated onto MacConkey agar plates supplemented with cefotaxime (2 mg/L) for cefotaxime-resistant (CTX(R)) E. coli recovery. CTX(R) E. coli isolates were detected in 11 out of 80 samples (13.8%), and one isolate per sample was further characterized (10 from chickens and one from a dromedary). The 11 CTX(R) isolates were distributed into phylogroups: B1 (five isolates), A (two isolates), D (three isolates), and B2 (one isolate). The following beta-lactamase genes were detected: bla(CTX-M-1) (seven isolates), bla(CTX-M-1)+bla(TEM-135) (one isolate), bla(CTX-M-1)+bla(TEM-1b) (one isolate), and bla(CMY-2) (two isolates). All ESBL- and pAmpC-BL-producing E. coli strains showed unrelated pulsed-field gel electrophoresis patterns. Seven isolates contained class 1 integrons with four gene cassette arrangements: dfrA17-aadA5 (three isolates), dfrA1-aadA1 (two isolates), dfrA15-aadA1 (one isolate), and aadA1 (one isolate). All isolates showed tetracycline resistance and contained the tet(A) +/- tet(B) genes. Virulence genes detected were as follows (number of isolates in parentheses): fimA (10); aer (eight); papC (two); and papGIII, hly, cnf, and bfp (none). Chicken farms constitute a reservoir of ESBL- and pAmpC-BL-producing E. coli isolates of the CTX-M-1 and CMY-2 types that potentially could be transmitted to humans via the food chain or by direct contact.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Ben Sallem,R., Ben Slama,K., Saenz,Y., Rojo-Bezares,B., Estepa,V., Jouini,A., Gharsa,H., Klibi,N., Boudabous,A., Torres,C.
Original/Translated Title
URL
Date of Electronic
20121129
PMCID
Editors
|
|||
| Widespread distribution of CTX-M and plasmid-mediated AmpC beta-lactamases in Escherichia coli from Brazilian chicken meat | 2015 | Laboratorio de Investigacao em Microbiologia Medica, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.; Laboratorio de Investigacao em Microbiologia Medica, Instituto de Microbiologia Paulo de Go | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Memorias do Instituto Oswaldo Cruz
Periodical, Abbrev.
Mem.Inst.Oswaldo Cruz
Pub Date Free Form
Apr
Volume
110
Issue
2
Start Page
249
Other Pages
254
Notes
LR: 20150707; JID: 7502619; 0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Escherichia coli Proteins); EC 3.5.2.6 (AmpC beta-lactamases); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (beta-lactamase CTX-M, E coli); OID: NLM: PMC4489457; 2014/10/20 [rec
Place of Publication
Brazil
ISSN/ISBN
1678-8060; 0074-0276
Accession Number
PMID: 25946250
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1590/0074-02760140389 [doi]
Output Language
Unknown(0)
PMID
25946250
Abstract
The dissemination of plasmid-mediated antimicrobial resistance genes may pose a substantial public health risk. In the present work, the occurrences of blaCTX-M and plasmid-mediated ampC and qnr genes were investigated in Escherichia coli from 16 chicken carcasses produced by four commercial brands in Brazil. Of the brands tested, three were exporters, including one of organic chicken. Our study assessed 136 E. coli isolates that were grouped into 77 distinct biotypes defined by their origin, resistance profiling, the presence of beta-lactamase and plasmid-mediated quinolone resistance genes and enterobacterial repetitive intergenic consensus-polimerase chain reaction typing. The blaCTX-M-15, blaCTX-M-2 and blaCTX-M-8 genes were detected in one, 17 and eight different biotypes, respectively (45 isolates). Twenty-one biotypes (46 isolates) harboured blaCMY-2. Additionally, blaCMY-2 was identified in isolates that also carried either blaCTX-M-2 or blaCTX-M-8. The qnrB and/or qnrS genes occurred in isolates carrying each of the four types of beta-lactamase determinants detected and also in oxyimino-cephalosporin-susceptible strains. Plasmid-mediated extended-spectrum beta-lactamase (ESBL) and AmpC determinants were identified in carcasses from the four brands tested. Notably, this is the first description of blaCTX-M-15 genes in meat or food-producing animals from South America. The blaCTX-M-8, blaCTX-M-15 and blaCMY-2 genes were transferable in conjugation experiments. The findings of the present study indicate that plasmid-mediated ESBL and AmpC-encoding genes are widely distributed in Brazilian chicken meat.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Botelho,L.A., Kraychete,G.B., Costa e Silva,J.L., Regis,D.V., Picao,R.C., Moreira,B.M., Bonelli,R.R.
Original/Translated Title
URL
Date of Electronic
PMCID
PMC4489457
Editors
|
|||
| Simple approach based on ultrasound-assisted emulsification-microextraction for determination of polibrominated flame retardants in water samples by gas chromatography-mass spectrometry | 2009 | Laboratorio de Investigaciones y Servicios Ambientales Mendoza, Centro Cientifico Tecnologico-CONICET-Mendoza, PO Box 131, 5500 Mendoza, Argentina. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of chromatography.A
Periodical, Abbrev.
J.Chromatogr.A
Pub Date Free Form
2-Jan
Volume
1216
Issue
1
Start Page
147
Other Pages
153
Notes
LR: 20131121; JID: 9318488; 0 (Emulsifying Agents); 0 (Flame Retardants); 0 (Halogenated Diphenyl Ethers); 0 (Solvents); 059QF0KO0R (Water); 2008/08/07 [received]; 2008/11/07 [revised]; 2008/11/13 [accepted]; 2008/11/19 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
0021-9673; 0021-9673
Accession Number
PMID: 19054523
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.chroma.2008.11.034 [doi]
Output Language
Unknown(0)
PMID
19054523
Abstract
A simple, efficient, innovative and environmentally friendly analytical technique was successfully applied for the first time for the extraction and preconcentration of polybrominated diphenyl ethers (PBDEs) from water samples. The PBDEs selected for this work were those most commonly found in the literature in natural water samples: 2,2',4,4'-tetraBDE (BDE-47), 2,2',4,4,5-pentaBDE (BDE-99), 2,2',4,4,6-pentaBDE (BDE-100) and 2,2,4,4',5,5'-hexaBDE (BDE-153). The extracted PBDEs were separated and determined by gas chromatography-mass spectrometry (GC-MS). The extraction/preconcentration technique is based on ultrasound-assisted emulsification-microextraction (USAEME) of a water-immiscible solvent in an aqueous medium. Several variables including, solvent type, extraction time, extraction temperature and matrix modifiers were studied and optimized over the relative response the target analytes. Chloroform was used as extraction solvent in the USAEME technique. Under optimum conditions, the target analytes were quantitatively extracted achieving enrichment factors (EF) higher than 319. The detection limits (LODs) of the analytes for the preconcentration of 10 mL sample volume were within the range 1-2 pg mL(-1). The relative standard deviations (RSD) for five replicates at 10 pg mL(-1) concentration level were or = 0.9985. Validation of the methodology was performed by standard addition method at two concentration levels (10 and 50 pg mL(-1)). Recovery values were > or = 96%, which showed a successful robustness of the analytical methodology for determination of picogram per milliliter of PBDEs in water samples. Significant quantities of PBDEs were not found in the analyzed samples.
Descriptors
Analytic Sample Preparation Methods/methods, Emulsifying Agents/chemistry, Environmental Monitoring, Flame Retardants/analysis, Gas Chromatography-Mass Spectrometry, Halogenated Diphenyl Ethers/analysis, Hydrogen-Ion Concentration, Osmolar Concentration, Solvents/chemistry, Sonication, Temperature, Time Factors, Ultrasonics, Water/chemistry
Links
Book Title
Database
Publisher
Data Source
Authors
Fontana,A. R., Wuilloud,R. G., Martinez,L. D., Altamirano,J. C.
Original/Translated Title
URL
Date of Electronic
20081119
PMCID
Editors
|
|||
| Study on mutagenic effects of bisphenol A diglycidyl ether (BADGE) and its derivatives in the Escherichia coli tryptophan reverse mutation assay | 2006 | Laboratorio de Microbioloxia, Instituto de Investigacion e Analises Alimentarias, Universidade de Santiago de Compostela, Campus Sur s/n, 15782 Santiago de Compostela, Spain. mprosaan@usc.es | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Mutation research
Periodical, Abbrev.
Mutat.Res.
Pub Date Free Form
10-Oct
Volume
609
Issue
1
Start Page
11
Other Pages
16
Notes
LR: 20131121; JID: 0400763; 0 (Benzhydryl Compounds); 0 (Carcinogens); 0 (Epoxy Compounds); 8DUH1N11BX (Tryptophan); F3XRM1NX4H (2,2-bis(4-glycidyloxyphenyl)propane); 2005/07/22 [received]; 2006/05/17 [revised]; 2006/05/29 [accepted]; 2006/07/25 [aheadofp
Place of Publication
Netherlands
ISSN/ISBN
0027-5107; 0027-5107
Accession Number
PMID: 16870492
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
S1383-5718(06)00161-6 [pii]
Output Language
Unknown(0)
PMID
16870492
Abstract
The di-epoxy compound bisphenol A diglycidyl ether (BADGE), its first and second hydrolysis products (BADGE.H2O and BADGE.2H2O, respectively) and its bis-chlorohydrin derivative (BADGE.2HCl) were examined for their mutagenicity in the Escherichia coli tryptophan reverse mutation test with strains WP2, WP2uvrA and IC3327. The assays were performed in the presence and absence of exogenous metabolic activation (S9 fraction from rat liver). The di-epoxy compound BADGE was able to induce mutagenic effects in strains WP2uvrA and IC3327 and the epoxy-diol BADGE.H2O also showed a positive response with these strains, although the latter was less potent than the former. On the other hand, the lack of mutagenic activity of BADGE.2H2O and BADGE.2HCl was also demonstrated.
Descriptors
Benzhydryl Compounds, Carcinogens/chemistry/toxicity, Colony Count, Microbial, Dose-Response Relationship, Drug, Epoxy Compounds/chemistry/toxicity, Escherichia coli/drug effects/genetics/growth & development, Molecular Structure, Mutagenicity Tests/methods, Mutation/drug effects/genetics, Tryptophan/genetics
Links
Book Title
Database
Publisher
Data Source
Authors
Sueiro,R. A., Suarez,S., Araujo,M., Garrido,M. J.
Original/Translated Title
URL
Date of Electronic
20060725
PMCID
Editors
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