WTS database

Title	Pub Year	Author Sort descending	SearchLink
Analysis of bacterial community during the fermentation of pulque, a traditional Mexican alcoholic beverage, using a polyphasic approach	2008	Departamento de Ingenieria Celular y Biocatalisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico (UNAM), Avenida Universidad 2001, Col Chamilpa, Cuernavaca, Morelos, Mexico. adelfo@ibt.unam.mx	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full International journal of food microbiology Periodical, Abbrev. Int.J.Food Microbiol. Pub Date Free Form 31-May Volume 124 Issue 2 Start Page 126 Other Pages 134 Notes JID: 8412849; 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); 2007/06/02 [received]; 2007/11/14 [revised]; 2008/03/03 [accepted]; 2008/03/07 [aheadofprint]; ppublish Place of Publication Netherlands ISSN/ISBN 0168-1605; 0168-1605 Accession Number PMID: 18450312 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1016/j.ijfoodmicro.2008.03.003 [doi] Output Language Unknown(0) PMID 18450312 Abstract In this study, the characterization of the bacterial community present during the fermentation of pulque, a traditional Mexican alcoholic beverage from maguey (Agave), was determined for the first time by a polyphasic approach in which both culture and non-culture dependent methods were utilized. The work included the isolation of lactic acid bacteria (LAB), aerobic mesophiles, and 16S rDNA clone libraries from total DNA extracted from the maguey sap (aguamiel) used as substrate, after inoculation with a sample of previously produced pulque and followed by 6-h fermentation. Microbiological diversity results were correlated with fermentation process parameters such as sucrose, glucose, fructose and fermentation product concentrations. In addition, medium rheological behavior analysis and scanning electron microscopy in aguamiel and during pulque fermentation were also performed. Our results showed that both culture and non-culture dependent approaches allowed the detection of several new and previously reported species within the alpha-, gamma-Proteobacteria and Firmicutes. Bacteria diversity in aguamiel was composed by the heterofermentative Leuconostoc citreum, L. mesenteroides, L. kimchi, the gamma-Proteobacteria Erwinia rhapontici, Enterobacter spp. and Acinetobacter radioresistens. Inoculation with previously fermented pulque incorporated to the system microbiota, homofermentative lactobacilli related to Lactobacillus acidophilus, several alpha-Proteobacteria such as Zymomonas mobilis and Acetobacter malorum, other gamma-Proteobacteria and an important amount of yeasts, creating a starting metabolic diversity composed by homofermentative and heterofermentative LAB, acetic and ethanol producing microorganisms. At the end of the fermentation process, the bacterial diversity was mainly composed by the homofermentative Lactobacillus acidophilus, the heterofermentative L. mesenteroides, Lactococcus lactis subsp. lactis and the alpha-Proteobacteria A. malorum. After a 6-h fermentation, 83.27% of total sugars detected after inoculation were consumed (228.4 mM hexose equivalents) and a carbon (C) recovery of 66.18% in fermentation products was estimated. They were produced 284.4 mM C as ethanol, 71.5 mM C as acetic acid and 19 mM C as lactic acid, demonstrating the presence of homo- and heterofermentative, acetic and alcoholic metabolisms in the final product. It was also found, after hydrolysis, that the exopolysaccharide produced during the fermentation was mainly composed by fructose residues, probably inulin or levan. Descriptors Agave/microbiology, Alcoholic Beverages/microbiology, Bacteria/classification/isolation & purification, Cloning, Molecular, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Ecosystem, Fermentation, Mexico, Phylogeny, RNA, Ribosomal, 16S/genetics, Restriction Mapping, Species Specificity Links Book Title Database Publisher Data Source Authors Escalante,A., Giles-Gomez,M., Hernandez,G., Cordova-Aguilar,M. S., Lopez-Munguia,A., Gosset,G., Bolivar,F. Original/Translated Title URL Date of Electronic 20080307 PMCID Editors
Activity of micafungin against Candida biofilms	2009	Departamento de Inmunologia, Microbiologia y Parasitologia, Facultad de Medicina y Odontologia, Universidad del Pais Vasco-Euskal Herriko Unibertsitatea, Bilbao, Vizcaya, Espana. guillermo.quindos@ehu.es	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Revista Iberoamericana De Micologia Periodical, Abbrev. Rev.Iberoam.Micol. Pub Date Free Form 31-Mar Volume 26 Issue 1 Start Page 49 Other Pages 55 Notes LR: 20121115; JID: 9425531; 0 (Antifungal Agents); 0 (Echinocandins); 0 (Lipopeptides); R10H71BSWG (micafungin); RF: 135; 2009/02/06 [received]; 2009/02/12 [accepted]; 2009/05/07 [aheadofprint]; ppublish Place of Publication Spain ISSN/ISBN 1130-1406; 1130-1406 Accession Number PMID: 19463277 Language spa SubFile English Abstract; Journal Article; Research Support, Non-U.S. Gov't; Review; IM DOI 10.1016/S1130-1406(09)70008-7 [doi] Output Language Unknown(0) PMID 19463277 Abstract BACKGROUND: Most recalcitrant infections are associated to colonization and microbial biofilm development. These biofilms are difficult to eliminate by the immune response mechanisms and the current antimicrobial therapy. AIM: To describe the antifungal of micafungin against fungal biofilms based in the scientific and medical literature of recent years. METHODS: We have done a bibliographic retrieval using the scientific terms "micafungin", "activity", "biofilm", "Candida", "Aspergillus", "fungi", "mycos", susceptibility, in PubMed/Medline from the National Library of Medicine from 2006 to 2009. RESULTS: Most current antifungal agents (amphotericin B and fluconazole) and the new azole antifungals have no activity against fungal biofilms. However, micafungin and the rest of echinocandins are very active against Candida albicans, Candida dubliniensis, Candida glabrata, and Candida krusei biofilms but their activities are variable and less strong against Candida tropicalis and Candida parapsilosis biofilms. Moreover, they have not activities against the biofilms of Cryptococcus y Trichosporon. CONCLUSIONS: The activity of micafungin against Candida biofilms gives more strength to its therapeutic indication for candidaemia and invasive candidiasis associated to catheter, prosthesis and other biomedical devices. Descriptors* Antifungal Agents/pharmacology/therapeutic use, Aspergillus/drug effects, Biofilms/drug effects, Candida/drug effects, Candidiasis/drug therapy, Drug Evaluation, Preclinical, Drug Resistance, Fungal, Drug Resistance, Multiple, Fungal, Echinocandins/pharmacology/therapeutic use, Humans, Lipopeptides/pharmacology/therapeutic use, Mycoses/drug therapy, Prosthesis-Related Infections/drug therapy, Species Specificity Links Book Title Database Publisher Data Source Authors Quindos,G., Villar-Vidal,M., Eraso,E. Original/Translated Title Actividad de la micafungina contra las biopeliculas de Candida URL Date of Electronic 20090507 PMCID Editors
Impact of cigarette package health warnings with pictures in Mexico: results from a survey of smokers in Guadalajara	2012	Departamento de Investigaciones sobre Tabaco, Centro de Investigacion en Salud Poblacional, Instituto Nacional de Salud Publica, Cuernavaca, Morelos, Mexico.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Salud publica de Mexico Periodical, Abbrev. Salud Publica Mex. Pub Date Free Form Jun Volume 54 Issue 3 Start Page 254 Other Pages 263 Notes LR: 20121109; GR: P01 CA138389/CA/NCI NIH HHS/United States; JID: 0404371; 2012/03/08 [received]; 2012/04/25 [accepted]; ppublish Place of Publication Mexico ISSN/ISBN 1606-7916; 0036-3634 Accession Number PMID: 22689163 Language spa SubFile English Abstract; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; IM DOI S0036-36342012000300007 [pii] Output Language Unknown(0) PMID 22689163 Abstract OBJECTIVE: Evaluate the impact of the first pictorial health warning labels (HWLs) on cigarette packs in Mexico. MATERIALS AND METHODS: Cross-sectional survey of a representative sample of 1 765 adult smokers from Guadalajara, Mexico, 2010. Logistic regression models were estimated to determine the association between recall of having purchased a pack with a pictorial HWL and psychosocial variables indicating their impact. RESULTS: 58% reported having purchased a pack with one of the pictorial HWLs, and these were considered the exposed population. Exposed smokers reported a greater frequency of thinking about smoking-related risks (34 vs. 25% p=0.003), and thinking about quitting smoking (23 vs. 14% p=0.001). Exposure to pictorial HWLs was also associated with a greater acceptability of HWLs as a means of communicating with smokers (93 vs. 87% p Descriptors Links Book Title Database Publisher Data Source Authors Thrasher,J.F., Perez-Hernandez,R., Arillo-Santillan,E., Barrientos-Gutierrez,I. Original/Translated Title Impacto de las advertencias con pictogramas en las cajetillas de cigarrillos en Mexico: resultados de una encuesta en fumadores de Guadalajara URL Date of Electronic PMCID Editors
ER-YAG laser pretreatment effect on in vitro secondary caries formation around composite restorations	2001	Departamento de Materiales Dentales, Escuela de Odontologia, Universidad de Granada, Spain.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full American Journal of Dentistry Periodical, Abbrev. Am.J.Dent. Pub Date Free Form Feb Volume 14 Issue 1 Start Page 46 Other Pages 49 Notes LR: 20151119; JID: 8806701; 0 (Acetates); 0 (Aluminum Silicates); 0 (Composite Resins); 0 (Phosphoric Acids); 0 (Resin Cements); 0 (Z100 composite resin); 58784XQC3Y (Yttrium); 7631-86-9 (Silicon Dioxide); 77B218D3YE (Erbium); 90881-69-9 (Scotchbond); C6V Place of Publication United States ISSN/ISBN 0894-8275; 0894-8275 Accession Number PMID: 11806480 Language eng SubFile Clinical Trial; Comparative Study; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't; D DOI Output Language Unknown(0) PMID 11806480 Abstract PURPOSE: This in vitro study determined if Er-YAG laser used in instead of acid-etching influenced artificial secondary caries formation in enamel and root surfaces. MATERIALS AND METHODS: Class V cavities were prepared in buccal and lingual surfaces of 10 extracted caries-free molars, with cervical margins in the root surface and occlusal margins in enamel. The specimens were randomly assigned to 2 groups: Group 1: Enamel and dentin etched with 35% phosphoric acid gel (Scotchbond 15s, rinse 10s; n=5 teeth with 2 cavities per specimen, 10 occlusal and 10 root surface margins at caries risk). Group 2: Enamel and dentin surfaces conditioned using a pulsed Er-YAG laser (KAVO) with 2.94 microm wavelength, 250 micros pulse duration, 300 mJ for enamel and 250 mJ for root surface pulse energy, 2 Hz repetition rate, and water cooling (n=5 teeth with 2 cavities per specimen, 10 occlusal and 10 root surface margins at caries risk). The cavity preparations were restored with a wet-bonding technique (Scotchbond 1 adhesive system) and a hybrid resin, light-cured composite (Z100, A3 shade), according to the manufacturer's instructions. Acid-resistant varnish was applied leaving the restoration and a 1 mm rim of adjacent surface enamel and root surface exposed. The specimens were thermocycled (5-50 degrees C, 500 cycles, dwell time 30s). Following artificial caries formation (2.2 mM calcium, 2.2 mM phosphate, 50 mM acetic acid, 5.0 mg/L fluoride, pH 4.25, 10 days), longitudinal sections (3/tooth, 30 occlusal and cervical caries risk sites per group) were taken for polarized light microscopic examination (water imbibition). Primary surface lesion depth and wall lesion frequency was determined and compared between groups (Student's t-test). RESULTS: Er-YAG laser irradiation resulted in a 56% reduction in primary enamel surface lesion depth (116 microm mean depth) when compared with the acid-etched group (263 microm mean depth), and a 39% decrease in root surface lesion depth (194 microm mean depth) compared with that (316 microm mean depth) for acid-etching pretreatment (P0.05) between treatment groups. Descriptors Acetates, Acid Etching, Dental, Aluminum Silicates, Composite Resins/chemistry, Dental Caries/pathology/prevention & control, Dental Caries Susceptibility, Dental Cavity Preparation/classification, Dental Enamel/radiation effects/ultrastructure, Dental Restoration, Permanent, Dentin/radiation effects/ultrastructure, Erbium, Humans, Hydrogen-Ion Concentration, Low-Level Light Therapy, Microscopy, Polarization, Molar, Phosphoric Acids/administration & dosage, Recurrence, Resin Cements/chemistry, Silicon Dioxide, Statistics as Topic, Thermodynamics, Tooth Demineralization/pathology/prevention & control, Tooth Root/radiation effects/ultrastructure, Yttrium, Zirconium Links Book Title Database Publisher Data Source Authors Ceballos,L., Toledano,M., Osorio,R., Garcia-Godoy,F., Flaitz,C., Hicks,J. Original/Translated Title URL Date of Electronic PMCID Editors
Water supply, sanitation and housing in relation to the risk of infant mortality from diarrhoea	1988	Departamento de Medicina Social, Faculdade de Medicina, Universidade Federal, 96001 Pelotas	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full International journal of epidemiology Periodical, Abbrev. Int.J.Epidemiol. Pub Date Free Form 1988/ Volume 17 Issue 3 Start Page 651 Other Pages 654 Notes Place of Publication ISSN/ISBN 0300-5771 Accession Number Language SubFile DOI Output Language Unknown(0) PMID Abstract In a population-based case-control study in the metropolitan areas of Porto Alegre and Pelotas in southern Brazil children dying in infancy from diarrhoea were compared to neighbourhood controls in terms of several social and environmental variables. Factors found to be significantly associated with an increased risk of death from diarrhoea included the non-availability of piped water, the absence of a flush toilet, residence in a poorly built house and household overcrowding. When adjustment was made for confounding variables and the mutual confounding effect of the environmental variables on each other, the only association that remained statistically significant was that with the availability of piped water. The association with poor housing was almost significant (p = 0.052). Compared to those with water piped to their house, those without easy access to piped water were found to be 4.8 times more likely to suffer infant death from diarrhoea (95% confidence interval 1.7 to 13.8) and those with water piped to their plot but not to their house had a 1.5 times greater risk (95% confidence interval 0.8 to 3.0). Descriptors Brazil, child, diarrhea, epidemiology, fatality, housing, human, infant mortality, priority journal, risk, sanitation, water supply Links Book Title Database Embase; MEDLINE Publisher Data Source Embase Authors Victora,C. G., Smith,P. G., Vaughan,J. P., Nobre,L. C., Lombardi,C., Teixeira,A. M. B., Fuchs,S. C., Moreira,L. B., Gigante,L. P., Barros,F. C. Original/Translated Title URL Date of Electronic PMCID Editors
The activity of echinocandins, amphotericin B and voriconazole against fluconazole-susceptible and fluconazole-resistant Brazilian Candida glabrata isolates	2012	Departamento de Microbiologia e Parasitologia, Centro de Ciencias da Saude, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil. debora_alves6@hotmail.com	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Memorias do Instituto Oswaldo Cruz Periodical, Abbrev. Mem.Inst.Oswaldo Cruz Pub Date Free Form May Volume 107 Issue 3 Start Page 433 Other Pages 436 Notes LR: 20141120; JID: 7502619; 0 (Antifungal Agents); 0 (Echinocandins); 0 (Lipopeptides); 0 (Pyrimidines); 0 (Triazoles); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); 9HLM53094I (anidulafungin); F0XDI6ZL63 (caspofungin); JFU09I87TR (Voriconazole); Place of Publication Brazil ISSN/ISBN 1678-8060; 0074-0276 Accession Number PMID: 22510843 Language eng SubFile Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; IM DOI S0074-02762012000300022 [pii] Output Language Unknown(0) PMID 22510843 Abstract The extensive use of azole antifungal agents has promoted the resistance of Candida spp to these drugs. Candida glabrata is a problematic yeast because it presents a high degree of primary or secondary resistance to fluconazole. In Brazil, C. glabrata has been less studied than other species. In this paper, we compared the activity of three major classes of antifungal agents (azoles, echinocandins and polyenes) against fluconazole-susceptible (FS) and fluconazole-resistant (FR) C. glabrata strains. Cross-resistance between fluconazole and voriconazole was remarkable. Among the antifungal agents, the echinocandins were the most effective against FS and FR C. glabrata and micafungin showed the lowest minimal inhibitory concentrations. Descriptors Links Book Title Database Publisher Data Source Authors Mario,D.A., Denardi,L.B., Bandeira,L.A., Antunes,M.S., Santurio,J.M., Severo,L.C., Alves,S.H. Original/Translated Title URL Date of Electronic PMCID Editors
Pyrosequencing vs. culture-dependent approaches to analyze lactic acid bacteria associated to chicha, a traditional maize-based fermented beverage from Northwestern Argentina	2015	Departamento de Microbiologia y Ecologia, Universitat de Valencia (UVEG), Av. Dr. Moliner 50, 46100, Burjassot, Valencia, Spain.; Departamento de Microbiologia y Ecologia, Universitat de Valencia (UVEG), Av. Dr. Moliner 50, 46100, Burjassot, Valencia, Spa	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full International journal of food microbiology Periodical, Abbrev. Int.J.Food Microbiol. Pub Date Free Form 2-Apr Volume 198 Issue Start Page 9 Other Pages 18 Notes CI: Copyright (c) 2014; JID: 8412849; 0 (RNA, Ribosomal, 16S); OTO: NOTNLM; 2014/07/23 [received]; 2014/12/09 [revised]; 2014/12/21 [accepted]; 2014/12/27 [aheadofprint]; ppublish Place of Publication Netherlands ISSN/ISBN 1879-3460; 0168-1605 Accession Number PMID: 25584777 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1016/j.ijfoodmicro.2014.12.027 [doi] Output Language Unknown(0) PMID 25584777 Abstract The diversity of lactic acid bacteria (LAB) associated with chicha, a traditional maize-based fermented alcoholic beverage from Northwestern Argentina, was analyzed using culture-dependent and culture-independent approaches. Samples corresponding to 10 production steps were obtained from two local producers at Maimara (chicha M) and Tumbaya (chicha T). Whereas by culture-dependent approach a few number of species (Lactobacillus plantarum and Weissella viridescens in chicha M, and Enterococcus faecium and Leuconostoc mesenteroides in chicha T) were identified, a higher quantitative distribution of taxa was found in both beverages by pyrosequencing. The relative abundance of OTUs was higher in chicha M than in chicha T; six LAB genera were common for chicha M and T: Enterococcus, Lactococcus, Streptococcus, Weissella, Leuconostoc and Lactobacillus while Pediococcus only was detected in chicha M. Among the 46 identified LAB species, those of Lactobacillus were dominant in both chicha samples, exhibiting the highest diversity, whereas Enterococcus and Leuconostoc were recorded as the second dominant genera in chicha T and M, respectively. Identification at species level showed the predominance of Lb. plantarum, Lactobacillus rossiae, Leuconostoc lactis and W. viridescens in chicha M while Enterococcus hirae, E. faecium, Lc. mesenteroides and Weissella confusa predominated in chicha T samples. In parallel, when presumptive LAB isolates (chicha M: 146; chicha T: 246) recovered from the same samples were identified by ISR-PCR and RAPD-PCR profiles, species-specific PCR and 16S rRNA gene sequencing, most of them were assigned to the Leuconostoc genus (Lc. mesenteroides and Lc. lactis) in chicha M, Lactobacillus, Weissella and Enterococcus being also present. In contrast, chicha T exhibited the presence of Enterococcus and Leuconostoc, E. faecium being the most representative species. Massive sequencing approach was applied for the first time to study the diversity and evolution of microbial communities during chicha manufacture. Although differences in the LAB species profile between the two geographically different chicha productions were observed by culturing, a larger number for predominant LAB species as well as other minorities were revealed by pyrosequencing. The fine molecular inventory achieved by pyrosequencing provided more precise information on LAB population composition than culture-dependent analysis processes. Descriptors Links Book Title Database Publisher Elsevier B.V Data Source Authors Elizaquivel,P., Perez-Cataluna,A., Yepez,A., Aristimuno,C., Jimenez,E., Cocconcelli,P.S., Vignolo,G., Aznar,R. Original/Translated Title URL Date of Electronic 20141227 PMCID Editors
Identification of Carnobacterium, Lactobacillus, Leuconostoc and Pediococcus by rDNA-based techniques	2003	Departamento de Microbiologia y Ecologia, Universitat de Valencia, Spain.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Systematic and applied microbiology Periodical, Abbrev. Syst.Appl.Microbiol. Pub Date Free Form Nov Volume 26 Issue 4 Start Page 546 Other Pages 556 Notes LR: 20061115; GENBANK/AJ575741; GENBANK/AJ575742; GENBANK/AJ575743; GENBANK/AJ575744; GENBANK/AJ575812; GENBANK/AJ576006; GENBANK/AJ576007; GENBANK/AJ576008; GENBANK/AJ576009; JID: 8306133; 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (DNA, Ribosomal Spacer) Place of Publication Germany ISSN/ISBN 0723-2020; 0723-2020 Accession Number PMID: 14666983 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI S0723-2020(04)70221-0 [pii] Output Language Unknown(0) PMID 14666983 Abstract Ribosomal DNA-based techniques including the analysis of profiles generated by ISR amplification, ISR restriction and ARDRA have been evaluated as molecular tools for identifying Carnobacterium, Lactobacillus, Leuconostoc and Pediococcus. They have been applied for the molecular characterization of 91 strains with the following identities: eight Carnobacterium including the eight type species of the genus; 61 Lactobacillus including 40 type strains out of 45 species, 13 Leuconostoc, out of them 11 are type strains and three are subspecies of Lc. mesenteroides; and nine strains representing the six species of genus Pediococcus. The genetic relationship displayed between these species by rrn-based profiles is sustained by their phylogenetic relationships and can therefore be considered useful for taxonomic purposes. Profiles obtained by ISR amplification allowed identification at genus level of Carnobacterium and Leuconostoc, and even at species level in genus Carnobacterium. Genera Lactobacillus and Pediococcus could not be distinguished from each other by applying this technique. The Lactobacillus species analysed here (45) were differentiated using ARDRA-DdeI and ISR-DdeI profiles, sequentially, and Pediococcus species by ISR-DdeI profiles. It was necessary to combine profiles generated by restriction of ISR-DdeI, ARDRA-DdeI and ARDRA-HaeIII in order to complete the identification of Leuconostoc species. Descriptors Bacterial Typing Techniques/methods, DNA, Bacterial/chemistry/genetics/isolation & purification, DNA, Ribosomal/chemistry/genetics/isolation & purification, DNA, Ribosomal Spacer/analysis/isolation & purification, Food Microbiology, Genes, rRNA, Genotype, Gram-Positive Bacteria/classification/genetics/isolation & purification, Lactobacillus/classification/genetics/isolation & purification, Leuconostoc/classification/genetics/isolation & purification, Molecular Sequence Data, Pediococcus/classification/genetics/isolation & purification, Phylogeny, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA Links Book Title Database Publisher Data Source Authors Chenoll,E., Macian,M. C., Aznar,R. Original/Translated Title URL Date of Electronic PMCID Editors
Activity of caspofungin and voriconazole against clinical isolates of Candida and other medically important yeasts by the CLSI M-44A disk diffusion method with Neo-Sensitabs tablets	2008	Departamento de Microbiologia, ACIA, Barcelona, Spain. acarrillo@ya.com	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Chemotherapy Periodical, Abbrev. Chemotherapy Pub Date Free Form Volume 54 Issue 1 Start Page 38 Other Pages 42 Notes LR: 20141120; CI: (c) 2007; JID: 0144731; 0 (Antifungal Agents); 0 (Echinocandins); 0 (Pyrimidines); 0 (Triazoles); 304NUG5GF4 (Itraconazole); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); F0XDI6ZL63 (caspofungin); JFU09I87TR (Voriconazole); R940 Place of Publication Switzerland ISSN/ISBN 1421-9794; 0009-3157 Accession Number PMID: 18073469 Language eng SubFile Comparative Study; Journal Article; IM DOI 000112414 [pii] Output Language Unknown(0) PMID 18073469 Abstract In vitro activity of caspofungin and voriconazole against 184 clinical isolates of Candida and other medically important yeasts in comparison with that of fluconazole, ketoconazole, itraconazole and amphotericin B was determined by using a disk diffusion method (Neo-Sensitabs) standardized according to the recommendations of the CLSI documents M44-A and M44-S1 (same medium: Mueller-Hinton plus methylene blue; inoculum and minimal inhibitory concentration/zone breakpoints). Seventy-two percent of clinical isolates were susceptible to caspofungin, 23.6% showed an intermediate susceptibility (most of them were Candida parapsilosis) and 4.3% were resistant (values for Candida spp. were 71.2, 23.8 and 5%, respectively). For voriconazole, 96.7% of clinical isolates were susceptible and 3.3% were resistant (Candida spp.: 96 and 3.8%, respectively). Both caspofungin and voriconazole showed high activity against a wide variety of clinically important yeasts. Descriptors Amphotericin B/pharmacology, Antifungal Agents/pharmacology, Candida/drug effects, Cryptococcus/drug effects, Disk Diffusion Antimicrobial Tests, Drug Resistance, Fungal, Echinocandins/pharmacology, Fluconazole/pharmacology, Itraconazole/pharmacology, Ketoconazole/pharmacology, Pyrimidines/pharmacology, Rhodotorula/drug effects, Triazoles/pharmacology, Trichosporon/drug effects, Voriconazole Links Book Title Database Publisher S. Karger AG, Basel Data Source Authors Carrillo-Munoz,A. J., Quindos,G., del Valle,O., Santos,P., Giusiano,G., Ezkurra,P. A., Estivill,M. D., Casals,J. B. Original/Translated Title URL Date of Electronic 20071210 PMCID Editors
Polyphasic approach to bacterial dynamics during the ripening of Spanish farmhouse cheese, using culture-dependent and -independent methods	2008	Departamento de Microbiologia, Facultad de Ciencias, Fuentenueva s/n, 18071 Granada, Spain. mmartine@ugr.es	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Applied and Environmental Microbiology Periodical, Abbrev. Appl.Environ.Microbiol. Pub Date Free Form Sep Volume 74 Issue 18 Start Page 5662 Other Pages 5673 Notes LR: 20140903; JID: 7605801; 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); OID: NLM: PMC2547036; 2008/07/25 [aheadofprint]; ppublish Place of Publication United States ISSN/ISBN 1098-5336; 0099-2240 Accession Number PMID: 18658288 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1128/AEM.00418-08 [doi] Output Language Unknown(0) PMID 18658288 Abstract We studied the dynamics of the microbial population during ripening of Cueva de la Magaha cheese using a combination of classical and molecular techniques. Samples taken during ripening of this Spanish goat's milk cheese in which Lactococcus lactis and Streptococcus thermophilus were used as starter cultures were analyzed. All bacterial isolates were clustered by using randomly amplified polymorphic DNA (RAPD) and identified by 16S rRNA gene sequencing, species-specific PCR, and multiplex PCR. Our results indicate that the majority of the 225 strains isolated and enumerated on solid media during the ripening period were nonstarter lactic acid bacteria, and Lactobacillus paracasei was the most abundant species. Other Lactobacillus species, such as Lactobacillus plantarum and Lactobacillus parabuchneri, were also detected at the beginning and end of ripening, respectively. Non-lactic-acid bacteria, mainly Kocuria and Staphylococcus strains, were also detected at the end of the ripening period. Microbial community dynamics determined by temporal temperature gradient gel electrophoresis provided a more precise estimate of the distribution of bacteria and enabled us to detect Lactobacillus curvatus and the starter bacteria S. thermophilus and L. lactis, which were not isolated. Surprisingly, the bacterium most frequently found using culture-dependent analysis, L. paracasei, was scarcely detected by this molecular approach. Finally, we studied the composition of the lactobacilli and their evolution by using length heterogeneity PCR. Descriptors Animals, Biodiversity, Cheese/microbiology, Colony Count, Microbial, DNA Fingerprinting, DNA, Bacterial/genetics, Ecosystem, Electrophoresis, Gel, Pulsed-Field, Food Microbiology, Genotype, Goats, Lactococcus/classification/genetics/growth & development, Milk/microbiology, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics, Random Amplified Polymorphic DNA Technique, Species Specificity, Streptococcus thermophilus/growth & development, Time Factors Links Book Title Database Publisher Data Source Authors Martin-Platero,A. M., Valdivia,E., Maqueda,M., Martin-Sanchez,I., Martinez-Bueno,M. Original/Translated Title URL Date of Electronic 20080725 PMCID PMC2547036 Editors

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