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Shear bond strength of orthodontic brackets after acid-etched and erbium-doped yttrium aluminum garnet laser-etched 2014 Dental Materials Research Center and Department of Orthodontics, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.; Torabinejad Dental Research Center and Department of Periodontics, School of Dentistry, Isfahan University of Med
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Dental research journal
Periodical, Abbrev.
Dent.Res.J.(Isfahan)
Pub Date Free Form
May
Volume
11
Issue
3
Start Page
321
Other Pages
326
Notes
LR: 20140808; JID: 101471186; OID: NLM: PMC4119364; OTO: NOTNLM; ppublish
Place of Publication
Iran
ISSN/ISBN
1735-3327; 1735-3327
Accession Number
PMID: 25097641
Language
eng
SubFile
Journal Article
DOI
Output Language
Unknown(0)
PMID
25097641
Abstract
BACKGROUND: Laser ablation has been suggested as an alternative method to acid etching; however, previous studies have obtained contrasting results. The purpose of this study was to compare the shear bond strength (SBS) and fracture mode of orthodontic brackets that are bonded to enamel etched with acid and erbium-doped yttrium aluminum garnet (Er:YAG) laser. MATERIALS AND METHODS: In this experimental in vitro study, buccal surfaces of 15 non-carious human premolars were divided into mesial and distal regions. Randomly, one of the regions was etched with 37% phosphoric acid for 15 s and another region irradiated with Er:YAG laser at 100 mJ energy and 20 Hz frequency for 20 s. Stainless steel brackets were then bonded using Transbond XT, following which all the samples were stored in distilled water for 24 h and then subjected to 500 thermal cycles. SBS was tested by a chisel edge, mounted on the crosshead of universal testing machine. After debonding, the teeth were examined under x10 magnification and adhesive remnant index (ARI) score determined. SBS and ARI scores of the two groups were then compared using t-test and Mann-Whitney U test. Significant level was set at P
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Alavi,S., Birang,R., Hajizadeh,F.
Original/Translated Title
URL
Date of Electronic
PMCID
PMC4119364
Editors
A serological survey on viral haemorrhagic fevers in Liberia 1982 Dep. Med., Bernhard-Nocht-Inst. Schiffs- Tropenkr., Hamburg
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Annales de Virologie
Periodical, Abbrev.
ANN.VIROL.
Pub Date Free Form
1982/
Volume
133
Issue
2
Start Page
125
Other Pages
128
Notes
Place of Publication
ISSN/ISBN
0242-5017
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
The results demonstrate that Lassa fever is endemic in Liberia. Consistent with the occurrence of active Lassa fever cases, the prevalent Lassa virus activity seems to be in Lofa County and neighbouring Guinea. Statistically, medical personnel exposed to Lassa fever patients are not at a higher risk for Lassa virus infection than the general population. The higher level of contact with active cases incurred by the medical profession may be compensated by the usually higher standards of hygiene in the dwellings of such personnel and a concomitant lowered exposure to the natural host (Mastomys natalensis). Convincing conclusions concerning the endemic areas of the three viruses studied were complicated by the movements of the tested persons within Liberia and neighbouring Guinea. However, the fact that Lassa, Ebola and Marburg virus antibodies never appeared in the same serum sample leads one to theorize that the endemic regions of the three viruses are well-separated from each other. Consistent with this finding is the statistically significant different distribution of Lassa and Ebola virus antibody carriers, at least between Lofa County and Bong County. The broad age distribution of persons tested and the wide range of antibody titres observed for the activity of the three viruses suggest that the respective diseases probably occur more frequently in single cases rather than in occasional nosocomial or local epidemics. Considering that active cases of Ebola or Marburg virus disease have never been reported from Liberia, the occurrence of these specific antibodies was surprising. The results seem to indicate that Liberia has to be included in the Ebola and Marburg virus endemic zone. Therefore, the medical personnel in Liberian health centres should be aware of the possibility that they may come across active cases and thus be prepared to avoid nosocomial epidemics. Recent investigations doubt the specificity of the indirect immunofluorescence antibody test concerning Ebola and Marburg viruses (K.M. Johnson, personal communication). Thus the significance of the antibodies to Ebola and Marburg viruses identified in Liberia requires further evaluation.
Descriptors
virus antibody, epidemiology, geographic distribution, human cell, Lassa virus, Marburgvirus, serology
Links
Book Title
Database
Embase
Publisher
Data Source
Embase
Authors
Knobloch,J., Albiez,E. J., Schmitz,H.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Isolation and characterization of potentially pathogenic antimicrobial-resistant Escherichia coli strains from chicken and pig farms in Spain 2010 Departament de Genetica i de Microbiologia, Universitat Autonoma de Barcelona, 08193 Cerdanyola del Valles, Barcelona, Spain.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Applied and Environmental Microbiology
Periodical, Abbrev.
Appl.Environ.Microbiol.
Pub Date Free Form
May
Volume
76
Issue
9
Start Page
2799
Other Pages
2805
Notes
LR: 20141204; JID: 7605801; EC 3.5.2.- (beta-lactamase TEM-3); EC 3.5.2.6 (beta-Lactamases); OID: NLM: PMC2863447; 2010/03/12 [aheadofprint]; ppublish
Place of Publication
United States
ISSN/ISBN
1098-5336; 0099-2240
Accession Number
PMID: 20228098
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1128/AEM.02421-09 [doi]
Output Language
Unknown(0)
PMID
20228098
Abstract
To ascertain whether on animal farms there reside extended-spectrum beta-lactamase (ESBL) and plasmidic class C beta-lactamase-producing Escherichia coli isolates potentially pathogenic for humans, phylogenetic analyses, pulsed-field gel electrophoresis (PFGE) typing, serotyping, and virulence genotyping were performed for 86 isolates from poultry (57 isolates) and pig (29 isolates) farms. E. coli isolates from poultry farms carried genes encoding enzymes of the CTX-M-9 group as well as CMY-2, whereas those from pig farms mainly carried genes encoding CTX-M-1 enzymes. Poultry and pig isolates differed significantly in their phylogenetic group assignments, with phylogroup A predominating in pig isolates and phylogroup D predominating in avian isolates. Among the 86 farm isolates, 23 (26.7%) carried two or more virulence genes typical of extraintestinal pathogenic E. coli (ExPEC). Of these, 20 were isolated from poultry farms and only 3 from pig farms. Ten of the 23 isolates belonged to the classic human ExPEC serotypes O2:H6, O2:HNM, O2:H7, O15:H1, and O25:H4. Despite the high diversity of serotypes and pulsotypes detected among the 86 farm isolates, 13 PFGE clusters were identified. Four of these clusters contained isolates with two or more virulence genes, and two clusters exhibited the classic human ExPEC serotypes O2:HNM (ST10) and O2:H6 (ST115). Although O2:HNM and O2:H6 isolates of human and animal origins differed with respect to their virulence genes and PFGE pulsotypes, the O2:HNM isolates from pigs showed the same sequence type (ST10) as those from humans. The single avian O15:H1 isolate was compared with human clinical isolates of this serotype. Although all were found to belong to phylogroup D and shared the same virulence gene profile, they differed in their sequence types (ST362-avian and ST393-human) and PFGE pulsotypes. Noteworthy was the detection, for the first time, in poultry farms of the clonal groups O25b:H4-ST131-B2, producing CTX-M-9, and O25a-ST648-D, producing CTX-M-32. The virulence genes and PFGE profiles of these two groups were very similar to those of clinical human isolates. While further studies are required to determine the true zoonotic potential of these clonal groups, our results emphasize the zoonotic risk posed especially by poultry farms, but also by pig farms, as reservoirs of ESBL- and CMY-2-encoding E. coli.
Descriptors
Agriculture, Animals, Chickens, Drug Resistance, Bacterial, Electrophoresis, Gel, Pulsed-Field, Escherichia coli/classification/genetics/isolation & purification, Escherichia coli Infections/microbiology, Humans, Serotyping, Spain, Sus scrofa, beta-Lactamases/genetics
Links
Book Title
Database
Publisher
Data Source
Authors
Cortes,P., Blanc,V., Mora,A., Dahbi,G., Blanco,J. E., Blanco,M., Lopez,C., Andreu,A., Navarro,F., Alonso,M. P., Bou,G., Blanco,J., Llagostera,M.
Original/Translated Title
URL
Date of Electronic
20100312
PMCID
PMC2863447
Editors
Microbial and physiological characterization of weakly amylolytic but fast-growing lactic acid bacteria: a functional role in supporting microbial diversity in pozol, a Mexican fermented maize beverage 2003 Departamento de Alimentos y Biotecnologia, Facultad de Quimica, UNAM, 04510 Mexico D.F., Mexico.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Applied and Environmental Microbiology
Periodical, Abbrev.
Appl.Environ.Microbiol.
Pub Date Free Form
Aug
Volume
69
Issue
8
Start Page
4367
Other Pages
4374
Notes
LR: 20140611; JID: 7605801; 0 (RNA, Ribosomal, 16S); 9005-25-8 (Starch); OID: NLM: PMC169132; ppublish
Place of Publication
United States
ISSN/ISBN
0099-2240; 0099-2240
Accession Number
PMID: 12902217
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
Output Language
Unknown(0)
PMID
12902217
Abstract
Pozol is an acid beverage obtained from the natural fermentation of nixtamal (heat- and alkali-treated maize) dough. The concentration of mono- and disaccharides from maize is reduced during nixtamalization, so that starch is the main carbohydrate available for lactic acid fermentation. In order to provide some basis to understand the role of amylolytic lactic acid bacteria (ALAB) in this fermented food, their diversity and physiological characteristics were determined. Forty amylolytic strains were characterized by phenotypic and molecular taxonomic methods. Four different biotypes were distinguished via ribotyping; Streptococcus bovis strains were found to be predominant. Streptococcus macedonicus, Lactococcus lactis, and Enterococcus sulfureus strains were also identified. S. bovis strain 25124 showed extremely low amylase yield relative to biomass (139 U g [cell dry weight](-1)) and specific rate of amylase production (130.7 U g [cell dry weight](-1) h(-1)). In contrast, it showed a high specific growth rate (0.94 h(-1)) and an efficient energy conversion yield to bacterial cell biomass (0.31 g of biomass g of substrate(-1)). These would confer on the strain a competitive advantage and are the possible reasons for its dominance. Transient accumulation of maltooligosaccharides during fermentation could presumably serve as energy sources for nonamylolytic species in pozol fermentation. This would explain the observed diversity and the dominance of nonamylolytic lactic acid bacteria at the end of fermentation. These results are the first step to understanding the importance of ALAB during pozol fermentation.
Descriptors
Beverages, Colony Count, Microbial, Fermentation, Lactobacillus/metabolism, Lactococcus lactis/metabolism, RNA, Ribosomal, 16S/genetics, Ribotyping, Starch/metabolism, Streptococcus/metabolism, Zea mays/microbiology
Links
Book Title
Database
Publisher
Data Source
Authors
Diaz-Ruiz,G., Guyot,J. P., Ruiz-Teran,F., Morlon-Guyot,J., Wacher,C.
Original/Translated Title
URL
Date of Electronic
PMCID
PMC169132
Editors
Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals 2011 Departamento de Analises Clinicas, Faculdade de Ciencias Farmaceuticas, Universidade Estadual Paulista, Araraquara, SP, Brasil. rehepi@gmail.com
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Memorias do Instituto Oswaldo Cruz
Periodical, Abbrev.
Mem.Inst.Oswaldo Cruz
Pub Date Free Form
Sep
Volume
106
Issue
6
Start Page
646
Other Pages
654
Notes
LR: 20131121; JID: 7502619; 0 (Antifungal Agents); 0 (Hemodialysis Solutions); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); 2011/01/09 [received]; 2011/08/16 [accepted]; ppublish
Place of Publication
Brazil
ISSN/ISBN
1678-8060; 0074-0276
Accession Number
PMID: 22012217
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
S0074-02762011000600002 [pii]
Output Language
Unknown(0)
PMID
22012217
Abstract
Candida parapsilosis, currently divided into three distinct species, proliferates in glucose-rich solutions and has been associated with infections resulting from the use of medical devices made of plastic, an environment common in dialysis centres. The aims of this study were (i) to screen for Candida orthopsilosis and Candida metapsilosis (100 environmental isolates previously identified as C. parapsilosis), (ii) to test the ability of these isolates to form biofilm and (iii) to investigate the in vitro susceptibility of Candida spp biofilms to the antifungal agents, fluconazole (FLC) and amphotericin B (AMB). Isolates were obtained from a hydraulic circuit collected from a haemodialysis unit. Based on molecular criteria, 47 strains were re-identified as C. orthopsilosis and 53 as C. parapsilosis. Analyses using a formazan salt reduction assay and total viable count, together with microscopy studies, revealed that 72 strains were able to form biofilm that was structurally similar, but with minor differences in morphology. A microtitre-based colorimetric assay used to test the susceptibility of fungal biofilms to AMB and FLC demonstrated that the C. parapsilosis complex displayed an increased resistance to these antifungal agents. The results from these analyses may provide a basis for implementing quality controls and monitoring to ensure the microbiological purity of dialysis water, including the presence of yeast.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Pires,R.H., Santos,J.M., Zaia,J.E., Martins,C.H., Mendes-Giannini,M.J.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Effect of environmental factors and fishing effort allocation on catch of the Spotted Eagle Ray Aetobatus narinari (Rajiformes: Myliobatidae) in Southern Gulf of Mexico 2013 Departamento de Aprovechamiento y Manejo de Recursos Acuaticos, El Colegio de la Frontera Sur (ECOSUR), Av. Rancho Poligono 2-A, Ciudad Industrial, Lerma, Campeche, Mexico, C.P. 24500. ocelycz@gmail.com
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Revista de biologia tropical
Periodical, Abbrev.
Rev.Biol.Trop.
Pub Date Free Form
Sep
Volume
61
Issue
3
Start Page
1341
Other Pages
1349
Notes
JID: 0404267; ppublish
Place of Publication
Costa Rica
ISSN/ISBN
0034-7744; 0034-7744
Accession Number
PMID: 24027927
Language
spa
SubFile
English Abstract; Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
24027927
Abstract
Aetobatus narinari represents a fisheries target in Southern Gulf of Mexico, and it is currently considered a Near Threatened species by the IUCN red list. The information available of this batoid fish includes some biological and fishery aspects; nevertheless, little is known about the factors influencing on fishing operations and catches. In order to evaluate the effect of environmental factors and the fishing effort allocation by vessels on the target fishery of A. narinari in this area, a daily basis sampling was carried out on four small-scale vessels, from January to July 2009 (the entire fishing season), in two fishing localities (Campeche and Seybaplaya). A total of 896 rays were recorded from 280 fishing trips. A General Linear Model was used to predict the factors effect on the probability that fishing operations occurred, and on the probability for captures of at least one or three or five rays per vessel-trip. The probability that fishing operations occurred off Campeche was predicted by the lunar cycle, with the highest probability in the new moon period (66%) and a probability smaller than 35% for the other periods. The probability that fishing operations occurred off Seybaplaya was predicted by wind velocity, with higher probabilities at low wind velocity than at high wind velocity, and a 50% probability of fishing operations at 12-15 km/h. Catch rates off Seybaplaya were predicted by the vessel's factor (the effect of fishing effort allocation), the North wind season and sea surface temperature. The probability for captures of at least one and three rays per vessel-trip was predicted by the vessel's factor and the North wind season. One vessel had higher catch probability (83% for at least one ray and 43% for at least three rays) than the others (69 and 70% for at least one ray and 26% for at least three rays), and during the North wind season the catch probability was higher (96% for at least one ray and 72% for at least three rays) than out of that season (68% for at least one ray and 21% for at least three rays). The probability for capture at least five rays per vessel-trip was predicted by the sea surface temperature and the North wind season. At 23 degrees C the catch probability was of 49% and the probability gradually diminished to 4% at 28 degrees C, and during the North wind season the catch probability was higher (40%) than out of that season (7%). This study shows that some environmental factors and fishermen perceptions and experience (fishing effort allocation) influence on the catch rate of A. narinari, and that these factors must be considered in future studies on elasmobranch fisheries, mainly when comparisons between catch rates among seasons or regions are analyzed.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Cuevas,E., Perez,J.C., Mendez,I.
Original/Translated Title
Efecto de factores ambientales y la asignacion del esfuerzo pesquero sobre las capturas de la raya Aetobatus narinari (Rajiformes: Myliobatidae) en el sur del Golfo de Mexico
URL
Date of Electronic
PMCID
Editors
Selection of lactic acid bacteria from Brazilian kefir grains for potential use as starter or probiotic cultures 2015 Departamento de Biologia Geral, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.; Departamento de Microbiologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Anaerobe
Periodical, Abbrev.
Anaerobe
Pub Date Free Form
Apr
Volume
32
Issue
Start Page
70
Other Pages
76
Notes
CI: Copyright (c) 2014; JID: 9505216; 0 (RNA, Ribosomal); OTO: NOTNLM; 2014/09/18 [received]; 2014/12/04 [revised]; 2014/12/22 [accepted]; 2014/12/24 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
1095-8274; 1075-9964
Accession Number
PMID: 25542841
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.anaerobe.2014.12.007 [doi]
Output Language
Unknown(0)
PMID
25542841
Abstract
Brazilian kefir is a homemade fermented beverage that is obtained by incubating milk or a brown sugar solution with kefir grains that contribute their different microbiological compositions. It is highly important to isolate and characterize microorganisms from Brazilian kefir grains to obtain starter cultures for the industrial production of a standardized commercial kefir. Thus, the present study aimed to isolate lactic acid bacteria from eight kefir grains that were propagated in milk or sugar solutions from five different locations in Brazil and to select Lactobacillus isolates based on desirable in vitro probiotic properties. One hundred eight isolates from both substrates were identified by amplified ribosomal DNA restriction analysis and/or 16S rRNA gene sequencing and were determined to belong to the following 11 species from the genera: Lactococcus, Leuconostoc, Lactobacillus (L.), and Oenococcus. Leuconostoc mesenteroides, Lactobacillus kefiri, and Lactobacillus kefiranofaciens were isolated only from milk grains, whereas Lactobacillus perolens, Lactobacillus parafarraginis, Lactobacillus diolivorans, and Oenococcus oeni were isolated exclusively from sugar water grains. When the microbial compositions of four kefir grains were evaluated with culture-independent analyses, L. kefiranofaciens was observed to predominant in milk grains, whereas Lactobacillus hilgardii was most abundant in sugar water kefir. Unfortunately, L. hilgardii was not isolated from any grain, although this bacteria was detected with a culture-independent methodology. Fifty-two isolated Lactobacilli were tested for gastric juice and bile salt tolerance, antagonism against pathogens, antimicrobial resistance, and surface hydrophobicity. Three Lactobacillus strains (L. kefiranofaciens 8U, L. diolivorans 1Z, and Lactobacillus casei 17U) could be classified as potential probiotics. In conclusion, several lactic acid bacteria that could be used in combination with yeasts as starter cultures for both milk kefir and sugar water kefir were characterized, and the functional properties of several of the lactobacilli isolated from the kefir grains were suggestive of their possible use as probiotics in both kefir and other dairy products.
Descriptors
Links
Book Title
Database
Publisher
Elsevier Ltd
Data Source
Authors
Zanirati,D.F., Abatemarco,M.,Jr, Sandes,S.H., Nicoli,J.R., Nunes,A.C., Neumann,E.
Original/Translated Title
URL
Date of Electronic
20141224
PMCID
Editors
Determination of dynamic characteristics of microbiota in a fermented beverage produced by Brazilian Amerindians using culture-dependent and culture-independent methods 2010 Departamento de Biologia, Universidade Federal de Lavras, 37.200-000, Lavras, MG, Brazil.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International journal of food microbiology
Periodical, Abbrev.
Int.J.Food Microbiol.
Pub Date Free Form
15-Jun
Volume
140
Issue
3-Feb
Start Page
225
Other Pages
231
Notes
CI: Copyright 2010; JID: 8412849; 2009/08/22 [received]; 2010/03/02 [revised]; 2010/03/18 [accepted]; 2010/03/27 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
1879-3460; 0168-1605
Accession Number
PMID: 20413168
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.ijfoodmicro.2010.03.029 [doi]
Output Language
Unknown(0)
PMID
20413168
Abstract
Cauim is a fermented beverages prepared by Tapirape Amerindians in Brazil from substrates such as cassava, rice, peanuts, pumpkin, cotton seed and maize. Here we study the microorganisms associated with peanut and rice fermentation using a combination of culture-dependent and -independent methods. The bacterial population varied from 7.4 to 8.4 log CFU/ml. The yeast population varied from 4.0 to 6.6 log CFU/ml. A total of 297 bacteria and yeasts strains were isolated during fermentation, with 198 bacteria and 99 yeast. The Lactobacillus genus was dominant throughout fermentation. Bacteria and yeast community dynamics during the fermentation process were monitored by PCR-DGGE analysis. Both culture-dependent and -independent methods indicated that the bacterial species L. plantarum, L. fermentum, L. paracasei and L. brevis as well as the yeast species P. guilliermondii, K. lactis, Candida sp, R. toruloides and Saccharomyces cerevisiae, were dominant during fermentation. Multivariate analysis of microorganisms during beverage fermentation showed that the microbial community changed during the fermentation process.
Descriptors
Bacteria/classification/genetics/isolation & purification/metabolism, Beverages/microbiology, Brazil, Colony Count, Microbial, Fermentation, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, Yeasts/classification/genetics/isolation & purification/metabolism
Links
Book Title
Database
Publisher
Elsevier B.V
Data Source
Authors
Ramos,C. L., de Almeida,E. G., Pereira,G. V., Cardoso,P. G., Dias,E. S., Schwan,R. F.
Original/Translated Title
URL
Date of Electronic
20100327
PMCID
Editors
Diversity of bacteria and yeast in the naturally fermented cotton seed and rice beverage produced by Brazilian Amerindians 2011 Departamento de Biologia, Universidade Federal de Lavras, Lavras, MG, Brazil.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Food Microbiology
Periodical, Abbrev.
Food Microbiol.
Pub Date Free Form
Oct
Volume
28
Issue
7
Start Page
1380
Other Pages
1386
Notes
LR: 20151119; CI: Copyright (c) 2011; JID: 8601127; 2011/01/07 [received]; 2011/06/11 [revised]; 2011/06/22 [accepted]; 2011/06/29 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
1095-9998; 0740-0020
Accession Number
PMID: 21839389
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.fm.2011.06.012 [doi]
Output Language
Unknown(0)
PMID
21839389
Abstract
Microorganisms associated with the fermentation of cotton seed and rice were studied using a combination of culture-dependent and -independent methods. Samples of the cotton seed and rice beverage were collected every 8 h during the fermentation process for analysis of the microbiota present over 48 h. The lactic acid bacteria (LAB) population reached values of approximately 8.0 log cfu/mL. A total of 162 bacteria and 81 yeast isolates were identified using polyphasic methods. LAB (Lactobacillus plantarum, Lactobacillus vermiforme, Lactobacillus paracasei) were the most frequently isolated bacteria. Bacillus subtilis was present from 16 h until the end of the fermentation process. A decrease in pH value from 6.92 (0 h) to 4.76 (48 h) was observed, and the concentration of lactic acid reached 24 g/L at the end of the fermentation process. DGGE (denaturing gradient gel electrophoresis) was performed to determine the dynamics of the communities of bacteria and yeast, and the analysis revealed a predominance of LAB throughout the fermentation process. No changes were observed in the yeast community. The yeast species detected were Candida parapsilosis, Candida orthopsilosis, Clavispora lusitaniae and Rhodotorula mucilaginosa. Our studies indicate that the DGGE technique combined with a culture-dependent method is required to discern the dynamics in the fermentation of cotton seed and rice.
Descriptors
Links
Book Title
Database
Publisher
Elsevier Ltd
Data Source
Authors
Ramos,C.L., de Almeida,E.G., Freire,A.L., Freitas Schwan,R.
Original/Translated Title
URL
Date of Electronic
20110629
PMCID
Editors
Evaluation of using aluminum sulfate and water-soluble Moringa oleifera seed lectin to reduce turbidity and toxicity of polluted stream water 2016 Departamento de Bioquimica, Centro de Biociencias, Universidade Federal de Pernambuco, 50670-420, Recife, Pernambuco, Brazil; Nucleo de Pesquisas em Ciencias Ambientais e Biotecnologia, Universidade Catolica de Pernambuco, 50050-590, Recife, Pernambuco, B
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Chemosphere
Periodical, Abbrev.
Chemosphere
Pub Date Free Form
12-Aug
Volume
163
Issue
Start Page
133
Other Pages
141
Notes
LR: 20160815; CI: Copyright (c) 2016; JID: 0320657; OTO: NOTNLM; 2016/06/07 [received]; 2016/08/01 [revised]; 2016/08/02 [accepted]; aheadofprint
Place of Publication
ISSN/ISBN
1879-1298; 0045-6535
Accession Number
PMID: 27526060
Language
ENG
SubFile
JOURNAL ARTICLE
DOI
S0045-6535(16)31036-0 [pii]
Output Language
Unknown(0)
PMID
27526060
Abstract
Aluminum salts are used as coagulants in water treatment; however, the exposure to residual aluminum has been associated with human brain lesions. The water-soluble Moringa oleifera lectin (WSMoL), which is extracted with distilled water and isolated by chitin chromatography, has coagulant activity and is able to reduce the concentration of metal ions in aqueous solutions. This study evaluated the potential of using aluminum sulfate and WSMoL to reduce the turbidity and toxicity of water from the Cavouco stream located in Recife, Pernambuco, Brazil. The water sample used (called P1) was collected from the stream source, which was found to be strongly polluted based on physicochemical and water quality analyses, as well as ecotoxicity assays with Artemia salina and seeds of Eruca sativa and Lactuca sativa. The assays combining WSMoL and aluminum sulfate were more efficient than those that used these agents separately. Furthermore, the greatest reduction in turbidity (96.8%) was obtained with the treatment using aluminum sulfate followed by WSMoL, compared to when they were applied simultaneously (91.3%). In addition, aluminum sulfate followed by WSMoL treatment resulted in residual aluminum concentration (0.3 mg/L) that was much lower than that recorded after the treatment using only the salt (35.5 mg/L). The ecotoxicity of P1 was also strongly reduced after the treatments. In summary, the combined use of aluminum sulfate and WSMoL was efficient in promoting a strong reduction of turbidity and ecotoxicity of a polluted water sample, without resulting in a high residual aluminum concentration at the conclusion of the treatment.
Descriptors
Links
Book Title
Database
Publisher
Elsevier Ltd
Data Source
Authors
Freitas,J.H., de Santana,K.V., do Nascimento,A.C., de Paiva,S.C., de Moura,M.C., Coelho,L.C., de Oliveira,M.B., Paiva,P.M., do Nascimento,A.E., Napoleao,T.H.
Original/Translated Title
URL
Date of Electronic
20160812
PMCID
Editors