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Crystal structure of 4-methyl-N-[2-(piperidin-1-yl)eth-yl]benzamide monohydrate 2015 PG and Research Department of Physics, Queen Mary's College, Chennai-4, Tamilnadu, India.; Department of Chemistry, Madras Christian College, Chennai-59, India.; PG and Research Department of Physics, Queen Mary's College, Chennai-4, Tamilnadu, India.; PG
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Journal Article
Periodical, Full
Acta crystallographica.Section E, Crystallographic communications
Periodical, Abbrev.
Acta Crystallogr.E.Crystallogr.Commun.
Pub Date Free Form
30-Apr
Volume
71
Issue
Pt 5
Start Page
o359
Other Pages
60
Notes
LR: 20150524; JID: 101648987; OID: NLM: PMC4420061; OTO: NOTNLM; 2015/05/01 [ecollection]; 2015/03/25 [received]; 2015/04/18 [accepted]; 2015/04/30 [epublish]; epublish
Place of Publication
England
ISSN/ISBN
2056-9890
Accession Number
PMID: 25995948
Language
eng
SubFile
Journal Article
DOI
10.1107/S2056989015007653 [doi]
Output Language
Unknown(0)
PMID
25995948
Abstract
In the title compound, C15H22N2O.H2O, the dihedral angle between the planes of the piperidine and benzene rings is 31.63 (1) degrees . The piperidine ring adopts a chair conformation. The water solvent mol-ecule is involved in inter-species O-Hcdots, three dots, centeredO, O-Hcdots, three dots, centeredN, N-Hcdots, three dots, centeredO and weak C-Hcdots, three dots, centeredO hydrogen-bonding inter-actions, giving rise to chains extending along [010].
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Database
Publisher
Data Source
Authors
Revathi,B.K., Reuben Jonathan,D., Sathya,S., Prathebha,K., Usha,G.
Original/Translated Title
URL
Date of Electronic
20150430
PMCID
PMC4420061
Editors
Crystal structure of bis-[(5-amino-1H-1,2,4-triazol-3-yl-kappaN (4))acetato-kappaO]di-aqua-nickel(II) dihydrate 2014 South-Russia State Technical University Prosveschenya, 132, Novocherkassk, Rostov Region, 346428, Russian Federation.; South-Russia State Technical University Prosveschenya, 132, Novocherkassk, Rostov Region, 346428, Russian Federation.; South-Russia Stat
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Journal Article
Periodical, Full
Acta crystallographica.Section E, Structure reports online
Periodical, Abbrev.
Acta Crystallogr.Sect.E.Struct.Rep.Online
Pub Date Free Form
4-Oct
Volume
70
Issue
Pt 11
Start Page
286
Other Pages
289
Notes
LR: 20141215; JID: 101089178; OID: NLM: PMC4257300; OTO: NOTNLM; 2014/11/01 [ecollection]; 2014/09/19 [received]; 2014/09/26 [accepted]; 2014/10/04 [epublish]; epublish
Place of Publication
United States
ISSN/ISBN
1600-5368; 1600-5368
Accession Number
PMID: 25484724
Language
eng
SubFile
Journal Article
DOI
10.1107/S1600536814021436 [doi]
Output Language
Unknown(0)
PMID
25484724
Abstract
The title compound, [Ni(C4H5N4O2)2(H2O)2].2H2O, represents the first transition metal complex of the novel chelating triazole ligand, 2-(5-amino-1H-1,2,4-triazol-3-yl)acetic acid (ATAA), to be structurally characterized. In the mol-ecule of the title complex, the nickel(II) cation is located on an inversion centre and is coordinated by two water mol-ecules in axial positions and two O and two N atoms from two trans-oriented chelating anions of the deprotonated ATAA ligand, forming a slightly distorted octa-hedron. The trans angles of the octa-hedron are all 180 degrees due to the inversion symmetry of the mol-ecule. The cis-angles are in the range 87.25 (8)-92.75 (8) degrees . The six-membered chelate ring adopts a slightly twisted boat conformation with puckering parameters Q = 0.542 (2) A, Theta = 88.5 (2) and varphi = 15.4 (3) degrees . The mol-ecular conformation is stabilized by intra-molecular N-Hcdots, three dots, centeredO hydrogen bonds between the amino group and the chelating carboxyl-ate O atom of two trans-oriented ligands. In the crystal, the complex mol-ecules and lattice water mol-ecules are linked into a three-dimensional framework by an extensive network of N-Hcdots, three dots, centeredO, O-Hcdots, three dots, centeredO and O-Hcdots, three dots, centeredN hydrogen bonds.
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Database
Publisher
Data Source
Authors
Chernyshev,V.M., Chernysheva,A.V., Abagyan,R.S., Rybakov,V.B.
Original/Translated Title
URL
Date of Electronic
20141004
PMCID
PMC4257300
Editors
Crystal structure of methyl 2-hy-droxy-5-[(4-oxo-4,5-di-hydro-1,3-thia-zol-2-yl)amino]benzoate 2015 Chemistry and Environmental Division, Manchester Metropolitan University, Manchester M1 5GD, England ; Chemistry Department, Faculty of Science, Minia University, 61519 El-Minia, Egypt.; Department of Chemistry, Tulane University, New Orleans, LA 70118, U
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Periodical, Full
Acta crystallographica.Section E, Crystallographic communications
Periodical, Abbrev.
Acta Crystallogr.E.Crystallogr.Commun.
Pub Date Free Form
9-Apr
Volume
71
Issue
Pt 5
Start Page
o282
Other Pages
3
Notes
LR: 20150524; JID: 101648987; OID: NLM: PMC4420109; OTO: NOTNLM; 2015/05/01 [ecollection]; 2015/03/26 [received]; 2015/03/30 [accepted]; 2015/04/09 [epublish]; epublish
Place of Publication
England
ISSN/ISBN
2056-9890
Accession Number
PMID: 25995905
Language
eng
SubFile
Journal Article
DOI
10.1107/S2056989015006416 [doi]
Output Language
Unknown(0)
PMID
25995905
Abstract
The title compound, C11H10N2O4S, crystallized with two independent mol-ecules (A and B) in the asymmetric unit. They differ primarily in the rotational orientation of the five-membered heterocyclic ring. In mol-ecule A this ring is inclined to the benzene ring by 48.17 (8) degrees , while in mol-ecule B the same dihedral angle is 23.07 (8) degrees . In each mol-ecule there is an intra-molecular O-Hcdots, three dots, centeredO hydrogen bond involving the adjacent hydroxyl group and the ester carbonyl O atom. In the crystal, the A mol-ecules are linked via pairs of N-Hcdots, three dots, centeredN hydrogen bonds, forming inversion dimers. These dimers are linked to the B mol-ecules via N-H..O, C-Hcdots, three dots, centeredO and C-Hcdots, three dots, centeredS hydrogen bonds forming corrugated sheets lying parallel to (102).
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Book Title
Database
Publisher
Data Source
Authors
Mohamed,S.K., Mague,J.T., Akkurt,M., Abdu-Allah,H.H., Albayati,M.R.
Original/Translated Title
URL
Date of Electronic
20150409
PMCID
PMC4420109
Editors
Crystal structure of N-[(2S,5R)-4-oxo-2,3-diphenyl-1,3-thia-zinan-5-yl]acetamide 0.375-hydrate 2015 Department of Chemistry, Pennsylvania State University, University Park, PA 16802, USA.; Pennsylvania State University, Schuylkill Campus, 200 University Drive, Schuylkill Haven, PA 17972, USA.; Pennsylvania State University, Schuylkill Campus, 200 Univer
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Journal Article
Periodical, Full
Acta crystallographica.Section E, Crystallographic communications
Periodical, Abbrev.
Acta Crystallogr.E.Crystallogr.Commun.
Pub Date Free Form
1-Jan
Volume
71
Issue
Pt 1
Start Page
62
Other Pages
64
Notes
LR: 20150225; JID: 101648987; OID: NLM: PMC4331885; OTO: NOTNLM; 2015/01/01 [ecollection]; 2014/11/09 [received]; 2014/12/01 [accepted]; epublish
Place of Publication
England
ISSN/ISBN
2056-9890
Accession Number
PMID: 25705452
Language
eng
SubFile
Journal Article
DOI
10.1107/S2056989014026425 [doi]
Output Language
Unknown(0)
PMID
25705452
Abstract
The asymmetric unit of the title compound, C18H18N2O2S.0.375H2O, has two independent organic mol-ecules (A and B) and 3/4 of a water mol-ecule distributed over three sites. In mol-ecule A, the 1,3-thia-zine ring is in a boat conformation, with the C atoms at the 2- and 5-positions out of the plane. The angle between the two phenyl rings is 51.70 (12) degrees . In mol-ecule B, the thia-zine ring is in a half-chair conformation, with the S atom forming the back of the half-chair. The angle between the two phenyl rings is 84.44 (14) degrees . The A mol-ecule features an intra-molecular N-Hcdots, three dots, centeredO hydrogen bond, which closes an S(5) ring motif. In the crystal, the corresponding N-H grouping of the B mol-ecule participates in an inter-molecular hydrogen bond to the A mol-ecule. The A mol-ecule participates in a C-Hcdots, three dots, centeredO inter-action back to the B mol-ecule, whilst the B mol-ecule features an intra-molecular C-Hcdots, three dots, centeredO link, which generates an S(10) loop.
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Book Title
Database
Publisher
Data Source
Authors
Yennawar,H.P., Singh,H., Silverberg,L.J.
Original/Translated Title
URL
Date of Electronic
20150101
PMCID
PMC4331885
Editors
Crystal structure of N-[4-amino-5-cyano-6-(methyl-sulfan-yl)pyridin-2-yl]acetamide hemihydrate 2015 Department of Physics, Faculty of Sciences, Erciyes University, 38039 Kayseri, Turkey.; Department of Chemistry, The University of Tennessee at Chattanooga, Chattanooga, TN 37403, USA.; Chemistry and Environmental Division, Manchester Metropolitan Univers
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Journal Article
Periodical, Full
Acta crystallographica.Section E, Crystallographic communications
Periodical, Abbrev.
Acta Crystallogr.E.Crystallogr.Commun.
Pub Date Free Form
13-Feb
Volume
71
Issue
Pt 3
Start Page
o171
Other Pages
2
Notes
LR: 20150408; JID: 101648987; OID: NLM: PMC4350750; OTO: NOTNLM; 2015/03/01 [ecollection]; 2015/02/04 [received]; 2015/02/06 [accepted]; 2015/02/13 [epublish]; epublish
Place of Publication
England
ISSN/ISBN
2056-9890
Accession Number
PMID: 25844232
Language
eng
SubFile
Journal Article
DOI
10.1107/S205698901500256X [doi]
Output Language
Unknown(0)
PMID
25844232
Abstract
The title compound, C9H10N4OS.0.5H2O, crystallizes with two independent mol-ecules (A and B) in the asymmetric unit, together with a water mol-ecule of crystallization. The acetamide moiety, which has an extended conformation, is inclined to the pyridine ring by 7.95 (16) degrees in mol-ecule A and by 1.77 (16) degrees in mol-ecule B. In the crystal, the A and B mol-ecules are linked by two N-Hcdots, three dots, centeredOcarbon-yl hydrogen bonds, forming a dimer. The dimers are linked via N-Hcdots, three dots, centeredN hydrogen bonds, forming ribbons that are linked by N-Hcdots, three dots, centeredOwater hydrogen bonds to form sheets parallel to (110). The sheets are linked by O-Hcdots, three dots, centeredN hydrogen bonds, forming slabs, and between the slabs there are weak slipped parallel pi-pi inter-actions [inter-centroid distance = 3.734 (2) A, inter-planar distance = 3.3505 (11) A and slippage = 1.648 A], forming a three-dimensional structure.
Descriptors
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Book Title
Database
Publisher
Data Source
Authors
Akkurt,M., Knight,K.S., Mohamed,S.K., Hussein,B.R., Albayati,M.R.
Original/Translated Title
URL
Date of Electronic
20150213
PMCID
PMC4350750
Editors
Crystal structures of trans-di-chlorido-tetra-kis-[1-(2,6-diiso-propyl-phen-yl)-1H-imidazole-kappaN (3)]iron(II), trans-di-bromido-tetra-kis-[1-(2,6-diiso-propyl-phen-yl)-1H-imidazole-kappaN (3)]iron(II) and trans-di-bromido-tetra-kis-[1-(2,6-diiso-propyl 2014 Department of Chemistry, University of Fribourg, Av. de Perolles, CH-1700 Fribourg, Switzerland.; Department of Chemistry, University of Fribourg, Av. de Perolles, CH-1700 Fribourg, Switzerland.; Benefri Crystallography Service, University of Neuchatel, A
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Journal Article
Periodical, Full
Acta crystallographica.Section E, Structure reports online
Periodical, Abbrev.
Acta Crystallogr.Sect.E.Struct.Rep.Online
Pub Date Free Form
19-Jul
Volume
70
Issue
Pt 8
Start Page
72
Other Pages
76
Notes
LR: 20140926; JID: 101089178; OID: NLM: PMC4158527; OTO: NOTNLM; 2014/08/01 [ecollection]; 2014/06/12 [received]; 2014/06/15 [accepted]; 2014/07/19 [epublish]; epublish
Place of Publication
United States
ISSN/ISBN
1600-5368; 1600-5368
Accession Number
PMID: 25249858
Language
eng
SubFile
Journal Article
DOI
10.1107/S1600536814014056 [doi]
Output Language
Unknown(0)
PMID
25249858
Abstract
The title compounds, [FeCl2(C15H20N2)4], (I), [FeBr2(C15H20N2)4], (II), and [FeBr2(C15H20N2)4].2C4H10O, (IIb), respectively, all have triclinic symmetry, with (I) and (II) being isotypic. The Fe(II) atoms in each of the structures are located on an inversion center. They have octa-hedral FeX 2N4 (X = Cl and Br, respectively) coordination spheres with the Fe(II) atom coordinated by two halide ions in a trans arrangement and by the tertiary N atom of four aryl-imidazole ligands [1-(2,6-diiso-propyl-phen-yl)-1H-imidazole] in the equatorial plane. In the two independent ligands, the benzene and imidazole rings are almost normal to one another, with dihedral angles of 88.19 (15) and 79.26 (14) degrees in (I), 87.0 (3) and 79.2 (3) degrees in (II), and 84.71 (11) and 80.58 (13) degrees in (IIb). The imidazole rings of the two independent ligand mol-ecules are inclined to one another by 70.04 (15), 69.3 (3) and 61.55 (12) degrees in (I), (II) and (IIb), respectively, while the benzene rings are inclined to one another by 82.83 (13), 83.0 (2) and 88.16 (12) degrees , respectively. The various dihedral angles involving (IIb) differ slightly from those in (I) and (II), probably due to the close proximity of the diethyl ether solvent mol-ecule. There are a number of C-Hcdots, three dots, centeredhalide hydrogen bonds in each mol-ecule involving the CH groups of the imidazole units. In the structures of compounds (I) and (II), mol-ecules are linked via pairs of C-Hcdots, three dots, centeredhalogen hydrogen bonds, forming chains along the a axis that enclose R 2 (2)(12) ring motifs. The chains are linked by C-Hcdots, three dots, centeredpi inter-actions, forming sheets parallel to (001). In the structure of compound (IIb), mol-ecules are linked via pairs of C-Hcdots, three dots, centeredhalogen hydrogen bonds, forming chains along the b axis, and the diethyl ether solvent mol-ecules are attached to the chains via C-Hcdots, three dots, centeredO hydrogen bonds. The chains are linked by C-Hcdots, three dots, centeredpi inter-actions, forming sheets parallel to (001). In (I) and (II), the methyl groups of an isopropyl group are disordered over two positions [occupancy ratio = 0.727 (13):0.273 (13) and 0.5:0.5, respectively]. In (IIb), one of the ethyl groups of the diethyl ether solvent mol-ecule is disordered over two positions (occupancy ratio = 0.5:0.5).
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Mafua,R., Jenny,T., Labat,G., Neels,A., Stoeckli-Evans,H.
Original/Translated Title
URL
Date of Electronic
20140719
PMCID
PMC4158527
Editors
Crystal structures of two hydrazinecarbo-thio-amide derivatives: (E)-N-ethyl-2-[(4-oxo-4H-chromen-3-yl)methyl-idene]hydrazinecarbo-thio-amide hemi-hydrate and (E)-2-[(4-chloro-2H-chromen-3-yl)methyl-idene]-N-phenyl-hydrazinecarbo-thio-amide 2015 Department of Physics, Ethiraj College for Women (Autonomous), Chennai 600 008, India.; Department of Chemistry, National Institute of Technology, Tiruchirappalli 620 015, India.; Department of Chemistry, National Institute of Technology, Tiruchirappalli
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Journal Article
Periodical, Full
Acta crystallographica.Section E, Crystallographic communications
Periodical, Abbrev.
Acta Crystallogr.E.Crystallogr.Commun.
Pub Date Free Form
21-Feb
Volume
71
Issue
Pt 3
Start Page
305
Other Pages
308
Notes
LR: 20150408; JID: 101648987; OID: NLM: PMC4350706; OTO: NOTNLM; 2015/03/01 [ecollection]; 2015/02/04 [received]; 2015/02/17 [accepted]; 2015/02/21 [epublish]; epublish
Place of Publication
England
ISSN/ISBN
2056-9890
Accession Number
PMID: 25844194
Language
eng
SubFile
Journal Article
DOI
10.1107/S2056989015003369 [doi]
Output Language
Unknown(0)
PMID
25844194
Abstract
The title compounds, C13H13N3O2S.0.5H2O, (I), and C17H14ClN3OS, (II), are hydrazinecarbo-thio-amide derivatives. Compound (I) crystallizes with two independent mol-ecules (A and B) and a water mol-ecule of crystallization in the asymmetric unit. The chromene moiety is essentially planar in mol-ecules A and B, with maximum deviations of 0.028 (3) and 0.016 (3) A, respectively, for the carbonyl C atoms. In (II), the pyran ring of the chromene moiety adopts a screw-boat conformation and the phenyl ring is inclined by 61.18 (9) degrees to its mean plane. In the crystal of (I), bifurcated N-Hcdots, three dots, centeredO and C-Hcdots, three dots, centeredO hydrogen bonds link the two independent mol-ecules forming A-B dimers with two R 2 (1)(6) ring motifs, and R 2 (2)(10) and R 2 (2)(14) ring motifs. In addition to these, the water mol-ecule forms tetra-furcated hydrogen bonds which alternately generate R 4 (4)(12) and R 6 (6)(22) graph-set ring motifs. There are also pi-pi [inter-centroid distances = 3.5648 (14) and 3.6825 (15) A] inter-actions present, leading to the formation of columns along the c-axis direction. In the crystal of (II), mol-ecules are linked by pairs of N-Hcdots, three dots, centeredS hydrogen bonds, forming inversion dimers with an R 2 (2)(8) ring motif. The dimers are linked by C-Hcdots, three dots, centeredpi inter-actions, forming ribbons lying parallel to (210).
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Database
Publisher
Data Source
Authors
Gangadharan,R., Haribabu,J., Karvembu,R., Sethusankar,K.
Original/Translated Title
URL
Date of Electronic
20150221
PMCID
PMC4350706
Editors
CTX-M-137, a hybrid of CTX-M-14-like and CTX-M-15-like beta-lactamases identified in an Escherichia coli clinical isolate 2014 Department of Immunology, Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, China Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangz
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Periodical, Full
The Journal of antimicrobial chemotherapy
Periodical, Abbrev.
J.Antimicrob.Chemother.
Pub Date Free Form
Aug
Volume
69
Issue
8
Start Page
2081
Other Pages
2085
Notes
CI: (c) The Author 2014; GENBANK/KF790923; JID: 7513617; 0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (Mutant Chimeric Proteins); 23521W1S24 (Clavulanic Acid); 9M416Z9QNR (Ceftazidime); EC 3.5.2.- (beta-lactamase CTX-M-14); EC 3.5.2.- (beta-lactamase
Place of Publication
England
ISSN/ISBN
1460-2091; 0305-7453
Accession Number
PMID: 24777903
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1093/jac/dku126 [doi]
Output Language
Unknown(0)
PMID
24777903
Abstract
OBJECTIVES: To characterize a novel CTX-M chimera, CTX-M-137, from Escherichia coli clinical isolates in China. METHODS: Isolates were collected from five hospitals between 22 February 2009 and 20 December 2011. Resistance genes were investigated by PCR. blaCTX-M-137 was cloned and purified for kinetic measurements. Conjugation experiments, S1-PFGE and Southern blotting were performed to study the plasmid harbouring blaCTX-M-137. The genetic environment of blaCTX-M-137 was determined by genomic cloning and sequencing. RESULTS: A total of 247 cephalosporin-resistant E. coli were identified. blaCTX-M group genes were the most prevalent extended-spectrum beta-lactamase (ESBL) genes, with 71 isolates harbouring blaCTX-M-1 group genes and 137 isolates harbouring blaCTX-M-9 group genes. A novel chimera of CTX-M-14-like and CTX-M-15-like ESBLs, designated CTX-M-137, was identified from a 60-year-old man with a urinary tract infection. The N-terminus of CTX-M-137 matched CTX-M-14 and the C-terminus matched CTX-M-15. CTX-M-137 conferred resistance to ceftazidime, cefotaxime and aztreonam. Purified CTX-M-137 showed good hydrolytic activity against ceftazidime and cefotaxime, and was inhibited by clavulanic acid. The blaCTX-M-137 was carried on an approximately 83 kb IncI1 plasmid. blaCTX-M-137 was carried on a complete transposition unit ISEcp1-blaCTX-M-137-Deltaorf477 inserted into yagA, which is part of the IncI1 plasmid backbone. CONCLUSIONS: We identified a novel CTX-M chimera, CTX-M-137, with a CTX-M-14-like N-terminus and a CTX-M-15-like C-terminus. Our findings suggest an ongoing diversification of CTX-M-type ESBLs through recombination events.
Descriptors
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Book Title
Database
Publisher
. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy
Data Source
Authors
Tian,G.B., Huang,Y.M., Fang,Z.L., Qing,Y., Zhang,X.F., Huang,X.
Original/Translated Title
URL
Date of Electronic
20140428
PMCID
Editors
Cultural buffering as a protective factor against electronic cigarette use among Hispanic emergency department patients 2016 Department of Emergency Medicine, University of Southern California Keck School of Medicine, Los Angeles, CA, USA; Department of Preventive Medicine, University of Southern California Keck School of Medicine, Los Angeles, CA, USA. Electronic address: chun
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Journal Article
Periodical, Full
Addictive Behaviors
Periodical, Abbrev.
Addict.Behav.
Pub Date Free Form
Dec
Volume
63
Issue
Start Page
155
Other Pages
160
Notes
CI: Copyright (c) 2016; JID: 7603486; OTO: NOTNLM; 2016/03/28 [received]; 2016/07/21 [revised]; 2016/07/30 [accepted]; 2016/08/01 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
1873-6327; 0306-4603
Accession Number
PMID: 27513593
Language
eng
SubFile
Journal Article; IM
DOI
10.1016/j.addbeh.2016.07.020 [doi]
Output Language
Unknown(0)
PMID
27513593
Abstract
INTRODUCTION: Hispanics in the U.S. historically use tobacco at lower rates than other racial and ethnic groups. Cultural buffering, the process by which aspects of traditional Hispanic culture delay the adoption of unhealthy behaviors, is believed to be a protective factor against tobacco use. Electronic cigarettes (e-cigarettes) are a new tobacco product that have not been extensively studied, and it is unknown if cultural factors that protect against tobacco use will buffer against e-cigarette use among the Hispanic population. METHODS: This cross-sectional study was conducted at the emergency department (ED) in a safety-net hospital in 2014. Patients visiting the ED participated in a survey assessing demographics and substance use. Cultural buffering was operationalized as participants' primary language spoken at home. Multivariate logistic regression and generalized estimating equations examined the association between Hispanic cultural buffering and e-cigarette ever-use. RESULTS: Of the 1476 Hispanic ED patients (age: 46.6M+/-14.5SD, 49.3% male), 7.6% reported e-cigarette ever-use and 11.1% reported current combustible cigarette use. In adjusted models, Spanish speakers were half as likely to report e-cigarette ever-use (O.R.: 0.54, 95% C.I.: 0.34-0.84, p=0.007), compared with English speakers. Combustible cigarette use remained the most significant factor associated with e-cigarette ever-use (O.R.: 9.28, 95% C.I.:7.44-11.56, p
Descriptors
Links
Book Title
Database
Publisher
. Published by Elsevier Ltd
Data Source
Authors
Lam,C.N., Goldenson,N.I., Burner,E., Unger,J.B.
Original/Translated Title
URL
Date of Electronic
20160801
PMCID
Editors
Culture-based and denaturing gradient gel electrophoresis analysis of the bacterial community from Chungkookjang, a traditional Korean fermented soybean food 2012 Division of Biological Science and Technology, Yonsei University, Wonju, Gangwon 220-710, Republic of Korea.
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Journal Article
Periodical, Full
Journal of Food Science
Periodical, Abbrev.
J.Food Sci.
Pub Date Free Form
Oct
Volume
77
Issue
10
Start Page
M572
Other Pages
8
Notes
LR: 20151119; CI: (c) 2012; JID: 0014052; 0 (DNA, Bacterial); 0 (Isoflavones); 0 (RNA, Ribosomal, 16S); 0 (Soybean Proteins); 0 (chungkookjang); 2012/09/05 [aheadofprint]; ppublish
Place of Publication
United States
ISSN/ISBN
1750-3841; 0022-1147
Accession Number
PMID: 22950549
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1111/j.1750-3841.2012.02901.x [doi]
Output Language
Unknown(0)
PMID
22950549
Abstract
The bacterial community of Chungkookjang and raw rice-straw collected from various areas in South Korea was investigated using both culture-dependent and culture-independent methods. Pure cultures were isolated from Chungkookjang and raw rice-straw on tryptic soy agar plates with 72 to 121 colonies and identified by 16S rDNA gene sequence analysis, respectively. The traditional culture-based method and denaturing gradient gel electrophoresis analysis of PCR-amplified 16S rDNA confirmed that Pantoea agglomerans and B. subtilis were identified as predominant in the raw rice-straw and Chungkookjang, respectively, from Iljuk district of Gyeonggi province, P. ananatis and B. licheniformis were identified as predominant in the raw rice-straw and Chungkookjang from Wonju district of Gangwon province, and Microbacterium sp. and B. licheniformis were identified as predominant in the raw rice-straw and Chungkookjang from Sunchang district of Jeolla province. Other strains, such as Bacillus, Enterococcus, Pseudomonas, Rhodococcus, and uncultured bacteria were also present in raw rice-straw and Chungkookjang. PRACTICAL APPLICATION: A comprehensive analysis of these microorganisms would provide a more detailed understanding of the biologically active components of Chungkookjang and help improve its quality. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis can be successfully applied to a fermented food to detect unculturable or more species than the culture-dependent method. This technique is an effective and convenient culture-independent method for studying the bacterial community in Chungkookjang. In this study, the bacterial community of Chungkookjang collected from various areas in South Korea was investigated using both culture-dependent and culture-independent methods.
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Institute of Food Technologists(R)
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Hong,S.W., Choi,J.Y., Chung,K.S.
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20120905
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