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Title	Pub Year	Author	SearchLink Sort ascending
Agreement between exhaled breath carbon monoxide threshold levels and self-reported cigarette smoking in a sample of male adolescents in Jordan	2015	Faculty of Nursing, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan. nasheyab@just.edu.jo.; Department of Public Health, Faculty of Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan. Kkheiral@gmail.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full International journal of environmental research and public health Periodical, Abbrev. Int.J.Environ.Res.Public.Health. Pub Date Free Form 15-Jan Volume 12 Issue 1 Start Page 841 Other Pages 854 Notes LR: 20150203; JID: 101238455; 7U1EE4V452 (Carbon Monoxide); OID: NLM: PMC4306896; 2014/11/22 [received]; -0001/11/30 [revised]; 2015/01/13 [accepted]; epublish Place of Publication Switzerland ISSN/ISBN 1660-4601; 1660-4601 Accession Number PMID: 25599375 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.3390/ijerph120100841 [doi] Output Language Unknown(0) PMID 25599375 Abstract This study aimed to measure the percent agreement between Exhaled Breath Carbon Monoxide (eBCO) measure using a piCO+ smokerlyzer(R) and self-reported cigarette smoking status and to determine the optimal thresholds for definite identification of cigarette smokers of male school students in Jordan. A descriptive, cross sectional, study of a random sample of male adolescents in grades 7 and 8 from four public high schools in Irbid, completed an adaptation of a standardized Arabic-language tobacco smoking questionnaire and an eBCO measure. Sensitivity and specificity of the eBCO were calculated against self-reported cigarette smoking. Participants (n = 439) had a mean age of 12.5 years (SD = 0.50) and 174 (39.9%) reported being an ever smoker of whom 59 (33.9%) reported being a recent (30-day) smoker. The optimal eBCO cut-off point for recent smoking was 4.5 ppm with a sensitivity of 84.7% and specificity of 65.5%. Overall, eBCO can accurately identify recent smokers and distinguish them from non-smokers. The eBCO use enables healthcare professionals and researchers to assess efficacy of smoking cessation and prevention programs without necessarily relying on self-report. Further research is indicated to validate our findings and should be expanded to include females, detailed characteristics of cigarette and waterpipe smoking. Descriptors Links Book Title Database Publisher Data Source Authors Al-Sheyab,N., Kheirallah,K.A., Mangnall,L.J., Gallagher,R. Original/Translated Title URL Date of Electronic 20150115 PMCID PMC4306896 Editors
Decompression sickness in breath-hold diving, and its probable connection to the growth and dissolution of small arterial gas emboli	2015	Department of Chemistry, the Guelph-Waterloo Centre for Graduate Work in Chemistry and the Guelph-Waterloo Physics Institute University of Guelph, Guelph, Ontario N1G 2W1, Canada. Electronic address: sgoldman@uoguelph.ca.; Department of Chemistry, the Gue	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Mathematical biosciences Periodical, Abbrev. Math.Biosci. Pub Date Free Form Apr Volume 262 Issue Start Page 1 Other Pages 9 Notes CI: Copyright (c) 2015; JID: 0103146; OTO: NOTNLM; 2014/10/28 [received]; 2015/01/05 [accepted]; 2015/01/15 [aheadofprint]; ppublish Place of Publication United States ISSN/ISBN 1879-3134; 0025-5564 Accession Number PMID: 25598211 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1016/j.mbs.2015.01.001 [doi] Output Language Unknown(0) PMID 25598211 Abstract We solved the Laplace equation for the radius of an arterial gas embolism (AGE), during and after breath-hold diving. We used a simple three-region diffusion model for the AGE, and applied our results to two types of breath-hold dives: single, very deep competitive-level dives and repetitive shallower breath-hold dives similar to those carried out by indigenous commercial pearl divers in the South Pacific. Because of the effect of surface tension, AGEs tend to dissolve in arterial blood when arteries remote from supersaturated tissue. However if, before fully dissolving, they reach the capillary beds that perfuse the brain and the inner ear, they may become inflated with inert gas that is transferred into them from these contiguous temporarily supersaturated tissues. By using simple kinetic models of cerebral and inner ear tissue, the nitrogen tissue partial pressures during and after the dive(s) were determined. These were used to theoretically calculate AGE growth and dissolution curves for AGEs lodged in capillaries of the brain and inner ear. From these curves it was found that both cerebral and inner ear decompression sickness are expected to occur occasionally in single competitive-level dives. It was also determined from these curves that for the commercial repetitive dives considered, the duration of the surface interval (the time interval separating individual repetitive dives from one another) was a key determinant, as to whether inner ear and/or cerebral decompression sickness arose. Our predictions both for single competitive-level and repetitive commercial breath-hold diving were consistent with what is known about the incidence of cerebral and inner ear decompression sickness in these forms of diving. Descriptors Links Book Title Database Publisher Elsevier Inc Data Source Authors Goldman,S., Solano-Altamirano,J.M. Original/Translated Title URL Date of Electronic 20150115 PMCID Editors
Diversity of lactic acid bacteria isolated from Brazilian water buffalo mozzarella cheese	2015	UNESP-Sao Paulo State Univ, Food Engineering and Technology Dept, Sao Jose do Rio Preto, Brazil.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Journal of Food Science Periodical, Abbrev. J.Food Sci. Pub Date Free Form Feb Volume 80 Issue 2 Start Page M411 Other Pages 7 Notes CI: (c) 2015; JID: 0014052; 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 33X04XA5AT (Lactic Acid); OTO: NOTNLM; 2014/08/17 [received]; 2014/04/12 [accepted]; 2015/01/16 [aheadofprint]; ppublish Place of Publication United States ISSN/ISBN 1750-3841; 0022-1147 Accession Number PMID: 25597646 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1111/1750-3841.12771 [doi] Output Language Unknown(0) PMID 25597646 Abstract The water buffalo mozzarella cheese is a typical Italian cheese which has been introduced in the thriving Brazilian market in the last 10 y, with good acceptance by its consumers. Lactic acid bacteria (LAB) play an important role in the technological and sensory quality of mozzarella cheese. In this study, the aim was to evaluate the diversity of the autochthones viable LAB isolated from water buffalo mozzarella cheese under storage. Samples were collected in 3 independent trials in a dairy industry located in the southeast region of Brazil, on the 28th day of storage, at 4 masculineC. The LAB were characterized by Gram staining, catalase test, capacity to assimilate citrate, and production of CO2 from glucose. The diversity of LAB was evaluated by RAPD-PCR (randomly amplified polymorphic DNA-polymerase chain reaction), 16S rRNA gene sequencing, and by Vitek 2 system. Twenty LAB strains were isolated and clustered into 12 different clusters, and identified as Streptococcus thermophilus, Enterococcus faecium, Enterococcus durans, Leuconostoc mesenteroides subsp. mesenteroides, Lactobacillus fermentum, Lactobacillus casei, Lactobacillus delbrueckii subsp. bulgaricus, and Lactobacillus helveticus. Enterococcus species were dominant and citrate-positive. Only the strains of L. mesenteroides subsp. mesenteroides and L. fermentum produced CO2 from glucose and were citrate-positive, while L. casei was only citrate positive. This is the first report which elucidates the LAB diversity involved in Brazilian water buffalo mozzarella cheese. Furthermore, the results show that despite the absence of natural whey cultures as starters in production, the LAB species identified are the ones typically found in mozzarella cheese. Descriptors Links Book Title Database Publisher Institute of Food Technologists(R) Data Source Authors Silva,L.F., Casella,T., Gomes,E.S., Nogueira,M.C., De Dea Lindner,J., Penna,A.L. Original/Translated Title URL Date of Electronic 20150116 PMCID Editors
The assessment and management of inner ear barotrauma in divers and recommendations for returning to diving	2014	Faculty of Heath Sciences, University of Tasmania, and Medical Co-directors, Department of Diving and Hyperbaric Medicine, Royal Hobart Hospital, PO Box 744, North Hobart, Tasmania 7002, Australia, Phone: +61-(0)414-344744, Fax: +61-(0)3-6222-7268, E-mail	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Diving and hyperbaric medicine Periodical, Abbrev. Diving.Hyperb.Med. Pub Date Free Form Dec Volume 44 Issue 4 Start Page 208 Other Pages 222 Notes JID: 101282742; OTO: NOTNLM; 2014/07/06 [received]; 2014/10/15 [accepted]; ppublish Place of Publication Australia ISSN/ISBN 1833-3516; 1833-3516 Accession Number PMID: 25596834 Language eng SubFile Journal Article; Review; IM DOI Output Language Unknown(0) PMID 25596834 Abstract Inner ear barotrauma (IEBt) constitutes a spectrum of pressure-related pathology in the inner ear, with antecedent middle ear barotrauma (MEBt) common. IEBt includes perilymph fistula, intralabyrinthine membrane tear, inner ear haemorrhage and other rarer pathologies. Following a literature search, the pathophysiology, diagnosis, and treatment of IEBt in divers and best-practice recommendations for returning to diving were reviewed. Sixty-nine papers/texts were identified and 54 accessed. Twenty-five case series (majority surgical) provided guidance on diagnostic pathways; nine solely reported divers. IEBt in divers may be difficult to distinguish from inner ear decompression sickness (IEDCS), and requires dive-risk stratification and careful interrogation regarding diving-related ear events, clinical assessment, pure tone audiometry, a fistula test and electronystagmography (ENG). Once diagnosed, conservative management is the recommended first line therapy for IEBt. Recompression does not appear to cause harm if the diagnosis (IEBt vs IEDCS) is doubtful (limited case data). Exploratory surgery is indicated for severe or persisting vestibular symptoms or hearing loss, deterioration of symptoms, or lack of improvement over 10 days indicating significant pathology. Steroids are used, but without high-level evidence. It may be possible for divers to return to subaquatic activity after stakeholder risk acceptance and informed consent, provided: (1) sensorineural hearing loss is stable and not severe; (2) there is no vestibular involvement (via ENG); (3) high-resolution computed tomography has excluded anatomical predilection to IEBt and (4) education on equalising techniques is provided. There is a need for a prospective data registry and controlled trials to better evaluate diagnostic and treatment algorithms. Descriptors Links Book Title Database Publisher Data Source Authors Elliott,E.J., Smart,D.R. Original/Translated Title URL Date of Electronic PMCID Editors
Explaining the effects of electronic cigarettes on craving for tobacco in recent quitters	2015	Institute of Global Health, Faculty of Medicine, University of Geneva, CH-1211 Geneva 4, Switzerland. Electronic address: Jean-Francois.Etter@unige.ch.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Drug and alcohol dependence Periodical, Abbrev. Drug Alcohol Depend. Pub Date Free Form 1-Mar Volume 148 Issue Start Page 102 Other Pages 108 Notes CI: Copyright (c) 2015; JID: 7513587; 6M3C89ZY6R (Nicotine); OTO: NOTNLM; 2014/10/23 [received]; 2014/12/17 [revised]; 2014/12/20 [accepted]; 2015/01/03 [aheadofprint]; ppublish Place of Publication Ireland ISSN/ISBN 1879-0046; 0376-8716 Accession Number PMID: 25592454 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1016/j.drugalcdep.2014.12.030 [doi] Output Language Unknown(0) PMID 25592454 Abstract OBJECTIVE: To explore how e-cigarettes attenuate craving for tobacco, in e-cigarette users who recently quit smoking. DESIGN: Cross-sectional survey of recent quitters, Internet (French and English), 2012-2014. Participants were 374 daily users of e-cigarettes who had quit smoking in the previous two months, enrolled on websites dedicated to e-cigarettes and to smoking cessation. We measured perception that e-cigarettes attenuate craving for tobacco cigarettes, characteristics of e-cigarettes, modifications of the devices, patterns of e-cigarette use, reasons for use, satisfaction with e-cigarettes, dependence on e-cigarettes, and personal characteristics. RESULTS: The strongest attenuation of craving for tobacco was obtained by using higher nicotine concentrations in refill liquids, modular systems (rather than unmodified devices), and high voltage batteries. The strength of the effect of e-cigarettes on craving was also associated with more intensive use (more puffs per day, more refill liquid). Stronger effects on craving were associated with satisfaction with e-cigarettes, and with reporting that e-cigarettes helped to quit smoking. Participants who reported the strongest effects on craving for tobacco were the most dependent on the e-cigarette and had the strongest urges to vape. CONCLUSIONS: From a public health perspective, there is a trade-off between e-cigarettes that provide high levels of nicotine, high satisfaction and more effects on craving for tobacco, but may also be addictive, and e-cigarettes that contain less nicotine and are less addictive, but are also less satisfactory and less efficient at relieving craving and at helping dependent smokers quit smoking. This trade-off must be kept in mind when regulating e-cigarettes. Descriptors Links Book Title Database Publisher Elsevier Ireland Ltd Data Source Authors Etter,J.F. Original/Translated Title URL Date of Electronic 20150103 PMCID Editors
Use of E-Cigarettes Among Current Smokers: Associations Among Reasons for Use, Quit Intentions, and Current Tobacco Use	2015	Robert D. and Patricia E. Kern Center for the Science of Health Care Delivery, Mayo Clinic, Rochester, MN; rutten.lila@mayo.edu.; Division of Cancer Control and Population Sciences, National Cancer Institute, Bethesda, MD;; Robert D. and Patricia E. Kern	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco Periodical, Abbrev. Nicotine Tob.Res. Pub Date Free Form Oct Volume 17 Issue 10 Start Page 1228 Other Pages 1234 Notes LR: 20160805; CI: (c) The Author 2015; GR: P30 CA015083/CA/NCI NIH HHS/United States; GR: R01CA128638/CA/NCI NIH HHS/United States; JID: 9815751; OID: NLM: PMC4592339 [Available on 10/01/16]; PMCR: 2016/10/01 00:00; 2014/10/14 [received]; 2014/12/29 [acce Place of Publication England ISSN/ISBN 1469-994X; 1462-2203 Accession Number PMID: 25589678 Language eng SubFile Journal Article; Research Support, N.I.H., Extramural; IM DOI 10.1093/ntr/ntv003 [doi] Output Language Unknown(0) PMID 25589678 Abstract INTRODUCTION: Research has documented growing availability and use of e-cigarettes in the United States over the last decade. METHODS: We conducted a national panel survey of current adult cigarette smokers to assess attitudes, beliefs, and behaviors relating to e-cigarette use in the United States (N = 2,254). RESULTS: Among current cigarette smokers, 20.4% reported current use of e-cigarettes on some days and 3.7% reported daily use. Reported reasons for e-cigarette use included: quit smoking (58.4%), reduce smoking (57.9%), and reduce health risks (51.9%). No significant differences in sociodemographic characteristics between e-cigarette users and nonusers were observed. Prior quit attempts were reported more frequently among e-cigarette users (82.8%) than nonusers (74.0%). Intention to quit was reported more frequently among e-cigarette users (64.7%) than nonusers (46.8%). Smokers intending to quit were more likely to be e-cigarette users than those not intending to quit (odds ratio [OR] = 1.90, CI =1.36-2.65). Those who used e-cigarettes to try to quit smoking (OR = 2.25, CI = 1.25-4.05), reduce stress (OR = 3.66, CI = 1.11-12.09), or because they cost less (OR = 3.42, CI = 1.64-7.13) were more likely to report decreases in cigarette smoking than those who did not indicate these reasons. Smokers who reported using e-cigarettes to quit smoking (OR = 16.25, CI = 8.32-31.74) or reduce stress (OR = 4.30, CI = 1.32-14.09) were significantly more likely to report an intention to quit than those who did not indicate those reasons for using e-cigarettes. CONCLUSIONS: Nearly a quarter of smokers in our study reported e-cigarettes use, primarily motivated by intentions to quit or reduce smoking. These findings identify a clinical and public health opportunity to re-engage smokers in cessation efforts. Descriptors Links Book Title Database Publisher . Published by Oxford University Press on behalf of the Society for Research on Nicotine and Tobacco Data Source Authors Rutten,L.J., Blake,K.D., Agunwamba,A.A., Grana,R.A., Wilson,P.M., Ebbert,J.O., Okamoto,J., Leischow,S.J. Original/Translated Title URL Date of Electronic 20150114 PMCID PMC4592339 Editors
Reactions to FDA-Proposed Graphic Warning Labels Affixed to U.S. Smokers' Cigarette Packs	2015	Health Communication Research Laboratory, Washington University in St. Louis, St. Louis, MO; amcqueen@dom.wustl.edu.; Health Communication Research Laboratory, Washington University in St. Louis, St. Louis, MO;; Health Communication Research Laboratory, W	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco Periodical, Abbrev. Nicotine Tob.Res. Pub Date Free Form Jul Volume 17 Issue 7 Start Page 784 Other Pages 795 Notes LR: 20160701; CI: (c) The Author 2015; GR: P50CA95815-09S1/CA/NCI NIH HHS/United States; JID: 9815751; OID: NLM: PMC4542680; 2014/01/31 [received]; 2014/12/15 [accepted]; 2015/01/14 [aheadofprint]; ppublish Place of Publication England ISSN/ISBN 1469-994X; 1462-2203 Accession Number PMID: 25589676 Language eng SubFile Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; IM DOI 10.1093/ntr/ntu339 [doi] Output Language Unknown(0) PMID 25589676 Abstract INTRODUCTION: Graphic warning labels have been shown to be more effective than text-only labels in increasing attention and perceived health risks, but most U.S. studies have involved single exposures in laboratory or Internet settings. METHODS: We recruited a convenience sample (N = 202) of U.S. adult smokers from population subgroups with higher rates of smoking and smoking-related deaths who had participated in a larger survey about graphic warning labels. Participants were randomized to get 1 of 9 graphic + text labels or a text-only label. Research staff affixed a warning label sticker to participants' cigarette pack(s) at enrollment. Color graphic labels covered slightly more than the lower half of packs. Black and white labels of current U.S. text-only warnings covered the existing side warning to prompt attention to the label (i.e., attention control). Participants received extra stickers of the same label for subsequent packs, and completed 3 telephone interviews in 1 week. RESULTS: Participants reported low avoidance ( Descriptors Links Book Title Database Publisher . Published by Oxford University Press on behalf of the Society for Research on Nicotine and Tobacco Data Source Authors McQueen,A., Kreuter,M.W., Boyum,S., Thompson,V.S., Caburnay,C.A., Waters,E.A., Kaphingst,K.A., Rath,S., Fu,Q. Original/Translated Title URL Date of Electronic 20150114 PMCID PMC4542680 Editors
Hookah Use Predicts Cigarette Smoking Progression Among College Smokers	2015	Department of Psychiatry, University of California, San Diego, CA; Mental Health Care Line, VA San Diego Healthcare System, San Diego, CA; nmdoran@ucsd.edu.; San Diego State University/University of California, San Diego Joint Doctoral Program in Clinical	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco Periodical, Abbrev. Nicotine Tob.Res. Pub Date Free Form Nov Volume 17 Issue 11 Start Page 1347 Other Pages 1353 Notes CI: (c) The Author 2015; JID: 9815751; 2014/09/08 [received]; 2014/12/22 [accepted]; 2015/01/12 [aheadofprint]; ppublish Place of Publication England ISSN/ISBN 1469-994X; 1462-2203 Accession Number PMID: 25586774 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1093/ntr/ntu343 [doi] Output Language Unknown(0) PMID 25586774 Abstract INTRODUCTION: Hookah use is increasingly common among U.S. college students, but little is known regarding the relationship between hookah and cigarette use. The purpose of this study was to test the hypothesis that the added nicotine exposure from hookah use may accelerate the uptake of cigarettes. METHODS: An ethnically diverse sample of college students (n = 256; 43% female) who had smoked cigarettes in the past month completed 2 in-person interviews over 6 months. This study was a secondary analysis of data collected for a longitudinal study of young adult cigarette smoking patterns. Analyses examined 6-month changes in past 30 day cigarettes smoked and number of days smoking, controlling for age, nicotine dependence, marijuana use, and the respective baseline variable for each outcome. RESULTS: Current hookah use (any use in past 30 days) was endorsed by 34% of participants at baseline, while 94% reported lifetime use. Change in past 30 day number of cigarettes (p = .043) and number of smoking days (p = .040) differed significantly between those who did or did not report recent hookah use at baseline. Hookah users reported a greater number of cigarettes smoked at the 6-month follow-up, while nonusers decreased their smoking quantity. For number of smoking days in the past 30, hookah users reported a smaller decrease than nonusers. CONCLUSIONS: Recent hookah use predicted increased cigarette smoking over 6 months in a college sample. These are the first prospective data demonstrating this relationship, indicating the value of developing strategies to prevent hookah use among college students. Descriptors Links Book Title Database Publisher . Published by Oxford University Press on behalf of the Society for Research on Nicotine and Tobacco Data Source Authors Doran,N., Godfrey,K.M., Myers,M.G. Original/Translated Title URL Date of Electronic 20150112 PMCID Editors
"A cold shower": electrical magnetic interference caused by water heater current leakage through shower water pipe inducing ICD shock	2015	University of Foggia, Foggia, Italy.; Ospedali Riuniti, Foggia, Italy.; Ospedali Riuniti, Foggia, Italy.; Ospedali Riuniti, Foggia, Italy.; University of Foggia, Foggia, Italy.; University of Foggia, Foggia, Italy. Electronic address: natale.brunetti@unif	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full International journal of cardiology Periodical, Abbrev. Int.J.Cardiol. Pub Date Free Form 1-Mar Volume 182 Issue Start Page 279 Other Pages 280 Notes JID: 8200291; OTO: NOTNLM; 2014/12/05 [received]; 2014/12/29 [accepted]; 2014/12/30 [aheadofprint]; ppublish Place of Publication Netherlands ISSN/ISBN 1874-1754; 0167-5273 Accession Number PMID: 25585363 Language eng SubFile Case Reports; Letter; IM DOI 10.1016/j.ijcard.2014.12.122 [doi] Output Language Unknown(0) PMID 25585363 Abstract Descriptors Links Book Title Database Publisher Data Source Authors Santoro,F., Pellegrino,P.L., D'Arienzo,G., Ziccardi,L., Di Biase,M., Brunetti,N.D. Original/Translated Title URL Date of Electronic 20141230 PMCID Editors
Pyrosequencing vs. culture-dependent approaches to analyze lactic acid bacteria associated to chicha, a traditional maize-based fermented beverage from Northwestern Argentina	2015	Departamento de Microbiologia y Ecologia, Universitat de Valencia (UVEG), Av. Dr. Moliner 50, 46100, Burjassot, Valencia, Spain.; Departamento de Microbiologia y Ecologia, Universitat de Valencia (UVEG), Av. Dr. Moliner 50, 46100, Burjassot, Valencia, Spa	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full International journal of food microbiology Periodical, Abbrev. Int.J.Food Microbiol. Pub Date Free Form 2-Apr Volume 198 Issue Start Page 9 Other Pages 18 Notes CI: Copyright (c) 2014; JID: 8412849; 0 (RNA, Ribosomal, 16S); OTO: NOTNLM; 2014/07/23 [received]; 2014/12/09 [revised]; 2014/12/21 [accepted]; 2014/12/27 [aheadofprint]; ppublish Place of Publication Netherlands ISSN/ISBN 1879-3460; 0168-1605 Accession Number PMID: 25584777 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1016/j.ijfoodmicro.2014.12.027 [doi] Output Language Unknown(0) PMID 25584777 Abstract The diversity of lactic acid bacteria (LAB) associated with chicha, a traditional maize-based fermented alcoholic beverage from Northwestern Argentina, was analyzed using culture-dependent and culture-independent approaches. Samples corresponding to 10 production steps were obtained from two local producers at Maimara (chicha M) and Tumbaya (chicha T). Whereas by culture-dependent approach a few number of species (Lactobacillus plantarum and Weissella viridescens in chicha M, and Enterococcus faecium and Leuconostoc mesenteroides in chicha T) were identified, a higher quantitative distribution of taxa was found in both beverages by pyrosequencing. The relative abundance of OTUs was higher in chicha M than in chicha T; six LAB genera were common for chicha M and T: Enterococcus, Lactococcus, Streptococcus, Weissella, Leuconostoc and Lactobacillus while Pediococcus only was detected in chicha M. Among the 46 identified LAB species, those of Lactobacillus were dominant in both chicha samples, exhibiting the highest diversity, whereas Enterococcus and Leuconostoc were recorded as the second dominant genera in chicha T and M, respectively. Identification at species level showed the predominance of Lb. plantarum, Lactobacillus rossiae, Leuconostoc lactis and W. viridescens in chicha M while Enterococcus hirae, E. faecium, Lc. mesenteroides and Weissella confusa predominated in chicha T samples. In parallel, when presumptive LAB isolates (chicha M: 146; chicha T: 246) recovered from the same samples were identified by ISR-PCR and RAPD-PCR profiles, species-specific PCR and 16S rRNA gene sequencing, most of them were assigned to the Leuconostoc genus (Lc. mesenteroides and Lc. lactis) in chicha M, Lactobacillus, Weissella and Enterococcus being also present. In contrast, chicha T exhibited the presence of Enterococcus and Leuconostoc, E. faecium being the most representative species. Massive sequencing approach was applied for the first time to study the diversity and evolution of microbial communities during chicha manufacture. Although differences in the LAB species profile between the two geographically different chicha productions were observed by culturing, a larger number for predominant LAB species as well as other minorities were revealed by pyrosequencing. The fine molecular inventory achieved by pyrosequencing provided more precise information on LAB population composition than culture-dependent analysis processes. Descriptors Links Book Title Database Publisher Elsevier B.V Data Source Authors Elizaquivel,P., Perez-Cataluna,A., Yepez,A., Aristimuno,C., Jimenez,E., Cocconcelli,P.S., Vignolo,G., Aznar,R. Original/Translated Title URL Date of Electronic 20141227 PMCID Editors

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