Image
WTS Database
The subject of interest is:
waterpipe, water-pipe, nargile, narghile, arguileh, arguile, shisha, sheesha, chichi, hubble bubble, hubbly bubbly, goza, hookah, bong
| Title Sort descending | Pub Year | Author | SearchLink |
|---|---|---|---|
| Awareness, perceptions and use of snus among young adults from the upper Midwest region of the USA | 2013 | Division of Epidemiology and Community Health, University of Minnesota, Minneapolis, Minnesota, USA. | Read more... |
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Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Tobacco control
Periodical, Abbrev.
Tob.Control
Pub Date Free Form
Nov
Volume
22
Issue
6
Start Page
412
Other Pages
417
Notes
LR: 20150224; GR: R01 CA086191/CA/NCI NIH HHS/United States; GR: R01 CA86191/CA/NCI NIH HHS/United States; JID: 9209612; NIHMS582155; OID: NLM: NIHMS582155; OID: NLM: PMC4047643; OTO: NOTNLM; 2012/07/20 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
1468-3318; 0964-4563
Accession Number
PMID: 22821750
Language
eng
SubFile
Journal Article; Research Support, N.I.H., Extramural; IM
DOI
10.1136/tobaccocontrol-2011-050383 [doi]
Output Language
Unknown(0)
PMID
22821750
Abstract
BACKGROUND: Since its introduction in 2006, snus has been aggressively marketed by tobacco companies. However, little is known about the awareness, perceptions and use of snus among young adults after Camel and Marlboro snus were sold nationwide in 2010. METHODS: Data were collected from 2607 young adults (ages 20-28) who participated in the Minnesota Adolescent Community Cohort Study in 2010-2011. Data include awareness of snus, ever and past 30-day use, perceived potential of snus as a quit aid, and perceived harmfulness and addictiveness of snus relative to cigarettes. The authors assessed the associations between these outcome variables and socio-demographic characteristics. RESULTS: Overall, 64.8% of participants were aware of snus, 14.5% ever used snus and 3.2% used snus in the past 30 days. Men and participants who smoked >100 cigarettes in their lifetime were associated with these three outcomes (p
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Choi,K., Forster,J.
Original/Translated Title
URL
Date of Electronic
20120720
PMCID
PMC4047643
Editors
|
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| Bacaba beverage produced by Umutina Brazilian Amerindians: Microbiological and chemical characterization | 2015 | Departamento de Ciencia de Alimentos, Universidade Federal de Lavras, Lavras, MG, Brazil.; Departamento de Biologia, Universidade Federal de Lavras, Lavras, MG, Brazil.; Departamento de Biologia, Universidade Federal de Lavras, Lavras, MG, Brazil. | Read more... |
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Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Periodical, Abbrev.
Braz J.Microbiol.
Pub Date Free Form
Oct-Dec
Volume
46
Issue
4
Start Page
1207
Other Pages
1216
Notes
LR: 20160115; JID: 101095924; OID: NLM: PMC4704630; 2014/11/21 [received]; 2015/03/30 [accepted]; 2015/10/27 [aheadofprint]; ppublish
Place of Publication
Brazil
ISSN/ISBN
1678-4405; 1517-8382
Accession Number
PMID: 26691483
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1590/S1517-838246420140964 [doi]
Output Language
Unknown(0)
PMID
26691483
Abstract
Bacaba chicha is a beverage prepared by the indigenous Umutina people from the bacaba fruit (Oenocarpus bacaba), a purple berry that is rich in fat and carbohydrates, as well as a source of phenolic compounds. In this study, samples of bacaba chicha beverage were collected, and the microbial community was assessed using culture-dependent and -independent techniques. The nutritional composition and metabolite profiles were analyzed, and species belonging to lactic acid bacteria (LAB) and yeasts were detected. The LAB group detected by culture-dependent analysis included Enterococcus hormaechei and Leuconostoc lactis. Polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) detected additional Propionibacterium avidum, Acetobacter spp., and uncultured bacteria. Pichia caribbica and Pichia guilliermondii were detected in a culture-dependent method, and Pichia caribbica was confirmed by PCR-DGGE analysis. The pH value of the beverage was 6.2. The nutritional composition was as follows: 16.47 +/- 0.73 g 100 mL-1 dry matter, 2.2 +/- 0.0 g 100 mL-1 fat, 3.36 +/- 0.44 g 100 mL-1 protein, and 10.87 +/- 0.26 g 100 mL-1 carbohydrate. The metabolites detected were 2.69 g L-1 succinic acid, 0.9 g L-1 acetic acid, 0.49 g L-1 citric acid, 0.52 g L-1 ethanol, and 0.4 g L-1 glycerol. This is the first study to identify microbial diversity in bacaba chicha spontaneous fermentation. This study is also the starting step in the immaterial record of this Brazilian indigenous beverage prepared from bacaba fruit.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Puerari,C., Magalhaes-Guedes,K.T., Schwan,R.F.
Original/Translated Title
URL
Date of Electronic
20151027
PMCID
PMC4704630
Editors
|
|||
| Back pain associated factors in the first and the second national health surveys in Iran | 2009 | Read more... | |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Payesh - Health Monitor
Periodical, Abbrev.
Pub Date Free Form
Volume
8
Issue
3
Start Page
245
Other Pages
251
Notes
ID: 102442
Place of Publication
ISSN/ISBN
Accession Number
Language
englishPersian
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
To determine Back Pain BKP] associated factors in the First and the Second National Health Surveys in Iran 1st NHS and 2nd NHS]. This study is based on the information obtained from the 1st NHS conducted in the year 1991 and the 2nd NHS conducted in the year 2000 .27752 and 36945 subjects aged 15 and over were interviewed in the 1st NHS and the 2nd NHS respectively, of whom 24589 subjects from the 1st NHS and 36488 subjects from 2nd NHS were included in this study. BKP was considered as dependent variable and number of the surveys 1st NHS, 2nd NHS], residential area, age gender, education, marital status, smoking cigarette, smoking hubble bubble, spinal fractures, mental health, skeletal deformation and Body Mass Index BMI] were considered as independent variables. Logistic regression models have been applied for data analysis, using SPSS soft ware. It is indicated that odds of BKP in the 2nd NHS is less than odds of BKP in the 1st NHS. Odds of BKP increases with increase in age and BMI and it decreases with increase in level of education. Odds of BKP is higher in women and married subjects. Smoking, mental disorder, spinal fracture and skeletal deformation increase the odds of BKP. The findings showed that higher level of education, lower BMI and quitting smoking can help controlling BKP
Descriptors
Back Pain, Risk Factors, Health Surveys, Body Mass Index, Age Factors, Sex Factors, Marital Status, Smoking, Educational Status
Links
http://pesquisa.bvsalud.org/ghl/resource/en/emro-102442
Book Title
Database
GHL; http://www.globalhealthlibrary.net/
Publisher
Data Source
Authors
Saiepour,Nargess, Zeraatti,Hojjat, Abhari,Roya, Mohammad,Kazem
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
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| Background atmospheric levels of aldehydes, BTEX and PM10 pollutants in a medium-sized city of southern Italy | 2007 | Dipartimento di Scienze Ambientali, Seconda Universita di Napoli, Via Vivaldi 43, 81100 Caserta, Italy. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Annali di Chimica
Periodical, Abbrev.
Ann.Chim.
Pub Date Free Form
Aug
Volume
97
Issue
8
Start Page
597
Other Pages
604
Notes
LR: 20131121; JID: 7610375; 0 (Air Pollutants); 0 (Aldehydes); 0 (Benzene Derivatives); 0 (Particulate Matter); 0 (Vehicle Emissions); 0 (Xylenes); 3FPU23BG52 (Toluene); J64922108F (Benzene); L5I45M5G0O (ethylbenzene); ppublish
Place of Publication
Italy
ISSN/ISBN
0003-4592; 0003-4592
Accession Number
PMID: 17899874
Language
eng
SubFile
Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
17899874
Abstract
Background atmospheric levels of aldehydes, BTEX and PM10 pollutants were measured in the suburb of Caserta (Italy), 75 thousands inhabitants, 41degrees 04' N, on rainless weekdays and weekends during 2005. On weekdays the average daily concentrations (microg m(-3)) were 41.6 PM10, 8.6 benzene, 25.2 toluene, 6.3 ethylbenzene, 14.0 (m+p)-xylene, 11.7 o-xylene, 6.5 formaldehyde, 3.3 acetaldehyde. All the pollutant concentrations were strictly correlated (mean correlation coefficients = 0.90). At weekends the concentrations were lower by about 1.6 times. Both on weekdays and at weekends the PM10 and benzene levels exceeded the limits set by the EU Directive 30/1999 and 69/2000, respectively.
Descriptors
Air Pollutants/analysis, Aldehydes/analysis, Benzene/analysis, Benzene Derivatives/analysis, Environmental Monitoring, Italy, Particulate Matter/analysis, Seasons, Time Factors, Toluene/analysis, Vehicle Emissions, Xylenes/analysis
Links
Book Title
Database
Publisher
Data Source
Authors
Iovino,P., Salvestrini,S., Capasso,S.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
|
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| Bacterial biodiversity of traditional Zabady fermented milk | 2008 | Department of Dairy Science and Technology, Faculty of Agriculture, Alexandria University, Aflaton street, El-Shatby, Alexandria, Egypt. elbaradeig@yahoo.fr | Read more... |
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Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International journal of food microbiology
Periodical, Abbrev.
Int.J.Food Microbiol.
Pub Date Free Form
10-Feb
Volume
121
Issue
3
Start Page
295
Other Pages
301
Notes
JID: 8412849; 0 (DNA, Bacterial); 2006/11/09 [received]; 2007/04/11 [revised]; 2007/11/06 [accepted]; 2007/11/17 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
0168-1605; 0168-1605
Accession Number
PMID: 18077039
Language
eng
SubFile
Journal Article; IM
DOI
S0168-1605(07)00587-9 [pii]
Output Language
Unknown(0)
PMID
18077039
Abstract
The aim of this work was to identify the bacterial biodiversity of traditional Zabady fermented milk using PCR-temporal temperature gel electrophoresis (PCR-TTGE) and PCR-denaturing gradient gel electrophoresis (PCR-DGGE). Most of the identified bacterial species in Zabady samples belonged to lactic acid bacteria (LAB), e.g., Streptococcus thermophilus, Lactococcus garvieae, Lactococcus raffinolactis, Lactococcus lactis, Leuconostoc citreum, Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus johnsonii. Using the culture-dependent and independent methods, the streptococcal and lactococcal groups appeared to be the major bacterial species in Zabady fermented milk, whereas the lactobacilli were the minor ones. The main dominant species was St. thermophilus followed by Lc. garvieae. Other molecular tools, e.g., species-specific PCR assay and cloning and sequencing strategy were used to confirm the TTGE and DGGE results. Lc. garvieae, Lc. raffinolactis, Ln. citreum, and Lb. johnsonii were identified for the first time in this type of Egyptian fermented milk.
Descriptors
Biodiversity, Colony Count, Microbial, Cultured Milk Products/microbiology, DNA, Bacterial/analysis, Egypt, Electrophoresis, Gel, Pulsed-Field/methods, Food Microbiology, Lactobacillus/classification/isolation & purification, Lactococcus/classification/isolation & purification, Leuconostoc/classification/isolation & purification, Phylogeny, Polymerase Chain Reaction/methods, Species Specificity, Streptococcus/classification/isolation & purification
Links
Book Title
Database
Publisher
Data Source
Authors
El-Baradei,G., Delacroix-Buchet,A., Ogier,J. C.
Original/Translated Title
URL
Date of Electronic
20071117
PMCID
Editors
|
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| Bacterial community structure in kimchi, a Korean fermented vegetable food, as revealed by 16S rRNA gene analysis | 2005 | School of Biological Sciences, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International journal of food microbiology
Periodical, Abbrev.
Int.J.Food Microbiol.
Pub Date Free Form
15-Aug
Volume
103
Issue
1
Start Page
91
Other Pages
96
Notes
LR: 20061115; JID: 8412849; 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); 2004/03/30 [received]; 2004/06/04 [revised]; 2004/11/25 [accepted]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
0168-1605; 0168-1605
Accession Number
PMID: 16084269
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
S0168-1605(05)00076-0 [pii]
Output Language
Unknown(0)
PMID
16084269
Abstract
Kimchi is a traditional Korean food fermented from a variety of vegetables. We elucidated the microbial community structure of five commercially produced kimchis made from Chinese cabbage by examining culture-independent 16S rRNA gene clone libraries. Most of the clones (347 out of 348) belonged to lactic acid bacteria and included several species of the genera Lactobacillus, Leuconostoc and Weissella. Weissella koreensis was found in all the samples and predominated in three of them (42.6-82%). Leuconostoc gelidum, Leuconostoc gasicomitatum and Lactobacillus sakei were common in the remaining kimchi clone libraries (>34%). The composition of bacterial phylotypes in kimchi varied between samples. Our approach revealed different community structures from those reported in previous culture-dependent studies based on phenotypic identification methods. The culture-independent method used here proved to be efficient and accurate and showed that the bacterial communities in kimchi differ from those in other fermented vegetable foods.
Descriptors
Base Sequence, Brassica/microbiology, Colony Count, Microbial, Fermentation, Korea, Lactobacillus/classification/isolation & purification, Leuconostoc/classification/isolation & purification, Phylogeny, RNA, Bacterial/analysis, RNA, Ribosomal, 16S/analysis, Species Specificity
Links
Book Title
Database
Publisher
Data Source
Authors
Kim,M., Chun,J.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
|
|||
| Bacterial Composition of the Human Upper Gastrointestinal Tract Microbiome Is Dynamic and Associated with Genomic Instability in a Barrett's Esophagus Cohort | 2015 | Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, Washington, United States of America; Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America.; Human Biology, Fred Hutchinson | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
PloS one
Periodical, Abbrev.
PLoS One
Pub Date Free Form
15-Jun
Volume
10
Issue
6
Start Page
e0129055
Other Pages
Notes
LR: 20160610; BioProject/PRJNA270661; GR: K05 CA124911/CA/NCI NIH HHS/United States; GR: K05 CA124911/CA/NCI NIH HHS/United States; GR: P01 CA 91955/CA/NCI NIH HHS/United States; GR: P01 CA091955/CA/NCI NIH HHS/United States; GR: P30 CA015704/CA/NCI NIH H
Place of Publication
United States
ISSN/ISBN
1932-6203; 1932-6203
Accession Number
PMID: 26076489
Language
eng
SubFile
Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; IM
DOI
10.1371/journal.pone.0129055 [doi]
Output Language
Unknown(0)
PMID
26076489
Abstract
BACKGROUND: The incidence of esophageal adenocarcinoma (EAC) has increased nearly five-fold over the last four decades in the United States. Barrett's esophagus, the replacement of the normal squamous epithelial lining with a mucus-secreting columnar epithelium, is the only known precursor to EAC. Like other parts of the gastrointestinal (GI) tract, the esophagus hosts a variety of bacteria and comparisons among published studies suggest bacterial communities in the stomach and esophagus differ. Chronic infection with Helicobacter pylori in the stomach has been inversely associated with development of EAC, but the mechanisms underlying this association remain unclear. METHODOLOGY: The bacterial composition in the upper GI tract was characterized in a subset of participants (n=12) of the Seattle Barrett's Esophagus Research cohort using broad-range 16S PCR and pyrosequencing of biopsy and brush samples collected from squamous esophagus, Barrett's esophagus, stomach corpus and stomach antrum. Three of the individuals were sampled at two separate time points. Prevalence of H. pylori infection and subsequent development of aneuploidy (n=339) and EAC (n=433) was examined in a larger subset of this cohort. RESULTS/SIGNIFICANCE: Within individuals, bacterial communities of the stomach and esophagus showed overlapping community membership. Despite closer proximity, the stomach antrum and corpus communities were less similar than the antrum and esophageal samples. Re-sampling of study participants revealed similar upper GI community membership in two of three cases. In this Barrett's esophagus cohort, Streptococcus and Prevotella species dominate the upper GI and the ratio of these two species is associated with waist-to-hip ratio and hiatal hernia length, two known EAC risk factors in Barrett's esophagus. H. pylori-positive individuals had a significantly decreased incidence of aneuploidy and a non-significant trend toward lower incidence of EAC.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Gall,A., Fero,J., McCoy,C., Claywell,B.C., Sanchez,C.A., Blount,P.L., Li,X., Vaughan,T.L., Matsen,F.A., Reid,B.J., Salama,N.R.
Original/Translated Title
URL
Date of Electronic
20150615
PMCID
PMC4468150
Editors
|
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| Bacterial contamination of different components of the waterpipe | 2014 | Research Center for Social Determinants of Health, Institute for Futures Studies in Health, Kerman, Iran.; Research Center for Tropical and Infectious Diseases, Kerman, Iran.; Medical Student Research Committee, Kerman, Iran.; Neuroscience Research Center | Read more... |
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Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease
Periodical, Abbrev.
Int.J.Tuberc.Lung Dis.
Pub Date Free Form
Aug
Volume
18
Issue
8
Start Page
988
Other Pages
991
Notes
JID: 9706389; ppublish
Place of Publication
France
ISSN/ISBN
1815-7920; 1027-3719
Accession Number
PMID: 25199017
Language
eng
SubFile
Journal Article; IM
DOI
10.5588/ijtld.14.0026 [doi]
Output Language
Unknown(0)
PMID
25199017
Abstract
SETTING: Waterpipe smoking is an emerging topic in tobacco research that may have unrecognised health hazards. OBJECTIVE: To determine whether waterpipes are a source of bacterial contamination. METHODS: A total of 15 restaurants and waterpipe cafes were randomly selected from the list of locations serving waterpipe tobacco in Kerman city, Iran. Different parts of the waterpipe devices were sampled, including the disposable mouthpiece, the mouthpiece of the hose and the water in the bowl of the waterpipe. The samples were smeared onto bacterial culture media, including eosin methylene blue, blood agar and MacConkey agar growth media, and incubated at 37 degrees C. After 24-48 h, they were examined for colony growth. RESULTS: Of 285 samples from different parts of the waterpipes, 236 (82.8%) showed positive cultures; the rate of contamination ranged from 69% in the fixed mouthpiece to 96% in bowl water. Coagulase-negative staphylococci (32.9%), Streptococcus spp (26.9%), Neisseria spp (13.7%) and Escherichia coli (9.4%) were the most frequent contaminants. CONCLUSION: Waterpipes are frequently contaminated with microorganisms. This study revealed potential microbial hazards in waterpipes that may contribute to respiratory tract colonisation.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Safizadeh,H., Moradi,M., Rad,M.R., Nakhaee,N.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
|
|||
| Bacterial repopulation of drinking water pipe walls after chlorination | 2016 | a EPHE , PSL Research University, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, LCPME , UMR 7564 , Nancy , France.; b CNRS and Universite de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, LCPME , UM | Read more... |
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Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Biofouling
Periodical, Abbrev.
Biofouling
Pub Date Free Form
Sep
Volume
32
Issue
8
Start Page
925
Other Pages
934
Notes
JID: 9200331; OTO: NOTNLM; ppublish
Place of Publication
England
ISSN/ISBN
1029-2454; 0892-7014
Accession Number
PMID: 27483985
Language
eng
SubFile
Journal Article; IM
DOI
10.1080/08927014.2016.1212989 [doi]
Output Language
Unknown(0)
PMID
27483985
Abstract
The short-term kinetics of bacterial repopulation were evaluated after chlorination of high-density polyethylene (HDPE) colonized with drinking water biofilms and compared with bare HDPE surfaces. The effect of chlorination was partial as a residual biofilm persisted and was time-limited as repopulation occurred immediately after water resupply. The total number of bacteria reached the same levels on both the bare and chlorinated biofilm-fouled HDPE after a seven-day exposure to drinking water. Due to the presence of a residual biofilm, the hydrophobicity of chlorinated biofilm-fouled surface exhibited much lower adhesion forces (2.1 nN) compared to bare surfaces (8.9 nN). This could explain the rapid repopulation after chlorination, with a twofold faster bacterial accumulation rate on the bare HDPE surface. gamma-Proteobacteria dominated the early stages of repopulation of both surfaces and a shift in the dominance occurred over the colonization time. Such observations define a timescale for cleaning frequency in industrial environments and guidelines for a rinsing procedure using drinking water.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Mathieu,L., Francius,G., El Zein,R., Angel,E., Block,J.C.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
|
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| Bacterial species associated with traditional starter cultures used for fermented bamboo shoot production in Manipur state of India | 2010 | Microbial Resources Division, Institute of Bioresources and Sustainable Development, Takyelpat Institutional Area, Imphal-795001, Manipur, India. saccharomyces@rediffmail.com | Read more... |
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Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International journal of food microbiology
Periodical, Abbrev.
Int.J.Food Microbiol.
Pub Date Free Form
30-Sep
Volume
143
Issue
2-Jan
Start Page
1
Other Pages
8
Notes
CI: Copyright 2010; JID: 8412849; 0 (DNA, Bacterial); 0 (DNA, Intergenic); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); 0 (RNA, Ribosomal, 23S); 2010/01/23 [received]; 2010/06/16 [revised]; 2010/07/10 [accepted]; 2010/07/15 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
1879-3460; 0168-1605
Accession Number
PMID: 20696489
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.ijfoodmicro.2010.07.008 [doi]
Output Language
Unknown(0)
PMID
20696489
Abstract
Soidon is a non-salted acidic fermented food prepared from the succulent bamboo shoot tip of Schizostachyum capitatum Munro by using a traditional liquid starter called "soidon mahi" in Manipur state of India. In this study, 163 bacterial isolates associated with this starter samples were identified and their population distribution was investigated by amplified ribosomal DNA restriction analysis (ARDRA), 16S rDNA sequencing and randomly amplified polymorphic DNA (RAPD) analysis. This acidic starter (pH 4.5+/-0.15) was dominated by a characteristic association of Bacillus and lactic acid bacteria (LAB) together. The population distribution of dominant species were Bacillus subtilis 29.3%, Bacillus cereus 35.7%, Bacillus pumilus 2.6%, Lactobacillus brevis 9.6%, Lactobacillus plantarum 5.1%, Carnobacterium sp. 11.9%, Enterococcus faecium 1.2% and Pseudomonas fluorescens 4.6%. Alarming population load (10(6)-10(7)cfu/ml) of B. cereus in 87% of starter samples studied should raise concern regarding biosafety of soidon consumption. PCR amplification of 16S-23S rDNA intergenic transcribed spacer (ITS) region and ITS-RFLP profiles revealed a high diversity with eight subgroups in B. subtilis, five subgroups in B. cereus and three subgroups in L. brevis isolates. The most abundant B. subtilis subgroup IB.1 distributed in most of the samples showed very less clonal variability during RAPD analysis. The molecular methods used in this study identified the dominant strains of Bacillus and LAB distributed in most of the starter samples. These dominant strains of B. subtilis, L. brevis and L. plantarum would allow for developing a defined starter culture for the production of quality soidon.
Descriptors
Bacteria/classification/genetics/growth & development, Bacterial Load, Bacterial Typing Techniques/methods, Bambusa/metabolism/microbiology, DNA, Bacterial/analysis, DNA, Intergenic, DNA, Ribosomal, Fermentation, Food Handling, Food Microbiology, Food Safety, India, Plant Shoots, RNA, Ribosomal, 16S, RNA, Ribosomal, 23S, Random Amplified Polymorphic DNA Technique, Sequence Analysis, DNA
Links
Book Title
Database
Publisher
Elsevier B.V
Data Source
Authors
Jeyaram,K., Romi,W., Singh,T. A., Devi,A. R., Devi,S. S.
Original/Translated Title
URL
Date of Electronic
20100715
PMCID
Editors
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