WTS database

Title	Pub Year	Author Sort descending	SearchLink
Risk factors associated with Barrett's epithelial dysplasia	2014	Mikiko Fujita, Yuri Nakamura, Saeko Kasashima, Maiko Furukawa, Ryoichi Misaka, Hikaru Nagahara, Department of Gastroenterology, Aoyama Hospital, Tokyo Women's Medical University, Tokyo 1070061, Japan.; Mikiko Fujita, Yuri Nakamura, Saeko Kasashima, Maiko	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full World journal of gastroenterology Periodical, Abbrev. World J.Gastroenterol. Pub Date Free Form 21-Apr Volume 20 Issue 15 Start Page 4353 Other Pages 4361 Notes LR: 20151022; JID: 100883448; 0 (TP53 protein, human); 0 (Tumor Suppressor Protein p53); OID: NLM: PMC3989971; OTO: NOTNLM; 2013/09/05 [received]; 2013/10/27 [revised]; 2013/11/18 [accepted]; ppublish Place of Publication China ISSN/ISBN 2219-2840; 1007-9327 Accession Number PMID: 24764673 Language eng SubFile Journal Article; IM DOI 10.3748/wjg.v20.i15.4353 [doi] Output Language Unknown(0) PMID 24764673 Abstract AIM: To elucidate risk factors associated with dysplasia of short-segment Barrett's esophagus (BE). METHODS: A total of 151 BE patients who underwent endoscopic examination from 2004 to 2008 in Aoyama Hospital, Tokyo Women's Medical University, Japan and whose diagnosis was confirmed from biopsy specimens were enrolled in the study. BE was diagnosed based on endoscopic findings of gastric-appearing mucosa or apparent columnar-lined esophagus proximal to the esophagogastric junction. Dysplasia was classified into three grades - mild, moderate and severe - according to the guidelines of the Vienna Classification System for gastrointestinal epithelial neoplasia. Anthropometric and biochemical data were analyzed to identify risk factors for BE dysplasia. The prevalence of Helicobacter pylori (H. pylori) infection and the expression of p53 by immunohistological staining were also investigated. RESULTS: Histological examination classified patients into three types: specialized columnar epithelium (SCE) (n = 65); junctional (n = 38); and gastric fundic (n = 48). The incidence of dysplasia or adenocarcinoma from BE of the SCE type was significantly higher than that of the other two types (P Descriptors Links Book Title Database Publisher Data Source Authors Fujita,M., Nakamura,Y., Kasashima,S., Furukawa,M., Misaka,R., Nagahara,H. Original/Translated Title URL Date of Electronic PMCID PMC3989971 Editors
Species and susceptibility distribution of 1062 clinical yeast isolates to azoles, echinocandins, flucytosine and amphotericin B from a multi-centre study	2012	Mikrobiologische Beratung and Service, Munchen, Germany Division of Clinical Microbiology, Department of Laboratory Medicine, University Vienna, Austria.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Mycoses Periodical, Abbrev. Mycoses Pub Date Free Form May Volume 55 Issue 3 Start Page e124 Other Pages 37 Notes LR: 20131121; CI: (c) 2012; JID: 8805008; 0 (Antifungal Agents); 0 (Azoles); 0 (Echinocandins); 7XU7A7DROE (Amphotericin B); D83282DT06 (Flucytosine); 2012/01/11 [aheadofprint]; ppublish Place of Publication Germany ISSN/ISBN 1439-0507; 0933-7407 Accession Number PMID: 22233267 Language eng SubFile Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't; IM DOI 10.1111/j.1439-0507.2011.02165.x [doi] Output Language Unknown(0) PMID 22233267 Abstract Descriptive values were determined for eight antifungal agents within the course of a multi-centre study encompassing 1062 German and Austrian clinical yeast isolates. Candida albicans (54%) was the predominant species isolated followed by Candida glabrata (22%), Candida parapsilosis (6%), Candida tropicalis (5.7%), Candida krusei (4.3%), as well as eleven further candidal and four non-Candida yeast species. While 519 (48.9%) isolates were tested susceptible to all antifungals tested, no isolate was found to exhibit complete cross resistance. For C. albicans, the proportions of susceptible isolates were 93.2% (amphotericin B), 95.6% (flucytosine), 84.3% (fluconazole), 83.8% (posaconazole), 91.8% (voriconazole), 96.5% (anidulafungin), 96.2% (caspofungin) and 97.6% (micafungin). Patterns of complete parallel resistances were observed within azoles (8.8%) and echinocandins (1.7%). While a decreased susceptibility was found infrequently for echinocandins and flucytosine, it was more common for azoles with highest proportions for isolates of C. glabrata (fluconazole, 40.6%; posaconazole, 37.2%), Candida guilliermondii (fluconazole and posaconazole, each 25.0%), C. krusei (posaconazole, 28.3%; voriconazole, 60%), C. parapsilosis (fluconazole, 70.3%) and C. tropicalis (fluconazole, 62.3%). The descriptive values obtained in this study represent a valid basis for the comparison of recent and future epidemiological surveys to analyse the susceptibility of yeast isolates towards major antifungal substances. Descriptors Links Book Title Database Publisher Blackwell Verlag GmbH Data Source Authors Schmalreck,A.F., Willinger,B., Haase,G., Blum,G., Lass-Florl,C., Fegeler,W., Becker,K., Antifungal Susceptibility Testing-AFST Study Group Original/Translated Title URL Date of Electronic 20120111 PMCID Editors
Intraspecific variation of DNA per cell between Picea sitchensis (Bong.) Carr. provenances.	1971	Miksche, J.P.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Chromosoma Periodical, Abbrev. Chromosoma Pub Date Free Form / Volume 32 Issue 4 Start Page 343 Other Pages 352 Notes Place of Publication ISSN/ISBN 0009-5915 Accession Number Language SubFile DOI Output Language Unknown(0) PMID Abstract Descriptors DNA, analysis of variance, article, cell nucleus, cytology, densitometry, environment, metabolism, plant seed, spectrophotometry, tree, United States Links Book Title Database MEDLINE Publisher Data Source Embase Authors Miksche,J. P. Original/Translated Title URL Date of Electronic PMCID Editors
Preventive practices and non healthy behaviors among female university employees in Saudi Arabia	1999	Milaat, W.A., Department of Medicine, King AbdulAziz University, Jeddah, Egypt	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Bahrain Medical Bulletin Periodical, Abbrev. Bahrain Med.Bull. Pub Date Free Form 1999/ Volume 21 Issue 3 Start Page 75 Other Pages 79 Notes Place of Publication ISSN/ISBN 1012-8298 Accession Number Language SubFile DOI Output Language Unknown(0) PMID Abstract Objective: To assess the prevalence of non healthy behaviors and the practice of health preventive measures among female university employees in Jeddah, Saudi Arabia. Subjects and methods: Through a multi-stage stratified sample, a total of 299 female teaching staff and employees were interviewed by trained female medical students using a comprehensive questionnaire to determine non healthy behaviors and preventive measures practiced. Results: Educational level of this group was high and their jobs were mostly in teaching and administration. Only one fourth of them practiced any form of physical exercise weekly and over half of them were either obese or severely obese. Obesity was seen more among mothers and older age groups and physical exercise was practiced more by non Saudis. Cigarette and Shisha smoking were reported by 10% and 11% of the group, respectively. Consumption of fatty foods was prevalent in more than half of university employees, while seat belt use for 20 % of the time or more was very low (21.1%). Low rates of pap smear testing and mammography were reported and breast self examination (BSE) practice is still unpopular among Saudi females. BSE was highly associated with the performance of mammography and medical breast examination, while mammograms were carried out more in employees having relatives with breast cancer. Conclusion and recommendation: Results from this study supports the general picture of obesity, low physical activity and high fat intake among Saudis and emphasizes the need for promoting physical fitness among them. Low rates of preventive screening practices such as mammography and pap smear testing were reported and alarming rates of smoking among them were also documented. This all emphasizes the necessity for public educational efforts to promote healthy dietary habits and lifestyle and implement preventive screening tests. Descriptors academic achievement, adult, article, breast carcinoma, cancer screening, fat intake, female, female worker, fitness, health behavior, health promotion, human, lifestyle, major clinical study, mammography, nutrition, obesity, Papanicolaou test, prevalence, Saudi Arabia, smoking, university Links Book Title Database Embase Publisher Data Source Embase Authors Milaat,W. A., Al-Bar,H. S., Ghabrah,T. M., Abalkhail,B. A., Suliman,N. K. Original/Translated Title URL Date of Electronic PMCID Editors
Right-to-left shunt and risk of decompression illness with cochleovestibular and cerebral symptoms in divers: case control study in 101 consecutive dive accidents	2003	Military Teaching Hospital, Service de Reanimation, Toulon-Naval, France.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Critical Care Medicine Periodical, Abbrev. Crit.Care Med. Pub Date Free Form Jan Volume 31 Issue 1 Start Page 84 Other Pages 88 Notes LR: 20041117; JID: 0355501; CIN: Crit Care Med. 2003 Jul;31(7):2083. PMID: 12847414; CIN: Crit Care Med. 2004 Sep;32(9):1983; author reply 1983. PMID: 15343047; ppublish Place of Publication United States ISSN/ISBN 0090-3493; 0090-3493 Accession Number PMID: 12544998 Language eng SubFile Journal Article; AIM; IM DOI 10.1097/01.CCM.0000038040.42972.81 [doi] Output Language Unknown(0) PMID 12544998 Abstract OBJECTIVE: We investigated the role of right-to-left shunt with standardized transcranial Doppler ultrasonography in a large population of divers referred for symptoms of decompression illness. DESIGN: Case series compared with a control group. SETTING: Military teaching hospital, hyperbaric unit. PATIENTS: Patients were 101 consecutive divers with clinical evidence of decompression illness and a control group of 101 healthy divers. INTERVENTION: Specification of the type of decompression illness involved and detection/evaluation of right-to-left shunt by standardized transcranial Doppler. The degree of right-to-left shunt was defined as major if the number of high-intensity transient signals in the middle cerebral artery was >20. MEASUREMENTS AND MAIN RESULTS: We evaluated the odds ratios by logistic regression analysis with vs. without right-to-left shunt for subjects with cochleovestibular symptoms, cerebral decompression illness, spinal decompression illness, and Caisson sickness. Of the 101 divers presenting with decompression illness, transcranial Doppler detected a right-to-left shunt in 59 (58.4%), whereas control subjects demonstrated a right-to-left shunt in 25 cases (24.8%; odds ratio, 4.3; 95% confidence interval, 2.3-7.8; p=.09). When a right-to-left shunt was detected, the right-to-left shunt was major in 12 of 25 patients in the control group and in 49 of 59 patients in the decompression illness group (odds ratio, 8.7; 95% confidence interval, 4.2-18.0; p<.001). Within the decompression illness group, the proportion of major right-to-left shunt was 24 of 34 (odds ratio, 29.7; 95% confidence interval, 10.0-87.2; p<.0001) in the cochleovestibular subgroup, 13 of 21 (odds ratio, 24.1, 95% confidence interval, 6.8-86.0, p< 0.0001) in the cerebral decompression illness subgroup, ten of 31 (odds ratio, 3.9; 95% confidence interval, 1.5-10.3; p<.01) in the spinal decompression illness subgroup, and two of two (odds ratio, 1.1; 95% confidence interval, 0.2-5.7; p=.9) in the subgroup of divers with Caisson sickness. CONCLUSION: Based on our results, we conclude that major right-to-left shunt was associated with an increased incidence of cochleovestibular and cerebral decompression illness, suggesting paradoxical embolism as a potential mechanism. Descriptors Adult, Case-Control Studies, Cochlear Diseases/epidemiology/etiology, Decompression Sickness/epidemiology/etiology/ultrasonography, Diving/injuries, Embolism, Paradoxical/complications, Female, France/epidemiology, Heart Septal Defects, Atrial/complications/ultrasonography, Humans, Incidence, Logistic Models, Male, Risk, Ultrasonography, Doppler, Transcranial, Vestibular Diseases/epidemiology/etiology Links Book Title Database Publisher Data Source Authors Cantais,E., Louge,P., Suppini,A., Foster,P. P., Palmier,B. Original/Translated Title URL Date of Electronic PMCID Editors
Multiple Ebola virus haemorrhagic fever outbreaks in Gabon, from October 2001 to April 2002	2005	Ministere de la sante publique, Libreville, Gabon.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Bulletin de la Societe de pathologie exotique (1990) Periodical, Abbrev. Bull.Soc.Pathol.Exot. Pub Date Free Form Sep Volume 98 Issue 3 Start Page 224 Other Pages 229 Notes LR: 20061115; JID: 9212564; 0 (Antibodies, Viral); 0 (RNA, Viral); ppublish Place of Publication France ISSN/ISBN 0037-9085; 0037-9085 Accession Number PMID: 16267965 Language fre SubFile English Abstract; Journal Article; IM DOI Output Language Unknown(0) PMID 16267965 Abstract Outbreaks of Ebola virus haemorrhagic fever have been reported from 1994 to 1996 in the province of Ogooue Ivindo, a forest zone situated in the Northeast of Gabon. Each time, the great primates had been identified as the initial source of human infection. End of November 2001 a new alert came from this province, rapidly confirmed as a EVHV outbreak. The response was given by the Ministry of Health with the help of an international team under the aegis of WHO. An active monitoring system was implemented in the three districts hit by the epidemic (Zadie, Ivindo and Mpassa) to organize the detection of cases and their follow-up. A case definition has been set up, the suspected cases were isolated at hospital, at home or in lazarets and serological tests were performed. These tests consisted of the detection of antigen or specific IgG and the RT-PCR. A classification of cases was made according to the results of biological tests, clinical and epidemiological data. The contact subjects were kept watch over for 21 days. 65 cases were recorded among which 53 deaths. The first human case, a hunter died on the 28th of October 2001. The epidemic spreads over through family transmission and nosocomial contamination. Four distinct primary foci have been identified together with an isolated case situated in the South East of Gabon, 580 km away from the epicenter. Deaths happened within a delay of 6 days. The last death has been recorded on the 22nd of March 2002 and the end of the outbreak was declared on the 6th of May 2002. The epidemic spreads over the Gabon just next. Unexplained deaths of animals had been mentionned in the nearby forests as soon as August 2001: great primates and cephalophus. Samples taken from their carcasses confirmed a concomitant animal epidemic. Descriptors Animals, Antelopes/virology, Antibodies, Viral/blood/immunology, Cluster Analysis, Contact Tracing, Cross Infection/epidemiology/transmission, Disease Outbreaks, Disease Reservoirs, Ebolavirus/genetics/immunology/isolation & purification/pathogenicity, Follow-Up Studies, Food Microbiology, Gabon/epidemiology, Gorilla gorilla/virology, Haplorhini/virology, Hemorrhagic Fever, Ebola/diagnosis/epidemiology/mortality/prevention & control/transmission/veterinary, Humans, International Cooperation, Meat/virology, Patient Isolation, Porcupines/virology, Primate Diseases/epidemiology/transmission/virology, Primates/virology, Quarantine, RNA, Viral/blood, Retrospective Studies, Serologic Tests, World Health Organization Links Book Title Database Publisher Data Source Authors Nkoghe,D., Formenty,P., Leroy,E. M., Nnegue,S., Edou,S. Y., Ba,J. I., Allarangar,Y., Cabore,J., Bachy,C., Andraghetti,R., de Benoist,A. C., Galanis,E., Rose,A., Bausch,D., Reynolds,M., Rollin,P., Choueibou,C., Shongo,R., Gergonne,B., Kone,L. M., Yada,A., Roth,C., Mve,M. T. Original/Translated Title Plusieurs epidemies de fievre hemorragique due au virus Ebola au Gabon, d'octobre 2001 a avril 2002 URL Date of Electronic PMCID Editors
Current tobacco use and its associated factors among adults in a country with comprehensive ban on tobacco: findings from the nationally representative STEPS survey, Bhutan, 2014	2016	Ministry of Health, Thimphu, Bhutan.; Ministry of Health, Thimphu, Bhutan.; Ministry of Health, Thimphu, Bhutan.; Ministry of Health, Thimphu, Bhutan.; Ministry of Health, Thimphu, Bhutan.; Jawaharlal Institute of Postgraduate Medical Education and Resear	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Population health metrics Periodical, Abbrev. Popul.Health.Metr Pub Date Free Form 8-Aug Volume 14 Issue Start Page 28 Other Pages 016-0098-9. eCollection 2016 Notes LR: 20160814; JID: 101178411; OID: NLM: PMC4977656; OTO: NOTNLM; 2016 [ecollection]; 2016/01/23 [received]; 2016/06/20 [accepted]; 2016/08/08 [epublish]; epublish Place of Publication England ISSN/ISBN 1478-7954; 1478-7954 Accession Number PMID: 27507928 Language eng SubFile Journal Article; IM DOI 10.1186/s12963-016-0098-9 [doi] Output Language Unknown(0) PMID 27507928 Abstract BACKGROUND: Despite a comprehensive ban on cultivation, manufacture, distribution, and sale of tobacco products since 2004, two nationwide surveys conducted in 2012 and 2013 reported high tobacco use in Bhutan. National Health Survey 2012 reported that 4 % of the population aged 15-75 years used smoked tobacco and about 48 % used smokeless tobacco. Similarly, Global Youth Tobacco Survey (GYTS) of Bhutan reported tobacco use prevalence of 30.3 % in 2013. However, factors associated with this high tobacco use were not systematically studied. Hence, we assessed the prevalence of tobacco use and its associated sociodemographic, behavioral, and environmental factors. METHODS: This cross-sectional analytical study used secondary data collected in a nationally representative Non-communicable Disease Risk Factors Surveillance STEPS Survey 2014 conducted among Bhutanese adults (18-69 years). The survey included a total of 2820 adults; selected using multistage stratified cluster sampling. Weighted analysis was done to calculate the prevalence of tobacco use. Unadjusted and adjusted prevalence ratios were calculated using log binomial regression. RESULTS: The prevalence of current overall tobacco use was 24.8 % (95 % CI: 21.4-28.3) and that of smoked, smokeless, and dual forms (smoked and smokeless forms) were 7.4 % (95 % CI: 5.8-9.0), 19.7 % (95 % CI: 16.5-22.9), and 2.3 % (95 % CI: 1.8-2.9), respectively. Significantly higher prevalence of tobacco use in all forms was found among males, younger age groups, and alcohol users. The prevalence of smoked form was higher in urban areas compared to rural areas (11 % vs 6 %; aPR 1.8, 95 % CI: 1.5-2.0). Among individuals who reported having a non-communicable disease, the prevalence of smoked tobacco use was significantly lower than those who did not have disease (3.5 % vs. 8.3 %; aPR 0.5, 95 % CI: 0.3-0.9). Exposure to health warnings was protective for current tobacco use and smokeless tobacco use, while exposure to tobacco warnings through the media was helpful among smokers and overall tobacco users. CONCLUSIONS: Despite a comprehensive ban on tobacco, tobacco use was high in Bhutan, especially the smokeless form. Males, younger age groups, and alcohol users should be targeted with behavioral interventions along the stricter implementation of tobacco control measures. Descriptors Links Book Title Database Publisher Data Source Authors Gurung,M.S., Pelzom,D., Dorji,T., Drukpa,W., Wangdi,C., Chinnakali,P., Goel,S. Original/Translated Title URL Date of Electronic 20160808 PMCID PMC4977656 Editors
Electricity and water desalination: Separate sites offer value	1996	Moch Jr., I., DuPont Co., Wilmington, DE, United States	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Water, air, and soil pollution Periodical, Abbrev. Water Air Soil Pollut. Pub Date Free Form 1996/07 Volume 90 Issue 2-Jan Start Page 231 Other Pages 241 Notes Place of Publication ISSN/ISBN 0049-6979 Accession Number Language SubFile DOI Output Language Unknown(0) PMID Abstract For the fuel type and water situation in the Middle East, the case is strong for the use of combined cycle technology for power generation and reverse osmosis for potable water production, where each are sited for their maximum economic benefit and interconnected by electric power transmission. Because of the fuel efficiency of Combined Cycle generation technology, its use of liquid/gas fuels and its low need for cooling water, it can be optimized for cost away from cities. Conversely, water desalination by reverse osmosis can be sited in optimal locations to take advantage of its modularity and to minimize water pipeline needs. Electric power transmission provides an inexpensive and flexible means to connect these two technologies. Together these technologies may offer an overall minimum cost approach, better than the combining of electric power and water desalination at one location, where power to water ratios must be fixed, independent of need, for optimum efficiency. The use of reverse osmosis with power generation has other, important ancillary benefits over using distillation and power combinations. These advantages include abatement of environmental pollution, delivery of potable water at reasonable drinking temperatures, lower total energy consumption, more efficient land use and less demanding operator skills. Descriptors cooling water, cost benefit analysis, desalination, distillation, economic aspect, electric power plant, electricity, Middle East, reverse osmosis, review, water supply, water treatment Links Book Title Database Embase Publisher Data Source Embase Authors Moch Jr.,Dr I., Depenbrock,F., Mussalli,Y. Original/Translated Title URL Date of Electronic PMCID Editors
Determination of hydroxylated metabolites of polycyclic aromatic hydrocarbons in sediment samples by combining subcritical water extraction and dispersive liquid-liquid microextraction with derivatization	2012	MOE Key Laboratory of Aquatic Product Safety, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Analytica Chimica Acta Periodical, Abbrev. Anal.Chim.Acta Pub Date Free Form 13-Nov Volume 753 Issue Start Page 57 Other Pages 63 Notes LR: 20131121; CI: Copyright (c) 2012; JID: 0370534; 0 (Acetamides); 0 (Acetonitriles); 0 (Fluoroacetates); 0 (Organosilicon Compounds); 0 (Polycyclic Hydrocarbons, Aromatic); 0 (Water Pollutants, Chemical); 059QF0KO0R (Water); 77377-52-7 (N-methyl-N-(tert Place of Publication Netherlands ISSN/ISBN 1873-4324; 0003-2670 Accession Number PMID: 23107137 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1016/j.aca.2012.09.028 [doi] Output Language Unknown(0) PMID 23107137 Abstract A sample preparation method for the determination of hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) in sediment samples was developed using gas chromatography-mass spectrometry (GC-MS). Dispersive liquid-liquid microextraction (DLLME) with derivatization was performed following the subcritical water extraction (SWE) that provided which was provided by accelerated solvent extraction (ASE). Several important parameters that affected both SWE extraction and DLLME, such as the selection of organic modifier, its volume, extraction temperature, extraction pressure and extraction time were also investigated. High sensitivity of the hydroxylated PAHs derivatives by N-(tert-butyldimethylsilyl)-N-methyl-trifluoroacetamide (MTBSTFA) could be achieved with the limits of detection (LODs) ranging from 0.0139 (2-OH-nap) to 0.2334 mug kg(-1) (3-OH-fluo) and the relative standard deviations (RSDs) between 2.81% (2-OH-phe) and 11.07% (1-OH-pyr). Moreover, the proposed method was compared with SWE coupled with solid phase extraction (SPE), and the results showed that ASE-DLLME was more promising with recoveries ranging from 57.63% to 91.07%. The proposed method was then applied to determine the hydroxylated metabolites of phenanthrene in contaminated sediments produced during the degradation by two PAH-degraders isolated from mangrove sediments. Descriptors Links Book Title Database Publisher Elsevier B.V Data Source Authors Wang,X., Lin,L., Luan,T., Yang,L., Tam,N.F. Original/Translated Title URL Date of Electronic 20121001 PMCID Editors
Bacterial Composition of the Human Upper Gastrointestinal Tract Microbiome Is Dynamic and Associated with Genomic Instability in a Barrett's Esophagus Cohort	2015	Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, Washington, United States of America; Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America.; Human Biology, Fred Hutchinson	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full PloS one Periodical, Abbrev. PLoS One Pub Date Free Form 15-Jun Volume 10 Issue 6 Start Page e0129055 Other Pages Notes LR: 20160610; BioProject/PRJNA270661; GR: K05 CA124911/CA/NCI NIH HHS/United States; GR: K05 CA124911/CA/NCI NIH HHS/United States; GR: P01 CA 91955/CA/NCI NIH HHS/United States; GR: P01 CA091955/CA/NCI NIH HHS/United States; GR: P30 CA015704/CA/NCI NIH H Place of Publication United States ISSN/ISBN 1932-6203; 1932-6203 Accession Number PMID: 26076489 Language eng SubFile Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; IM DOI 10.1371/journal.pone.0129055 [doi] Output Language Unknown(0) PMID 26076489 Abstract BACKGROUND: The incidence of esophageal adenocarcinoma (EAC) has increased nearly five-fold over the last four decades in the United States. Barrett's esophagus, the replacement of the normal squamous epithelial lining with a mucus-secreting columnar epithelium, is the only known precursor to EAC. Like other parts of the gastrointestinal (GI) tract, the esophagus hosts a variety of bacteria and comparisons among published studies suggest bacterial communities in the stomach and esophagus differ. Chronic infection with Helicobacter pylori in the stomach has been inversely associated with development of EAC, but the mechanisms underlying this association remain unclear. METHODOLOGY: The bacterial composition in the upper GI tract was characterized in a subset of participants (n=12) of the Seattle Barrett's Esophagus Research cohort using broad-range 16S PCR and pyrosequencing of biopsy and brush samples collected from squamous esophagus, Barrett's esophagus, stomach corpus and stomach antrum. Three of the individuals were sampled at two separate time points. Prevalence of H. pylori infection and subsequent development of aneuploidy (n=339) and EAC (n=433) was examined in a larger subset of this cohort. RESULTS/SIGNIFICANCE: Within individuals, bacterial communities of the stomach and esophagus showed overlapping community membership. Despite closer proximity, the stomach antrum and corpus communities were less similar than the antrum and esophageal samples. Re-sampling of study participants revealed similar upper GI community membership in two of three cases. In this Barrett's esophagus cohort, Streptococcus and Prevotella species dominate the upper GI and the ratio of these two species is associated with waist-to-hip ratio and hiatal hernia length, two known EAC risk factors in Barrett's esophagus. H. pylori-positive individuals had a significantly decreased incidence of aneuploidy and a non-significant trend toward lower incidence of EAC. Descriptors Links Book Title Database Publisher Data Source Authors Gall,A., Fero,J., McCoy,C., Claywell,B.C., Sanchez,C.A., Blount,P.L., Li,X., Vaughan,T.L., Matsen,F.A., Reid,B.J., Salama,N.R. Original/Translated Title URL Date of Electronic 20150615 PMCID PMC4468150 Editors

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