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Effect of low-intensity continuous training on lung function and cardiorespiratory fitness in both cigarette and hookah smokers 2015 Laboratory of Pharmacology, Sfax Medicine Faculty SMF, University of Sfax, Tunisia; Bizerte Sciences Faculty, Department of Biology, University of Carthage. Tunisia; Research Unit (EM2S), Higher Institute of Sport and Physical Education of Sfax, Universit
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
African health sciences
Periodical, Abbrev.
Afr.Health.Sci.
Pub Date Free Form
Dec
Volume
15
Issue
4
Start Page
1170
Other Pages
1181
Notes
LR: 20160311; JID: 101149451; OID: NLM: PMC4765424; OTO: NOTNLM; ppublish
Place of Publication
Uganda
ISSN/ISBN
1729-0503; 1680-6905
Accession Number
PMID: 26958018
Language
eng
SubFile
Journal Article; IM
DOI
10.4314/ahs.v15i4.16 [doi]
Output Language
Unknown(0)
PMID
26958018
Abstract
BACKGROUND: The decline in cardiorespiratory fitness and lung function was higher in smokers. Training method could mitigate some of the negative consequences of smoking among smokers unable or unwilling to quit. OBJECTIVE: To examine the effects of continuous training on lungs functional capability and cardiorespiratory fitness in smokers. METHODS: Fifteen cigarette smokers, 14 hookah smokers, and 14 nonsmokers were assigned to low-intensity continuous training (20-30 minutes of running at 40% of maximum oxygen uptake (O2max)). Lung function and cardiorespiratory fitness parameters were determined using respectively spirometer and treadmill maximal exercise test. RESULTS: Continuous training improved forced expiratory volume in one second (FEV1) and forced expiratory flow at 50% of FVC (FEF50 %) in all participants, smokers and nonsmokers (p
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Data Source
Authors
Koubaa,A., Triki,M., Trabelsi,H., Masmoudi,L., Zeghal,K.N., Sahnoun,Z., Hakim,A.
Original/Translated Title
URL
Date of Electronic
PMCID
PMC4765424
Editors
Methodological problems in the article comparing lung function profiles and aerobic capacity of adult cigarette and hookah smokers after 12 weeks intermittent training 2015 Laboratory of Physiology, Faculty of Medicine of Sousse, University of Sousse, Sousse, Tunisia.; Department of Physiology and Functional Exploration, Farhat HACHED University Hospital of Sousse, Sousse, Tunisia.; Research Laboratory N degrees LR14ES05: In
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
The Libyan journal of medicine
Periodical, Abbrev.
Libyan J.Med.
Pub Date Free Form
21-Apr
Volume
10
Issue
Start Page
27760
Other Pages
Notes
LR: 20151111; JID: 101299403; CON: Libyan J Med. 2015;10:26680. PMID: 25694204; CIN: Libyan J Med. 2015;10:27760. PMID: 25920102; OID: NLM: PMC4408317; 2015 [ecollection]; epublish
Place of Publication
Sweden
ISSN/ISBN
1819-6357; 1819-6357
Accession Number
PMID: 25906843
Language
eng
SubFile
Comment; Letter; IM
DOI
10.3402/ljm.v10.27760 [doi]
Output Language
Unknown(0)
PMID
25906843
Abstract
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Ben Saad,H.
Original/Translated Title
URL
Date of Electronic
20150421
PMCID
PMC4408317
Editors
The relationship between venue indoor air quality and urinary cotinine levels among semiopen-air cafe employees: what factors determine the level of exposure? 2011 Laboratory of Physiology, School of Medicine, University of Athens, Greece.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of aerosol medicine and pulmonary drug delivery
Periodical, Abbrev.
J.Aerosol Med.Pulm.Drug Deliv.
Pub Date Free Form
Feb
Volume
24
Issue
1
Start Page
35
Other Pages
41
Notes
LR: 20131121; JID: 101475057; 0 (Particulate Matter); 0 (Tobacco Smoke Pollution); K5161X06LL (Cotinine); 2010/10/20 [aheadofprint]; ppublish
Place of Publication
United States
ISSN/ISBN
1941-2703; 1941-2711
Accession Number
PMID: 20961168
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; T
DOI
10.1089/jamp.2010.0837 [doi]
Output Language
Unknown(0)
PMID
20961168
Abstract
BACKGROUND: Exposure to second-hand smoke (SHS) is increasingly recognized as an occupational hazard to workers in the service industry. In areas of the world with moderate climates, open windows and doors are assumed to provide a work environment with only marginally increased exposures to SHS. METHODS: We measured indoor fine particulate matter (PM2.5) in 50 semiopen air venues in Greece during the summer. Cotinine levels from a nonsmoking employee of each of these venues were measured from a postshift urine sample. RESULTS: In these semiopen-air venues, the mean level of indoor PM 2.5 levels were 113.5 +/- 72.3 mug/m(3). The mean postshift urinary cotinine levels of nonworking workers in these venues was 15 ng/mL. PM2.5 levels were strongly correlated with urinary cotinine concentrations (Spearman's r = 0.914). Linear regression analyses indicated that when taking into account the time of the measurement, the day of the week, for each 1 cigarette/100 m(3) the indoor PM2.5 concentrations increased by 26.6 mug/m(3) [95% confidence interval (CI): 7.6-45.7 mug/m(3), p = 0.007) and urinary cotinine levels of nonsmoking workers increased by 5.0 ng/mL (95% CI: 0.4 to 9.6, p = 0.034). CONCLUSIONS: In a sample of bars and restaurants with windows and doors open, indoor PM2.5 concentrations were elevated and increased proportionately to the density of smoking. Cotinine levels of nonsmoking employees increased with indoor PM2.5 concentrations, and also with the density of smoking. Open windows and doors do not protect workers from exposure to second-hand smoke.
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Database
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Data Source
Authors
Karabela,M., Vardavas,C.I., Tzatzarakis,M., Tsatsakis,A., Dockery,D., Connolly,G.N., Behrakis,P.
Original/Translated Title
URL
Date of Electronic
20101020
PMCID
Editors
Six-minute walk test in non-insulin-dependent diabetes mellitus patients living in Northwest Africa 2012 Laboratory of Physiology, University of Sousse, Sousse, Tunisia.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Diabetes, metabolic syndrome and obesity : targets and therapy
Periodical, Abbrev.
Diabetes Metab.Syndr.Obes.
Pub Date Free Form
Volume
5
Issue
Start Page
227
Other Pages
245
Notes
LR: 20130530; JID: 101515585; OID: NLM: PMC3422904; OTO: NOTNLM; 2012/08/07 [epublish]; ppublish
Place of Publication
New Zealand
ISSN/ISBN
1178-7007; 1178-7007
Accession Number
PMID: 22924002
Language
eng
SubFile
Journal Article
DOI
10.2147/DMSO.S28642 [doi]
Output Language
Unknown(0)
PMID
22924002
Abstract
INTRODUCTION: International recommendations of the exploration of non-insulin-dependent diabetes mellitus (NIDDM) are focused on deficiency and not incapacity. AIMS: (1) To estimate the incapacity of NIDDM patients through the 6-minute walk test (6MWT) data. (2) To determine their 6-minute walk distance (6MWD) influencing factors (3) To compare data of NIDDM patient group (PG; n = 100) with those of two control groups (CG): CG1 (n = 174, healthy nonobese and nonsmoker); CG2 (n = 55, obese nondiabetic free from comorbidities). POPULATION AND METHODS: The anthropometric, socioeconomic, clinical, metabolic, and 6MWT data of 100 NIDDM patients (45 females) were collected. RESULTS: Total sample means +/- standard deviation of age, weight, and height were 54 +/- 8 years, 81 +/- 14 kg, and 1.64 +/- 0.09 m. (1) Measured 6MWD (566 +/- 81 m) was significantly lower than the theoretical 6MWD (90% +/- 12%). The profile of the PG carrying the 6MWT, was as follows: 23% had an abnormal 6MWD; at the end of the 6MWT, 21% and 12% had, respectively, a low heart rate and a high dyspnea (>5/10), and 4% had desaturation during the walk. The estimated "cardiorespiratory and muscular chain" age (68 +/- 16 years) was significantly higher than the chronological age. (2) The factors that significantly influenced the 6MWD (r(2) = 0.58) are included in the following equation: 6MWD (m) = -73.94 x gender (0, male; 1, female) - 3.25 x age (years) + 7.33 x leisure activity score - 35.57 x obesity (0, no; 1, yes) + 32.86 x socioeconomic level (0, low; 1, high) - 27.67 x cigarette use (0, no; 1, yes) + 8.89 x resting oxyhemoglobin saturation - 105.48. (3) Compared to the CGs, the PG had a significantly (P
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Database
Publisher
Data Source
Authors
Latiri,I., Elbey,R., Hcini,K., Zaoui,A., Charfeddine,B., Maarouf,M.R., Tabka,Z., Zbidi,A., Ben Saad,H.
Original/Translated Title
URL
Date of Electronic
20120807
PMCID
PMC3422904
Editors
Measurement of BTEX (benzene, toluene, ethybenzene, and xylene) levels at urban and semirural areas of Algiers City using passive air samplers 2012 Laboratory of Sciences and Environmental Techniques, National Polytechnic School of Algiers, 10, Hacen Badi Ave. BP 182, El Harrach, 16200 Algiers, Algeria. y_kerchic@hotmail.com
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of the Air & Waste Management Association (1995)
Periodical, Abbrev.
J.Air Waste Manag.Assoc.
Pub Date Free Form
Dec
Volume
62
Issue
12
Start Page
1370
Other Pages
1379
Notes
LR: 20140731; JID: 9503111; 0 (Air Pollutants); 0 (Benzene Derivatives); 0 (Vehicle Emissions); 0 (Xylenes); 3FPU23BG52 (Toluene); J64922108F (Benzene); L5I45M5G0O (ethylbenzene); ppublish
Place of Publication
United States
ISSN/ISBN
1096-2247; 1096-2247
Accession Number
PMID: 23362756
Language
eng
SubFile
Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
23362756
Abstract
The study presents the levels of air pollution by aromatic organic compounds BTEX (benzene, toluene, ethylbenzene, o-, m-, and p-xylenes) in the city of Algiers. The sampling was carried out using Radiello passive sampler. Three sampling campaigns were carried out in roadside, tunnel, urban background, and semirural sites in Algiers. In order to determine the diurnal mean levels of air pollution by BTEX to which people are exposed, a modified passive sampler was used for the first time. In addition, monitoring of pollution inside vehicles was also made. In the spring of 2009, more than 27 samplings were carried out. In the background and road traffic sites the Radiello sampler was exposed for 7 days, whereas the time exposure was reduced to 1 day in the case of the vehicle as well as the tunnel. The results indicate that average benzene concentrations in the roadside and inside vehicle exceed largely the limit value of 5 microg m(-3) established by the European Community (EC). On the other hand, it has been noticed that the concentration levels of other BTEX are relatively high. Also, in order to identify the origin of emission sources, ratios and correlations between the BTEX species have been highlighted. This study shows that road traffic remains the main source of many local emission in Algiers. IMPLICATIONS: The vehicle fleet in Algeria is growing rapidly since the 1990s following economic growth and is responsible for the increasing air pollution in large cities. Because there are no data collection of BTEX carried out by national air quality network, all environmental and transportation policies are based on European emissions standards, but national emission standards are currently not in place. This work will contribute to the analysis of real emissions of BTEX in Algiers, for the development of management and for assessment of population exposure variation depending on the location in the city of Algiers.
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Book Title
Database
Publisher
Data Source
Authors
Kerchich,Y., Kerbachi,R.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Early invasive pulmonary aspergillosis in a leukemia patient linked to aspergillus contaminated marijuana smoking 2001 Lahav, M., Medicine E Sapir Medical Center, Meir Hospital, Tel-Aviv University, Kfar Saba, Israel
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Leukemia and Lymphoma
Periodical, Abbrev.
Leuk.Lymphoma
Pub Date Free Form
2001/
Volume
42
Issue
6
Start Page
1433
Other Pages
1437
Notes
Place of Publication
ISSN/ISBN
1042-8194
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
46-year-old patient with acute myeloid leukemia (AML) whose disease manifested as fever, chills and dry cough is reported here. Despite broad antibiotic coverage he remained acutely ill with spiking fever, shaking chills, and hypoxemia. His initial chest radiograph was normal but chest computed tomography (CT) scan disclosed bilateral focal infiltrates. Hypoxemia and severe thrombocytopenia precluded invasive diagnostic procedures. A thorough epidemiological investigation revealed that before becoming acutely ill the patient smoked daily tobacco mixed with marijuana from a "hookah bottle". While waiting for tobacco and "hookah water" cultures, we started antifungal therapy. Resolution of fever and hypoxemia ensued after 72 hours. Tobacco cultures yielded heavy growth of Aspergillus species. We suggest that habitual smoking of Aspergillus-infested tobacco and marijuana caused airway colonization with Aspergillus. Leukemia rendered the patient immunocompromised, and allowed Aspergillus to infest the lung parenchyma with early occurrence of invasive pulmonary aspergillosis. Physicians should be aware of this potentially lethal complication of "hookah" and marijuana smoking in immunocompromised hosts.
Descriptors
amphotericin B, antibiotic agent, antifungal agent, cytarabine, etoposide, idarubicin, acute disease, acute myeloblastic leukemia, adult, antibiotic therapy, article, Aspergillus, cannabis addiction, case report, chill, computer assisted tomography, coughing, diagnostic procedure, disease severity, drug megadose, fever, fungal contamination, fungus culture, human, hypoxemia, immune deficiency, lung aspergillosis, lung infiltrate, lung parenchyma, male, priority journal, respiratory tract infection, smoking, thorax radiography, thrombocytopenia, tobacco, treatment failure
Links
Book Title
Database
Embase; MEDLINE
Publisher
Data Source
Embase
Authors
Szyper-Kravitz,M., Lang,R., Manor,Y., Lahav,M.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Efficacy of resident training in smoking cessation: a randomized, controlled trial of a program based on application of behavioral theory and practice with standardized patients 2002 Lausanne University Outpatient Clinic and Institute of Social and Preventive Medicine, Lausanne, Switzerland. Jacques.Cornuz@chuv.hospvd.ch
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Annals of Internal Medicine
Periodical, Abbrev.
Ann.Intern.Med.
Pub Date Free Form
19-Mar
Volume
136
Issue
6
Start Page
429
Other Pages
437
Notes
LR: 20061115; JID: 0372351; ppublish
Place of Publication
United States
ISSN/ISBN
1539-3704; 0003-4819
Accession Number
PMID: 11900495
Language
eng
SubFile
Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't; AIM; IM
DOI
200203190-00006 [pii]
Output Language
Unknown(0)
PMID
11900495
Abstract
BACKGROUND: New educational programs must be developed to improve physicians' skills and effectiveness in counseling patients about smoking cessation. OBJECTIVE: To assess the efficacy of an educational program based on behavioral theory, active learning methods, and practice with standardized patients in helping patients abstain from smoking and changing physicians' counseling practices. DESIGN: Cluster randomized, controlled trial. SETTING: Two general internal medicine clinics in Switzerland. PARTICIPANTS: 35 residents and 251 consecutive smoking patients. INTERVENTION: A training program administered over two half-days, during which physicians learned to provide counseling that matched smokers' motivation to quit and practiced these skills with standardized patients acting as smokers at different stages of change. The control intervention was a didactic session on management of dyslipidemia. MEASUREMENTS: Self-reported abstinence from smoking at 1 year of follow-up, which was validated by exhaled carbon monoxide testing at one clinic; score of overall quality of counseling based on use of 14 counseling strategies; patient willingness to quit; and daily cigarette consumption. RESULTS: At 1 year of follow-up, abstinence from smoking was significantly higher in the intervention group than in the control group (13% vs. 5%; P = 0.005); this corresponded to a cluster-adjusted odds ratio of 2.8 (95% CI, 1.4 to 5.5). Residents who received the study training provided better counseling than did those who received the control training (mean score, 4.0 vs. 2.7; P = 0.002). Smokers' willingness to quit was also higher in the intervention group (94% vs. 80%; P = 0.007). A nonsignificant trend toward lower daily cigarette consumption in the intervention group was observed. CONCLUSION: A training program in smoking cessation administered to physicians that was based on behavioral theory and practice with standardized patients significantly increased the quality of physicians' counseling, smokers' motivation to quit, and rates of abstinence from smoking at 1 year.
Descriptors
Adolescent, Adult, Aged, Behavior Therapy, Clinical Competence, Counseling/methods, Double-Blind Method, Family Practice/education, Female, Follow-Up Studies, Humans, Hyperlipidemias/prevention & control, Internal Medicine/education, Internship and Residency, Male, Motivation, Smoking Cessation/methods/psychology
Links
Book Title
Database
Publisher
Data Source
Authors
Cornuz,J., Humair,J. P., Seematter,L., Stoianov,R., van Melle,G., Stalder,H., Pecoud,A.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Diving pattern of fishermen in the Pescadores. 1994 Lee, H.C., Department of Hyperbaric Medicine, Naval General Hospital, Kaohsiung, ROC.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc
Periodical, Abbrev.
Undersea Hyperb.Med.
Pub Date Free Form
/
Volume
21
Issue
2
Start Page
145
Other Pages
158
Notes
Place of Publication
ISSN/ISBN
1066-2936
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
Professional diving fishermen in the Pescadores Archipelago (119.30 degrees W, 23.30 degrees N) dive with a simple hookah system. Although they use modern equipment, such as wet suit, face mask, mouth piece with demand valve, spear gun, weight belt, and fins, their lack of knowledge of diving medicine is apparent. On the average, 180 cases of decompression sickness (DCS) per year were reported to occur in the Pescadores. We conducted studies in 1990 and 1992 on three islands of the Archipelago. At the time of our study, an estimated 140 diving fishermen resided on these three islands. Of the 62 fishermen interviewed, 14 volunteered for the recording of dive profiles using a diver-carried data logger. In the summer of 1990, a group of eight fishermen dived, on the average, to a depth of 17.8 +/- 5.3 m (mean +/- SD; range, 8-27 m) for 26.9 +/- 19.7 min (5-66 min). Although the diving depth was similar to that of the average recollections of 43 divers, 20.1 +/- 4.4 m (15-30 m), the actual diving time was far shorter than that of their recollections, 426 +/- 138 min (240-630 min). The post-typhoon sea floor conditions may have shortened their diving time. In the summer of 1992, a group of six fishermen dived to 20.5 +/- 3.8 m (15-26 m) for 56.4 +/- 21.2 min (18-84 min). Again, the diving depth matched that of their recollection well, 22.2 +/- 2.5 m (20-26 m), but their bottom time was far shorter than they believed, 270 +/- 108 min (120-480 min). They used no decompression procedures, regardless of the bottom time and diving depth. In the 1990 group, 5 out of 10 equivalent single dive bottom times (ESDBT) exceeded U.S. Navy no-decompression (No-D) air dive limits; whereas in the 1992 group, 7 out of 9 ESDBTs exceeded No-D limits. Eight of the 38 discrete dives exceeded the No-D limits, even if we underestimate their decompression stress by disregarding their repetitive dive history. However, no symptoms of DCS were observed in either the 1990 or 1992 groups of 14 divers, despite 63% of the ESDBTs and 21% of discrete dives having exceeded the No-D limits. Adaptation to diving work may have allowed them to exceed the established No-D limits. The existing records of incidence of DCS from this region suggest that previously they must have dived longer or deeper or both during times of abundant resources.
Descriptors
adult, article, diving, heart rate, human, Japan, middle aged, Pacific islands, physiology, statistics, time
Links
Book Title
Database
MEDLINE
Publisher
Data Source
Embase
Authors
Lee,H. C., Niu,K. C., Huang,K. L., Tsai,J. D., Shyu,R. K., Shiraki,K., Hong,S. K., Lin,Y. C.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Phenotypic and genotypic analyses of lactic acid bacteria in local fermented food, breast milk and faeces of mothers and their babies 2011 Lehrstuhl fur Technische Mikrobiologie, Technische Universitat Munchen, Weihenstephaner Steig 16, 85350 Freising, Germany.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Systematic and applied microbiology
Periodical, Abbrev.
Syst.Appl.Microbiol.
Pub Date Free Form
Apr
Volume
34
Issue
2
Start Page
148
Other Pages
155
Notes
CI: Copyright (c) 2011; JID: 8306133; 2010/11/05 [received]; 2010/12/20 [revised]; 2010/12/21 [accepted]; ppublish
Place of Publication
Germany
ISSN/ISBN
1618-0984; 0723-2020
Accession Number
PMID: 21300508
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.syapm.2010.12.001 [doi]
Output Language
Unknown(0)
PMID
21300508
Abstract
Lactic acid bacteria (LAB) are generally accepted as beneficial to the host and their presence is directly influenced by ingestion of fermented food or probiotics. While the intestinal lactic microbiota is well-described knowledge on its routes of inoculation and competitiveness towards selective pressure shaping the intestinal microbiota is limited. In this study, LAB were isolated from faecal samples of breast feeding mothers living in Syria, from faeces of their infants, from breast milk as well as from fermented food, typically consumed in Syria. A total of 700 isolates were characterized by genetic fingerprinting with random amplified polymorphic DNA (RAPD) and identified by comparative 16S rDNA sequencing and Matrix Assisted Laser Desorption Ionization-Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS) analyses. Thirty six different species of Lactobacillus, Enterococcus, Streptococcus, Weissella and Pediococcus were identified. RAPD and MALDI-TOF-MS patterns allowed comparison of the lactic microbiota on species and strain level. Whereas some species were unique for one source, Lactobacillus plantarum, Lactobacillus fermentum, Pediococcus pentosaceus and Lactobacillus brevis were found in all sources. Interestingly, identical RAPD genotypes of L. plantarum, L. fermentum, L. brevis, Enterococcus faecium, Enterococcus faecalis and P. pentosaceus were found in the faeces of mothers, her milk and in faeces of her babies. Diversity of RAPD types found in food versus human samples suggests the importance of host factors in colonization and individual host specificity, and support the hypothesis that there is a vertical transfer of intestinal LAB from the mother's gut to her milk and through the milk to the infant's gut.
Descriptors
Links
Book Title
Database
Publisher
Elsevier GmbH
Data Source
Authors
Albesharat,R., Ehrmann,M.A., Korakli,M., Yazaji,S., Vogel,R.F.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
The microbial diversity of water kefir 2011 Lehrstuhl fur Technische Mikrobiologie, Weihenstephaner Steig 16, 85350 Freising, Germany.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International journal of food microbiology
Periodical, Abbrev.
Int.J.Food Microbiol.
Pub Date Free Form
15-Dec
Volume
151
Issue
3
Start Page
284
Other Pages
288
Notes
CI: Copyright (c) 2011; JID: 8412849; 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); 2011/04/27 [received]; 2011/09/09 [revised]; 2011/09/18 [accepted]; 2011/09/24 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
1879-3460; 0168-1605
Accession Number
PMID: 22000549
Language
eng
SubFile
Journal Article; IM
DOI
10.1016/j.ijfoodmicro.2011.09.016 [doi]
Output Language
Unknown(0)
PMID
22000549
Abstract
The microbial diversity of water kefir, made from a mixture of water, dried figs, a slice of lemon and sucrose was studied. The microbial consortia residing in the granules of three water kefirs of different origins were analyzed. A collection of 453 bacterial isolates was obtained on different selective/differential media. Bacterial isolates were grouped with randomly amplified polymorphic DNA (RAPD)-PCR analyses. One representative of each RAPD genotype was identified by comparative 16S rDNA gene sequencing. The predominant genus in water kefirs I and II was Lactobacillus, which accounted for 82.1% in water kefir I and 72.1% in water kefir II of the bacterial isolates. The most abundant species in water kefirs I and II were Lactobacillus hordei and Lb. nagelii followed by considerably lower numbers of Lb. casei. Other lactic acid bacteria (LAB) were identified as Leuconostoc mesenteroides and Lc. citreum in all three water kefirs. The most abundant species in water kefir III was Lc. mesenteroides (28%) and Lc. citreum (24.3%). A total of 57 LAB belonging to the species of Lb. casei, Lb. hordei, Lb. nagelii, Lb. hilgardii and Lc. mesenteroides were able to produce exopolysacchrides from sucrose. Non LABs were identified as Acetobacter fabarum and Ac. orientalis. The Acetobacter species were more prevalent in consortium III. Cluster analyses of RAPD-PCR patterns revealed an interspecies diversity among the Lactobacillus and Acetobacter strains. Aditionally, Saccharomyces cerevisiae, Lachancea fermentati, Hanseniaospora valbyensis and Zygotorulaspora florentina were isolated and identified by comparison of partial 26S rDNA sequences and FTIR spectroscopy.
Descriptors
Links
Book Title
Database
Publisher
. Published by Elsevier B.V
Data Source
Authors
Gulitz,A., Stadie,J., Wenning,M., Ehrmann,M.A., Vogel,R.F.
Original/Translated Title
URL
Date of Electronic
20110924
PMCID
Editors