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Co-operation of private water management sector with municipalities 2002 Kyncl, M., Severomoravske vodovody, kanalizace Ostrava a.s., 709 45 Ostrava, Czech Republic
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Water Science and Technology: Water Supply
Periodical, Abbrev.
Water Sc.Technol.Water Supply
Pub Date Free Form
2002/
Volume
2
Issue
4
Start Page
219
Other Pages
224
Notes
Place of Publication
ISSN/ISBN
1606-9749
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
After 1990 a gradual privatisation of the water and sewerage sector began in the Czech Republic as well as in other Central and Eastern European countries. State assets of public water pipelines and sewerage were transferred to towns and municipalities and joint stock companies were established. Two types of companies appeared-operational and ownership companies. The first type is the operational company that operates the infrastructure assets of public water pipelines and sewerage. These assets stay in the ownership of municipalities. A long term operational contract is agreed that solves the relationship between the owners and operators. The second type is the ownership company that directly owns the infrastructure assets of public water pipelines. Towns and municipalities in this case became shareholders of those companies. At the end of nineties, big international companies bought shares of both operational and ownership companies. Municipalities have to, by law, ensure the operation of public water pipelines. However, by the sale of shares they have lost a possibility to participate in decision making. A question about future co-operation between towns and municipalities and ownership companies appears. An example of the North Moravian Water and Sewerage Works Company was used to describe a possible way of co-operation between a private company and the municipalities on whose territory the company operates. Contracts between the ownership company and particular municipalities were agreed. This solution turned out to be complicated, so the municipalities affiliated and established Water Management Association. The objective of the Association is to promote common interests of the municipalities in relation to the water company. A contract was agreed between the Water Management Association and the company about co-operation in the area of water tariff setting and public waterworks' development.
Descriptors
article, Czech Republic, decision making, Europe, government, organization, pipeline, water management
Links
Book Title
Database
Embase
Publisher
Data Source
Embase
Authors
Kyncl,M.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Epidemiology of bladder cancer in Alexandria, Egypt: Tobacco smoking 1997 La Vecchia, C., Ricerche Farmacol. Inst. 'M. Negri', 20157 Milan, Italy
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International Journal of Cancer
Periodical, Abbrev.
Int.J.Cancer
Pub Date Free Form
1997/
Volume
73
Issue
1
Start Page
64
Other Pages
67
Notes
Place of Publication
ISSN/ISBN
0020-7136
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
The relationship between smoking and bladder cancer risk was investigated using data from a case-control study conducted between January 1994 and July 1996 in Alexandria, Egypt. Cases were 151 males with incident, histologically confirmed invasive cancer of the bladder, and controls were 157 males admitted to hospital for acute, non-neoplastic, non-urinary tract, non-smoking-related conditions. With reference to never smokers, ex-smokers had a multivariate odds ratio (OR) of 4.4 [95% confidence interval (Cl) 1.7- [1.7] and current smokers of 6.6 (95% Cl 3.1-13.9). The ORs were 5.4 for 40 years), and inversely related to age at starting (OR of 8.8 for starting <20 years), and inversely related to time since quitting smoking. Compared with never smokers who did not report a clinical history of schistosomiasis, the OR was 9.4 for smokers with a history of schistosomiasis, and 10.7 for smokers ever employed in high-risk occupations compared with non-smokers not reporting such a history. Thus, our results, while not giving indications of an increased bladder cancer risk with habits other than cigarette smoking, found a remarkably strong association with various measures of cigarette smoking that could explain 75% of bladder cancer cases among males from Alexandria. The prevalence of smoking was very low among women, and consequently tobacco was not a relevant risk factor for female bladder cancer.
Descriptors
article, bladder cancer, cancer epidemiology, cancer risk, controlled study, Egypt, human, major clinical study, male, priority journal, risk factor, smoking
Links
Book Title
Database
Embase; MEDLINE
Publisher
Data Source
Embase
Authors
Bedwani,R., El-Khwsky,F., Renganathan,E., Braga,C., Abu Seif,H. H., Azm,T. A., Zaki,A., Franceschi,S., Boffetta,P., La Vecchia,C.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Role of discontinuous chlorination on microbial production by drinking water biofilms 2005 Lab. Health & Environmental Microbiology, Polytechnical University of Catalonia, Terrassa-08222, Barcelona, Spain.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Water research
Periodical, Abbrev.
Water Res.
Pub Date Free Form
May
Volume
39
Issue
9
Start Page
1896
Other Pages
1906
Notes
LR: 20131121; JID: 0105072; 4R7X1O2820 (Chlorine); 2003/11/19 [received]; 2004/09/16 [revised]; 2005/02/21 [accepted]; 2005/04/26 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
0043-1354; 0043-1354
Accession Number
PMID: 15899288
Language
eng
SubFile
Journal Article; IM
DOI
S0043-1354(05)00107-7 [pii]
Output Language
Unknown(0)
PMID
15899288
Abstract
Microbial quality in water distribution systems is strongly affected by the development of microbial biofilms. Production and release of microbial cells by the biofilm affect microbial levels in the water column and in some cases this fact constitutes a public health concern. In this study, we attempt to analyze in which way the existence of different episodes of chlorine depletion affects both biofilm formation and microbial load of an artificial laboratory system. The work was carried out using two parallel packed bed reactors both supplied with running tap water. One of the reactors was used as a control and was permanently exposed to the action of chlorine. In the other reactor, chlorine was neutralized at selected times during the experiment and for periods of variable length. During the experiment the concentration of total and viable cells from the effluent was monitored at the exit of each of the reactors. The data obtained were used to estimate microbial production from the biofilms. As an average, release of microbial cells to the water phase increased tenfold in the absence of chlorine. The results also indicate that disinfectant efficiency against the biofilm was not recovered when chlorine returned to normal levels after each event of chlorine neutralization. Cell viability in the water phase in the presence of chlorine was low at the beginning of the experiment but increased 4 orders of magnitude after five neutralization periods. Therefore, subsequent episodes of chlorine depletion may accelerate the development of microbial communities with reduced susceptibility to disinfection in real drinking water systems.
Descriptors
Biofilms/growth & development, Bioreactors, Chlorine/analysis/chemistry, Colony Count, Microbial, Water Microbiology, Water Purification/methods, Water Supply
Links
Book Title
Database
Publisher
Data Source
Authors
Codony,F., Morato,J., Mas,J.
Original/Translated Title
URL
Date of Electronic
20050426
PMCID
Editors
Pyrolysis of cannabinoids: A model experiment in the study of cannabis smoking 1978 Lab. Organ. Chem., State Univ., Utrecht
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Bulletin on narcotics
Periodical, Abbrev.
Bull.Narc.
Pub Date Free Form
1978/
Volume
30
Issue
3
Start Page
55
Other Pages
59
Notes
Place of Publication
ISSN/ISBN
0007-523X
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
Since cannabis products are generally administered by smoking, it is of importance to get a better understanding of the effects of the smoking process on cannabinoids. Several investigators have studied the conversions of cannabinoids during smoking. However, the information available remains limited and even contradictory. Several reasons can be indicated for this discrepancy. The pyrolytic products of the cannabinoids are masked by the bulk of the substrate which is smoked. On the other hand, a relative change in the composition of the cannabinoids after smoking does not necessarily implicate a mutual conversion, which is however, a conclusion generally observed in literature. Moreover, the experimental techniques used by different research groups are very diverse: except for the material which is to be smoked and which is subject to large variations (marijuana, hashish, cannabis extracts mixed with tobacco, which is often pre-treated), the smoking-techniques also differ to a great extent (e.g., cigarette, pipe, water-pipe). The only common feature of these techniques is that cannabis is subjected to a thermal treatment. This situation calls for additional, more fundamental insight into the processes taking place during the smoking of cannabinoids. It was hoped that comparison of the products formed by smoking cannabidiol (I, CBD) through a water-pipe which is the general way of smoking by heavy cannabis users in the eastern countries, and the products formed by treatment of CBD under several pyrolytic conditions may contribute to reach this goal.
Descriptors
cannabidiol, cannabinoid, cannabis, inhalational drug administration, nonbiological model, pharmacokinetics, pyrolysis, smoking, theoretical study
Links
Book Title
Database
Embase; MEDLINE
Publisher
Data Source
Embase
Authors
Spronck,H. J. W., Salemink,C. A., Alikaridis,F., Papadakis,D.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Determination of organochlorine pesticides in water samples by dispersive liquid-liquid microextraction coupled to gas chromatography-mass spectrometry 2009 Labaqua S.A., C/Dracma 16-18, Pol. Ind. Las Atalayas, 03114 Alicante, Spain.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Analytica Chimica Acta
Periodical, Abbrev.
Anal.Chim.Acta
Pub Date Free Form
7-Sep
Volume
649
Issue
2
Start Page
218
Other Pages
221
Notes
LR: 20131121; JID: 0370534; 0 (Hydrocarbons, Chlorinated); 0 (Pesticides); 0 (Water Pollutants, Chemical); 059QF0KO0R (Water); 2009/05/18 [received]; 2009/07/14 [revised]; 2009/07/15 [accepted]; 2009/07/21 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
1873-4324; 0003-2670
Accession Number
PMID: 19699397
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.aca.2009.07.041 [doi]
Output Language
Unknown(0)
PMID
19699397
Abstract
A rapid and simple dispersive liquid-liquid microextraction (DLLME) has been developed to preconcentrate eighteen organochlorine pesticides (OCPs) from water samples prior to analysis by gas chromatography-mass spectrometry (GC-MS). The studied variables were extraction solvent type and volume, disperser solvent type and volume, aqueous sample volume and temperature. The optimum experimental conditions of the proposed DLLME method were: a mixture of 10 microL tetrachloroethylene (extraction solvent) and 1 mL acetone (disperser solvent) exposed for 30 s to 10 mL of the aqueous sample at room temperature (20 degrees C). Centrifugation of cloudy solution was carried out at 2300 rpm for 3 min to allow phases separation. Finally, 2 microL of extractant was recovered and injected into the GC-MS instrument. Under the optimum conditions, the enrichment factors ranged between 46 and 316. The calculated calibration curves gave a high-level linearity for all target analytes with correlation coefficients ranging between 0.9967 and 0.9999. The repeatability of the proposed method, expressed as relative standard deviation, varied between 5% and 15% (n=8), and the detection limits were in the range of 1-25 ng L(-1). The LOD values obtained are able to detect these OCPs in aqueous matrices as required by EPA methods 525.2 and 625. Analysis of spiked real water samples revealed that the matrix had no effect on extraction for river, surface and tap waters; however, urban wastewater sample shown a little effect for five out of eighteen analytes.
Descriptors
Fresh Water/analysis/chemistry, Gas Chromatography-Mass Spectrometry/methods, Hydrocarbons, Chlorinated/analysis/chemistry/isolation & purification, Pesticides/analysis/isolation & purification, Water/chemistry, Water Pollutants, Chemical/analysis/chemistry/isolation & purification
Links
Book Title
Database
Publisher
Data Source
Authors
Cortada,C., Vidal,L., Pastor,R., Santiago,N., Canals,A.
Original/Translated Title
URL
Date of Electronic
20090721
PMCID
Editors
Determination of cyclic and linear siloxanes in wastewater samples by ultrasound-assisted dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometry 2014 Labaqua S.A., C/Dracma 16-18, Pol. Ind. Las Atalayas, 03114 Alicante, Spain.; Labaqua S.A., C/Dracma 16-18, Pol. Ind. Las Atalayas, 03114 Alicante, Spain.; Departamento de Quimica Analitica, Nutricion y Bromatologia e Instituto de Materiales, Universidad
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Talanta
Periodical, Abbrev.
Talanta
Pub Date Free Form
Mar
Volume
120
Issue
Start Page
191
Other Pages
197
Notes
CI: (c) 2013; JID: 2984816R; 0 (Siloxanes); 0 (Solvents); 0 (Waste Water); 0 (Water Pollutants, Chemical); OTO: NOTNLM; 2013/07/23 [received]; 2013/11/08 [revised]; 2013/11/14 [accepted]; 2013/12/02 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
1873-3573; 0039-9140
Accession Number
PMID: 24468359
Language
eng
SubFile
Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.talanta.2013.11.042 [doi]
Output Language
Unknown(0)
PMID
24468359
Abstract
A fast, simple and environmentally friendly ultrasound-assisted dispersive liquid-liquid microextraction (USA-DLLME) procedure has been developed to preconcentrate eight cyclic and linear siloxanes from wastewater samples prior to quantification by gas chromatography-mass spectrometry (GC-MS). A two-stage multivariate optimization approach has been developed employing a Plackett-Burman design for screening and selecting the significant factors involved in the USA-DLLME procedure, which was later optimized by means of a circumscribed central composite design. The optimum conditions were: extractant solvent volume, 13 microL; solvent type, chlorobenzene; sample volume, 13 mL; centrifugation speed, 2300 rpm; centrifugation time, 5 min; and sonication time, 2 min. Under the optimized experimental conditions the method gave levels of repeatability with coefficients of variation between 10 and 24% (n=7). Limits of detection were between 0.002 and 1.4 microg L(-1). Calculated calibration curves gave high levels of linearity with correlation coefficient values between 0.991 and 0.9997. Finally, the proposed method was applied for the analysis of wastewater samples. Relative recovery values ranged between 71 and 116% showing that the matrix had a negligible effect upon extraction. To our knowledge, this is the first time that combines LLME and GC-MS for the analysis of methylsiloxanes in wastewater samples.
Descriptors
Links
Book Title
Database
Publisher
Elsevier B.V
Data Source
Authors
Cortada,C., dos Reis,L.C., Vidal,L., Llorca,J., Canals,A.
Original/Translated Title
URL
Date of Electronic
20131202
PMCID
Editors
Fungal infectivities of implanted catheters due to Candida sp. Biofilms formation and resistance 2015 Laboratoire antibiotique antifongique, physico-chimie, synthese et activite biologique, universite de Tlemcen, BP 119, 13000 Imama, Tlemcen, Algerie; University center of Naama, BP 66, 45000 Naama, Algerie. Electronic address: seddiki.med@gmail.com.; Labo
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal de mycologie medicale
Periodical, Abbrev.
J.Mycol.Med.
Pub Date Free Form
Jun
Volume
25
Issue
2
Start Page
130
Other Pages
135
Notes
CI: Copyright (c) 2015; JID: 9425651; 0 (Antifungal Agents); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); OTO: NOTNLM; 2014/11/25 [received]; 2015/03/01 [revised]; 2015/03/02 [accepted]; 2015/05/06 [aheadofprint]; ppublish
Place of Publication
France
ISSN/ISBN
1773-0449; 1156-5233
Accession Number
PMID: 25957711
Language
fre
SubFile
English Abstract; Journal Article; IM
DOI
10.1016/j.mycmed.2015.03.003 [doi]
Output Language
Unknown(0)
PMID
25957711
Abstract
BACKGROUND: Candidemia are the most common fungal infections in hospitals. However, the catheters are subject to be altered by Candida biofilms which increase the risk of invasive nosocomial infections due to the high resistance to antifungal agents. Therefore, the minimum inhibitory concentrations of planktonic (MIC) and sessile cells (CIMS) were evaluated. METHODS: To review the in vivo biofilms structures of Candida sp. formed on the inner and/or external surfaces of collected catheters, we used scanning electron microscopy (SEM). The level of biofilm resistance was assessed against two conventional antifungal agents: amphotericin B (AmB), which belongs to the class of polyenes, and fluconazole (FLZ) which is an azole. RESULTS: The SEM observation of biofilms of Candida sp. reveals complex structures. Compared to MICs, the calculation of CIMS showed an increase of 32 times with AmB and of 128 times with FLZ. CONCLUSION: Catheters offer an ideal surface to Candida sp. to form biofilms. This complex structure induces the increase of the resistance of sessile cells against two antifungal agents, AmB and FLZ.
Descriptors
Links
Book Title
Database
Publisher
Elsevier Masson SAS
Data Source
Authors
Seddiki,S.M., Boucherit-Otmani,Z., Boucherit,K., Kunkel,D.
Original/Translated Title
Infectivites fongiques des catheters implantes dues a Candida sp. Formation des biofilms et resistance
URL
Date of Electronic
20150506
PMCID
Editors
Polyphasic study of the spatial distribution of microorganisms in Mexican pozol, a fermented maize dough, demonstrates the need for cultivation-independent methods to investigate traditional fermentations 1999 Laboratoire de Biotechnologie Microbienne Tropicale, Institut de Recherche pour le Developpement, F-34032 Montpellier cedex 1, France. Frederic.Ampe@mpl.ird.fr
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Applied and Environmental Microbiology
Periodical, Abbrev.
Appl.Environ.Microbiol.
Pub Date Free Form
Dec
Volume
65
Issue
12
Start Page
5464
Other Pages
5473
Notes
LR: 20140615; GENBANK/AF138777; GENBANK/AF138778; GENBANK/AF138779; GENBANK/AF138780; GENBANK/AF138781; GENBANK/AF138782; GENBANK/AF138783; GENBANK/AF138784; GENBANK/AF138785; GENBANK/AF138786; GENBANK/AF138787; GENBANK/AF138788; JID: 7605801; 0 (DNA, Bac
Place of Publication
UNITED STATES
ISSN/ISBN
0099-2240; 0099-2240
Accession Number
PMID: 10584005
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
Output Language
Unknown(0)
PMID
10584005
Abstract
The distribution of microorganisms in pozol balls, a fermented maize dough, was investigated by a polyphasic approach in which we used both culture-dependent and culture-independent methods, including microbial enumeration, fermentation product analysis, quantification of microbial taxa with 16S rRNA-targeted oligonucleotide probes, determination of microbial fingerprints by denaturing gradient gel electrophoresis (DGGE), and 16S ribosomal DNA gene sequencing. Our results demonstrate that DGGE fingerprinting and rRNA quantification should allow workers to precisely and rapidly characterize the microbial assemblage in a spontaneous lactic acid fermented food. Lactic acid bacteria (LAB) accounted for 90 to 97% of the total active microflora; no streptococci were isolated, although members of the genus Streptococcus accounted for 25 to 50% of the microflora. Lactobacillus plantarum and Lactobacillus fermentum, together with members of the genera Leuconostoc and Weissella, were the other dominant organisms. The overall activity was more important at the periphery of a ball, where eucaryotes, enterobacteria, and bacterial exopolysacharide producers developed. Our results also showed that the metabolism of heterofermentative LAB was influenced in situ by the distribution of the LAB in the pozol ball, whereas homolactic fermentation was controlled primarily by sugar limitation. We propose that starch is first degraded by amylases from LAB and that the resulting sugars, together with the lactate produced, allow a secondary flora to develop in the presence of oxygen. Our results strongly suggest that cultivation-independent methods should be used to study traditional fermented foods.
Descriptors
Agriculture/methods, Bacteria/classification/genetics/isolation & purification, DNA Fingerprinting, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fermentation, Mexico, Polymerase Chain Reaction, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Zea mays/microbiology
Links
Book Title
Database
Publisher
Data Source
Authors
Ampe,F., ben Omar,N., Moizan,C., Wacher,C., Guyot,J. P.
Original/Translated Title
URL
Date of Electronic
PMCID
PMC91745
Editors
Bis(1-methyl-piperazine-1,4-diium) di-mu-bromido-bis-[tetra-bromido-bismuthate(III)] dihydrate 2014 Laboratoire de Chimie des Materiaux, Faculte des Sciences de Bizerte, 7021 Zarzouna Bizerte, Tunisia.; Centre de Diffractometrie X, UMR 6226 CNRS, Unite Sciences Chimiques de Rennes, Universite de Rennes I, 263 Avenue du General Leclerc, 35042 Rennes, Fra
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Acta crystallographica.Section E, Structure reports online
Periodical, Abbrev.
Acta Crystallogr.Sect.E.Struct.Rep.Online
Pub Date Free Form
10-May
Volume
70
Issue
Pt 6
Start Page
m202
Other Pages
3
Notes
LR: 20140620; JID: 101089178; OID: NLM: PMC4051086; 2014/06/01 [ecollection]; 2014/04/29 [received]; 2014/04/30 [accepted]; 2014/05/10 [epublish]; epublish
Place of Publication
United States
ISSN/ISBN
1600-5368; 1600-5368
Accession Number
PMID: 24940195
Language
eng
SubFile
Journal Article
DOI
10.1107/S1600536814009805 [doi]
Output Language
Unknown(0)
PMID
24940195
Abstract
In the title hydrated salt, (C5H14N2)2[Bi2Br10].2H2O, the com-plete [Bi2Br10](4-) biocta-hedron is generated by crystallographic inversion symmetry. The diprotonated piperazine ring adopts a chair conformation, with the methyl group occupying an equatorial position. In the crystal, the tetra-anions and water mol-ecules are linked by O-Hcdots, three dots, centeredBr and O-Hcdots, three dots, centered(Br,Br) hydrogen bonds to generate [100] chains. The chains are crosslinked by N-Hcdots, three dots, centeredBr, N-Hcdots, three dots, centeredO and C-Hcdots, three dots, centeredBr hydrogen bonds originating from the piperazinediium dications, thereby forming a three-dimensional network.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Essid,M., Roisnel,T., Marouani,H.
Original/Translated Title
URL
Date of Electronic
20140510
PMCID
PMC4051086
Editors
Molecular identification of the microbiota of French sourdough using temporal temperature gradient gel electrophoresis 2007 Laboratoire de Microbiologie Alimentaire et Industrielle (LMAI), Unite de Recherche QM2A, ENITIAA, rue de la Geraudiere, BP 82225, 44322, Nantes Cedex 3, France. mounir.ferchichi@yahoo.fr
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Food Microbiology
Periodical, Abbrev.
Food Microbiol.
Pub Date Free Form
Oct-Dec
Volume
24
Issue
8-Jul
Start Page
678
Other Pages
686
Notes
JID: 8601127; 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); 2007/01/18 [received]; 2007/04/17 [revised]; 2007/04/19 [accepted]; 2007/05/03 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
0740-0020; 0740-0020
Accession Number
PMID: 17613364
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
S0740-0020(07)00057-3 [pii]
Output Language
Unknown(0)
PMID
17613364
Abstract
The microbiota of four industrial French sourdoughs (BF, GO, VB and RF) was characterized by PCR temporal temperature gel electrophoresis (TTGE). The TTGE technique reveals differences in the 16S rDNA V6-V8 regions of these bacteria. DNA was extracted directly from sourdough samples. A specific TTGE fingerprint was determined for 30 bacterial species, including members of the genera Lactobacillus, Leuconostoc and Weissella, all known to be present in sourdough. These sourdoughs contain different species of lactic acid bacteria (LAB) depending on ecological conditions prevailing in the different sourdough fermentations. Only a few LAB species were found to be competitive and became dominant. Lactobacillus sanfranciscensis was observed as the most frequently found species. In sourdough GO, L. sanfranciscensis, Lactobacillus panis and two new species, Lactobacillus nantensis and Lactobacillus hammesii, were detected. Sourdough BF contain L. sanfranciscensis, Lactobacillus spicheri and Lactobacillus pontis. In sourdough VB, which differed in the process temperature, we identified exclusively L. sanfranciscensis and Leuconostoc mesenteroides subsp. mesenteroides. Lactobacillus frumenti, L. hammesii and Lacobacillus paralimentarius became the predominant species in sourdough RF. Compared with conventional bacteriological methods, the use of this new molecular approach to analyze the sourdough ecosystem should therefore allow a more complete and rapid assessment of its specific microbiota.
Descriptors
Bread/microbiology, Colony Count, Microbial, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Electrophoresis, Agar Gel/methods, Fermentation, Food Microbiology, Lactobacillus/classification/isolation & purification, Leuconostoc/classification/isolation & purification, Phylogeny, Polymerase Chain Reaction/methods, RNA, Ribosomal, 16S/chemistry/genetics, Species Specificity, Temperature
Links
Book Title
Database
Publisher
Data Source
Authors
Ferchichi,M., Valcheva,R., Prevost,H., Onno,B., Dousset,X.
Original/Translated Title
URL
Date of Electronic
20070503
PMCID
Editors