WTS database

Title	Pub Year	Author	SearchLink Sort ascending
Prediction equations for pulmonary function values in healthy young Iranians aged 8-18 years	2004	Department of Physiology, Ghaem Medical Centre, Mashhad University of Medical Sciences, Mashhad, Iran. mhboskabady@hotmail.com	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Respirology (Carlton, Vic.) Periodical, Abbrev. Respirology Pub Date Free Form Nov Volume 9 Issue 4 Start Page 535 Other Pages 542 Notes LR: 20061115; JID: 9616368; ppublish Place of Publication Australia ISSN/ISBN 1323-7799; 1323-7799 Accession Number PMID: 15612967 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; Validation Studies; IM DOI RES623 [pii] Output Language Unknown(0) PMID 15612967 Abstract OBJECTIVE: Pulmonary function test (PFT) variables are dependent on height, age and gender. In addition, there is evidence of PFT variation in different ethnic groups. Prediction equations for PFT from a healthy, non-smoking, urban young population in the city of Mashhad (north-east Iran) have been derived. METHODOLOGY: Prediction equations for normal pulmonary function were derived from 336 healthy, non-smoking subjects, including 187 males (height 103-188.5 cm) and 149 females (height 104-183 cm) aged 8-18 years. The subjects underwent measurement of spirometric flow and volume. The following variables were measured: FVC, FEV1, maximal mid-expiratory flow (MMEF), PEF, maximal expiratory flow at 75, 50 and 25% of the FVC (MEF75, MEF50, and MEF25, respectively), tidal volume (VT), inspiratory reserve volume (IRV), expiratory reserve volume (ERV), inspiratory capacity (IC), and vital capacity (VC). Regression analysis using height and age as independent variables was applied to provide predicted values for both genders. RESULTS: There were positive correlations for each pulmonary function variable with height and age. The largest positive correlations were found for FEV1 with height and age, in both genders. Comparison of PFT variables derived from the equations obtained in the present study showed significant differences to those calculated from several previously published equations (P < 0.001 for most variables). For example, the values of FVC and FEV1 derived from the equations obtained in the present study were 2.83 +/- 0.99 and 2.50 +/- 0.89 for males, and 2.41 +/- 0.54 and 2.19 +/- 0.53 for females, while the values derived from the equations of the European Community for Steel and Coal study were 3.12 +/- 1.06 and 2.62 +/- 0.89 for males and 2.79 +/- 0.67 and 3.35 +/- 0.57 for females, respectively. CONCLUSIONS: A set of PFT reference values and prediction equations for both genders has been derived using a relatively large, healthy, non-smoking Iranian young population, and has generated results that differ from several other prediction equations. Descriptors Adolescent, Age Factors, Body Height, Child, Female, Humans, Iran, Lung/physiology, Male, Predictive Value of Tests, Reference Values, Regression Analysis, Reproducibility of Results, Respiratory Function Tests/standards, Sex Factors, Urban Population Links Book Title Database Publisher Data Source Authors Boskabady,M. H., Tashakory,A., Mazloom,R., Ghamami,G. Original/Translated Title URL Date of Electronic PMCID Editors
Does stage-based smoking cessation advice in pregnancy result in long-term quitters? 18-month postpartum follow-up of a randomized controlled trial	2005	Department of Public Health and Epidemiology, University of Birmingham, Birmingham UK.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Addiction (Abingdon, England) Periodical, Abbrev. Addiction Pub Date Free Form Jan Volume 100 Issue 1 Start Page 107 Other Pages 116 Notes LR: 20071115; JID: 9304118; ppublish Place of Publication England ISSN/ISBN 0965-2140; 0965-2140 Accession Number PMID: 15598198 Language eng SubFile Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't; IM DOI ADD936 [pii] Output Language Unknown(0) PMID 15598198 Abstract AIMS: To evaluate the effect on quitting smoking at 18 months postpartum of smoking cessation interventions based on the Transtheoretical Model (TTM) delivered in pregnancy compared to current standard care. It has been claimed that TTM-based interventions will continue to create quitters after the end of the intervention period. DESIGN: Cluster randomized trial. SETTING: Antenatal clinics in general practices in the West Midlands, UK. PARTICIPANTS: A total of 918 pregnant smokers originally enrolled in the trial, of which 393 women were followed-up at 18 months postpartum. INTERVENTIONS: One hundred general practices were randomized into the three trial arms. Midwives in these practices delivered three interventions: A (standard care), B (TTM-based self-help manuals) and C (TTM-based self-help manuals plus sessions with an interactive computer program giving individualized smoking cessation advice). MEASUREMENTS: Self-reported continuous and point prevalence abstinence since pregnancy. FINDINGS: When combined together, there was a slight and not significant benefit for both TTM arms compared to the control, with an odds ratio (OR) 95% confidence interval (CI) of 1.20 (0.29-4.88) for continuous abstinence. For point prevalence abstinence, the OR (95%CI) was 1.15 (0.66-2.03). Seven of the 54 (13%) women who had quit at the end of pregnancy were still quit 18 months later, and there was no evidence that the TTM-based interventions were superior in preventing relapse. CONCLUSIONS: The TTM-based interventions may have shown some evidence of a short-term benefit for quitting in pregnancy but no benefit relative to standard care when followed-up in the longer-term. Descriptors Adult, Behavior Therapy/methods, Cluster Analysis, Confidence Intervals, Female, Follow-Up Studies, Health Promotion, Humans, Odds Ratio, Patient Compliance, Patient Education as Topic/methods, Pregnancy, Pregnancy Complications/prevention & control, Smoking/prevention & control, Smoking Cessation/methods/psychology, Treatment Outcome Links Book Title Database Publisher Data Source Authors Lawrence,T., Aveyard,P., Cheng,K. K., Griffin,C., Johnson,C., Croghan,E. Original/Translated Title URL Date of Electronic PMCID Editors
Headspace solid-phase microextraction as a tool to estimate the contamination of smoked cheeses by polycyclic aromatic hydrocarbons	2005	Tecnologia de Alimentos, Facultad de Farmacia, Universidad del Pais Vasco, Paseo de la Universidad, Vitoria, Spain. knpgulod@vf.ehu.es	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Journal of dairy science Periodical, Abbrev. J.Dairy Sci. Pub Date Free Form Jan Volume 88 Issue 1 Start Page 13 Other Pages 20 Notes LR: 20061115; JID: 2985126R; 0 (Polycyclic Hydrocarbons, Aromatic); 0 (Smoke); ppublish Place of Publication United States ISSN/ISBN 0022-0302; 0022-0302 Accession Number PMID: 15591362 Language eng SubFile Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; IM DOI S0022-0302(05)72657-6 [pii] Output Language Unknown(0) PMID 15591362 Abstract Headspace solid-phase microextraction (HS-SPME) was used to study polycyclic aromatic hydrocarbons (PAH) in smoked cheeses. Two types of fiber coatings and different extraction conditions were tested. The results reveal that the use of an 85-microm polyacrylate fiber immersed in the headspace of the samples at 70 degrees C for 60 min is suitable for the detection of PAH with no more than 4 aromatic rings. To determine if a relationship can be established between the results obtained using a solvent extraction technique and HS-SPME, 6 samples of smoked cheese previously studied by a solvent extraction method were analyzed by HS-SPME, and the results obtained by both methodologies were compared. Polycyclic aromatic hydrocarbons were identified and quantified by gas chromatography-mass spectrometry operating in selective ion monitoring mode. Among the PAH determined by the solvent extraction method, only those with 4 aromatic rings or less were detected by HS-SPME and, consequently, this technique does not allow one to determine the PAH content of smoked cheese samples under the conditions of the study. Nevertheless, the relationship between the results obtained by both techniques for some PAH revealed that HS-SPME could be useful as a screening method to distinguish among samples with different degrees of PAH contamination. Descriptors Cheese/analysis, Chromatography, Gas/methods, Food Contamination/analysis, Food Handling/methods, Gas Chromatography-Mass Spectrometry, Polycyclic Hydrocarbons, Aromatic/analysis, Smoke Links Book Title Database Publisher Data Source Authors Guillen,M. D., Sopelana,P. Original/Translated Title URL Date of Electronic PMCID Editors
Review article: Barrett's oesophagus and carcinoma in Japan	2004	Department of Comprehensive Medicine and Psychosomatic Medicine, Tohoku University School of Medicine, Sendai 980-8574, Japan. m-hongo@mail.tains.tohoku.ac.jp	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Alimentary Pharmacology & Therapeutics Periodical, Abbrev. Aliment.Pharmacol.Ther. Pub Date Free Form Dec Volume 20 Suppl 8 Issue Start Page 50 Other Pages 54 Notes LR: 20051116; JID: 8707234; RF: 31; ppublish Place of Publication England ISSN/ISBN 0269-2813; 0269-2813 Accession Number PMID: 15575874 Language eng SubFile Journal Article; Review; IM DOI APT2230 [pii] Output Language Unknown(0) PMID 15575874 Abstract In Asia, oesophageal diseases, such as Barrett's oesophagus and oesophageal carcinoma, have traditionally been less common than in America and Europe. In recent years, however, the number of reported cases of these conditions in Japan has increased. Two large prospective studies, the Sendai Barrett's Esophagus Study (S-BEST) and the Far East Study (FEST), on the geographic prevalence of Barrett's oesophagus, have recently investigated the epidemiology of Barrett's oesophagus in Japan. Results from both studies showed that overall prevalence of the condition is lower than in the West: 0.9-1.2% in Japan compared with 1-4% in Europe and 5-12% in USA. Similar to the situation in the West, the condition was shown to be most prevalent in elderly male patients and least prevalent in patients with Helicobacter pylori. Adenocarcinoma of the oesophagus is still rare in this region, although there has been an increase in the annual death rate from 3.7 (1960) to 6.9 (1995) per 100,000 population. Risk factors for oesophageal carcinoma include a strong association with the prevalence of gastro-oesophageal reflux disease (GERD). With the increasing prevalence of GERD in the Japanese population, continued surveillance of changes in the epidemiology of columnar-lined oesophagus (a precursor of Barrett's oesophagus), Barrett's oesophagus and adenocarcinoma of the oesophagus is strongly recommended. Descriptors Adenocarcinoma/epidemiology/pathology, Barrett Esophagus/epidemiology/pathology, Esophageal Neoplasms/epidemiology/pathology, Gastroesophageal Reflux/epidemiology/pathology, Humans, Japan/epidemiology, Prevalence Links Book Title Database Publisher Data Source Authors Hongo,M. Original/Translated Title URL Date of Electronic PMCID Editors
Resin bond strength and micromorphology of human teeth prepared with an Erbium:YAG laser	2004	Department of Restorative Dentistry and Biomaterials, University of Texas Dental Branch at Houston, 6516 M.D. Anderson Blvd., Suite 493, Houston, Texas 77030-3402, USA. Cynthia.Trajtenberg@uth.tmc.edu	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full American Journal of Dentistry Periodical, Abbrev. Am.J.Dent. Pub Date Free Form Oct Volume 17 Issue 5 Start Page 331 Other Pages 336 Notes LR: 20131121; JID: 8806701; 0 (Composite Resins); 0 (Dentin-Bonding Agents); 0 (Filtek Z250); 0 (Resin Cements); 0 (Scotchbond Multi-Purpose Plus); 77B218D3YE (Erbium); ppublish Place of Publication United States ISSN/ISBN 0894-8275; 0894-8275 Accession Number PMID: 15575443 Language eng SubFile Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; D DOI Output Language Unknown(0) PMID 15575443 Abstract PURPOSE: To evaluate the in vitro effect of the Er:YAG laser and high-speed rotary instrumentation on the bond strength of resin composite to human enamel and dentin, and determine which conditioner, either phosphoric acid or a self-etching primer, resulted in higher bond strengths with either of the surface treatments. METHODS: 48 third molars were used. Dentin and enamel specimens were sectioned and polished with 600-grit SiC paper and treated either with carbide bur or an Erbium:YAG laser and treated with one of three different conditions, acid-etch/bonding agent (Scotchbond Multi-Purpose Plus), no etching and same bonding agent, and an experimental self-etching primer (EXL 547). After storage for 48 hours at 37 degrees C and 100% humidity, specimens were prepared in an hourglass shape for microtensile bond test (ca. 1 mm2) and debonded in tension. Areas were measured and bond strengths were calculated for each specimen. Failure modes, micromorphology of surface treatments and bonding interfaces of representative specimens from acid-etched and self-etched groups were analyzed with scanning electron microscopy. Means were compared using three-way analysis of variance, and Scheffe post-hoc test (P < 0.05) was used to determine differences among surface treatments, tooth substrate and conditioners. RESULTS: Dentin prepared with the carbide bur and treated with phosphoric acid followed by the application of Scotchbond Multi-Purpose had the highest bond strengths (35.7 MPa). Enamel and dentin prepared with the Er:YAG laser had the highest bond strengths when the surfaces were acid-etched followed by Scotchbond Multi-Purpose (25.8-21.1 MPa). Carbide bur exhibited higher bond strengths than laser with the use of the experimental self-etching primer but laser showed higher bond strengths than the bur with the use of Scotchbond Multi-Purpose and no etching. The predominant failure mode of most of the treatment conditions was partially adhesive between the bonding resin and enamel or dentin, and partially cohesive within the bonding resin. SEM analysis revealed the absence of a smear layer on laser-treated teeth when compared to untreated control and bur-cut teeth. Descriptors Acid Etching, Dental/methods, Analysis of Variance, Composite Resins, Dental Bonding, Dental Cavity Preparation/methods, Dental Enamel/ultrastructure, Dental High-Speed Technique, Dental Stress Analysis, Dentin/ultrastructure, Dentin-Bonding Agents, Erbium, Humans, Lasers, Materials Testing, Molar, Resin Cements, Smear Layer, Statistics, Nonparametric, Tensile Strength Links Book Title Database Publisher Data Source Authors Trajtenberg,C. P., Pereira,P. N., Powers,J. M. Original/Translated Title URL Date of Electronic PMCID Editors
Multiethnic differences in smoking in Israel: pooled analysis from three national surveys	2004	Israel Center for Disease Control, Ministry of Health, Tel Hashomer, Israel. ornaepel@research.haifa.ac.il	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full European journal of public health Periodical, Abbrev. Eur.J.Public Health Pub Date Free Form Dec Volume 14 Issue 4 Start Page 384 Other Pages 389 Notes LR: 20061115; JID: 9204966; ppublish Place of Publication England ISSN/ISBN 1101-1262; 1101-1262 Accession Number PMID: 15542874 Language eng SubFile Comparative Study; Journal Article; IM DOI 14/4/384 [pii] Output Language Unknown(0) PMID 15542874 Abstract BACKGROUND: Smoking is a major public health risk and information regarding high-risk groups is needed to plan, implement and evaluate interventions aimed at lowering the number of smokers. METHODS: During the years 1999-2001 data was collected regarding the smoking behaviour of the Israeli population in three national surveys. All three surveys included ages 25-64 and two included also ages 21-25 and over 64. RESULTS: Smoking was associated with age, sex, ethnicity, education and religiosity after adjusting for the various demographic characteristics of the survey population. The prevalence of smoking among Arab and immigrant men from the former Soviet Union is higher than among Jewish men. Among women the opposite association exists. The older, religious and more educated reported smoking less frequently. In the Jewish population respondents, defining themselves as secular, reported higher rates of smoking. Only in women was marital status associated with smoking. A few specific high-risk groups for smoking can be identified such as young, less educated men, Arab men, single Jewish women and young immigrant men and women. CONCLUSIONS: The three ethnic groups residing in Israel differ in the prevalence of smoking; each has a distinct pattern of smoking, positioning them at different stages within the conceptual framework of the larger smoking pandemic. Ethnicity, religiosity, age and education are associated with smoking in both sexes. This calls for specific tailored interventions aimed at younger men with less education, Arab men, and young immigrants. Descriptors Adult, Age Distribution, Aged, Arabs/psychology/statistics & numerical data, Emigration and Immigration/statistics & numerical data, Female, Health Behavior/ethnology, Health Surveys, Humans, Israel/epidemiology, Jews/psychology/statistics & numerical data, Logistic Models, Male, Marital Status, Middle Aged, Prevalence, Risk Factors, Sex Distribution, Smoking/epidemiology/ethnology, USSR/ethnology Links Book Title Database Publisher Data Source Authors Baron-Epel,O., Haviv-Messika,A., Tamir,D., Nitzan-Kaluski,D., Green,M. Original/Translated Title URL Date of Electronic PMCID Editors
Determination of benzene, toluene, ethylbenzene and xylenes in air by solid phase micro-extraction/gas chromatography/mass spectrometry	2004	Laboratoire de Radiochimie, Sciences Analytiques et Environnement, Faculte des Sciences, Universite de Nice-Sophia Antipolis, Parc Valrose, 06108 Nice, France.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Analytical and bioanalytical chemistry Periodical, Abbrev. Anal.Bioanal Chem. Pub Date Free Form Nov Volume 380 Issue 6-May Start Page 824 Other Pages 830 Notes LR: 20160512; JID: 101134327; 0 (Air Pollutants); 0 (Benzene Derivatives); 0 (Xylenes); 3FPU23BG52 (Toluene); J64922108F (Benzene); L5I45M5G0O (ethylbenzene); ppublish Place of Publication Germany ISSN/ISBN 1618-2642; 1618-2642 Accession Number PMID: 15517200 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1007/s00216-004-2837-1 [doi] Output Language Unknown(0) PMID 15517200 Abstract The aim of the study was to analyse BTEX compounds (benzene, toluene, ethylbenzene, xylenes) in air by solid phase micro-extraction/gas chromatography/mass spectrometry (SPME/GC/MS), and this article presents the features of the calibration method proposed. Examples of real-world air analysis are given. Standard gaseous mixtures of BTEX in air were generated by dynamic dilution. SPME sampling was carried out under non-equilibrium conditions using a Carboxen/PDMS fibre exposed for 30 min to standard gas mixtures or to ambient air. The behaviour of the analytical response was studied from 0 to 65 microg/m3 by adding increasing amounts of BTEX to the air matrix. Detection limits range from 0.05 to 0.1 microg/m3 for benzene, depending on the fibre. Inter-fibre relative standard deviations (reproducibility) are larger than 18%, although the repeatability for an individual fibre is better than 10%. Therefore, each fibre should be considered to be a particular sampling device, and characterised individually depending on the required accuracy. Sampling indoor and outdoor air by SPME appears to be a suitable short-delay diagnostic method for volatile organic compounds, taking advantage of short sampling time and simplicity. Descriptors Air Pollutants/analysis, Benzene/analysis, Benzene Derivatives/analysis, Calibration, Environmental Monitoring, Gas Chromatography-Mass Spectrometry/methods, Time Factors, Toluene/analysis, Xylenes/analysis Links Book Title Database Publisher Data Source Authors Tumbiolo,S., Gal,J. F., Maria,P. C., Zerbinati,O. Original/Translated Title URL Date of Electronic PMCID Editors
Smoking at home is strongly associated with symptoms of asthma and rhinitis in children of primary school age in Trinidad and Tobago	2004	University of the West Indies, Faculty of Medical Sciences-St. Augustine Campus, Champs Fleurs, Trinidad. mmonteil@tstt.net.tt	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Revista panamericana de salud publica = Pan American journal of public health Periodical, Abbrev. Rev.Panam.Salud Publica Pub Date Free Form Sep Volume 16 Issue 3 Start Page 193 Other Pages 198 Notes LR: 20151119; JID: 9705400; 0 (Tobacco Smoke Pollution); ppublish Place of Publication United States ISSN/ISBN 1020-4989; 1020-4989 Accession Number PMID: 15507187 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI S1020-49892004000900006 [pii] Output Language Unknown(0) PMID 15507187 Abstract OBJECTIVE: To compare, in the twin-island republic of Trinidad and Tobago, the prevalence of symptoms of asthma and rhinitis among children of primary school age who are exposed to household environmental tobacco smoke with the prevalence of these symptoms in their colleagues without this exposure. METHODS: Between September and December 2002, questionnaires based on the instrument developed for the International Study of Asthma and Allergies in Childhood (ISAAC) were distributed, via the children in their schools, to parents of 6,611 Year 2 pupils (typically 6 years old) or Year 3 pupils (typically 7 years old) in 106 randomly selected schools in Trinidad and Tobago (5,511 pupils on Trinidad and 1,100 pupils on Tobago). We added to the standard ISAAC questionnaires two questions, one on household smoking and one on the ethnicity of the children. RESULTS: A total of 3 170 completed questionnaires were suitable for further analysis (2,618 from Trinidad and 552 from Tobago). On Trinidad 782 of the children (29.9%) lived in homes where one or both parents smoked, and 513 (19.6%) had other relatives in the household who smoked. On Tobago 94 of the pupils (17.0%) had parents who smoked, and 84 (15.4%) came from homes where other residents smoked. Parental smoking was significantly associated with wheezing (odds ratio (OR): 1.43; 95% confidence interval (CI): 1.11-1.83), exercise-induced wheezing (OR: 2.12; 95% CI: 1.59-2.82), nocturnal coughing (OR: 1.64; 95% CI: 1.37-1.97), and symptoms of rhinitis (OR: 1.35; 95% CI: 1.10-1.65) in the last 12 months as well as a history of hay fever/sinus problems (OR: 1.39; 95% CI: 1.11-1.74). Smoking in the home by adult residents other than parents was also significantly associated with all of these symptoms as well as a history of asthma (OR: 1.49; 95% CI: 1.13-1.97). In terms of ethnic differences, parental smoking was most prevalent in the homes of South Asian students, while smoking by other adults in the home occurred most commonly in the households of pupils of mixed race. CONCLUSIONS: Even in Trinidad and Tobago, which is a tropical environment where more time is spent outdoors and homes have more open ventilation than in temperate climates, environmental tobacco smoke exposure is closely associated with an increased prevalence of symptoms of asthma and rhinitis in primary-school-aged children. Descriptors Adult, Asthma/epidemiology/etiology, Child, Cough, Ethnic Groups, Female, Humans, Male, Prevalence, Respiratory Sounds, Rhinitis, Allergic, Perennial/epidemiology/etiology, Rhinitis, Allergic, Seasonal/epidemiology/etiology, Sampling Studies, Sinusitis/epidemiology, Surveys and Questionnaires, Tobacco Smoke Pollution/adverse effects, Trinidad and Tobago/epidemiology Links Book Title Database Publisher Data Source Authors Monteil,M. A., Joseph,G., Chang Kit,C., Wheeler,G., Antoine,R. M. Original/Translated Title URL Date of Electronic PMCID Editors
In vitro pharmacodynamic characteristics of amphotericin B, caspofungin, fluconazole, and voriconazole against bloodstream isolates of infrequent Candida species from patients with hematologic malignancies	2004	Center of Excellence on Aging, Room 27, 5th level, "G. D'Annunzio" University, Via Colle dell'Ara, 66100 Chieti, Italy.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Antimicrobial Agents and Chemotherapy Periodical, Abbrev. Antimicrob.Agents Chemother. Pub Date Free Form Nov Volume 48 Issue 11 Start Page 4453 Other Pages 4456 Notes LR: 20141120; JID: 0315061; 0 (Antifungal Agents); 0 (Echinocandins); 0 (Peptides, Cyclic); 0 (Pyrimidines); 0 (Triazoles); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); F0XDI6ZL63 (caspofungin); JFU09I87TR (Voriconazole); OID: NLM: PMC525414; pp Place of Publication United States ISSN/ISBN 0066-4804; 0066-4804 Accession Number PMID: 15504881 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 48/11/4453 [pii] Output Language Unknown(0) PMID 15504881 Abstract Time-kill and postantifungal effect (PAFE) of amphotericin B, caspofungin, fluconazole, and voriconazole were determined against clinical isolates of Candida guilliermondii, Candida kefyr, and Candida lusitaniae. Azoles displayed fungistatic activity and no measurable PAFE, regardless of the concentration tested. Amphotericin B and caspofungin demonstrated concentration-dependent fungicidal activity, although amphotericin B only produced a significant dose-dependent PAFE against all isolates tested. Descriptors Amphotericin B/pharmacology, Antifungal Agents/pharmacology, Candida/drug effects, Candidiasis/complications/microbiology, Colony Count, Microbial, Echinocandins, Fluconazole/pharmacology, Hematologic Neoplasms/complications, Humans, Kinetics, Microbial Sensitivity Tests, Models, Biological, Peptides, Cyclic/pharmacology, Pyrimidines/pharmacology, Triazoles/pharmacology, Voriconazole Links Book Title Database Publisher Data Source Authors Di Bonaventura,G., Spedicato,I., Picciani,C., D'Antonio,D., Piccolomini,R. Original/Translated Title URL Date of Electronic PMCID PMC525414 Editors
Identification and expression of blaCTX-M-14 and blaCTX-M-24	2004	Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, China.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Zhonghua yi xue za zhi Periodical, Abbrev. Zhonghua Yi Xue Za Zhi Pub Date Free Form 2-Sep Volume 84 Issue 17 Start Page 1454 Other Pages 1459 Notes LR: 20131121; JID: 7511141; 0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); EC 3.5.2.- (beta-lactamase CTX-M-14); EC 3.5.2.6 (beta-Lactamases); N2GI8B1GK7 (Cefotaxime); ppublish Place of Publication China ISSN/ISBN 0376-2491; 0376-2491 Accession Number PMID: 15500745 Language chi SubFile English Abstract; Journal Article; Research Support, Non-U.S. Gov't; IM DOI Output Language Unknown(0) PMID 15500745 Abstract OBJECTIVE: To identify the ESBL gene and the prevalence in Escherichia coli and Klebsiella pneumoniae strain isolated from Huashan Hospital, Shanghai. METHODS: Isolates were confirmed as an ESBL producing strain by double-disk synergy test and NCCLS Confirmatory Test. Antibiotic susceptibilities were determined by standard agar dilution procedure on Mueller-Hinton agar. To determine whether the resistance was transferable, the conjugation experiment was performed; plasmids were isolated from clinical isolates and transcojugants. The partial bla(gene) of ESBL producing isolates and their transcojugants were detected by PCR using universal primers for TEM, SHV, CTX-M-1group, Toho-1group, CTX-M-13group respectively. The entire bla(CTX-M-13) group were amplified by PCR using the primers outside the Open Reading Frame (ORF) of CTX-M-13group beta-lactamases; the PCR products of entire bla(CTX-M-13)group were cloned into vector and the recombinant plasmids were transformed into the recipient strain for expression; the PCR products were also directly sequenced and analyzed; the clinical isolates of ESBL producers were detected by PFGE. RESULTS: ESBL producers were resistant to most beta-lactams and non-beta-lactams. Most transconjugants were obtained at frequency of 10(-4) approximately 10(-5) and resistance to non-beta-lactams was cotransferred with the ESBL activity to the transconjugant. A plasmid of about > 23.1 kb was obtained from each tansconjugant by plasmid extraction. Partial gene amplification products of CTX-M-13 group gene were obtained from isolates and their transconjugants. The bla(CTX-M-13)group from 4 transconjugants were identified as bla(CTX-M-14), and other six were bla(CTX-M-24); those ESBLs were mediated by plasmids (> 23.1 kb); the transformants producing CTX-M-14 or CTX-M-24 were resistant to most beta-lactams, which were much more resistant to cefotaxime than to ceftazidine; PFGE patterns of those isolates were different. CONCLUSION: clinical isolate of Escherichia coli and Klebsiella pneumoniae isolated from Huashan Hospital, Shanhai produced CTX-M-14 or CTX-M-24, which caused the isolate resistant to most beta-lactams; no clone spread in those isolates was found. Descriptors Anti-Bacterial Agents/pharmacology, Cefotaxime/pharmacology, DNA, Bacterial/genetics, Drug Resistance, Bacterial/genetics, Escherichia coli/enzymology/genetics, Escherichia coli Infections/microbiology, Genes, Bacterial/genetics, Humans, Klebsiella Infections/microbiology, Klebsiella pneumoniae/enzymology/genetics, beta-Lactam Resistance/genetics, beta-Lactamases/genetics/metabolism Links Book Title Database Publisher Data Source Authors Xiong,Z. Z., Zhu,D. M., Wang,F., Zhang,Y. Y. Original/Translated Title URL Date of Electronic PMCID Editors

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