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Microbial diversity of a community for ensiling rice straw at low temperature and fermentation dynamics 2011 College of Life Sciences, Northeast Forestry University, Harbin 150040, China. ayanghy@yahoo.com.cn
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Journal Article
Periodical, Full
Wei sheng wu xue bao = Acta microbiologica Sinica
Periodical, Abbrev.
Wei Sheng Wu Xue Bao
Pub Date Free Form
Sep
Volume
51
Issue
9
Start Page
1248
Other Pages
1255
Notes
LR: 20151119; JID: 21610860R; 0 (RNA, Ribosomal, 16S); ppublish
Place of Publication
China
ISSN/ISBN
0001-6209; 0001-6209
Accession Number
PMID: 22126081
Language
chi
SubFile
English Abstract; Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
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Unknown(0)
PMID
22126081
Abstract
OBJECTIVE: To accelerate the conversion of rice straw into feeds in the low-temperature region, a microbial community was constructed by continuous enrichment cultivation. Microbial diversity and dynamics during the fermentation at 10 degrees C was analyzed. METHODS: The community was selected at 5 degrees C under static condition. To analyze the inoculating effects, the community and commercial inoculant ( CI: composed of Lactobacillus plantarum, Enterococcus faecium, L. salivarilus, Pediococcus acidilactici) were respectively inoculated into the rice straw for 30 d fermentation at 10 degrees C. Fermented products were detected by gas chromatography - mass spectrometry (GC-MS). Composition microorganisms of the community were analyzed using cloning library. Microbial dynamics during the fermentation was detected by denatured gradient gel eletrophoresis (DGGE). Quantitative PCR was used for tracking the composition microorganisms of the community during the fermentation. RESULTS: The results from 16S rDNA cloning library showed that the community was mainly composed of Lactobacillus spp. and Leuconostoc spp. At 6d fermentation, the pH and the lactic acid bacterial colony forming units (LAB CFUs) in the fermented rice straw with the community amounted to 4.3 and 2.9 x 10(9) CFU/g fresh matter (FM), respectively. The pH and LAB CFUs with the CI were respectively 5.3 and 2.9 x 10(9) CFU/g FM. At 30 d fermentation, the lactic acid concentrations with the community and the CI were respectively 8.1 g/kg FM and 2.0 g/kg FM. From DGGE patterns, both L. sakei and Leuconostoc inhae of the community were detected at 6d fermentation and existed during the fermentation. For the treatment with the CI, the uncultured bacterium was detected at 6d fermentation besides the composition microorganisms of the CI. At 16d and 30d fermentation, only L. plantarum and E. faecium were detected. Quantitative PCR showed DNA mass of L. sakei amounted to 41.0% at 6d fermentation in the treatment with the community. At 16d, DNA mass of L. sakei was 65%. The highest value (5.5%) of DNA mass of Le inhae appeared at 6d of fermentation. CONCLUSION: The community could effectively colonize into the rice straw fermentation system and accelerate the fermentation process at low temperature. The dominating microorganism of the community was L. sakei at 10 degrees C.
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Yang,H., Yuan,X., Liu,X., Wang,X., Cui,Z.
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Concentrations and ozone formation potentials of BTEX during 2008-2010 in urban Beijing, China 2013 College of Forestry, Gansu Agricultural University, Lanzhou 730070, China. caohy@dq.cern.ac.cn
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Periodical, Full
Huan jing ke xue= Huanjing kexue / [bian ji, Zhongguo ke xue yuan huan jing ke xue wei yuan hui "Huan jing ke xue" bian ji wei yuan hui.]
Periodical, Abbrev.
Huan Jing Ke Xue.
Pub Date Free Form
Jun
Volume
34
Issue
6
Start Page
2065
Other Pages
2070
Notes
JID: 8405344; 0 (Aerosols); 0 (Air Pollutants); 0 (Benzene Derivatives); 0 (Organic Chemicals); 0 (Xylenes); 3FPU23BG52 (Toluene); 66H7ZZK23N (Ozone); J64922108F (Benzene); L5I45M5G0O (ethylbenzene); ppublish
Place of Publication
China
ISSN/ISBN
0250-3301; 0250-3301
Accession Number
PMID: 23947015
Language
chi
SubFile
English Abstract; Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
Output Language
Unknown(0)
PMID
23947015
Abstract
Benzene, toluene, ethylbenzene and dimethylbenzene are typical anthropogenic emitted organics in the atmosphere, which not only endanger human health but also actively participate in photochemical reactions, generating O3 and secondary organic aerosols. In order to investigate the pollution level and its ozone formation potentials, concentrations of BTEX and O3 were parallel measured by the passive sampler and analyzed using GC-MS and ICS-90 during 2008-2010 in urban Beijing. The results show that toluene was the most abundant compound (8.7 +/- 3.1) microg x m(-3), followed by benzene, ethylbenzene and m/p-xylene, with concentrations of (7.1 +/- 3.3), (4.2 +/- 1.4) and (3.4 +/- 1.5) microg x m(-3), respectively. Concentrations of BTEX peaked in winter, followed by autumn, summer, and spring, with values of (26.8 +/- 12.1), (25.9 +/- 4.9), (24.7 +/- 2.8) and (16.8 +/- 1.4) microg x m(-3), respectively. Benzene concentrations were the highest in winter, while for toluene, the concentration was higher in summer than that in winter. Based on the maximum incremental reactivity scale, m/p-xylene was found to be the dominant contributor to ozone formation among BTEX. The ozone formation potentials of BTEX in Beijing were 65.2, 60.2, and 75.7 microg x m(-3) in 2008, 2009, and 2010, respectively, which were consistent with the measured values of 80.5, 65.0, and 101.9 microg x m(-3) during the corresponding period. Vehicular emission and solvent evaporation were the major sources of BTEX. Concentrations of benzene were affected by coal heating in winter, whereas BTEX concentration was more influenced by solvent evaporation in summer, which had an important contribution to the formation of O3 in urban Beijing.
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Cao,H.Y., Pan,Y.P., Wang,H., Tan,J.H., Wang,Y.S.
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Changes in soil properties and site productivity caused by red alder 1990 College of Forest Resources, University of Washington, Seattle, WA 98195
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Print(0)
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Journal Article
Periodical, Full
Water, air, and soil pollution
Periodical, Abbrev.
Water Air Soil Pollut.
Pub Date Free Form
1991; 1990/
Volume
54
Issue
SPEC. ISS.
Start Page
231
Other Pages
246
Notes
Place of Publication
ISSN/ISBN
0049-6979
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
Red alder (Alnus rubra Bong.) is well recognized as an effective host plant for the symbiotic fixation of N. While this fixation process leads to the rapid accumulation of N within the ecosystem, it also enhances nutrient accumulation in biomass and soil organic matter and increases nitrification and cation leaching. We hypothesized that changes in soil properties resulting from these processes would decrease site productivity for second rotation red alder. Adjacent stands of 55 yr old alder and Douglas fir (Pseudotsuga menziesii [Mirb.] Franco) were studied at the Thompson Research Center on the Cedar River Watershed in western Washington, USA. The presence of red alder caused the following soil changes: decreased soil solution pH, increased CEC, increased exchangeable acidity accompanied by a decreased soil pH and base saturation. This decreased soil and soil solution pH resulted in increased Al concentration in the soil solution and on exchange sites as well as decreased P availability. To determine the effect of these changes on the productivity of the 2nd rotation alder forest, a species conversion experiment was initiated 5 yr ago. Results from this conversion study clearly indicated that the first rotation red alder forest has caused a relative decrease in the productivity of the second rotation red alder plantation. Compared to the growth of red alder on the former Douglas fir site, the second rotation red alder on the former red alder site exhibited 33% less height growth and 75% less aboveground biomass accumulation after 5 yr. Future research will focus on identifying those factors causing this lower productivity including P availability, soil acidity and Al toxicity, cation availability, and competition with other vegetation.
Descriptors
aluminum, nitrate, conference paper, nitrogen fixation, nonhuman, soil, tree
Links
Book Title
Database
Embase
Publisher
Data Source
Embase
Authors
Cole,D. W., Compton,J., Van Miegroet,H., Homann,P.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
The impact of nitrification on soil acidification and cation leaching in a red alder ecosystem 1984 College of Forest Resources, Univ. of Washington, Seattle, WA 98195
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Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of environmental quality
Periodical, Abbrev.
J.Environ.Qual.
Pub Date Free Form
1984/
Volume
13
Issue
4
Start Page
586
Other Pages
590
Notes
Place of Publication
ISSN/ISBN
0047-2425
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
The objectives of this study were to investigate the impacts of internal nitrification on soil and soil solution acidity and on the rate of nutrient export through NO3- mediated leaching. This was achieved by comparing soil chemical properties and soil solution composition within a naturally N-rich red alder (Alnus rubra Bong.) ecosystem to those of an adjacent Douglas-fir [Pseudotsuga menziesii (Mirbel) Franco] forest where soil N levels were significantly lower and no measurable HNO3 production could be observed. In the red alder system, where > 100 kg ha-1 yr-1 of N were added through symbiotic N2 fixation, the net annual NO3- leaching past the 40-cm soil depth amounted to 3460 mol charges ha-1, and NO3- concentrations in the solutions collected below 40 cm periodically exceeded drinking water standards of 10 mg L-1. The H+ and NO3- release was most pronounced in the forest floor and top 10 cm of the soil under alder occupancy and caused significant acidification of percolating solutions. Less than 1% of the total H+ input from internal (nitrification) and external (atmospheric) sources leached below the 40-cm depth, which was indicative for the strong buffering capacity of this particular soil. The cation displacement reactions involved in this pH buffering caused a 15% decline in base saturation and a significant acidification of the upper part of the soil profile. The presence of large amounts of mobile NO3- in solution triggered accelerated cation leaching, causing a selective redistribution of primarily exchangeable Ca2+ from the A to the B horizon. These field studies lead us to conclude that the rate and the selectivity of NO3- mediated leaching in a red alder system could significantly lower the exchangeable cation pool in the rooting zone or cause nutrient imbalance, if a site is managed for repeated rotations of red alder.
Descriptors
calcium, nitrate, ecology, vascular plant, leaching, nitrogen fixation, nonhuman, pH, plant, priority journal, soil
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Book Title
Database
Embase
Publisher
Data Source
Embase
Authors
Van Miegroet,H., Cole,D. W.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Biodiversity of yeasts, lactic acid bacteria and acetic acid bacteria in the fermentation of "Shanxi aged vinegar", a traditional Chinese vinegar 2012 College of Food Science and Technology, Huazhong Agricultural University, Lion Hill Street No.1, Hongshan District, Wuhan, 430070 Hubei Province, PR China.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Food Microbiology
Periodical, Abbrev.
Food Microbiol.
Pub Date Free Form
May
Volume
30
Issue
1
Start Page
289
Other Pages
297
Notes
LR: 20131121; CI: Copyright (c) 2011; JID: 8601127; 0 (RNA, Ribosomal, 16S); Q40Q9N063P (Acetic Acid); 2011/04/04 [received]; 2011/07/28 [revised]; 2011/08/07 [accepted]; 2011/08/16 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
1095-9998; 0740-0020
Accession Number
PMID: 22265314
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.fm.2011.08.010 [doi]
Output Language
Unknown(0)
PMID
22265314
Abstract
Shanxi aged vinegar is a famous traditional Chinese vinegar made from several kinds of cereal by spontaneous solid-state fermentation techniques. In order to get a comprehensive understanding of culturable microorganism's diversity present in its fermentation, the indigenous microorganisms including 47 yeast isolates, 28 lactic acid bacteria isolates and 58 acetic acid bacteria isolates were recovered in different fermenting time and characterized based on a combination of phenotypic and genotypic approaches including inter-delta/PCR, PCR-RFLP, ERIC/PCR analysis, as well as 16S rRNA and 26S rRNA partial gene sequencing. In the alcoholic fermentation, the dominant yeast species Saccharomyces (S.) cerevisiae (96%) exhibited low phenotypic and genotypic diversity among the isolates, while Lactobacillus (Lb.) fermentum together with Lb. plantarum, Lb. buchneri, Lb. casei, Pediococcus (P.) acidilactici, P. pentosaceus and Weissella confusa were predominated in the bacterial population at the same stage. Acetobacter (A.) pasteurianus showing great variety both in genotypic and phenotypic tests was the dominant species (76%) in the acetic acid fermentation stage, while the other acetic acid bacteria species including A. senegalensis, A. indonesiensis, A. malorum and A. orientalis, as well as Gluconobacter (G.) oxydans were detected at initial point of alcoholic and acetic acid fermentation stage respectively.
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Book Title
Database
Publisher
Elsevier Ltd
Data Source
Authors
Wu,J.J., Ma,Y.K., Zhang,F.F., Chen,F.S.
Original/Translated Title
URL
Date of Electronic
20110816
PMCID
Editors
In situ hydrothermal growth of ytterbium-based metal-organic framework on stainless steel wire for solid-phase microextraction of polycyclic aromatic hydrocarbons from environmental samples 2015 College of Food Science and Engineering, Shandong Agricultural University, Taian, China; Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Analysis and Test Center, Shandong Academy of Sciences, Jinan, Chi
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of chromatography.A
Periodical, Abbrev.
J.Chromatogr.A
Pub Date Free Form
9-Oct
Volume
1415
Issue
Start Page
11
Other Pages
19
Notes
CI: Copyright (c) 2015; JID: 9318488; 0 (Polycyclic Hydrocarbons, Aromatic); 0 (Soil Pollutants); 0 (Waste Water); 0 (Water Pollutants, Chemical); 12597-68-1 (Stainless Steel); MNQ4O4WSI1 (Ytterbium); OTO: NOTNLM; 2015/06/29 [received]; 2015/08/17 [revise
Place of Publication
Netherlands
ISSN/ISBN
1873-3778; 0021-9673
Accession Number
PMID: 26346186
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.chroma.2015.08.036 [doi]
Output Language
Unknown(0)
PMID
26346186
Abstract
In this paper, we report the use of a porous ytterbium-based metal-organic framework (Yb-MOF) coating material with good thermal stability for the headspace solid-phase microextraction (HS-SPME) of polycyclic aromatic hydrocarbons (PAHs) from environmental samples. The Yb-MOF thin films, grown in situ on stainless steel wire in solution, exhibited high selectivity and sensitivity toward PAHs. Under the optimal conditions, the novel fibers achieved large enrichment factors (130-2288), low limits of detection (0.07-1.67ngL(-1)), and wide range of linearity (10-1000ngL(-1)) for 16 PAHs in the tested samples. The novel fiber was successfully used in the analysis of PAHs in real environmental samples. These results demonstrated that Yb-MOF is a promising coating material for the SPME of PAHs at trace levels from environmental samples.
Descriptors
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Book Title
Database
Publisher
Elsevier B.V
Data Source
Authors
Li,Q.L., Wang,X., Chen,X.F., Wang,M.L., Zhao,R.S.
Original/Translated Title
URL
Date of Electronic
20150821
PMCID
Editors
Characterization of Extended-Spectrum beta-Lactamase-Producing Escherichia coli Strains Isolated from Retail Foods in Shaanxi Province, China 2015 College of Food Science and Engineering, Northwest Agriculture-Forest University, Shaanxi 72100, People's Republic of China.; College of Food Science and Engineering, Northwest Agriculture-Forest University, Shaanxi 72100, People's Republic of China.; Col
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of food protection
Periodical, Abbrev.
J.Food Prot.
Pub Date Free Form
May
Volume
78
Issue
5
Start Page
1018
Other Pages
1023
Notes
JID: 7703944; 0 (Anti-Infective Agents); 0 (Bacterial Proteins); 0 (Cephalosporins); EC 3.5.2.- (beta-lactamase CTX-2); EC 3.5.2.- (beta-lactamase TEM-3); EC 3.5.2.6 (beta-Lactamases); ppublish
Place of Publication
United States
ISSN/ISBN
1944-9097; 0362-028X
Accession Number
PMID: 25951400
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.4315/0362-028X.JFP-14-490 [doi]
Output Language
Unknown(0)
PMID
25951400
Abstract
Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains have been reported worldwide; however, the incidence and characterization of foodborne ESBL-producing E. coli strains have been rarely reported in the People's Republic of China. Among a collection of 659 E. coli isolates recovered from retail foods in Shaanxi Province, People's Republic of China, 223 cefoxitin-resistant and/or cefoperazone-resistant isolates were screened for ESBL production with the double disk diffusion test. The ESBL-producing isolates were characterized for antimicrobial resistance and the presence of blaTEM, blaSHV, and blaCTX-M genes. Isolates with blaCTX-M were further classified by PCR as having blaCTX-M-1, blaCTX-M-2, blaCTX-M-8, blaCTX-M-9, or blaCTX-M-25. One hundred forty-seven isolates were identified as ESBL positive. PCR detection revealed that 146 isolates (99.3%) contained the blaCTX-M gene. Among these isolates, 42 (28.8%) were positive for the enzyme CTX-M-1, 5 (3.4%) for CTX-M-2, and 99 (67.8%) for CTX-M-9. No CTX-M-8 and CTX-M-25 were found in this study. One hundred fifteen isolates (78.2%) were positive for the blaTEM gene, but blaSHV was not detected. Among the 147 ESBL-producing E. coli isolates, 75 (51.0%), 35 (23.8%), and 4 (2.7%) isolates were positive for blaTEM and blaCTX-M-9, blaTEM and blaCTX-M-1, and blaTEM and blaCTX-M-2, respectively. All of the 147 ESBL-producing isolates were resistant to three or more non-beta-lactam antibiotics. This study provides evidence that foodborne E. coli can harbor ESBL-encoding genes. Thus, food could be a vehicle for the dissemination of ESBL-producing E. coli strains, a situation that requires surveillance and appropriate management strategies.
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Book Title
Database
Publisher
Data Source
Authors
Xi,M., Wu,Q., Wang,X., Yang,B., Xia,X., Li,D.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Diversity of lactic acid bacteria during fermentation of a traditional Chinese fish product, Chouguiyu (stinky mandarinfish) 2013 College of Food Science and Biotechnology and, Inst. of Aquatic Products Processing, Zhejiang Gongshang Univ., Hangzhou, Zhejiang, 310035, China.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of Food Science
Periodical, Abbrev.
J.Food Sci.
Pub Date Free Form
Nov
Volume
78
Issue
11
Start Page
M1778
Other Pages
83
Notes
CI: (c) 2013; JID: 0014052; 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); OTO: NOTNLM; 2013/05/27 [received]; 2013/09/14 [accepted]; ppublish
Place of Publication
United States
ISSN/ISBN
1750-3841; 0022-1147
Accession Number
PMID: 24245896
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1111/1750-3841.12289 [doi]
Output Language
Unknown(0)
PMID
24245896
Abstract
Chouguiyu, or stinky mandarinfish, is a traditional Chinese fermented fish product made of mandarinfish by spontaneous fermentation at the anaerobic condition with low-salt concentration. In order to get a primary understanding of the microbial community presenting in the Chouguiyu fermentation, 61 cultures of lactic acid bacteria (LAB) from various fermentation period were isolated using MRS agar plates and characterized based on a combination of phenotypic and genotypic approaches including amplified ribosomal DNA restriction analysis (ARDRA) and 16S rRNA partial gene sequencing analysis. Eight distinct bacterial species belonging to 6 genera were identified in total. Among them, Lactobacillus sakei was the dominant species (63%) during the fermentation, which exhibited great variety in phenotypic tests but unique genotypic characters. Meanwhile, the other LAB species including Lactococcus (Lc.) garvieae, Lc. lactis, Lc. raffinolactis, Vagococcus sp., Enterococcus hermanniensis, Macrococcus caseolyticus as well as Streptococcus parauberis were also recovered from the different fermentation periods, especially at the initial point of the fermentation. This seems to be the 1st report investigating the LAB composition involved in Chouguiyu fermentation and the data obtained in this study may be valuable for selecting starter culture for Chouguiyu industrial-scale production.
Descriptors
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Book Title
Database
Publisher
Institute of Food Technologists(R)
Data Source
Authors
Dai,Z., Li,Y., Wu,J., Zhao,Q.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
The spatial distribution of pollutants in pipe-scale of large-diameter pipelines in a drinking water distribution system 2016 College of Engineering and Architecture, Zhejiang University, Hangzhou 310058, China.; College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; Binhai Industrial Technology Research Institute of Zhejiang University, Tia
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of hazardous materials
Periodical, Abbrev.
J.Hazard.Mater.
Pub Date Free Form
5-Nov
Volume
317
Issue
Start Page
27
Other Pages
35
Notes
CI: Copyright (c) 2016; JID: 9422688; OTO: NOTNLM; 2016/02/15 [received]; 2016/05/12 [revised]; 2016/05/14 [accepted]; 2016/05/17 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
1873-3336; 0304-3894
Accession Number
PMID: 27244696
Language
eng
SubFile
Journal Article; IM
DOI
10.1016/j.jhazmat.2016.05.048 [doi]
Output Language
Unknown(0)
PMID
27244696
Abstract
In large-diameter drinking water pipelines, spatial differences in hydraulic and physiochemical conditions may also result in spatial variations in pipe corrosion, biofilm growth and pollutant accumulation. In this article, the spatial distributions of various metals and organic contaminants in two 19-year-old grey cast iron pipes which had an internal diameter of 600mm (DN600), were investigated and analyzed by Atomic Absorption Spectrometry, Gas Chromatography-Mass Spectrometry, Energy Dispersive Spectrometer, X-ray Diffraction, etc. The spatial distribution of heavy metals varied significantly across the pipe section, and iron, manganese, lead, copper, and chromium were highest in concentration in the upper portion pipe-scales. However, the highest aluminum and zinc content was detected in the lower portion pipe-scales. Apart from some common types of hydrocarbons formed by microbial metabolites, there were also some microalgae metabolites and exogenous contaminants accumulated in pipe-scale, which also exhibited high diversity between different spatial locations. The spatial distributions of the physical and chemical properties of pipe-scale and contaminants were quite different in large-diameter pipes. The finding put forward higher requirements on the research method about drinking water distribution system chemical safety. And the scientific community need understand trend and dynamics of drinking water pipe systems better.
Descriptors
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Book Title
Database
Publisher
Elsevier B.V
Data Source
Authors
Liu,J., Chen,H., Yao,L., Wei,Z., Lou,L., Shan,Y., Endalkachew,S.D., Mallikarjuna,N., Hu,B., Zhou,X.
Original/Translated Title
URL
Date of Electronic
20160517
PMCID
Editors
Effect of altering the type of enamel conditioner on the shear bond strength of a resin-reinforced glass ionomer adhesive 2000 College of Dentistry, University of Iowa, Iowa City, IA 52242-1001, USA.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, its Constituent Societies, and the American Board of Orthodontics
Periodical, Abbrev.
Am.J.Orthod.Dentofacial Orthop.
Pub Date Free Form
Sep
Volume
118
Issue
3
Start Page
288
Other Pages
294
Notes
LR: 20131121; JID: 8610224; 0 (Acrylic Resins); 0 (Aluminum Silicates); 0 (Dentin-Bonding Agents); 0 (Fuji Ortho LC); 0 (Glass Ionomer Cements); 0 (Phosphoric Acids); 0 (Resin Cements); 135669-49-7 (Transbond); 454I75YXY0 (Bisphenol A-Glycidyl Methacrylat
Place of Publication
UNITED STATES
ISSN/ISBN
0889-5406; 0889-5406
Accession Number
PMID: 10982929
Language
eng
SubFile
Comparative Study; Journal Article; D; IM
DOI
S0889-5406(00)66280-7 [pii]
Output Language
Unknown(0)
PMID
10982929
Abstract
The purpose of this study was to determine the effects of changing the type of enamel conditioner on the shear bond strength of a resin-reinforced glass ionomer within half an hour after bonding the bracket to the tooth. Freshly extracted human molars were collected and stored in a solution of 0.1% (weight/volume) thymol. The teeth were cleaned and polished. The teeth were randomly separated into 4 groups according to the enamel conditioner/etchant and adhesive used: group I, teeth were conditioned with 10% polyacrylic acid and brackets were bonded with a resin-reinforced glass ionomer adhesive; group II, teeth were conditioned with 20% polyacrylic acid and brackets were bonded with a resin-reinforced glass ionomer adhesive; group III, teeth were etched with 37% phosphoric acid and the brackets were bonded with a resin-reinforced glass ionomer adhesive; group IV, teeth were etched with 37% phosphoric acid and the brackets were bonded with a composite adhesive. The results of the analysis of variance comparing the 4 experimental groups (F = 24.87) indicated the presence of significant differences between the groups (P =.0001). In general, the shear bond strengths were significantly greater in the 2 groups etched with 37% phosphoric acid. This was true for both the resin-reinforced glass ionomer (X = 6.1 +/- 2.7 MPa) and the composite (X = 5.2 +/- 2.9 MPa) adhesives. On the other hand, the shear bond strengths were significantly lower in the two groups conditioned with polyacrylic acid. The bond strength of the resin-reinforced glass ionomer adhesive conditioned with 10% polyacrylic acid (X = 0.4 +/- 1.0 MPa) was significantly lower than the group conditioned with 20% polyacrylic acid (&xmacr; = 3.3 +/- 2.6 MPa). The present findings indicated that the bond strength of the resin-reinforced glass ionomer adhesive can be significantly increased in the initial half hour after bonding if the enamel is etched with 37% phosphoric acid instead of being conditioned with either 10% or 20% polyacrylic acid. The clinician needs to take these properties into consideration when ligating the initial archwires.
Descriptors
Acid Etching, Dental/methods, Acrylic Resins, Aluminum Silicates, Analysis of Variance, Bisphenol A-Glycidyl Methacrylate, Chi-Square Distribution, Dental Bonding, Dental Enamel/drug effects, Dental Stress Analysis, Dentin-Bonding Agents, Glass Ionomer Cements, Humans, Orthodontic Brackets, Phosphoric Acids, Random Allocation, Resin Cements, Statistics, Nonparametric, Tensile Strength
Links
Book Title
Database
Publisher
Data Source
Authors
Bishara,S. E., Vonwald,L., Laffoon,J. F., Jakobsen,J. R.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors