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Pilot Study of Waterpipe Tobacco Smoking Among US Muslim College Students 2015 Department of Psychiatry and Behavioral Neurosciences, Wayne State University, 3901 Chrysler Service Drive, Ste 1B, Rm 156, Detroit, MI, 48201, USA, carfken@med.wayne.edu.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of religion and health
Periodical, Abbrev.
J.Relig.Health.
Pub Date Free Form
Oct
Volume
54
Issue
5
Start Page
1543
Other Pages
1554
Notes
JID: 2985199R; ppublish
Place of Publication
United States
ISSN/ISBN
1573-6571; 0022-4197
Accession Number
PMID: 24797155
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1007/s10943-014-9871-x [doi]
Output Language
Unknown(0)
PMID
24797155
Abstract
Waterpipe smoking is common among the young in Muslim-majority countries despite recent Islamic rulings on tobacco. US Muslim college students, especially immigrants, may be at high risk for smoking, but information is lacking. In this pilot study, respondent-driven sampling was used to sample 156 Muslim college students. Waterpipe smoking was common (44.3%). Leading motivations to smoke were social and perceived low tobacco harm. Independent risk factors among the Muslim students were perception that friends and other students smoked, and ever drank alcohol. Personal belief that waterpipe smoking is prohibited in Islam was not significant. This pilot suggests that Muslim students are at high risk for waterpipe smoking and more definitive studies are needed.
Descriptors
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Book Title
Database
Publisher
Data Source
Authors
Arfken,C.L., Abu-Ras,W., Ahmed,S.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
The association between senior student tobacco use rate at school and alternative tobacco product use among junior students in Canadian secondary schools 2014 School of Public Health and Health Systems, University of Waterloo, 200 University Avenue, Waterloo, ON N2L 3G1, Canada.; School of Public Health and Health Systems, University of Waterloo, 200 University Avenue, Waterloo, ON N2L 3G1, Canada.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Tobacco induced diseases
Periodical, Abbrev.
Tob Induc Dis.
Pub Date Free Form
1-May
Volume
12
Issue
1
Start Page
8
Other Pages
9625-12-8. eCollection 2014
Notes
LR: 20140512; JID: 101201591; OID: NLM: PMC4012246; OTO: NOTNLM; 2014 [ecollection]; 2014/04/02 [received]; 2014/04/25 [accepted]; 2014/05/01 [epublish]; epublish
Place of Publication
England
ISSN/ISBN
2070-7266; 1617-9625
Accession Number
PMID: 24808817
Language
eng
SubFile
Journal Article
DOI
10.1186/1617-9625-12-8 [doi]
Output Language
Unknown(0)
PMID
24808817
Abstract
BACKGROUND: The use of alternative tobacco products (ATPs) has grown in popularity among Canadian youth. This study examined the association between a school-level characteristic (the senior student tobacco use rate) and the current use of manufactured cigarettes, little cigars or cigarillos, cigars, roll-your-own cigarettes, smokeless tobacco (SLT), and a hookah among junior students. METHODS: This study used nationally representative Canadian data from 29,495 students in grades 9 to 12 as part of the 2010/2011 Youth Smoking Survey. For each ATP, we described rates of senior and junior tobacco use, calculated the variance attributed to school-level factors, and examined the association between the senior student (grades 11 and 12) tobacco use rate and the current use of each ATP among junior students (grades 9 and 10) while accounting for relevant student-level characteristics. SAS 9.3 was used for all analyses. RESULTS: Over half of schools sampled had senior students that reported using each ATP. School-level differences accounted for between 14.1% and 29.7% of the variability in ATP current use among junior students. Each one percent increase in the number of senior students at a school that currently use manufactured cigarettes, SLT, or a hookah was significantly independently associated with an increased likelihood that a junior student at that school currently used manufactured cigarettes (OR 1.04, 95% CI 1.01 to 1.06), SLT (OR 1.14, 95% CI 1.06 to 1.24), or a hookah (OR 1.09, 95% CI 1.03 to 1.14). CONCLUSIONS: Characteristics of the school environment a junior student attends appear to play an important role in ATP use, and tobacco control programs and policies should be designed to ensure that they include strategies to curb the use of all tobacco products. Additional evidence is needed for the impact of comprehensive school-based tobacco control approaches.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Cole,A.G., Leatherdale,S.T.
Original/Translated Title
URL
Date of Electronic
20140501
PMCID
PMC4012246
Editors
Pre- and postnatal exposure to tobacco smoke and respiratory outcomes during the first year 2015 Unit of Addictive Disorders, San Marcelino Primary Health Care Center, Valencia, Spain; Center for Public Health Research (CSISP/FISABIO), Valencia, Spain; Spanish Consortium for Research on Epidemiology and Public Health (CIBERESP), Madrid, Spain.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Indoor air
Periodical, Abbrev.
Indoor Air
Pub Date Free Form
Feb
Volume
25
Issue
1
Start Page
4
Other Pages
12
Notes
CI: (c) 2014; JID: 9423515; 0 (Tobacco Smoke Pollution); OTO: NOTNLM; 2014/01/17 [received]; 2014/05/02 [accepted]; 2014/06/21 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
1600-0668; 0905-6947
Accession Number
PMID: 24810295
Language
eng
SubFile
Journal Article; Multicenter Study; IM
DOI
10.1111/ina.12128 [doi]
Output Language
Unknown(0)
PMID
24810295
Abstract
The different role of prenatal and postnatal exposure to tobacco smoke in respiratory outcomes in infants has not yet been clearly established. Our objective is to assess the effects of these exposures on the risk of respiratory outcomes during the first year of life of infants from a Spanish multicenter cohort study. A total of 2506 women were monitored until delivery. About 2039 infants made up the final population. The outcomes were caused by the occurrence of the following: otitis, cough persisting for more than 3 weeks, lower respiratory tract symptoms (wheezing or chestiness), and lower respiratory tract infections (bronchitis, bronchiolitis, or pneumonia). The relationship between prenatal and postnatal exposure and health outcomes was explored using logistic regression analysis. Maternal smoking during pregnancy increased the odds for wheezing (OR: 1.41, 95% CI: 0.99-2.01) and chestiness (OR: 1.46, 95% CI: 1.03-2.01). Postnatal exposure from fathers was associated with otitis (OR: 1.25, 95% CI: 1.01-1.54). Passive exposure at work of non-smoking mothers during pregnancy was related to cough (OR: 1.62, 95% CI: 1.05-2.51). Exposure to tobacco smoke was related to a higher risk of experiencing respiratory outcomes in young infants. Prenatal exposure was that most clearly associated with the respiratory outcomes analyzed.
Descriptors
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Book Title
Database
Publisher
John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Data Source
Authors
Fuentes-Leonarte,V., Estarlich,M., Ballester,F., Murcia,M., Esplugues,A., Aurrekoetxea,J.J., Basterrechea,M., Fernandez-Somoano,A., Morales,E., Gascon,M., Tardon,A., Rebagliato,M.
Original/Translated Title
URL
Date of Electronic
20140621
PMCID
Editors
Exposure to secondhand smoke and attitudes toward smoke-free workplaces among employed U.S. adults: findings from the National Adult Tobacco Survey 2014 Office on Smoking and Health, National Center for Chronic Disease Prevention and Health Promotion, Centers for Disease Control and Prevention, Atlanta, GA baking@cdc.gov.; Office on Smoking and Health, National Center for Chronic Disease Prevention and He
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco
Periodical, Abbrev.
Nicotine Tob.Res.
Pub Date Free Form
Oct
Volume
16
Issue
10
Start Page
1307
Other Pages
1318
Notes
LR: 20151026; CI: Published by Oxford University Press on behalf of the Society for Research on Nicotine and Tobacco 2014; GR: CC999999/Intramural CDC HHS/United States; JID: 9815751; 0 (Tobacco Smoke Pollution); HHSPA709982; OID: NLM: HHSPA709982; OID: N
Place of Publication
England
ISSN/ISBN
1469-994X; 1462-2203
Accession Number
PMID: 24812025
Language
eng
SubFile
Journal Article; IM
DOI
10.1093/ntr/ntu069 [doi]
Output Language
Unknown(0)
PMID
24812025
Abstract
INTRODUCTION: This study assessed the prevalence and correlates of secondhand smoke (SHS) exposure and attitudes toward smoke-free workplaces among employed U.S. adults. METHODS: Data came from the 2009-2010 National Adult Tobacco Survey, a landline and cellular telephone survey of adults aged >/=18 years in the United States and the District of Columbia. National and state estimates of past 7-day workplace SHS exposure and attitudes toward indoor and outdoor smoke-free workplaces were assessed among employed adults. National estimates were calculated by sex, age, race/ethnicity, education, annual household income, sexual orientation, U.S. region, and smoking status. RESULTS: Among employed adults who did not smoke cigarettes, 20.4% reported past 7-day SHS exposure at their workplace (state range: 12.4% [Maine] to 30.8% [Nevada]). Nationally, prevalence of exposure was higher among males, those aged 18-44 years, non-Hispanic Blacks, Hispanics, and non-Hispanic American Indians/Alaska natives compared to non-Hispanic Whites, those with less education and income, those in the western United States, and those with no smoke-free workplace policy. Among all employed adults, 83.8% and 23.2% believed smoking should never be allowed in indoor and outdoor areas of workplaces, respectively. CONCLUSIONS: One-fifth of employed U.S. adult nonsmokers are exposed to SHS in the workplace, and disparities in exposure exist across states and subpopulations. Most employed adults believe indoor areas of workplaces should be smoke free, and nearly one-quarter believe outdoor areas should be smoke free. Efforts to protect employees from SHS exposure and to educate the public about the dangers of SHS and benefits of smoke-free workplaces could be beneficial.
Descriptors
Links
Book Title
Database
Publisher
. This work is written by (a) US Government employee(s) and is in the public domain in the US
Data Source
Authors
King,B.A., Homa,D.M., Dube,S.R., Babb,S.D.
Original/Translated Title
URL
Date of Electronic
20140508
PMCID
PMC4517583
Editors
Substance abuse in relation to religiosity and familial support in Iranian college students 2014 Department of Public Health, Qazvin University of Medical Sciences, Qazvin, Iran. Electronic address: poorasl@yahoo.com.; Department of Epidemiology and Biostatistics, Tehran University of Medical Science, Tehran, Iran. Electronic address: abbasi.abbas49@
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Asian journal of psychiatry
Periodical, Abbrev.
Asian J.Psychiatr.
Pub Date Free Form
Jun
Volume
9
Issue
Start Page
41
Other Pages
44
Notes
LR: 20160519; CI: Copyright (c) 2014; JID: 101517820; OTO: NOTNLM; 2013/02/21 [received]; 2013/12/26 [revised]; 2013/12/29 [accepted]; 2014/01/07 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
1876-2026; 1876-2018
Accession Number
PMID: 24813035
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.ajp.2013.12.015 [doi]
Output Language
Unknown(0)
PMID
24813035
Abstract
BACKGROUND AND OBJECTIVES: Drug abuse and alcohol use in young adults are a major public health problem. The aims of present study were to determine the prevalence of alcohol use and substance abuse and related factors in a sample of Iranian college students. METHODS: The study took place in Tabriz (northwest of Iran) in April and May, 2011. The randomly selected sample consisted of 1837 college students. Data were collected in a survey. A self-administered questionnaire was used to measure smoking, sexual behavior, alcohol and drug use, religious belief, and parental support. RESULTS: The prevalence of alcohol use in the past 30 days and ever drug abuse were 7.7% and 8.0%, respectively. After adjusting for other factors, living in dormitory in comparison to parental home (OR=0.61) and having higher score of religious beliefs (OR=0.98) were protective factors for ever use of illicit drugs. Being male (OR=1.89), living in the single house in comparison to parental home (OR=2.64), smoking (OR=2.0), alcohol use (OR=4.71), hookah smoking (OR=2.89), and having unsafe sex (OR=2.40) were risk factors ever use of illicit drugs. CONCLUSION: Our results show low prevalence of alcohol use and drug abuse in Iranian college students and determined some of its associated factors. The findings of this research can be used for planning and evaluating interventions by considering risk and protective factors.
Descriptors
Links
Book Title
Database
Publisher
Elsevier B.V
Data Source
Authors
Mohammadpoorasl,A., Ghahramanloo,A.A., Allahverdipour,H., Augner,C.
Original/Translated Title
URL
Date of Electronic
20140107
PMCID
Editors
Occupational secondhand smoke is the main determinant of hair nicotine concentrations in bar and restaurant workers 2014 School of Public Health, Faculty of Medicine, University of Chile, Chile. Electronic address: viglesia@med.uchile.cl.; Faculty of Medicine, University of Chile, Chile.; Laboratory of Occupational Health, Institute of Public Health, Chile. Electronic addre
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Environmental research
Periodical, Abbrev.
Environ.Res.
Pub Date Free Form
Jul
Volume
132
Issue
Start Page
206
Other Pages
211
Notes
LR: 20151119; CI: Copyright (c) 2014; GR: D43 TW005746/TW/FIC NIH HHS/United States; GR: D43TW005746-02/TW/FIC NIH HHS/United States; JID: 0147621; 0 (Tobacco Smoke Pollution); 6M3C89ZY6R (Nicotine); NIHMS666789; OID: NLM: NIHMS666789; OID: NLM: PMC435199
Place of Publication
United States
ISSN/ISBN
1096-0953; 0013-9351
Accession Number
PMID: 24813578
Language
eng
SubFile
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.envres.2014.03.044 [doi]
Output Language
Unknown(0)
PMID
24813578
Abstract
OBJECTIVE: To evaluate the relative contribution of occupational vs. non-occupational secondhand tobacco smoke exposure to overall hair nicotine concentrations in non-smoking bar and restaurant employees. METHOD: We recruited 76 non-smoking employees from venues that allowed smoking (n=9), had mixed policies (smoking and non-smoking areas, n=13) or were smoke-free (n=2) between April and August 2008 in Santiago, Chile. Employees used personal air nicotine samplers during working and non-working hours for a 24-h period to assess occupational vs. non-occupational secondhand tobacco smoke exposure and hair nicotine concentrations to assess overall secondhand tobacco smoke exposure. RESULTS: Median hair nicotine concentrations were 1.5 ng/mg, interquartile range (IQR) 0.7 to 5.2 ng/mg. Time weighted average personal air nicotine concentrations were higher during working hours (median 9.7, IQR 3.3-25.4 microg/m(3)) compared to non-working hours (1.7, 1.0-3.1 microg/m(3)). Hair nicotine concentration was best predicted by personal air nicotine concentration at working hours. After adjustment, a 2-fold increase in personal air nicotine concentration in working hours was associated with a 42% increase in hair nicotine concentration (95% confidence interval 14-70%). Hair nicotine concentration was not associated with personal air nicotine concentration during non-working hours (non-occupational exposure). CONCLUSIONS: Personal air nicotine concentration at working hours was the major determinant of hair nicotine concentrations in non-smoking employees from Santiago, Chile. Secondhand tobacco smoke exposure during working hours is a health hazard for hospitality employees working in venues where smoking is allowed.
Descriptors
Links
Book Title
Database
Publisher
Elsevier Inc
Data Source
Authors
Iglesias,V., Erazo,M., Droppelmann,A., Steenland,K., Aceituno,P., Orellana,C., Acuna,M., Peruga,A., Breysse,P.N., Navas-Acien,A.
Original/Translated Title
URL
Date of Electronic
20140508
PMCID
PMC4351991
Editors
Relationship between increases in BMI and changes in periodontal status: a prospective cohort study 2014 Departments of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of clinical periodontology
Periodical, Abbrev.
J.Clin.Periodontol.
Pub Date Free Form
Aug
Volume
41
Issue
8
Start Page
772
Other Pages
778
Notes
CI: (c) 2014; JID: 0425123; OTO: NOTNLM; 2014/05/03 [accepted]; 2014/06/30 [aheadofprint]; ppublish
Place of Publication
United States
ISSN/ISBN
1600-051X; 0303-6979
Accession Number
PMID: 24813869
Language
eng
SubFile
Journal Article; D; IM
DOI
10.1111/jcpe.12273 [doi]
Output Language
Unknown(0)
PMID
24813869
Abstract
AIM: The purpose of this prospective cohort study was to investigate whether body mass index (BMI) and oral health behaviour are related to changes in periodontal status in Japanese university students. MATERIALS AND METHODS: Students (n = 224) who were interested in receiving oral health examinations before entering university and before graduation were included in the analysis. Subjects were investigated regarding the correlations of oral health behaviours and increases in BMI with the percentage of bleeding on probing (%BOP) and Community Periodontal Index (CPI) scores as indicators of changes in periodontal status. RESULTS: The risk of increased %BOP was associated with the non-use of dental floss (adjusted odds ratio [OR]: 3.11; 95% confidence interval [CI]: 1.31-7.37; p
Descriptors
Links
Book Title
Database
Publisher
John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Data Source
Authors
Ekuni,D., Mizutani,S., Kojima,A., Tomofuji,T., Irie,K., Azuma,T., Yoneda,T., Furuta,M., Eshima,N., Iwasaki,Y., Morita,M.
Original/Translated Title
URL
Date of Electronic
20140630
PMCID
Editors
The changes in smoking related behaviours and second hand smoke after the smoking ban in Izmir 2014 Department of Chest Diseases, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey. onurtura@yahoo.com.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Tuberkuloz ve toraks
Periodical, Abbrev.
Tuberk.Toraks
Pub Date Free Form
Volume
62
Issue
1
Start Page
27
Other Pages
38
Notes
LR: 20151119; JID: 0417364; 0 (Tobacco Smoke Pollution); ppublish
Place of Publication
Turkey
ISSN/ISBN
0494-1373; 0494-1373
Accession Number
PMID: 24814075
Language
tur
SubFile
English Abstract; Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
24814075
Abstract
INTRODUCTION: The aim of the study is to determine the views about law, change of second hand smoke (SHS) quantity, people's smoking related behaviours after the publishment of law which prohibits use of tobacco products in closed public areas. MATERIALS AND METHODS: Questionnaire including 47 questions was performed to 600 people, who were selected by Turkey Statistics Instutition to present population of Izmir. RESULTS: 53.7% of participants were female and 46.3% was male; the mean age was 41.5 +/- 15.6. 98% of all participants were aware of the law and 91.5% of them were supporting it. 42% of participants who succeeded in quitting had managed it after the ban. Restriction of areas, rise of prices and effects of SHS were the most popular reasons of reducing/quitting smoking after the law. Non-smokers were exposed to SHS at homes; 35.1%, at vehicles: 14.9%, at work places: 14.4%. The number of smoking cigarettes was reduced by 3.19 cigarettes/day. The rate of violation of the ban was 32.3%; only 21.3% of violators were warned. 96.4% of law supporters found law successful and sufficient. CONCLUSION: A big amount of participants thinks about quitting in Izmir; law is thought to have important effect on this decision. SHS has reduced mostly in public areas with ban, but law must be enlarged to prevent SHS at private areas such as houses, vehicles and workplaces. As a result; the ban encouraged smokers to quit and reduced SHS at public areas. We believe that paying more attention for law sanction and extending the borders of law and reviewing the ban, will make our country more smoke free from now.
Descriptors
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Database
Publisher
Data Source
Authors
Turan,P.A., Ergor,G., Turan,O., Doganay,S., Kilinc,O.
Original/Translated Title
4207 sayili yasanin yururluge girmesinden sonra sigara icme davranislari ve pasif maruziyet ile ilgili degisikliklerin saptanmasi
URL
Date of Electronic
PMCID
Editors
Lifetime cumulative exposure to waterpipe smoking is associated with coronary artery disease 2014 Department of Epidemiology & Population Health, Faculty of Health Sciences, American University of Beirut, Beirut, Lebanon.; Department of Epidemiology & Population Health, Faculty of Health Sciences, American University of Beirut, Beirut, Lebanon; Center
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Atherosclerosis
Periodical, Abbrev.
Atherosclerosis
Pub Date Free Form
Jun
Volume
234
Issue
2
Start Page
454
Other Pages
460
Notes
CI: Copyright (c) 2014; JID: 0242543; OTO: NOTNLM; 2013/11/22 [received]; 2014/03/20 [revised]; 2014/03/31 [accepted]; 2014/04/15 [aheadofprint]; ppublish
Place of Publication
Ireland
ISSN/ISBN
1879-1484; 0021-9150
Accession Number
PMID: 24814409
Language
eng
SubFile
Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.atherosclerosis.2014.03.036 [doi]
Output Language
Unknown(0)
PMID
24814409
Abstract
OBJECTIVE: Globally, waterpipe (WP) smoking is becoming a more prevalent form of tobacco consumption. Whilst research so far has demonstrated a significant link between WP use and a number of health outcomes, little is known of its association with heart disease. We examine in this study the association of WP smoking with angiographically confirmed coronary artery disease (CAD). METHODS: A total of 1210 patients, aged 40 years and over and free from smoking-associated illnesses or history of cardiovascular procedures, admitted for coronary angiography at four major hospitals in Lebanon, were included. The extent of CAD was summarized in two ways, firstly as diseased (>/= 50% and >/= 70% occlusion in at least one main coronary artery) versus non-diseased (entirely normal coronaries), and secondly, as CAD cumulative score based on Duke CAD Prognostic Index. A score of WP-years, capturing intensity and lifetime duration of exposure, was estimated for each individual. RESULTS: Lifetime exposure exceeding 40 WP-years was associated with a threefold significant increase in the odds of having severe stenosis (>/= 70%) compared to non-smokers (OR = 2.94, 95% CI 1.04-8.33) as well as with the CAD Index (beta = 7.835, p-value = 0.027), net of the effect of socio-demographic characteristics, health behaviors and co-morbidity. A dose-response relationship between WP-years and percent stenosis was also established. WP smoking status (never, past and current) did not associate with CAD. CONCLUSIONS: Cumulative exposure to WP smoking is significantly associated with severe CAD. There is a need to monitor WP use among cardiac patients and include this information in their medical charts in the same manner cigarettes smoking is documented. This is likely to increase awareness of the hazards of WP smoking and prompt physicians to target WP tobacco control by providing advice to their patients on WP smoking cessation.
Descriptors
Links
Book Title
Database
Publisher
Elsevier Ireland Ltd
Data Source
Authors
Sibai,A.M., Tohme,R.A., Almedawar,M.M., Itani,T., Yassine,S.I., Nohra,E.A., Isma'eel,H.A.
Original/Translated Title
URL
Date of Electronic
20140415
PMCID
Editors
Chromosomal location of blaCTX-M genes in clinical isolates of Escherichia coli from Germany, The Netherlands and the UK 2014 Federal Institute for Risk Assessment (BfR), Department for Biological Safety, Max-Dohrn Strasse 8-10, D-10589 Berlin, Germany. Electronic address: irodriguezf@salud.madrid.org.; Federal Institute for Risk Assessment (BfR), Department for Biological Safet
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International journal of antimicrobial agents
Periodical, Abbrev.
Int.J.Antimicrob.Agents
Pub Date Free Form
Jun
Volume
43
Issue
6
Start Page
553
Other Pages
557
Notes
CI: Copyright (c) 2014; JID: 9111860; 0 (Cephalosporins); 0 (DNA, Bacterial); EC 3.5.2.6 (beta-Lactamases); OTO: NOTNLM; 2013/10/21 [received]; 2014/02/26 [revised]; 2014/02/26 [accepted]; 2014/04/16 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
1872-7913; 0924-8579
Accession Number
PMID: 24816185
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.ijantimicag.2014.02.019 [doi]
Output Language
Unknown(0)
PMID
24816185
Abstract
This study aimed to detect and characterise clinical Escherichia coli isolates suspected of carrying chromosomally encoded CTX-M enzymes. Escherichia coli (n=356) obtained in Germany, The Netherlands and the UK (2005-2009) and resistant to third-generation cephalosporins were analysed for the presence of ESBL-/AmpC-encoding genes within the European SAFEFOODERA-ESBL project. beta-Lactamases and their association with IS26 and ISEcp1 were investigated by PCR. Isolates were typed by phylogenetic grouping, MLST and PFGE. Plasmids were visualised by S1 nuclease PFGE, and the location of blaCTX-M genes was determined by Southern hybridisation of XbaI-, S1- and I-CeuI-digested DNA. ESBL enzymes could not be located on plasmids in 17/356 isolates (4.8%). These 17 isolates, from different countries and years, were ascribed to phylogenetic groups D (9), B2 (6) and B1 (2), and to seven sequence types, with ST38 being the most frequent (7 phylogroup D isolates). Eleven isolates produced CTX-M-15. blaCTX-M-15 genes were associated with ISEcp1. The remaining isolates expressed the CTX-M group 9 beta-lactamases CTX-M-14 (4), CTX-M-9 (1) and CTX-M-51 (1). blaCTX-M probes hybridised with I-CeuI- and/or XbaI-digested DNA, but not with S1-digested DNA, corroborating their chromosomal location. To summarise, only 4.8% of a large collection of ESBL-producing E. coli isolates harboured chromosomal blaCTX-M genes. These isolates were of human origin and belonged predominantly to ST38 and ST131, which possibly indicates the role of these sequence types in this phenomenon. However, heterogeneity among isolates was found, suggesting that their spread is not only due to the dispersion of successful E. coli clones.
Descriptors
Links
Book Title
Database
Publisher
Elsevier B.V. and the International Society of Chemotherapy
Data Source
Authors
Rodriguez,I., Thomas,K., Van Essen,A., Schink,A.K., Day,M., Chattaway,M., Wu,G., Mevius,D., Helmuth,R., Guerra,B., SAFEFOODERA-ESBL consortium
Original/Translated Title
URL
Date of Electronic
20140416
PMCID
Editors