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Cold tap water as a source of fatal nosocomial pneumonia due to Legionella pneumophila in a rehabilitation center 1999 Gemeenschappelijke Gezondheidsdienst Oostelijk Zuid-Limburg, Heerlen. choebe@knmg.nl
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Journal Article
Periodical, Full
Nederlands tijdschrift voor geneeskunde
Periodical, Abbrev.
Ned.Tijdschr.Geneeskd.
Pub Date Free Form
15-May
Volume
143
Issue
20
Start Page
1041
Other Pages
1045
Notes
LR: 20081121; JID: 0400770; ppublish
Place of Publication
NETHERLANDS
ISSN/ISBN
0028-2162; 0028-2162
Accession Number
PMID: 10368731
Language
dut
SubFile
Case Reports; English Abstract; Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
10368731
Abstract
OBJECTIVE: Report of the technical, microbiological and epidemiological investigation following 2 cases of fatal Legionella pneumonia. DESIGN: Descriptive. METHOD: Faced with 2 nosocomial cases in a rehabilitation centre in the South of Limburg, the Netherlands, the water supply was investigated. Water temperatures from different taps were measured. Legionella cultures were made from respiratory patients' specimens, water samples and smears from all mixing taps (used in showers), samples from hot and cold water taps from the infected ward and from the five other wards. The strains were typed by serotyping and polymerase chain reaction. RESULTS: The circulating cold water sometimes warmed up to 40 degrees C (within the Legionella growth range). From the sputum of the 2 male patients with rheumatoid arthritis who died of Legionella pneumonia the same Legionella pneumophila (serotype I) was cultured as from the water supply. Of the showers on the contaminated ward 19% (12/63) were positive for Legionella as were 59% (35/59) of the cold water taps. Cultures from the hot water supply were negative just like control cultures from five other wards and swabs from showerheads and hoses. The cold water tubes ran next to the hot water tubes and the central heating system in the same shaft. On the infected ward patients were absent during the weekends. As one of the subsequent measures, the cold water pipes were relocated to another shaft. CONCLUSION: The combination of an elevated cold water temperature caused by heating along a distance by nearby hot water and heating piping and the regular stasis of water during the weekends when the ward was closed, most probably stimulated the multiplication of Legionella in the water supply. In order to minimize contamination of cold water its temperature must be kept below 20 degrees C. Surveillance of intramural water systems is necessary to prevent nosocomial infections.
Descriptors
Cold Temperature, Cross Infection/microbiology/prevention & control, Disease Reservoirs, Fatal Outcome, Humans, Legionella pneumophila/isolation & purification, Legionnaires' Disease/complications/microbiology/prevention & control, Male, Netherlands, Pneumonia/microbiology/prevention & control, Polymerase Chain Reaction, Rehabilitation Centers, Serotyping, Water Microbiology, Water Supply/analysis
Links
Book Title
Database
Publisher
Data Source
Authors
Hoebe,C. J., Cluitmans,J. J., Wagenvoort,J. H., van Leeuwen,W. J., Bilkert-Mooiman,M. A.
Original/Translated Title
Koud leidingwater als bron van fatale nosocomiale pneumonieen door Legionella pneumophila in een revalidatiekliniek
URL
Date of Electronic
PMCID
Editors
The effects of water pipe smoking on hematological parameters in rats 2014 Genetics of Non-Communicable Disease Research Centre, Zahedan University of Medical Sciences, Zahedan, Iran ; Department of Genetics, Zahedan University of Medical Sciences, Zahedan, Iran.; Health promotion research Center, Faculty of Health, Zahedan Univ
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Print(0)
Ref Type
Journal Article
Periodical, Full
International journal of hematology-oncology and stem cell research
Periodical, Abbrev.
Int.J.Hematol.Oncol.Stem Cell.Res.
Pub Date Free Form
1-Jul
Volume
8
Issue
3
Start Page
37
Other Pages
43
Notes
LR: 20150204; JID: 101511150; OID: NLM: PMC4305380; OTO: NOTNLM; 2014/01/16 [received]; 2014/04/23 [accepted]; ppublish
Place of Publication
Iran
ISSN/ISBN
2008-3009; 2008-2207
Accession Number
PMID: 25642307
Language
eng
SubFile
Journal Article
DOI
Output Language
Unknown(0)
PMID
25642307
Abstract
OBJECTIVES: This study aimed to investigate the effect of water-pipe (WP) smoking on hematological parameters of Wistar rats. METHODS: Thirty-five young male rats (200-250 g) were randomly assigned to five groups (n=7). The control group was exposed to room air and the experimental groups were exposed to WP smoking, using a special apparatus designed to have the ability to keep the rats for 40 minutes every day for 4, 8, 12 and 12 weeks; moreover, one of the two groups of 12 weeks of WP exposer had four following weeks of rest. Blood samples were collected to evaluate red blood cell count, hemoglobin, hematocrit, white blood cell and platelet counts. RESULTS: The results showed that RBC count, Hb and Hct parameters were significantly higher in WP smoking rats than the control group (P
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Miri-Moghaddam,E., Mirzaei,R., Arab,M.R., Kaikha,S.
Original/Translated Title
URL
Date of Electronic
PMCID
PMC4305380
Editors
Reliability of internet- versus telephone-administered questionnaires in a diverse sample of smokers 2008 Georgetown University Medical Center / Lombardi Comprehensive Cancer Center, 3300 Whitehaven St, NW, Milton Harris Bldg, Suite 4100, Washington, DC 20007, USA. alg45@georgetown.edu
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of medical Internet research
Periodical, Abbrev.
J.Med.Internet Res.
Pub Date Free Form
26-Mar
Volume
10
Issue
1
Start Page
e8
Other Pages
Notes
LR: 20151119; ClinicalTrials.gov/NCT00282009; GR: R01 CA104836/CA/NCI NIH HHS/United States; GR: R01 CA104836-05/CA/NCI NIH HHS/United States; JID: 100959882; OID: NLM: PMC2483847; 2007/10/28 [received]; 2008/02/20 [accepted]; 2008/02/07 [revised]; epubli
Place of Publication
United States
ISSN/ISBN
1438-8871; 1438-8871
Accession Number
PMID: 18364345
Language
eng
SubFile
Journal Article; Randomized Controlled Trial; IM
DOI
10.2196/jmir.987 [doi]
Output Language
Unknown(0)
PMID
18364345
Abstract
BACKGROUND: Smoking is more prevalent among lower-income individuals and certain racial/ethnic minorities. Addressing tobacco cessation among diverse populations is an urgent public health priority. As Internet use continues to rise among all segments of the US population, Web-based interventions have enormous potential to reach priority populations. Conducting Web-based smoking cessation research in priority populations requires psychometrically sound measurement instruments. To date, only one published study has examined the psychometric properties of Internet-administered measures commonly used in Web-based cessation trials. However, the sample was homogeneous with regard to race/ethnicity and income. We sought to replicate and extend these findings in a more diverse sample of smokers. OBJECTIVE: The aim was to examine the internal consistency and test-retest reliability of measures commonly used in smoking cessation clinical trials among racial/ethnic minorities and smokers with lower income. METHODS: Participants were enrolled in a randomized trial of the efficacy of an Internet smoking cessation program between June 2005 and September 2006. Following a baseline telephone assessment and randomization into the parent trial, participants were recruited to the reliability substudy. In phase I of recruitment, all participants in the parent trial were recruited to the substudy; in phase II, all consecutive racial/ethnic minority participants in the parent trial were recruited. Race and ethnicity were assessed via self-report using two standard items from the US Office of Management and Budget. An email was sent 2 days after the telephone assessment with a link to the Internet survey. Measures examined were quit methods, perceived stress, depression, social support, smoking temptations, alcohol use, perceived health status, and income. Internal consistency and test-retest reliability of Internet- versus telephone-administered measures were examined within four strata defined by race/ethnicity (non-Hispanic White, racial/ethnic minority) and annual household income (US $40,000 or less, more than $40,000). RESULTS: Of the 442 individuals invited, 319 participated (72% response rate): 52.4% were non-Hispanic White, 22.9% Black, 11.6% Hispanic, 7.8% Asian, 4.4% American Indian / Alaska Native, and 1% Native Hawaiian / Other Pacific Islander. About half (49.4%) reported an annual household income of US $40,000 or less, and 25.7% had a high school degree or less. Test-retest reliability was satisfactory to excellent across all strata for the majority of measures examined: 9 of 12 continuous variables had intraclass correlation coefficients > or = 0.70, and 10 of 18 binary variables and both ordinal variables had kappa coefficients > or = 0.70. Test-retest reliability of several quit methods varied across strata. CONCLUSIONS: Race/ethnicity and income do not affect the psychometric properties of most Internet-administered measures examined. This knowledge adds to the confidence of conducting Web-based smoking cessation research and strengthens the scientific rigor of collecting information via the Internet on racial/ethnic minority and low-income subgroups. TRIAL REGISTRATION: clinicaltrials.gov NCT00282009 (parent trial).
Descriptors
Adult, Confidence Intervals, Female, Health Behavior/ethnology, Humans, Internet/utilization, Male, Middle Aged, Patient Education as Topic/methods, Patient Participation/statistics & numerical data, Poverty, Psychometrics, Referral and Consultation/statistics & numerical data, Reproducibility of Results, Smoking/ethnology, Smoking Cessation/ethnology/methods, Surveys and Questionnaires, Telephone/utilization, Tobacco Use Disorder/ethnology/rehabilitation, Treatment Outcome, United States/epidemiology
Links
Book Title
Database
Publisher
Data Source
Authors
Graham,A. L., Papandonatos,G. D.
Original/Translated Title
URL
Date of Electronic
20080326
PMCID
PMC2483847
Editors
A web-based sexual violence bystander intervention for male college students: randomized controlled trial 2014 Georgia State University, School of Public Health, Atlanta, GA, United States. lsalazar1@gsu.edu.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of medical Internet research
Periodical, Abbrev.
J.Med.Internet Res.
Pub Date Free Form
5-Sep
Volume
16
Issue
9
Start Page
e203
Other Pages
Notes
LR: 20150928; ClinicalTrials.gov/NCT01903876; GR: R49 CE000892/CE/NCIPC CDC HHS/United States; JID: 100959882; OID: NLM: PMC4180355; OTO: NOTNLM; 2014/03/25 [received]; 2014/08/16 [accepted]; 2014/07/12 [revised]; epublish
Place of Publication
Canada
ISSN/ISBN
1438-8871; 1438-8871
Accession Number
PMID: 25198417
Language
eng
SubFile
Journal Article; Randomized Controlled Trial; Research Support, U.S. Gov't, P.H.S.; IM
DOI
10.2196/jmir.3426 [doi]
Output Language
Unknown(0)
PMID
25198417
Abstract
BACKGROUND: Bystander intervention approaches offer promise for reducing rates of sexual violence on college campuses. Most interventions are in-person small-group formats, which limit their reach and reduce their overall public health impact. OBJECTIVE: This study evaluated the efficacy of RealConsent, a Web-based bystander approach to sexual violence prevention, in enhancing prosocial intervening behaviors and preventing sexual violence perpetration. METHODS: A random probability sample of 743 male undergraduate students (aged 18 to 24 years) attending a large, urban university located in the southeastern United States was recruited online and randomized to either RealConsent (n=376) or a Web-based general health promotion program (n=367). Participants were surveyed online at baseline, postintervention, and 6-months postintervention. RealConsent was delivered via a password-protected Web portal that contained six 30-minute media-based and interactive modules covering knowledge of informed consent, communication skills regarding sex, the role of alcohol and male socialization in sexual violence, empathy for rape victims, and bystander education. Primary outcomes were self-reported prosocial intervening behaviors and sexual violence perpetration. Secondary outcomes were theoretical mediators (eg, knowledge, attitudes). RESULTS: At 6-month follow-up RealConsent participants intervened more often (P=.04) and engaged in less sexual violence perpetration (P=.04) compared to controls. In addition, RealConsent participants reported greater legal knowledge of sexual assault (P<.001 greater="" knowledge="" of="" effective="" consent="" less="" rape="" myths="" empathy="" for="" victims="" negative="" date="" attitudes="" hostility="" toward="" women="" intentions="" to="" intervene="" hyper-gender="" ideology="" positive="" outcome="" expectancies="" nonconsensual="" sex="" more="" intervening="" and="" comfort="" with="" other="" men="" inappropriate="" behaviors="" conclusions:="" our="" results="" support="" the="" efficacy="" realconsent.="" due="" its="" web-based="" format="" realconsent="" has="" potential="" broad-based="" dissemination="" thereby="" increasing="" overall="" public="" health="" impact="" on="" sexual="" violence.="" trial="" registration:="" clinicaltrials.gov:="" nct01903876="" http:="" by="" webcite="" at="">
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Salazar,L.F., Vivolo-Kantor,A., Hardin,J., Berkowitz,A.
Original/Translated Title
URL
Date of Electronic
20140905
PMCID
PMC4180355
Editors
E-Cigarette Use Among Never-Smoking California Students 2015 Georgiana Bostean is with the Sociology Department and Environmental Science & Policy Program, Chapman University, Orange, CA. Dennis R. Trinidad is with the Department of Family Medicine and Public Health, University of California, San Diego. William J.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
American Journal of Public Health
Periodical, Abbrev.
Am.J.Public Health
Pub Date Free Form
Dec
Volume
105
Issue
12
Start Page
2423
Other Pages
2425
Notes
LR: 20160809; GR: 1P50HL105188#6094/HL/NHLBI NIH HHS/United States; JID: 1254074; CIN: Am J Public Health. 2016 May;106(5):e13-4. PMID: 27049426; CIN: Am J Public Health. 2016 May;106(5):e13. PMID: 27049425; 2015/10/15 [aheadofprint]; ppublish
Place of Publication
United States
ISSN/ISBN
1541-0048; 0090-0036
Accession Number
PMID: 26469671
Language
eng
SubFile
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; AIM; IM
DOI
10.2105/AJPH.2015.302899 [doi]
Output Language
Unknown(0)
PMID
26469671
Abstract
We determined the extent to which adolescents who have never used tobacco try e-cigarettes. Data on the prevalence and correlates of e-cigarette use among 482,179 California middle and high school students are from the 2013-2014 California Healthy Kids Survey. Overall, 24.4% had ever used e-cigarettes (13.4% have never used tobacco and 11.0% have used tobacco), and 12.9% were current e-cigarette users (5.9% have never used tobacco). Among those who have never used tobacco, males and older students were more likely to use e-cigarettes than females and younger students. Hispanics (odds ratio [OR] = 1.60; confidence interval [CI] = 1.53, 1.67) and those of other races (OR = 1.24; CI = 1.19, 1.29) were more likely than Whites to have ever used e-cigarettes, but only among those who had never used smokeless tobacco and never smoked a whole cigarette. E-cigarette use is very prevalent among California students who have never smoked tobacco, especially among Hispanic and other race students, males, and older students.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Bostean,G., Trinidad,D.R., McCarthy,W.J.
Original/Translated Title
URL
Date of Electronic
20151015
PMCID
Editors
Gold nanoparticles production using reactor and cyclotron based methods in assessment of (196,198)Au production yields by (197)Au neutron absorption for therapeutic purposes 2016 Gerash Research Institute-Mother Home of Gerash, Cellular and Molecular Gerash Research Center, Gerash School of Medical Science, Shiraz University of Medical Sciences, P.O. BOX: 7441955666, Shiraz, Iran. Electronic address: abkhorshidi@yahoo.com.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Materials science & engineering.C, Materials for biological applications
Periodical, Abbrev.
Mater.Sci.Eng.C.Mater.Biol.Appl.
Pub Date Free Form
1-Nov
Volume
68
Issue
Start Page
449
Other Pages
454
Notes
CI: Copyright (c) 2016; JID: 101484109; OTO: NOTNLM; 2016/05/15 [received]; 2016/06/05 [accepted]; 2016/06/06 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
1873-0191; 0928-4931
Accession Number
PMID: 27524041
Language
eng
SubFile
Journal Article; IM
DOI
10.1016/j.msec.2016.06.018 [doi]
Output Language
Unknown(0)
PMID
27524041
Abstract
Medical nano-gold radioisotopes is produced regularly using high-flux nuclear reactors, and an accelerator-driven neutron activator can turn out higher yield of (197)Au(n,gamma)(196,198)Au reactions. Here, nano-gold production via radiative/neutron capture was investigated using irradiated Tehran Research Reactor flux and also simulated proton beam of Karaj cyclotron in Iran. (197)Au nano-solution, including 20nm shaped spherical gold and water, was irradiated under Tehran reactor flux at 2.5E+13n/cm(2)/s for (196,198)Au activity and production yield estimations. Meanwhile, the yield was examined using 30MeV proton beam of Karaj cyclotron via simulated new neutron activator containing beryllium target, bismuth moderator around the target, and also PbF2 reflector enclosed the moderator region. Transmutation in (197)Au nano-solution samples were explored at 15 and 25cm distances from the target. The neutron flux behavior inside the water and bismuth moderators was investigated for nano-gold particles transmutation. The transport of fast neutrons inside bismuth material as heavy nuclei with a lesser lethargy can be contributed in enhanced nano-gold transmutation with long duration time than the water moderator in reactor-based method. Cyclotron-driven production of betaeta-emitting radioisotopes for brachytherapy applications can complete the nano-gold production technology as a safer approach as compared to the reactor-based method.
Descriptors
Links
Book Title
Database
Publisher
Elsevier B.V
Data Source
Authors
Khorshidi,A.
Original/Translated Title
URL
Date of Electronic
20160606
PMCID
Editors
Impact of national smoke-free legislation on home smoking bans: findings from the International Tobacco Control Policy Evaluation Project Europe Surveys 2013 German Cancer Research Center, Unit Cancer Prevention and WHO Collaborating Centre for Tobacco Control, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany. u.mons@dkfz.de
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Tobacco control
Periodical, Abbrev.
Tob.Control
Pub Date Free Form
May
Volume
22
Issue
e1
Start Page
e2
Other Pages
9
Notes
LR: 20150225; GR: 57897/Canadian Institutes of Health Research/Canada; GR: 79551/Canadian Institutes of Health Research/Canada; GR: C312/A6465/Cancer Research UK/United Kingdom; GR: P01 CA138389/CA/NCI NIH HHS/United States; GR: P01 CA138389/CA/NCI NIH HH
Place of Publication
England
ISSN/ISBN
1468-3318; 0964-4563
Accession Number
PMID: 22331456
Language
eng
SubFile
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; IM
DOI
10.1136/tobaccocontrol-2011-050131 [doi]
Output Language
Unknown(0)
PMID
22331456
Abstract
OBJECTIVES: To measure changes in prevalence and predictors of home smoking bans (HSBs) among smokers in four European countries after the implementation of national smoke-free legislation. DESIGN: Two waves of the International Tobacco Control Policy Evaluation Project Europe Surveys, which is a prospective panel study. Pre- and post-legislation data were used from Ireland, France, Germany and the Netherlands. Two pre-legislation waves from the UK were used as control. PARTICIPANTS: 4634 respondents from the intervention countries and 1080 from the control country completed both baseline and follow-up and were included in the present analyses. METHODS: Multiple logistic regression models to identify predictors of having or of adopting a total HSB, and Generalised Estimating Equation models to compare patterns of change after implementation of smoke-free legislation to a control country without such legislation. RESULTS: Most smokers had at least partial smoking restrictions in their home, but the proportions varied significantly between countries. After implementation of national smoke-free legislation, the proportion of smokers with a total HSB increased significantly in all four countries. Among continuing smokers, the number of cigarettes smoked per day either remained stable or decreased significantly. Multiple logistic regression models indicated that having a young child in the household and supporting smoking bans in bars were important correlates of having a pre-legislation HSB. Prospective predictors of imposing a HSB between survey waves were planning to quit smoking, supporting a total smoking ban in bars and the birth of a child. Generalised Estimating Equation models indicated that the change in total HSB in the intervention countries was greater than that in the control country. CONCLUSIONS: The findings suggest that smoke-free legislation does not lead to more smoking in smokers' homes. On the contrary, our findings demonstrate that smoke-free legislation may stimulate smokers to establish total smoking bans in their homes.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Mons,U., Nagelhout,G.E., Allwright,S., Guignard,R., van den Putte,B., Willemsen,M.C., Fong,G.T., Brenner,H., Potschke-Langer,M., Breitling,L.P.
Original/Translated Title
URL
Date of Electronic
20120213
PMCID
PMC4010876
Editors
Mainstream smoke of the waterpipe: does this environmental matrix reveal as significant source of toxic compounds? 2011 German Federal Institute for Risk Assessment (BfR), Department of Product Safety, Thielallee 88-92, 14195 Berlin, Germany. Jens.Schubert@bfr.bund.de
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Toxicology letters
Periodical, Abbrev.
Toxicol.Lett.
Pub Date Free Form
10-Sep
Volume
205
Issue
3
Start Page
279
Other Pages
284
Notes
LR: 20151119; CI: Copyright (c) 2011; JID: 7709027; 0 (4-(methylnitrosamino)-1-(3-pyridyl)-1-butan-1-ol); 0 (Carcinogens); 0 (Hygroscopic Agents); 0 (Nitrosamines); 0 (Polycyclic Hydrocarbons, Aromatic); 0 (Pyridines); 0 (Smoke); 059QF0KO0R (Water); 3417W
Place of Publication
Netherlands
ISSN/ISBN
1879-3169; 0378-4274
Accession Number
PMID: 21712083
Language
eng
SubFile
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.toxlet.2011.06.017 [doi]
Output Language
Unknown(0)
PMID
21712083
Abstract
In recent years the number of waterpipe smokers has increased substantially worldwide. Here we report on the concentrations of tobacco-specific nitrosamines (TSNAs) and polycyclic aromatic hydrocarbons (PAHs) in waterpipe smoke and the analysis of selected biomarkers indicative for the body burden in waterpipe users. We further identify high amounts of unburned humectants (glycerol and propylene glycol) in the waterpipe smoke as main part of the so-called "tar" fraction. These results give cause for serious concern. For standardization we applied a machine smoking protocol. Smoke was collected on glass fiber filters and analyzed for nicotine, water, humectants, TSNAs, and PAHs. In addition, we determined carbon monoxide and found high amounts in the smoke being causative for high levels of carboxyhemoglobin (COHb) in the blood of smokers. In comparison to the reference cigarette 3R4F, the nicotine contents were 10-times higher, but TSNA levels were found lower in waterpipe smoke. This finding explained the low levels of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol detected in the urine of waterpipe smokers. Finally, the levels of benzo[a]pyrene were three times higher in waterpipe smoke compared to the reference cigarette. Altogether, the data presented in this study point to the health hazards associated with the consumption of waterpipes.
Descriptors
Links
Book Title
Database
Publisher
Elsevier Ireland Ltd
Data Source
Authors
Schubert,J., Hahn,J., Dettbarn,G., Seidel,A., Luch,A., Schulz,T.G.
Original/Translated Title
URL
Date of Electronic
20110625
PMCID
Editors
Prevalence of patent foramen ovale (PFO) and MRI-lesions in mild neurological decompression sickness (type B-DCS/AGE) 2008 German Naval Medical Institute, Kiel-Kronshagen, Germany.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc
Periodical, Abbrev.
Undersea Hyperb.Med.
Pub Date Free Form
May-Jun
Volume
35
Issue
3
Start Page
197
Other Pages
205
Notes
JID: 9312954; ppublish
Place of Publication
United States
ISSN/ISBN
1066-2936; 1066-2936
Accession Number
PMID: 18619115
Language
eng
SubFile
Journal Article; IM; S
DOI
Output Language
Unknown(0)
PMID
18619115
Abstract
BACKGROUND: Neurological decompression sickness (DCS/AGE) may cover two variants with either severer and probably central nervous (Type A) or milder and sometimes doubtful neurological symptoms (Type B). The pathophysiology of the Type B-DCS/AGE might be different from the Type A-variant. In Type A-DCS/AGE a higher PFO-prevalence (patent foramen ovale) points towards an embolic origin of the Type A-symptomatology. This is not necessarily expected for the Type B-DCS/AGE if the pathophysiology here is micro-embolic or even non-embolic. METHODS: 18 patients with Type B-DCS/AGE were tested against matched controls for presence and size of a PFO with echocardiography and transcranial ultrasound with echo-contrast. Prevalence and number of Type A-brain lesions were visualized by cranial MRI as possible sequelae from gas-embolic events. RESULTS: PFO-prevalence in both groups, the patients with Type B-DCS/AGE (5/18) as well as the controls (7/18) was similar to published PFO-prevalences in normals without any difference between patients and controls (p = 0.725). Also the number of MRI-lesions (ACFs) was the same for Type B-DCS/AGE cases (15 ACFs in 5 patients) and controls (37 ACFs in 8 divers). CONCLUSION: Indirect findings suggesting embolic brain injuries are found with similar frequency in patients with Type B-DCS/AGE and normal controls, which is in contrast to data about Type A-DCS/AGE. This is compatible with different pathophysiological mechanisms involved in the Type A- and Type B-DCS/AGE.
Descriptors
Adult, Brain Diseases/diagnosis/physiopathology, Case-Control Studies, Decompression Sickness/physiopathology, Echocardiography, Embolism, Air/diagnosis/physiopathology, Female, Foramen Ovale, Patent/diagnosis/epidemiology/physiopathology, Humans, Intracranial Embolism/diagnosis/physiopathology, Magnetic Resonance Imaging, Male, Prevalence, Statistics, Nonparametric
Links
Book Title
Database
Publisher
Data Source
Authors
Koch,A. E., Kirsch,H., Reuter,M., Warninghoff,V., Rieckert,H., Deuschl,G.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Antifungal activity of amphotericin B and voriconazole against the biofilms and biofilm-dispersed cells of Candida albicans employing a newly developed in vitro pharmacokinetic model 2015 German University in Cairo, GUC, Faculty of Pharmacy and Biotechnology, Department of Microbiology, Immunology and Biotechnology, Al-Tagmoa Al-Khamis, New Cairo City, Egypt. mohamed.el-azizi@guc.edu.eg.; German University in Cairo, GUC, Faculty of Pharmac
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Annals of clinical microbiology and antimicrobials
Periodical, Abbrev.
Ann.Clin.Microbiol.Antimicrob.
Pub Date Free Form
3-Apr
Volume
14
Issue
Start Page
21
Other Pages
015-0083-3
Notes
LR: 20150420; JID: 101152152; 0 (Antifungal Agents); 7XU7A7DROE (Amphotericin B); JFU09I87TR (Voriconazole); OID: NLM: PMC4389768; 2014/11/07 [received]; 2015/03/23 [accepted]; 2015/04/03 [aheadofprint]; epublish
Place of Publication
England
ISSN/ISBN
1476-0711; 1476-0711
Accession Number
PMID: 25885806
Language
eng
SubFile
Journal Article; IM
DOI
10.1186/s12941-015-0083-3 [doi]
Output Language
Unknown(0)
PMID
25885806
Abstract
BACKGROUND: Candida albicans is a common cause of a variety of superficial and invasive disseminated infections the majority of which are associated with biofilm growth on implanted devices. The aim of the study is to evaluate the activity of amphotericin B and voriconazole against the biofilm and the biofilm-dispersed cells of Candida albicans using a newly developed in vitro pharmacokinetic model which simulates the clinical situation when the antifungal agents are administered intermittently. METHODS: RPMI medium containing 1-5 X 10(6) CFU/ml of C. albicans was continuously delivered to the device at 30 ml/h for 2 hours. The planktonic cells were removed and biofilms on the catheter were kept under continuous flow of RPMI medium at 10 ml/h. Five doses of amphotericin B or voriconazole were delivered to 2, 5 and 10 day-old biofilms at initial concentrations (2 and 3 mug/ml respectively) that were exponentially diluted. Dispersed cells in effluents from the device were counted and the adherent cells on the catheter were evaluated after 48 h of the last dose. RESULTS: The minimum inhibitory concentration of voriconazole and amphotericin B against the tested isolate was 0.0325 and 0.25 mug/ml respectively. Amphotericin B significantly reduced the dispersion of C. albicans cells from the biofilm. The log10 reduction in the dispersed cells was 2.54-3.54, 2.30-3.55, and 1.94-2.50 following addition of 5 doses of amphotericin B to 2-, 5- and 10-day old biofilms respectively. The number of the viable cells within the biofilm was reduced by 18 (+/-7.63), 5 and 4% following addition of the 5 doses of amphotericin B to the biofilms respectively. Voriconazole showed no significant effect on the viability of C. albicans within the biofilm. CONCLUSION: Both antifungal agents failed to eradicate C. albicans biofilm or stop cell dispersion from them and the resistance progressed with maturation of the biofilm. These findings go along with the need for removal of devices in spite of antifungal therapy in patients with device-related infection. This is the first study which investigates the effects of antifungal agents on the biofilm and biofilm-dispersion of C. albicans in an in vitro pharmacokinetic biofilm model.
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El-Azizi,M., Farag,N., Khardori,N.
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20150403
PMCID
PMC4389768
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