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Repurposing as a means to increase the activity of amphotericin B and caspofungin against Candida albicans biofilms 2014 Centre of Microbial and Plant Genetics, CMPG, KU Leuven, Kasteelpark Arenberg 20, Box 2460, 3001 Heverlee, Belgium.
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Journal Article
Periodical, Full
The Journal of antimicrobial chemotherapy
Periodical, Abbrev.
J.Antimicrob.Chemother.
Pub Date Free Form
Apr
Volume
69
Issue
4
Start Page
1035
Other Pages
1044
Notes
JID: 7513617; 0 (Androstenes); 0 (Antifungal Agents); 0 (Echinocandins); 7NFE54O27T (Toremifene); 7XU7A7DROE (Amphotericin B); F0XDI6ZL63 (caspofungin); K7V8Y90G0H (perhexiline maleate); KU65374X44 (Perhexiline); N295J34A25 (drospirenone); OTO: NOTNLM; 20
Place of Publication
England
ISSN/ISBN
1460-2091; 0305-7453
Accession Number
PMID: 24284780
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1093/jac/dkt449 [doi]
Output Language
Unknown(0)
PMID
24284780
Abstract
OBJECTIVES: Biofilms of Candida species, often formed on medical devices, are generally resistant to currently available antifungal drugs. The aim of this study was to identify compounds that increase the activity of amphotericin B and caspofungin, commonly used antifungal agents, against Candida biofilms. METHODS: A library containing off-patent drugs was screened for compounds, termed enhancers, that increase the in vitro activity of amphotericin B against Candida albicans biofilms. Biofilms were grown in 96-well plates and growth was determined by the cell titre blue assay. Synergy between identified enhancers and antifungal agents was further characterized in vitro using fractional inhibitory concentration index (FICI) values and in vivo using a worm biofilm infection model. In light of the application of these enhancers onto implants, their possible effect on the growth potential of MG63 osteoblast-like cells was assessed. RESULTS: Pre-incubation of C. albicans biofilms with subinhibitory concentrations of the enhancers drospirenone, perhexiline maleate or toremifene citrate significantly increased the activity of amphotericin B or caspofungin (FICI
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Authors
Delattin,N., De Brucker,K., Vandamme,K., Meert,E., Marchand,A., Chaltin,P., Cammue,B.P., Thevissen,K.
Original/Translated Title
URL
Date of Electronic
20131127
PMCID
Editors
Effect of shisha vs. cigarette smoking on endothelial function by brachial artery duplex ultrasonography: an observational study 2013 Department of Cardiology and Radiodiagnosis, Faculty of Medicine, Ain Shams University, Cairo-Egypt. Ghadaselim23@yahoo.com.
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Journal Article
Periodical, Full
Anadolu kardiyoloji dergisi : AKD = the Anatolian journal of cardiology
Periodical, Abbrev.
Anadolu Kardiyol Derg.
Pub Date Free Form
Dec
Volume
13
Issue
8
Start Page
759
Other Pages
765
Notes
JID: 101095069; CIN: Anadolu Kardiyol Derg. 2014 Jun;14(4):408. PMID: 24818978; 2013/11/25 [aheadofprint]; ppublish
Place of Publication
Turkey
ISSN/ISBN
1308-0032; 1302-8723
Accession Number
PMID: 24287354
Language
eng
SubFile
Journal Article; Observational Study; IM
DOI
10.5152/akd.2013.4499 [doi]
Output Language
Unknown(0)
PMID
24287354
Abstract
OBJECTIVE: In this study, we sought to investigate the effect of shisha smoking on endothelial function compared to cigarettes, using brachial artery ultrasound (BAUS) imaging in asymptomatic young adults with no other cardiovascular risk factors. METHODS: This is an observational case-control study where 30 young shisha smokers, 30 cigarette smokers and 10 healthy, non-smokers, age- matched subjects between 25-35 years old with no cardiovascular risk factors were recruited from all around Egypt. Flow-mediated dilation was assessed using brachial artery duplex ultrasonography. RESULTS: FMD% was significantly impaired among shisha smokers compared to cigarette smokers and non-smokers cigarettes (7.9 +/- 3.8% vs. 12 +/- 3.4% and 21.5 +/- 2.5% respectively p
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Database
Publisher
Data Source
Authors
Selim,G.M., Elia,R.Z., El Bohey,A.S., El Meniawy,K.A.
Original/Translated Title
URL
Date of Electronic
20131125
PMCID
Editors
Tobacco harm reduction: the need for new products that can compete with cigarettes 2014 Fagerstrom Consulting AB, Vaxholm, Sweden. Electronic address: karl.fagerstrom@swipnet.se.; Nicoventures Limited, London, UK. Electronic address: kevin.bridgman@nicoventures.co.uk.
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Print(0)
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Journal Article
Periodical, Full
Addictive Behaviors
Periodical, Abbrev.
Addict.Behav.
Pub Date Free Form
Mar
Volume
39
Issue
3
Start Page
507
Other Pages
511
Notes
LR: 20151119; CI: Copyright (c) 2013; JID: 7603486; 0 (Nicotinic Agonists); 6M3C89ZY6R (Nicotine); OTO: NOTNLM; 2013/07/29 [received]; 2013/10/28 [revised]; 2013/11/01 [accepted]; 2013/11/10 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
1873-6327; 0306-4603
Accession Number
PMID: 24290207
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; Review; IM
DOI
10.1016/j.addbeh.2013.11.002 [doi]
Output Language
Unknown(0)
PMID
24290207
Abstract
Over the last 50 years, the concept of tobacco harm reduction has been well established. It is now understood that nicotine itself is not very harmful and nicotine replacement therapy products have been widely used as an aid to quit, reduce to quit or temporarily abstain from smoking for many years. The popularity of the unlicensed electronic cigarette has increased despite an unknown risk profile and sinus use in Sweden provides strong evidence in support of a harm reduction strategy. The regulatory environment around harm reduction has changed in the UK and is continuing to evolve across the globe. The need for more appealing, licensed nicotine products capable of competing with cigarettes sensorially, pharmacologically and behaviourally is considered by many to be the way forward. The significant positive impact on public health that could be gained from encouraging people to switch from cigarettes to licensed medicinal nicotine products cannot be ignored.
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Book Title
Database
Publisher
Elsevier Ltd
Data Source
Authors
Fagerstrom,K.O., Bridgman,K.
Original/Translated Title
URL
Date of Electronic
20131110
PMCID
Editors
Comparison of plasmids coharboring 16s rrna methylase and extended-spectrum beta-lactamase genes among Escherichia coli isolates from pets and poultry 2013 College of Veterinary Medicine, National Reference Laboratory of Veterinary Drug Residues (SCAU), South China Agricultural University, Guangzhou 510642, People's Republic of China.
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Print(0)
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Journal Article
Periodical, Full
Journal of food protection
Periodical, Abbrev.
J.Food Prot.
Pub Date Free Form
Dec
Volume
76
Issue
12
Start Page
2018
Other Pages
2023
Notes
LR: 20151119; JID: 7703944; 0 (Escherichia coli Proteins); 0 (RNA, Ribosomal, 16S); EC 2.1.1.- (Methyltransferases); EC 2.1.1.- (RmtB protein, E coli); EC 2.1.1.230 (rRNA (adenosine-O-2'-)methyltransferase); EC 3.5.2.6 (beta-Lactamases); ppublish
Place of Publication
United States
ISSN/ISBN
1944-9097; 0362-028X
Accession Number
PMID: 24290675
Language
eng
SubFile
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.4315/0362-028X.JFP-13-200 [doi]
Output Language
Unknown(0)
PMID
24290675
Abstract
A total of 247 Escherichia coli isolates (148 from diseased or dead poultry and 99 from diseased pets in the People's Republic of China) were screened for extended-spectrum beta-lactamase (ESBL) determinants by PCR and sequencing. Then, 16S rRNA methylase genes were detected among ESBL-producing isolates. Clonal relatedness of the E. coli isolates was examined by pulsed-field gel electrophoresis. Conjugation experiments were performed to investigate the association of 16S rRNA methylases and ESBLs, and plasmid contents were also characterized. Among 247 E. coli isolates, 74 (29.96%) isolates were positive for blaCTX-M genes, 42 from pets (12 from cats and 30 from dogs) and 32 from poultry (12 from chickens and 20 from ducks). The most common CTX-M type in isolates from pets was blaCTX-M-14, whereas blaCTX-M-27 was the most common for poultry. rmtB was dectected in 39 of the 74 blaCTX-M-positive isolates, 18 from pets and 21 from poultry. One strain from a pet was found to harbor blaCTX-M-14, blaCTX-M-15, and rmtB. blaCTX-M and rmtB were found to be colocated on the same transferable plasmid in 16 isolates. These genes were on the same or similar plasmids (eight F2:A-:B- and two IncN) in isolates from ducks, whereas they were colocated on the similar F2:A-:B- or similar F33:A-:B- plasmids in isolates of pets origin. In conclusion, similar F2:A-:B- plasmids and similar F33:A-:B- plasmids are responsible for the dissemination of both rmtB and blaCTX-Mgenes in E. coli isolates from poultry and pets, respectively.
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Data Source
Authors
Liao,X.P., Liu,B.T., Yang,Q.E., Sun,J., Li,L., Fang,L.X., Liu,Y.H.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Diversity of lactic acid bacteria associated with fresh coffee cherries in Taiwan 2014 Department of Biotechnology, Ming Chuan University, No. 5 De-Ming Road, Gui-Shan Township, Taoyuan, 333, Taiwan.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Current microbiology
Periodical, Abbrev.
Curr.Microbiol.
Pub Date Free Form
Apr
Volume
68
Issue
4
Start Page
440
Other Pages
447
Notes
JID: 7808448; 0 (Coffee); 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 2013/08/06 [received]; 2013/10/13 [accepted]; 2013/12/01 [aheadofprint]; ppublish
Place of Publication
United States
ISSN/ISBN
1432-0991; 0343-8651
Accession Number
PMID: 24292770
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1007/s00284-013-0495-2 [doi]
Output Language
Unknown(0)
PMID
24292770
Abstract
A total of 102 lactic acid bacteria (LAB) were isolated from three different coffee farms in Taiwan. These isolates were classified and identified by the restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Heterofermentative Leuconostoc, and Weissella species were the most common LAB found in two farms located at an approximate altitude of 800 m. Lactococcus lactis subsp. lactis was the most common LAB found in the remaining farm was located at an approximate altitude of 1,200 m. It is therefore suggested that the altitude and climate may affect the distribution of LAB. On the basis of phylogenetic analysis, two strains included in the genera Enterococcus were considered as two potential novel species or subspecies. In addition, a total of 34 isolates showed the antifungal activity against Aspergillus flavus. Moreover, seven Lactococcus lactis subsp. lactis strains and one Enterococcus faecalis strain were found to have bacteriocin-like inhibitory substance-producing capability. These results suggest that various LAB are associated with fresh coffee cherries in Taiwan. Some of the isolates found in this study showed potential as antifungal agents.
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Data Source
Authors
Leong,K.H., Chen,Y.S., Pan,S.F., Chen,J.J., Wu,H.C., Chang,Y.C., Yanagida,F.
Original/Translated Title
URL
Date of Electronic
20131201
PMCID
Editors
Comparison of tobacco-containing and tobacco-free waterpipe products: effects on human alveolar cells 2014 Department of Mechanical Engineering, American University of Beirut, Beirut, Lebanon;
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco
Periodical, Abbrev.
Nicotine Tob.Res.
Pub Date Free Form
Apr
Volume
16
Issue
4
Start Page
496
Other Pages
499
Notes
LR: 20150422; GR: R01 DA025659/DA/NIDA NIH HHS/United States; GR: R01CA120142/CA/NCI NIH HHS/United States; GR: R01DA025659/DA/NIDA NIH HHS/United States; JID: 9815751; 0 (Smoke); OID: NLM: PMC4007115; 2013/12/03 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
1469-994X; 1462-2203
Accession Number
PMID: 24302635
Language
eng
SubFile
Comparative Study; Journal Article; Research Support, N.I.H., Extramural; IM
DOI
10.1093/ntr/ntt193 [doi]
Output Language
Unknown(0)
PMID
24302635
Abstract
INTRODUCTION: In recent years, a class of products marketed as "tobacco-free" alternatives for the "health conscious user" has become widely available for waterpipe (hookah, narghile, or shisha) smoking. Their adoption may be in part driven by regulations banning tobacco smoking in public places and by an increasing awareness of the hazards of waterpipe tobacco smoking. Although these products are presented in advertising as a "healthier" choice, very little is known about their health effects. METHODS: In this study, we compared the effects of smoke generated with tobacco-free and conventional tobacco-derived products on human alveolar cells. Smoke was generated with a smoking machine that precisely mimicked the puffing behavior of 15 experienced waterpipe smokers when they used conventional waterpipe tobacco products of their choice and flavor-matched tobacco-free products. Human alveolar epithelial cells (A549) were treated with particulate matter sampled from the smoke, and the effects on cell cycle, proliferation, and doubling time were measured during the subsequent 72hr. RESULTS: We found that smoke from both types of waterpipe products markedly reduced cell proliferation, caused cell cycle arrest at G0/G1, and increased cell doubling time. There were no significant differences across product in any measure. CONCLUSION: Tobacco-free and tobacco-based waterpipe products exert substantial and similar deleterious effects on human lung cells. This study adds to the nascent evidence base indicating that except for exposure to nicotine and its derivatives, use of tobacco-free waterpipe products does not present a reduced health risk relative to the use of conventional tobacco-based products.
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Database
Publisher
Data Source
Authors
Shihadeh,A., Eissenberg,T., Rammah,M., Salman,R., Jaroudi,E., El-Sabban,M.
Original/Translated Title
URL
Date of Electronic
20131203
PMCID
PMC4007115
Editors
Predominance of IncL/M and IncF plasmid types among CTX-M-ESBL-producing Escherichia coli and Klebsiella pneumoniae in Bulgarian hospitals 2014 Department of Medical Microbiology, Medical University, Sofia, Bulgaria.
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Print(0)
Ref Type
Journal Article
Periodical, Full
APMIS : Acta Pathologica, Microbiologica, et Immunologica Scandinavica
Periodical, Abbrev.
APMIS
Pub Date Free Form
Jul
Volume
122
Issue
7
Start Page
608
Other Pages
615
Notes
CI: (c) 2013; JID: 8803400; EC 3.5.2.- (beta-lactamase CTX-M-15); EC 3.5.2.- (beta-lactamase CTX-M-3); EC 3.5.2.- (beta-lactamase SHV-12); EC 3.5.2.- (beta-lactamase TEM-3); EC 3.5.2.6 (beta-Lactamases); OTO: NOTNLM; 2013/05/15 [received]; 2013/08/30 [acc
Place of Publication
Denmark
ISSN/ISBN
1600-0463; 0903-4641
Accession Number
PMID: 24303846
Language
eng
SubFile
Journal Article; IM
DOI
10.1111/apm.12204 [doi]
Output Language
Unknown(0)
PMID
24303846
Abstract
Our objective was to investigate the plasmid replicon-types involved in spread of ESBLs among Bulgarian Klebsiella pneumoniae and Escherichia coli. Sixty-three isolates, with transferable beta-lactam resistance determinants, collected between 2007 and 2009 in six medical institutions, were analysed with respect to their antimicrobial susceptibility, ESBL-, RAPD-, and plasmid replicon-type. Phylogenetic typing and screening for the O25b-ST131 lineage were carried out for E. coli. The predominant ESBLs were CTX-M-15 (81%) among E. coli and CTX-M-3 (58%) among K. pneumoniae. Other sporadically found ESBLs were SHV-12 and TEM-139, and for the first time in Bulgaria, CTX-M-1 and CTX-M-14. Replicon typing revealed that plasmids carrying blaCTX-M-3 exclusively belonged to IncL/M-type, while blaCTX-M-15 was predominantly (94%) associated with IncF-type plasmids. Among E. coli, 59% of the isolates were clonally related. Isolates of that cluster produced CTX-M-15, belonged to the O25b-ST131 lineage, predominantly harboured plasmids with the FIA replicon, and were found in five centres. Among CTX-M-3-producing K. pneumoniae, two prevailing RAPD-types were found, one remained restricted to one centre and the second was found in three centres. The incompatibility groups IncN and IncA/C linked with blaSHV-12 respectively blaTEM-139 were found only once. To the best of our knowledge, this is the first detailed investigation of plasmids carrying ESBL genes among Bulgarian isolates demonstrating wide distribution of conjugative IncF plasmids among CTX-M-15-producing E. coli and IncL/M plasmids among CTX-M-3 positive K. pneumoniae isolates.
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Book Title
Database
Publisher
APMIS. Published by John Wiley & Sons Ltd
Data Source
Authors
Markovska,R., Schneider,I., Ivanova,D., Mitov,I., Bauernfeind,A.
Original/Translated Title
URL
Date of Electronic
20131205
PMCID
Editors
Prevalence of and factors influencing smoking among medical and non-medical students in Tbilisi, Georgia 2013 Tbilisi State Medical University, Tbilisi, Georgia.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Jornal brasileiro de pneumologia : publicacao oficial da Sociedade Brasileira de Pneumologia e Tisilogia
Periodical, Abbrev.
J.Bras.Pneumol.
Pub Date Free Form
Sep-Oct
Volume
39
Issue
5
Start Page
579
Other Pages
584
Notes
LR: 20151119; JID: 101222274; OID: NLM: PMC4075884; 2012/10/11 [received]; 2013/08/19 [accepted]; ppublish
Place of Publication
Brazil
ISSN/ISBN
1806-3756; 1806-3713
Accession Number
PMID: 24310631
Language
eng; por
SubFile
Journal Article; IM
DOI
10.1590/S1806-37132013000500008 [doi]
Output Language
Unknown(0)
PMID
24310631
Abstract
OBJECTIVE: Smoking is a serious problem that has a devastating impact on health. The objective of this study was to describe the prevalence of and factors influencing smoking among medical and non-medical students in Tbilisi, Georgia, as well as to determine whether medical education has an impact on smoking. METHODS: A cross-sectional study was carried out at Tbilisi State Medical University and Tbilisi State University, both of which are located in Tbilisi, Georgia. A total of 400 4th-year students (200 students at each university) were asked to complete standardized questionnaires. RESULTS: Of the sample as a whole, 48.75% were identified as smokers and 51.25% were identified as nonsmokers. The mean age was 20.24 years among smokers and 20.26 years among nonsmokers. Of the medical students, 49.5% were smokers, as were 48.0% of the non-medical students. The male-to-female ratio in the study population was 0.9:1.1. Smoking was found to have a strong relationship with gender, males accounting for 65% of all smokers. Of the smokers, 56.9% stated that they would like to quit smoking (for health or financial reasons). Of the medical students, 59.5% expressed a willingness to quit smoking, as did 54.2% of the non-medical students. CONCLUSIONS: There is a need to improve smoking education for undergraduate students. Special attention should be given to the inclusion of anti-smoking education in undergraduate curricula, as well as to the implementation of smoking prevention campaigns at institutions of higher education. However, such measures will be effective only if tobacco control policies are strictly enforced on the national level as well.
Descriptors
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Book Title
Database
Publisher
Data Source
Authors
Chkhaidze,I., Maglakelidze,N., Maglakelidze,T., Khaltaev,N.
Original/Translated Title
URL
Date of Electronic
PMCID
PMC4075884
Editors
Cigarette and waterpipe smoking associated knowledge and behaviour among medical students in Lebanon 2013 College of Public Health and Health Informatics, King Saud Bin Abdulaziz University for Health Sciences, National Guard Health Affairs, King Abdulaziz Medical City, Riyadh, Saudi Arabia. jradiho@ngha.med.sa
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit
Periodical, Abbrev.
East.Mediterr.Health J.
Pub Date Free Form
Oct
Volume
19
Issue
10
Start Page
861
Other Pages
868
Notes
JID: 9608387; ppublish
Place of Publication
Egypt
ISSN/ISBN
1020-3397; 1020-3397
Accession Number
PMID: 24313150
Language
eng
SubFile
Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
24313150
Abstract
As future physicians capable of controlling tobacco dependence in the population, medical students are considered a main target for tobacco control interventions. This cross-sectional study reported on the prevalence of tobacco use (cigarettes and waterpipes) and associated knowledge and behaviour among 6th-year medical students in 2009-2010 from 6 medical schools in Lebanon. The self-administered questionnaire based on the Global Health Professional Survey (GHPSS) core questions also enquired about training in tobacco cessation approaches. All enrolled students were asked to participate; the response rate was 191/354 (54.3%). The prevalence of tobacco use was 26.3% for cigarettes and 29.5% for waterpipes. Smoking waterpipes was the only significant predictor for cigarette smoking and there was no difference by sex and socioeconomic status. A minority reported ever receiving any formal training in treatment approaches for tobacco dependence. Medical schools should include tobacco dependence treatment training programmes in their curriculum and discourage tobacco use.
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Database
Publisher
Data Source
Authors
Jradi,H., Wewers,M.E., Pirie,P.R., Binkley,P.F., Ferketich,K.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Understanding tobacco-related attitudes among college and noncollege young adult hookah and cigarette users 2014 a Public Health Research Division , RTI International , Research Triangle Park , North Carolina.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of American college health : J of ACH
Periodical, Abbrev.
J.Am.Coll.Health
Pub Date Free Form
Volume
62
Issue
1
Start Page
10
Other Pages
18
Notes
LR: 20150422; GR: R01 CA087472/CA/NCI NIH HHS/United States; GR: R01-CA87472/CA/NCI NIH HHS/United States; GR: R25 CA113710/CA/NCI NIH HHS/United States; GR: R25T-113710/PHS HHS/United States; GR: U01 CA154240/CA/NCI NIH HHS/United States; GR: U01-CA15424
Place of Publication
United States
ISSN/ISBN
1940-3208; 0744-8481
Accession Number
PMID: 24313692
Language
eng
SubFile
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; IM
DOI
10.1080/07448481.2013.842171 [doi]
Output Language
Unknown(0)
PMID
24313692
Abstract
OBJECTIVE: To examine differences in tobacco-related attitudes and hookah and cigarette use among college and noncollege young adults. PARTICIPANTS: Time-location samples of young adult bar patrons in San Diego, California (N = 2,243), Tulsa (N = 2,095) and Oklahoma City (N = 2,200), Oklahoma, Albuquerque (N = 1,044) and Las Cruces (N = 894), New Mexico, between September 2009 and July 2011. METHODS: Multinomial logistic regression examined associations between hookah and cigarette use and tobacco-related attitudes. RESULTS: Current college students and graduates are less likely to smoke cigarettes, but more likely to use hookah. Among current hookah users, 22.6% were hookah-only users and 77.4% were dual users (cigarettes and hookah). College status is associated with different hookah use patterns, and those with anti-tobacco industry attitudes were more likely to smoke hookah. CONCLUSIONS: Novel interventions are needed for college students using hookah. Existing strategies targeting smokers with anti-tobacco industry messages may be irrelevant to hookah users.
Descriptors
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Book Title
Database
Publisher
Data Source
Authors
Lee,Y.O., Bahreinifar,S., Ling,P.M.
Original/Translated Title
URL
Date of Electronic
PMCID
PMC3920485
Editors