WTS database

Title	Pub Year	Author Sort descending	SearchLink
First description of CTX-M beta-lactamase-producing clinical Escherichia coli isolates from Egypt	2006	Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full International journal of antimicrobial agents Periodical, Abbrev. Int.J.Antimicrob.Agents Pub Date Free Form Jun Volume 27 Issue 6 Start Page 545 Other Pages 548 Notes JID: 9111860; 0 (Escherichia coli Proteins); EC 3.5.2.- (CTX-M-27, E coli); EC 3.5.2.- (beta-lactamase CTX-M-14); EC 3.5.2.- (beta-lactamase CTX-M-15); EC 3.5.2.6 (beta-Lactamases); 2005/11/02 [received]; 2006/01/08 [accepted]; 2006/05/18 [aheadofprint]; Place of Publication Netherlands ISSN/ISBN 0924-8579; 0924-8579 Accession Number PMID: 16713187 Language eng SubFile Journal Article; IM DOI S0924-8579(06)00092-6 [pii] Output Language Unknown(0) PMID 16713187 Abstract We studied the presence of beta-lactamases with an extended spectrum of activity in clinical Escherichia coli isolates from Cairo, Egypt. Forty-six E. coli isolates were collected from patients with urinary tract infections at a university hospital in 2001. Phenotypic characterisation identified a very high extended-spectrum beta-lactamase (ESBL) rate of 60.9%. Pulsed-field gel electrophoresis and plasmid profiles revealed eight different clonal groups. All ESBL producers were polymerase chain reaction-positive for blaTEM and blaCTX-M genes. Within the CTX-M family, three different enzymes, CTX-M-14, CTX-M-15 and CTX-M-27, were found. The ESBL producers carried multiple plasmids and further plasmid-encoded resistances. In several strains, genes for up to six aminoglycoside-modifying enzymes were detected. A linkage to fluoroquinolone resistance was not observed. This study confirms the high rate of ESBLs in Egypt and further demonstrates the worldwide spread of genes coding for CTX-M enzymes in clinical isolates. Descriptors Drug Resistance, Bacterial, Escherichia coli/drug effects/enzymology, Escherichia coli Proteins/genetics, Humans, Plasmids, Polymerase Chain Reaction, beta-Lactamases/genetics Links Book Title Database Publisher Data Source Authors Mohamed Al-Agamy,M. H., El-Din Ashour,M. S., Wiegand,I. Original/Translated Title URL Date of Electronic 20060518 PMCID Editors
Role for cell density in antifungal drug resistance in Candida albicans biofilms	2007	Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Antimicrobial Agents and Chemotherapy Periodical, Abbrev. Antimicrob.Agents Chemother. Pub Date Free Form Jul Volume 51 Issue 7 Start Page 2454 Other Pages 2463 Notes LR: 20140907; GR: 1R01 DE14029/DE/NIDCR NIH HHS/United States; JID: 0315061; 0 (Antifungal Agents); 0 (Fungal Proteins); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); OID: NLM: PMC1913227; 2007/05/14 [aheadofprint]; ppublish Place of Publication United States ISSN/ISBN 0066-4804; 0066-4804 Accession Number PMID: 17502416 Language eng SubFile Journal Article; Research Support, N.I.H., Extramural; IM DOI AAC.01237-06 [pii] Output Language Unknown(0) PMID 17502416 Abstract Biofilms of Candida albicans are less susceptible to many antifungal drugs than are planktonic yeast cells. We investigated the contribution of cell density to biofilm phenotypic resistance. Planktonic yeast cells in RPMI 1640 were susceptible to azole-class drugs, amphotericin B, and caspofungin at 1 x 10(3) cells/ml (standard conditions) using the XTT [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide sodium salt] assay. As reported by others, as the cell concentration increased to 1 x 10(8) cells/ml, resistance was observed with 10- to 20-fold-greater MICs. Biofilms that formed in microtiter plate wells, like high-density planktonic organisms, were resistant to drugs. When biofilms were resuspended before testing, phenotypic resistance remained, but organisms, when diluted to 1 x 10(3) cells/ml, were susceptible. Drug-containing medium recovered from high-cell-density tests inhibited low-cell-density organisms. A fluconazole-resistant strain showed greater resistance at high planktonic cell density, in biofilm, and in resuspended biofilm than did low-density planktonic or biofilm organisms. A strain lacking drug efflux pumps CDR1, CDR2, and MDR1, while susceptible at a low azole concentration, was resistant at high cell density and in biofilm. A strain lacking CHK1 that fails to respond to the quorum-sensing molecule farnesol had the same response as did the wild type. FK506, reported to abrogate tolerance to azole drugs at low cell density, had no effect on tolerance at high cell density and in biofilm. These observations suggested that cell density has a role in the phenotypic resistance of biofilm, that neither the drug efflux pumps tested nor quorum sensing through Chk1p contributes to resistance, and that azole drug tolerance at high cell density differs mechanistically from tolerance at low cell density. Descriptors Amphotericin B/pharmacology, Antifungal Agents/pharmacology, Biofilms/drug effects/growth & development, Candida albicans/drug effects/physiology, Drug Resistance, Fungal/genetics, Fluconazole/pharmacology, Fungal Proteins/physiology, Microbial Sensitivity Tests Links Book Title Database Publisher Data Source Authors Perumal,P., Mekala,S., Chaffin,W. L. Original/Translated Title URL Date of Electronic 20070514 PMCID PMC1913227 Editors
Prevalence of severe esophagitis in Spain. Results of the PRESS study (Prevalence and Risk factors for Esophagitis in Spain: A cross-sectional study)	2016	Department of Microbiology and Parasitology, Pharmacy Faculty, Universitat de Barcelona, Spain.; Department of Gastroenterology, Hospital Universitario La Fe, Valencia, Spain.; Department of Gastroenterology, Hospital Universitario La Fe, Valencia, Spain.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full United European gastroenterology journal Periodical, Abbrev. United.European Gastroenterol.J. Pub Date Free Form Apr Volume 4 Issue 2 Start Page 229 Other Pages 235 Notes LR: 20160420; JID: 101606807; OID: NLM: PMC4804364; OTO: NOTNLM; 2015/04/16 [received]; 2015/06/21 [accepted]; 2015/07/24 [epublish]; ppublish Place of Publication England ISSN/ISBN 2050-6406; 2050-6406 Accession Number PMID: 27087951 Language eng SubFile Journal Article DOI 10.1177/2050640615595916 [doi] Output Language Unknown(0) PMID 27087951 Abstract BACKGROUND: N.P. and M.P. contributed equally to this study.The current prevalence of esophagitis in southern Europe is unknown. In addition, the risk factors for reflux esophagitis are not fully understood. OBJECTIVE: The objective of this article is to assess the prevalence and risk factors for esophagitis in Spain. METHODS: A prospective, observational, cross-sectional, multicenter study (PRESS study) was conducted among 31 gastrointestinal endoscopy units throughout Spain. A total of 1361 patients undergoing upper gastrointestinal endoscopy were enrolled. Sociodemographic, clinical and treatment data were recorded. RESULTS: A total of 95% of patients were Caucasian and 52% were male (mean age: 53 +/- 17 years). The most frequent symptoms prompting endoscopy were heartburn (40%), regurgitation (26%) and dysphagia (15%). Fifty-four percent of patients undergoing endoscopy were receiving proton pump inhibitor (PPI) treatment. Esophagitis (mainly mild-moderate) was present in 154 (12.4%) patients. The severe form was recorded in only 11 (0.8%) patients. Multivariate analysis results indicated that the likelihood of esophagitis was higher in men (OR = 1.91, 95% CI = 1.31-2.78), in patients with high GERD-Q scores (OR = 1.256, 95% CI = 1.176-1.343), weight increase (OR = 1.014, 95% CI = 1.003-1.025) and high alcohol consumption (OR = 2.49, 95% CI = 1.16-5.36). CONCLUSION: Severe esophagitis is a rare finding in the Spanish population. Male gender, high GERD-Q score, weight increase and high alcohol consumption are main risk factors for its appearance. Descriptors* Links Book Title Database Publisher Data Source Authors Pique,N., Ponce,M., Garrigues,V., Rodrigo,L., Calvo,F., de Argila,C.M., Borda,F., Naranjo,A., Alcedo,J., Jose Soria,M., Rey,E., Bujanda,L., Gisbert,J.P., Suarez,D., Calvet,X., Ponce,J., PRESS Study Group Original/Translated Title URL Date of Electronic 20150724 PMCID PMC4804364 Editors
Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms	2001	Department of Microbiology, Division of Infectious Diseases, The University of Texas Health Science Center at San Antonio, Texas 78245, USA.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Antimicrobial Agents and Chemotherapy Periodical, Abbrev. Antimicrob.Agents Chemother. Pub Date Free Form Sep Volume 45 Issue 9 Start Page 2475 Other Pages 2479 Notes LR: 20140613; JID: 0315061; 0 (Antifungal Agents); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); OID: NLM: PMC90680; ppublish Place of Publication United States ISSN/ISBN 0066-4804; 0066-4804 Accession Number PMID: 11502517 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI Output Language Unknown(0) PMID 11502517 Abstract Candida albicans is implicated in many biomaterial-related infections. Typically, these infections are associated with biofilm formation. Cells in biofilms display phenotypic traits that are dramatically different from those of their free-floating planktonic counterparts and are notoriously resistant to antimicrobial agents. Consequently, biofilm-related infections are inherently difficult to treat and to fully eradicate with normal treatment regimens. Here, we report a rapid and highly reproducible microtiter-based colorimetric assay for the susceptibility testing of fungal biofilms, based on the measurement of metabolic activities of the sessile cells by using a formazan salt reduction assay. The assay was used for in vitro antifungal susceptibility testing of several C. albicans strains grown as biofilms against amphotericin B and fluconazole and the increased resistance of C. albicans biofilms against these antifungal agents was demonstrated. Because of its simplicity, compatibility with a widely available 96-well microplate platform, high throughput, and automation potential, we believe this assay represents a promising tool for the standardization of in vitro antifungal susceptibility testing of fungal biofilms. Descriptors Amphotericin B/pharmacology, Antifungal Agents/pharmacology, Biofilms/drug effects, Candida albicans/drug effects, Cell Count, Colorimetry, Fluconazole/pharmacology, Humans, Microbial Sensitivity Tests/standards, Reproducibility of Results Links Book Title Database Publisher Data Source Authors Ramage,G., Vande Walle,K., Wickes,B. L., Lopez-Ribot,J. L. Original/Translated Title URL Date of Electronic PMCID PMC90680 Editors
Importance of biofilm in Candida parapsilosis and evaluation of its susceptibility to antifungal agents by colorimetric method	2007	Department of Microbiology, Faculty of Medicine, Masaryk University, 601 77 Brno, Czechia. filip.ruzicka@fnusa.cz	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Folia microbiologica Periodical, Abbrev. Folia Microbiol.(Praha) Pub Date Free Form Volume 52 Issue 3 Start Page 209 Other Pages 214 Notes JID: 0376757; 0 (Antifungal Agents); ppublish Place of Publication Czech Republic ISSN/ISBN 0015-5632; 0015-5632 Accession Number PMID: 17702457 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI Output Language Unknown(0) PMID 17702457 Abstract The ability of C. parapsilosis (an important cause of nosocomial infections) to produce biofilm was evaluated in 32 bloodstream isolates and 85 strains isolated from skin. The biofilm formation was found in 19 (59%) blood isolates and only in 33 (39%) isolates from skin. The antifungal susceptibility was assessed for amphotericin B, itraconazole and voriconazole in planktonic and biofilm form of the 19 biofilm-positive bloodstream strains by broth microdilution method according to NCCLS standards. The method was modified by the use of resazurin as a colorimetric indicator of the metabolically active cells which makes the determination of the effect of antifungal agents easier. Biofilm forms of all strains were more resistant than their planktonic form. Descriptors Antifungal Agents/pharmacology, Biofilms/drug effects, Candida/drug effects/pathogenicity, Colorimetry, Cross Infection/microbiology, Drug Resistance, Fungal/drug effects, Fungemia/drug therapy, Humans, Microbial Sensitivity Tests/methods, Microbiological Techniques Links Book Title Database Publisher Data Source Authors Ruzicka,F., Hola,V., Votava,M., Tejkalova,R. Original/Translated Title URL Date of Electronic PMCID Editors
In vitro effect of amphotericin B on Candida albicans, Candida glabrata and Candida parapsilosis biofilm formation	2014	Department of Microbiology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 9 Maria Sklodowska-Curie Street, 85-094, Bydgoszcz, Poland, malgorzata_szabelska@wp.pl.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Mycopathologia Periodical, Abbrev. Mycopathologia Pub Date Free Form Feb Volume 177 Issue 2-Jan Start Page 19 Other Pages 27 Notes JID: 7505689; 0 (Antifungal Agents); 7XU7A7DROE (Amphotericin B); 2013/08/02 [received]; 2014/01/06 [accepted]; 2014/01/17 [aheadofprint]; ppublish Place of Publication Netherlands ISSN/ISBN 1573-0832; 0301-486X Accession Number PMID: 24436013 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1007/s11046-014-9727-7 [doi] Output Language Unknown(0) PMID 24436013 Abstract Candida spp. biofilm is considered highly resistant to conventional antifungals. The aim of this study was to investigate the in vitro effect of amphotericin B on Candida spp. biofilms at different stages of maturation. We investigated the activity of amphotericin B against 78 clinical isolates of Candida spp., representing three species, growing as planktonic and sessile cells, by a widely accepted broth microdilution method. The in vitro effect on sessile cell viability was evaluated by MTT reduction assay. All examined strains were susceptible to amphotericin B when grown as free-living cells. At the early stages of biofilm maturation 96.7-100.0 % strains, depending on species, displayed amphotericin B sessile minimal inhibitory concentration (SMIC) = 1 mug/mL. Mature Candida spp. biofilm of 32.1-90.0 % strains displayed amphotericin B SMIC = 1 mug/mL. Based on these results, amphotericin B displays species- and strain-depending activity against Candida spp. biofilms. Descriptors Links Book Title Database Publisher Data Source Authors Prazynska,M., Gospodarek,E. Original/Translated Title URL Date of Electronic 20140117 PMCID Editors
Prevalence of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in food-producing animals	2012	Department of Microbiology, Shizuoka Institute of Environment and Hygiene, 4-27-2 Kitaando, Aoi, Shizuoka 420-8637, Japan. midori1_hiroi@pref.shizuoka.lg.jp	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full The Journal of veterinary medical science / the Japanese Society of Veterinary Science Periodical, Abbrev. J.Vet.Med.Sci. Pub Date Free Form Feb Volume 74 Issue 2 Start Page 189 Other Pages 195 Notes JID: 9105360; 0 (DNA, Bacterial); EC 3.5.2.- (beta-lactamase CTX-M-14); EC 3.5.2.6 (beta-Lactamases); 2011/10/07 [aheadofprint]; ppublish Place of Publication Japan ISSN/ISBN 1347-7439; 0916-7250 Accession Number PMID: 21979457 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI JST.JSTAGE/jvms/11-0372 [pii] Output Language Unknown(0) PMID 21979457 Abstract To evaluate the diversity of extended-spectrum beta-lactamases (ESBL) genes among food-producing animals, 48 isolates of ESBL-producing Escherichia coli isolates were obtained from rectal samples of broilers, layers, beef cattle and pigs, at the slaughterhouse level. ESBL-carrying E. coli were isolated from 60.0% of individual broiler rectal samples, 5.9% of layers, 12.5% of beef cattle and 3% of pigs. One ESBL-producing Klebsiella pneumoniae was isolated from a broiler. The ESBL-positive E. coli isolates from broilers harbored various ESBL genes: bla (SHV-12), bla(CTX-M-2), bla(CTX-M-14), bla(CTX-M-15) and bla(CTX-M-44). The plasmid DNAs were analyzed by restriction patterns. Homogeneous band patterns were yielded in those of K. pneumoniae and E. coli isolates harboring the bla(CTX-M-2) gene from different farms. No genetic relation between the 2 CTX-M-14 ESBL-producing strains was found by pulsed-field gel electrophoresis, although 2 plasmids in these strains, obtained from different broiler farms, were similar to each other. This study provides evidence that the proliferation of CTX-M-producing E. coli is due to the growth of indigenous CTX-M-producing strains and the possible emergence of strains that acquired CTX-M genes by horizontal transfer in different broiler farms. CTX-M-producing coliforms in broilers should be controlled due to the critical importance of cephalosporins and the zoonotic potential of ESBL-producing bacteria. Descriptors Links Book Title Database Publisher Data Source Authors Hiroi,M., Yamazaki,F., Harada,T., Takahashi,N., Iida,N., Noda,Y., Yagi,M., Nishio,T., Kanda,T., Kawamori,F., Sugiyama,K., Masuda,T., Hara-Kudo,Y., Ohashi,N. Original/Translated Title URL Date of Electronic 20111007 PMCID Editors
Microbial, saccharifying and antioxidant properties of an Indian rice based fermented beverage	2015	Department of Microbiology, Vidyasagar University, Midnapore 721102, West Bengal, India.; Department of Microbiology, Vidyasagar University, Midnapore 721102, West Bengal, India.; Department of Microbiology, Vidyasagar University, Midnapore 721102, West B	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Food Chemistry Periodical, Abbrev. Food Chem. Pub Date Free Form 1-Feb Volume 168 Issue Start Page 196 Other Pages 202 Notes CI: Copyright (c) 2014; JID: 7702639; 0 (Antioxidants); OTO: NOTNLM; 2014/04/12 [received]; 2014/06/26 [revised]; 2014/07/07 [accepted]; 2014/07/16 [aheadofprint]; ppublish Place of Publication England ISSN/ISBN 0308-8146; 0308-8146 Accession Number PMID: 25172700 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1016/j.foodchem.2014.07.042 [doi] Output Language Unknown(0) PMID 25172700 Abstract Haria, a popular rice based ethnic fermented beverage, is consumed as a staple food and refreshing drink by the vast number of Indian tribal people. In this study, the composition of microbial consortia and the occurrence of some important nutraceuticals during haria preparation were investigated. The quantities of moulds and yeasts were highest at 2nd day, and then declined, but, on the contrary, the quantity of Lactic Acid Bacteria and Bifidobacterium sp. increased concurrently during the course of fermentation. Accumulation of starch hydrolytic enzymes along with different types of malto-oligosaccharides like maltotetrose (26.18mug/gm), maltotriose (28.16mug/gm), and maltose (26.94mug/gm) were also noted. Furthermore, GC-MS analysis indicated the occurrence of pyranose derivatives in the fermented products. The fermented materials showed higher free radicals scavenging activity (82.54%, 4th day) against DPPH radicals. These studies clearly demonstrated that the microbial interaction during fermentation of rice makes it more nutritious, and most likely more beneficial for health. Descriptors Links Book Title Database Publisher Elsevier Ltd Data Source Authors Ghosh,K., Ray,M., Adak,A., Dey,P., Halder,S.K., Das,A., Jana,A., Parua Mondal,S., Das Mohapatra,P.K., Pati,B.R., Mondal,K.C. Original/Translated Title URL Date of Electronic 20140716 PMCID Editors
Effect of plasmids harbouring blaCTX-M on the virulence and fitness of Escherichia coli ST131 isolates	2015	Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, South Korea.; Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University Scho	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full International journal of antimicrobial agents Periodical, Abbrev. Int.J.Antimicrob.Agents Pub Date Free Form Aug Volume 46 Issue 2 Start Page 214 Other Pages 218 Notes CI: Copyright (c) 2015; JID: 9111860; EC 3.5.2.- (beta-lactamase CTX-M-14); EC 3.5.2.- (beta-lactamase CTX-M-15); EC 3.5.2.6 (beta-Lactamases); OTO: NOTNLM; 2014/11/27 [received]; 2015/04/23 [revised]; 2015/04/26 [accepted]; 2015/06/04 [aheadofprint]; ppu Place of Publication Netherlands ISSN/ISBN 1872-7913; 0924-8579 Accession Number PMID: 26116415 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1016/j.ijantimicag.2015.04.012 [doi] Output Language Unknown(0) PMID 26116415 Abstract The effect of plasmids harbouring blaCTX-M on the virulence and fitness on Escherichia coli sequence type 131 (ST131) isolates was investigated. Plasmids harbouring blaCTX-M-14 or blaCTX-M-15 were transferred by transconjugation into five non-extended-spectrum beta-lactamase (ESBL)-producing ST131 isolates. Clinical non-ESBL-producing ST131 isolates demonstrated a higher degree of biofilm formation and serum resistance compared with CTX-M-producing ST131 isolates. In addition, non-ESBL-producing isolates were more competitive than CTX-M-producing isolates. Transconjugants showed no significant defect in growth rate and competitiveness compared with their hosts. However, serum resistance and biofilm formation were diminished in the transconjugants. In conclusion, non-ESBL-producing E. coli ST131 isolates were more competitive and virulent than CTX-M-producing E. coli ST131 isolates. However, transconjugants harbouring blaCTX-M were no less competitive than their susceptible hosts, which may partially explain the global dissemination of CTX-M-14- and CTX-M-15-producing E. coli ST131 isolates, in addition to their increased antimicrobial resistance. Descriptors Links Book Title Database Publisher Elsevier B.V. and the International Society of Chemotherapy Data Source Authors Shin,J., Ko,K.S. Original/Translated Title URL Date of Electronic 20150604 PMCID Editors
In vitro evaluation of antibiotic lock technique for the treatment of Candida albicans, C. glabrata, and C. tropicalis biofilms	2010	Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Korea.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Journal of Korean medical science Periodical, Abbrev. J.Korean Med.Sci. Pub Date Free Form Dec Volume 25 Issue 12 Start Page 1722 Other Pages 1726 Notes LR: 20150205; JID: 8703518; 0 (Antifungal Agents); 0 (Echinocandins); 0 (Pyrimidines); 0 (Triazoles); 304NUG5GF4 (Itraconazole); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); F0XDI6ZL63 (caspofungin); JFU09I87TR (Voriconazole); OID: NLM: PMC29952 Place of Publication Korea (South) ISSN/ISBN 1598-6357; 1011-8934 Accession Number PMID: 21165285 Language eng SubFile Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.3346/jkms.2010.25.12.1722 [doi] Output Language Unknown(0) PMID 21165285 Abstract Candidaemia associated with intravascular catheter-associated infections is of great concern due to the resulting high morbidity and mortality. The antibiotic lock technique (ALT) was previously introduced to treat catheter-associated bacterial infections without removal of catheter. So far, the efficacy of ALT against Candida infections has not been rigorously evaluated. We investigated in vitro activity of ALT against Candida biofilms formed by C. albicans, C. glabrata, and C. tropicalis using five antifungal agents (caspofungin, amphotericin B, itraconazole, fluconazole, and voriconazole). The effectiveness of antifungal treatment was assayed by monitoring viable cell counts after exposure to 1 mg/mL solutions of each antibiotic. Fluconazole, itraconazole, and voriconazole eliminated detectable viability in the biofilms of all Candida species within 7, 10, and 14 days, respectively, while caspofungin and amphotericin B did not completely kill fungi in C. albicans and C. glabrata biofilms within 14 days. For C. tropicalis biofilm, caspofungin lock achieved eradication more rapidly than amphotericin B and three azoles. Our study suggests that azoles may be useful ALT agents in the treatment of catheter-related candidemia. Descriptors Amphotericin B/administration & dosage/pharmacology, Antifungal Agents/administration & dosage/pharmacology/therapeutic use, Biofilms/drug effects, Candida albicans/drug effects/physiology, Candida glabrata/drug effects/physiology, Candida tropicalis/drug effects/physiology, Candidiasis/drug therapy, Catheter-Related Infections/drug therapy, Catheterization, Central Venous, Drug Administration Routes, Echinocandins/administration & dosage/pharmacology, Fluconazole/administration & dosage/pharmacology, Humans, Itraconazole/administration & dosage/pharmacology, Microbial Sensitivity Tests, Pyrimidines/administration & dosage/pharmacology, Triazoles/administration & dosage/pharmacology, Voriconazole, Antibiotic Lock Technique, Biofilms, Candida Links Book Title Database Publisher Data Source Authors Ko,K. S., Lee,J. Y., Song,J. H., Peck,K. R. Original/Translated Title URL Date of Electronic 20101124 PMCID PMC2995224 Editors

E-mail