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waterpipe, water-pipe, nargile, narghile, arguileh, arguile, shisha, sheesha, chichi, hubble bubble, hubbly bubbly, goza, hookah, bong
| Title Sort descending | Pub Year | Author | SearchLink |
|---|---|---|---|
| In vitro activity of seven systemically active antifungal agents against a large global collection of rare Candida species as determined by CLSI broth microdilution methods | 2009 | Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, IA 52242, USA. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of clinical microbiology
Periodical, Abbrev.
J.Clin.Microbiol.
Pub Date Free Form
Oct
Volume
47
Issue
10
Start Page
3170
Other Pages
3177
Notes
LR: 20141207; JID: 7505564; 0 (Antifungal Agents); OID: NLM: PMC2756931; 2009/08/26 [aheadofprint]; ppublish
Place of Publication
United States
ISSN/ISBN
1098-660X; 0095-1137
Accession Number
PMID: 19710283
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1128/JCM.00942-09 [doi]
Output Language
Unknown(0)
PMID
19710283
Abstract
Five Candida species (C. albicans, C. glabrata, C. tropicalis, C. parapsilosis, and C. krusei) account for over 95% of invasive candidiasis cases. Some less common Candida species have emerged as causes of nosocomial candidiasis, but there is little information about their in vitro susceptibilities to antifungals. We determined the in vitro activities of fluconazole, voriconazole, posaconazole, amphotericin B, anidulafungin, caspofungin, and micafungin against invasive, unique patient isolates of Candida collected from 100 centers worldwide between January 2001 and December 2007. Antifungal susceptibility testing was performed by the CLSI M27-A3 method. CLSI breakpoints for susceptibility were used for fluconazole, voriconazole, anidulafungin, caspofungin, and micafungin, while a provisional susceptibility breakpoint of < or = 1 microg/ml was used for amphotericin and posaconazole. Of 14,007 Candida isolates tested, 658 (4.7%) were among the less common species. Against all 658 isolates combined, the activity of each agent, expressed as the MIC50/MIC90 ratio (and the percentage of susceptible isolates) was as follows: fluconazole, 1/4 (94.8%); voriconazole, 0.03/0.12 (98.6%); posaconazole, 0.12/0.5 (95.9%); amphotericin, 0.5/2 (88.3%); anidulafungin, 0.5/2 (97.4%); caspofungin, 0.12/0.5 (98.0%); and micafungin, 0.25/1 (99.2%). Among the isolates not susceptible to one or more of the echinocandins, most (68%) were C. guilliermondii. All isolates of the less common species within the C. parapsilosis complex (C. orthopsilosis and C. metapsilosis) were susceptible to voriconazole, posaconazole, anidulafungin, caspofungin, and micafungin. Over 95% of clinical isolates of the rare Candida species were susceptible to the available antifungals. However, activity did vary by drug-species combination, with some species (e.g., C. rugosa and C. guilliermondii) demonstrating reduced susceptibilities to commonly used agents such as fluconazole and echinocandins.
Descriptors
Antifungal Agents/pharmacology, Candida/drug effects/isolation & purification, Candidiasis/microbiology, Drug Resistance, Fungal, Humans, Microbial Sensitivity Tests
Links
Book Title
Database
Publisher
Data Source
Authors
Diekema,D. J., Messer,S. A., Boyken,L. B., Hollis,R. J., Kroeger,J., Tendolkar,S., Pfaller,M. A.
Original/Translated Title
URL
Date of Electronic
20090826
PMCID
PMC2756931
Editors
|
|||
| In vitro activity of xanthorrhizol against Candida glabrata, C. guilliermondii, and C. parapsilosis biofilms | 2011 | Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Medical mycology
Periodical, Abbrev.
Med.Mycol.
Pub Date Free Form
Jan
Volume
49
Issue
1
Start Page
1
Other Pages
9
Notes
LR: 20131213; JID: 9815835; 0 (Antifungal Agents); 0 (Phenols); 0 (Tetrazolium Salts); 0 (xanthorrhizol); 117038-70-7 (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazoliu m hydroxide); 2010/06/21 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
1460-2709; 1369-3786
Accession Number
PMID: 20560862
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.3109/13693786.2010.492482 [doi]
Output Language
Unknown(0)
PMID
20560862
Abstract
The formation of Candida biofilms has important clinical ramifications, because these biofilms exhibit increased resistance to conventional antifungal therapies. The aim of this study was to investigate the activity of xanthorrhizol on biofilms produced by non-C. albicans Candida (NCAC) species, including C. glabrata, C. guilliermondii, and C. parapsilosis. NCAC biofilms were generated in flat-bottom 96-well microtiter plates and quantified using the XTT (2, 3 - bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenyl amino) carbonyl]-2H-tetrazolium hydroxide) reduction assay. The NCAC biofilms at adherent, intermediate, and mature growth phases were treated with 0.5-512 mug/ml of xanthorrhizol for 24 h. The ranges of sessile minimum inhibitory concentrations (SMICs) of xanthorrhizol against C. glabrata, C. guilliermondii, and C. parapsilosis biofilms were 8-32 mug/ml, 8-16 mug/ml, and 8-64 mug/ml, respectively. Xanthorrhizol affected cell density that had an indirect effect on the biofilm OD(490). The compound eradicated the viable cells of the C. glabrata and C. parapsilosis biofilms at the adherent growth phase at 16 mug/ml and that of C. guilliermondii at 8 mug/ml. Treatment with 128 mug/ml of xanthorrhizol reduced the OD(490) of C. glabrata, C. guilliermondii, and C. parapsilosis biofilms at the mature growth phase by 77.8%, 88.5%, and 64.5%, respectively. These results indicate that xanthorrhizol exhibits potent activity against NCAC biofilms in vitro. Therefore, xanthorrhizol has potential therapeutic value in treating biofilm-associated NCAC infections and should be further evaluated in vivo.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Rukayadi,Y., Han,S., Yong,D., Hwang,J.K.
Original/Translated Title
URL
Date of Electronic
20100621
PMCID
Editors
|
|||
| In vitro analyses of the combination of high-dose doxycycline and antifungal agents against Candida albicans biofilms | 2009 | Division of Infectious Diseases, University of New Mexico Health Science Center, Albuquerque, NM 87108, USA. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International journal of antimicrobial agents
Periodical, Abbrev.
Int.J.Antimicrob.Agents
Pub Date Free Form
Oct
Volume
34
Issue
4
Start Page
326
Other Pages
332
Notes
LR: 20140603; GR: T32 AI007538/AI/NIAID NIH HHS/United States; JID: 9111860; 0 (Antifungal Agents); 0 (Echinocandins); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); F0XDI6ZL63 (caspofungin); N12000U13O (Doxycycline); 2009/01/18 [received]; 2009/0
Place of Publication
Netherlands
ISSN/ISBN
1872-7913; 0924-8579
Accession Number
PMID: 19515537
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; IM
DOI
10.1016/j.ijantimicag.2009.04.011 [doi]
Output Language
Unknown(0)
PMID
19515537
Abstract
The potential of antifungal agents used as antimicrobial lock therapy (ALT) for the conservative management of catheter-related candidemia has not been fully defined. We sought to determine the antifungal effect of high-dose doxycycline (DOX), alone or in combination with standard concentrations of amphotericin B (AMB), caspofungin (CAS) or fluconazole (FLC), against biofilms formed by Candida albicans in vitro. DOX alone (at 2048 microg/mL and 1024 microg/mL) demonstrated up to an 85% reduction of the metabolic activity of the C. albicans biofilm. Regardless of the concentration tested, FLC alone showed minimal activity (mean 22.9% reduction) against the C. albicans biofilm. When DOX 2048 microg/mL was used in combination with FLC, antifungal activity also increased up to 85%, suggesting an additive effect. DOX 128 microg/mL in combination with FLC demonstrated synergy (mean 58.3% reduction). The combination of DOX 2048 microg/mL or 512 microg/mL and AMB was superior to AMB alone at low concentrations (0.25-0.03125 microg/mL). However, DOX 128 microg/mL was antagonistic in combination with low concentrations of AMB. Maximal efficacy against the biofilm was observed with CAS at 8-0.25 microg/mL compared with FLC and AMB alone. A paradoxical effect (PE) occurred with CAS at 16 microg/mL, which showed a marked reduction in antifungal activity compared with lower concentrations of CAS. CAS at 16 microg/mL in combination with either DOX 2048 microg/mL or 512 microg/mL resulted in attenuation of the PE. These findings suggest that a high-dose DOX-based ALT strategy in combination with traditional antifungal agents may be useful for the treatment of C. albicans biofilms.
Descriptors
Amphotericin B/pharmacology, Antifungal Agents/pharmacology, Biofilms/drug effects, Candida albicans/drug effects, Dose-Response Relationship, Drug, Doxycycline/administration & dosage/pharmacology, Drug Interactions, Drug Therapy, Combination, Echinocandins/pharmacology, Fluconazole/pharmacology, Humans, Microbial Sensitivity Tests
Links
Book Title
Database
Publisher
Data Source
Authors
Miceli,M. H., Bernardo,S. M., Lee,S. A.
Original/Translated Title
URL
Date of Electronic
20090609
PMCID
Editors
|
|||
| In vitro antifungal susceptibilities of isolates of Candida spp. and Aspergillus spp. from China to nine systemically active antifungal agents: data from the SENTRY antifungal surveillance program, 2010 through 2012 | 2015 | University of Iowa College of Medicine, Iowa City, IA, USA; JMI Laboratories, North Liberty, IA, USA. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Mycoses
Periodical, Abbrev.
Mycoses
Pub Date Free Form
Apr
Volume
58
Issue
4
Start Page
209
Other Pages
214
Notes
CI: (c) 2015; JID: 8805008; 0 (Antifungal Agents); 0 (Azoles); 0 (Echinocandins); 0 (Triazoles); 6TK1G07BHZ (posaconazole); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); D83282DT06 (Flucytosine); JFU09I87TR (Voriconazole); OTO: NOTNLM; 2014/11/20
Place of Publication
Germany
ISSN/ISBN
1439-0507; 0933-7407
Accession Number
PMID: 25727853
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1111/myc.12299 [doi]
Output Language
Unknown(0)
PMID
25727853
Abstract
We report the in vitro activity of nine systemically active antifungal agents against 237 contemporary clinical isolates of yeast and moulds obtained from 13 laboratories in China during 2010 through 2012. Susceptibility testing was performed using CLSI methods. Sequencing of fks hot spots was performed for echinocandin non-wild-type (WT) strains. Isolates included 220 from eight species of Candida, 15 from four species of Aspergillus and one isolate each of Rhodotorula mucilaginosa and Trichosporon asahii. Resistance to amphotericin B (0.0%), flucytosine (0.0-1.7%) and the echinocandins (0.0-3.4%) was distinctly uncommon among C. albicans, C. parapsilosis, C. tropicalis, C. glabrata and C. pelliculosa. Three C. albicans isolates showed resistance to echinocandins and one harboured a mutation in HS1 of fks1. Resistance to the azoles was much more common with resistance to fluconazole, voriconazole and posaconazole detected among isolates of C. glabrata and C. tropicalis. Both C. parapsilosis and C. pelliculosa exhibited decreased susceptibility to fluconazole. Amphotericin B, the mould-active azoles and the echinocandins were all quite active against isolates of A. fumigatus and A. flavus. Consistent with previous studies from China, resistance to fluconazole is prominent among Candida spp. isolates in this country.
Descriptors
Links
Book Title
Database
Publisher
Blackwell Verlag GmbH
Data Source
Authors
Pfaller,M.A., Castanheira,M., Messer,S.A., Jones,R.N.
Original/Translated Title
URL
Date of Electronic
20150227
PMCID
Editors
|
|||
| In vitro biofilm characterization and activity of antifungal agents alone and in combination against sessile and planktonic clinical Candida albicans isolates | 2007 | Division of Infectious Diseases, Department of Medicine, Mayo Clinic College of Medicine, Rochester, MN 55905, USA. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Diagnostic microbiology and infectious disease
Periodical, Abbrev.
Diagn.Microbiol.Infect.Dis.
Pub Date Free Form
Mar
Volume
57
Issue
3
Start Page
277
Other Pages
281
Notes
LR: 20141120; JID: 8305899; 0 (Antifungal Agents); 0 (Echinocandins); 0 (Peptides, Cyclic); 0 (Pyrimidines); 0 (Triazoles); F0XDI6ZL63 (caspofungin); JFU09I87TR (Voriconazole); 2006/08/04 [received]; 2006/09/12 [revised]; 2006/09/15 [accepted]; 2006/12/01
Place of Publication
United States
ISSN/ISBN
0732-8893; 0732-8893
Accession Number
PMID: 17141454
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
S0732-8893(06)00370-1 [pii]
Output Language
Unknown(0)
PMID
17141454
Abstract
Thirty clinical isolates of Candida albicans were collected from blood or other sterile site infections. Biofilm dry weight and metabolic activity were measured for each isolate. Planktonic and sessile antifungal susceptibilities of each isolate were determined for amphotericin B deoxycholate, caspofungin, and voriconazole. Sessile susceptibilities were determined for the combination of caspofungin/voriconazole. No significant differences in biofilm dry weight or metabolic activity were found between bloodstream and other invasive isolates. Planktonic MIC90 values and sessile MIC90 (SMIC90) values were 0.25 and 2, 0.06 and >256, and 0.5 and 2 microg/mL for amphotericin, voriconazole, and caspofungin, respectively. The SMIC90 of the combination of caspofungin/voriconazole against sessile isolates was 0.5/2 microg/mL. Therefore, the source of invasive C. albicans clinical isolates did not affect in vitro biofilm formation. Susceptibility to antifungal agents decreased when C. albicans was associated with biofilm, and the combination of caspofungin/voriconazole did not appear to provide enhanced activity compared with caspofungin alone.
Descriptors
Antifungal Agents/pharmacology, Biofilms/drug effects, Candida albicans/drug effects/physiology, Drug Therapy, Combination, Echinocandins, Humans, Microbial Sensitivity Tests/methods, Peptides, Cyclic/pharmacology, Pyrimidines/pharmacology, Triazoles/pharmacology, Voriconazole
Links
Book Title
Database
Publisher
Data Source
Authors
Shuford,J. A., Piper,K. E., Steckelberg,J. M., Patel,R.
Original/Translated Title
URL
Date of Electronic
20061201
PMCID
Editors
|
|||
| In vitro biofilm formation and relationship with antifungal resistance of Candida spp. isolated from vaginal and intrauterine device string samples of women with vaginal complaints | 2011 | Kocaeli University Faculty of Medicine, Department of Medical Microbiology, Kocaeli, Turkey. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Mikrobiyoloji bulteni
Periodical, Abbrev.
Mikrobiyol.Bul.
Pub Date Free Form
Oct
Volume
45
Issue
4
Start Page
697
Other Pages
706
Notes
LR: 20131121; JID: 7503830; 0 (Antifungal Agents); 304NUG5GF4 (Itraconazole); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); ppublish
Place of Publication
Turkey
ISSN/ISBN
0374-9096; 0374-9096
Accession Number
PMID: 22090300
Language
tur
SubFile
English Abstract; Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
22090300
Abstract
Intrauterin device (IUD) application is a widely used effective, safe and economic method for family planning. However IUD use may cause certain changes in vaginal ecosystem and may disturb microflora leading to increased colonization of various opportunistic pathogen microorganisms. The aims of this study were (i) to detect the biofilm production characteristics of Candida spp. isolated from vaginal and IUD string samples of women with IUDs, and (ii) to investigate the relationship between biofilm production and antifungal resistance. A total of 250 women (mean age: 34.4 +/- 7.6 years) admitted to gynecology outpatient clinics with vaginal symptoms (discharge and itching) were included in the study. The patients have been implanted CuT380a type IUDs for a mean duration of 59.8 +/- 42.4 months. Without removing IUD, string samples were obtained by cutting and simultaneous vaginal swab samples were also collected. Isolated Candida spp. were identified by conventional methods and API 20C AUX (BioMerieux, Fransa) system. Minimal inhibitory concentrations (MIC) of fluconazole, itraconazole and amphotericin B were determined by broth microdilution method according to the CLSI guidelines. Biofilm formation was evaluated by crystal violet staining and XTT-reduction assays, and the isolates which yielded positive results in both of the methods were accepted as biofilm-producers. In the study, Candida spp. were isolated from 33.2% (83/250) of the vaginal and 34% (85/250) of the IUD string samples, C.albicans being the most frequently detected species (54 and 66 strains for the samples, respectively). The total in vitro biofilm formation rate was 25% (21/83) for vaginal isolates and 44.7% (38/85) for IUD string isolates. Biofilm formation rate of vaginal C.albicans isolates was significantly lower than vaginal non-albicans Candida spp. (14.8% and 44.8%, respectively; p= 0.003). Biofilm formation rate of C.albicans strains isolated from vaginal and IUD string samples were found as 14.8% (8/54) and 45.5% (30/66), with a statistically significant importance (p 0.05]. Fluconazole resistance was significantly higher in biofilm-producing vaginal Candida spp. than those of nonproducers (52.4% vs. 16.1%; p= 0.001), however, itraconazole resistance was found similar in biofilmproducer and non-producer isolates (47.6% vs. 32.3%; p> 0.05). Resistance rates for both fluconazole and itraconazole were higher in biofilm-producers (39.5% and 52.6%, respectively), than those of non-producers (10.6% and 29.8%, respectively), representing a statistical significance (p= 0.002 and p= 0.03, respectively) for Candida spp. strains isolated from IUD string samples. The overall resistance rates of C.albicans and non-albicans Candida spp. against fluconazole, were determined as 15% and 54.2%, respectively, while those rates were 24.2% and 68.7%, respectively, against itraconazole. MIC value of amphotericin B for all of the Candida spp. isolates was = 1.5 microg/ml. In conclusion, the data obtained from this study revealed that Candida spp. May lead to vaginal infections by inducing biofilm formation in IUD strings and these biofilms may be related to resistance to antifungal agents. Thus, women using IUDs should be followed-up periodically for the development of biofilms in their IUD strings.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Caliskan,S., Keceli Ozcan,S., Cinar,S., Corakci,A., Caliskan,E.
Original/Translated Title
Vajinal Yakinmasi Olan Kadinlarin Vajen ve Rahim Ici Arac Ipi Orneklerinden Izole Edilen Candida Turlerinin In Vitro Biyofilm Olusturma Ozellikleri ve Antifungal Direnc ile Iliskisi
URL
Date of Electronic
PMCID
Editors
|
|||
| In vitro cytotoxicity and mutagenicity of mainstream waterpipe smoke and its functional consequences on alveolar type II derived cells | 2012 | Read more... | |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Toxicol Lett
Periodical, Abbrev.
Toxicol.Lett.
Pub Date Free Form
Volume
211
Issue
3
Start Page
220
Other Pages
31
Notes
ID: 22516759
Place of Publication
ISSN/ISBN
Accession Number
Language
en
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
INTRODUCTION: While waterpipe tobacco smoking has become a global phenomenon, its potential health consequences are poorly understood. In this manuscript, we report the in vitro mutagenicity of waterpipe smoke condensate (WSC), the alteration in cellular parameters of lung alveolar cells in response to WSC exposure and discuss the implication of cellular responses in the pathophysiology of chronic obstructive pulmonary disease (COPD). METHODS: The mainstream WSC was generated using a standard laboratory machine protocol. We assessed its mutagenicity using Ames test. In addition, we studied the effect of WSC on the proliferation and cell cycle of alveolar type II cells and vascular endothelial cells. We also assessed the effect of WSC on the expression of genes involved in cell cycle arrest and inflammation. RESULTS: Within the range of tested doses, WSC did not elicit sufficient response to be considered mutagenic in any of the strains tested (TA98, TA100, TA102, and TA97a) but were found to be toxic for strains TA97a and TA102 at the highest tested doses. However, WSC induced cell cycle arrest and cellular senescence mediated by the p53-p21 pathway. Also our study indicated that WSC induced an increase in the transcriptional expression of matrix metalloproteinases, MMP-2 and MMP-9 and an immune response regulator, Toll Like Receptor-4. CONCLUSION: The data reported here represent the first in vitro demonstration of the effect of waterpipe smoke on cellular parameters providing evidence of the potential involvement of WPS in the pathogenesis of COPD through impairing cellular growth and inducing inflammation.
Descriptors
Links
Book Title
Database
MEDLINE; http://www.globalhealthlibrary.net/
Publisher
Data Source
Authors
Rammah,Mayyasa, Dandachi,Farah, Salman,Rola, Shihadeh,Alan, El-Sabban, Marwan
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
|
|||
| In vitro effect of amphotericin B on Candida albicans, Candida glabrata and Candida parapsilosis biofilm formation | 2014 | Department of Microbiology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 9 Maria Sklodowska-Curie Street, 85-094, Bydgoszcz, Poland, malgorzata_szabelska@wp.pl. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Mycopathologia
Periodical, Abbrev.
Mycopathologia
Pub Date Free Form
Feb
Volume
177
Issue
2-Jan
Start Page
19
Other Pages
27
Notes
JID: 7505689; 0 (Antifungal Agents); 7XU7A7DROE (Amphotericin B); 2013/08/02 [received]; 2014/01/06 [accepted]; 2014/01/17 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
1573-0832; 0301-486X
Accession Number
PMID: 24436013
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1007/s11046-014-9727-7 [doi]
Output Language
Unknown(0)
PMID
24436013
Abstract
Candida spp. biofilm is considered highly resistant to conventional antifungals. The aim of this study was to investigate the in vitro effect of amphotericin B on Candida spp. biofilms at different stages of maturation. We investigated the activity of amphotericin B against 78 clinical isolates of Candida spp., representing three species, growing as planktonic and sessile cells, by a widely accepted broth microdilution method. The in vitro effect on sessile cell viability was evaluated by MTT reduction assay. All examined strains were susceptible to amphotericin B when grown as free-living cells. At the early stages of biofilm maturation 96.7-100.0 % strains, depending on species, displayed amphotericin B sessile minimal inhibitory concentration (SMIC) = 1 mug/mL. Mature Candida spp. biofilm of 32.1-90.0 % strains displayed amphotericin B SMIC = 1 mug/mL. Based on these results, amphotericin B displays species- and strain-depending activity against Candida spp. biofilms.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Prazynska,M., Gospodarek,E.
Original/Translated Title
URL
Date of Electronic
20140117
PMCID
Editors
|
|||
| In vitro effectiveness of anidulafungin against Candida sp. biofilms | 2013 | Dipartimento di Farmacia-Scienze del Farmaco, Universita degli Studi di Bari 'Aldo Moro', Via Orabona 4, Bari Italy.; Scuola di Dottorato e Scienze Biomediche e Oncologia umana, Sezione di Igiene, Universita di Bari 'Aldo Moro', Bari, Italy.; Scuola di Do | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
The Journal of antibiotics
Periodical, Abbrev.
J.Antibiot.(Tokyo)
Pub Date Free Form
Dec
Volume
66
Issue
12
Start Page
701
Other Pages
704
Notes
JID: 0151115; 0 (Antifungal Agents); 0 (Echinocandins); 9HLM53094I (anidulafungin); 2013/03/05 [received]; 2013/05/28 [revised]; 2013/07/30 [accepted]; 2013/09/11 [aheadofprint]; ppublish
Place of Publication
Japan
ISSN/ISBN
0021-8820; 0021-8820
Accession Number
PMID: 24022607
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1038/ja.2013.83 [doi]
Output Language
Unknown(0)
PMID
24022607
Abstract
This study furnishes deeper insights to previous works on anidulafungin, demonstrating the potent activity against Candida strains planktonic cells and biofilms. Candida sp., associated with many biomaterial-related infections, give rise to infective pathologies typically associated with biofilm formation. We recently determined the in vitro antifungal activities of echinocandin anidulafungin in association with some antifungal drugs against some Candida strains in their planktonic states. A total of 11 Candida strains biofilms were tested in this study: six Candida albicans, three C. parapsilosis and two C. tropicalis. All yeast isolates and ATCC strains were stored at -20 degrees C in glycerol stocks and were subcultured on antimicrobial agent-free Sabouraud dextrose agar plates. MIC endpoints were determined colorimetrically by using the indicator 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazoliu m hydroxide (XTT) with menadione as electron-coupling agent. The activity of anidulafungin was assessed using in vitro microbiological model relevant for clinical practice. Anidulafungin showed a strong activity in vitro against both planktonic and biofilms cells, and our study confirms that high anidulafungin concentrations might establish paradoxical growth effect in C. albicans and C. tropicalis biofilms.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Rosato,A., Piarulli,M., Schiavone,B.P., Catalano,A., Carocci,A., Carrieri,A., Carone,A., Caggiano,G., Franchini,C., Corbo,F., Montagna,M.T.
Original/Translated Title
URL
Date of Electronic
20130911
PMCID
Editors
|
|||
| In vitro effects of waterpipe smoke condensate on endothelial cell function: a potential risk factor for vascular disease | 2013 | Read more... | |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Toxicol Lett
Periodical, Abbrev.
Toxicol.Lett.
Pub Date Free Form
Volume
219
Issue
2
Start Page
133
Other Pages
42
Notes
ID: 23454654
Place of Publication
ISSN/ISBN
Accession Number
Language
en
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
AIM: Despite its increasing popularity, little is known about the health effects of waterpipe smoking (WPS), particularly on the cardiovascular system. To investigate the role of WPS as a risk factor for vascular disease, we evaluated its effect on endothelial cell function, which is an early event in vascular disease pathogenesis. We assessed the changes in cell viability, ROS generation, inflammatory and vasodilatory markers and in vitro angiogenesis of human aortic endothelial cells in response to waterpipe smoke condensate exposure. METHODS AND RESULTS: Mainstream waterpipe smoke condensate (WSC) was generated using a standard laboratory machine protocol. Compared to control, WSC induced cell cycle arrest, apoptosis, and oxidative stress in human primary endothelial cells. In addition, we assayed for impaired endothelium-dependent vasodilation and induced inflammation by studying the effect of WPS on the content and activity of AMPK, eNOS proteins and NF-κB p65 ser536 phosphorylation, respectively. WSC inhibited AMPK/eNOS phosphorylation and induced phosphorylation of p65. Moreover, we evaluated endothelial cells repair mechanism related properties that include migration/invasion and in vitro tube formation upon treatment with WSC. WSC reduced the motility and inhibited angiogenic potential of HAEC cells. CONCLUSIONS: WPS induced endothelial cell dysfunction as evident by exerting oxidative stress, inflammation, and impaired endothelial vasodilatory function and repair mechanisms. All together these data provide evidence for the potential contribution of WPS to endothelial dysfunction and thus to vascular disease.
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MEDLINE; http://www.globalhealthlibrary.net/
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Rammah,Mayyasa, Dandachi,Farah, Salman,Rola, Shihadeh,Alan, El-Sabban, Marwan
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