WTS database

Title Sort ascending	Pub Year	Author	SearchLink
Monitoring the lactic acid bacterial diversity during shochu fermentation by PCR-denaturing gradient gel electrophoresis	2005	Nodai Culture Collection Center, Department of Brewing, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan. 60020001@nodai.ac.jp	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Journal of bioscience and bioengineering Periodical, Abbrev. J.Biosci.Bioeng. Pub Date Free Form Mar Volume 99 Issue 3 Start Page 216 Other Pages 221 Notes LR: 20131121; JID: 100888800; 33X04XA5AT (Lactic Acid); 2004/09/15 [received]; 2004/11/29 [accepted]; ppublish Place of Publication Japan ISSN/ISBN 1389-1723; 1347-4421 Accession Number PMID: 16233780 Language eng SubFile Journal Article; IM DOI S1389-1723(05)70357-1 [pii] Output Language Unknown(0) PMID 16233780 Abstract The presence of lactic acid bacteria (LAB) during shochu fermentation was monitored by PCR-denaturing gradient gel electrophoresis (DGGE) and by bacteriological culturing. No LAB were detected from fermented mashes by PCR-DGGE using a universal bacterial PCR primer set. However, PCR-DGGE using a new primer specific for the 16S rDNA of Lactococcus, Streptococcus, Tetragenococcus, Enterococcus, and Vagococcus and two primers specific for the 16S rDNA of Lactobacillus, Pediococcus, Leuconostoc, and Weissella revealed that Enterococcus faecium, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus nagelii, Lactobacillus plantarum, Lactococcus lactis, Leuconostoc citreum, Leuconostoc mesenteroides, and Weissella cibaria inhabited in shochu mashes. It was also found that the LAB community composition during shochu fermentation changed after the main ingredient and water were added during the fermentation process. Therefore, we confirmed that PCR-DGGE using all three primers specific for groups of LAB together was well suited to the study of the LAB diversity in shochu mashes. The results of DGGE profiles were similar to the results of bacteriological culturing. In conclusion, LAB are present during shochu fermentation but not dominant. Descriptors Bacteria, Aerobic/genetics/isolation & purification/metabolism, Colony Count, Microbial/methods, Comet Assay/methods, Lactic Acid/metabolism, Nucleic Acid Denaturation, Polymerase Chain Reaction/methods, Wine/microbiology Links Book Title Database Publisher Data Source Authors Endo,A., Okada,S. Original/Translated Title URL Date of Electronic PMCID Editors
Monitoring polytobacco use among adolescents: do cigarette smokers use other forms of tobacco?	2008	Office on Smoking and Health, National Center for Chronic Disease and Health Promotion, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA. jbombard@cdc.gov	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco Periodical, Abbrev. Nicotine Tob.Res. Pub Date Free Form Nov Volume 10 Issue 11 Start Page 1581 Other Pages 1589 Notes LR: 20151119; JID: 9815751; ppublish Place of Publication England ISSN/ISBN 1462-2203; 1462-2203 Accession Number PMID: 18988070 Language eng SubFile Journal Article; IM DOI 10.1080/14622200802412887 [doi] Output Language Unknown(0) PMID 18988070 Abstract The extent of concurrent use of cigarettes and one or more other tobacco products (polytobacco use) is important to explore because users may be at an increased risk for adverse health effects and nicotine dependency. We determined national population estimates of current cigarette and current polytobacco use for at least 50,000 students from the 2002 and 2004 National Youth Tobacco Surveys. We identified which tobacco products were most often used in conjunction with cigarettes and used multivariate analyses to identify factors associated with polytobacco use. The overall prevalence was 16.0% for current cigarette smoking among all respondents and 15.0% for current cigarette smoking among respondents with complete information on concurrent cigarette and other tobacco product use: 8.1% used cigarettes only, and 6.9% were polytobacco users. Among current male cigarette smokers, 62.0% used other tobacco products; among current female cigarette smokers, 30.9% did. Among current cigarette smokers using one other tobacco product, cigars or smokeless tobacco were the most frequently used products. In multivariate analysis, polytobacco use was associated with being male; being in middle school; residing in the Midwest, South, or West; being able to obtain cigarettes from a retailer; being subject to peer influence; having favorable beliefs about tobacco; being willing to use tobacco promotional items; being exposed to tobacco advertisements; and having higher levels of lost autonomy (an indicator of nicotine dependency). Youth interventions need to broaden their focus to address the use of all tobacco products, paying particular attention to adolescent males and youth living outside of the Northeast. Descriptors Adolescent, Adolescent Behavior/psychology, Attitude to Health, Behavior, Addictive/epidemiology/psychology, Causality, Comorbidity, Female, Humans, Male, Peer Group, Product Labeling, Risk Factors, Risk-Taking, Self Concept, Sex Factors, Smoking/epidemiology/psychology, Smoking Cessation/statistics & numerical data, Surveys and Questionnaires, Tobacco Use Disorder/epidemiology/psychology, Tobacco, Smokeless, United States/epidemiology Links Book Title Database Publisher Data Source Authors Bombard,J. M., Rock,V. J., Pederson,L. L., Asman,K. J. Original/Translated Title URL Date of Electronic PMCID Editors
Monitoring Exposure to Ebola and Health of U.S. Military Personnel Deployed in Support of Ebola Control Efforts - Liberia, October 25, 2014-February 27, 2015	2015		Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full MMWR.Morbidity and mortality weekly report Periodical, Abbrev. MMWR Morb.Mortal.Wkly.Rep. Pub Date Free Form 3-Jul Volume 64 Issue 25 Start Page 690 Other Pages 694 Notes JID: 7802429; ppublish Place of Publication United States ISSN/ISBN 1545-861X; 0149-2195 Accession Number PMID: 26135589 Language eng SubFile Journal Article; IM DOI mm6425a2 [pii] Output Language Unknown(0) PMID 26135589 Abstract In response to the unprecedented Ebola virus disease (Ebola) outbreak in West Africa, the U.S. government deployed approximately 2,500 military personnel to support the government of Liberia. Their primary missions were to construct Ebola treatment units (ETUs), train health care workers to staff ETUs, and provide laboratory testing capacity for Ebola. Service members were explicitly prohibited from engaging in activities that could result in close contact with an Ebola-infected patient or coming in contact with the remains of persons who had died from unknown causes. Military units performed twice-daily monitoring of temperature and review of exposures and symptoms ("unit monitoring") on all persons throughout deployment, exit screening at the time of departure from Liberia, and post-deployment monitoring for 21 days at segregated, controlled monitoring areas on U.S. military installations. A total of 32 persons developed a fever during deployment from October 25, 2014, through February 27, 2015; none had a known Ebola exposure or developed Ebola infection. Monitoring of all deployed service members revealed no Ebola exposures or infections. Given their activity restrictions and comprehensive monitoring while deployed to Liberia, U.S. military personnel constitute a unique population with a lower risk for Ebola exposure compared with those working in the country without such measures. Descriptors Links Book Title Database Publisher Data Source Authors Cardile,A.P., Murray,C.K., Littell,C.T., Shah,N.J., Fandre,M.N., Drinkwater,D.C., Markelz,B.P., Vento,T.J. Original/Translated Title URL Date of Electronic PMCID Editors
Molecular typing of CTX-M-producing escherichia coli isolates from environmental water, swine feces, specimens from healthy humans, and human patients	2013	Second Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University, Hangzhou, China.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Applied and Environmental Microbiology Periodical, Abbrev. Appl.Environ.Microbiol. Pub Date Free Form Oct Volume 79 Issue 19 Start Page 5988 Other Pages 5996 Notes LR: 20150423; JID: 7605801; 0 (Anti-Bacterial Agents); 0 (Cephalosporins); 0 (DNA, Bacterial); 0 (Fluoroquinolones); EC 3.5.2.6 (beta-Lactamases); OID: NLM: PMC3811354; 2013/07/26 [aheadofprint]; ppublish Place of Publication United States ISSN/ISBN 1098-5336; 0099-2240 Accession Number PMID: 23892737 Language eng SubFile Journal Article; IM DOI 10.1128/AEM.01740-13 [doi] Output Language Unknown(0) PMID 23892737 Abstract CTX-M-producing Escherichia coli is the predominant type of extended-spectrum beta-lactamase (ESBL)-producing E. coli worldwide. In this study, molecular typing was conducted for 139 CTX-M-producing E. coli isolates, phenotypically positive for ESBLs, isolated from environmental water, swine, healthy humans, and hospitalized patients in Hangzhou, China. The antibiotic resistance profiles of the isolates for the cephalosporins and fluoroquinolones were determined. The isolates showed 100% resistance to cefotaxime and ceftriaxone while maintaining relatively high susceptibility to cefoxitin, cefepime, and ceftazidime. A total of 61.9% (86/139) of the isolates, regardless of origin, showed high resistance to fluoroquinolones. PCRs and DNA sequencing indicated that blaCTX-M-14 was the most prevalent CTX-M-9 group gene and that blaCTX-M-15 and blaCTX-M-55 were the dominant CTX-M-1 group genes. Isolates from all sources with CTX-M types belonging to the CTX-M-1 or CTX-M-9 group were most frequently associated with epidemics. Molecular homology analysis of the isolates, conducted by phylogenetic grouping, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST), demonstrated that the dominant clones belonged to B2-ST131, D-ST648, D-ST38, or A-CC10. These four sequence types (STs) were discovered in E. coli isolates both from humans and from environmental water, suggesting frequent and continuous intercompartment transmission between humans and the aquatic environment. Seven novel sequence types were identified in the current study. In conclusion, this study is the first to report the molecular homology analysis of CTX-M-producing E. coli isolates collected from water, swine, and healthy and hospitalized humans, suggesting that pathogens in the environment might originate both from humans and from animals. Descriptors Links Book Title Database Publisher Data Source Authors Hu,Y.Y., Cai,J.C., Zhou,H.W., Chi,D., Zhang,X.F., Chen,W.L., Zhang,R., Chen,G.X. Original/Translated Title URL Date of Electronic 20130726 PMCID PMC3811354 Editors
Molecular monitoring of bacterial community structure in long-aged nukadoko: pickling bed of fermented rice bran dominated by slow-growing lactobacilli	2007	Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. nakayama@agr.kyushu-u.ac.jp	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Journal of bioscience and bioengineering Periodical, Abbrev. J.Biosci.Bioeng. Pub Date Free Form Dec Volume 104 Issue 6 Start Page 481 Other Pages 489 Notes LR: 20151119; JID: 100888800; 2007/05/28 [received]; 2007/09/18 [accepted]; ppublish Place of Publication Japan ISSN/ISBN 1389-1723; 1347-4421 Accession Number PMID: 18215635 Language eng SubFile Journal Article; IM DOI 10.1263/jbb.104.481 [doi] Output Language Unknown(0) PMID 18215635 Abstract Nukadoko is the fermented rice bran bed traditionally used for pickling vegetables in Japan. Here, we investigate the bacterial community structure of nukadoko using several culture-independent methods. Denaturing gradient gel electrophoresis (DGGE) and sequence analysis of V2-V3 16S rRNA gene (16S rDNA) fragments amplified from a long-aged nukadoko bacterial community indicated seven predominant operational taxonomic units (OTUs) closely related to known Lactobacillus species. Phylogenetic analysis of these OTUs indicated a major cluster consisting of six OTUs including a dominant OTU closely related to Lactobacillus acidifarinae and one distinct OTU corresponding to Lactobacillus acetotolerans. L. acetotolerans was commonly detected as a dominant species in samples from different seasons. The succession of microbial community structure in the fermentation and ripening processes was investigated using a laboratory model nukadoko. The L. acidifarinae-like bacteria grew rapidly with a pH decrease in the first few days after inoculation, whereas L. acetotolerans grew slowly and became dominant after one week. Real-time quantitative polymerase chain reaction (Q-PCR) showed that the doubling time of L. acetotolerans was 12 h, while that of total bacteria was 4 h. Real-time quantitative reverse transcription polymerase chain reaction (Q-RT-PCR) targeting 16S rRNA showed a low metabolic activity of L. acetotolerans throughout the fermentation and ripening processes. Fluorescence in situ hybridization (FISH) showed that L. acetotolerans was a dominant bacterium in the ripening period and had a low metabolic activity. These results indicate that the slow-growing L. acetotolerans stably dominated nukadoko microbiota after the L. acidifarinae-like bacteria mainly contributed to the lactic acid fermentation of the rice bran. Descriptors Colony Count, Microbial/methods, Fermentation, Lactobacillus/classification/genetics/isolation & purification, Oryza/microbiology, Reverse Transcriptase Polymerase Chain Reaction/methods Links Book Title Database Publisher Data Source Authors Nakayama,J., Hoshiko,H., Fukuda,M., Tanaka,H., Sakamoto,N., Tanaka,S., Ohue,K., Sakai,K., Sonomoto,K. Original/Translated Title URL Date of Electronic PMCID Editors
Molecular identification of the microbiota of French sourdough using temporal temperature gradient gel electrophoresis	2007	Laboratoire de Microbiologie Alimentaire et Industrielle (LMAI), Unite de Recherche QM2A, ENITIAA, rue de la Geraudiere, BP 82225, 44322, Nantes Cedex 3, France. mounir.ferchichi@yahoo.fr	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Food Microbiology Periodical, Abbrev. Food Microbiol. Pub Date Free Form Oct-Dec Volume 24 Issue 8-Jul Start Page 678 Other Pages 686 Notes JID: 8601127; 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); 2007/01/18 [received]; 2007/04/17 [revised]; 2007/04/19 [accepted]; 2007/05/03 [aheadofprint]; ppublish Place of Publication England ISSN/ISBN 0740-0020; 0740-0020 Accession Number PMID: 17613364 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI S0740-0020(07)00057-3 [pii] Output Language Unknown(0) PMID 17613364 Abstract The microbiota of four industrial French sourdoughs (BF, GO, VB and RF) was characterized by PCR temporal temperature gel electrophoresis (TTGE). The TTGE technique reveals differences in the 16S rDNA V6-V8 regions of these bacteria. DNA was extracted directly from sourdough samples. A specific TTGE fingerprint was determined for 30 bacterial species, including members of the genera Lactobacillus, Leuconostoc and Weissella, all known to be present in sourdough. These sourdoughs contain different species of lactic acid bacteria (LAB) depending on ecological conditions prevailing in the different sourdough fermentations. Only a few LAB species were found to be competitive and became dominant. Lactobacillus sanfranciscensis was observed as the most frequently found species. In sourdough GO, L. sanfranciscensis, Lactobacillus panis and two new species, Lactobacillus nantensis and Lactobacillus hammesii, were detected. Sourdough BF contain L. sanfranciscensis, Lactobacillus spicheri and Lactobacillus pontis. In sourdough VB, which differed in the process temperature, we identified exclusively L. sanfranciscensis and Leuconostoc mesenteroides subsp. mesenteroides. Lactobacillus frumenti, L. hammesii and Lacobacillus paralimentarius became the predominant species in sourdough RF. Compared with conventional bacteriological methods, the use of this new molecular approach to analyze the sourdough ecosystem should therefore allow a more complete and rapid assessment of its specific microbiota. Descriptors Bread/microbiology, Colony Count, Microbial, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Electrophoresis, Agar Gel/methods, Fermentation, Food Microbiology, Lactobacillus/classification/isolation & purification, Leuconostoc/classification/isolation & purification, Phylogeny, Polymerase Chain Reaction/methods, RNA, Ribosomal, 16S/chemistry/genetics, Species Specificity, Temperature Links Book Title Database Publisher Data Source Authors Ferchichi,M., Valcheva,R., Prevost,H., Onno,B., Dousset,X. Original/Translated Title URL Date of Electronic 20070503 PMCID Editors
Molecular identification and quantification of lactic acid bacteria in traditional fermented dairy foods of Russia	2015	Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P. R. China, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, P. R. China.; Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Journal of dairy science Periodical, Abbrev. J.Dairy Sci. Pub Date Free Form Aug Volume 98 Issue 8 Start Page 5143 Other Pages 5154 Notes CI: Copyright (c) 2015; GENBANK/KC836519; GENBANK/KC836520; GENBANK/KC836521; GENBANK/KC836522; GENBANK/KC836523; GENBANK/KC836524; GENBANK/KC836525; GENBANK/KC836526; GENBANK/KC836527; GENBANK/KC836528; GENBANK/KC836529; GENBANK/KC836530; GENBANK/KC83653 Place of Publication United States ISSN/ISBN 1525-3198; 0022-0302 Accession Number PMID: 26004836 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.3168/jds.2015-9460 [doi] Output Language Unknown(0) PMID 26004836 Abstract Russian traditional fermented dairy foods have been consumed for thousands of years. However, little research has focused on exploiting lactic acid bacteria (LAB) resources and analyzing the LAB composition of Russian traditional fermented dairy foods. In the present study, we cultured LAB isolated from fermented mare and cow milks, sour cream, and cheese collected from Kalmykiya, Buryats, and Tuva regions of Russia. Seven lactobacillus species and the Bifidobacterium genus were quantified by quantitative PCR. The LAB counts in these samples ranged from 3.18 to 9.77 log cfu/mL (or per gram). In total, 599 LAB strains were obtained from these samples using de Man, Rogosa, and Sharpe agar and M17 agar. The identified LAB belonged to 7 genera and 30 species by 16S rRNA and murE gene sequencing and multiplex PCR assay. The predominant LAB isolates were Lactobacillus helveticus (176 strains) and Lactobacillus plantarum (63 strains), which represented 39.9% of all isolates. The quantitative PCR results revealed that counts of 7 lactobacilli species and Bifidobacterium spp. of 30 fermented cow milk samples ranged from 1.19+/-0.34 (Lactobacillus helveticus in Tuva) to 8.09+/-0.71 (Lactobacillus acidophilus in Kalmykiya) log cfu/mL of fermented cow milk (mean +/- standard error). The numbers of Bifidobacterium spp., Lb. plantarum, Lb. helveticus, and Lb. acidophilus revealed no significant difference between the 3 regions; nevertheless, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus sakei, and Lactobacillus delbrueckii ssp. bulgaricus exhibited different degrees of variation across 3 regions. The results demonstrate that traditional fermented dairy products from different regions of Russia have complex compositions of LAB species. The diversity of LAB might be related to the type of fermented dairy product, geographical origin, and manufacturing process. Descriptors Links Book Title Database Publisher American Dairy Science Association. Published by Elsevier Inc Data Source Authors Yu,J., Wang,H.M., Zha,M.S., Qing,Y.T., Bai,N., Ren,Y., Xi,X.X., Liu,W.J., Menghe,B.L., Zhang,H.P. Original/Translated Title URL Date of Electronic 20150523 PMCID Editors
Molecular epidemiology of extended-spectrum beta-lactamases and Escherichia coli isolated from retail foods including chicken meat in Japan	2014	1 Department of Pathophysiological Laboratory Science, Nagoya University Graduate School of Medicine , Nagoya, Japan .	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Foodborne pathogens and disease Periodical, Abbrev. Foodborne Pathog.Dis. Pub Date Free Form Feb Volume 11 Issue 2 Start Page 104 Other Pages 110 Notes JID: 101120121; 0 (DNA, Bacterial); 0 (Fluoroquinolones); EC 3.5.2.- (beta-lactamase CTX-2); EC 3.5.2.- (beta-lactamase TEM-3); EC 3.5.2.6 (beta-Lactamases); 2013/10/04 [aheadofprint]; ppublish Place of Publication United States ISSN/ISBN 1556-7125; 1535-3141 Accession Number PMID: 24093132 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1089/fpd.2013.1608 [doi] Output Language Unknown(0) PMID 24093132 Abstract Contamination of retail meat with extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli has been reported, but only limited data have been documented in Japan. One hundred fifty-three retail foods including chicken meat, beef, pork, and vegetables were purchased from 29 supermarkets between January and October in 2010. ESBL producers were recovered from each food sample using McConkey agar plate supplemented with 1 mg/L of cefotaxime. ESBL type was identified by DNA sequencing analysis after polymerase chain reaction amplification. Antibiogram, O serotype, plasmid replicon type, pulsotype, and multilocus sequence type were also determined. Fifty-two epidemiologically unrelated Escherichia coli isolates producing ESBL were recovered from 35 (22.9%) of 153 samples, all of which were chicken meat. ESBL types were mainly CTX-M-2 group followed by CTX-M-1 group and CTX-M-8 group. The numbers of bacterial isolates (8 of 21, 38.1%) harboring bla(CTX-M-8) recovered from imported meat samples were significantly larger than those of domestic ones (one of 31, 3.2%) (p Descriptors Links Book Title Database Publisher Data Source Authors Kawamura,K., Goto,K., Nakane,K., Arakawa,Y. Original/Translated Title URL Date of Electronic 20131004 PMCID Editors
Molecular epidemiology of extended-spectrum beta-lactamase-producing Escherichia coli in Tunisia and characterization of their virulence factors and plasmid addiction systems	2013	Laboratoire de Microbiologie, Hopital Habib Bourguiba, Sfax, Tunisie. basma_mnif@yahoo.fr	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full BMC microbiology Periodical, Abbrev. BMC Microbiol. Pub Date Free Form 25-Jun Volume 13 Issue Start Page 147 Other Pages 2180-13-147 Notes LR: 20150423; JID: 100966981; 0 (Virulence Factors); EC 3.5.2.6 (beta-Lactamases); OID: NLM: PMC3701463; 2013/03/28 [received]; 2013/05/27 [accepted]; 2013/06/25 [aheadofprint]; epublish Place of Publication England ISSN/ISBN 1471-2180; 1471-2180 Accession Number PMID: 23800277 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI 10.1186/1471-2180-13-147 [doi] Output Language Unknown(0) PMID 23800277 Abstract BACKGROUND: Extended-spectrum beta-lactamases (ESBLs), particularly CTX-M- type ESBLs, are among the most important resistance determinants spreading worldwide in Enterobacteriaceae. The aim of this study was to characterize a collection of 163 ESBL-producing Escherichia coli collected in Tunisia, their ESBL-encoding plasmids and plasmid associated addiction systems. RESULTS: The collection comprised 163 ESBL producers collected from two university hospitals of Sfax between 1989 and 2009. 118 isolates harbored blaCTX-M gene (101 blaCTX-M-15 gene and 17 blaCTX-M-14 gene). 49 isolates carried blaSHV-12 gene, 9 blaSHV-2a gene and only 3 blaTEM-26 gene. 16 isolates produced both CTX-M and SHV-12. The 101 CTX-M-15-producing isolates were significantly associated to phylogroup B2 and exhibiting a high number of virulence factors. 24 (23.7%) of the group B2 isolates belonged to clonal complex ST131. Pulsed-field gel electrophoresis (PFGE) typing revealed a genetic diversity of the isolates. 144 ESBL determinants were transferable mostly by conjugation. The majority of plasmid carrying blaCTX-M-15 genes (72/88) were assigned to various single replicon or multireplicon IncF types and had significantly a higher frequency of addiction systems, notably the VagCD module. CONCLUSION: This study demonstrates that the dissemination of CTX-M-15 producing E. coli in our setting was due to the spread of various IncF-type plasmids harboring multiple addiction systems, into related clones with high frequency of virulence determinants. Descriptors Links Book Title Database Publisher Data Source Authors Mnif,B., Harhour,H., Jdidi,J., Mahjoubi,F., Genel,N., Arlet,G., Hammami,A. Original/Translated Title URL Date of Electronic 20130625 PMCID PMC3701463 Editors
Molecular diversity of lactic acid bacteria from cassava sour starch (Colombia)	2000	Institut de Recherche pour le Developpement, Laboratoire de Biotechnologie Microbienne Tropicale, Montpellier, France.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Systematic and applied microbiology Periodical, Abbrev. Syst.Appl.Microbiol. Pub Date Free Form Jun Volume 23 Issue 2 Start Page 285 Other Pages 291 Notes LR: 20131121; GENBANK/AF000162; GENBANK/AF000163; JID: 8306133; 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); 33X04XA5AT (Lactic Acid); 9005-25-8 (Starch); ppublish Place of Publication GERMANY ISSN/ISBN 0723-2020; 0723-2020 Accession Number PMID: 10930082 Language eng SubFile Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; IM DOI S0723-2020(00)80016-8 [pii] Output Language Unknown(0) PMID 10930082 Abstract Lactic acid bacteria and more particularly lactobacilli and Leuconostoc, are widely found in a wide variety of traditional fermented foods of tropical countries, made with cereals, tubers, meat or fish. These products represent a source of bacterial diversity that cannot be accurately analysed using classical phenotypic and biochemical tests. In the present work, the identification and the molecular diversity of lactic acid bacteria isolated from cassava sour starch fermentation were assessed by using a combination of complementary molecular methods: Randomly Amplified Polymorphic DNA fingerprinting (RAPD), plasmid profiling, hybridization using rRNA phylogenetic probes and partial 16S rDNA sequencing. The results revealed a large diversity of bacterial species (Lb. manihotivorans, Lb. plantarum, Lb. casei, Lb. hilgardii, Lb. buchneri, Lb. fermentum, Ln. mesenteroides and Pediococcus sp.). However, the most frequently isolated species were Lb. plantarum and Lb. manihotivorans. The RAPD analysis revealed a large molecular diversity between Lb. manihotivorans or Lb. plantarum strains. These results, observed on a rather limited number of samples, reveal that significant bacterial diversity is generated in traditional cassava sour starch fermentations. We propose that the presence of the amylolytic Lb. manihotivorans strains could have a role in sour starch processing. Descriptors DNA, Ribosomal, Fermentation, Food Microbiology, Genetic Variation, Lactic Acid, Lactobacillus/classification/genetics, Manihot/microbiology, Molecular Sequence Data, Pediococcus/classification/genetics, Phylogeny, Plasmids/genetics, RNA, Ribosomal, 16S, Random Amplified Polymorphic DNA Technique, Starch Links Book Title Database Publisher Data Source Authors Omar,N. B., Ampe,F., Raimbault,M., Guyot,J. P., Tailliez,P. Original/Translated Title URL Date of Electronic PMCID Editors

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