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The subject of interest is:
waterpipe, water-pipe, nargile, narghile, arguileh, arguile, shisha, sheesha, chichi, hubble bubble, hubbly bubbly, goza, hookah, bong
| Title Sort ascending | Pub Year | Author | SearchLink |
|---|---|---|---|
| Physical activity, obesity and gastroesophageal reflux disease in the general population | 2012 | Unit of Upper Gastrointestinal Research, Department of Molecular Medicine and Surgery, Karolinska Institutet, SE-17176 Stockholm, Sweden. therese.djarv@ki.se | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
World journal of gastroenterology
Periodical, Abbrev.
World J.Gastroenterol.
Pub Date Free Form
28-Jul
Volume
18
Issue
28
Start Page
3710
Other Pages
3714
Notes
LR: 20151119; JID: 100883448; OID: NLM: PMC3406423; OTO: NOTNLM; 2012/02/07 [received]; 2012/05/11 [revised]; 2012/05/26 [accepted]; ppublish
Place of Publication
China
ISSN/ISBN
2219-2840; 1007-9327
Accession Number
PMID: 22851863
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.3748/wjg.v18.i28.3710 [doi]
Output Language
Unknown(0)
PMID
22851863
Abstract
AIM: To clarify the association between physical activity and gastroesophageal reflux disease (GERD) in non-obese and obese people. METHODS: A Swedish population-based cross-sectional survey was conducted. Participants aged 40-79 years were randomly selected from the Swedish Registry of the Total Population. Data on physical activity, GERD, body mass index (BMI) and the covariates age, gender, comorbidity, education, sleeping problems, and tobacco smoking were obtained using validated questionnaires. GERD was self-reported and defined as heartburn or regurgitation at least once weekly, and having at least moderate problems from such symptoms. Frequency of physical activity was categorized into three groups: (1) "high" (several times/week); (2) "intermediate" (approximately once weekly); and (3) "low" (1-3 times/mo or less). Analyses were stratified for participants with "normal weight" (BMI 30 kg/m(2)). Multivariate logistic regression was used to calculate odds ratios (ORs) with 95% confidence intervals (CIs), adjusted for potential confounding by covariates. RESULTS: Of 6969 eligible and randomly selected individuals, 4910 (70.5%) participated. High frequency of physical activity was reported by 2463 (50%) participants, GERD was identified in 472 (10%) participants, and obesity was found in 680 (14%). There were 226 (5%) individuals with missing information about BMI. Normal weight, overweight and obese participants were similar regarding distribution of gender and tobacco smoking status, while obese participants were on average slightly older, had fewer years of education, more comorbidity, slightly more sleeping problems, lower frequency of physical activity, and higher occurrence of GERD. Among the 2146 normal-weight participants, crude point estimates indicated a decreased risk of GERD among individuals with high frequency of physical activity (OR: 0.59, 95% CI: 0.39-0.89), compared to low frequency of physical activity. However, after adjustment for potential confounding factors, neither intermediate (OR: 1.30, 95% CI: 0.75-2.26) nor high (OR: 0.99, 95% CI: 0.62-1.60) frequency of physical activity was followed by decreased risk of GERD. Sleeping problems and high comorbidity were identified as potential confounders. Among the 1859 overweight participants, crude point estimates indicated no increased or decreased risk of GERD among individuals with intermediate or high frequency of physical activity, compared to low frequency. After adjustment for confounding, neither intermediate (OR: 0.75, 95% CI: 0.46-1.22) nor high frequency of physical activity were followed by increased or decreased risk of GERD compared to low frequency among nonobese participants. Sleeping problems and high comorbidity were identified as potential confounders for overweight participants. In obese individuals, crude ORs were similar to the adjusted ORs and no particular confounding factors were identified. Intermediate frequency of physical activity was associated with a decreased occurrence of GERD compared to low frequency of physical activity (adjusted OR: 0.41, 95% CI: 0.22-0.77). CONCLUSION: Intermediate frequency of physical activity might decrease the risk of GERD among obese individuals, while no influence of physical activity on GERD was found in non-obese people.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Djarv,T., Wikman,A., Nordenstedt,H., Johar,A., Lagergren,J., Lagergren,P.
Original/Translated Title
URL
Date of Electronic
PMCID
PMC3406423
Editors
|
|||
| Physical Activity and Snus: Is There a Link? | 2015 | Department for Ambulatory Care and Community Medicine, University of Lausanne, Rue du Bugnon 44, 1011 Lausanne, Switzerland. steph.henninger@gmail.com.; Department for Ambulatory Care and Community Medicine, University of Lausanne, Rue du Bugnon 44, 1011 | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International journal of environmental research and public health
Periodical, Abbrev.
Int.J.Environ.Res.Public.Health.
Pub Date Free Form
25-Jun
Volume
12
Issue
7
Start Page
7185
Other Pages
7198
Notes
LR: 20150730; JID: 101238455; OID: NLM: PMC4515649; OTO: NOTNLM; 2015/03/11 [received]; 2015/05/30 [revised]; 2015/06/15 [accepted]; epublish
Place of Publication
Switzerland
ISSN/ISBN
1660-4601; 1660-4601
Accession Number
PMID: 26121189
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.3390/ijerph120707185 [doi]
Output Language
Unknown(0)
PMID
26121189
Abstract
The study aimed at assessing the link between physical activity (PA), sports activity and snus use among young men in Switzerland. Data from the Cohort Study on Substance Use Risk Factors (C-SURF) were used to measure PA with the International Physical Activity Questionnaire and sports activity with a single item. Multivariate logistic regression analysis was conducted to measure the association between snus use, PA and sports activity. Similar models were run for smoking and snuff use. Snus use increased in a dose-response association with PA (high level: OR = 1.72; 95% CI 1.16-2.55) and with individuals exercising once a week or more often (OR = 1.65; 95% CI 1.26-2.16; p
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Henninger,S., Fischer,R., Cornuz,J., Studer,J., Gmel,G.
Original/Translated Title
URL
Date of Electronic
20150625
PMCID
PMC4515649
Editors
|
|||
| Phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16S ribosomal DNA analysis | 2003 | National Agricultural Research Organization, National Institute of Livestock and Grassland Science, Nishinasuno-machi, Tochigi-ken 329-2793, Japan. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Applied and Environmental Microbiology
Periodical, Abbrev.
Appl.Environ.Microbiol.
Pub Date Free Form
Jan
Volume
69
Issue
1
Start Page
444
Other Pages
451
Notes
LR: 20151119; GENBANK/AF515219; GENBANK/AF515220; GENBANK/AF515221; GENBANK/AF515222; GENBANK/AF515223; GENBANK/AF515224; GENBANK/AF515225; GENBANK/AF515226; GENBANK/AF515227; GENBANK/AF515228; GENBANK/AF515229; JID: 7605801; 0 (DNA, Ribosomal); 0 (RNA, R
Place of Publication
United States
ISSN/ISBN
0099-2240; 0099-2240
Accession Number
PMID: 12514026
Language
eng
SubFile
Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
12514026
Abstract
A total of 161 low-G+C-content gram-positive bacteria isolated from whole-crop paddy rice silage were classified and subjected to phenotypic and genetic analyses. Based on morphological and biochemical characters, these presumptive lactic acid bacterium (LAB) isolates were divided into 10 groups that included members of the genera Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, and WEISSELLA: Analysis of the 16S ribosomal DNA (rDNA) was used to confirm the presence of the predominant groups indicated by phenotypic analysis and to determine the phylogenetic affiliation of representative strains. The virtually complete 16S rRNA gene was PCR amplified and sequenced. The sequences from the various LAB isolates showed high degrees of similarity to those of the GenBank reference strains (between 98.7 and 99.8%). Phylogenetic trees based on the 16S rDNA sequence displayed high consistency, with nodes supported by high bootstrap values. With the exception of one species, the genetic data was in agreement with the phenotypic identification. The prevalent LAB, predominantly homofermentative (66%), consisted of Lactobacillus plantarum (24%), Lactococcus lactis (22%), Leuconostoc pseudomesenteroides (20%), Pediococcus acidilactici (11%), Lactobacillus brevis (11%), Enterococcus faecalis (7%), Weissella kimchii (3%), and Pediococcus pentosaceus (2%). The present study, the first to fully document rice-associated LAB, showed a very diverse community of LAB with a relatively high number of species involved in the fermentation process of paddy rice silage. The comprehensive 16S rDNA-based approach to describing LAB community structure was valuable in revealing the large diversity of bacteria inhabiting paddy rice silage and enabling the future design of appropriate inoculants aimed at improving its fermentation quality.
Descriptors
DNA, Ribosomal/analysis, Fermentation, Genetic Variation, Lactobacillus/classification/genetics/isolation & purification/metabolism, Molecular Sequence Data, Oryza/microbiology, Phenotype, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Silage/microbiology, Streptococcaceae/classification/genetics/isolation & purification/metabolism
Links
Book Title
Database
Publisher
Data Source
Authors
Ennahar,S., Cai,Y., Fujita,Y.
Original/Translated Title
URL
Date of Electronic
PMCID
PMC152408
Editors
|
|||
| Phylogenetic analysis of a biofilm bacterial population in a water pipeline in the Gulf of Mexico | 2006 | Hernández-RodrÃguez, C., Departamento de MicrobiologÃa, Escuela Nacional de Ciencias Biológicas, IPN, 06400 Mexico, D.F., Mexico | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
FEMS microbiology ecology
Periodical, Abbrev.
FEMS Microbiol.Ecol.
Pub Date Free Form
/
Volume
58
Issue
1
Start Page
145
Other Pages
154
Notes
Place of Publication
ISSN/ISBN
0168-6496; 1574-6941
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
The aim of this study was to assess the bacterial diversity associated with a corrosive biofilm in a steel pipeline from the Gulf of Mexico used to inject marine water into the oil reservoir. Several aerobic and heterotrophic bacteria were isolated and identified by 16S rRNA gene sequence analysis. Metagenomic DNA was also extracted to perform a denaturing gradient gel electrophoresis analysis of ribosomal genes and to construct a 16S rRNA gene metagenomic library. Denaturing gradient gel electrophoresis profiles and ribosomal libraries exhibited a limited bacterial diversity. Most of the species detected in the ribosomal library or isolated from the pipeline were assigned to Proteobacteria (Halomonas spp., Idiomarina spp., Marinobacter aquaeolei, Thalassospira sp., Silicibacter sp. and Chromohalobacter sp.) and Bacilli (Bacillus spp. and Exiguobacterium spp.). This is the first report that associates some of these bacteria with a corrosive biofilm. It is relevant that no sulfate-reducing bacteria were isolated or detected by a PCR-based method. The diversity and relative abundance of bacteria from water pipeline biofilms may contribute to an understanding of the complexity and mechanisms of metal corrosion during marine water injection in oil secondary recovery. © 2006 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved.
Descriptors
oil, RNA 16S, steel, aerobic bacterium, article, Bacilli, Bacillus, bacterial strain, bacterium identification, bacterium isolation, biofilm, Chromohalobacter, controlled study, corrosion, Exiguobacterium, gel electrophoresis, gene library, gene sequence, Halomonas, heterotrophy, marine environment, Marinobacter hydrocarbonoclasticus, Mexico, microbial diversity, nonhuman, nucleotide sequence, phylogeny, pipeline, polymerase chain reaction, priority journal, Proteobacteria, reservoir, ribosome, Silicibacter, Thalassospira, water analysis
Links
Book Title
Database
Embase; MEDLINE
Publisher
Data Source
Embase
Authors
López,M. A., Javier Zavala DÃaz De La Serna,F., Jan-Roblero,J., Romero,J. M., Hernández-RodrÃguez,C.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
|
|||
| Phenyl-functionalization of titanium dioxide-nanosheets coating fabricated on a titanium wire for selective solid-phase microextraction of polycyclic aromatic hydrocarbons from environment water samples | 2015 | College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.; College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.; College of Chemistry and Chemical Engineering, Northwes | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Talanta
Periodical, Abbrev.
Talanta
Pub Date Free Form
1-Nov
Volume
144
Issue
Start Page
998
Other Pages
1006
Notes
CI: Copyright (c) 2015; JID: 2984816R; 0 (Polycyclic Hydrocarbons, Aromatic); 0 (Thioglycolates); 0 (Waste Water); 0 (Water Pollutants, Chemical); 15FIX9V2JP (titanium dioxide); 7857H94KHM (2-mercaptoacetate); D1JT611TNE (Titanium); OTO: NOTNLM; 2015/04/2
Place of Publication
Netherlands
ISSN/ISBN
1873-3573; 0039-9140
Accession Number
PMID: 26452919
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.talanta.2015.07.064 [doi]
Output Language
Unknown(0)
PMID
26452919
Abstract
A novel titanium dioxide-nanosheets coating on a titanium wire (TiO2NS-Ti) was in situ fabricated by one-step electrochemical anodization in ethylene glycol with ammonium fluoride and followed by phenyl-functionalization for selective solid-phase microextraction (SPME). The fabricated TiO2NS coating exhibits higher specific surface area and more active sites, it also provides an ideal nanostructure and a robust substrate for subsequent surface modification. These characteristics were useful for efficient extraction. The SPME performance of phenyl-functionalized TiO2NS-Ti (ph-TiO2NS-Ti) fiber was evaluated by using ultraviolet filters, polychlorinated biphenyls and polycyclic aromatic hydrocarbons (PAHs) as model compounds coupled to high performance liquid chromatography with UV detection (HPLC-UV). It was found that the ph-TiO2NS-Ti fiber exhibited high extraction capability, good selectivity and rapid mass transfer for PAHs. The main parameters affecting extraction performance were investigated and optimized. Under optimized conditions, the proposed fiber showed good extraction efficiency comparable to those of commercial polydimethylsiloxane and polyacrylate fibers toward PAHs. The calibration graphs were linear over the range of 0.05-300 microg L(-1). The limits of detection of the proposed method were 0.008-0.043 microg L(-1) (S/N=3). Single fiber repeatability varied from 3.51% to 5.23% and fiber-to-fiber reproducibility ranged from 4.43% to 7.65% for the extraction of water spiked with 25 microg L(-1) each analyte (n=5). The established SPME-HPLC-UV method was successfully applied to selective concentration and sensitive determination of target PAHs from real environmental water samples with recoveries from 86.2% to 112% at the spiking level of 10 microg L(-1) and 50 microg L(-1). The relative standard deviations were below 9.45%. Furthermore, the ph-TiO2NS-Ti fiber can be fabricated in a reproducible manner, and has high stability and long service lifetime.
Descriptors
Links
Book Title
Database
Publisher
Elsevier B.V
Data Source
Authors
Guo,M., Song,W., Wang,T., Li,Y., Wang,X., Du,X.
Original/Translated Title
URL
Date of Electronic
20150726
PMCID
Editors
|
|||
| Phenotypic and phylogenetic analysis of lactic acid bacteria isolated from forage crops and grasses in the Tibetan Plateau | 2012 | Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, 450052, Zhengzhou, Henan, P. R. China. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of microbiology (Seoul, Korea)
Periodical, Abbrev.
J.Microbiol.
Pub Date Free Form
Feb
Volume
50
Issue
1
Start Page
63
Other Pages
71
Notes
GENBANK/AB602799; GENBANK/AB602800; GENBANK/AB602801; GENBANK/AB602802; GENBANK/AB602803; GENBANK/AB602804; GENBANK/AB602805; GENBANK/AB602806; GENBANK/AB602807; GENBANK/AB602808; GENBANK/AB602809; GENBANK/AB602810; GENBANK/AB602811; GENBANK/AB602812; GEN
Place of Publication
Korea (South)
ISSN/ISBN
1976-3794; 1225-8873
Accession Number
PMID: 22367939
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1007/s12275-012-1284-5 [doi]
Output Language
Unknown(0)
PMID
22367939
Abstract
A total of 140 lactic acid bacteria (LAB) strains were isolated from corn, alfalfa, clover, sainfoin, and Indian goosegrass in the Tibetan Plateau. According to phenotypic and chemotaxonomic characteristics, 16S rDNA sequence, and recA gene PCR amplification, these LAB isolates were identified as belonging to five genera and nine species. Corn contained more LAB species than other forage crops. Leuconostoc pseudomesenteroides, Lactococcus lactis subsp. lactis, Lactobacillus brevis, and Weissella paramesenteroides were dominant members of the LAB population on alfalfa, clover, sainfoin, and Indian goosegrass, respectively. The comprehensive 16S rDNA and recA-based approach effectively described the LAB community structure of the relatively abundant LAB species distributed on different forage crops. This is the first report describing the diversity and natural populations of LAB associated with Tibetan forage crops, and most isolates grow well at or below 10 degrees C. The results will be valuable for the future design of appropriate inoculants for silage fermentation in this very cold area.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Pang,H., Tan,Z., Qin,G., Wang,Y., Li,Z., Jin,Q., Cai,Y.
Original/Translated Title
URL
Date of Electronic
20120227
PMCID
Editors
|
|||
| Phenotypic and genotypic identification of lactic acid bacteria isolated from traditional pickles of the Cubuk region in Turkey | 2015 | Faculty of Engineering, Department of Food Engineering, Ankara University, Ankara, Turkey, sbagder@eng.ankara.edu.tr. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Folia microbiologica
Periodical, Abbrev.
Folia Microbiol.(Praha)
Pub Date Free Form
May
Volume
60
Issue
3
Start Page
241
Other Pages
251
Notes
JID: 0376757; 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 33X04XA5AT (Lactic Acid); 2014/07/03 [received]; 2014/11/03 [accepted]; 2014/11/18 [aheadofprint]; ppublish
Place of Publication
United States
ISSN/ISBN
1874-9356; 0015-5632
Accession Number
PMID: 25404550
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1007/s12223-014-0363-x [doi]
Output Language
Unknown(0)
PMID
25404550
Abstract
A total of 152 lactic acid bacteria (LAB) were isolated from pickles produced in the Ankara-Cubuk region. These isolates were clustered into eight groups on the basis of their phenotypic characteristics including cell morphology, CO2 production from glucose, growth at 10 and 45 degrees C, growth in 6.5 % NaCl, and growth at pH 9.6. API 50 CH carbohydrate fermentation test, 16S ribosomal RNA (rRNA) sequence analysis, and sodium dodecyl sulfate-acrylamide gel electrophoresis (SDS-PAGE) whole-cell protein profile analysis were also performed for precise identification of the isolates at the species level. Molecular identification revealed that the most prevalent LAB species involved in pickle fermentation were Pediococcus ethanolidurans (46 isolates, 30.3 %), Lactobacillus brevis (37 isolates, 24.3 %), Lactobacillus plantarum (37 isolates, 24.3 %), and Lactobacillus buchneri (15 isolates, 9.9 %). Other LAB were found in minor frequencies such as Pediococcus parvulus (8 isolates, 5.3 %), Lactobacillus namurensis (6 isolates, 3.9 %), Lactobacillus diolivorans (1 isolate, 0.7 %), Lactobacillus parabrevis (1 isolate, 0.7 %), and Enterococcus casseliflavus (1 isolate, 0.7 %). When results of phenotypic and genotypic identification methods were compared, differences in the species distribution of LAB associated with pickles were defined between the API and the 16S rRNA sequencing. The API 50 CHL test coincided with the 16S rRNA results in 71 out of the 152 tested isolates, indicating that API gave unreliable identification results. A clear correlation could not be found between the results of whole-cell SDS profiles and 16S rRNA sequencing. Therefore, molecular characterization by 16S rRNA sequencing was considered to be the most reliable method for identifying isolates. The results presented in this work provide insight in to the LAB population associated with traditional Cubuk pickles and constitute a LAB strain resource for further studies involving the development of starter cultures.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Bagder Elmaci,S., Tokatli,M., Dursun,D., Ozcelik,F., Sanlibaba,P.
Original/Translated Title
URL
Date of Electronic
20141118
PMCID
Editors
|
|||
| Phenotypic and genotypic identification of lactic acid bacteria isolated from ethnic fermented bamboo tender shoots of North East India | 2008 | Food Microbiology Laboratory, Department of Botany, Sikkim Government College, Gangtok 737102, Sikkim, India. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International journal of food microbiology
Periodical, Abbrev.
Int.J.Food Microbiol.
Pub Date Free Form
15-Jan
Volume
121
Issue
1
Start Page
35
Other Pages
40
Notes
JID: 8412849; 0 (DNA, Bacterial); 2007/04/05 [received]; 2007/08/29 [revised]; 2007/10/24 [accepted]; 2007/11/01 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
0168-1605; 0168-1605
Accession Number
PMID: 18036695
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
S0168-1605(07)00563-6 [pii]
Output Language
Unknown(0)
PMID
18036695
Abstract
Mesu, soidon, soibum and soijim are ethnic fermented bamboo tender shoot products prepared by the people in North East India. Microbiological analysis of mesu, soidon, soibum and soijim showed the population dominated by lactic acid bacteria (LAB) ranging up to 10(8) cfu g(-1). The phenotypic characterisation of predominant LAB isolated from the fermented bamboo shoot products was based on general morphology, physiological tests, API and Biolog systems. The genotypic characterisation of LAB was based on RAPD-PCR, rep PCR, species-specific PCR techniques, 16S rRNA gene sequencing and DNA-DNA hybridisation. Predominant functional LAB strains associated with the fermented bamboo shoot products were identified as Lactobacillus brevis, Lb. plantarum, Lb. curvatus, Pediococcus pentosaceus, Leuconostoc mesenteroides subsp. mesenteroides, Leuc. fallax, Leuc. lactis, Leuc. citreum and Enterococcus durans.
Descriptors
Bambusa/microbiology, Colony Count, Microbial, DNA, Bacterial/analysis, Enterococcus/classification/isolation & purification, Fermentation, Food Microbiology, Genotype, Humans, Hydrogen-Ion Concentration, India, Lactobacillus/classification/isolation & purification, Leuconostoc/classification/isolation & purification, Pediococcus/classification/isolation & purification, Phenotype, Phylogeny, Poaceae/microbiology, Random Amplified Polymorphic DNA Technique, Species Specificity
Links
Book Title
Database
Publisher
Data Source
Authors
Tamang,B., Tamang,J. P., Schillinger,U., Franz,C. M., Gores,M., Holzapfel,W. H.
Original/Translated Title
URL
Date of Electronic
20071101
PMCID
Editors
|
|||
| Phenotypic and genotypic characterization of lactic acid bacteria isolated from Artisanal Italian goat cheese | 2010 | Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche sez, Microbiologia Agraria Alimentare Ecologica, Universita degli Studi di Milano, Via Celoria, 2 201333 Milano, Italy. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of food protection
Periodical, Abbrev.
J.Food Prot.
Pub Date Free Form
Apr
Volume
73
Issue
4
Start Page
657
Other Pages
662
Notes
JID: 7703944; 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); ppublish
Place of Publication
United States
ISSN/ISBN
0362-028X; 0362-028X
Accession Number
PMID: 20377953
Language
eng
SubFile
Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
20377953
Abstract
The lactic acid bacteria community in traditional goat cheese produced in three dairies in Valsesia (Piemonte, Italy) was studied at different steps of the manufacturing process. These cheeses were produced from raw milk without starter bacteria, and no protocol was followed during the manufacturing process. Three hundred thirty-two isolates were characterized and grouped by results of both morphophysiological tests and random amplification of polymorphic DNA plus PCR analysis. Bacteria were identified by partial sequencing of the 16S rRNA gene. Lactococci were the dominant lactic acid bacteria in raw milk. Their initial numbers ranged from 5 to 7 log CFU ml(-1). Their levels increased during manufacturing and decreased during ripening. The growth trend for enterococci was comparable to that of lactococci, although enterococci counts were lower. Lactococcus lactis subsp. cremoris, Lactococcus garviae, and Enterococcus faecalis were the most frequently isolated species during goat cheese manufacturing, whereas the highest numbers of Enterococcus (E. faecium, E. durans, E. gilvus, and E. casseliflavus) were isolated with the greatest frequency from ripened cheese samples. Occasionally, Leuconostoc mesenteroides, Leuconostoc lactis, and Lactobacillus paraplantarum also were isolated.
Descriptors
Animals, Cheese/microbiology/standards, Colony Count, Microbial, DNA, Bacterial/analysis, Enterococcus/classification/growth & development/isolation & purification, Food Microbiology, Genotype, Goats, Humans, Industrial Microbiology, Italy, Lactobacillaceae/classification/growth & development/isolation & purification, Lactobacillus/classification/growth & development/isolation & purification, Lactococcus/classification/growth & development/isolation & purification, Leuconostoc/classification/growth & development/isolation & purification, Phenotype, Phylogeny, RNA, Ribosomal, 16S/analysis, Random Amplified Polymorphic DNA Technique, Species Specificity
Links
Book Title
Database
Publisher
Data Source
Authors
Colombo,E., Franzetti,L., Frusca,M., Scarpellini,M.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
|
|||
| Phenotypic and genotypic analyses of lactic acid bacteria in local fermented food, breast milk and faeces of mothers and their babies | 2011 | Lehrstuhl fur Technische Mikrobiologie, Technische Universitat Munchen, Weihenstephaner Steig 16, 85350 Freising, Germany. | Read more... |
|
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Systematic and applied microbiology
Periodical, Abbrev.
Syst.Appl.Microbiol.
Pub Date Free Form
Apr
Volume
34
Issue
2
Start Page
148
Other Pages
155
Notes
CI: Copyright (c) 2011; JID: 8306133; 2010/11/05 [received]; 2010/12/20 [revised]; 2010/12/21 [accepted]; ppublish
Place of Publication
Germany
ISSN/ISBN
1618-0984; 0723-2020
Accession Number
PMID: 21300508
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.syapm.2010.12.001 [doi]
Output Language
Unknown(0)
PMID
21300508
Abstract
Lactic acid bacteria (LAB) are generally accepted as beneficial to the host and their presence is directly influenced by ingestion of fermented food or probiotics. While the intestinal lactic microbiota is well-described knowledge on its routes of inoculation and competitiveness towards selective pressure shaping the intestinal microbiota is limited. In this study, LAB were isolated from faecal samples of breast feeding mothers living in Syria, from faeces of their infants, from breast milk as well as from fermented food, typically consumed in Syria. A total of 700 isolates were characterized by genetic fingerprinting with random amplified polymorphic DNA (RAPD) and identified by comparative 16S rDNA sequencing and Matrix Assisted Laser Desorption Ionization-Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS) analyses. Thirty six different species of Lactobacillus, Enterococcus, Streptococcus, Weissella and Pediococcus were identified. RAPD and MALDI-TOF-MS patterns allowed comparison of the lactic microbiota on species and strain level. Whereas some species were unique for one source, Lactobacillus plantarum, Lactobacillus fermentum, Pediococcus pentosaceus and Lactobacillus brevis were found in all sources. Interestingly, identical RAPD genotypes of L. plantarum, L. fermentum, L. brevis, Enterococcus faecium, Enterococcus faecalis and P. pentosaceus were found in the faeces of mothers, her milk and in faeces of her babies. Diversity of RAPD types found in food versus human samples suggests the importance of host factors in colonization and individual host specificity, and support the hypothesis that there is a vertical transfer of intestinal LAB from the mother's gut to her milk and through the milk to the infant's gut.
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Elsevier GmbH
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Albesharat,R., Ehrmann,M.A., Korakli,M., Yazaji,S., Vogel,R.F.
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