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Activity of caspofungin and voriconazole against clinical isolates of Candida and other medically important yeasts by the CLSI M-44A disk diffusion method with Neo-Sensitabs tablets 2008 Departamento de Microbiologia, ACIA, Barcelona, Spain. acarrillo@ya.com
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Chemotherapy
Periodical, Abbrev.
Chemotherapy
Pub Date Free Form
Volume
54
Issue
1
Start Page
38
Other Pages
42
Notes
LR: 20141120; CI: (c) 2007; JID: 0144731; 0 (Antifungal Agents); 0 (Echinocandins); 0 (Pyrimidines); 0 (Triazoles); 304NUG5GF4 (Itraconazole); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); F0XDI6ZL63 (caspofungin); JFU09I87TR (Voriconazole); R940
Place of Publication
Switzerland
ISSN/ISBN
1421-9794; 0009-3157
Accession Number
PMID: 18073469
Language
eng
SubFile
Comparative Study; Journal Article; IM
DOI
000112414 [pii]
Output Language
Unknown(0)
PMID
18073469
Abstract
In vitro activity of caspofungin and voriconazole against 184 clinical isolates of Candida and other medically important yeasts in comparison with that of fluconazole, ketoconazole, itraconazole and amphotericin B was determined by using a disk diffusion method (Neo-Sensitabs) standardized according to the recommendations of the CLSI documents M44-A and M44-S1 (same medium: Mueller-Hinton plus methylene blue; inoculum and minimal inhibitory concentration/zone breakpoints). Seventy-two percent of clinical isolates were susceptible to caspofungin, 23.6% showed an intermediate susceptibility (most of them were Candida parapsilosis) and 4.3% were resistant (values for Candida spp. were 71.2, 23.8 and 5%, respectively). For voriconazole, 96.7% of clinical isolates were susceptible and 3.3% were resistant (Candida spp.: 96 and 3.8%, respectively). Both caspofungin and voriconazole showed high activity against a wide variety of clinically important yeasts.
Descriptors
Amphotericin B/pharmacology, Antifungal Agents/pharmacology, Candida/drug effects, Cryptococcus/drug effects, Disk Diffusion Antimicrobial Tests, Drug Resistance, Fungal, Echinocandins/pharmacology, Fluconazole/pharmacology, Itraconazole/pharmacology, Ketoconazole/pharmacology, Pyrimidines/pharmacology, Rhodotorula/drug effects, Triazoles/pharmacology, Trichosporon/drug effects, Voriconazole
Links
Book Title
Database
Publisher
S. Karger AG, Basel
Data Source
Authors
Carrillo-Munoz,A. J., Quindos,G., del Valle,O., Santos,P., Giusiano,G., Ezkurra,P. A., Estivill,M. D., Casals,J. B.
Original/Translated Title
URL
Date of Electronic
20071210
PMCID
Editors
Shear bond strength of composite bonded to erbium:yttrium-aluminum-garnet laser-prepared dentin 2009 Department of Restorative Dentistry, School of Dentistry, Hacettepe University, Sihhiye, 06100, Ankara, Turkey. sgurgan@hacettepe.edu.tr
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Lasers in medical science
Periodical, Abbrev.
Lasers Med.Sci.
Pub Date Free Form
Jan
Volume
24
Issue
1
Start Page
117
Other Pages
122
Notes
LR: 20141120; JID: 8611515; 0 (Acrylic Resins); 0 (Adhesives); 0 (Composite Dental Resin); 0 (Composite Resins); 0 (Polyurethanes); 2007/06/08 [received]; 2007/10/22 [accepted]; 2007/12/12 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
0268-8921; 0268-8921
Accession Number
PMID: 18074164
Language
eng
SubFile
Journal Article; IM
DOI
10.1007/s10103-007-0532-0 [doi]
Output Language
Unknown(0)
PMID
18074164
Abstract
The purpose of this study was to evaluate the dentin bond strength to resin composite following erbium:yttrium-aluminum-garnet (Er:YAG) laser preparation using different adhesive systems. Seventy dentin specimens prepared from human molar teeth were randomly assigned to seven groups of ten. The first five groups were prepared with an Er:YAG laser 2940 nm at the manufacturer's recommended settings and (1) acid etched, and etch-and-rinse adhesive Excite was applied; (2) Excite was applied; (3) two-step self-etching adhesive AdheSE was applied; (4) laser etched (120 mJ/10 Hz), and Excite was applied; (5) laser etched, and AdheSE was applied. The last two groups were added as controls (prepared with a diamond bur): (6) acid etched, and Excite was applied; (7) AdheSE was applied. Nanohybrid composite cylinders 4 mm x 2 mm were bonded to the dentin surfaces. After the specimens had been stored in distilled water and had undergone thermocycling, the shear bond strength was tested and the data were analyzed statistically. The Duncan multiple comparison test showed that specimens prepared with a diamond bur and with acid and Excite applied showed the highest mean bond strength (13.01 +/- 2.09 MPa), followed by those prepared with Er:YAG and with AdheSE applied (11.5 +/- 3.59 MPa) and those prepared with a diamond bur and with AdheSE applied (10.75 +/- 1.95 MPa), but there were no significant differences among them (P > 0.05). Er:YAG-prepared specimens, with acid, Excite (3.28 +/- 0.95 MPa) and specimens that were laser etched and with AdheSE applied (3.37 +/- 0.63 MPa) showed the lowest mean values for bond strength (P < 0.05). The results suggested that dentin surfaces prepared with Er:YAG laser may provide comparable composite resin bond strengths depending on the adhesives used.
Descriptors
Acrylic Resins, Adhesives, Composite Resins, Dental Bonding, Dentin/radiation effects, Humans, In Vitro Techniques, Lasers, Solid-State, Molar, Polyurethanes
Links
Book Title
Database
Publisher
Data Source
Authors
Gurgan,S., Kiremitci,A., Cakir,F. Y., Yazici,E., Gorucu,J., Gutknecht,N.
Original/Translated Title
URL
Date of Electronic
20071212
PMCID
Editors
Bacterial biodiversity of traditional Zabady fermented milk 2008 Department of Dairy Science and Technology, Faculty of Agriculture, Alexandria University, Aflaton street, El-Shatby, Alexandria, Egypt. elbaradeig@yahoo.fr
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International journal of food microbiology
Periodical, Abbrev.
Int.J.Food Microbiol.
Pub Date Free Form
10-Feb
Volume
121
Issue
3
Start Page
295
Other Pages
301
Notes
JID: 8412849; 0 (DNA, Bacterial); 2006/11/09 [received]; 2007/04/11 [revised]; 2007/11/06 [accepted]; 2007/11/17 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
0168-1605; 0168-1605
Accession Number
PMID: 18077039
Language
eng
SubFile
Journal Article; IM
DOI
S0168-1605(07)00587-9 [pii]
Output Language
Unknown(0)
PMID
18077039
Abstract
The aim of this work was to identify the bacterial biodiversity of traditional Zabady fermented milk using PCR-temporal temperature gel electrophoresis (PCR-TTGE) and PCR-denaturing gradient gel electrophoresis (PCR-DGGE). Most of the identified bacterial species in Zabady samples belonged to lactic acid bacteria (LAB), e.g., Streptococcus thermophilus, Lactococcus garvieae, Lactococcus raffinolactis, Lactococcus lactis, Leuconostoc citreum, Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus johnsonii. Using the culture-dependent and independent methods, the streptococcal and lactococcal groups appeared to be the major bacterial species in Zabady fermented milk, whereas the lactobacilli were the minor ones. The main dominant species was St. thermophilus followed by Lc. garvieae. Other molecular tools, e.g., species-specific PCR assay and cloning and sequencing strategy were used to confirm the TTGE and DGGE results. Lc. garvieae, Lc. raffinolactis, Ln. citreum, and Lb. johnsonii were identified for the first time in this type of Egyptian fermented milk.
Descriptors
Biodiversity, Colony Count, Microbial, Cultured Milk Products/microbiology, DNA, Bacterial/analysis, Egypt, Electrophoresis, Gel, Pulsed-Field/methods, Food Microbiology, Lactobacillus/classification/isolation & purification, Lactococcus/classification/isolation & purification, Leuconostoc/classification/isolation & purification, Phylogeny, Polymerase Chain Reaction/methods, Species Specificity, Streptococcus/classification/isolation & purification
Links
Book Title
Database
Publisher
Data Source
Authors
El-Baradei,G., Delacroix-Buchet,A., Ogier,J. C.
Original/Translated Title
URL
Date of Electronic
20071117
PMCID
Editors
Study on the level of tobacco-generated smoke in several restautants and bars in Beijing, China 2007 Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi
Periodical, Abbrev.
Zhonghua Liu Xing Bing Xue Za Zhi
Pub Date Free Form
Aug
Volume
28
Issue
8
Start Page
738
Other Pages
741
Notes
JID: 8208604; 0 (Particulate Matter); 0 (Tobacco Smoke Pollution); ppublish
Place of Publication
China
ISSN/ISBN
0254-6450; 0254-6450
Accession Number
PMID: 18080555
Language
chi
SubFile
English Abstract; Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
18080555
Abstract
OBJECTIVE: To investigate the current smoking regulations and their impacts on the environmental tobacco smoke (ETS) levels inside restaurants and bars in Beijing. METHODS: Telephone survey was used to investigate the smoking regulations. TSI Sidepak AM510 was used to measure the level of fine particles less than 2.5 microns in diameter (PM2.5) in restaurants and bars. Analysis of variance and non-parametric rank tests were used to examine the association between indoor and outdoor PM2.5 levels and (1) smoking regulations; and (2) types of restaurants and bars. RESULTS: Of the 305 restaurants and bars surveyed, 27.9% had complete or partial smoking prohibiting rules. The average indoor PM2.5, level of the 92 restaurants and bars was 253.08 microg/m3 , 102.37% higher than the outdoor level. The average indoor and outdoor PM2.5 levels in the restaurants and bars with smoking ban regulations were 93.10 microg/m3 and 110.33 microg/m3 whole 289.34 microg/m3 and 128.40 microg/m3 in those without, respectively. The average indoor and outdoor PM2.5 levels of bars were 413.46 microg/m3 and 190.62 microg/m3, respectively, while in the western fast-food restaurants, they were 83.86 microg/m3 and 104.77 microg/m3, respectively. The outdoor PM2.5 levels were higher than the indoor levels in different classes of restaurants and bars. Furthermore, there was a significant positive correlation between PM2.5 levels and the number of smokers per cube meters (r = 0.47, P < 0.001). CONCLUSION: Smoking regulations could effectively reduce the ETS level in restaurants and bars.
Descriptors
Air Pollution, Indoor/analysis/legislation & jurisprudence, China, Environmental Monitoring, Particulate Matter/analysis, Restaurants, Smoking/legislation & jurisprudence, Tobacco Smoke Pollution/analysis/legislation & jurisprudence
Links
Book Title
Database
Publisher
Data Source
Authors
Kang,J. M., Jiang,Y., Lin,X. G., Yang,Y., Nan,Y., Li,Z., Liu,R. L., Feng,G. Z., Wei,X. S., Travers,M. J., Li,Q., Hyland,A.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Home exposures to environmental tobacco smoke and allergic symptoms among young children in Singapore 2008 Department of Building, School of Design and Environment, National University of Singapore, Singapore, Singapore. bdgzms@nus.edu.sg
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International archives of allergy and immunology
Periodical, Abbrev.
Int.Arch.Allergy Immunol.
Pub Date Free Form
Volume
146
Issue
1
Start Page
57
Other Pages
65
Notes
LR: 20151119; CI: Copyright 2007; JID: 9211652; 0 (Tobacco Smoke Pollution); 2007/04/11 [received]; 2007/09/05 [accepted]; 2007/12/14 [aheadofprint]; ppublish
Place of Publication
Switzerland
ISSN/ISBN
1423-0097; 1018-2438
Accession Number
PMID: 18087162
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
000112503 [pii]
Output Language
Unknown(0)
PMID
18087162
Abstract
BACKGROUND: Research relating environmental tobacco smoke (ETS) exposures have focused on childhood asthma. There have been fewer studies with conflicting results performed on associations of ETS exposures with allergic symptoms. We are interested to see if ETS exposures in the homes are associated with allergic symptoms among preschool children in Singapore where public smoking is banned. METHODS: A cross-sectional study adopting an expanded and modified ISAAC (International Study on Asthma and Allergies in Childhood) questionnaire for the evaluation of asthma and allergies was conducted on 6,794 children attending 120 randomly selected child care centers. Specific information on demographics and ETS exposures was obtained. Adjusted prevalence ratios (PRs) and 95% confidence intervals (CIs) were determined using Poisson multivariate regression with a log-link function and robust variance estimates as recommended for cross-sectional studies. RESULTS: The response proportion was 70.0%, and 4,759 children from 97 centers participated. After adjusting for covariates, it was found that home ETS exposure was associated with increased risks of current symptoms of rhinitis (PR 1.23; 95% CI 1.01-1.50) and rhinoconjunctivitis (PR 1.79; 95% CI 1.26-2.54). These associations followed dose-response trends with respect to number of cigarettes smoked or smokers in the homes. Home ETS exposures were also associated with higher PRs of wheeze, nocturnal cough and doctor-diagnosed asthma. Compared with paternal smoking, higher risks of the above outcomes were found for maternal smoking. CONCLUSION: Home ETS exposure is a risk factor associated with rhinitis and asthma among preschool children.
Descriptors
Child, Preschool, Cross-Sectional Studies, Environmental Exposure/adverse effects, Female, Humans, Hypersensitivity/epidemiology/immunology, Infant, Male, Prevalence, Singapore/epidemiology, Surveys and Questionnaires, Tobacco Smoke Pollution/adverse effects
Links
Book Title
Database
Publisher
S. Karger AG, Basel
Data Source
Authors
Zuraimi,M. S., Tham,K. W., Chew,F. T., Ooi,P. L., David,K.
Original/Translated Title
URL
Date of Electronic
20071214
PMCID
Editors
In vitro activity of amphotericin B and anidulafungin against Candida spp. biofilms 2007 Unidad de Microbiologia Experimental, Centro Investigacion, Hospital Universitario La Fe, Valencia, Spain.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Revista Iberoamericana De Micologia
Periodical, Abbrev.
Rev.Iberoam.Micol.
Pub Date Free Form
31-Dec
Volume
24
Issue
4
Start Page
272
Other Pages
277
Notes
LR: 20131121; JID: 9425531; 0 (Antifungal Agents); 0 (Echinocandins); 7XU7A7DROE (Amphotericin B); 9HLM53094I (anidulafungin); ppublish
Place of Publication
Spain
ISSN/ISBN
1130-1406; 1130-1406
Accession Number
PMID: 18095759
Language
spa
SubFile
Comparative Study; English Abstract; Journal Article; IM
DOI
200724272 [pii]
Output Language
Unknown(0)
PMID
18095759
Abstract
Invasive infections caused by Candida spp. are increasing worldwide and are becoming an important cause of morbidity and mortality in immunocompromised patients. A large number of manifestations of candidiasis are associated with the formation of biofilms on inert or biological surfaces. Candida spp. biofilms are recalcitrant to treatment with conventional antifungal therapies. The aim of this study was dual 1) to determine the prevalence of biofilm producers among clinical isolates from catheter (16 C. albicans ) and blood culture (2 C. albicans and 30 C. tropicalis), and 2) to determine the activity of amphotericin B and anidulafungin against C. albicans and C. tropicalis biofilms of 24 and 48 hours of maturation. Biofilms were developed using a 96-well microtitre plate model and production and activity of antifungal agents against biofilms were determined by the tetrazolium (XTT) reduction assay. Of catheter and blood isolates, 62.5 and 56.25%, respectively, produced biofilms. By species, 68.42% of C. albicans and 53.33% of C. tropicalis were biofilm producers. C. albicans biofilms showed more resistance to amphotericin B and anidulafungin than their planktonic counterparts. Complete killing of biofilms was never achieved, even at the highest concentrations of the drugs tested. Anidulafungin displayed more activity than amphotericin B against C. albicans biofilms of 24 hours of maturation (GM MIC 0.354 vs. 0.686 microg/ml), but against C. tropicalis biofilms amphotericin B was more active (GM MIC 11.285 vs. 0.476 microg/ml). In contrast, against biofilms with 48 hours maturation, amphotericin B was more active against both species.
Descriptors
Amphotericin B/pharmacology, Antifungal Agents/pharmacology, Biofilms/drug effects, Candida albicans/drug effects/physiology, Candida tropicalis/drug effects/physiology, Drug Evaluation, Preclinical, Drug Resistance, Fungal, Echinocandins/pharmacology, Microbial Sensitivity Tests, Species Specificity
Links
Book Title
Database
Publisher
Data Source
Authors
Valentin,A., Canton,E., Peman,J., Quindos,G.
Original/Translated Title
Actividad in vitro de la anfotericina B y la anidulafungina sobre biopeliculas de Candida albicans y Candida tropicalis
URL
Date of Electronic
PMCID
Editors
Characteristics associated with self-identification as a regular smoker and desire to quit among college students who smoke cigarettes 2008 Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024, USA.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco
Periodical, Abbrev.
Nicotine Tob.Res.
Pub Date Free Form
Jan
Volume
10
Issue
1
Start Page
69
Other Pages
76
Notes
GR: CA093967/CA/NCI NIH HHS/United States; JID: 9815751; ppublish
Place of Publication
England
ISSN/ISBN
1462-2203; 1462-2203
Accession Number
PMID: 18188747
Language
eng
SubFile
Journal Article; Research Support, N.I.H., Extramural; IM
DOI
10.1080/14622200701704202 [doi]
Output Language
Unknown(0)
PMID
18188747
Abstract
Tobacco use among college students increased substantially during the 1990s. Better understanding of college smokers is warranted to develop interventions specific to the needs of this population. We examined sociodemographic and tobacco-use characteristics associated with self-identification as a regular smoker and intentions to quit smoking among college students who smoke cigarettes. We conducted logistic regression analysis on baseline survey data from the Campus Health Action on Tobacco study, a 4-year group-randomized trial at 30 four-year colleges in Washington, Oregon, and Idaho. Students who self-identified as a regular smoker smoked more cigarettes before starting college, smoked a greater number of cigarettes the prior 30 days, smoked more cigarettes per day, and were more likely to smoke within 30 min of waking up, compared with students who were current smokers but did not consider themselves regular smokers. Females, older students, and those who had decreased the amount they smoked since coming to college were more likely to want to quit "very much." Females and students in early college years were more likely to be planning to quit before graduation, as were students who had decreased the amount they smoked since coming to college. Interventions should target students who are in their early college years, given that habits prior to college, changes in smoking habits while in college, and year in college are associated with students' self-identification as a regular smoker, desire to quit smoking, or plan to quit smoking while in college.
Descriptors
Adult, Female, Health Knowledge, Attitudes, Practice, Humans, Idaho/epidemiology, Life Style, Male, Oregon/epidemiology, Peer Group, Population Surveillance, Risk Factors, Self Concept, Self Efficacy, Sex Distribution, Smoking/epidemiology/psychology, Smoking Cessation/psychology/statistics & numerical data, Students/psychology/statistics & numerical data, Tobacco Use Disorder/epidemiology/psychology, Universities/organization & administration, Washington/epidemiology
Links
Book Title
Database
Publisher
Data Source
Authors
Harris,J. B., Schwartz,S. M., Thompson,B.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Differences in prevalence of tobacco use among Indian urban youth: the role of socioeconomic status 2008 Division of Epidemiology and Community Health, School of Public Health, University of Minnesota, Minneapolis, MN, USA. math0304@umn.edu
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco
Periodical, Abbrev.
Nicotine Tob.Res.
Pub Date Free Form
Jan
Volume
10
Issue
1
Start Page
109
Other Pages
116
Notes
LR: 20151119; GR: R01 TW005952/TW/FIC NIH HHS/United States; GR: R01TW05952-01/TW/FIC NIH HHS/United States; JID: 9815751; ppublish
Place of Publication
England
ISSN/ISBN
1462-2203; 1462-2203
Accession Number
PMID: 18188751
Language
eng
SubFile
Journal Article; Research Support, N.I.H., Extramural; IM
DOI
10.1080/14622200701767779 [doi]
Output Language
Unknown(0)
PMID
18188751
Abstract
This study examined whether the distribution of tobacco use and related psychosocial risk factors among youth in urban India vary by socioeconomic status (SES). Data were derived from a cross-sectional survey of students enrolled in the 6th and 8th grades in 32 schools in Delhi and Chennai (N = 11,642). The survey was conducted in 2004, before the implementation of a program designed to prevent and reduce tobacco use (MYTRI). Mixed-effect regression models were used (a) to determine the prevalence of tobacco use among private (higher SES) and government (lower SES) school students, (b) to investigate whether certain psychosocial factors were associated with increased tobacco use, and (c) to determine how these factors varied by school type. Ever-use of multiple forms of tobacco (e.g., gutkha, bidis, and cigarettes) was more prevalent among government school students than private school students. After adjusting for city, gender, grade, and age, we found the prevalence rate for ever-use of any tobacco product to be 18.9% for government school students, compared with 12.2% for private school students (p<.01). Students in government schools scored lower than private school students on most psychosocial risk factors for tobacco use studied here, indicating higher risk. Government school students scored the lowest for refusal skills, self-efficacy, and reasons not to use tobacco. Social susceptibility to chewing tobacco and social susceptibility to smoking were strong correlates of current tobacco use among government school students. Exposure to tobacco advertising was also a strong correlate of current tobacco use for government school students but not private school students. In two large cities of India, students attending government schools are using many forms of tobacco at higher rates than private school students. The psychosocial risk profile of government school students suggests they are more vulnerable to initiation and use and to outside influences that encourage use.
Descriptors
Adolescent, Adolescent Behavior/ethnology/psychology, Cross-Sectional Studies, Female, Health Knowledge, Attitudes, Practice, Humans, India/epidemiology, Male, Peer Group, Poverty, Prevalence, Self Efficacy, Smoking/epidemiology, Social Class, Social Environment, Students/statistics & numerical data, Surveys and Questionnaires, Tobacco Use Disorder/epidemiology, Urban Population/statistics & numerical data
Links
Book Title
Database
Publisher
Data Source
Authors
Mathur,C., Stigler,M. H., Perry,C. L., Arora,M., Reddy,K. S.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Molecular monitoring of bacterial community structure in long-aged nukadoko: pickling bed of fermented rice bran dominated by slow-growing lactobacilli 2007 Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. nakayama@agr.kyushu-u.ac.jp
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of bioscience and bioengineering
Periodical, Abbrev.
J.Biosci.Bioeng.
Pub Date Free Form
Dec
Volume
104
Issue
6
Start Page
481
Other Pages
489
Notes
LR: 20151119; JID: 100888800; 2007/05/28 [received]; 2007/09/18 [accepted]; ppublish
Place of Publication
Japan
ISSN/ISBN
1389-1723; 1347-4421
Accession Number
PMID: 18215635
Language
eng
SubFile
Journal Article; IM
DOI
10.1263/jbb.104.481 [doi]
Output Language
Unknown(0)
PMID
18215635
Abstract
Nukadoko is the fermented rice bran bed traditionally used for pickling vegetables in Japan. Here, we investigate the bacterial community structure of nukadoko using several culture-independent methods. Denaturing gradient gel electrophoresis (DGGE) and sequence analysis of V2-V3 16S rRNA gene (16S rDNA) fragments amplified from a long-aged nukadoko bacterial community indicated seven predominant operational taxonomic units (OTUs) closely related to known Lactobacillus species. Phylogenetic analysis of these OTUs indicated a major cluster consisting of six OTUs including a dominant OTU closely related to Lactobacillus acidifarinae and one distinct OTU corresponding to Lactobacillus acetotolerans. L. acetotolerans was commonly detected as a dominant species in samples from different seasons. The succession of microbial community structure in the fermentation and ripening processes was investigated using a laboratory model nukadoko. The L. acidifarinae-like bacteria grew rapidly with a pH decrease in the first few days after inoculation, whereas L. acetotolerans grew slowly and became dominant after one week. Real-time quantitative polymerase chain reaction (Q-PCR) showed that the doubling time of L. acetotolerans was 12 h, while that of total bacteria was 4 h. Real-time quantitative reverse transcription polymerase chain reaction (Q-RT-PCR) targeting 16S rRNA showed a low metabolic activity of L. acetotolerans throughout the fermentation and ripening processes. Fluorescence in situ hybridization (FISH) showed that L. acetotolerans was a dominant bacterium in the ripening period and had a low metabolic activity. These results indicate that the slow-growing L. acetotolerans stably dominated nukadoko microbiota after the L. acidifarinae-like bacteria mainly contributed to the lactic acid fermentation of the rice bran.
Descriptors
Colony Count, Microbial/methods, Fermentation, Lactobacillus/classification/genetics/isolation & purification, Oryza/microbiology, Reverse Transcriptase Polymerase Chain Reaction/methods
Links
Book Title
Database
Publisher
Data Source
Authors
Nakayama,J., Hoshiko,H., Fukuda,M., Tanaka,H., Sakamoto,N., Tanaka,S., Ohue,K., Sakai,K., Sonomoto,K.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Global youth tobacco surveillance, 2000-2007 2008 Office on Smoking and Health, National Center for Chronic Disease Prevention and Health Promotion, CDC, Atlanta, GA 30341, USA. wcw1@cdc.gov
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Morbidity and mortality weekly report.Surveillance summaries (Washington, D.C.: 2002)
Periodical, Abbrev.
MMWR Surveill.Summ.
Pub Date Free Form
25-Jan
Volume
57
Issue
1
Start Page
1
Other Pages
28
Notes
LR: 20141120; JID: 101142015; ppublish
Place of Publication
United States
ISSN/ISBN
1545-8636; 0892-3787
Accession Number
PMID: 18219269
Language
eng
SubFile
Journal Article; IM
DOI
ss5701a1 [pii]
Output Language
Unknown(0)
PMID
18219269
Abstract
PROBLEM: Tobacco use is a major contributor to deaths from chronic diseases. The findings from the Global Youth Tobacco Survey (GYTS) suggest that the estimate of a doubling of deaths from smoking (from 5 million per year to approximately 10 million per year by 2020) might be an underestimate because of the increase in smoking among young girls compared with adult females, the high susceptibility of smoking among never smokers, high levels of exposure to secondhand smoke, and protobacco indirect advertising. REPORTING PERIOD COVERED: This report includes GYTS data collected during 2000-2007 from 140 World Health Organization (WHO) member states, six territories (American Samoa, British Virgin Islands, Guam, Montserrat, Puerto Rico, and the U.S. Virgin Islands), two geographic regions (Gaza Strip and West Bank), one United Nations administered province (Kosovo), one special administrative region (Macau), and one Commonwealth (Northern Mariana Islands). For countries that have repeated GYTS, only the most recent data are included. For countries with multiple survey sites, only data from the capital or largest city are presented. DESCRIPTION OF SYSTEM: GYTS is a school-based survey of a defined geographic site that can be a country, a province, a city, or any other geographic entity. GYTS uses a standardized methodology for constructing sampling frames, selecting schools and classes, preparing questionnaires, conducting field procedures, and processing data. GYTS standard sampling methodology uses a two-stage cluster sample design that produces samples of students in grades associated with students aged 13-15 years. Each sampling frame includes all schools (usually public and private) in a geographically defined area containing any of the identified grades. In the first stage, the probability of schools being selected is proportional to the number of students enrolled in the specified grades. In the second sampling stage, classes within the selected schools are selected randomly. All students in selected classes attending school the day the survey is administered are eligible to participate. Student participation is voluntary and anonymous using self-administered data collection procedures. The GYTS sample design produces independent, cross-sectional estimates that are representative of each site. RESULTS: The findings in this report indicate that the level of cigarette smoking between boys and girls is similar in many sites; the prevalence of cigarette smoking and use of other tobacco products is similar; and susceptibility to initiate smoking among never smokers is similar among boys and girls and is higher than cigarette smoking in the majority of sites. Approximately half of the students reported that they were exposed to secondhand smoke in public places during the week preceding the survey. Approximately eight in 10 favor a ban on smoking in public places. Approximately two in 10 students own an object with a cigarette brand logo on it, and one in 10 students have been offered free cigarettes by a tobacco company representative. Approximately seven in 10 students who smoke reported that they wanted to stop smoking. Approximately seven in 10 students who smoked were not refused purchase of cigarettes from a store during the month preceding the survey. Finally, approximately six in 10 students reported having been taught in school about the harmful effects of smoking during the year preceding the survey. INTERPRETATION: The findings in this report suggest that interventions that decrease tobacco use among youth (e.g., increasing excise taxes, media campaigns, school programs in conjunction with community interventions, and community interventions that decrease minors' access to tobacco) must be broad-based, focused on boys and girls, and have components directed toward prevention and cessation. If effective programs are not developed and implemented soon, future morbidity and mortality attributed to tobacco probably will
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Adolescent, Female, Global Health, Humans, Male, Population Surveillance, Smoking/epidemiology/trends, World Health Organization
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Warren,C. W., Jones,N. R., Peruga,A., Chauvin,J., Baptiste,J. P., Costa de Silva,V., el Awa,F., Tsouros,A., Rahman,K., Fishburn,B., Bettcher,D. W., Asma,S., Centers for Disease Control and Prevention (CDC)
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