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Role of discontinuous chlorination on microbial production by drinking water biofilms 2005 Lab. Health & Environmental Microbiology, Polytechnical University of Catalonia, Terrassa-08222, Barcelona, Spain.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Water research
Periodical, Abbrev.
Water Res.
Pub Date Free Form
May
Volume
39
Issue
9
Start Page
1896
Other Pages
1906
Notes
LR: 20131121; JID: 0105072; 4R7X1O2820 (Chlorine); 2003/11/19 [received]; 2004/09/16 [revised]; 2005/02/21 [accepted]; 2005/04/26 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
0043-1354; 0043-1354
Accession Number
PMID: 15899288
Language
eng
SubFile
Journal Article; IM
DOI
S0043-1354(05)00107-7 [pii]
Output Language
Unknown(0)
PMID
15899288
Abstract
Microbial quality in water distribution systems is strongly affected by the development of microbial biofilms. Production and release of microbial cells by the biofilm affect microbial levels in the water column and in some cases this fact constitutes a public health concern. In this study, we attempt to analyze in which way the existence of different episodes of chlorine depletion affects both biofilm formation and microbial load of an artificial laboratory system. The work was carried out using two parallel packed bed reactors both supplied with running tap water. One of the reactors was used as a control and was permanently exposed to the action of chlorine. In the other reactor, chlorine was neutralized at selected times during the experiment and for periods of variable length. During the experiment the concentration of total and viable cells from the effluent was monitored at the exit of each of the reactors. The data obtained were used to estimate microbial production from the biofilms. As an average, release of microbial cells to the water phase increased tenfold in the absence of chlorine. The results also indicate that disinfectant efficiency against the biofilm was not recovered when chlorine returned to normal levels after each event of chlorine neutralization. Cell viability in the water phase in the presence of chlorine was low at the beginning of the experiment but increased 4 orders of magnitude after five neutralization periods. Therefore, subsequent episodes of chlorine depletion may accelerate the development of microbial communities with reduced susceptibility to disinfection in real drinking water systems.
Descriptors
Biofilms/growth & development, Bioreactors, Chlorine/analysis/chemistry, Colony Count, Microbial, Water Microbiology, Water Purification/methods, Water Supply
Links
Book Title
Database
Publisher
Data Source
Authors
Codony,F., Morato,J., Mas,J.
Original/Translated Title
URL
Date of Electronic
20050426
PMCID
Editors
A single air dive reduces arterial endothelial function in man 2005 Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway. alfb@ntnu.no
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
The Journal of physiology
Periodical, Abbrev.
J.Physiol.
Pub Date Free Form
1-Aug
Volume
566
Issue
Pt 3
Start Page
901
Other Pages
906
Notes
LR: 20140606; JID: 0266262; 0 (Gases); OID: NLM: PMC1464788; 2005/06/16 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
0022-3751; 0022-3751
Accession Number
PMID: 15961424
Language
eng
SubFile
Clinical Trial; Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
jphysiol.2005.089862 [pii]
Output Language
Unknown(0)
PMID
15961424
Abstract
During and after decompression from dives, gas bubbles are regularly observed in the right ventricular outflow tract. A number of studies have documented that these bubbles can lead to endothelial dysfunction in the pulmonary artery but no data exist on the effect of diving on arterial endothelial function. The present study investigated if diving or oxygen breathing would influence endothelial arterial function in man. A total of 21 divers participated in this study. Nine healthy experienced male divers with a mean age of 31 +/- 5 years were compressed in a hyperbaric chamber to 280 kPa at a rate of 100 kPa min(-1) breathing air and remaining at pressure for 80 min. The ascent rate during decompression was 9 kPa min(-1) with a 7 min stop at 130 kPa (US Navy procedure). Another group of five experienced male divers (31 +/- 6 years) breathed 60% oxygen (corresponding to the oxygen tension of air at 280 kPa) for 80 min. Before and after exposure, endothelial function was assessed in both groups as flow-mediated dilatation (FMD) by ultrasound in the brachial artery. The results were compared to data obtained from a group of seven healthy individuals of the same age who had never dived. The dive produced few vascular bubbles, but a significant arterial diameter increase from 4.5 +/- 0.7 to 4.8 +/- 0.8 mm (mean +/- s.d.) and a significant reduction of FMD from 9.2 +/- 6.9 to 5.0 +/- 6.7% were observed as an indication of reduced endothelial function. In the group breathing oxygen, arterial diameter increased significantly from 4.4 +/- 0.3 mm to 4.7 +/- 0.3 mm, while FMD showed an insignificant decrease. Oxygen breathing did not decrease nitroglycerine-induced dilatation significantly. In the normal controls the arterial diameter and FMD were 4.1 +/- 0.4 mm and 7.7 +/- 0.2.8%, respectively. This study shows that diving can lead to acute arterial endothelial dysfunction in man and that oxygen breathing will increase arterial diameter after return to breathing air. Further studies are needed to determine if these mechanisms are involved in tissue injury following diving.
Descriptors
Adult, Decompression Sickness/etiology/prevention & control, Diving/adverse effects/physiology, Endothelium, Vascular/physiology, Gases/blood, Humans, Male, Pulmonary Artery/physiology
Links
Book Title
Database
Publisher
Data Source
Authors
Brubakk,A. O., Duplancic,D., Valic,Z., Palada,I., Obad,A., Bakovic,D., Wisloff,U., Dujic,Z.
Original/Translated Title
URL
Date of Electronic
20050616
PMCID
PMC1464788
Editors
Analysis of polycyclic aromatic hydrocarbons in wastewater treatment plant effluents using hollow fibre liquid-phase microextraction 2005 Department of Environmental Engineering, Technical University of Crete, Polytechneioupolis, University Campus, GR-73100, Chania, Crete, Greece.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Chemosphere
Periodical, Abbrev.
Chemosphere
Pub Date Free Form
Jul
Volume
60
Issue
5
Start Page
690
Other Pages
698
Notes
LR: 20091119; JID: 0320657; 0 (Polycyclic Hydrocarbons, Aromatic); 0 (Water Pollutants, Chemical); 2004/02/17 [received]; 2004/12/22 [revised]; 2005/01/12 [accepted]; 2005/03/03 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
0045-6535; 0045-6535
Accession Number
PMID: 15963807
Language
eng
SubFile
Journal Article; IM
DOI
S0045-6535(05)00188-8 [pii]
Output Language
Unknown(0)
PMID
15963807
Abstract
A simple and efficient method for the enrichment of low molecular weight polycyclic aromatic hydrocarbons (PAHs) in effluents originating from wastewater treatment plants is presented here. The proposed protocol couples the recently introduced hollow fibre liquid-phase microextraction (LPME) method with gas chromatography-mass spectrometry. Method parameters were controlled and the optimised experimental conditions were: 5 ml aqueous samples, containing 2.5% NaCl w/v, stirred at 1000 rpm, extracted with toluene for 15 min. The developed protocol yielded a linear calibration curve in the concentration range from 0.5 to 50 microg l(-1) for all target analytes (namely acenaphthene, phenanthrene, fluoranthene and pyrene) and limits of detection in the low microg l(-1) level (0.005-0.011 microg l(-1)). The repeatability and inter-day precision of the method varied between 2.7% and 11.3% and 7.9% and 14.4% respectively. The relative recoveries from different types of natural water samples revealed that matrix had a small effect on the hollow fibre LPME process. The developed method was then applied for the determination of PAHs contamination in effluent samples taken from two major municipal wastewater treatment plants. The results were compared with those obtained with solid-phase microextraction. The ability of both microextraction methods to concentrate organic analytes was demonstrated as both methods confirmed the presence of PAHs as well as of phthalates in the examined effluent samples.
Descriptors
Chemistry Techniques, Analytical/methods, Environmental Monitoring/instrumentation/methods, Equipment Design, Gas Chromatography-Mass Spectrometry, Polycyclic Hydrocarbons, Aromatic/analysis, Waste Disposal, Fluid, Water Pollutants, Chemical/analysis
Links
Book Title
Database
Publisher
Data Source
Authors
Charalabaki,M., Psillakis,E., Mantzavinos,D., Kalogerakis,N.
Original/Translated Title
URL
Date of Electronic
20050303
PMCID
Editors
Impact of RO-desalted water on distribution water qualities 2005 Civil and Environmental Engineering Department, University of Central Florida, Orlando, FL 32816, USA. taylor@mail.ucf.edu
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Water science and technology : a journal of the International Association on Water Pollution Research
Periodical, Abbrev.
Water Sci.Technol.
Pub Date Free Form
Volume
51
Issue
7-Jun
Start Page
285
Other Pages
291
Notes
LR: 20131121; JID: 9879497; 0 (Chlorides); 0 (Metals); 0 (Salts); 0 (Sulfates); E1UOL152H7 (Iron); H0G9379FGK (Calcium Carbonate); ppublish
Place of Publication
England
ISSN/ISBN
0273-1223; 0273-1223
Accession Number
PMID: 16003988
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
Output Language
Unknown(0)
PMID
16003988
Abstract
A large-scale pilot distribution study was conducted to investigate the impacts of blending different source waters on distribution water qualities, with an emphasis on metal release (i.e. corrosion). The principal source waters investigated were conventionally treated ground water (G1), surface water processed by enhanced treatment (S1), and desalted seawater by reverse osmosis membranes (RO). Due to the nature of raw water quality and associated treatment processes, G1 water had high alkalinity, while S1 and RO sources were characterized as high sulfate and high chloride waters, respectively. The blending ratio of different treated waters determined the quality of finished waters. Iron release from aged cast iron pipes increased significantly when exposed to RO and S1 waters: that is, the greater iron release was experienced with alkalinity reduced below the background of G1 water. Copper release to drinking water, however, increased with increasing alkalinity and decreasing pH. Lead release, on the other hand, increased with increasing chloride and decreasing sulfate. The effect of pH and alkalinity on lead release was not clearly observed from pilot blending study. The flat and compact corrosion scales observed for lead surface exposed to S1 water may be attributable to lead concentration less than that of RO water blends.
Descriptors
Calcium Carbonate/chemistry, Chlorides/chemistry, Corrosion, Filtration, Fresh Water/chemistry, Hydrogen-Ion Concentration, Iron/chemistry, Membranes, Metals/chemistry, Osmosis, Salts/isolation & purification, Seawater/chemistry, Sulfates/chemistry, Water Purification/methods, Water Supply/standards
Links
Book Title
Database
Publisher
Data Source
Authors
Taylor,J., Dietz,J., Randall,A., Hong,S.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Inner ear decompression sickness following a scuba dive 1992 Department of Otolaryngology, National Defense Medical College.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Nihon Jibiinkoka Gakkai kaiho
Periodical, Abbrev.
Nihon Jibiinkoka Gakkai.Kaiho
Pub Date Free Form
Apr
Volume
95
Issue
4
Start Page
499
Other Pages
504
Notes
LR: 20110728; JID: 7505728; ppublish
Place of Publication
JAPAN
ISSN/ISBN
0030-6622; 0030-6622
Accession Number
PMID: 1602351
Language
jpn
SubFile
Case Reports; English Abstract; Journal Article; IM
DOI
Output Language
Unknown(0)
PMID
1602351
Abstract
Inner ear decompression sickness (IEDCS) is one form of Type II decompression sickness. Most cases of IEDCS have been associated with saturation dives, so there are very few reports of occurrence following shallow scuba dives. We present here the case of a diver who suffered from IEDCS following a shallow scuba dive (30m), and was successfully treated by the protocol outlined in U.S. Navy treatment table 6. This case suggests that there is the possibility of occurrence of IEDCS, even following a shallow scuba dive, if proper decompression procedures are not adhered to. In addition, detailed analysis of diving profiles should be used to distinguish the inner ear dysfunction seen in some divers from inner ear barotrauma which may be attributable to IEDCS.
Descriptors
Adult, Decompression Sickness/etiology, Diving/injuries, Ear, Inner/injuries, Humans, Male
Links
Book Title
Database
Publisher
Data Source
Authors
Satoh,M., Kitahara,S., Inouye,T., Ikeda,T.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Anti-metabolic activity of caspofungin against Candida albicans and Candida parapsilosis biofilms 2005 Laboratoire de Parasitologie et Mycologie Medicales, Centre Hospitalier Universitaire La Miletrie, BP 577, 86021 Poitiers Cedex, France.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
The Journal of antimicrobial chemotherapy
Periodical, Abbrev.
J.Antimicrob.Chemother.
Pub Date Free Form
Sep
Volume
56
Issue
3
Start Page
507
Other Pages
512
Notes
LR: 20131121; JID: 7513617; 0 (Antifungal Agents); 0 (Antimetabolites); 0 (Echinocandins); 0 (Peptides, Cyclic); 8VZV102JFY (Fluconazole); F0XDI6ZL63 (caspofungin); 2005/07/22 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
0305-7453; 0305-7453
Accession Number
PMID: 16040622
Language
eng
SubFile
Journal Article; IM
DOI
dki269 [pii]
Output Language
Unknown(0)
PMID
16040622
Abstract
OBJECTIVES: Candidiasis can be associated with the formation of biofilms on bioprosthetic surfaces and the intrinsic resistance of Candida albicans biofilms to the most commonly used antifungal agents has been demonstrated. In this study, we report on the antifungal activity of caspofungin at two different concentrations, on C. albicans and Candida parapsilosis biofilms with different ages of maturation. METHODS: Fifteen strains of C. albicans (10 strains susceptible to fluconazole in vitro and five strains resistant to this antifungal agent) and six strains of C. parapsilosis (all were susceptible to fluconazole in vitro) were studied. The antifungal activity of caspofungin was assessed by looking for a significant inhibition of the metabolic activity of yeasts within biofilms. Biofilms of Candida were produced in vitro, on silicone catheters. RESULTS: Caspofungin used at MIC did not modify the metabolic activity of C. albicans, whatever the maturation age of the biofilms. The same concentration of caspofungin significantly reduced the metabolism (P<or=0.001) of 25% (biofilms of 48 h) to 50% (biofilms of 2 h) of the C. parapsilosis yeasts. The use of a therapeutic concentration of caspofungin (2 mg/L) significantly decreased (P<or=0.001) the metabolism of all the strains of C. albicans and C. parapsilosis tested, independently of the biofilm maturation age. This potent antifungal activity of caspofungin on C. albicans biofilms was observed independently of the yeast susceptibility to fluconazole. CONCLUSIONS: This study demonstrated that caspofungin used at MIC was not sufficient to reduce C. albicans biofilms, but it suggested an activity on C. parapsilosis biofilms depending on their maturation age. This study also indicated that caspofungin used at 2 mg/L could be a good candidate in the prevention of candidiasis associated with silicone medical devices. Our results also suggested that fluconazole resistance of yeasts did not affect caspofungin activity.
Descriptors
Antifungal Agents/pharmacology, Antimetabolites/pharmacology, Biofilms/drug effects/growth & development, Candida/drug effects/physiology, Candida albicans, Dose-Response Relationship, Drug, Drug Resistance, Multiple, Fungal, Echinocandins, Fluconazole/pharmacology, Microbial Sensitivity Tests, Peptides, Cyclic/pharmacology
Links
Book Title
Database
Publisher
Data Source
Authors
Cocuaud,C., Rodier,M. H., Daniault,G., Imbert,C.
Original/Translated Title
URL
Date of Electronic
20050722
PMCID
Editors
Trace analysis of polycyclic aromatic hydrocarbons in suspended particulate matter by accelerated solvent extraction followed by gas chromatography-mass spectrometry 2005 Institute for Environment and Sustainability (IES), Inland and Marine Waters Unit, Joint Research Centre of the European Commission, 21020 Ispra (VA), Italy. angels.olivella@terra.es
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Analytical and bioanalytical chemistry
Periodical, Abbrev.
Anal.Bioanal Chem.
Pub Date Free Form
Sep
Volume
383
Issue
1
Start Page
107
Other Pages
114
Notes
LR: 20160512; JID: 101134327; 0 (Anthracenes); 0 (Hexanes); 0 (Naphthalenes); 0 (Polycyclic Hydrocarbons, Aromatic); 0 (Solvents); 0 (Water Pollutants, Chemical); 1364PS73AF (Acetone); 2166IN72UN (naphthalene); EH46A1TLD7 (anthracene); 2005/03/14 [receive
Place of Publication
Germany
ISSN/ISBN
1618-2642; 1618-2642
Accession Number
PMID: 16041602
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1007/s00216-005-3383-1 [doi]
Output Language
Unknown(0)
PMID
16041602
Abstract
An analytical procedure based on extraction by accelerated solvent extraction (ASE) followed by gas chromatography-mass spectrometry (GC/MS) analysis has been developed for the determination of particulate polycyclic aromatic hydrocarbons (PAHs) from large-volume water samples (20 L). The effect of temperature and number of cycles on the efficiency of ASE was investigated: the best results were obtained by using a temperature of 100 degrees C and one static cycle. A mixture of hexane/acetone 1:1 (v/v) was used as extraction solvent. Mean total method recovery under optimized conditions was 85%. The developed methodology was applied to the analysis of suspended particulate matter from Lake Maggiore waters (north of Italy). Mean PAH concentrations in suspended particulate matter from Lake Maggiore ranged from 0.2 ng L(-1) for anthracene to 18.7 ng L(-1) for naphthalene.
Descriptors
Acetone/chemistry, Anthracenes/analysis, Fresh Water/analysis, Gas Chromatography-Mass Spectrometry/methods, Hexanes/chemistry, Naphthalenes/analysis, Polycyclic Hydrocarbons, Aromatic/analysis, Solvents/chemistry, Temperature, Water Pollutants, Chemical/analysis
Links
Book Title
Database
Publisher
Data Source
Authors
Olivella,M. A.
Original/Translated Title
URL
Date of Electronic
20051019
PMCID
Editors
Identification of predominant lactic acid bacteria isolated from traditionally fermented vegetable products of the Eastern Himalayas 2005 Department of Botany, Sikkim Government College, Gangtok, Sikkim 737 102, India.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International journal of food microbiology
Periodical, Abbrev.
Int.J.Food Microbiol.
Pub Date Free Form
15-Dec
Volume
105
Issue
3
Start Page
347
Other Pages
356
Notes
LR: 20061115; JID: 8412849; 2004/10/05 [received]; 2005/01/22 [revised]; 2005/04/20 [accepted]; 2005/08/01 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
0168-1605; 0168-1605
Accession Number
PMID: 16055218
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
S0168-1605(05)00335-1 [pii]
Output Language
Unknown(0)
PMID
16055218
Abstract
Gundruk, sinki and khalpi are lactic-fermented vegetable products of Sikkim in India, and inziangsang is a fermented leafy vegetable product of Nagaland and Manipur in India. A total of 65 samples of gundruk (25), sinki (12), khalpi (25) and inziangsang (3) were analysed for microbial counts. The population of lactic acid bacteria (LAB) as well as aerobic mesophilic counts were at the level of 10(7) cfu g(-1). Yeasts were detected only in few samples of sinki and khalpi. No moulds were detected. In order to identify the predominating organisms, a total of 269 strains of LAB were isolated from gundruk, sinki, khalpi and inziangsang samples. The phenotypic characteristics of these strains were determined followed by genotyping using RAPD-PCR, repetitive element PCR and species-specific PCR techniques. The major representatives of the LAB involved in these fermentations were identified as Lactobacillus brevis, Lactobacillus plantarum, Pediococcus pentosaceus, Pediococcus acidilactici and Leuconostoc fallax.
Descriptors
Colony Count, Microbial, Fermentation, Food Microbiology, Genotype, Humans, India, Lactobacillus/classification/isolation & purification, Leuconostoc/classification/isolation & purification, Pediococcus/classification/isolation & purification, Phenotype, Phylogeny, Random Amplified Polymorphic DNA Technique, Vegetables/microbiology
Links
Book Title
Database
Publisher
Data Source
Authors
Tamang,J. P., Tamang,B., Schillinger,U., Franz,C. M., Gores,M., Holzapfel,W. H.
Original/Translated Title
URL
Date of Electronic
20050801
PMCID
Editors
Smoking habits, attitudes and smoking cessation among sixth-year medical students of Kaunas University of Medicine 2005 Institute for Biomedical Research, Kaunas University of Medicine, Kaunas, Lithuania. aurel@centras.lt
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Medicina (Kaunas, Lithuania)
Periodical, Abbrev.
Medicina (Kaunas)
Pub Date Free Form
Volume
41
Issue
7
Start Page
607
Other Pages
613
Notes
LR: 20151119; JID: 9425208; ppublish
Place of Publication
Lithuania
ISSN/ISBN
1648-9144; 1010-660X
Accession Number
PMID: 16062029
Language
lit
SubFile
Comparative Study; English Abstract; Journal Article; IM
DOI
0507-09 [pii]
Output Language
Unknown(0)
PMID
16062029
Abstract
The aim of the study was to assess trends of smoking and the relation between smoking behavior of undergraduate medical students and their attitudes towards smoking and treatment of tobacco dependence. METHODS: Data were collected from sixth-year medical students of Kaunas University of Medicine during ten consecutive years. A total of 1518 students completed the anonymous questionnaire; response rate was 83.5%. RESULTS: The daily smoking rate was 19.4% in males and 4.2% in females; 15.9% and 10.4% respectively were irregular smokers, never smoked 41.6% of males and 64% of females. 70% of smokers and 83% of nonsmokers agreed that tobacco smoking is one of the greatest health hazards of modern times; 97.1% of all students considered passive smoking as harmful. More nonsmokers (70%) than smokers (5l%) were aware of addictive nature of tobacco, but there was a little difference in their opinion about support to smokers for quitting: 83.2% of smokers and 87.1% of nonsmokers stated that medical doctors should help smokers to give up smoking and 68% of them thought that doctors had not enough knowledge how to help. Less smokers (55%) than nonsmokers (87%) believed that smoking should be banned in hospitals. CONCLUSIONS: Prevalence of smoking was significantly lower among medical students than in general population. There were no significant changes in smoking prevalence among male medical students during the period of 1995-2004. At the same time smoking prevalence among female medical students increased more than twice. Attitudes of smokers and nonsmokers were significantly different on most items of tobacco, but knowledge of tobacco problem in the undergraduate students was not sufficient. Teaching about tobacco and related issues remains essential in the undergraduate medical course.
Descriptors
Attitude to Health, Chi-Square Distribution, Female, Health Education, Humans, Lithuania/epidemiology, Male, Prevalence, Sex Factors, Smoking/epidemiology/prevention & control, Smoking Cessation, Students, Medical, Surveys and Questionnaires
Links
Book Title
Database
Publisher
Data Source
Authors
Veryga,A., Stanikas,T.
Original/Translated Title
Kauno medicinos universiteto Medicinos fakulteto studentu rukymo iprociai ir poziuris i rukymo kontrole
URL
Date of Electronic
PMCID
Editors
Bacterial community structure in kimchi, a Korean fermented vegetable food, as revealed by 16S rRNA gene analysis 2005 School of Biological Sciences, Seoul National University, 56-1 Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
International journal of food microbiology
Periodical, Abbrev.
Int.J.Food Microbiol.
Pub Date Free Form
15-Aug
Volume
103
Issue
1
Start Page
91
Other Pages
96
Notes
LR: 20061115; JID: 8412849; 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); 2004/03/30 [received]; 2004/06/04 [revised]; 2004/11/25 [accepted]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
0168-1605; 0168-1605
Accession Number
PMID: 16084269
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
S0168-1605(05)00076-0 [pii]
Output Language
Unknown(0)
PMID
16084269
Abstract
Kimchi is a traditional Korean food fermented from a variety of vegetables. We elucidated the microbial community structure of five commercially produced kimchis made from Chinese cabbage by examining culture-independent 16S rRNA gene clone libraries. Most of the clones (347 out of 348) belonged to lactic acid bacteria and included several species of the genera Lactobacillus, Leuconostoc and Weissella. Weissella koreensis was found in all the samples and predominated in three of them (42.6-82%). Leuconostoc gelidum, Leuconostoc gasicomitatum and Lactobacillus sakei were common in the remaining kimchi clone libraries (>34%). The composition of bacterial phylotypes in kimchi varied between samples. Our approach revealed different community structures from those reported in previous culture-dependent studies based on phenotypic identification methods. The culture-independent method used here proved to be efficient and accurate and showed that the bacterial communities in kimchi differ from those in other fermented vegetable foods.
Descriptors
Base Sequence, Brassica/microbiology, Colony Count, Microbial, Fermentation, Korea, Lactobacillus/classification/isolation & purification, Leuconostoc/classification/isolation & purification, Phylogeny, RNA, Bacterial/analysis, RNA, Ribosomal, 16S/analysis, Species Specificity
Links
Book Title
Database
Publisher
Data Source
Authors
Kim,M., Chun,J.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors