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Dissemination of cefotaxime-M-producing Escherichia coli isolates in poultry farms, but not swine farms, in China 2010 National Center for Surveillance of Antimicrobial Resistance, The State Food and Drug Administration, Chongwen District, Beijing, China.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Foodborne pathogens and disease
Periodical, Abbrev.
Foodborne Pathog.Dis.
Pub Date Free Form
Nov
Volume
7
Issue
11
Start Page
1387
Other Pages
1392
Notes
LR: 20131121; JID: 101120121; 0 (DNA, Bacterial); 5E8K9I0O4U (Ciprofloxacin); EC 3.5.2.6 (beta-Lactamases); N2GI8B1GK7 (Cefotaxime); 2010/06/29 [aheadofprint]; ppublish
Place of Publication
United States
ISSN/ISBN
1556-7125; 1535-3141
Accession Number
PMID: 20583961
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1089/fpd.2010.0581 [doi]
Output Language
Unknown(0)
PMID
20583961
Abstract
The objective of this study was to investigate the distribution of extended spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates in swine and poultry farms in China. Rectal or cloaca swabs of swine and chicken were collected from four province-level regions of China, and E. coli isolates were recovered and tested for antimicrobial susceptibility. The isolates producing ESBLs were further characterized by pulsed-field gel electrophoresis (PFGE) and sequence analysis of genes encoding beta -lactamases and class I integrons. In total, 156 and 224 E. coli isolates were recovered from rectal swabs of four swine farms and cloaca swabs of six chicken farms, respectively. Prevalence of resistant isolates was higher in chicken than in swine. Fifty-six isolates producing ESBLs were identified from chicken samples, but no ESBL-producing isolates were identified from swine samples. Of 56 ESBL-producing isolates, 54 isolates contained cefotaxime (CTX)-M type beta-lactamases, including bla(CTX-M-14) (n = 24), bla(CTX-M-65) (n = 13), bla(CTX-M-55) (n = 10), bla(CTX-M-24) (n = 3), bla(CTX-M-3) (n = 2), bla(CTX-M-15) (n = 1), and bla(CTX-M-64) (n = 1). Among 54 E. coli isolates containing bla(CTX-M), 11 PFGE clusters and 42 PFGE patterns were identified. More importantly, more than three-fourth of the ESBL-producing isolates in chicken were also resistant to ciprofloxacin. Our data demonstrated that chicken had become an important reservoir of bla(CTX-M) in China. Detailed molecular comparison of plasmids and genomes of isolates from various sources will help to better define the transmission dynamics of bla(CTX-M) between humans and food-producing animals.
Descriptors
Agriculture, Animals, Cefotaxime, China, Ciprofloxacin, DNA Fingerprinting, DNA, Bacterial/analysis, Drug Resistance, Bacterial/genetics, Electrophoresis, Gel, Pulsed-Field, Escherichia coli/enzymology/isolation & purification, Integrons/genetics, Microbial Sensitivity Tests, Poultry/microbiology, Swine/microbiology, beta-Lactamases/biosynthesis/genetics
Links
Book Title
Database
Publisher
Data Source
Authors
Li,J., Ma,Y., Hu,C., Jin,S., Zhang,Q., Ding,H., Ran,L., Cui,S.
Original/Translated Title
URL
Date of Electronic
20100629
PMCID
Editors
Distribution and antifungal susceptibility of Candida species causing nosocomial candiduria 2012 Department of Medical Microbiology, Akdeniz University Medical Faculty, Antalya, Turkey.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Medical mycology
Periodical, Abbrev.
Med.Mycol.
Pub Date Free Form
Jul
Volume
50
Issue
5
Start Page
529
Other Pages
532
Notes
LR: 20131213; JID: 9815835; 0 (Antifungal Agents); 8VZV102JFY (Fluconazole); 2011/10/11 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
1460-2709; 1369-3786
Accession Number
PMID: 21988703
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.3109/13693786.2011.618996 [doi]
Output Language
Unknown(0)
PMID
21988703
Abstract
The aim of the study was to investigate the distribution of Candida species isolated from urine specimens of hospitalized patients in Akdeniz University Hospital, Antalya, Turkey, as well as their susceptibilities to antifungal agents. A total of 100 patients who had nosocomial candiduria between March 2003 and May 2004 at the facility were included in the study. Organisms were identified by conventional methods and the use of API ID 32C strips. Susceptibilities of the isolates to amphotericin B were determined by Etest, whereas the minimum inhibitory concentration (MIC) values of these same strains to fluconazole, voriconazole and caspofungin were assessed using the broth microdilution method. The most common species recovered was C. albicans 44% of all yeasts, followed by C. tropicalis (20%), C. glabrata (18%), C. krusei (6%), C. famata (5%), C. parapsilosis (4%), C. kefyr (2%) and C. guilliermondii (1%). A total of nine (9%) of the isolates, including five C. krusei and four C. glabrata isolates were susceptible dose-dependent (SDD) to fluconazole. In constrast, only two C. glabrata and one C. krusei isolates were resistant to this antifungal. The voriconazole MICs for all Candida isolates were =0.5 mug/ml, except for one C. glabrata isolate with a MIC value of 2 mug/ml. Among all isolates, 94% were susceptible to amphotericin B with MIC values of
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Ozhak-Baysan,B., Ogunc,D., Colak,D., Ongut,G., Donmez,L., Vural,T., Gunseren,F.
Original/Translated Title
URL
Date of Electronic
20111011
PMCID
Editors
Distribution and molecular characterization of genes encoding CTX-M and AmpC beta-lactamases in Escherichia coli isolated from an Indian urban aquatic environment 2015 Microbial Pathogenicity Laboratory, Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India.; Microbial Pathogenicity Laboratory, Department of Microbiology, University of Delhi South Campus, Benito Juarez
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
The Science of the total environment
Periodical, Abbrev.
Sci.Total Environ.
Pub Date Free Form
1-Feb
Volume
505
Issue
Start Page
350
Other Pages
356
Notes
CI: Copyright (c) 2014; JID: 0330500; 0 (Bacterial Proteins); EC 3.5.2.- (beta-lactamase CTX-M-15); EC 3.5.2.6 (AmpC beta-lactamases); EC 3.5.2.6 (beta-Lactamases); OTO: NOTNLM; 2014/06/16 [received]; 2014/08/14 [revised]; 2014/09/25 [accepted]; 2014/10/2
Place of Publication
Netherlands
ISSN/ISBN
1879-1026; 0048-9697
Accession Number
PMID: 25461036
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.scitotenv.2014.09.084 [doi]
Output Language
Unknown(0)
PMID
25461036
Abstract
Aquatic environments harboring antibiotic resistant Escherichia coli constitute an important public health concern. Thus, it is important to characterize the resistance genetic elements of waterborne E. coli. It is also important to identify the predominant clonal groups/phylogroups represented by resistant strains to understand the epidemiology of antibiotic resistant E. coli in natural environments, and to identify the role of well-established genotypes in the spread of resistance in a particular geographical area through natural environments. In the present investigation, E. coli strains (n=126) isolated from various points along the river Yamuna traversing through the National Capital Territory of Delhi (India) were grouped phylogenetically. A collection of 61 strains representing all phylogroups was investigated for extended-spectrum beta-lactamase (ESBL) and AmpC production. blaTEM, blaSHV and blaCTX-M genes were detected and analyzed, promoter/attenuator mutations associated with chromosomally-mediated AmpC overexpression were identified, and plasmid-mediated ampC was determined. blaTEM was the most widespread (100%) gene followed by bla(CTX-M) (16%), and plasmid-mediated ampC (3%). bla(CTX-M-15) and bla(CMY-42) were identified as the genes encoding CTX-M type ESBL and CIT type AmpC beta-lactamases, respectively. CTX-M-15 ESBL phenotype was most common in phylogroup D (50%), followed by phylogroups B1 (30%), and A (20%). E. coli that produce plasmid-mediated AmpC were rare and present only in phylogroup D. Presence of multi beta-lactam resistance, bla(CTX-M-15) and bla(CMY-42) in waterborne E. coli belonging to virulence-associated phylogroup D highlights the need for routine surveillance of resistance determinants in aquatic environments. This is also the first report for the presence of bla(CMY-42) in waterborne E. coli.
Descriptors
Links
Book Title
Database
Publisher
Elsevier B.V
Data Source
Authors
Bajaj,P., Singh,N.S., Kanaujia,P.K., Virdi,J.S.
Original/Translated Title
URL
Date of Electronic
20141029
PMCID
Editors
Distribution of bacteria in a domestic hot water system in a Danish apartment building 2004 Albrechtsen, H.-J., Environmental and Resources DTU, Technical University of Denmark, Building 115, DK-2800 Lyngby, Denmark
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Water research
Periodical, Abbrev.
Water Res.
Pub Date Free Form
/
Volume
38
Issue
1
Start Page
225
Other Pages
235
Notes
Place of Publication
ISSN/ISBN
0043-1354
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
Bacterial growth in hot water systems seems to cause problems such as bad odor of the water, skin allergies and increased heat transfer resistance in heating coils. In order to establish a basis for long-term suppression of bacterial growth, we studied the distribution of bacteria in a Danish domestic hot water system. Heterotrophic plate counts (HPC) were measured in both water and biofilm samples from various sampling sites in the system. In hot water samples, where the temperature was 55-60°C, the HPC were 10 3-104CFU/mL at incubation temperatures of 25°C or 37°C and 105CFU/mL at 55°C or 65°C. In the cold water (10°C) supplying the hot water system, the HPC at 25°C or 37°C was lower than in the hot water, and no bacteria were found after incubation at 55°C or 65°C. HPC constituted from 38% to 84% of the AODC results in hot water but only 2% in cold water, which showed a high ratio of culturable bacteria in hot water. Biofilm samples from the hot water tank and the inner surface of the pipes in the cold and hot water distribution system were collected by specially designed sampling devices, which were exposed in the system for 42 days. The quasi-steady-state number of bacteria in the biofilm, measured as the geometric mean of the HPC obtained between 21 and 42 days, was five-fold higher in the hot water pipe (13×105CFU/cm 2 at 55°C) than in the cold water pipe (2.8×10 5CFU/cm2 at 25°C). There was no significant difference between the number of bacteria in the biofilm samples from the top, middle and bottom of the hot water tank, and the number of bacteria in the biofilm counted at 55°C ranged from 0.6×104 to 1.7×104CFU/cm2. The surfaces of the sacrificial aluminum anodes and the heating coils in the hot water tank also contained high bacterial numbers. The measured number of bacteria in water and biofilm samples was related to the dimensions of the hot water system, and calculations showed that the majority of bacteria (72%) were located in the biofilm especially in the distribution system, which accounts for the greatest surface area. Free-living bacteria accounted for 26% and only a minor part of the bacteria were in the sludge in the hot water tank (2%). © 2003 Elsevier Ltd. All rights reserved.
Descriptors
activated sludge, article, bacterial count, bacterial growth, bacterium contamination, bacterium culture, biofilm, calculation, geometry, heat transfer, high temperature, incubation time, low temperature, odor, priority journal, skin allergy, water sampling, water supply
Links
Book Title
Database
Embase; MEDLINE
Publisher
Data Source
Embase
Authors
Bagh,L. K., Albrechtsen,H. -J, Arvin,E., Ovesen,K.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Distribution of COPD-related symptoms in the Middle East and North Africa: results of the BREATHE study 2012 Ain Shams University, Cairo, Egypt.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Respiratory medicine
Periodical, Abbrev.
Respir.Med.
Pub Date Free Form
Dec
Volume
106 Suppl 2
Issue
Start Page
S25
Other Pages
32
Notes
CI: Copyright (c) 2012; JID: 8908438; ppublish
Place of Publication
England
ISSN/ISBN
1532-3064; 0954-6111
Accession Number
PMID: 23290701
Language
eng
SubFile
Journal Article; Multicenter Study; IM
DOI
10.1016/S0954-6111(12)70012-4 [doi]
Output Language
Unknown(0)
PMID
23290701
Abstract
Chronic obstructive pulmonary disease (COPD) is a major cause of morbidity and mortality worldwide. However, its epidemiology in many developing countries is poorly characterised. The objective of this analysis was to evaluate respiratory symptoms which could be COPD-related in a large sample of individuals aged >/= 40 years in ten countries in the Middle East and North Africa (Algeria, Egypt, Jordan, Lebanon, Morocco, Saudi Arabia, Syria, Tunisia, Turkey and United Arab Emirates), together with Pakistan, using a standardised methodology. A random sample of 457,258 telephone numbers was contacted. A screening questionnaire was administered to each eligible participant, which included six questions relating to respiratory symptoms. Of 65,154 eligible subjects, 62,086 agreed to participate and 61,551 provided usable data. The age- and gender-adjusted prevalence of symptoms (persistent productive cough or breathlessness or both) was 14.3% [95% CI: 14.0-14.6%], ranging from 7.2% in UAE to 19.1% in Algeria. Symptoms were more frequent (p /= 10 pack . years) was 3.6% [95% CI: 3.5-3.7%] (range: 1.9% in UAE to 6.1% in Syria). COPD was more frequent (p
Descriptors
Links
Book Title
Database
Publisher
Elsevier Ltd
Data Source
Authors
Tageldin,M.A., Nafti,S., Khan,J.A., Nejjari,C., Beji,M., Mahboub,B., Obeidat,N.M., Uzaslan,E., Sayiner,A., Wali,S., Rashid,N., El Hasnaoui,A., BREATHE Study Group
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Distribution of ether in two postmortem cases 2006 Phipps, R.A.J., Office of the Chief Medical Examiner, Baltimore, MD 21201, United States
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of analytical toxicology
Periodical, Abbrev.
J.Anal.Toxicol.
Pub Date Free Form
2006/
Volume
30
Issue
8
Start Page
635
Other Pages
637
Notes
Place of Publication
ISSN/ISBN
0146-4760; 0146-4760
Accession Number
Language
SubFile
DOI
Output Language
Unknown(0)
PMID
Abstract
Diethyl ether (ether) is a volatile liquid that was used in the 1800s as an anesthetic agent; however, it is no longer used for this purpose, partly because of its odor and flammability. Two postmortem cases in which ether was detected are presented. The first case was an 18-year-old male found hanging from a basement ceiling brace in a semi-sitting position with a gas mask covering his face. A container of PrestoneCurrency Sign starting fluid and a bong were found on the floor close to the body. The second case was a 20-year-old male found unresponsive in his dormitory room with two black plastic trash bags secured over his head. Two saturated rags and a resealable bag containing a clear liquid were contained within these trash bags. An almost empty can of TradcoCurrency Sign starting fluid was also found at the scene. Ether concentrations were determined by headspace gas chromatography - mass spectrometry in the selective ion monitoring mode. In case #1, the medical examiner ruled that the cause of death was asphyxia due to hanging; the manner of death was undetermined. In case #2, the medical examiner ruled that the cause of death was asphyxia and the manner of death was suicide.
Descriptors
ether, adult, article, asphyxia, autopsy, case report, cause of death, forensic medicine, gas chromatography, hanging, human, male, mass spectrometry, suicide
Links
Book Title
Database
Embase; MEDLINE
Publisher
Data Source
Embase
Authors
Cox,D., DeRienz,R., Phipps,R. A. J., Levine,B., Jacobs,A., Fowler,D.
Original/Translated Title
URL
http://docstore.ingenta.com/cgi-bin/ds_deliver/1/u/d/ISIS/32506016.1/pres/jat/2006/00000030/00000008/art00025/11D82806B958D6A41161142772AA1D167F500B0473.pdf?link=http://www.ingentaconnect.com/error/delivery&format=pdf
Date of Electronic
PMCID
Editors
Distribution of Legionella pneumophila bacteria and Naegleria and Hartmannella amoebae in thermal saline baths used in balneotherapy 2013 Department of Invertebrate Zoology, Faculty of Biology and Earth Sciences, Nicolaus Copernicus University, Torun, Poland. ezbikow@biol.uni.torun.pl
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Parasitology research
Periodical, Abbrev.
Parasitol.Res.
Pub Date Free Form
Jan
Volume
112
Issue
1
Start Page
77
Other Pages
83
Notes
LR: 20150222; JID: 8703571; 059QF0KO0R (Water); OID: NLM: PMC3536949; 2012/06/13 [received]; 2012/08/24 [accepted]; 2012/09/30 [aheadofprint]; ppublish
Place of Publication
Germany
ISSN/ISBN
1432-1955; 0932-0113
Accession Number
PMID: 23052757
Language
eng
SubFile
Journal Article; IM
DOI
10.1007/s00436-012-3106-4 [doi]
Output Language
Unknown(0)
PMID
23052757
Abstract
The present study was aimed at investigating the coexistence and interactions between free living amoebae of Naegleria and Hartmannella genera and pathogenic Legionella pneumophila bacteria in thermal saline baths used in balneotherapy in central Poland. Water samples were collected from November 2010 to May 2011 at intervals longer than 1 month. The microorganisms were detected with the use of a very sensitive fluorescence in situ hybridisation method. In addition, the morphology of the amoebae was studied. Despite relatively high salinity level, ranging from 1.5 to 5.0 %, L. pneumophila were found in all investigated baths, although their number never exceeded 10(6) cells dm(-3). Hartmannella were not detected, while Naegleria fowleri were found in one bath. The observation that N. fowleri and L. pneumophila may coexist in thermal saline baths is the first observation emphasising potential threat from these microorganisms in balneotherapy.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Zbikowska,E., Walczak,M., Krawiec,A.
Original/Translated Title
URL
Date of Electronic
20120930
PMCID
PMC3536949
Editors
Distribution system water age can create premise plumbing corrosion hotspots 2015 Via Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA, 24061, USA, smasters@vt.edu.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Environmental monitoring and assessment
Periodical, Abbrev.
Environ.Monit.Assess.
Pub Date Free Form
Sep
Volume
187
Issue
9
Start Page
559
Other Pages
015-4747-4. Epub 2015 Aug 7
Notes
JID: 8508350; 0 (Drinking Water); 0 (Water Pollutants, Chemical); 12597-71-6 (brass); 2P299V784P (Lead); 789U1901C5 (Copper); J41CSQ7QDS (Zinc); 2015/01/12 [received]; 2015/07/14 [accepted]; 2015/08/07 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
1573-2959; 0167-6369
Accession Number
PMID: 26251058
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; IM
DOI
10.1007/s10661-015-4747-4 [doi]
Output Language
Unknown(0)
PMID
26251058
Abstract
Cumulative changes in chemical and biological properties associated with higher "water age" in distribution systems may impact water corrosivity and regulatory compliance with lead and copper action levels. The purpose of this study was to examine the effects of water age and chemistry on corrosivity of various downstream premise plumbing pipe materials and configurations using a combination of controlled laboratory studies and a field survey. Examination of lead pipe, copper pipe with lead solder, and leaded brass materials in a replicated lab rig simulating premise plumbing stagnation events indicated that lead or copper release could increase as much as approximately 440 % or decrease as much as 98 % relative to water treatment plant effluent. In field studies at five utilities, trends in lead and copper release were highly dependent on circumstance; for example, lead release increased with water age in 13 % of cases and decreased with water age in 33 % of conditions tested. Levels of copper in the distribution system were up to 50 % lower and as much as 30 % higher relative to levels at the treatment plant. In many cases, high-risks of elevated lead and copper did not co-occur, demonstrating that these contaminants will have to be sampled separately to identify "worst case" conditions for human exposure and monitoring.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Masters,S., Parks,J., Atassi,A., Edwards,M.A.
Original/Translated Title
URL
Date of Electronic
20150807
PMCID
Editors
Diversity and technological potential of lactic acid bacteria of wheat flours 2013 Department of Agricultural and Forestry Science, University of Palermo, Palermo, Italy.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Food Microbiology
Periodical, Abbrev.
Food Microbiol.
Pub Date Free Form
Dec
Volume
36
Issue
2
Start Page
343
Other Pages
354
Notes
CI: Copyright (c) 2013; JID: 8601127; 0 (Acids); 0 (Bacterial Proteins); 33X04XA5AT (Lactic Acid); EC 3.4.- (Peptide Hydrolases); OTO: NOTNLM; 2013/01/08 [received]; 2013/06/29 [revised]; 2013/07/09 [accepted]; 2013/07/18 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
1095-9998; 0740-0020
Accession Number
PMID: 24010616
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.fm.2013.07.003 [doi]
Output Language
Unknown(0)
PMID
24010616
Abstract
Lactic acid bacteria (LAB) were analysed from wheat flours used in traditional bread making throughout Sicily (southern Italy). Plate counts, carried out in three different media commonly used to detect food and sourdough LAB, revealed a maximal LAB concentration of approximately 4.75 Log CFU g(-1). Colonies representing various morphological appearances were isolated and differentiated based on phenotypic characteristics and genetic analysis by randomly amplified polymorphic DNA (RAPD)-PCR. Fifty unique strains were identified. Analysis by 16S rRNA gene sequencing grouped the strains into 11 LAB species, which belonged to six genera: Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Pediococcus and Weissella. Weissella cibaria, Lactobacillus plantarum, Leuconostoc pseudomesenteroides and Leuconostoc citreum were the most prevalent species. The strains were not geographically related. Denaturing gradient gel electrophoresis (DGGE) analysis of total DNA of flour was used to provide a more complete understanding of the LAB population; it confirmed the presence of species identified with the culture-dependent approach, but did not reveal the presence of any additional LAB species. Finally, the technological characteristics (acidifying capacity, antimicrobial production, proteolytic activity, organic acid, and volatile organic compound generation) of the 50 LAB strains were investigated. Eleven strains were selected for future in situ applications.
Descriptors
Links
Book Title
Database
Publisher
Elsevier Ltd
Data Source
Authors
Alfonzo,A., Ventimiglia,G., Corona,O., Di Gerlando,R., Gaglio,R., Francesca,N., Moschetti,G., Settanni,L.
Original/Translated Title
URL
Date of Electronic
20130718
PMCID
Editors
Diversity of bacteria and yeast in the naturally fermented cotton seed and rice beverage produced by Brazilian Amerindians 2011 Departamento de Biologia, Universidade Federal de Lavras, Lavras, MG, Brazil.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Food Microbiology
Periodical, Abbrev.
Food Microbiol.
Pub Date Free Form
Oct
Volume
28
Issue
7
Start Page
1380
Other Pages
1386
Notes
LR: 20151119; CI: Copyright (c) 2011; JID: 8601127; 2011/01/07 [received]; 2011/06/11 [revised]; 2011/06/22 [accepted]; 2011/06/29 [aheadofprint]; ppublish
Place of Publication
England
ISSN/ISBN
1095-9998; 0740-0020
Accession Number
PMID: 21839389
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.fm.2011.06.012 [doi]
Output Language
Unknown(0)
PMID
21839389
Abstract
Microorganisms associated with the fermentation of cotton seed and rice were studied using a combination of culture-dependent and -independent methods. Samples of the cotton seed and rice beverage were collected every 8 h during the fermentation process for analysis of the microbiota present over 48 h. The lactic acid bacteria (LAB) population reached values of approximately 8.0 log cfu/mL. A total of 162 bacteria and 81 yeast isolates were identified using polyphasic methods. LAB (Lactobacillus plantarum, Lactobacillus vermiforme, Lactobacillus paracasei) were the most frequently isolated bacteria. Bacillus subtilis was present from 16 h until the end of the fermentation process. A decrease in pH value from 6.92 (0 h) to 4.76 (48 h) was observed, and the concentration of lactic acid reached 24 g/L at the end of the fermentation process. DGGE (denaturing gradient gel electrophoresis) was performed to determine the dynamics of the communities of bacteria and yeast, and the analysis revealed a predominance of LAB throughout the fermentation process. No changes were observed in the yeast community. The yeast species detected were Candida parapsilosis, Candida orthopsilosis, Clavispora lusitaniae and Rhodotorula mucilaginosa. Our studies indicate that the DGGE technique combined with a culture-dependent method is required to discern the dynamics in the fermentation of cotton seed and rice.
Descriptors
Links
Book Title
Database
Publisher
Elsevier Ltd
Data Source
Authors
Ramos,C.L., de Almeida,E.G., Freire,A.L., Freitas Schwan,R.
Original/Translated Title
URL
Date of Electronic
20110629
PMCID
Editors