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Isolation and identification of cultivable lactic acid bacteria in traditional yak milk products of Gansu Province in China 2012 Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University.
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Print(0)
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Journal Article
Periodical, Full
The Journal of general and applied microbiology
Periodical, Abbrev.
J.Gen.Appl.Microbiol.
Pub Date Free Form
Volume
58
Issue
2
Start Page
95
Other Pages
105
Notes
LR: 20131121; GENBANK/HM058717; GENBANK/HM058718; GENBANK/HM058719; GENBANK/HM058720; GENBANK/HM058721; GENBANK/HM058722; GENBANK/HM058723; GENBANK/HM058724; GENBANK/HM058725; GENBANK/HM058726; GENBANK/HM058727; GENBANK/HM058728; GENBANK/HM058729; GENBANK
Place of Publication
Japan
ISSN/ISBN
1349-8037; 0022-1260
Accession Number
PMID: 22688240
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
DN/JST.JSTAGE/jgam/58.95 [pii]
Output Language
Unknown(0)
PMID
22688240
Abstract
Various traditional fermented yak milk and raw milk foods could be considered as an abundant resource for obtaining novel lactic acid bacteria (LAB) with unique properties. Eighty-eight samples of yak milk products were collected from Gansu Province in China. Three hundred and nineteen strains of LAB isolated from these samples were identified by phenotypic methods, 16S rRNA gene sequence analysis and PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) technology. Among the isolates, one hundred and sixty-four isolates (51.41% of the total) were classified under Lactobacilli, and one hundred and fifty-five (48.59%) belonged to cocci. All the isolates were classified to six genera (Lactobacillus, Lactococcus, Leuconostoc, Streptococcus, Enterococcus and Weissella) and twenty-one species. Lactobacillus helveticus (87 strains), Leuconostoc mesenteroides subsp. mesenteroides (49 strains), Streptococcus thermophilus (39 strains), Lactobacillus casei (31 strains) and Lactococcus lactis subsp. lactis (19 strains) were considered as the predominant populations in the yak milk products. The results showed that there were abundant genus and species LAB existing in yak milk products in Gansu Province in China. The obtained LAB pure cultures may be a valuable source for further starter selection.
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Data Source
Authors
Bao,Q., Liu,W., Yu,J., Wang,W., Qing,M., Chen,X., Wang,F., Zhang,J., Zhang,W., Qiao,J., Sun,T., Zhang,H.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Investigating bacterial population structure and dynamics in traditional koumiss from Inner Mongolia using single molecule real-time sequencing 2016 Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Huhhot, 010018 China; Dairy Processing Laboratory of National Dairy Production Technology and Research Center, Huhhot, Inner Mongolia, Ch
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of dairy science
Periodical, Abbrev.
J.Dairy Sci.
Pub Date Free Form
10-Aug
Volume
Issue
Start Page
Other Pages
Notes
LR: 20160814; CI: Copyright (c) 2016; JID: 2985126R; OTO: NOTNLM; 2016/03/14 [received]; 2016/07/03 [accepted]; aheadofprint
Place of Publication
ISSN/ISBN
1525-3198; 0022-0302
Accession Number
PMID: 27522429
Language
ENG
SubFile
JOURNAL ARTICLE
DOI
S0022-0302(16)30539-2 [pii]
Output Language
Unknown(0)
PMID
27522429
Abstract
Koumiss is considered as a complete dairy product high in nutrients and with medicinal properties. The bacterial communities involved in production of koumiss play a crucial role in the fermentation cycle. To reveal bacterial biodiversity in koumiss and the dynamics of succession in bacterial populations during fermentation, 22 samples were collected from 5 sampling sites and the full length of the 16S ribosomal RNA genes sequenced using single molecule real-time sequencing technology. One hundred forty-eight species were identified from 82 bacterial genera and 8 phyla. These results suggested that the structural difference in the bacterial community could be attributed to geographical location. The most significant difference in bacterial composition occurred in samples from group D compared with other groups. The sampling location of group D was distant from the city and maintained the primitive local nomadic life. The dynamics of succession in bacterial communities showed that Lactobacillus helveticus increased in abundance from 0 to 9 h and reached its peak at 9 h and then decreased. In contrast, Enterococcus faecalis, Enterococcus durans, and Enterococcus casseliflavus increased gradually throughout the fermentation process, and reached a maximum after 24 h.
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Book Title
Database
Publisher
American Dairy Science Association. Published by Elsevier Inc
Data Source
Authors
Gesudu,Q., Zheng,Y., Xi,X., Hou,Q.C., Xu,H., Huang,W., Zhang,H., Menghe,B., Liu,W.
Original/Translated Title
URL
Date of Electronic
20160810
PMCID
Editors
Molecular identification and quantification of lactic acid bacteria in traditional fermented dairy foods of Russia 2015 Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P. R. China, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, P. R. China.; Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of dairy science
Periodical, Abbrev.
J.Dairy Sci.
Pub Date Free Form
Aug
Volume
98
Issue
8
Start Page
5143
Other Pages
5154
Notes
CI: Copyright (c) 2015; GENBANK/KC836519; GENBANK/KC836520; GENBANK/KC836521; GENBANK/KC836522; GENBANK/KC836523; GENBANK/KC836524; GENBANK/KC836525; GENBANK/KC836526; GENBANK/KC836527; GENBANK/KC836528; GENBANK/KC836529; GENBANK/KC836530; GENBANK/KC83653
Place of Publication
United States
ISSN/ISBN
1525-3198; 0022-0302
Accession Number
PMID: 26004836
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.3168/jds.2015-9460 [doi]
Output Language
Unknown(0)
PMID
26004836
Abstract
Russian traditional fermented dairy foods have been consumed for thousands of years. However, little research has focused on exploiting lactic acid bacteria (LAB) resources and analyzing the LAB composition of Russian traditional fermented dairy foods. In the present study, we cultured LAB isolated from fermented mare and cow milks, sour cream, and cheese collected from Kalmykiya, Buryats, and Tuva regions of Russia. Seven lactobacillus species and the Bifidobacterium genus were quantified by quantitative PCR. The LAB counts in these samples ranged from 3.18 to 9.77 log cfu/mL (or per gram). In total, 599 LAB strains were obtained from these samples using de Man, Rogosa, and Sharpe agar and M17 agar. The identified LAB belonged to 7 genera and 30 species by 16S rRNA and murE gene sequencing and multiplex PCR assay. The predominant LAB isolates were Lactobacillus helveticus (176 strains) and Lactobacillus plantarum (63 strains), which represented 39.9% of all isolates. The quantitative PCR results revealed that counts of 7 lactobacilli species and Bifidobacterium spp. of 30 fermented cow milk samples ranged from 1.19+/-0.34 (Lactobacillus helveticus in Tuva) to 8.09+/-0.71 (Lactobacillus acidophilus in Kalmykiya) log cfu/mL of fermented cow milk (mean +/- standard error). The numbers of Bifidobacterium spp., Lb. plantarum, Lb. helveticus, and Lb. acidophilus revealed no significant difference between the 3 regions; nevertheless, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus sakei, and Lactobacillus delbrueckii ssp. bulgaricus exhibited different degrees of variation across 3 regions. The results demonstrate that traditional fermented dairy products from different regions of Russia have complex compositions of LAB species. The diversity of LAB might be related to the type of fermented dairy product, geographical origin, and manufacturing process.
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Links
Book Title
Database
Publisher
American Dairy Science Association. Published by Elsevier Inc
Data Source
Authors
Yu,J., Wang,H.M., Zha,M.S., Qing,Y.T., Bai,N., Ren,Y., Xi,X.X., Liu,W.J., Menghe,B.L., Zhang,H.P.
Original/Translated Title
URL
Date of Electronic
20150523
PMCID
Editors
Diversity of lactic acid bacteria associated with traditional fermented dairy products in Mongolia 2011 Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P. R. China, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, P. R. China.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of dairy science
Periodical, Abbrev.
J.Dairy Sci.
Pub Date Free Form
Jul
Volume
94
Issue
7
Start Page
3229
Other Pages
3241
Notes
CI: Copyright (c) 2011; JID: 2985126R; 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); 2010/08/16 [received]; 2011/03/18 [accepted]; ppublish
Place of Publication
United States
ISSN/ISBN
1525-3198; 0022-0302
Accession Number
PMID: 21700007
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.3168/jds.2010-3727 [doi]
Output Language
Unknown(0)
PMID
21700007
Abstract
Spontaneous milk fermentation has a long history in Mongolia, and beneficial microorganisms have been handed down from one generation to the next for use in fermented dairy products. The objective of this study was to investigate the diversity of lactic acid bacteria (LAB) communities in fermented yak, mare, goat, and cow milk products by analyzing 189 samples collected from 13 different regions in Mongolia. The LAB counts in these samples varied from 3.41 to 9.03 log cfu/mL. Fermented yak and mare milks had almost identical mean numbers of LAB, which were significantly higher than those in fermented goat milk but slightly lower than those in fermented cow milk. In total, 668 isolates were obtained from these samples using de Man, Rogosa, and Sharpe agar and M17 agar. Each isolate was considered to be presumptive LAB based on gram-positive and catalase-negative properties, and was identified at the species level by 16S rRNA gene sequencing, multiplex PCR assay, and restriction fragment length polymorphism analysis. All isolates from Mongolian dairy products were accurately identified as Enterococcus faecalis (1 strain), Enterococcus durans (3 strains), Lactobacillus brevis (3 strains), Lactobacillus buchneri (2 strains), Lactobacillus casei (16 strains), Lactobacillus delbrueckii ssp. bulgaricus (142 strains), Lactobacillus diolivorans (17 strains), Lactobacillus fermentum (42 strains), Lactobacillus helveticus (183 strains), Lactobacillus kefiri (6 strains), Lactobacillus plantarum ssp. plantarum (7 strains), Lactococcus lactis ssp. lactis (7 strains), Leuconostoc lactis (22 strains), Leuconostoc mesenteroides (21 strains), Streptococcus thermophilus (195 strains), and Weissella cibaria (1 strain). The predominant LAB were Strep. thermophilus and Lb. helveticus, which were isolated from all sampling sites. The results demonstrate that traditional fermented dairy products from different regions of Mongolia have complex compositions of LAB species. Such diversity of LAB provides useful information for further studies of probiotic strain selection and starter culture design, with regard to the industrial production of traditional fermented milk.
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Links
Book Title
Database
Publisher
American Dairy Science Association. Published by Elsevier Inc
Data Source
Authors
Yu,J., Wang,W.H., Menghe,B.L., Jiri,M.T., Wang,H.M., Liu,W.J., Bao,Q.H., Lu,Q., Zhang,J.C., Wang,F., Xu,H.Y., Sun,T.S., Zhang,H.P.
Original/Translated Title
URL
Date of Electronic
PMCID
Editors
Identification of Coccoidal Bacteria in Traditional Fermented Milk Products from Mongolia, and the Fermentation Properties of the Predominant Species, Streptococcus thermophilus 2015 Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of P. R. China, Department of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China.; Key Laboratory of Dairy Biotechnology and Engineering, Edu
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Korean journal for food science of animal resources
Periodical, Abbrev.
Korean J.Food Sci.Anim.Resour.
Pub Date Free Form
Volume
35
Issue
5
Start Page
683
Other Pages
691
Notes
LR: 20160115; JID: 101660566; OID: NLM: PMC4670899; OTO: NOTNLM; 2015/05/19 [received]; 2015/09/25 [revised]; 2015/09/26 [accepted]; 2015/10/31 [epublish]; ppublish
Place of Publication
Korea (South)
ISSN/ISBN
1225-8563; 1225-8563
Accession Number
PMID: 26761898
Language
eng
SubFile
Journal Article
DOI
10.5851/kosfa.2015.35.5.683 [doi]
Output Language
Unknown(0)
PMID
26761898
Abstract
The objective of this study was to identify the coccoidal bacteria present in 188 samples of fermented yaks', mares' and cows' milk products collected from 12 different regions in Mongolia. Furthermore, we evaluated the fermentation properties of ten selected isolates of the predominant species, Streptococcus (S.) thermophiles, during the process of milk fermentation and subsequent storage of the resulting yoghurt at 4. Overall, 159 isolates were obtained from 188 samples using M17 agar. These isolates were presumed to be lactic acid bacteria based on their gram-positive and catalase-negative properties, and were identified to species level using 16S rRNA gene sequence analysis. These coccoid isolates were distributed in four genera and six species: Enterococcus (E.) durans, Enterococcus (E.) faecalis, Lactococcus (Lac.) subsp. lactis, Leuconostoc (Leuc.) lactis, Leuconostoc (Leuc.) mesenteroides. subsp. mesenteroides and S. thermophilus. Among these S. thermophilus was the most common species in most samples. From evaluation of the fermentation characteristics (viable counts, pH, titratable acidity [TA]) of ten selected S. thermophilus isolates we could identify four isolates (IMAU 20246, IMAU20764, IMAU20729 and IMAU20738) that were fast acid producers. IMAU20246 produced the highest concentrations of lactic acid and formic acid. These isolates have potential as starter cultures for yoghurt production.
Descriptors
Links
Book Title
Database
Publisher
Data Source
Authors
Ren,Y., Liu,W., Zhang,H.
Original/Translated Title
URL
Date of Electronic
20151031
PMCID
PMC4670899
Editors
Determination of polycyclic aromatic hydrocarbons in water samples using online microextraction by packed sorbent coupled with gas chromatography-mass spectrometry 2012 Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, China.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Talanta
Periodical, Abbrev.
Talanta
Pub Date Free Form
30-May
Volume
94
Issue
Start Page
152
Other Pages
157
Notes
CI: Copyright (c) 2012; JID: 2984816R; 0 (Polycyclic Hydrocarbons, Aromatic); 0 (Water Pollutants, Chemical); 2012/01/13 [received]; 2012/02/27 [revised]; 2012/03/04 [accepted]; 2012/03/10 [aheadofprint]; ppublish
Place of Publication
Netherlands
ISSN/ISBN
1873-3573; 0039-9140
Accession Number
PMID: 22608428
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.talanta.2012.03.010 [doi]
Output Language
Unknown(0)
PMID
22608428
Abstract
A fully automated microextraction by packed sorbents (MEPS) coupled with large volume injection gas chromatography-mass spectrometry (GC-MS) has been developed for the determination of eight polycyclic aromatic hydrocarbons (PAHs) in environmental water samples. Naphthalene (Nap), pyrene (Pyr), anthracene, acenaphthylene, phenanthrene, fluoranthene (Flr), fluorene and acenaphthene were the PAHs studied. The performance of the microextraction-GC-MS protocol was compared with solid phase extraction (SPE) and GC-MS analysis. Under optimized experimental conditions, the methods were linear for all analytes in the following ranges: 0.05-2.0 mug L(-1) (MEPS) and 0.25-10.0 mug L(-1) (SPE). The correlation coefficients (R(2)) were in the range 0.9965-0.9997 (MEPS) and 0.9978-0.9998 (SPE) for all the analytes. Limits of detection (LODs) for 2 mL samples (MEPS) ranged from 0.8 ng L(-1) to 8.2 ng L(-1). LODs for 50 mL samples (SPE) were between 4.8 ng L(-1) and 35.9 ng L(-1). The two methods were successfully applied to the determination of the 8 PAHs in environmental waters, with recoveries in the range of 70-117% (MEPS) and 72-134% (SPE) for a real spiked sample. The two sample preparation processes showed good repeatabilities with intra-day relative standard deviations below 14.0% (MEPS) and 14.6% (SPE). Nap, Flr and Pyr were found in a river water sample.
Descriptors
Links
Book Title
Database
Publisher
Elsevier B.V
Data Source
Authors
Fu,S., Fan,J., Hashi,Y., Chen,Z.
Original/Translated Title
URL
Date of Electronic
20120310
PMCID
Editors
A novel extraction technique based on carbon nanotubes reinforced hollow fiber solid/liquid microextraction for the measurement of piroxicam and diclofenac combined with high performance liquid chromatography 2012 Key Laboratory of Chemistry of Northwestern Plant Resources, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Talanta
Periodical, Abbrev.
Talanta
Pub Date Free Form
15-Oct
Volume
100
Issue
Start Page
153
Other Pages
161
Notes
LR: 20131121; CI: Copyright (c) 2012; JID: 2984816R; 0 (Nanotubes, Carbon); 0 (Salts); 0 (Solvents); 059QF0KO0R (Water); 13T4O6VMAM (Piroxicam); 144O8QL0L1 (Diclofenac); 2012/05/31 [received]; 2012/08/16 [revised]; 2012/08/27 [accepted]; 2012/09/03 [ahead
Place of Publication
Netherlands
ISSN/ISBN
1873-3573; 0039-9140
Accession Number
PMID: 23141323
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.talanta.2012.08.042 [doi]
Output Language
Unknown(0)
PMID
23141323
Abstract
A novel design of carbon nanotubes reinforced hollow fiber solid/liquid phase microextraction (CNTs-HF-SLPME) was developed to determine piroxicam and diclofenac in different real water samples. Functionalized multi-walled carbon nanotubes (MWCNTs) were held in the pores of hollow fiber with sol-gel technology. The pores and lumen of carbon nanotubes reinforced hollow fiber were subsequently filled with a muL volume of organic solvent (1-octanol), and then the whole assembly was used for the extraction of the target analytes in direct immersion sampling mode. The target analytes were extracted from the sample by two extractants, one of which is organic solvent placed inside the pores and lumen of hollow fiber and the other one is CNTs held in the pores of hollow fiber. After extraction, the analytes were desorbed in acetonitrile and analyzed using high performance liquid chromatography. This novel extraction mode showed more excellent extraction performance in comparison with conventional hollow fiber liquid microextraction (without adding CNTs) and carbon nanotubes reinforced hollow fiber solid microextraction (CNTs held in the pores of hollow fiber, but no organic solvents placed inside the lumen of hollow fiber) under the respective optimum conditions. This method provided 47- and 184-fold enrichment factors for piroxicam and diclofenac, respectively, good inter-fiber repeatability and batch-to-batch reproducibility. Linearity was observed in the range of 20-960 mug L(-1) for piroxicam, and 10-2560 mug L(-1) for diclofenac, with correlation coefficients of 0.9985 and 0.9989, respectively. The limits of detection were 4.58 mug L(-1) for piroxicam and 0.40 mug L(-1) for diclofenac.
Descriptors
Links
Book Title
Database
Publisher
Elsevier B.V
Data Source
Authors
Song,X.Y., Shi,Y.P., Chen,J.
Original/Translated Title
URL
Date of Electronic
20120903
PMCID
Editors
Polydopamine supported preparation method for solid-phase microextraction coatings on stainless steel wire 2011 Key Laboratory of Chemistry of Northwestern Plant Resources, CAS/Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, No. 18 Tianshui Road, Lanzhou, Gansu 730000, China.
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of chromatography.A
Periodical, Abbrev.
J.Chromatogr.A
Pub Date Free Form
10-Jun
Volume
1218
Issue
23
Start Page
3601
Other Pages
3607
Notes
LR: 20131121; CI: Copyright (c) 2011; JID: 9318488; 0 (Polycyclic Hydrocarbons, Aromatic); 0 (Soil); 0 (Water Pollutants, Chemical); 059QF0KO0R (Water); 12597-68-1 (Stainless Steel); 660YQ98I10 (Potassium Chloride); 91D9GV0Z28 (Durapatite); VTD58H1Z2X (Do
Place of Publication
Netherlands
ISSN/ISBN
1873-3778; 0021-9673
Accession Number
PMID: 21531422
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.chroma.2011.04.018 [doi]
Output Language
Unknown(0)
PMID
21531422
Abstract
In this paper, we introduced a novel and versatile route to prepare solid-phase microextraction coatings on the chemically inert stainless steel wire. Polydopamine films can be created on metallic substrates by an oxidant-induced polymerization and subsequently support various secondary reactions to prepare functional surfaces. In the present work, polydopamine-bioactivated stainless steel wire was successfully modified by nanostructured hydroxyapatite. Extraction performance of the fiber was assessed on several polycyclic aromatic hydrocarbons in water solutions. Extraction mechanism was suggested based on the correlation of partition coefficients and LogPs. Both aqueous and solid real life samples were used to test the reliability of the solid-phase microextraction-gas chromatography method; some analytes were detected and quantified.
Descriptors
Links
Book Title
Database
Publisher
Elsevier B.V
Data Source
Authors
Feng,J., Sun,M., Li,J., Xu,L., Liu,X., Jiang,S.
Original/Translated Title
URL
Date of Electronic
20110414
PMCID
Editors
Graphene oxide decorated with silver nanoparticles as a coating on a stainless-steel fiber for solid-phase microextraction 2015 Key Laboratory of Chemistry of Northwestern Plant Resources, CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, China.; Key Laboratory of Chemistry of Northwestern Pl
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of separation science
Periodical, Abbrev.
J.Sep.Sci.
Pub Date Free Form
Jul
Volume
38
Issue
14
Start Page
2439
Other Pages
2446
Notes
CI: (c) 2015; JID: 101088554; 0 (Oxides); 0 (Polycyclic Hydrocarbons, Aromatic); 12597-68-1 (Stainless Steel); 3M4G523W1G (Silver); 7782-42-5 (Graphite); OTO: NOTNLM; 2015/02/15 [received]; 2015/04/21 [revised]; 2015/04/21 [accepted]; 2015/06/03 [aheadofp
Place of Publication
Germany
ISSN/ISBN
1615-9314; 1615-9306
Accession Number
PMID: 25931422
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1002/jssc.201500308 [doi]
Output Language
Unknown(0)
PMID
25931422
Abstract
A novel graphene oxide decorated with silver nanoparticles coating on a stainless-steel fiber for solid-phase microextraction was prepared. Scanning electron microscopy and X-ray photoelectron spectroscopy were used to characterize the coating surface and showed that silver nanoparticles were dispersed on the wrinkled graphene oxide surface. Coupled to gas chromatography with flame ionization detection, the extraction abilities of the fiber for polycyclic aromatic hydrocarbons were examined in the headspace solid-phase microextraction mode. The extraction parameters including adsorption time, adsorption temperature, salt concentration, desorption time and desorption temperature were investigated. Under the optimized condition, wide linearity with low limits of detection from 2 to 10 ng/L was obtained. The relative standard deviations for single-fiber repeatability and fiber-to-fiber reproducibility were less than 10.6 and 17.5%, respectively. The enrichment factors were from 1712.5 to 4503.7, showing the fiber has good extraction abilities. Moreover, the fiber exhibited a good stability and could be reused for more than 120 times. The established method was also applied for determination of polycyclic aromatic hydrocarbons in two real water samples and the recoveries of analytes ranged from 84.4-116.3% with relative standard deviations less than 16.2%.
Descriptors
Links
Book Title
Database
Publisher
WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Data Source
Authors
Wang,L., Hou,X., Li,J., Liu,S., Guo,Y.
Original/Translated Title
URL
Date of Electronic
20150603
PMCID
Editors
Application of beta-cyclodextrin-modified, carbon nanotube-reinforced hollow fiber to solid-phase microextraction of plant hormones 2014 Key Laboratory of Chemistry of Northwestern Plant Resources of the CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China; University of Chinese Academy o
Source Type
Print(0)
Ref Type
Journal Article
Periodical, Full
Journal of chromatography.A
Periodical, Abbrev.
J.Chromatogr.A
Pub Date Free Form
29-Dec
Volume
1374
Issue
Start Page
23
Other Pages
30
Notes
LR: 20151119; CI: Copyright (c) 2014; JID: 9318488; 0 (Glycolates); 0 (Nanotubes, Carbon); 0 (Naphthaleneacetic Acids); 0 (Plant Growth Regulators); 0 (beta-Cyclodextrins); 33T7G7757C (1-naphthaleneacetic acid); 717GVR334R (2-naphthoxyacetic acid); JV039J
Place of Publication
Netherlands
ISSN/ISBN
1873-3778; 0021-9673
Accession Number
PMID: 25435464
Language
eng
SubFile
Journal Article; Research Support, Non-U.S. Gov't; IM
DOI
10.1016/j.chroma.2014.11.029 [doi]
Output Language
Unknown(0)
PMID
25435464
Abstract
A new, efficient, and environmental friendly solid-phase microextraction (SPME) medium based on beta-cyclodextrin (beta-CD)-modified carbon nanotubes (CNTs) and a hollow fiber (HF) was prepared. Functionalized beta-CD was covalently linked to the surface of the carboxylic CNTs and then the obtained nanocomposite was immobilized into the wall pores of HFs under ultrasonic-assisted effect. The scanning electron microscope was used to inspect surface characteristics of fibers, demonstrating the presence of nanocomposites in their wall pores. The reinforced HF was employed in SPME, and its extraction performance was evaluated by analyzing 1-naphthaleneacetic acid (NAA) and 2-naphthoxyacetic acid (2-NOA) in vegetables. Without any tedious clean-up procedure, analytes were extracted from the sample to the adsorbent and organic solvent immobilized in HFs and then desorbed in acetonitrile prior to chromatographic analysis. Under the optimized extraction conditions, the method provided 275- and 283-fold enrichment factors of NAA and 2-NOA, low limits of detection and quantification (at an ngg(-1) level), satisfactory spiked recoveries, good inter-fiber repeatability, and batch-to-batch reproducibility. The selectivity of the developed fiber was investigated to three structurally similar compounds and two reference compounds with recognition coefficients up to 3.18. The obtained results indicate that the newly developed fiber is a feasible, selective, green, and cost-effective microextraction medium and could be successfully applied for extraction and determination of naphthalene-derived plant hormones in complex matrices.
Descriptors
Links
Book Title
Database
Publisher
Elsevier B.V
Data Source
Authors
Song,X.Y., Ha,W., Chen,J., Shi,Y.P.
Original/Translated Title
URL
Date of Electronic
20141118
PMCID
Editors