WTS database

Title	Pub Year	Author	SearchLink Sort descending
Periodontal disease among indigenous people in the Amazon rain forest	2001	Oral Health Clinical Research Center, School of Dentistry, University of Minnesota, Minneapolis, USA. mrondero@uop.edu	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Journal of clinical periodontology Periodical, Abbrev. J.Clin.Periodontol. Pub Date Free Form Nov Volume 28 Issue 11 Start Page 995 Other Pages 1003 Notes LR: 20061115; JID: 0425123; ppublish Place of Publication Denmark ISSN/ISBN 0303-6979; 0303-6979 Accession Number PMID: 11686819 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; D; IM DOI 281102 [pii] Output Language Unknown(0) PMID 11686819 Abstract BACKGROUND: People are not all equally susceptible to periodontitis. To understand the epidemiology and natural history of this disease, it is important to study populations with varying genetic backgrounds and environmental exposures. AIM: Characterize the periodontal condition of a sample of indigenous adults in a remote region of the Amazon rain forest and determine the association of periodontal disease with various demographic, behavioral and environmental factors. METHODS: A cross-sectional evaluation of 244 subjects aged 20-70 years was conducted. Pocket depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), plaque and calculus were assessed for the Ramfjord index teeth. RESULTS: These people had high levels of plaque, calculus and BOP. The mean PD was rather shallow (2.45 mm in 20-29 year-olds to 2.73 mm in 50+ year-olds) and did not increase significantly with age. Mean CAL (0.57 mm in 20-29 year-olds and 2.26 mm in 50+ year-olds) and mean location of the free gingival margin in relation to the cemento-enamel junction changed significantly with age (p<0.0001). Multivariate analysis revealed that increasing age, bleeding on probing and calculus scores were positively associated with mean CAL (p<0.01). Sex, ethnicity, level of modern acculturation, use of coca or tobacco paste, frequency of dental visits and plaque were not associated with mean CAL. CONCLUSIONS: Periodontal disease in these people was mainly associated with gingival recession rather than deep pockets. Most people had clinical attachment loss but despite poor oral hygiene and extensive gingival inflammation, they did not have very severe periodontal destruction. Descriptors Acculturation, Adult, Age Factors, Aged, Colombia/epidemiology, Cross-Sectional Studies, Dental Calculus/epidemiology, Dental Care/statistics & numerical data, Dental Plaque/epidemiology, Disease Susceptibility, Environmental Exposure, Ethnic Groups/statistics & numerical data, Female, Gingival Hemorrhage/epidemiology, Gingival Recession/epidemiology, Gingivitis/epidemiology, Humans, Indians, South American/statistics & numerical data, Linear Models, Male, Middle Aged, Multivariate Analysis, Oral Hygiene, Periodontal Attachment Loss/epidemiology, Periodontal Diseases/epidemiology/genetics, Periodontal Pocket/epidemiology, Regression Analysis, Sex Factors, Smoking/epidemiology, Tobacco Links Book Title Database Publisher Data Source Authors Ronderos,M., Pihlstrom,B. L., Hodges,J. S. Original/Translated Title URL Date of Electronic PMCID Editors
AAPI youth tobacco use: a comparative analysis of current cigarette use data from the Florida, Texas, and National Youth Tobacco Surveys	2001	Division of Health Care Management, School of Allied Health Sciences, 223 Ware Rhaney Bldg., Florida A&M University, Tallahassee, FL 32307, USA. kershawj@hotmail.com	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Asian American and Pacific Islander journal of health Periodical, Abbrev. Asian Am.Pac.Isl.J.Health. Pub Date Free Form Winter-Spring Volume 9 Issue 1 Start Page 25 Other Pages 33 Notes LR: 20151119; JID: 9417466; ppublish Place of Publication United States ISSN/ISBN 1072-0367; 1072-0367 Accession Number PMID: 11720411 Language eng SubFile Comparative Study; Journal Article; IM DOI Output Language Unknown(0) PMID 11720411 Abstract OBJECTIVES: The objectives of this study were to compile data on Asian American and Pacific Islander (AAPI) youth tobacco use in Florida and conduct comparisons with state and national data. This research will contribute to reducing the gap in information regarding current smoking prevalence among AAPI youth in Florida and provide direct comparisons with another state (Texas) and National Youth Tobacco Survey (NYTS) data on AAPI youth. METHODS: Current cigarette use data from the Florida Youth Tobacco Surveys conducted in 1998, 1999, and 2000 were examined for trends in AAPI and state prevalence rates. AAPI data from Florida's baseline 1998 youth tobacco survey were compared to Texas data after applying a common set of data preparation edits. AAPI data from the NYTS were also compared to Florida's AAPI youth population. FINDINGS: Current cigarette use for AAPI students in Florida was generally below the overall prevalence rates among Florida's public middle or high school students. In 1998, current smoking prevalence among Texas AAPI middle and high school students was 18.7% compared to 19.4% among Florida students. Among high school students, the NYTS found that 21.2% of AAPI students were current cigarette smokers nationally in comparison to 21.7% of AAPI high school youth in Florida. In middle school, the current smoking prevalence among AAPI students was 5.5% in the NYTS as compared to 9.4% in Florida. The NYTS data in particular highlight the magnitude of the increasing trend of cigarette smoking among AAPI youth as they progress through the high school grades. CONCLUSIONS: Of all the racial/ethnic groups in Florida, only AAPIs did not have a significant decline in current cigarette use. While the Florida Tobacco Pilot Program has implemented many worthwhile initiatives, the anti-tobacco interventions do not appear to have exerted a noticeable effect on AAPI youth. Descriptors Adolescent, Asian Americans/statistics & numerical data, Child, Female, Florida/epidemiology, Humans, Male, Population Surveillance, Prevalence, Smoking/ethnology/trends, Surveys and Questionnaires, Texas/epidemiology, United States/epidemiology Links Book Title Database Publisher Data Source Authors Kershaw,J. M. Original/Translated Title URL Date of Electronic PMCID Editors
A review of the relationship between patent foramen ovale and type II decompression sickness	2001	Division of Occupational Medicine, University of Toronto, Canada. joan.saary@utoronto.ca	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Aviation, Space, and Environmental Medicine Periodical, Abbrev. Aviat.Space Environ.Med. Pub Date Free Form Dec Volume 72 Issue 12 Start Page 1113 Other Pages 1120 Notes LR: 20051116; JID: 7501714; RF: 82; ppublish Place of Publication United States ISSN/ISBN 0095-6562; 0095-6562 Accession Number PMID: 11763113 Language eng SubFile Journal Article; Review; IM; S DOI Output Language Unknown(0) PMID 11763113 Abstract The significance of patent foramen ovale (PFO) in the pathophysiology of Type II decompression sickness (DCS) remains controversial. PFOs are common, occurring in approximately one quarter of the normal population, thus making right-to-left shunting of venous gas emboli (VGE) a theoretical concern in both hyper- and hypobaric situations. Despite this high prevalence of PFO in the general population, and the relatively common occurrence of venous gas bubbles in diving and altitude exposures, the incidence of Type II DCS in diving or with altitude is remarkably low. Although the literature supports a relationship between the presence and size of PFO and cryptogenic stroke, and an increased relative risk of Type II DCS with a PFO in divers, the absolute increase in risk accrued is small. Hence, the value of screening is also controversial. This paper presents a summary of the literature on PFOs and DCS in animals, and in human altitude and diving, focusing on the latter; as well the analogous literature on cryptogenic stroke. The results of an examination of the literature on detection of, screening for, and treatment of PFOs is also presented. Descriptors Altitude, Animals, Brain/pathology, Decompression Sickness/etiology/physiopathology, Diving, Echoencephalography, Heart Septal Defects, Atrial/complications/therapy, Humans, Magnetic Resonance Imaging, Military Personnel, Ultrasonography, Doppler Links Book Title Database Publisher Data Source Authors Saary,M. J., Gray,G. W. Original/Translated Title URL Date of Electronic PMCID Editors
Quantitative detection of bisphenol A and bisphenol A diglycidyl ether metabolites in human plasma by liquid chromatography-electrospray mass spectrometry	2001	Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Journal of chromatography.B, Biomedical sciences and applications Periodical, Abbrev. J.Chromatogr.B Biomed.Sci.Appl. Pub Date Free Form 25-Dec Volume 765 Issue 2 Start Page 121 Other Pages 126 Notes LR: 20131121; JID: 9714109; 0 (Benzhydryl Compounds); 0 (Epoxy Compounds); 0 (Phenols); F3XRM1NX4H (2,2-bis(4-glycidyloxyphenyl)propane); MLT3645I99 (bisphenol A); ppublish Place of Publication Netherlands ISSN/ISBN 1387-2273; 1387-2273 Accession Number PMID: 11767304 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI Output Language Unknown(0) PMID 11767304 Abstract Due to the ubiquity of epoxy resin compounds and their potential role in increasing the risk for reproductive dysfunction and cancer, the need for an assessment of human exposure is urgent. Therefore, we developed a method for measuring bisphenol A (BPA) and bisphenol A diglycidyl ether (BADGE) metabolites in human blood samples using high-performance liquid chromatography-electrospray ionization mass spectrometry (LC-MS). Human blood samples were processed using enzymatic deconjugation of the glucuronides followed by a novel sample preparation procedure using a solid-phase-cartridge column. This selective analytical method permits rapid detection of the metabolites, free BPA and a hydrolysis product of BADGE (BADGE-40H) with detection limits in the low nanogram per milliliter range (0.1 ng ml(-1) of BPA and 0.5 ng ml(-1) of BADGE-40H). The sample extraction was achieved by Oasis HLB column on gradient elution. The recoveries of BPA and BADGE-40H added to human plasma samples were above 70.0% with a standard deviation of less than 5.0%. This selective, sensitive and accurate method will assist in elucidating potential associations between human exposure to epoxy-based compounds and adverse health effects. Descriptors Benzhydryl Compounds, Chromatography, Liquid/methods, Epoxy Compounds/analysis, Humans, Phenols/blood, Spectrometry, Mass, Electrospray Ionization/methods Links Book Title Database Publisher Data Source Authors Inoue,K., Yamaguchi,A., Wada,M., Yoshimura,Y., Makino,T., Nakazaw,H. Original/Translated Title URL Date of Electronic PMCID Editors
Stroke in a scuba diver with patent foramen ovale	2002	Dipartimento di Scienze Neurologiche, Universita degli Studi La Sapienza, viale dell'Universita no. 30, 00185 Rome, Italy. carla.buttinelli@uniroma1.it	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full European journal of neurology Periodical, Abbrev. Eur.J.Neurol. Pub Date Free Form Jan Volume 9 Issue 1 Start Page 89 Other Pages 91 Notes LR: 20151113; JID: 9506311; ppublish Place of Publication England ISSN/ISBN 1351-5101; 1351-5101 Accession Number PMID: 11784382 Language eng SubFile Case Reports; Journal Article; IM DOI 347 [pii] Output Language Unknown(0) PMID 11784382 Abstract Patent foramen ovale (PFO) is a frequent condition which carries a significant risk for stroke when associated with deep venous thrombosis and primary or secondary coagulation abnormalities. Here, we describe a patient in which scuba diving is thought to be associated with stroke in a subject with an otherwise clinically silent PFO. During a rapid ascent a 43-year-old-scuba diver reported weakness and paresthesias in the right arm which lasted about 10 min. He presented similar symptoms 2 days later 1 h after diving, and a third time on his flight back home. The MRI showed multiple hyperintense areas on T2-weighted images in the white matter. Transoesophageal echocardiography (TEE) showed a PFO, whilst all haematological and haemocoagulation tests were negative. Scuba diving may constitute a patho-physiological condition in the presence of PFO as breath-holding promotes right-to-left shunt and arterialization of venous bubbles. Descriptors Adult, Brain/pathology, Diving, Electroencephalography, Electromyography, Heart Septal Defects, Atrial/complications/pathology, Humans, Magnetic Resonance Imaging, Male, Stroke/etiology/pathology/radiography, Ultrasonography, Doppler, Transcranial Links Book Title Database Publisher Data Source Authors Buttinelli,C., Beccia,M., Argentino,C. Original/Translated Title URL Date of Electronic PMCID Editors
Ethnic differences in cigarette smoking among adolescents: a comparison of Jews and Arabs in Jerusalem	2001	Pediatric Respiratory Medicine Unit, Department of Pediatrics, Shaare Zedek Medical Center (affiliated to Hebrew University-Hadassah Medical School), Jerusalem, Israel.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full The Israel Medical Association journal : IMAJ Periodical, Abbrev. Isr.Med.Assoc.J. Pub Date Free Form Jul Volume 3 Issue 7 Start Page 504 Other Pages 507 Notes LR: 20061115; JID: 100930740; ppublish Place of Publication Israel ISSN/ISBN 1565-1088 Accession Number PMID: 11791417 Language eng SubFile Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; IM DOI Output Language Unknown(0) PMID 11791417 Abstract BACKGROUND: Prevention of cigarette smoking is an important issue in public health policy. Since most adult smokers began smoking in childhood, understanding behavorial factors associated with smoking initiation would contribute to smoking initiation programs. Health-related behavior may vary between different ethnic groups. OBJECTIVES: To determine the prevalence of smoking among Jewish and Arab adolescents in Jerusalem, and whether there are differences in smoking initiation between the two ethnic groups. METHODS: We carried out a cross-sectional survey of all students in the 6th to 11th grades (age range 11-17 years) of a Jewish school and an Arab school in the Jerusalem area, using an anonymous self-completion questionnaire. A total of 791 questionnaires was analyzed, 479 from the Jewish students and 312 from the Arab students. RESULTS: The lowest prevalence of smoking was found among Arab female students and the highest among Jewish female students (9% vs. 41%, P < 0.001). The prevalence of smoking among Jewish and Arab males was similar. More Arab female students smoked than their mothers. Peer pressure seemed to be a more important factor among Jewish students. CONCLUSIONS: This study demonstrated the presence of ethnic differences in smoking prevalence and the reasons for smoking among adolescents in Israel. These results suggest the need for specific smoking prevention policies for different ethnic groups. Descriptors Adolescent, Adolescent Behavior/ethnology/psychology, Age Distribution, Arabs/psychology/statistics & numerical data, Child, Cross-Sectional Studies, Female, Health Behavior/ethnology, Health Surveys, Humans, Israel/epidemiology, Jews/psychology/statistics & numerical data, Male, Sex Distribution, Smoking/ethnology/psychology, Smoking Cessation/ethnology/psychology Links Book Title Database Publisher Data Source Authors Meijer,B., Branski,D., Kerem,E. Original/Translated Title URL Date of Electronic PMCID Editors
ER-YAG laser pretreatment effect on in vitro secondary caries formation around composite restorations	2001	Departamento de Materiales Dentales, Escuela de Odontologia, Universidad de Granada, Spain.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full American Journal of Dentistry Periodical, Abbrev. Am.J.Dent. Pub Date Free Form Feb Volume 14 Issue 1 Start Page 46 Other Pages 49 Notes LR: 20151119; JID: 8806701; 0 (Acetates); 0 (Aluminum Silicates); 0 (Composite Resins); 0 (Phosphoric Acids); 0 (Resin Cements); 0 (Z100 composite resin); 58784XQC3Y (Yttrium); 7631-86-9 (Silicon Dioxide); 77B218D3YE (Erbium); 90881-69-9 (Scotchbond); C6V Place of Publication United States ISSN/ISBN 0894-8275; 0894-8275 Accession Number PMID: 11806480 Language eng SubFile Clinical Trial; Comparative Study; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't; D DOI Output Language Unknown(0) PMID 11806480 Abstract PURPOSE: This in vitro study determined if Er-YAG laser used in instead of acid-etching influenced artificial secondary caries formation in enamel and root surfaces. MATERIALS AND METHODS: Class V cavities were prepared in buccal and lingual surfaces of 10 extracted caries-free molars, with cervical margins in the root surface and occlusal margins in enamel. The specimens were randomly assigned to 2 groups: Group 1: Enamel and dentin etched with 35% phosphoric acid gel (Scotchbond 15s, rinse 10s; n=5 teeth with 2 cavities per specimen, 10 occlusal and 10 root surface margins at caries risk). Group 2: Enamel and dentin surfaces conditioned using a pulsed Er-YAG laser (KAVO) with 2.94 microm wavelength, 250 micros pulse duration, 300 mJ for enamel and 250 mJ for root surface pulse energy, 2 Hz repetition rate, and water cooling (n=5 teeth with 2 cavities per specimen, 10 occlusal and 10 root surface margins at caries risk). The cavity preparations were restored with a wet-bonding technique (Scotchbond 1 adhesive system) and a hybrid resin, light-cured composite (Z100, A3 shade), according to the manufacturer's instructions. Acid-resistant varnish was applied leaving the restoration and a 1 mm rim of adjacent surface enamel and root surface exposed. The specimens were thermocycled (5-50 degrees C, 500 cycles, dwell time 30s). Following artificial caries formation (2.2 mM calcium, 2.2 mM phosphate, 50 mM acetic acid, 5.0 mg/L fluoride, pH 4.25, 10 days), longitudinal sections (3/tooth, 30 occlusal and cervical caries risk sites per group) were taken for polarized light microscopic examination (water imbibition). Primary surface lesion depth and wall lesion frequency was determined and compared between groups (Student's t-test). RESULTS: Er-YAG laser irradiation resulted in a 56% reduction in primary enamel surface lesion depth (116 microm mean depth) when compared with the acid-etched group (263 microm mean depth), and a 39% decrease in root surface lesion depth (194 microm mean depth) compared with that (316 microm mean depth) for acid-etching pretreatment (P0.05) between treatment groups. Descriptors Acetates, Acid Etching, Dental, Aluminum Silicates, Composite Resins/chemistry, Dental Caries/pathology/prevention & control, Dental Caries Susceptibility, Dental Cavity Preparation/classification, Dental Enamel/radiation effects/ultrastructure, Dental Restoration, Permanent, Dentin/radiation effects/ultrastructure, Erbium, Humans, Hydrogen-Ion Concentration, Low-Level Light Therapy, Microscopy, Polarization, Molar, Phosphoric Acids/administration & dosage, Recurrence, Resin Cements/chemistry, Silicon Dioxide, Statistics as Topic, Thermodynamics, Tooth Demineralization/pathology/prevention & control, Tooth Root/radiation effects/ultrastructure, Yttrium, Zirconium Links Book Title Database Publisher Data Source Authors Ceballos,L., Toledano,M., Osorio,R., Garcia-Godoy,F., Flaitz,C., Hicks,J. Original/Translated Title URL Date of Electronic PMCID Editors
Helicobacter pylori infection, not gastroesophageal reflux, is the major cause of inflammation and intestinal metaplasia of gastric cardiac mucosa	2002	Center for Swallowing and Esophageal Disorders and Department of Anatomic Pathology, Cleveland Clinic Foundation, Ohio 44195, USA.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full The American Journal of Gastroenterology Periodical, Abbrev. Am.J.Gastroenterol. Pub Date Free Form Feb Volume 97 Issue 2 Start Page 302 Other Pages 311 Notes LR: 20061115; JID: 0421030; ppublish Place of Publication United States ISSN/ISBN 0002-9270; 0002-9270 Accession Number PMID: 11866266 Language eng SubFile Comparative Study; Journal Article; IM DOI 10.1111/j.1572-0241.2002.05462.x [doi] Output Language Unknown(0) PMID 11866266 Abstract OBJECTIVE: The etiology of inflammation below the normal Z-line is an area of intense debate. Some suggest this is the earliest change of chronic gastroesophageal reflux disease (GERD), whereas others indict Helicobacter pylori (H. pylori) as the main cause. The aim of this study was to evaluate the relationship among inflammation of gastric cardiac mucosa (carditis), H. pylori infection, and intestinal metaplasia in patients with GERD and Barrett's esophagus compared with age-matched controls. METHODS: Patients with GERD and Barrett's esophagus were compared with controls undergoing endoscopy for a variety of other conditions. Endoscopic biopsy specimens from the gastric cardia (obtained on retroflexed view), fundus, and antrum were evaluated for inflammation, H. pylori infection, and intestinal metaplasia. RESULTS: The prevalence of H. pylori infection did not significantly differ among the study populations: controls (42%), GERD (33%), and Barrett's esophagus (27%) (p = 0.20). However, the prevalence of carditis significantly decreased from the control group (30%) to those with GERD (23%) and Barrett's esophagus (11%) (p = 0.03). Overall, 42 of 51 (82%) patients with carditis had H. pylori; all had pangastritis. The prevalence of cardia intestinal metaplasia also significantly decreased from the control group (15%) to those with GERD (4%) and Barrett's esophagus (0%) (p = 0.003). Of 13 patients with cardia intestinal metaplasia, 12 had carditis, 10 had H. pylori infection, and seven had intestinal metaplasia elsewhere in the stomach. CONCLUSIONS: Inflammation of gastric cardiac mucosa decreases in prevalence from controls to patients with GERD and Barrett's esophagus and correlates strongly with H. pylori infection. Cardia intestinal metaplasia is associated with H. pylori-related cardiac inflammation and intestinal metaplasia elsewhere in the stomach. Descriptors Adult, Age Distribution, Aged, Aged, 80 and over, Analysis of Variance, Barrett Esophagus/complications/pathology, Biopsy, Needle, Cardia/pathology, Case-Control Studies, Cohort Studies, Esophagoscopy/methods, Female, Gastric Mucosa/pathology, Gastritis/epidemiology/etiology/pathology, Gastroesophageal Reflux/complications/pathology, Gastroscopy/methods, Helicobacter Infections/complications/pathology, Helicobacter pylori/isolation & purification, Humans, Intestinal Mucosa/pathology, Male, Middle Aged, Prevalence, Prognosis, Risk Assessment, Risk Factors, Sex Distribution Links Book Title Database Publisher Data Source Authors Goldblum,J. R., Richter,J. E., Vaezi,M., Falk,G. W., Rice,T. W., Peek,R. M. Original/Translated Title URL Date of Electronic PMCID Editors
The potential of a polyphasic PCR-dGGE approach in evaluating microbial diversity of natural whey cultures for water-buffalo Mozzarella cheese production: bias of culture-dependent and culture-independent analyses	2001	Dipartimento di Scienza degli Alimenti, Alimentare e Ambientale e di Igiene, Stazione di Microbiologia Industriale, Universita degli Studi di Napoli Federico II, Portici, Italy.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full Systematic and applied microbiology Periodical, Abbrev. Syst.Appl.Microbiol. Pub Date Free Form Dec Volume 24 Issue 4 Start Page 610 Other Pages 617 Notes LR: 20061115; JID: 8306133; 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); ppublish Place of Publication Germany ISSN/ISBN 0723-2020; 0723-2020 Accession Number PMID: 11876368 Language eng SubFile Journal Article; Research Support, Non-U.S. Gov't; IM DOI S0723-2020(04)70075-2 [pii] Output Language Unknown(0) PMID 11876368 Abstract A polyphasic PCR-DGGE approach was used to describe the microbial population occurring in natural whey cultures (NWCs) for water-buffalo Mozzarella cheese production. Total microbial community was assessed without cultivation by analyzing DNA directly extracted from the original samples of NWC. In addition, DNA extracted from bulks of cells formed by harvesting colonies from the serial dilution agar plates of a variety of culture media was used to profile the "cultivable" community. The 16S rDNA V3 region was amplified using DNA from NWC as well as DNA from bulks as templates and the amplicons were separated by DGGE. The microbial entities occurring in NWCs were identified by partial 16S rDNA sequencing of DGGE bands: four lactic acid bacteria (LAB) closest relative of Streptococcus thermophilus, Lactococcus lactis, Lactobacillus delbrueckii and Lactobacillus crispatus were revealed by the analysis of DNA directly extracted from NWC while two other LAB, Lactobacillus fermentum and Enterococcus faecalis, were identified by analyzing DNA from the cultivable community. The developed PCR-DGGE analysis of the "cultivable" community showed good potential in evaluating microbial diversity of a dairy environment: it usefully highlighted the bias introduced by selective amplification when compared to the analysis of the total community from NWC and allowed suitability of media and growth conditions to be evaluated. Moreover, it could be used to complete the culture independent study of microbial diversity to give information on concentration ratios among species occurring in a particular environment and can be proposed for rapid identification of dominant microorganisms in alternative to traditional tools. Descriptors Animals, Base Sequence, Buffaloes, Cheese/microbiology, Colony Count, Microbial, DNA, Bacterial/chemistry/genetics, Electrophoresis, Polyacrylamide Gel, Enterococcus faecalis/genetics/isolation & purification, Food Microbiology, Italy, Lactobacillus/genetics/isolation & purification, Molecular Sequence Data, Polymerase Chain Reaction, RNA, Ribosomal, 16S/chemistry/genetics, Sequence Analysis, DNA, Streptococcus/genetics/isolation & purification Links Book Title Database Publisher Data Source Authors Ercolini,D., Moschetti,G., Blaiotta,G., Coppola,S. Original/Translated Title URL Date of Electronic PMCID Editors
Legionella contamination of hospital water supplies: monitoring of private healthcare facilities in Bologna, Italy	2002	Department of Medicine and Public Health, University of Bologna, Italy.	Read more...
Source Type Print(0) Ref Type Journal Article Periodical, Full The Journal of hospital infection Periodical, Abbrev. J.Hosp.Infect. Pub Date Free Form Mar Volume 50 Issue 3 Start Page 220 Other Pages 223 Notes LR: 20081121; CI: Copyright 2002; JID: 8007166; ppublish Place of Publication England ISSN/ISBN 0195-6701; 0195-6701 Accession Number PMID: 11886199 Language eng SubFile Journal Article; IM DOI 10.1053/jhin.2002.1180 [doi] Output Language Unknown(0) PMID 11886199 Abstract The hot water supplies of 11 private healthcare facilities in the city of Bologna, Italy, were monitored for the presence of Legionella spp. Four samplings were made in each establishment over a period of one year and in total 121 samples were collected from distribution points situated near the water-boiler and inside the wards (taps and showers). Legionellae were recovered from all the water supplies in question: Legionella spp. in 86.8% of samples, L. pneumophila in 82.6% of samples. L. pneumophila was found in all the water supplies at levels averaging above 10(4)cfu/L in five health facilities and reaching a maximum concentration of 10(7)cfu/L. The only parameter to have affected the presence of legionellae was the water temperature, which was seen to be inversely correlated to the concentration of Legionella spp. Despite the high levels of contamination from L. pneumophila, no cases of nosocomial Legionnaires' disease were reported during the period of the study. Descriptors Cross Infection/epidemiology, Hospitals, Private, Hot Temperature, Humans, Italy, Legionella/isolation & purification, Legionnaires' Disease/epidemiology, Water Microbiology, Water Supply Links Book Title Database Publisher The Hospital Infection Society Data Source Authors Legnani,P. P., Leoni,E., Corradini,N. Original/Translated Title URL Date of Electronic PMCID Editors

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