Molecular characterization of extended-spectrum beta-lactamase-producing Escherichia coli isolates from chickens in Henan Province, China

Journal Article

Print(0)

Journal of medical microbiology

J.Med.Microbiol.

Nov

Pt 11

1449

1453

GENBANK/FJ405211; JID: 0224131; 0 (DNA, Bacterial); EC 3.5.2.- (beta-lactamase TEM-3); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (beta-lactamase TEM-1); 2009/07/02 [aheadofprint]; ppublish

England

1473-5644; 0022-2615

PMID: 19574412

eng

Journal Article; Research Support, Non-U.S. Gov't; IM

10.1099/jmm.0.012229-0 [doi]

Unknown(0)

19574412

Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli has spread rapidly worldwide and poses a serious threat to human and animal health. This study collected 51 non-replicate E. coli isolates from 14 different chicken farms in Henan Province in China from December 2007 to August 2008. The prevalence of ESBL-producing E. coli, molecular characterization of the ESBL-related bla genes, including bla(TEM), bla(SHV) and bla(CTX-M), and the susceptibilities of these bacteria to various antimicrobial agents were determined. Thirty-one of the 51 isolates were positive for an ESBL phenotype and 29 of these isolates carried one or more bla genes. Twenty-two isolates harboured bla(TEM) genes and 15 isolates carried bla(CTX-M) genes (one CTX-M-14, three CTX-M-24 and 11 CTX-M-65). One isolate carried bla(TEM)(-57); the remaining bla(TEM) isolates carried bla(TEM-1) with one silent nucleotide base variation (T18C). We believe that this is the first study to report TEM-57 in E. coli isolates. All isolates harbouring bla(CTX-M-24) and bla(CTX-M-14) and five of the bla(CTX-M-65) isolates also harboured the bla(TEM-1) gene. To our knowledge, this study is the first to describe detection of CTX-M-65-producing E. coli isolated from chickens. None of the isolates contained the bla(SHV) gene. Conjugation experiments demonstrated that bla(CTX-M) and bla(TEM) genes could be transferred to E. coli DH5 alpha. The results indicate that ESBL frequency has reached an alarming level in chicken isolates in China, with TEM-1 and CTX-M-65 enzymes being the two predominant beta-lactamases detected.

Animals, Chickens/microbiology, China, DNA, Bacterial/analysis, Escherichia coli/enzymology/isolation & purification, Escherichia coli Infections/epidemiology/microbiology/veterinary, Molecular Sequence Data, Polymerase Chain Reaction, Poultry Diseases/epidemiology/microbiology, Sequence Analysis, DNA, beta-Lactamases/biosynthesis/classification/genetics

Yuan,L., Liu,J. H., Hu,G. Z., Pan,Y. S., Liu,Z. M., Mo,J., Wei,Y. J.

Department of Pharmacology and Toxicology, College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou 450002, PR China.

20090702

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=19574412

2009