Journal Article

Print(0)

International journal of antimicrobial agents

Int.J.Antimicrob.Agents

May

29

5

576

581

JID: 9111860; 0 (Anti-Bacterial Agents); EC 3.5.2.6 (beta-Lactamases); 2006/09/20 [received]; 2006/12/22 [revised]; 2006/12/22 [accepted]; 2007/02/20 [aheadofprint]; ppublish

Netherlands

0924-8579; 0924-8579

PMID: 17314033

eng

Journal Article; Research Support, Non-U.S. Gov't; IM

S0924-8579(07)00038-6 [pii]

Unknown(0)

17314033

The objective of this study was to characterise the beta-lactamase genes of cephalosporin-resistant Escherichia coli isolated from farm animals in Guangdong Province of China. Of 592 E. coli isolates recovered from farm animals from 2003-2005, 50 (8.4%) showed cephalosporin resistance. Polymerase chain reaction and sequencing analysis showed that 14 isolates (2.4%) from chickens, ducks, pigs and partridges were positive for the bla(CTX-M) gene (10 for bla(CTX-M-14) and 4 for bla(CTX-M-27)). CMY-2 was detected for the first time in mainland China in six E. coli isolates (1.0%) from chickens and goose. Except for one isolate, bla(CTX-M)- and bla(CMY-2)-containing isolates also harboured the bla(TEM-1b) gene. Conjugation experiments demonstrated that the bla(CTX-M) and bla(TEM) genes could be transferred to E. coli DH5alpha. Pulsed-field gel electrophoresis (PFGE) showed that the 14 CTX-M-producing isolates belonged to 12 different types. Two isolates (one from a chicken, the other from a pig) containing CTX-M-14 showed indistinguishable PFGE patterns, indicating clonal dissemination of this strain among animals from different farms. This study describes for the first time the emergence of CTX-M- and CMY-2-producing E. coli among farm animals in China, with the CTX-M-9 group being the predominant extended-spectrum beta-lactamase detected.

Animals, Animals, Domestic/microbiology, Anti-Bacterial Agents/pharmacology, Cephalosporin Resistance, Chickens, China, Cloning, Molecular, Drug Resistance, Bacterial, Ducks, Electrophoresis, Gel, Pulsed-Field, Escherichia coli/drug effects/enzymology, Feces/microbiology, Microbial Sensitivity Tests, Swine, beta-Lactamases/chemistry/genetics/isolation & purification

Liu,J. H., Wei,S. Y., Ma,J. Y., Zeng,Z. L., Lu,D. H., Yang,G. X., Chen,Z. L.

College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China.

20070220

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=17314033

2007