Bacterial biodiversity of traditional Zabady fermented milk

Journal Article

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International journal of food microbiology

Int.J.Food Microbiol.

10-Feb

121

295

301

JID: 8412849; 0 (DNA, Bacterial); 2006/11/09 [received]; 2007/04/11 [revised]; 2007/11/06 [accepted]; 2007/11/17 [aheadofprint]; ppublish

Netherlands

0168-1605; 0168-1605

PMID: 18077039

eng

Journal Article; IM

S0168-1605(07)00587-9 [pii]

Unknown(0)

18077039

The aim of this work was to identify the bacterial biodiversity of traditional Zabady fermented milk using PCR-temporal temperature gel electrophoresis (PCR-TTGE) and PCR-denaturing gradient gel electrophoresis (PCR-DGGE). Most of the identified bacterial species in Zabady samples belonged to lactic acid bacteria (LAB), e.g., Streptococcus thermophilus, Lactococcus garvieae, Lactococcus raffinolactis, Lactococcus lactis, Leuconostoc citreum, Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus johnsonii. Using the culture-dependent and independent methods, the streptococcal and lactococcal groups appeared to be the major bacterial species in Zabady fermented milk, whereas the lactobacilli were the minor ones. The main dominant species was St. thermophilus followed by Lc. garvieae. Other molecular tools, e.g., species-specific PCR assay and cloning and sequencing strategy were used to confirm the TTGE and DGGE results. Lc. garvieae, Lc. raffinolactis, Ln. citreum, and Lb. johnsonii were identified for the first time in this type of Egyptian fermented milk.

Biodiversity, Colony Count, Microbial, Cultured Milk Products/microbiology, DNA, Bacterial/analysis, Egypt, Electrophoresis, Gel, Pulsed-Field/methods, Food Microbiology, Lactobacillus/classification/isolation & purification, Lactococcus/classification/isolation & purification, Leuconostoc/classification/isolation & purification, Phylogeny, Polymerase Chain Reaction/methods, Species Specificity, Streptococcus/classification/isolation & purification

El-Baradei,G., Delacroix-Buchet,A., Ogier,J. C.

Department of Dairy Science and Technology, Faculty of Agriculture, Alexandria University, Aflaton street, El-Shatby, Alexandria, Egypt. elbaradeig@yahoo.fr

20071117

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2008