Sensitive determination of 2,4,6-trichloroanisole in water samples by ultrasound assisted emulsification microextraction prior to gas chromatography-tandem mass spectrometry analysis

Journal Article

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Talanta

15-Jun

5-Apr

1536

1541

LR: 20131121; JID: 2984816R; 0 (2,4,6-trichloroanisole); 0 (Anisoles); 0 (Emulsifying Agents); 0 (Phosphates); 0 (Water Pollutants, Chemical); 7V31YC746X (Chloroform); 2009/10/27 [received]; 2010/02/24 [revised]; 2010/02/25 [accepted]; 2010/03/06 [aheadof

England

1873-3573; 0039-9140

PMID: 20441935

eng

Journal Article; Research Support, Non-U.S. Gov't; IM

10.1016/j.talanta.2010.02.065 [doi]

Unknown(0)

20441935

A novel application of an ultrasound assisted emulsification microextraction (USAEME) technique is proposed for the extraction and preconcentration of 2,4,6-trichloroanisole (2,4,6-TCA) from water samples prior to its determination by gas chromatography-tandem mass spectrometry (GC-MS/MS). USAEME employs a non-polar high-density solvent (extractant solvent), which forms an oil-in-water emulsion (O/W) in the aqueous sample bulk assisted by ultrasonic radiation. Several factors including, solvent type and volume, extraction time, extraction temperature, shaking mode and matrix modifiers were studied and optimized over the relative recovery of the target analyte. An aliquot of 5mL water sample was conditioned by adding 150microL 6.15molL(-1) sodium chloride and 300microL 0.05molL(-1) phosphate buffer (pH 6), and finally extracted with 40microL chloroform by using USAEME technique. Under the optimal experimental conditions 2,4,6-TCA was quantitatively extracted achieving an enrichment factor (EF) of 555. The detection limit (LOD), calculated as three times the signal-to-noise ratio (S/N), was 0.2ngL(-1) and the RSD was 6.3% (n=5) when 1ngL(-1) 2,4,6-TCA standard mixture was analyzed. The coefficients of estimation of the calibration curves obtained following the proposed methodology was >or=0.997 and the linear working range was 1-5000ngL(-1). Finally, the proposed technique was successfully applied for extraction and determination of the 2,4,6-TCA in water samples. Recovery studies lead values >or=94%, which showed a successfully robustness of the analytical methodology for determination of nanogram per liter of 2,4,6-TCA in water samples.

Analytic Sample Preparation Methods/methods, Anisoles/analysis, Calibration, Chloroform/chemistry, Chromatography, Gas/methods, Emulsifying Agents/chemistry, Mass Spectrometry/methods, Phosphates/chemistry, Reproducibility of Results, Sensitivity and Specificity, Tandem Mass Spectrometry/methods, Temperature, Ultrasonics, Water Pollutants, Chemical/analysis, Water Purification

Fontana,A. R., Altamirano,J. C.

Grupo de Investigacion y Desarrollo en Quimica Analitica (QUIANID) (LISAMEN, CCT CONICET - Mendoza), Av. Ruiz Leal S/N, Parque General San Martin, Mendoza, Argentina.

20100306

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=20441935

2010