Journal Article

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The Journal of veterinary medical science / the Japanese Society of Veterinary Science

J.Vet.Med.Sci.

75

8

1009

1015

JID: 9105360; 0 (DNA Primers); 0 (Escherichia coli Proteins); 9M416Z9QNR (Ceftazidime); EC 3.5.2.- (CTX-M-9 protein, E coli); EC 3.5.2.- (beta-lactamase CMY-2); EC 3.5.2.6 (beta-Lactamases); N2GI8B1GK7 (Cefotaxime); 2013/03/15 [aheadofprint]; ppublish

Japan

1347-7439; 0916-7250

PMID: 23503164

eng

Journal Article; IM

DN/JST.JSTAGE/jvms/12-0453 [pii]

Unknown(0)

23503164

To evaluate the prevalence of extended-spectrum cephalosporin (ESC)-resistant Enterobacteriaceae in broiler chickens, 41 rectal samples taken from 4 commercial farms were examined. Desoxycholate hydrogen sulfide lactose agars, supplemented with either 4 mug/ml cefotaxime or 16 mug/ml ceftazidime, were used to screen ESC-resistant bacteria. ESC-resistant bacteria were isolated from all samples. Of the 164 ESC-resistant bacteria (included 4 isolates per a sample), 163 were Escherichia coli, while 1 isolate was identified as Enterobacter cloacae. Extended-spectrum beta-lactamase (ESBL) genes and plasmid-mediated AmpC beta-lactamase genes in the isolates were determined by PCR and sequencing. One AmpC beta-lactamase gene, bla(CMY-2) (66%), and 4 ESBL genes, bla(CTX-M-1) (26%), bla(CTX-M-55) (10%), bla(SHV-5) (4%) and bla(CTX-M-2) (3%), were detected in the E. coli isolates. The epidemiological relationship of the CMY-2 and CTX-M beta-lactamase-producing isolates among the farms was analyzed by pulsed-field gel electrophoresis using the XbaI restriction enzyme. Forty-one (Y1-Y41) and 14 (X1-X14) clusters were found in the CMY-2 and CTX-M-carrying E. coli isolates, respectively. Some clusters included isolates derived from more than 1 farm, indicating some cross-contamination of clonal strains and spread of CMY-2 AmpC beta-lactamase or CTX-M ESBL among the farms.

Kameyama,M., Chuma,T., Yabata,J., Tominaga,K., Iwata,H., Okamoto,K.

Yamaguchi Prefectural Institute of Public Health and Environment, 2-5-67 Aoi, Yamaguchi 753-0821, Japan.

20130315

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=23503164

2013