Journal Article
Print(0)
Journal of virological methods
J.Virol.Methods
15-Sep
222
132
137
CI: Copyright (c) 2015; JID: 8005839; 0 (Drinking Water); OTO: NOTNLM; 2015/02/17 [received]; 2015/05/21 [revised]; 2015/06/14 [accepted]; 2015/06/18 [aheadofprint]; ppublish
Netherlands
1879-0984; 0166-0934
PMID: 26093027
eng
Journal Article; Research Support, Non-U.S. Gov't; IM
10.1016/j.jviromet.2015.06.007 [doi]
Unknown(0)
26093027
According to the risk assessment of the WHO, highly infectious pathogenic viruses like rotaviruses should not be present in large-volume drinking water samples of up to 90 m(3). On the other hand, quantification methods for viruses are only operable in small volumes, and presently no concentration procedure for processing such large volumes has been reported. Therefore, the aim of this study was to demonstrate a procedure for processing viruses in-line of a drinking water pipeline by ultrafiltration (UF) and consecutive further concentration by monolithic filtration (MF) and centrifugal ultrafiltration (CeUF) of viruses to a final 1-mL sample. For testing this concept, the model virus bacteriophage MS2 was spiked continuously in UF instrumentation. Tap water was processed in volumes between 32.4 m(3) (22 h) and 97.7 m(3) (72 h) continuously either in dead-end (DE) or cross-flow (CF) mode. Best results were found by DE-UF over 22 h. The concentration of MS2 was increased from 4.2x10(4) GU/mL (genomic units per milliliter) to 3.2x10(10) GU/mL and from 71 PFU/mL to 2x10(8) PFU/mL as determined by qRT-PCR and plaque assay, respectively.
Elsevier B.V
Kunze,A., Pei,L., Elsasser,D., Niessner,R., Seidel,M.
Institute of Hydrochemistry, Chair of Analytical Chemistry, Technische Universitat Munchen, Marchioninistr. 17, 81377 Munich, Germany.; Institute of Hydrochemistry, Chair of Analytical Chemistry, Technische Universitat Munchen, Marchioninistr. 17, 81377 M
20150618
http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=26093027
2015