Journal Article

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International journal of antimicrobial agents

Int.J.Antimicrob.Agents

Jun

27

6

545

548

JID: 9111860; 0 (Escherichia coli Proteins); EC 3.5.2.- (CTX-M-27, E coli); EC 3.5.2.- (beta-lactamase CTX-M-14); EC 3.5.2.- (beta-lactamase CTX-M-15); EC 3.5.2.6 (beta-Lactamases); 2005/11/02 [received]; 2006/01/08 [accepted]; 2006/05/18 [aheadofprint];

Netherlands

0924-8579; 0924-8579

PMID: 16713187

eng

Journal Article; IM

S0924-8579(06)00092-6 [pii]

Unknown(0)

16713187

We studied the presence of beta-lactamases with an extended spectrum of activity in clinical Escherichia coli isolates from Cairo, Egypt. Forty-six E. coli isolates were collected from patients with urinary tract infections at a university hospital in 2001. Phenotypic characterisation identified a very high extended-spectrum beta-lactamase (ESBL) rate of 60.9%. Pulsed-field gel electrophoresis and plasmid profiles revealed eight different clonal groups. All ESBL producers were polymerase chain reaction-positive for blaTEM and blaCTX-M genes. Within the CTX-M family, three different enzymes, CTX-M-14, CTX-M-15 and CTX-M-27, were found. The ESBL producers carried multiple plasmids and further plasmid-encoded resistances. In several strains, genes for up to six aminoglycoside-modifying enzymes were detected. A linkage to fluoroquinolone resistance was not observed. This study confirms the high rate of ESBLs in Egypt and further demonstrates the worldwide spread of genes coding for CTX-M enzymes in clinical isolates.

Drug Resistance, Bacterial, Escherichia coli/drug effects/enzymology, Escherichia coli Proteins/genetics, Humans, Plasmids, Polymerase Chain Reaction, beta-Lactamases/genetics

Mohamed Al-Agamy,M. H., El-Din Ashour,M. S., Wiegand,I.

Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt.

20060518

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=16713187

2006