Effect of heavy metals and benzene on porphyrin synthesis of human epithelial larynx carcinoma cells (HEp-2)

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Umweltmedizin in Forschung und Praxis

Umweltmed.Forsch.Prax.

2001/

221

230

1430-8681

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Aim of the investigation was to prepare an environmental epidemiological test to detect the effects of pollutants by a pattern of porphyrin excretion in the urine. The task was to test whether lead, mercury, copper, zinc and benzene in environmentally relevant concentrations influence porphyrin synthesis of cells. The model used was the permanent epithelial larynx carcinoma cell line HEp-2, which was stimulated to synthesize porphyrins by adding Î´-amino-levulinic acid (Î´-ALA) in excess. 8- to 4-carboxyporphyrins and protoporphyrin IX were measured both in the cells and in the culture medium by means of high performance liquid cromatography (HPLC). The following pollutant effects were found: lead: extracellular increase of hexacarboxyporphyrin and intracellular decrease of heptacarboxy- and copro-porphyrin; mercury: extracellular decrease of heptacarboxyporphyrin and intracellular increase of coproporphyrin; copper: extracellular decrease of uro-, coproporphyrin and protoporphyrin IX, extracellular increase of heptacarboxyporphyrin and intracellular decrease of uro-, heptacarboxy- and coproporphyrin; zinc: extracellular increase of coproporphyrin; benzene: a tendency to extracellular increase of hexacarboxyporphyrin. The main result is the decrease in the concentrations of porphyrins caused by copper. This result is possibly linked to gastro-intestinal diseases among children due to drinking water from copper water pipes.

aminolevulinic acid, benzene, copper, heavy metal, lead, mercury, porphyrin, protoporphyrin, zinc, article, carcinoma cell, cell culture, culture medium, drinking, gastrointestinal disease, heme synthesis, high performance liquid chromatography, human, larynx carcinoma, pollutant, urinary excretion, water contamination

Wirkung von schwermetallen und benzol auf die porphyrinsynthese von humanen epithelialen larynxkarzinomzellen (HEp-2)

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Tumat,C., Kath,H. -G, Perlewitz,J., Herbarth,O., Manuwald,O.

Herbarth, O., UFZ - Umweltforsch. Ztrm. L.-H. GmbH, Sekt. Expositionsforsch./Epidemiol., Medizinische Fak. der Univ. Leipzig, D-04318 Leipzig, Germany

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2001