Early pulmonary events of nose-only water pipe (shisha) smoking exposure in mice

Journal Article

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Physiological reports

Physiol.Rep.

Mar

10.14814/phy2.12258

United States

2051-817X; 2051-817X

PMID: 25780090

eng

Journal Article

10.14814/phy2.12258 [doi]

Unknown(0)

25780090

Water pipe smoking (WPS) is increasing in popularity and prevalence worldwide. Convincing data suggest that the toxicants in WPS are similar to that of cigarette smoke. However, the underlying pathophysiologic mechanisms related to the early pulmonary events of WPS exposure are not understood. Here, we evaluated the early pulmonary events of nose-only exposure to mainstream WPS generated by commercially available honey flavored "moasel" tobacco. BALB/c mice were exposed to WPS 30 min/day for 5 days. Control mice were exposed using the same protocol to atmospheric air only. We measured airway resistance using forced oscillation technique, and pulmonary inflammation was evaluated histopathologically and by biochemical analysis of bronchoalveolar lavage (BAL) fluid and lung tissue. Lung oxidative stress was evaluated biochemically by measuring the level of reactive oxygen species (ROS), lipid peroxidation (LPO), reduced glutathione (GSH), catalase, and superoxide dismutase (SOD). Mice exposed to WPS showed a significant increase in the number of neutrophils (P

The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society

Nemmar,A., Al Hemeiri,A., Al Hammadi,N., Yuvaraju,P., Beegam,S., Yasin,J., Elwasila,M., Ali,B.H., Adeghate,E.

Department of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates anemmar@uaeu.ac.ae anemmar@hotmail.com.; Department of Physiology, College of Medicine and Health Sciences, United Arab Emirat

PMC4393146

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=25780090

2015