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Environmental microbiology reports

Environ.Microbiol.Rep.

12-Jul

LR: 20160712; CI: (c) 2016; JID: 101499207; OTO: NOTNLM; aheadofprint

1758-2229; 1758-2229

PMID: 27402421

ENG

JOURNAL ARTICLE

10.1111/1758-2229.12440 [doi]

Unknown(0)

27402421

Wastewater treatment plants (WWTP) receiving effluents from food-producing animals and humans may contribute to the spread of ESBL-carrying plasmids. This study was designed to investigate extended-spectrum cephalosporin resistant E. coli strains, CTX-M distributions and the genetic lineage of blaCTX-M -carrying plasmids from urban and slaughterhouse wastewaters. The level of extended-spectrum cephalosporin-resistant E. coli in slaughterhouse wastewater entering the WWTP was negligible compared to that of urban wastewater. The blaCTX-M-1 gene was predominant in slaughterhouse wastewater whereas diverse blaCTX-M genes were encountered in urban wastewater and WWTP outlet. Characterization of the main CTX-M-producing E. coli isolates by antibiotic resistance phenotyping, genotyping and typing of plasmids carrying blaCTX-M genes revealed that blaCTX-M-1 and blaCTX-M-15 genes were harbored by the predominant blaCTX-M-1 IncI1/ST3 and blaCTX-M-15 F31:A4:B1 plasmids, which were recovered from unrelated E. coli genotypes in both slaughterhouse and urban wastewaters. This study highlighted the spread of predominant blaCTX-M-1 and blaCTX-M-15 plasmid lineages in diverse E. coli genotypes from humans and food-producing animals, their mixing in WWTP and final release into the aquatic environment. This could have a serious negative impact on public health and requires further evaluation. This article is protected by copyright. All rights reserved.

Society for Applied Microbiology and John Wiley & Sons Ltd

Dupouy,V., Doublet,B., Arpaillange,N., Praud,K., Bibbal,D., Brugere,H., Oswald,E., Cloeckaert,A., Toutain,P.L., Bousquet-Melou,A.

Toxalim, Universite de Toulouse, INRA, ENVT, INP-Purpan, UPS, Toulouse, France.; UMR1282 Infectiologie et Sante Publique, INRA, F-37380, Nouzilly, France.; UMR1282 Infectiologie et Sante Publique, Universite Francois Rabelais de Tours, F-37000, Tours, Fra

20160712

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=27402421

2016